AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1227240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1227240 MtrunA17_Chr1g0155861 98.261 115 2 0 1 115 1 115 2.46e-82 236
Msa1227240 MtrunA17_Chr8g0392451 97.391 115 3 0 1 115 1 115 7.81e-81 232
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1227240 AT3G01435.1 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa1227240 AT3G01435.4 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa1227240 AT3G01435.2 57.534 146 30 1 1 114 1 146 5.45e-54 166
Msa1227240 AT3G01435.3 70.238 84 25 0 31 114 1 84 6.96e-42 133

Find 55 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTCAAGACAGTGATTT+TGG 0.154549 8_2:-2324744 None:intergenic
GGTTAGGGTTCTTTGAAAAT+TGG 0.180356 8_2:+2322744 Msa1227240:five_prime_UTR
CATATCTCATTTGCTATTCT+TGG 0.183234 8_2:-2326502 None:intergenic
TGAAAATTCAGTCTGTAATT+TGG 0.197049 8_2:+2326644 Msa1227240:three_prime_UTR
TGAATTCATGCAATTAATTA+AGG 0.202211 8_2:+2324759 Msa1227240:CDS
GACTTTCTTGGAAGAATATT+GGG 0.218800 8_2:-2322621 None:intergenic
TGACTTTCTTGGAAGAATAT+TGG 0.221095 8_2:-2322622 None:intergenic
TCAAATGCATATGACTTTCT+TGG 0.235673 8_2:-2322633 None:intergenic
GTTAGGGTTCTTTGAAAATT+GGG 0.254874 8_2:+2322745 Msa1227240:five_prime_UTR
AAAGACAGATTATGAAGTTT+TGG 0.264512 8_2:+2326792 Msa1227240:three_prime_UTR
CCATTTGAAAAGTGTGATTA+TGG 0.272416 8_2:+2326478 Msa1227240:CDS
AAGCTTATTTAATTGTCTAA+TGG 0.277330 8_2:-2322674 None:intergenic
ATAGAGAATTGTGAAGGTTT+TGG 0.292105 8_2:+2324660 Msa1227240:intron
AGCTTATTTAATTGTCTAAT+GGG 0.310583 8_2:-2322673 None:intergenic
GGGAGGAAATAGAGTAAATA+AGG 0.319714 8_2:+2322790 Msa1227240:five_prime_UTR
GTGCAACTTGAATTGCTTGC+AGG 0.330825 8_2:-2326679 None:intergenic
GGAGAAAGACTATTAAGGTT+AGG 0.339063 8_2:+2322728 Msa1227240:five_prime_UTR
TCTTTGAAAATTGGGGAAAA+AGG 0.342786 8_2:+2322753 Msa1227240:five_prime_UTR
ATTTATTTCGAGGGGTCATT+TGG 0.349026 8_2:+2326729 Msa1227240:three_prime_UTR
ACCAAGGAGAAAGACTATTA+AGG 0.372416 8_2:+2322723 Msa1227240:five_prime_UTR
AAAAGGTAGAGCTAGGATTT+GGG 0.386260 8_2:+2322770 Msa1227240:five_prime_UTR
ATAAGCTTAGTGAAAGTGAT+TGG 0.391447 8_2:+2322689 Msa1227240:five_prime_UTR
AAATGAGATATGTTTCAAAA+AGG 0.396887 8_2:+2326513 Msa1227240:CDS
GATGGTGATGTTTGAGTGTT+TGG 0.396997 8_2:-2322587 None:intergenic
ATATCTCATTTGCTATTCTT+GGG 0.398050 8_2:-2326501 None:intergenic
ATTGTTAATTGTTAAGAGAA+AGG 0.407894 8_2:+2322531 None:intergenic
GAGAAAGACTATTAAGGTTA+GGG 0.417995 8_2:+2322729 Msa1227240:five_prime_UTR
ACCTTAATAGTCTTTCTCCT+TGG 0.422251 8_2:-2322724 None:intergenic
GGAATATGTCATGTCACAAT+TGG 0.423342 8_2:+2326537 Msa1227240:CDS
AGCAACATCAATGGATTCAC+AGG 0.432506 8_2:+2324493 Msa1227240:exon
TTTATTTCGAGGGGTCATTT+GGG 0.435975 8_2:+2326730 Msa1227240:three_prime_UTR
TTGTTAATTGTTAAGAGAAA+GGG 0.451821 8_2:+2322532 None:intergenic
GTCTTTGCAATGAAGTTGTC+TGG 0.456591 8_2:-2324517 None:intergenic
AACTTTCATCCTTAGTATCA+AGG 0.457524 8_2:+2322835 Msa1227240:five_prime_UTR
TTTCATCACGCAACGCCACT+TGG 0.479312 8_2:-2326432 None:intergenic
AGGAACCCACCTTGATACTA+AGG 0.497854 8_2:-2322844 None:intergenic
ATAGAGTAAATAAGGAACAT+TGG 0.498437 8_2:+2322798 Msa1227240:five_prime_UTR
TATTTATGTATTTATTTCGA+GGG 0.499343 8_2:+2326720 Msa1227240:three_prime_UTR
