AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0003230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0003230 MtrunA17_Chr1g0155861 98.261 115 2 0 1 115 1 115 2.46e-82 236
Msa0003230 MtrunA17_Chr8g0392451 97.391 115 3 0 1 115 1 115 7.81e-81 232
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0003230 AT3G01435.1 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa0003230 AT3G01435.4 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa0003230 AT3G01435.2 57.534 146 30 1 1 114 1 146 5.45e-54 166
Msa0003230 AT3G01435.3 70.238 84 25 0 31 114 1 84 6.96e-42 133

Find 55 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTCAAGACAGTGATTT+TGG 0.154549 1_1:-5754655 None:intergenic
GGTTAGGGTTCTTTGAAAAT+TGG 0.180356 1_1:+5752655 Msa0003230:five_prime_UTR
CATATCTCATTTGCTATTCT+TGG 0.183234 1_1:-5756413 None:intergenic
TGAAAATTCAGTCTGTAATT+TGG 0.197049 1_1:+5756555 Msa0003230:three_prime_UTR
TGAATTCATGCAATTAATTA+AGG 0.202211 1_1:+5754670 Msa0003230:CDS
GACTTTCTTGGAAGAATATT+GGG 0.218800 1_1:-5752532 None:intergenic
TGACTTTCTTGGAAGAATAT+TGG 0.221095 1_1:-5752533 None:intergenic
TCAAATGCATATGACTTTCT+TGG 0.235673 1_1:-5752544 None:intergenic
GTTAGGGTTCTTTGAAAATT+GGG 0.254874 1_1:+5752656 Msa0003230:five_prime_UTR
AAAGACAGATTATGAAGTTT+TGG 0.264512 1_1:+5756703 Msa0003230:three_prime_UTR
CCATTTGAAAAGTGTGATTA+TGG 0.272416 1_1:+5756389 Msa0003230:CDS
AAGCTTATTTAATTGTCTAA+TGG 0.277330 1_1:-5752585 None:intergenic
ATAGAGAATTGTGAAGGTTT+TGG 0.292105 1_1:+5754571 Msa0003230:intron
AGCTTATTTAATTGTCTAAT+GGG 0.310583 1_1:-5752584 None:intergenic
GGGAGGAAATAGAGTAAATA+AGG 0.319714 1_1:+5752701 Msa0003230:five_prime_UTR
GTGCAACTTGAATTGCTTGC+AGG 0.330825 1_1:-5756590 None:intergenic
GGAGAAAGACTATTAAGGTT+AGG 0.339063 1_1:+5752639 Msa0003230:five_prime_UTR
TCTTTGAAAATTGGGGAAAA+AGG 0.342786 1_1:+5752664 Msa0003230:five_prime_UTR
ATTTATTTCGAGGGGTCATT+TGG 0.349026 1_1:+5756640 Msa0003230:three_prime_UTR
ACCAAGGAGAAAGACTATTA+AGG 0.372416 1_1:+5752634 Msa0003230:five_prime_UTR
AAAAGGTAGAGCTAGGATTT+GGG 0.386260 1_1:+5752681 Msa0003230:five_prime_UTR
ATAAGCTTAGTGAAAGTGAT+TGG 0.391447 1_1:+5752600 Msa0003230:five_prime_UTR
AAATGAGATATGTTTCAAAA+AGG 0.396887 1_1:+5756424 Msa0003230:CDS
GATGGTGATGTTTGAGTGTT+TGG 0.396997 1_1:-5752498 None:intergenic
ATATCTCATTTGCTATTCTT+GGG 0.398050 1_1:-5756412 None:intergenic
ATTGTTAATTGTTAAGAGAA+AGG 0.407894 1_1:+5752442 None:intergenic
GAGAAAGACTATTAAGGTTA+GGG 0.417995 1_1:+5752640 Msa0003230:five_prime_UTR
ACCTTAATAGTCTTTCTCCT+TGG 0.422251 1_1:-5752635 None:intergenic
GGAATATGTCATGTCACAAT+TGG 0.423342 1_1:+5756448 Msa0003230:CDS
AGCAACATCAATGGATTCAC+AGG 0.432506 1_1:+5754404 Msa0003230:exon
TTTATTTCGAGGGGTCATTT+GGG 0.435975 1_1:+5756641 Msa0003230:three_prime_UTR
TTGTTAATTGTTAAGAGAAA+GGG 0.451821 1_1:+5752443 None:intergenic
GTCTTTGCAATGAAGTTGTC+TGG 0.456591 1_1:-5754428 None:intergenic
AACTTTCATCCTTAGTATCA+AGG 0.457524 1_1:+5752746 Msa0003230:five_prime_UTR
TTTCATCACGCAACGCCACT+TGG 0.479312 1_1:-5756343 None:intergenic
AGGAACCCACCTTGATACTA+AGG 0.497854 1_1:-5752755 None:intergenic
ATAGAGTAAATAAGGAACAT+TGG 0.498437 1_1:+5752709 Msa0003230:five_prime_UTR
TATTTATGTATTTATTTCGA+GGG 0.499343 1_1:+5756631 Msa0003230:three_prime_UTR
