AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0160930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0160930 MtrunA17_Chr1g0174761 99.742 774 2 0 1 774 1 774 0.0 1604
Msa0160930 MtrunA17_Chr7g0258331 93.557 776 47 3 1 774 1 775 0.0 1520
Msa0160930 MtrunA17_Chr4g0033151 82.710 775 128 4 1 774 1 770 0.0 1335
Msa0160930 MtrunA17_Chr3g0087501 68.608 790 225 8 7 774 34 822 0.0 1156
Msa0160930 MtrunA17_Chr4g0010751 71.561 756 209 5 24 774 440 1194 0.0 1154
Msa0160930 MtrunA17_Chr5g0397601 68.597 777 234 4 6 774 233 1007 0.0 1147
Msa0160930 MtrunA17_Chr1g0174581 67.342 790 239 8 3 774 9 797 0.0 1134
Msa0160930 MtrunA17_Chr4g0014711 66.150 774 250 8 8 774 276 1044 0.0 1066
Msa0160930 MtrunA17_Chr8g0349231 60.385 780 291 7 6 774 231 1003 0.0 1003
Msa0160930 MtrunA17_Chr4g0028371 58.726 785 301 11 2 774 10 783 0.0 899
Msa0160930 MtrunA17_Chr4g0035961 56.051 785 271 12 2 774 10 732 0.0 863
Msa0160930 MtrunA17_Chr7g0247271 59.281 695 261 8 93 774 281 966 0.0 856
Msa0160930 MtrunA17_Chr4g0060191 53.351 776 318 14 18 774 288 1038 0.0 808
Msa0160930 MtrunA17_Chr6g0460791 52.163 763 318 13 32 774 274 1009 0.0 781
Msa0160930 MtrunA17_Chr6g0461151 48.231 763 357 13 30 774 284 1026 0.0 736
Msa0160930 MtrunA17_Chr1g0151051 48.590 780 369 13 12 774 34 798 0.0 699
Msa0160930 MtrunA17_Chr5g0447321 41.154 780 423 12 8 774 7 763 0.0 595
Msa0160930 MtrunA17_Chr4g0010641 67.692 130 40 1 644 773 1 128 4.24e-54 182
Msa0160930 MtrunA17_Chr4g0010701 50.962 104 29 1 647 750 16 97 2.43e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0160930 AT3G57880.4 91.731 774 63 1 1 774 1 773 0.0 1494
Msa0160930 AT3G57880.2 91.731 774 63 1 1 774 1 773 0.0 1494
Msa0160930 AT3G57880.3 91.731 774 63 1 1 774 1 773 0.0 1494
Msa0160930 AT3G57880.1 91.731 774 63 1 1 774 1 773 0.0 1494
Msa0160930 AT1G51570.1 88.789 776 85 2 1 774 1 776 0.0 1446
Msa0160930 AT5G12970.1 81.677 775 135 4 1 774 1 769 0.0 1317
Msa0160930 AT5G06850.1 68.662 785 228 8 7 774 11 794 0.0 1156
Msa0160930 AT4G11610.2 67.481 778 240 5 6 774 234 1007 0.0 1132
Msa0160930 AT4G11610.1 67.481 778 240 5 6 774 238 1011 0.0 1132
Msa0160930 AT4G11610.3 67.481 778 240 5 6 774 101 874 0.0 1130
Msa0160930 AT5G48060.1 65.897 780 252 8 7 774 259 1036 0.0 1103