TTGATACTAAGGATGAAAGT+TGG 0.502389 8_2:-2322833 None:intergenic
TTATTTATGTATTTATTTCG+AGG 0.502816 8_2:+2326719 Msa1227240:three_prime_UTR
CACAGAGAAAGCAACATCAA+TGG 0.506024 8_2:+2324484 Msa1227240:exon
TTAGGGTTCTTTGAAAATTG+GGG 0.511126 8_2:+2322746 Msa1227240:five_prime_UTR
ATGTCTTTAATCTCAAACAT+AGG 0.521066 8_2:-2326863 None:intergenic
TGCAACTTGAATTGCTTGCA+GGG 0.527566 8_2:-2326678 None:intergenic
TTGTCAATTGTGAACTATCT+CGG 0.532804 8_2:+2322564 Msa1227240:five_prime_UTR
GACGAGCTTGCAAGCCCAGT+TGG 0.535324 8_2:+2324703 Msa1227240:CDS
AAGACTTCAGAATGTCGAAA+AGG 0.556491 8_2:+2324535 Msa1227240:CDS
GGAGCTTGCAGGAGGAGTGA+TGG 0.557814 8_2:+2324681 Msa1227240:CDS
ACTTTCTTGGAAGAATATTG+GGG 0.595084 8_2:-2322620 None:intergenic
AGGTAGAGCTAGGATTTGGG+AGG 0.607159 8_2:+2322773 Msa1227240:five_prime_UTR
AATTCAAGTTGCACTCAACA+AGG 0.612180 8_2:+2326688 Msa1227240:three_prime_UTR
ATTTATGTATTTATTTCGAG+GGG 0.636208 8_2:+2326721 Msa1227240:three_prime_UTR
GTTTGTATAGAGAATTGTGA+AGG 0.675616 8_2:+2324654 Msa1227240:intron
ATTGGAGAGTAAATCAACCA+AGG 0.682126 8_2:+2322707 Msa1227240:five_prime_UTR
GCAACATCAATGGATTCACA+GGG 0.701218 8_2:+2324494 Msa1227240:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATAGAATTATGAAAT+TGG + chr8_2:2322893-2322912 Msa1227240:intron 10.0%
!! ATATTAAAGTATATTAAAGA+AGG + chr8_2:2323766-2323785 Msa1227240:intron 10.0%
!! TATGATATTAATTATTGAAA+TGG - chr8_2:2324863-2324882 None:intergenic 10.0%
!! ATAATTTATCAAATGAATGA+GGG - chr8_2:2322880-2322899 None:intergenic 15.0%
!! TATAATTTATCAAATGAATG+AGG - chr8_2:2322881-2322900 None:intergenic 15.0%
!! TATAGTCTATAATTATTAAG+AGG - chr8_2:2323366-2323385 None:intergenic 15.0%
!! AATAAAGAAATCAACATAAT+GGG - chr8_2:2323931-2323950 None:intergenic 15.0%
!! TAATAAAGAAATCAACATAA+TGG - chr8_2:2323932-2323951 None:intergenic 15.0%
!!! ATTATGTTGATTTCTTTATT+AGG + chr8_2:2323931-2323950 Msa1227240:intron 15.0%
!! TAAAAAAAATCAAGTATGAA+AGG - chr8_2:2324603-2324622 None:intergenic 15.0%
!! ATGAATAAGTGTTTATAAAT+GGG - chr8_2:2325161-2325180 None:intergenic 15.0%
!!! TGATTTTCAATTACAATTAA+CGG + chr8_2:2325568-2325587 Msa1227240:intron 15.0%
!! TTCATTTCATATCTTTAATT+TGG - chr8_2:2326021-2326040 None:intergenic 15.0%
!!! AAACTATTTTTGATAGAAAT+GGG - chr8_2:2326046-2326065 None:intergenic 15.0%
!!! TAAACTATTTTTGATAGAAA+TGG - chr8_2:2326047-2326066 None:intergenic 15.0%
!! AATATTACAACATTATAGTA+GGG - chr8_2:2326356-2326375 None:intergenic 15.0%
!! AAAAATTAGAAAGAAAATGA+AGG - chr8_2:2326403-2326422 None:intergenic 15.0%
!!! ATTTTCTTTCTAATTTTTGT+AGG + chr8_2:2326405-2326424 Msa1227240:intron 15.0%
!! TTATTTATGTATTTATTTCG+AGG + chr8_2:2326719-2326738 Msa1227240:three_prime_UTR 15.0%
!! TATTTATGTATTTATTTCGA+GGG + chr8_2:2326720-2326739 Msa1227240:three_prime_UTR 15.0%
!! AGCTTATTTAATTGTCTAAT+GGG - chr8_2:2322676-2322695 None:intergenic 20.0%
!! AAGCTTATTTAATTGTCTAA+TGG - chr8_2:2322677-2322696 None:intergenic 20.0%
!! AGAATTATGAAATTGGAATT+TGG + chr8_2:2322900-2322919 Msa1227240:intron 20.0%
!! GAATTATGAAATTGGAATTT+GGG + chr8_2:2322901-2322920 Msa1227240:intron 20.0%
!! TTAGTAATATTCATATCTAG+TGG - chr8_2:2323464-2323483 None:intergenic 20.0%
!! TACTTGATAAAAATCTTACA+CGG - chr8_2:2324115-2324134 None:intergenic 20.0%
!! AAAATCTCTAAACAAATTTG+TGG + chr8_2:2324165-2324184 Msa1227240:intron 20.0%