TTGATACTAAGGATGAAAGT+TGG 0.502389 1_1:-5752744 None:intergenic
TTATTTATGTATTTATTTCG+AGG 0.502816 1_1:+5756630 Msa0003230:three_prime_UTR
CACAGAGAAAGCAACATCAA+TGG 0.506024 1_1:+5754395 Msa0003230:exon
TTAGGGTTCTTTGAAAATTG+GGG 0.511126 1_1:+5752657 Msa0003230:five_prime_UTR
ATGTCTTTAATCTCAAACAT+AGG 0.521066 1_1:-5756774 None:intergenic
TGCAACTTGAATTGCTTGCA+GGG 0.527566 1_1:-5756589 None:intergenic
TTGTCAATTGTGAACTATCT+CGG 0.532804 1_1:+5752475 Msa0003230:five_prime_UTR
GACGAGCTTGCAAGCCCAGT+TGG 0.535324 1_1:+5754614 Msa0003230:CDS
AAGACTTCAGAATGTCGAAA+AGG 0.556491 1_1:+5754446 Msa0003230:CDS
GGAGCTTGCAGGAGGAGTGA+TGG 0.557814 1_1:+5754592 Msa0003230:CDS
ACTTTCTTGGAAGAATATTG+GGG 0.595084 1_1:-5752531 None:intergenic
AGGTAGAGCTAGGATTTGGG+AGG 0.607159 1_1:+5752684 Msa0003230:five_prime_UTR
AATTCAAGTTGCACTCAACA+AGG 0.612180 1_1:+5756599 Msa0003230:three_prime_UTR
ATTTATGTATTTATTTCGAG+GGG 0.636208 1_1:+5756632 Msa0003230:three_prime_UTR
GTTTGTATAGAGAATTGTGA+AGG 0.675616 1_1:+5754565 Msa0003230:intron
ATTGGAGAGTAAATCAACCA+AGG 0.682126 1_1:+5752618 Msa0003230:five_prime_UTR
GCAACATCAATGGATTCACA+GGG 0.701218 1_1:+5754405 Msa0003230:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATAGAATTATGAAAT+TGG + chr1_1:5752804-5752823 Msa0003230:intron 10.0%
!! ATATTAAAGTATATTAAAGA+AGG + chr1_1:5753677-5753696 Msa0003230:intron 10.0%
!! TATGATATTAATTATTGAAA+TGG - chr1_1:5754774-5754793 None:intergenic 10.0%
!! ATAATTTATCAAATGAATGA+GGG - chr1_1:5752791-5752810 None:intergenic 15.0%
!! TATAATTTATCAAATGAATG+AGG - chr1_1:5752792-5752811 None:intergenic 15.0%
!! TATAGTCTATAATTATTAAG+AGG - chr1_1:5753277-5753296 None:intergenic 15.0%
!! AATAAAGAAATCAACATAAT+GGG - chr1_1:5753842-5753861 None:intergenic 15.0%
!! TAATAAAGAAATCAACATAA+TGG - chr1_1:5753843-5753862 None:intergenic 15.0%
!!! ATTATGTTGATTTCTTTATT+AGG + chr1_1:5753842-5753861 Msa0003230:intron 15.0%
!! TAAAAAAAATCAAGTATGAA+AGG - chr1_1:5754514-5754533 None:intergenic 15.0%
!! ATGAATAAGTGTTTATAAAT+GGG - chr1_1:5755072-5755091 None:intergenic 15.0%
!!! TGATTTTCAATTACAATTAA+CGG + chr1_1:5755479-5755498 Msa0003230:intron 15.0%
!! TTCATTTCATATCTTTAATT+TGG - chr1_1:5755932-5755951 None:intergenic 15.0%
!!! AAACTATTTTTGATAGAAAT+GGG - chr1_1:5755957-5755976 None:intergenic 15.0%
!!! TAAACTATTTTTGATAGAAA+TGG - chr1_1:5755958-5755977 None:intergenic 15.0%
!! AATATTACAACATTATAGTA+GGG - chr1_1:5756267-5756286 None:intergenic 15.0%
!! AAAAATTAGAAAGAAAATGA+AGG - chr1_1:5756314-5756333 None:intergenic 15.0%
!!! ATTTTCTTTCTAATTTTTGT+AGG + chr1_1:5756316-5756335 Msa0003230:intron 15.0%
!! TTATTTATGTATTTATTTCG+AGG + chr1_1:5756630-5756649 Msa0003230:three_prime_UTR 15.0%
!! TATTTATGTATTTATTTCGA+GGG + chr1_1:5756631-5756650 Msa0003230:three_prime_UTR 15.0%
!! AGCTTATTTAATTGTCTAAT+GGG - chr1_1:5752587-5752606 None:intergenic 20.0%
!! AAGCTTATTTAATTGTCTAA+TGG - chr1_1:5752588-5752607 None:intergenic 20.0%
!! AGAATTATGAAATTGGAATT+TGG + chr1_1:5752811-5752830 Msa0003230:intron 20.0%
!! GAATTATGAAATTGGAATTT+GGG + chr1_1:5752812-5752831 Msa0003230:intron 20.0%
!! TTAGTAATATTCATATCTAG+TGG - chr1_1:5753375-5753394 None:intergenic 20.0%
!! TACTTGATAAAAATCTTACA+CGG - chr1_1:5754026-5754045 None:intergenic 20.0%
!! AAAATCTCTAAACAAATTTG+TGG + chr1_1:5754076-5754095 Msa0003230:intron 20.0%