Msa0160930 AT1G22610.1 66.203 790 246 9 1 774 245 1029 0.0 1080
Msa0160930 AT4G00700.1 59.335 782 303 6 6 774 227 1006 0.0 969
Msa0160930 AT4G00700.2 59.335 782 303 6 6 774 227 1006 0.0 969
Msa0160930 AT3G61300.1 58.869 778 291 13 4 774 217 972 0.0 946
Msa0160930 AT1G04150.1 58.228 790 289 14 8 774 241 1012 0.0 926
Msa0160930 AT4G20080.1 58.290 772 304 11 9 774 15 774 0.0 925
Msa0160930 AT1G74720.1 52.506 798 341 13 2 774 297 1081 0.0 825
Msa0160930 AT3G03680.1 52.734 768 314 15 32 774 274 1017 0.0 783
Msa0160930 AT5G17980.1 48.810 756 356 12 30 774 314 1049 0.0 748
Msa0160930 AT5G03435.1 40.798 777 403 25 7 768 6 740 8.32e-168 502
Msa0160930 AT3G61720.1 38.985 808 435 21 7 774 6 795 3.20e-167 503
Msa0160930 AT5G03435.2 43.154 577 293 17 7 574 6 556 1.22e-140 426
Msa0160930 AT5G44760.1 51.000 400 149 9 8 400 5 364 1.14e-123 379
Msa0160930 AT5G44760.1 36.496 137 38 1 540 676 364 451 6.84e-20 94.0
Msa0160930 AT1G03370.2 28.980 245 155 11 41 281 3 232 9.07e-13 72.4
Msa0160930 AT1G03370.1 28.980 245 155 11 41 281 3 232 9.83e-13 72.4

Find 224 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTCCTTTGCAGTTTA+TGG 0.027359 1_4:+34741048 Msa0160930:CDS
TCATATCTTGTTTATAATAT+TGG 0.175025 1_4:+34742008 Msa0160930:CDS
ACCTCTTCCTGATTGGAATT+TGG 0.179060 1_4:+34742070 Msa0160930:CDS
TGAATCAGAACTGTTGTAAT+TGG 0.188345 1_4:-34741988 None:intergenic
TGCGGCGCCAAAGGACTATC+TGG 0.234328 1_4:-34740593 None:intergenic
GCTTATTGTGTAGCAAAATA+TGG 0.251693 1_4:+34741361 Msa0160930:CDS
GGCTAATTGCTGCGGGAAAA+TGG 0.272171 1_4:+34741938 Msa0160930:CDS
GCATCTGAGTGCCAAGCTTC+TGG 0.277323 1_4:-34740707 None:intergenic
CCCAAGTGGTGTGAAGAAAA+TGG 0.290205 1_4:+34741630 Msa0160930:CDS
CTCCTAAGCTTTGGTATTTG+AGG 0.293908 1_4:+34740786 Msa0160930:CDS
GCCAAACAGCTCTGGAAATC+TGG 0.301858 1_4:+34741256 Msa0160930:CDS
CACGGCCTTCTGACATTGTT+CGG 0.307253 1_4:+34742202 Msa0160930:CDS
CGGTGCCGCTTAATTTCTTC+AGG 0.307382 1_4:+34742466 Msa0160930:CDS
AGAAGGTGGTTATCATGTTT+TGG 0.311177 1_4:+34741195 Msa0160930:CDS
CACATGAGGGTTTGTCTAGA+AGG 0.326790 1_4:+34741178 Msa0160930:CDS
TAGAAAGGGTGATAAGGTTA+AGG 0.328443 1_4:+34740634 Msa0160930:CDS
CCCAATACTTCTTCTTTGTT+CGG 0.334931 1_4:-34741019 None:intergenic
TTGGAATTTGGCACTATAGA+TGG 0.341735 1_4:+34742082 Msa0160930:CDS