!! AAGATATCAGGAAAAAAATT+AGG + chr8_2:2324351-2324370 Msa1227240:intron 20.0%
!! TGAATTCATGCAATTAATTA+AGG + chr8_2:2324759-2324778 Msa1227240:CDS 20.0%
!!! TAGTTGTTACACAATAATTT+TGG - chr8_2:2324898-2324917 None:intergenic 20.0%
!! AATTATTGTGTAACAACTAT+TGG + chr8_2:2324899-2324918 Msa1227240:intron 20.0%
!! TGAATAAGTGTTTATAAATG+GGG - chr8_2:2325160-2325179 None:intergenic 20.0%
!! CATGAATAAGTGTTTATAAA+TGG - chr8_2:2325162-2325181 None:intergenic 20.0%
!! TTCAATATATAAACTATGCT+TGG + chr8_2:2325210-2325229 Msa1227240:intron 20.0%
!! ACTCAATTACACAAATTTAA+AGG - chr8_2:2325679-2325698 None:intergenic 20.0%
!! GAATATTACAACATTATAGT+AGG - chr8_2:2326357-2326376 None:intergenic 20.0%
!! AAATGAGATATGTTTCAAAA+AGG + chr8_2:2326513-2326532 Msa1227240:CDS 20.0%
!!! ATTTAAGAAGCAAAATTTTC+AGG - chr8_2:2326602-2326621 None:intergenic 20.0%
!! ATTTATGTATTTATTTCGAG+GGG + chr8_2:2326721-2326740 Msa1227240:three_prime_UTR 20.0%
! ATAGAGTAAATAAGGAACAT+TGG + chr8_2:2322798-2322817 Msa1227240:five_prime_UTR 25.0%
!! ATAATATGATGATGTCCAAA+TGG - chr8_2:2323225-2323244 None:intergenic 25.0%
!!! TAGTTGATGTTTTTTATCAG+TGG + chr8_2:2323384-2323403 Msa1227240:intron 25.0%
! TTTATCAGTGGAATGAATAT+TGG + chr8_2:2323396-2323415 Msa1227240:intron 25.0%
!! CTAGTAGAAGATATTGATTA+AGG + chr8_2:2323487-2323506 Msa1227240:intron 25.0%
! ATTGTTATAGCATACTACAT+TGG + chr8_2:2323664-2323683 Msa1227240:intron 25.0%
!! ATTGGCAGTACAAATTATTT+TGG + chr8_2:2323682-2323701 Msa1227240:intron 25.0%
!! ATTTCCCTTTTTGTAAAATG+TGG + chr8_2:2324030-2324049 Msa1227240:intron 25.0%
!! TTTTCTAAGCAGTAAACTAA+TGG - chr8_2:2324237-2324256 None:intergenic 25.0%
! AATCTTGCCAAAATATTGTA+GGG - chr8_2:2324399-2324418 None:intergenic 25.0%
! TAATCTTGCCAAAATATTGT+AGG - chr8_2:2324400-2324419 None:intergenic 25.0%
!! TTTTTATTACAAGATTGCAC+TGG + chr8_2:2324812-2324831 Msa1227240:intron 25.0%
! TATATAACTATAGATGCAAG+TGG + chr8_2:2324835-2324854 Msa1227240:intron 25.0%
! GAATAAGTGTTTATAAATGG+GGG - chr8_2:2325159-2325178 None:intergenic 25.0%
! TATGTTGCATACAAATGAAA+GGG - chr8_2:2325270-2325289 None:intergenic 25.0%
!!! CTATTTCACATAACTGATTT+TGG + chr8_2:2325363-2325382 Msa1227240:intron 25.0%
! GACTATTAGCACTTAAAAAA+TGG + chr8_2:2325708-2325727 Msa1227240:intron 25.0%
! TTCAAATACCAAACTTACAA+AGG - chr8_2:2325842-2325861 None:intergenic 25.0%
! TAATGAACATTAGATTTCTC+AGG - chr8_2:2325866-2325885 None:intergenic 25.0%
! CTATCAAAAATAGTTTACCT+TGG + chr8_2:2326050-2326069 Msa1227240:intron 25.0%
! AAACAACTAAGTACATGTTT+GGG + chr8_2:2326077-2326096 Msa1227240:intron 25.0%
! AACACAAATAATTCTTCTAG+AGG - chr8_2:2326157-2326176 None:intergenic 25.0%
! TATATACTCCAATAACTAAC+AGG - chr8_2:2326218-2326237 None:intergenic 25.0%
!!! ATATCTCATTTGCTATTCTT+GGG - chr8_2:2326504-2326523 None:intergenic 25.0%
! TGAAAATTCAGTCTGTAATT+TGG + chr8_2:2326644-2326663 Msa1227240:three_prime_UTR 25.0%
!! TAATCTGTCTTTTACATGTT+TGG - chr8_2:2326784-2326803 None:intergenic 25.0%
!! AAAGACAGATTATGAAGTTT+TGG + chr8_2:2326792-2326811 Msa1227240:three_prime_UTR 25.0%
TTGTCAATTGTGAACTATCT+CGG + chr8_2:2322564-2322583 Msa1227240:five_prime_UTR 30.0%
ACTTTCTTGGAAGAATATTG+GGG - chr8_2:2322623-2322642 None:intergenic 30.0%
GACTTTCTTGGAAGAATATT+GGG - chr8_2:2322624-2322643 None:intergenic 30.0%