!! AAGATATCAGGAAAAAAATT+AGG + chr1_1:5754262-5754281 Msa0003230:intron 20.0%
!! TGAATTCATGCAATTAATTA+AGG + chr1_1:5754670-5754689 Msa0003230:CDS 20.0%
!!! TAGTTGTTACACAATAATTT+TGG - chr1_1:5754809-5754828 None:intergenic 20.0%
!! AATTATTGTGTAACAACTAT+TGG + chr1_1:5754810-5754829 Msa0003230:intron 20.0%
!! TGAATAAGTGTTTATAAATG+GGG - chr1_1:5755071-5755090 None:intergenic 20.0%
!! CATGAATAAGTGTTTATAAA+TGG - chr1_1:5755073-5755092 None:intergenic 20.0%
!! TTCAATATATAAACTATGCT+TGG + chr1_1:5755121-5755140 Msa0003230:intron 20.0%
!! ACTCAATTACACAAATTTAA+AGG - chr1_1:5755590-5755609 None:intergenic 20.0%
!! GAATATTACAACATTATAGT+AGG - chr1_1:5756268-5756287 None:intergenic 20.0%
!! AAATGAGATATGTTTCAAAA+AGG + chr1_1:5756424-5756443 Msa0003230:CDS 20.0%
!!! ATTTAAGAAGCAAAATTTTC+AGG - chr1_1:5756513-5756532 None:intergenic 20.0%
!! ATTTATGTATTTATTTCGAG+GGG + chr1_1:5756632-5756651 Msa0003230:three_prime_UTR 20.0%
! ATAGAGTAAATAAGGAACAT+TGG + chr1_1:5752709-5752728 Msa0003230:five_prime_UTR 25.0%
!! ATAATATGATGATGTCCAAA+TGG - chr1_1:5753136-5753155 None:intergenic 25.0%
!!! TAGTTGATGTTTTTTATCAG+TGG + chr1_1:5753295-5753314 Msa0003230:intron 25.0%
! TTTATCAGTGGAATGAATAT+TGG + chr1_1:5753307-5753326 Msa0003230:intron 25.0%
!! CTAGTAGAAGATATTGATTA+AGG + chr1_1:5753398-5753417 Msa0003230:intron 25.0%
! ATTGTTATAGCATACTACAT+TGG + chr1_1:5753575-5753594 Msa0003230:intron 25.0%
!! ATTGGCAGTACAAATTATTT+TGG + chr1_1:5753593-5753612 Msa0003230:intron 25.0%
!! ATTTCCCTTTTTGTAAAATG+TGG + chr1_1:5753941-5753960 Msa0003230:intron 25.0%
!! TTTTCTAAGCAGTAAACTAA+TGG - chr1_1:5754148-5754167 None:intergenic 25.0%
! AATCTTGCCAAAATATTGTA+GGG - chr1_1:5754310-5754329 None:intergenic 25.0%
! TAATCTTGCCAAAATATTGT+AGG - chr1_1:5754311-5754330 None:intergenic 25.0%
!! TTTTTATTACAAGATTGCAC+TGG + chr1_1:5754723-5754742 Msa0003230:intron 25.0%
! TATATAACTATAGATGCAAG+TGG + chr1_1:5754746-5754765 Msa0003230:intron 25.0%
! GAATAAGTGTTTATAAATGG+GGG - chr1_1:5755070-5755089 None:intergenic 25.0%
! TATGTTGCATACAAATGAAA+GGG - chr1_1:5755181-5755200 None:intergenic 25.0%
!!! CTATTTCACATAACTGATTT+TGG + chr1_1:5755274-5755293 Msa0003230:intron 25.0%
! GACTATTAGCACTTAAAAAA+TGG + chr1_1:5755619-5755638 Msa0003230:intron 25.0%
! TTCAAATACCAAACTTACAA+AGG - chr1_1:5755753-5755772 None:intergenic 25.0%
! TAATGAACATTAGATTTCTC+AGG - chr1_1:5755777-5755796 None:intergenic 25.0%
! CTATCAAAAATAGTTTACCT+TGG + chr1_1:5755961-5755980 Msa0003230:intron 25.0%
! AAACAACTAAGTACATGTTT+GGG + chr1_1:5755988-5756007 Msa0003230:intron 25.0%
! AACACAAATAATTCTTCTAG+AGG - chr1_1:5756068-5756087 None:intergenic 25.0%
! TATATACTCCAATAACTAAC+AGG - chr1_1:5756129-5756148 None:intergenic 25.0%
!!! ATATCTCATTTGCTATTCTT+GGG - chr1_1:5756415-5756434 None:intergenic 25.0%
! TGAAAATTCAGTCTGTAATT+TGG + chr1_1:5756555-5756574 Msa0003230:three_prime_UTR 25.0%
!! TAATCTGTCTTTTACATGTT+TGG - chr1_1:5756695-5756714 None:intergenic 25.0%
!! AAAGACAGATTATGAAGTTT+TGG + chr1_1:5756703-5756722 Msa0003230:three_prime_UTR 25.0%
TTGTCAATTGTGAACTATCT+CGG + chr1_1:5752475-5752494 Msa0003230:five_prime_UTR 30.0%
ACTTTCTTGGAAGAATATTG+GGG - chr1_1:5752534-5752553 None:intergenic 30.0%
GACTTTCTTGGAAGAATATT+GGG - chr1_1:5752535-5752554 None:intergenic 30.0%