TTTCTCGAAGGACCGGCTTC+AGG 0.344206 1_4:+34740469 Msa0160930:CDS
GTGCCTTGAAAGAAAGCTTT+TGG 0.352266 1_4:+34739646 Msa0160930:five_prime_UTR
GAAGGACCGGCTTCAGGCCT+CGG 0.355700 1_4:+34740475 Msa0160930:CDS
AAACCAAAAGCTTTCTTTCA+AGG 0.356098 1_4:-34739649 None:intergenic
TCCAGAGCTGTTTGGCCGTT+GGG 0.357398 1_4:-34741249 None:intergenic
CCGAACAAAGAAGAAGTATT+GGG 0.371136 1_4:+34741019 Msa0160930:CDS
TTCCTTTACCTCTTCCTGAT+TGG 0.374360 1_4:+34742063 Msa0160930:CDS
CCGATGCTCTTGCAAATATT+AGG 0.375111 1_4:+34740747 Msa0160930:CDS
CCTTTATGTGAGGGTTGTTA+AGG 0.377947 1_4:+34740316 Msa0160930:CDS
GTTAAGCTCGGGAACTATAA+AGG 0.383225 1_4:+34740392 Msa0160930:CDS
CTTTGGCGCCGCAATGGTAT+AGG 0.384411 1_4:+34740603 Msa0160930:CDS
TTTATTTATCTCCTAAGCTT+TGG 0.384714 1_4:+34740777 Msa0160930:CDS
TTTGCACCTCCAGCTTTGTC+AGG 0.385021 1_4:-34741517 None:intergenic
TCCTAAGCTTTGGTATTTGA+GGG 0.385923 1_4:+34740787 Msa0160930:CDS
GGCTAATTTCTTCAGAATCA+TGG 0.388398 1_4:+34741903 Msa0160930:CDS
TCAGATGCCGCGACTGTTAG+TGG 0.391111 1_4:+34740722 Msa0160930:CDS
CCGAGCTTAACTTCGGCATA+AGG 0.395229 1_4:-34740380 None:intergenic
CAGCTCTGGAAATCTGGAAT+TGG 0.397726 1_4:+34741262 Msa0160930:CDS
ACAAACAGTGCCGTTGCTCT+TGG 0.398892 1_4:-34742330 None:intergenic
GAGAGCCGAGTGTCCATGTT+AGG 0.399650 1_4:-34742120 None:intergenic
GGTTTGATCAAATTTGCAAT+TGG 0.408748 1_4:+34741959 Msa0160930:CDS
GAGAGTTAATGCTGGCTGTT+TGG 0.410843 1_4:+34740657 Msa0160930:CDS
CCTTAACAACCCTCACATAA+AGG 0.412120 1_4:-34740316 None:intergenic
GCCCAACGGCCAAACAGCTC+TGG 0.415222 1_4:+34741248 Msa0160930:CDS
GGAATTGGTGTTCTTGAAGT+TGG 0.415516 1_4:+34741277 Msa0160930:CDS
TTTGAATGAGGTTCCGAAAC+GGG 0.416032 1_4:+34740565 Msa0160930:CDS
GGGTGTATATAGTGCATCTT+TGG 0.421466 1_4:-34741730 None:intergenic
TTGCCTTCAAAGGATGTTAC+CGG 0.422723 1_4:+34740347 Msa0160930:CDS
TCTTTCAACAGAAAATCTTC+AGG 0.424219 1_4:-34740212 None:intergenic
AACTCTGAAATCAGAGATCT+AGG 0.426167 1_4:+34739674 Msa0160930:five_prime_UTR
AACCAATCCTGAATGGAATC+AGG 0.430195 1_4:+34740439 Msa0160930:CDS
AGACTTACAGCCAACTGATA+AGG 0.432910 1_4:+34740829 Msa0160930:CDS
GGCTAAGGATTTGCCTTCAA+AGG 0.432921 1_4:+34740337 Msa0160930:CDS
TGTCAGAAGGCCGTGAAGTC+GGG 0.434203 1_4:-34742194 None:intergenic