TGACTTTCTTGGAAGAATAT+TGG - chr8_2:2322625-2322644 None:intergenic 30.0%
TCAAATGCATATGACTTTCT+TGG - chr8_2:2322636-2322655 None:intergenic 30.0%
ATAAGCTTAGTGAAAGTGAT+TGG + chr8_2:2322689-2322708 Msa1227240:five_prime_UTR 30.0%
GAGAAAGACTATTAAGGTTA+GGG + chr8_2:2322729-2322748 Msa1227240:five_prime_UTR 30.0%
! GTTAGGGTTCTTTGAAAATT+GGG + chr8_2:2322745-2322764 Msa1227240:five_prime_UTR 30.0%
! TTAGGGTTCTTTGAAAATTG+GGG + chr8_2:2322746-2322765 Msa1227240:five_prime_UTR 30.0%
! TCTTTGAAAATTGGGGAAAA+AGG + chr8_2:2322753-2322772 Msa1227240:five_prime_UTR 30.0%
TTGATACTAAGGATGAAAGT+TGG - chr8_2:2322836-2322855 None:intergenic 30.0%
AACTTTCATCCTTAGTATCA+AGG + chr8_2:2322835-2322854 Msa1227240:five_prime_UTR 30.0%
! AAAGCATTCAAATACATCGA+AGG - chr8_2:2323100-2323119 None:intergenic 30.0%
CAGATTAAGATTGCACATTA+AGG + chr8_2:2323191-2323210 Msa1227240:intron 30.0%
ATTAAGGTCATAACTCCATT+TGG + chr8_2:2323207-2323226 Msa1227240:intron 30.0%
AAGTGAATAAGAATGAAGTC+TGG + chr8_2:2323260-2323279 Msa1227240:intron 30.0%
CAACAGATTGTATCGAAAAT+AGG + chr8_2:2323317-2323336 Msa1227240:intron 30.0%
AAATAGGATAGAACATGCAA+TGG + chr8_2:2323333-2323352 Msa1227240:intron 30.0%
TAGGTAGTGTTTAGATAATG+TGG + chr8_2:2323533-2323552 Msa1227240:intron 30.0%
AGGTAGTGTTTAGATAATGT+GGG + chr8_2:2323534-2323553 Msa1227240:intron 30.0%
CAATAATAAAGCTTTGCAAG+TGG - chr8_2:2323648-2323667 None:intergenic 30.0%
! ACTAGAAATTGATTCCAACT+TGG + chr8_2:2323852-2323871 Msa1227240:intron 30.0%
ATAAACATAGAATGCCAAGT+TGG - chr8_2:2323869-2323888 None:intergenic 30.0%
ATTCTATGTTTATCCCGATT+TGG + chr8_2:2323876-2323895 Msa1227240:intron 30.0%
TTCTATGTTTATCCCGATTT+GGG + chr8_2:2323877-2323896 Msa1227240:intron 30.0%
!! TAAGCTATTTTGCTGTCAAT+CGG + chr8_2:2323956-2323975 Msa1227240:intron 30.0%
! TCAGCCACATTTTACAAAAA+GGG - chr8_2:2324037-2324056 None:intergenic 30.0%
! ATCAGCCACATTTTACAAAA+AGG - chr8_2:2324038-2324057 None:intergenic 30.0%
TTGATAAAAATCTTACACGG+CGG - chr8_2:2324112-2324131 None:intergenic 30.0%
! AACTAATGGTGTGTTTGTTT+TGG - chr8_2:2324223-2324242 None:intergenic 30.0%
GTTTGTATAGAGAATTGTGA+AGG + chr8_2:2324654-2324673 Msa1227240:intron 30.0%
! ATAGAGAATTGTGAAGGTTT+TGG + chr8_2:2324660-2324679 Msa1227240:intron 30.0%
! TTTTGGACAAGATCTTTTCT+AGG - chr8_2:2324730-2324749 None:intergenic 30.0%
! TGAATTCAAGACAGTGATTT+TGG - chr8_2:2324747-2324766 None:intergenic 30.0%
AATTATTGAGAGACAAAGCA+AGG - chr8_2:2324789-2324808 None:intergenic 30.0%
CTAACTCATCCTTACAAAAT+CGG - chr8_2:2325101-2325120 None:intergenic 30.0%
ATTGAAATGTCTACGTACAT+CGG - chr8_2:2325196-2325215 None:intergenic 30.0%
ATGGGAATCACCATAAAAAA+GGG - chr8_2:2325245-2325264 None:intergenic 30.0%
TATGGGAATCACCATAAAAA+AGG - chr8_2:2325246-2325265 None:intergenic 30.0%
CATACAAATGAAAGGGTTAT+GGG - chr8_2:2325263-2325282 None:intergenic 30.0%
GTATGTTGCATACAAATGAA+AGG - chr8_2:2325271-2325290 None:intergenic 30.0%
TGAAATAGCAAATACTGTCA+CGG - chr8_2:2325351-2325370 None:intergenic 30.0%
ATGTAATCAATAGCAGATTG+CGG - chr8_2:2325400-2325419 None:intergenic 30.0%
AACAGATAGAATTGTGATCA+AGG - chr8_2:2325549-2325568 None:intergenic 30.0%
TACCTAAAAAGAGGATAAAG+CGG - chr8_2:2325602-2325621 None:intergenic 30.0%
CAGATTTGATACCTAAAAAG+AGG - chr8_2:2325611-2325630 None:intergenic 30.0%