TGACTTTCTTGGAAGAATAT+TGG - chr1_1:5752536-5752555 None:intergenic 30.0%
TCAAATGCATATGACTTTCT+TGG - chr1_1:5752547-5752566 None:intergenic 30.0%
ATAAGCTTAGTGAAAGTGAT+TGG + chr1_1:5752600-5752619 Msa0003230:five_prime_UTR 30.0%
GAGAAAGACTATTAAGGTTA+GGG + chr1_1:5752640-5752659 Msa0003230:five_prime_UTR 30.0%
! GTTAGGGTTCTTTGAAAATT+GGG + chr1_1:5752656-5752675 Msa0003230:five_prime_UTR 30.0%
! TTAGGGTTCTTTGAAAATTG+GGG + chr1_1:5752657-5752676 Msa0003230:five_prime_UTR 30.0%
! TCTTTGAAAATTGGGGAAAA+AGG + chr1_1:5752664-5752683 Msa0003230:five_prime_UTR 30.0%
TTGATACTAAGGATGAAAGT+TGG - chr1_1:5752747-5752766 None:intergenic 30.0%
AACTTTCATCCTTAGTATCA+AGG + chr1_1:5752746-5752765 Msa0003230:five_prime_UTR 30.0%
! AAAGCATTCAAATACATCGA+AGG - chr1_1:5753011-5753030 None:intergenic 30.0%
CAGATTAAGATTGCACATTA+AGG + chr1_1:5753102-5753121 Msa0003230:intron 30.0%
ATTAAGGTCATAACTCCATT+TGG + chr1_1:5753118-5753137 Msa0003230:intron 30.0%
AAGTGAATAAGAATGAAGTC+TGG + chr1_1:5753171-5753190 Msa0003230:intron 30.0%
CAACAGATTGTATCGAAAAT+AGG + chr1_1:5753228-5753247 Msa0003230:intron 30.0%
AAATAGGATAGAACATGCAA+TGG + chr1_1:5753244-5753263 Msa0003230:intron 30.0%
TAGGTAGTGTTTAGATAATG+TGG + chr1_1:5753444-5753463 Msa0003230:intron 30.0%
AGGTAGTGTTTAGATAATGT+GGG + chr1_1:5753445-5753464 Msa0003230:intron 30.0%
CAATAATAAAGCTTTGCAAG+TGG - chr1_1:5753559-5753578 None:intergenic 30.0%
! ACTAGAAATTGATTCCAACT+TGG + chr1_1:5753763-5753782 Msa0003230:intron 30.0%
ATAAACATAGAATGCCAAGT+TGG - chr1_1:5753780-5753799 None:intergenic 30.0%
ATTCTATGTTTATCCCGATT+TGG + chr1_1:5753787-5753806 Msa0003230:intron 30.0%
TTCTATGTTTATCCCGATTT+GGG + chr1_1:5753788-5753807 Msa0003230:intron 30.0%
!! TAAGCTATTTTGCTGTCAAT+CGG + chr1_1:5753867-5753886 Msa0003230:intron 30.0%
! TCAGCCACATTTTACAAAAA+GGG - chr1_1:5753948-5753967 None:intergenic 30.0%
! ATCAGCCACATTTTACAAAA+AGG - chr1_1:5753949-5753968 None:intergenic 30.0%
TTGATAAAAATCTTACACGG+CGG - chr1_1:5754023-5754042 None:intergenic 30.0%
! AACTAATGGTGTGTTTGTTT+TGG - chr1_1:5754134-5754153 None:intergenic 30.0%
GTTTGTATAGAGAATTGTGA+AGG + chr1_1:5754565-5754584 Msa0003230:intron 30.0%
! ATAGAGAATTGTGAAGGTTT+TGG + chr1_1:5754571-5754590 Msa0003230:intron 30.0%
! TTTTGGACAAGATCTTTTCT+AGG - chr1_1:5754641-5754660 None:intergenic 30.0%
! TGAATTCAAGACAGTGATTT+TGG - chr1_1:5754658-5754677 None:intergenic 30.0%
AATTATTGAGAGACAAAGCA+AGG - chr1_1:5754700-5754719 None:intergenic 30.0%
CTAACTCATCCTTACAAAAT+CGG - chr1_1:5755012-5755031 None:intergenic 30.0%
ATTGAAATGTCTACGTACAT+CGG - chr1_1:5755107-5755126 None:intergenic 30.0%
ATGGGAATCACCATAAAAAA+GGG - chr1_1:5755156-5755175 None:intergenic 30.0%
TATGGGAATCACCATAAAAA+AGG - chr1_1:5755157-5755176 None:intergenic 30.0%
CATACAAATGAAAGGGTTAT+GGG - chr1_1:5755174-5755193 None:intergenic 30.0%
GTATGTTGCATACAAATGAA+AGG - chr1_1:5755182-5755201 None:intergenic 30.0%
TGAAATAGCAAATACTGTCA+CGG - chr1_1:5755262-5755281 None:intergenic 30.0%
ATGTAATCAATAGCAGATTG+CGG - chr1_1:5755311-5755330 None:intergenic 30.0%
AACAGATAGAATTGTGATCA+AGG - chr1_1:5755460-5755479 None:intergenic 30.0%
TACCTAAAAAGAGGATAAAG+CGG - chr1_1:5755513-5755532 None:intergenic 30.0%
CAGATTTGATACCTAAAAAG+AGG - chr1_1:5755522-5755541 None:intergenic 30.0%