AAAATCAGCGGCTCCTCAAA+TGG 0.439031 1_4:-34740974 None:intergenic
TTCCAGAGCTGTTTGGCCGT+TGG 0.439868 1_4:-34741250 None:intergenic
TTGACACATTCCCGACTTCA+CGG 0.441800 1_4:+34742184 Msa0160930:CDS
GTATAGGTTGGAGGATAGAA+AGG 0.443612 1_4:+34740619 Msa0160930:CDS
CGCTGAGCCACCGCTGAGAA+AGG 0.446361 1_4:+34741822 Msa0160930:CDS
TCCAGATTTCCAGAGCTGTT+TGG 0.447417 1_4:-34741257 None:intergenic
TTCCTTTGCAGTTTATGGAC+AGG 0.449319 1_4:+34741053 Msa0160930:CDS
AAGAAATTAAATTTGCAAGT+AGG 0.449686 1_4:+34741152 Msa0160930:CDS
AACTCTTCATCAAGTTCATC+CGG 0.450581 1_4:-34742162 None:intergenic
ACCACAAACCGGTGAATACT+AGG 0.450690 1_4:+34741083 Msa0160930:CDS
GACTTACAGCCAACTGATAA+GGG 0.456275 1_4:+34740830 Msa0160930:CDS
GAATTGGTGTTCTTGAAGTT+GGG 0.456895 1_4:+34741278 Msa0160930:CDS
CCGGCTTCAGGCCTCGGTGT+TGG 0.457897 1_4:+34740481 Msa0160930:CDS
GCTTCAGGCCTCGGTGTTGG+AGG 0.458158 1_4:+34740484 Msa0160930:CDS
TTCATCCGGATGAGCTGAAT+CGG 0.463142 1_4:-34742148 None:intergenic
AGTTGGGATATTGAGTGCTC+AGG 0.463537 1_4:+34741294 Msa0160930:CDS
AGAAAGGGTGATAAGGTTAA+GGG 0.464699 1_4:+34740635 Msa0160930:CDS
TATAGTGCCAAATTCCAATC+AGG 0.471795 1_4:-34742077 None:intergenic
CCTTGCACTGTGATTACAAT+TGG 0.473689 1_4:+34741466 Msa0160930:CDS
AACTGATAAGGGTAGATATC+CGG 0.474666 1_4:+34740841 Msa0160930:CDS
CGAGCTGGCTAACGGTCAAC+GGG 0.475727 1_4:-34741750 None:intergenic
ATTCAAGGATTGGTAAGGTA+AGG 0.476487 1_4:+34741542 Msa0160930:CDS
GAAAATGGGTGAAATTCAAT+TGG 0.477497 1_4:+34741645 Msa0160930:CDS
ACTGTTGGTAAGATAAGTTC+AGG 0.477704 1_4:-34742039 None:intergenic
TGTGAGGGTTGTTAAGGCTA+AGG 0.478542 1_4:+34740322 Msa0160930:CDS
GGAGGATAGAAAGGGTGATA+AGG 0.479059 1_4:+34740628 Msa0160930:CDS
GCTAATTTCTTCAGAATCAT+GGG 0.479725 1_4:+34741904 Msa0160930:CDS
AACCTGATTCCATTCAGGAT+TGG 0.483996 1_4:-34740441 None:intergenic
TTCCAATCAGGAAGAGGTAA+AGG 0.484097 1_4:-34742065 None:intergenic
GGCTGTTTGGATGGGTACAC+AGG 0.485124 1_4:+34740670 Msa0160930:CDS
CTGGAGGTGCAAAAGATTCA+AGG 0.487820 1_4:+34741527 Msa0160930:CDS
CTTATTGTGTAGCAAAATAT+GGG 0.488815 1_4:+34741362 Msa0160930:CDS
ATGAAGCATTTCCAGAAGCT+TGG 0.489119 1_4:+34740696 Msa0160930:CDS
TTTGAGGGTTAATGTGATTG+AGG 0.490505 1_4:+34740802 Msa0160930:CDS
CGGTCAGAAGGGCCACAACT+TGG 0.491080 1_4:-34742398 None:intergenic
GCGGCGCCAAAGGACTATCT+GGG 0.491089 1_4:-34740592 None:intergenic