TATCACAACCAAACTTACAA+AGG - chr8_2:2325811-2325830 None:intergenic 30.0%
AAACAACACTAGTTATGTGA+TGG + chr8_2:2325965-2325984 Msa1227240:intron 30.0%
CAAACATGTACTTAGTTGTT+TGG - chr8_2:2326078-2326097 None:intergenic 30.0%
CAAACAACTAAGTACATGTT+TGG + chr8_2:2326076-2326095 Msa1227240:intron 30.0%
TAAGTACATGTTTGGGTAAT+TGG + chr8_2:2326084-2326103 Msa1227240:intron 30.0%
ACTAAACCTGTTAGCAATTA+GGG - chr8_2:2326286-2326305 None:intergenic 30.0%
AACTAAACCTGTTAGCAATT+AGG - chr8_2:2326287-2326306 None:intergenic 30.0%
CTAACAGGTTTAGTTATTCA+GGG + chr8_2:2326292-2326311 Msa1227240:intron 30.0%
! CCATAATCACACTTTTCAAA+TGG - chr8_2:2326481-2326500 None:intergenic 30.0%
! CCATTTGAAAAGTGTGATTA+TGG + chr8_2:2326478-2326497 Msa1227240:CDS 30.0%
!! CATATCTCATTTGCTATTCT+TGG - chr8_2:2326505-2326524 None:intergenic 30.0%
TGAGATATGTTTCAAAAAGG+TGG + chr8_2:2326516-2326535 Msa1227240:CDS 30.0%
TATTGGGGAAAAAGCTATGA+TGG - chr8_2:2322608-2322627 None:intergenic 35.0%
ATTGGAGAGTAAATCAACCA+AGG + chr8_2:2322707-2322726 Msa1227240:five_prime_UTR 35.0%
ACCAAGGAGAAAGACTATTA+AGG + chr8_2:2322723-2322742 Msa1227240:five_prime_UTR 35.0%
ACCTTAATAGTCTTTCTCCT+TGG - chr8_2:2322727-2322746 None:intergenic 35.0%
GGAGAAAGACTATTAAGGTT+AGG + chr8_2:2322728-2322747 Msa1227240:five_prime_UTR 35.0%
! GGTTAGGGTTCTTTGAAAAT+TGG + chr8_2:2322744-2322763 Msa1227240:five_prime_UTR 35.0%
AAAAAGGTAGAGCTAGGATT+TGG + chr8_2:2322769-2322788 Msa1227240:five_prime_UTR 35.0%
AAAAGGTAGAGCTAGGATTT+GGG + chr8_2:2322770-2322789 Msa1227240:five_prime_UTR 35.0%
GGGAGGAAATAGAGTAAATA+AGG + chr8_2:2322790-2322809 Msa1227240:five_prime_UTR 35.0%
TTTCATCCTTAGTATCAAGG+TGG + chr8_2:2322838-2322857 Msa1227240:intron 35.0%
TTCATCCTTAGTATCAAGGT+GGG + chr8_2:2322839-2322858 Msa1227240:intron 35.0%
ATCAAATGAATGAGGGATGA+AGG - chr8_2:2322873-2322892 None:intergenic 35.0%
ATGTGCAATCTTAATCTGCA+AGG - chr8_2:2323189-2323208 None:intergenic 35.0%
ATACAGTGTGAACCTGATAT+AGG + chr8_2:2323432-2323451 Msa1227240:intron 35.0%
TACAGTGTGAACCTGATATA+GGG + chr8_2:2323433-2323452 Msa1227240:intron 35.0%
TAGTTGCAATTTGTGCAACT+AGG + chr8_2:2323514-2323533 Msa1227240:intron 35.0%
GGTAGTGTTTAGATAATGTG+GGG + chr8_2:2323535-2323554 Msa1227240:intron 35.0%
!! TTTTGCTGTCAATCGGATAT+CGG + chr8_2:2323963-2323982 Msa1227240:intron 35.0%
! TGTCTAGAGTCTAGAATTCT+CGG + chr8_2:2323994-2324013 Msa1227240:intron 35.0%
AGTGTTGCGTAAAGATTGAT+TGG + chr8_2:2324264-2324283 Msa1227240:intron 35.0%
AAGACTTCAGAATGTCGAAA+AGG + chr8_2:2324535-2324554 Msa1227240:CDS 35.0%
ATTGGAAATTCTGCCTTCAT+TGG + chr8_2:2324917-2324936 Msa1227240:intron 35.0%
TTCATTGTGTTGGGTTTGAA+GGG + chr8_2:2325001-2325020 Msa1227240:intron 35.0%
GTCATATCTCTAAGTGATGT+GGG - chr8_2:2325038-2325057 None:intergenic 35.0%
CCAATGCTTTATAAACACAC+AGG - chr8_2:2325060-2325079 None:intergenic 35.0%
CCTGTGTGTTTATAAAGCAT+TGG + chr8_2:2325057-2325076 Msa1227240:intron 35.0%
! CGATTTTGTAAGGATGAGTT+AGG + chr8_2:2325099-2325118 Msa1227240:intron 35.0%
ATCACCATAAAAAAGGGGAA+TGG - chr8_2:2325239-2325258 None:intergenic 35.0%
TGGGAATCACCATAAAAAAG+GGG - chr8_2:2325244-2325263 None:intergenic 35.0%
GCATACAAATGAAAGGGTTA+TGG - chr8_2:2325264-2325283 None:intergenic 35.0%
GAAATAGCAAATACTGTCAC+GGG - chr8_2:2325350-2325369 None:intergenic 35.0%