TATCACAACCAAACTTACAA+AGG - chr1_1:5755722-5755741 None:intergenic 30.0%
AAACAACACTAGTTATGTGA+TGG + chr1_1:5755876-5755895 Msa0003230:intron 30.0%
CAAACATGTACTTAGTTGTT+TGG - chr1_1:5755989-5756008 None:intergenic 30.0%
CAAACAACTAAGTACATGTT+TGG + chr1_1:5755987-5756006 Msa0003230:intron 30.0%
TAAGTACATGTTTGGGTAAT+TGG + chr1_1:5755995-5756014 Msa0003230:intron 30.0%
ACTAAACCTGTTAGCAATTA+GGG - chr1_1:5756197-5756216 None:intergenic 30.0%
AACTAAACCTGTTAGCAATT+AGG - chr1_1:5756198-5756217 None:intergenic 30.0%
CTAACAGGTTTAGTTATTCA+GGG + chr1_1:5756203-5756222 Msa0003230:intron 30.0%
! CCATAATCACACTTTTCAAA+TGG - chr1_1:5756392-5756411 None:intergenic 30.0%
! CCATTTGAAAAGTGTGATTA+TGG + chr1_1:5756389-5756408 Msa0003230:CDS 30.0%
!! CATATCTCATTTGCTATTCT+TGG - chr1_1:5756416-5756435 None:intergenic 30.0%
TGAGATATGTTTCAAAAAGG+TGG + chr1_1:5756427-5756446 Msa0003230:CDS 30.0%
TATTGGGGAAAAAGCTATGA+TGG - chr1_1:5752519-5752538 None:intergenic 35.0%
ATTGGAGAGTAAATCAACCA+AGG + chr1_1:5752618-5752637 Msa0003230:five_prime_UTR 35.0%
ACCAAGGAGAAAGACTATTA+AGG + chr1_1:5752634-5752653 Msa0003230:five_prime_UTR 35.0%
ACCTTAATAGTCTTTCTCCT+TGG - chr1_1:5752638-5752657 None:intergenic 35.0%
GGAGAAAGACTATTAAGGTT+AGG + chr1_1:5752639-5752658 Msa0003230:five_prime_UTR 35.0%
! GGTTAGGGTTCTTTGAAAAT+TGG + chr1_1:5752655-5752674 Msa0003230:five_prime_UTR 35.0%
AAAAAGGTAGAGCTAGGATT+TGG + chr1_1:5752680-5752699 Msa0003230:five_prime_UTR 35.0%
AAAAGGTAGAGCTAGGATTT+GGG + chr1_1:5752681-5752700 Msa0003230:five_prime_UTR 35.0%
GGGAGGAAATAGAGTAAATA+AGG + chr1_1:5752701-5752720 Msa0003230:five_prime_UTR 35.0%
TTTCATCCTTAGTATCAAGG+TGG + chr1_1:5752749-5752768 Msa0003230:intron 35.0%
TTCATCCTTAGTATCAAGGT+GGG + chr1_1:5752750-5752769 Msa0003230:intron 35.0%
ATCAAATGAATGAGGGATGA+AGG - chr1_1:5752784-5752803 None:intergenic 35.0%
ATGTGCAATCTTAATCTGCA+AGG - chr1_1:5753100-5753119 None:intergenic 35.0%
ATACAGTGTGAACCTGATAT+AGG + chr1_1:5753343-5753362 Msa0003230:intron 35.0%
TACAGTGTGAACCTGATATA+GGG + chr1_1:5753344-5753363 Msa0003230:intron 35.0%
TAGTTGCAATTTGTGCAACT+AGG + chr1_1:5753425-5753444 Msa0003230:intron 35.0%
GGTAGTGTTTAGATAATGTG+GGG + chr1_1:5753446-5753465 Msa0003230:intron 35.0%
!! TTTTGCTGTCAATCGGATAT+CGG + chr1_1:5753874-5753893 Msa0003230:intron 35.0%
! TGTCTAGAGTCTAGAATTCT+CGG + chr1_1:5753905-5753924 Msa0003230:intron 35.0%
AGTGTTGCGTAAAGATTGAT+TGG + chr1_1:5754175-5754194 Msa0003230:intron 35.0%
AAGACTTCAGAATGTCGAAA+AGG + chr1_1:5754446-5754465 Msa0003230:CDS 35.0%
ATTGGAAATTCTGCCTTCAT+TGG + chr1_1:5754828-5754847 Msa0003230:intron 35.0%
TTCATTGTGTTGGGTTTGAA+GGG + chr1_1:5754912-5754931 Msa0003230:intron 35.0%
GTCATATCTCTAAGTGATGT+GGG - chr1_1:5754949-5754968 None:intergenic 35.0%
CCAATGCTTTATAAACACAC+AGG - chr1_1:5754971-5754990 None:intergenic 35.0%
CCTGTGTGTTTATAAAGCAT+TGG + chr1_1:5754968-5754987 Msa0003230:intron 35.0%
! CGATTTTGTAAGGATGAGTT+AGG + chr1_1:5755010-5755029 Msa0003230:intron 35.0%
ATCACCATAAAAAAGGGGAA+TGG - chr1_1:5755150-5755169 None:intergenic 35.0%
TGGGAATCACCATAAAAAAG+GGG - chr1_1:5755155-5755174 None:intergenic 35.0%
GCATACAAATGAAAGGGTTA+TGG - chr1_1:5755175-5755194 None:intergenic 35.0%
GAAATAGCAAATACTGTCAC+GGG - chr1_1:5755261-5755280 None:intergenic 35.0%