GTTGTGAAGGACGACTTCAT+TGG 0.491403 1_4:+34740527 Msa0160930:CDS
GTTGAGTATATGCTTGATGT+TGG 0.492238 1_4:+34741850 Msa0160930:CDS
GTTAATGCTGGCTGTTTGGA+TGG 0.493096 1_4:+34740661 Msa0160930:CDS
GGCTGCAGTCTTTACTGAGC+TGG 0.495480 1_4:+34742301 Msa0160930:CDS
AAAGAGACCAAACCCCATCT+TGG 0.497107 1_4:+34740230 Msa0160930:CDS
ATGTCTCAACACATAAATTC+CGG 0.498537 1_4:-34742418 None:intergenic
CTCAACAAGGTCATATGTGC+TGG 0.498939 1_4:-34740282 None:intergenic
TCCATGTTAGGAGGGTGTCT+TGG 0.501157 1_4:-34742108 None:intergenic
CAAGTTGTGGCCCTTCTGAC+CGG 0.503387 1_4:+34742399 Msa0160930:CDS
TCCGAACAAAGAAGAAGTAT+TGG 0.503767 1_4:+34741018 Msa0160930:CDS
TCACGCCGATTCAGCTCATC+CGG 0.505053 1_4:+34742143 Msa0160930:CDS
TGTTGCTGCGGAACCATTTG+AGG 0.513683 1_4:+34740961 Msa0160930:CDS
TTAGCCAGCTCGACAGTTTG+AGG 0.514684 1_4:+34741761 Msa0160930:CDS
ATGTCAGAAGGCCGTGAAGT+CGG 0.514758 1_4:-34742195 None:intergenic
AACAGAAAATCTTCAGGCGG+TGG 0.514836 1_4:-34740206 None:intergenic
AGAAGAAAACCAATCCTGAA+TGG 0.515488 1_4:+34740432 Msa0160930:CDS
ACCCTCAAATACCAAAGCTT+AGG 0.516986 1_4:-34740788 None:intergenic
GGTGCAAAAGATTCAAGGAT+TGG 0.520817 1_4:+34741532 Msa0160930:CDS
TCGAGCTGGCTAACGGTCAA+CGG 0.520943 1_4:-34741751 None:intergenic
GATCGACTTCGAAGCATAGC+TGG 0.524982 1_4:+34742234 Msa0160930:CDS
CACCACTTGGGTGAAGAACT+AGG 0.526449 1_4:-34741618 None:intergenic
AGCCGAGTGTCCATGTTAGG+AGG 0.527424 1_4:-34742117 None:intergenic
TTGCATGGTCCTGACAAAGC+TGG 0.527750 1_4:+34741508 Msa0160930:CDS
GCCAAATTCCAATCAGGAAG+AGG 0.528138 1_4:-34742071 None:intergenic
ACACATAAATTCCGGTCAGA+AGG 0.532406 1_4:-34742410 None:intergenic
AGCATGCAATCAGTTCTTGC+AGG 0.532832 1_4:-34742495 None:intergenic
CCAAGTGGTGTGAAGAAAAT+GGG 0.533679 1_4:+34741631 Msa0160930:CDS
ATTTGAATGAGGTTCCGAAA+CGG 0.535448 1_4:+34740564 Msa0160930:CDS
CAGGAGACTCGACCACAAAC+CGG 0.535543 1_4:+34741072 Msa0160930:CDS
TGCAAGTAGGATTCACATGA+GGG 0.537596 1_4:+34741165 Msa0160930:CDS
AAGAAATTAAGCGGCACCGA+CGG 0.541607 1_4:-34742462 None:intergenic
CACATAAATTCCGGTCAGAA+GGG 0.542177 1_4:-34742409 None:intergenic
ACCTAGTATTCACCGGTTTG+TGG 0.542498 1_4:-34741084 None:intergenic
TAGCAAAATATGGGCAGAAG+TGG 0.544546 1_4:+34741371 Msa0160930:CDS
CTATACCATTGCGGCGCCAA+AGG 0.545107 1_4:-34740602 None:intergenic