! ATCCGCTTTATCCTCTTTTT+AGG + chr8_2:2325597-2325616 Msa1227240:intron 35.0%
! AAAATTGTCCTGTTTCTCCA+TGG + chr8_2:2325740-2325759 Msa1227240:intron 35.0%
AAGCATGAAAATACACACCA+TGG - chr8_2:2325760-2325779 None:intergenic 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr8_2:2325800-2325819 Msa1227240:intron 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr8_2:2325800-2325819 Msa1227240:intron 35.0%
TACTTAGTTGTTTGGATCCA+AGG - chr8_2:2326070-2326089 None:intergenic 35.0%
TAATTGGTGCAAACTACAGA+GGG + chr8_2:2326100-2326119 Msa1227240:intron 35.0%
TCTTCTGAGTGCATTGTATT+TGG + chr8_2:2326123-2326142 Msa1227240:intron 35.0%
AGTTCTGTCCTGTTAGTTAT+TGG + chr8_2:2326207-2326226 Msa1227240:intron 35.0%
GCTAACAGGTTTAGTTATTC+AGG + chr8_2:2326291-2326310 Msa1227240:intron 35.0%
! ATTTTTGTAGGACATCCAAG+TGG + chr8_2:2326417-2326436 Msa1227240:intron 35.0%
GGAATATGTCATGTCACAAT+TGG + chr8_2:2326537-2326556 Msa1227240:CDS 35.0%
!! AATTCAAGTTGCACTCAACA+AGG + chr8_2:2326688-2326707 Msa1227240:three_prime_UTR 35.0%
ATTTATTTCGAGGGGTCATT+TGG + chr8_2:2326729-2326748 Msa1227240:three_prime_UTR 35.0%
TTTATTTCGAGGGGTCATTT+GGG + chr8_2:2326730-2326749 Msa1227240:three_prime_UTR 35.0%
! GATGGTGATGTTTGAGTGTT+TGG - chr8_2:2322590-2322609 None:intergenic 40.0%
! CTGAGTTGCTTTCATGTGTA+TGG + chr8_2:2323044-2323063 Msa1227240:intron 40.0%
! AGTTGCTTTCATGTGTATGG+AGG + chr8_2:2323047-2323066 Msa1227240:intron 40.0%
CGATACAATCTGTTGCATGA+AGG - chr8_2:2323312-2323331 None:intergenic 40.0%
TAGTGGCATTACCCTATATC+AGG - chr8_2:2323447-2323466 None:intergenic 40.0%
TGGGGAAGATTAGTAAGCTA+AGG + chr8_2:2323553-2323572 Msa1227240:intron 40.0%
! CCTAAGGTTTTTCACTGTGT+CGG - chr8_2:2323586-2323605 None:intergenic 40.0%
CAGTGAAAAACCTTAGGTGT+CGG + chr8_2:2323589-2323608 Msa1227240:intron 40.0%
TACATTGTAACCGACACCTA+AGG - chr8_2:2323602-2323621 None:intergenic 40.0%
!! TTAGGTGTCGGTTACAATGT+AGG + chr8_2:2323601-2323620 Msa1227240:intron 40.0%
! AAGAAGGCATGTGATGCTAA+CGG + chr8_2:2323782-2323801 Msa1227240:intron 40.0%
TCTGCATAATCCAACTTGTG+CGG + chr8_2:2324322-2324341 Msa1227240:intron 40.0%
! TACCGCTCCCTACAATATTT+TGG + chr8_2:2324389-2324408 Msa1227240:intron 40.0%
TGCCAAAATATTGTAGGGAG+CGG - chr8_2:2324394-2324413 None:intergenic 40.0%
CACAGAGAAAGCAACATCAA+TGG + chr8_2:2324484-2324503 Msa1227240:exon 40.0%
! AGCAACATCAATGGATTCAC+AGG + chr8_2:2324493-2324512 Msa1227240:exon 40.0%
! GCAACATCAATGGATTCACA+GGG + chr8_2:2324494-2324513 Msa1227240:exon 40.0%
GTCTTTGCAATGAAGTTGTC+TGG - chr8_2:2324520-2324539 None:intergenic 40.0%
! GGAAAGTTGTGTCACTCATA+TGG - chr8_2:2324582-2324601 None:intergenic 40.0%
!!! GATCTTTTCTAGGACCAACT+GGG - chr8_2:2324720-2324739 None:intergenic 40.0%
!!! AGATCTTTTCTAGGACCAAC+TGG - chr8_2:2324721-2324740 None:intergenic 40.0%
GAAACCCAATGGACACAATT+AGG - chr8_2:2324959-2324978 None:intergenic 40.0%
!! AACACAATGAAGGTGTTGGA+GGG - chr8_2:2324992-2325011 None:intergenic 40.0%
TCCAACACCTTCATTGTGTT+GGG + chr8_2:2324992-2325011 Msa1227240:intron 40.0%
CTTCAAACCCAACACAATGA+AGG - chr8_2:2325002-2325021 None:intergenic 40.0%
CTTCATTGTGTTGGGTTTGA+AGG + chr8_2:2325000-2325019 Msa1227240:intron 40.0%
TCATTGTGTTGGGTTTGAAG+GGG + chr8_2:2325002-2325021 Msa1227240:intron 40.0%