! ATCCGCTTTATCCTCTTTTT+AGG + chr1_1:5755508-5755527 Msa0003230:intron 35.0%
! AAAATTGTCCTGTTTCTCCA+TGG + chr1_1:5755651-5755670 Msa0003230:intron 35.0%
AAGCATGAAAATACACACCA+TGG - chr1_1:5755671-5755690 None:intergenic 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr1_1:5755711-5755730 Msa0003230:intron 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr1_1:5755711-5755730 Msa0003230:intron 35.0%
TACTTAGTTGTTTGGATCCA+AGG - chr1_1:5755981-5756000 None:intergenic 35.0%
TAATTGGTGCAAACTACAGA+GGG + chr1_1:5756011-5756030 Msa0003230:intron 35.0%
TCTTCTGAGTGCATTGTATT+TGG + chr1_1:5756034-5756053 Msa0003230:intron 35.0%
AGTTCTGTCCTGTTAGTTAT+TGG + chr1_1:5756118-5756137 Msa0003230:intron 35.0%
GCTAACAGGTTTAGTTATTC+AGG + chr1_1:5756202-5756221 Msa0003230:intron 35.0%
! ATTTTTGTAGGACATCCAAG+TGG + chr1_1:5756328-5756347 Msa0003230:intron 35.0%
GGAATATGTCATGTCACAAT+TGG + chr1_1:5756448-5756467 Msa0003230:CDS 35.0%
!! AATTCAAGTTGCACTCAACA+AGG + chr1_1:5756599-5756618 Msa0003230:three_prime_UTR 35.0%
ATTTATTTCGAGGGGTCATT+TGG + chr1_1:5756640-5756659 Msa0003230:three_prime_UTR 35.0%
TTTATTTCGAGGGGTCATTT+GGG + chr1_1:5756641-5756660 Msa0003230:three_prime_UTR 35.0%
! GATGGTGATGTTTGAGTGTT+TGG - chr1_1:5752501-5752520 None:intergenic 40.0%
! CTGAGTTGCTTTCATGTGTA+TGG + chr1_1:5752955-5752974 Msa0003230:intron 40.0%
! AGTTGCTTTCATGTGTATGG+AGG + chr1_1:5752958-5752977 Msa0003230:intron 40.0%
CGATACAATCTGTTGCATGA+AGG - chr1_1:5753223-5753242 None:intergenic 40.0%
TAGTGGCATTACCCTATATC+AGG - chr1_1:5753358-5753377 None:intergenic 40.0%
TGGGGAAGATTAGTAAGCTA+AGG + chr1_1:5753464-5753483 Msa0003230:intron 40.0%
! CCTAAGGTTTTTCACTGTGT+CGG - chr1_1:5753497-5753516 None:intergenic 40.0%
CAGTGAAAAACCTTAGGTGT+CGG + chr1_1:5753500-5753519 Msa0003230:intron 40.0%
TACATTGTAACCGACACCTA+AGG - chr1_1:5753513-5753532 None:intergenic 40.0%
!! TTAGGTGTCGGTTACAATGT+AGG + chr1_1:5753512-5753531 Msa0003230:intron 40.0%
! AAGAAGGCATGTGATGCTAA+CGG + chr1_1:5753693-5753712 Msa0003230:intron 40.0%
TCTGCATAATCCAACTTGTG+CGG + chr1_1:5754233-5754252 Msa0003230:intron 40.0%
! TACCGCTCCCTACAATATTT+TGG + chr1_1:5754300-5754319 Msa0003230:intron 40.0%
TGCCAAAATATTGTAGGGAG+CGG - chr1_1:5754305-5754324 None:intergenic 40.0%
CACAGAGAAAGCAACATCAA+TGG + chr1_1:5754395-5754414 Msa0003230:exon 40.0%
! AGCAACATCAATGGATTCAC+AGG + chr1_1:5754404-5754423 Msa0003230:exon 40.0%
! GCAACATCAATGGATTCACA+GGG + chr1_1:5754405-5754424 Msa0003230:exon 40.0%
GTCTTTGCAATGAAGTTGTC+TGG - chr1_1:5754431-5754450 None:intergenic 40.0%
! GGAAAGTTGTGTCACTCATA+TGG - chr1_1:5754493-5754512 None:intergenic 40.0%
!!! GATCTTTTCTAGGACCAACT+GGG - chr1_1:5754631-5754650 None:intergenic 40.0%
!!! AGATCTTTTCTAGGACCAAC+TGG - chr1_1:5754632-5754651 None:intergenic 40.0%
GAAACCCAATGGACACAATT+AGG - chr1_1:5754870-5754889 None:intergenic 40.0%
!! AACACAATGAAGGTGTTGGA+GGG - chr1_1:5754903-5754922 None:intergenic 40.0%
TCCAACACCTTCATTGTGTT+GGG + chr1_1:5754903-5754922 Msa0003230:intron 40.0%
CTTCAAACCCAACACAATGA+AGG - chr1_1:5754913-5754932 None:intergenic 40.0%
CTTCATTGTGTTGGGTTTGA+AGG + chr1_1:5754911-5754930 Msa0003230:intron 40.0%
TCATTGTGTTGGGTTTGAAG+GGG + chr1_1:5754913-5754932 Msa0003230:intron 40.0%