GGACTAGAATCTCTCAGAGC+AGG 0.546735 1_4:+34740903 Msa0160930:CDS
GTTGGGATATTGAGTGCTCA+GGG 0.547198 1_4:+34741295 Msa0160930:CDS
AGAAGGGCCACAACTTGGAA+TGG 0.547838 1_4:-34742393 None:intergenic
TATCAATCCGTTGTGGAATG+AGG 0.548822 1_4:+34740928 Msa0160930:CDS
CTGCCGGTAACATCCTTTGA+AGG 0.549728 1_4:-34740350 None:intergenic
GTAAAGAAAAGCTGAAGCAA+TGG 0.549802 1_4:-34739113 None:intergenic
GTATACATACCTTGTCAGAA+TGG 0.550693 1_4:-34739168 None:intergenic
GGCGCCGCAATGGTATAGGT+TGG 0.550817 1_4:+34740607 Msa0160930:CDS
ATAGTTCCCGAGCTTAACTT+CGG 0.551532 1_4:-34740387 None:intergenic
GTGAAGAACTAGGAGTGGAT+AGG 0.551635 1_4:-34741608 None:intergenic
GCCAAGACACCCTCCTAACA+TGG 0.551812 1_4:+34742107 Msa0160930:CDS
GGCATAAGGATCACAACTGC+CGG 0.556517 1_4:-34740366 None:intergenic
TGTGTGGTTTGATTTGAATG+AGG 0.557487 1_4:+34740553 Msa0160930:CDS
TGCCGCTTAATTTCTTCAGG+AGG 0.557905 1_4:+34742469 Msa0160930:CDS
AAAAGATTCAAGGATTGGTA+AGG 0.560457 1_4:+34741537 Msa0160930:CDS
CCTAATATTTGCAAGAGCAT+CGG 0.563372 1_4:-34740747 None:intergenic
AGACCAAACCCCATCTTGGA+GGG 0.563491 1_4:+34740234 Msa0160930:CDS
CCAACACCGAGGCCTGAAGC+CGG 0.564103 1_4:-34740481 None:intergenic
GAGACCAAACCCCATCTTGG+AGG 0.564193 1_4:+34740233 Msa0160930:CDS
ACGACTTCATTGGTCGTGTG+TGG 0.564266 1_4:+34740537 Msa0160930:CDS
TTTGATAATTGTCATTTGCA+TGG 0.564967 1_4:+34741493 Msa0160930:CDS
ATAGTCCTTTGGCGCCGCAA+TGG 0.565983 1_4:+34740597 Msa0160930:CDS
GATGCCTCAAACTGTCGAGC+TGG 0.566280 1_4:-34741765 None:intergenic
AAAACATATCATTGTAGAAG+GGG 0.567643 1_4:+34741120 Msa0160930:CDS
CCTTATGCCGAAGTTAAGCT+CGG 0.570182 1_4:+34740380 Msa0160930:CDS
GGTGGAATGAGCAATATACT+TGG 0.572552 1_4:+34741431 Msa0160930:CDS
TTGAGTGGGCTAATTGCTGC+GGG 0.575228 1_4:+34741931 Msa0160930:CDS
GGATATCTACCCTTATCAGT+TGG 0.576446 1_4:-34740839 None:intergenic
TATAGGTTGGAGGATAGAAA+GGG 0.576864 1_4:+34740620 Msa0160930:CDS
TTAAACCACCTAGTATTCAC+CGG 0.579069 1_4:-34741091 None:intergenic
CTCCTAGTTCTTCACCCAAG+TGG 0.579462 1_4:+34741616 Msa0160930:CDS
CAAACTGTCGAGCTGGCTAA+CGG 0.580833 1_4:-34741758 None:intergenic
GCCGCAATGGTATAGGTTGG+AGG 0.582372 1_4:+34740610 Msa0160930:CDS
TGTGACTCCATTCCAAGTTG+TGG 0.583105 1_4:+34742386 Msa0160930:CDS
ACCAAACCCCATCTTGGAGG+GGG 0.588344 1_4:+34740236 Msa0160930:CDS