GGTCATATCTCTAAGTGATG+TGG - chr8_2:2325039-2325058 None:intergenic 40.0%
!! AAAATCGGCTTGTAAGGTGA+GGG - chr8_2:2325086-2325105 None:intergenic 40.0%
!! CCTTACAAAATCGGCTTGTA+AGG - chr8_2:2325092-2325111 None:intergenic 40.0%
!! CCTTACAAGCCGATTTTGTA+AGG + chr8_2:2325089-2325108 Msa1227240:intron 40.0%
ACCATAAAAAAGGGGAATGG+AGG - chr8_2:2325236-2325255 None:intergenic 40.0%
! AAATGGTGGAAACCACATTG+CGG - chr8_2:2325326-2325345 None:intergenic 40.0%
CAAATACTGTCACGGGAAAA+TGG - chr8_2:2325343-2325362 None:intergenic 40.0%
AACACGATCTTGATCAGAGT+AGG + chr8_2:2325469-2325488 Msa1227240:intron 40.0%
AATACACACCATGGAGAAAC+AGG - chr8_2:2325751-2325770 None:intergenic 40.0%
TGGCACATTCAATCTGTCTT+GGG - chr8_2:2325934-2325953 None:intergenic 40.0%
GTAATTGGTGCAAACTACAG+AGG + chr8_2:2326099-2326118 Msa1227240:intron 40.0%
GTTTCTCCCTAATTGCTAAC+AGG + chr8_2:2326277-2326296 Msa1227240:intron 40.0%
TGCAACTTGAATTGCTTGCA+GGG - chr8_2:2326681-2326700 None:intergenic 40.0%
TTGGGGAAAAAGGTAGAGCT+AGG + chr8_2:2322763-2322782 Msa1227240:five_prime_UTR 45.0%
AGGAACCCACCTTGATACTA+AGG - chr8_2:2322847-2322866 None:intergenic 45.0%
TGAATGAGGGATGAAGGAAG+AGG - chr8_2:2322867-2322886 None:intergenic 45.0%
CCGACACAGTGAAAAACCTT+AGG + chr8_2:2323583-2323602 Msa1227240:intron 45.0%
TTACAATGTAGGCAGGCAAG+TGG + chr8_2:2323612-2323631 Msa1227240:intron 45.0%
TACAATGTAGGCAGGCAAGT+GGG + chr8_2:2323613-2323632 Msa1227240:intron 45.0%
TTACCGACACCCGAGTAATT+TGG + chr8_2:2323706-2323725 Msa1227240:intron 45.0%
ACTGCGTCTGCCAAATTACT+CGG - chr8_2:2323719-2323738 None:intergenic 45.0%
TCACAGTGAGTTCCCAAATC+GGG - chr8_2:2323892-2323911 None:intergenic 45.0%
CTCACAGTGAGTTCCCAAAT+CGG - chr8_2:2323893-2323912 None:intergenic 45.0%
! TCTTTAAGCACCGCACAAGT+TGG - chr8_2:2324335-2324354 None:intergenic 45.0%
GTGCGGTGCTTAAAGATATC+AGG + chr8_2:2324339-2324358 Msa1227240:intron 45.0%
AGTCGCCTAATTGTGTCCAT+TGG + chr8_2:2324951-2324970 Msa1227240:intron 45.0%
GTCGCCTAATTGTGTCCATT+GGG + chr8_2:2324952-2324971 Msa1227240:intron 45.0%
GTGCAATGAAGGAAACCCAA+TGG - chr8_2:2324970-2324989 None:intergenic 45.0%
!! CAACACAATGAAGGTGTTGG+AGG - chr8_2:2324993-2325012 None:intergenic 45.0%
CTCCAACACCTTCATTGTGT+TGG + chr8_2:2324991-2325010 Msa1227240:intron 45.0%
! ACCCAACACAATGAAGGTGT+TGG - chr8_2:2324996-2325015 None:intergenic 45.0%
!! CAAAATCGGCTTGTAAGGTG+AGG - chr8_2:2325087-2325106 None:intergenic 45.0%
AGTTAGGCCCCGATATAAAC+CGG + chr8_2:2325115-2325134 Msa1227240:intron 45.0%
! TCACAAGCCGGTTTATATCG+GGG - chr8_2:2325125-2325144 None:intergenic 45.0%
! CTCACAAGCCGGTTTATATC+GGG - chr8_2:2325126-2325145 None:intergenic 45.0%
! CCTCACAAGCCGGTTTATAT+CGG - chr8_2:2325127-2325146 None:intergenic 45.0%
! TACGTACATCGGTTGAGAGA+TGG - chr8_2:2325185-2325204 None:intergenic 45.0%
!!! GCCTCCATTCCCCTTTTTTA+TGG + chr8_2:2325232-2325251 Msa1227240:intron 45.0%
GACTTCTTTCAACCGCAATG+TGG + chr8_2:2325311-2325330 Msa1227240:intron 45.0%
ATACTGTCACGGGAAAATGG+TGG - chr8_2:2325340-2325359 None:intergenic 45.0%
CTTGATCAGAGTAGGTGAGT+AGG + chr8_2:2325477-2325496 Msa1227240:intron 45.0%
GTGGCACATTCAATCTGTCT+TGG - chr8_2:2325935-2325954 None:intergenic 45.0%
!! GTGTTGTTTGAGCAGTACTG+TGG - chr8_2:2325954-2325973 None:intergenic 45.0%
TATACGTGTGTGTGTGTGTG+TGG + chr8_2:2326234-2326253 Msa1227240:intron 45.0%