GGTCATATCTCTAAGTGATG+TGG - chr1_1:5754950-5754969 None:intergenic 40.0%
!! AAAATCGGCTTGTAAGGTGA+GGG - chr1_1:5754997-5755016 None:intergenic 40.0%
!! CCTTACAAAATCGGCTTGTA+AGG - chr1_1:5755003-5755022 None:intergenic 40.0%
!! CCTTACAAGCCGATTTTGTA+AGG + chr1_1:5755000-5755019 Msa0003230:intron 40.0%
ACCATAAAAAAGGGGAATGG+AGG - chr1_1:5755147-5755166 None:intergenic 40.0%
! AAATGGTGGAAACCACATTG+CGG - chr1_1:5755237-5755256 None:intergenic 40.0%
CAAATACTGTCACGGGAAAA+TGG - chr1_1:5755254-5755273 None:intergenic 40.0%
AACACGATCTTGATCAGAGT+AGG + chr1_1:5755380-5755399 Msa0003230:intron 40.0%
AATACACACCATGGAGAAAC+AGG - chr1_1:5755662-5755681 None:intergenic 40.0%
TGGCACATTCAATCTGTCTT+GGG - chr1_1:5755845-5755864 None:intergenic 40.0%
GTAATTGGTGCAAACTACAG+AGG + chr1_1:5756010-5756029 Msa0003230:intron 40.0%
GTTTCTCCCTAATTGCTAAC+AGG + chr1_1:5756188-5756207 Msa0003230:intron 40.0%
TGCAACTTGAATTGCTTGCA+GGG - chr1_1:5756592-5756611 None:intergenic 40.0%
TTGGGGAAAAAGGTAGAGCT+AGG + chr1_1:5752674-5752693 Msa0003230:five_prime_UTR 45.0%
AGGAACCCACCTTGATACTA+AGG - chr1_1:5752758-5752777 None:intergenic 45.0%
TGAATGAGGGATGAAGGAAG+AGG - chr1_1:5752778-5752797 None:intergenic 45.0%
CCGACACAGTGAAAAACCTT+AGG + chr1_1:5753494-5753513 Msa0003230:intron 45.0%
TTACAATGTAGGCAGGCAAG+TGG + chr1_1:5753523-5753542 Msa0003230:intron 45.0%
TACAATGTAGGCAGGCAAGT+GGG + chr1_1:5753524-5753543 Msa0003230:intron 45.0%
TTACCGACACCCGAGTAATT+TGG + chr1_1:5753617-5753636 Msa0003230:intron 45.0%
ACTGCGTCTGCCAAATTACT+CGG - chr1_1:5753630-5753649 None:intergenic 45.0%
TCACAGTGAGTTCCCAAATC+GGG - chr1_1:5753803-5753822 None:intergenic 45.0%
CTCACAGTGAGTTCCCAAAT+CGG - chr1_1:5753804-5753823 None:intergenic 45.0%
! TCTTTAAGCACCGCACAAGT+TGG - chr1_1:5754246-5754265 None:intergenic 45.0%
GTGCGGTGCTTAAAGATATC+AGG + chr1_1:5754250-5754269 Msa0003230:intron 45.0%
AGTCGCCTAATTGTGTCCAT+TGG + chr1_1:5754862-5754881 Msa0003230:intron 45.0%
GTCGCCTAATTGTGTCCATT+GGG + chr1_1:5754863-5754882 Msa0003230:intron 45.0%
GTGCAATGAAGGAAACCCAA+TGG - chr1_1:5754881-5754900 None:intergenic 45.0%
!! CAACACAATGAAGGTGTTGG+AGG - chr1_1:5754904-5754923 None:intergenic 45.0%
CTCCAACACCTTCATTGTGT+TGG + chr1_1:5754902-5754921 Msa0003230:intron 45.0%
! ACCCAACACAATGAAGGTGT+TGG - chr1_1:5754907-5754926 None:intergenic 45.0%
!! CAAAATCGGCTTGTAAGGTG+AGG - chr1_1:5754998-5755017 None:intergenic 45.0%
AGTTAGGCCCCGATATAAAC+CGG + chr1_1:5755026-5755045 Msa0003230:intron 45.0%
! TCACAAGCCGGTTTATATCG+GGG - chr1_1:5755036-5755055 None:intergenic 45.0%
! CTCACAAGCCGGTTTATATC+GGG - chr1_1:5755037-5755056 None:intergenic 45.0%
! CCTCACAAGCCGGTTTATAT+CGG - chr1_1:5755038-5755057 None:intergenic 45.0%
! TACGTACATCGGTTGAGAGA+TGG - chr1_1:5755096-5755115 None:intergenic 45.0%
!!! GCCTCCATTCCCCTTTTTTA+TGG + chr1_1:5755143-5755162 Msa0003230:intron 45.0%
GACTTCTTTCAACCGCAATG+TGG + chr1_1:5755222-5755241 Msa0003230:intron 45.0%
ATACTGTCACGGGAAAATGG+TGG - chr1_1:5755251-5755270 None:intergenic 45.0%
CTTGATCAGAGTAGGTGAGT+AGG + chr1_1:5755388-5755407 Msa0003230:intron 45.0%
GTGGCACATTCAATCTGTCT+TGG - chr1_1:5755846-5755865 None:intergenic 45.0%
!! GTGTTGTTTGAGCAGTACTG+TGG - chr1_1:5755865-5755884 None:intergenic 45.0%
TATACGTGTGTGTGTGTGTG+TGG + chr1_1:5756145-5756164 Msa0003230:intron 45.0%
! GTGTGTGGTTTTCGATGACA+TGG + chr1_1:5756160-5756179 Msa0003230:intron 45.0%
GTGCAACTTGAATTGCTTGC+AGG - chr1_1:5756593-5756612 None:intergenic 45.0%
AGGTAGAGCTAGGATTTGGG+AGG + chr1_1:5752684-5752703 Msa0003230:five_prime_UTR 50.0%
!! GTGTCGGTTACAATGTAGGC+AGG + chr1_1:5753516-5753535 Msa0003230:intron 50.0%
CTGCCAAATTACTCGGGTGT+CGG - chr1_1:5753623-5753642 None:intergenic 50.0%
CTGCGTCTGCCAAATTACTC+GGG - chr1_1:5753629-5753648 None:intergenic 50.0%
!!! GTGAAGGTTTTGGAGCTTGC+AGG + chr1_1:5754581-5754600 Msa0003230:CDS 50.0%
!!! AAGGTTTTGGAGCTTGCAGG+AGG + chr1_1:5754584-5754603 Msa0003230:CDS 50.0%
CACAATTAGGCGACTAGAGG+CGG - chr1_1:5754857-5754876 None:intergenic 50.0%
GGACACAATTAGGCGACTAG+AGG - chr1_1:5754860-5754879 None:intergenic 50.0%
TTGTGTTGGGTTTGAAGGGG+TGG + chr1_1:5754916-5754935 Msa0003230:intron 50.0%
!! CCGATATAAACCGGCTTGTG+AGG + chr1_1:5755035-5755054 Msa0003230:intron 50.0%
!! ATAAACCGGCTTGTGAGGTG+AGG + chr1_1:5755040-5755059 Msa0003230:intron 50.0%
TTTCATCACGCAACGCCACT+TGG - chr1_1:5756346-5756365 None:intergenic 50.0%
CTAGAGGCGGAGTCCAATGA+AGG - chr1_1:5754844-5754863 None:intergenic 55.0%
! GGTGTTGGAGGGTGCAATGA+AGG - chr1_1:5754892-5754911 None:intergenic 55.0%
GCAATCCTCACCTCACAAGC+CGG - chr1_1:5755048-5755067 None:intergenic 55.0%
GGAGCTTGCAGGAGGAGTGA+TGG + chr1_1:5754592-5754611 Msa0003230:CDS 60.0%
GACGAGCTTGCAAGCCCAGT+TGG + chr1_1:5754614-5754633 Msa0003230:CDS 60.0%
GTAGGCAGGCAAGTGGGACG+CGG + chr1_1:5753530-5753549 Msa0003230:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 5752455 5756792 5752455 ID=Msa0003230;Name=Msa0003230
chr1_1 mRNA 5752455 5756792 5752455 ID=Msa0003230-mRNA-1;Parent=Msa0003230;Name=Msa0003230-mRNA-1;_AED=0.15;_eAED=0.15;_QI=346|1|1|1|0.66|0.5|4|278|115
chr1_1 exon 5752455 5752767 5752455 ID=Msa0003230-mRNA-1:exon:1708;Parent=Msa0003230-mRNA-1
chr1_1 exon 5754381 5754467 5754381 ID=Msa0003230-mRNA-1:exon:1709;Parent=Msa0003230-mRNA-1
chr1_1 exon 5754575 5754691 5754575 ID=Msa0003230-mRNA-1:exon:1710;Parent=Msa0003230-mRNA-1
chr1_1 exon 5756338 5756792 5756338 ID=Msa0003230-mRNA-1:exon:1711;Parent=Msa0003230-mRNA-1
chr1_1 five_prime_UTR 5752455 5752767 5752455 ID=Msa0003230-mRNA-1:five_prime_utr;Parent=Msa0003230-mRNA-1
chr1_1 five_prime_UTR 5754381 5754413 5754381 ID=Msa0003230-mRNA-1:five_prime_utr;Parent=Msa0003230-mRNA-1
chr1_1 CDS 5754414 5754467 5754414 ID=Msa0003230-mRNA-1:cds;Parent=Msa0003230-mRNA-1
chr1_1 CDS 5754575 5754691 5754575 ID=Msa0003230-mRNA-1:cds;Parent=Msa0003230-mRNA-1
chr1_1 CDS 5756338 5756514 5756338 ID=Msa0003230-mRNA-1:cds;Parent=Msa0003230-mRNA-1
chr1_1 three_prime_UTR 5756515 5756792 5756515 ID=Msa0003230-mRNA-1:three_prime_utr;Parent=Msa0003230-mRNA-1
Gene Sequence

>Msa0003230

ATGGATTCACAGGGCCAGACAACTTCATTGCAAAGACTTCAGAATGTCGAAAAGAGAATTGTGAAGGTTTTGGAGCTTGCAGGAGGAGTGATGGACGAGCTTGCAAGCCCAGTTGGTCCTAGAAAAGATCTTGTCCAAAATCACTGTCTTGAATTCATGCAATTAATTAAGGACATCCAAGTGGCGTTGCGTGATGAAATCAAAAGTGCTTGTGAATATCGTCCATTTGAAAAGTGTGATTATGGCCCAAGAATAGCAAATGAGATATGTTTCAAAAAGGTGGAATATGTCATGTCACAATTGGAAGCAATGAAACAAACTATAGATGAGTATAATGCAGCAGCCTGA
Protein sequence

>Msa0003230

MDSQGQTTSLQRLQNVEKRIVKVLELAGGVMDELASPVGPRKDLVQNHCLEFMQLIKDIQVALRDEIKSACEYRPFEKCDYGPRIANEICFKKVEYVMSQLEAMKQTIDEYNAAA*