! GTGTGTGGTTTTCGATGACA+TGG + chr8_2:2326249-2326268 Msa1227240:intron 45.0%
GTGCAACTTGAATTGCTTGC+AGG - chr8_2:2326682-2326701 None:intergenic 45.0%
AGGTAGAGCTAGGATTTGGG+AGG + chr8_2:2322773-2322792 Msa1227240:five_prime_UTR 50.0%
!! GTGTCGGTTACAATGTAGGC+AGG + chr8_2:2323605-2323624 Msa1227240:intron 50.0%
CTGCCAAATTACTCGGGTGT+CGG - chr8_2:2323712-2323731 None:intergenic 50.0%
CTGCGTCTGCCAAATTACTC+GGG - chr8_2:2323718-2323737 None:intergenic 50.0%
!!! GTGAAGGTTTTGGAGCTTGC+AGG + chr8_2:2324670-2324689 Msa1227240:CDS 50.0%
!!! AAGGTTTTGGAGCTTGCAGG+AGG + chr8_2:2324673-2324692 Msa1227240:CDS 50.0%
CACAATTAGGCGACTAGAGG+CGG - chr8_2:2324946-2324965 None:intergenic 50.0%
GGACACAATTAGGCGACTAG+AGG - chr8_2:2324949-2324968 None:intergenic 50.0%
TTGTGTTGGGTTTGAAGGGG+TGG + chr8_2:2325005-2325024 Msa1227240:intron 50.0%
!! CCGATATAAACCGGCTTGTG+AGG + chr8_2:2325124-2325143 Msa1227240:intron 50.0%
!! ATAAACCGGCTTGTGAGGTG+AGG + chr8_2:2325129-2325148 Msa1227240:intron 50.0%
TTTCATCACGCAACGCCACT+TGG - chr8_2:2326435-2326454 None:intergenic 50.0%
CTAGAGGCGGAGTCCAATGA+AGG - chr8_2:2324933-2324952 None:intergenic 55.0%
! GGTGTTGGAGGGTGCAATGA+AGG - chr8_2:2324981-2325000 None:intergenic 55.0%
GCAATCCTCACCTCACAAGC+CGG - chr8_2:2325137-2325156 None:intergenic 55.0%
GGAGCTTGCAGGAGGAGTGA+TGG + chr8_2:2324681-2324700 Msa1227240:CDS 60.0%
GACGAGCTTGCAAGCCCAGT+TGG + chr8_2:2324703-2324722 Msa1227240:CDS 60.0%
GTAGGCAGGCAAGTGGGACG+CGG + chr8_2:2323619-2323638 Msa1227240:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 2322544 2326881 2322544 ID=Msa1227240;Name=Msa1227240
chr8_2 mRNA 2322544 2326881 2322544 ID=Msa1227240-mRNA-1;Parent=Msa1227240;Name=Msa1227240-mRNA-1;_AED=0.15;_eAED=0.15;_QI=346|1|1|1|0.66|0.5|4|278|115
chr8_2 exon 2322544 2322856 2322544 ID=Msa1227240-mRNA-1:exon:603;Parent=Msa1227240-mRNA-1
chr8_2 exon 2324470 2324556 2324470 ID=Msa1227240-mRNA-1:exon:604;Parent=Msa1227240-mRNA-1
chr8_2 exon 2324664 2324780 2324664 ID=Msa1227240-mRNA-1:exon:605;Parent=Msa1227240-mRNA-1
chr8_2 exon 2326427 2326881 2326427 ID=Msa1227240-mRNA-1:exon:606;Parent=Msa1227240-mRNA-1
chr8_2 five_prime_UTR 2322544 2322856 2322544 ID=Msa1227240-mRNA-1:five_prime_utr;Parent=Msa1227240-mRNA-1
chr8_2 five_prime_UTR 2324470 2324502 2324470 ID=Msa1227240-mRNA-1:five_prime_utr;Parent=Msa1227240-mRNA-1
chr8_2 CDS 2324503 2324556 2324503 ID=Msa1227240-mRNA-1:cds;Parent=Msa1227240-mRNA-1
chr8_2 CDS 2324664 2324780 2324664 ID=Msa1227240-mRNA-1:cds;Parent=Msa1227240-mRNA-1
chr8_2 CDS 2326427 2326603 2326427 ID=Msa1227240-mRNA-1:cds;Parent=Msa1227240-mRNA-1
chr8_2 three_prime_UTR 2326604 2326881 2326604 ID=Msa1227240-mRNA-1:three_prime_utr;Parent=Msa1227240-mRNA-1
Gene Sequence

>Msa1227240

ATGGATTCACAGGGCCAGACAACTTCATTGCAAAGACTTCAGAATGTCGAAAAGAGAATTGTGAAGGTTTTGGAGCTTGCAGGAGGAGTGATGGACGAGCTTGCAAGCCCAGTTGGTCCTAGAAAAGATCTTGTCCAAAATCACTGTCTTGAATTCATGCAATTAATTAAGGACATCCAAGTGGCGTTGCGTGATGAAATCAAAAGTGCTTGTGAATATCGTCCATTTGAAAAGTGTGATTATGGCCCAAGAATAGCAAATGAGATATGTTTCAAAAAGGTGGAATATGTCATGTCACAATTGGAAGCAATGAAACAAACTATAGATGAGTATAATGCAGCAGCCTGA
Protein sequence

>Msa1227240

MDSQGQTTSLQRLQNVEKRIVKVLELAGGVMDELASPVGPRKDLVQNHCLEFMQLIKDIQVALRDEIKSACEYRPFEKCDYGPRIANEICFKKVEYVMSQLEAMKQTIDEYNAAA*