TTGCAAGTAGGATTCACATG+AGG 0.591241 1_4:+34741164 Msa0160930:CDS
GGCAGAAGTGGATTCGAACA+AGG 0.592797 1_4:+34741383 Msa0160930:CDS
CTCCTGTCCATAAACTGCAA+AGG 0.598615 1_4:-34741055 None:intergenic
CTGGCGTGATCCAAGAGCAA+CGG 0.599242 1_4:+34742320 Msa0160930:CDS
AAGAGGTAAAGGAAAACTGT+TGG 0.606111 1_4:-34742054 None:intergenic
TTAATGCTGGCTGTTTGGAT+GGG 0.606592 1_4:+34740662 Msa0160930:CDS
CCAATTGTAATCACAGTGCA+AGG 0.607117 1_4:-34741466 None:intergenic
ACTACCTCCTTTCTCAGCGG+TGG 0.608379 1_4:-34741829 None:intergenic
ACCCTCCTAACATGGACACT+CGG 0.608381 1_4:+34742115 Msa0160930:CDS
GTTGGCGACTTAGCTACTCA+AGG 0.610145 1_4:+34742273 Msa0160930:CDS
GGTAGGATACAGACTGTGGT+TGG 0.612469 1_4:+34742255 Msa0160930:CDS
GATGCAATACCTTTATGTGA+GGG 0.612976 1_4:+34740307 Msa0160930:CDS
GACCAAACCCCATCTTGGAG+GGG 0.614269 1_4:+34740235 Msa0160930:CDS
CGGCGCCAAAGGACTATCTG+GGG 0.614676 1_4:-34740591 None:intergenic
TTGGCGACTTAGCTACTCAA+GGG 0.614848 1_4:+34742274 Msa0160930:CDS
GGAAGCTTATGACGGAACCT+CGG 0.615117 1_4:-34742441 None:intergenic
CTCATCCGAACAATGTCAGA+AGG 0.615248 1_4:-34742207 None:intergenic
GGAGGTTACTGTGAAAGATA+AGG 0.615500 1_4:+34740502 Msa0160930:CDS
CTTATGCCGAAGTTAAGCTC+GGG 0.616554 1_4:+34740381 Msa0160930:CDS
TTGATGTTGGTTCTCACATG+TGG 0.616570 1_4:+34741863 Msa0160930:CDS
TTCAACAGAAAATCTTCAGG+CGG 0.617196 1_4:-34740209 None:intergenic
GGTTAAGGGAGAGTTAATGC+TGG 0.618376 1_4:+34740649 Msa0160930:CDS
AGATGCAATACCTTTATGTG+AGG 0.619566 1_4:+34740306 Msa0160930:CDS
AGAATCATGGGAGTATTGAG+TGG 0.620422 1_4:+34741916 Msa0160930:CDS
ACTTAGCTACTCAAGGGGAA+AGG 0.621940 1_4:+34742280 Msa0160930:CDS
CAGCAGTGATCTTCGCCCAA+CGG 0.622948 1_4:+34741234 Msa0160930:CDS
GCCGAGTGTCCATGTTAGGA+GGG 0.626241 1_4:-34742116 None:intergenic
GAATCATGGGAGTATTGAGT+GGG 0.626649 1_4:+34741917 Msa0160930:CDS
GAATTTGGCACTATAGATGG+AGG 0.626699 1_4:+34742085 Msa0160930:CDS
ATTGAGTGGGCTAATTGCTG+CGG 0.627017 1_4:+34741930 Msa0160930:CDS
TTTGAGCAGCGAAAATGCAG+AGG 0.627054 1_4:+34740183 Msa0160930:intron
ATGAGGGTTTGTCTAGAAGG+TGG 0.627125 1_4:+34741181 Msa0160930:CDS
GTGGAATGAGCAATATACTT+GGG 0.627653 1_4:+34741432 Msa0160930:CDS
GGATTTAATGTTTGTTGCTG+CGG 0.629021 1_4:+34740949 Msa0160930:CDS
CTTGGGTGAAGAACTAGGAG+TGG 0.630605 1_4:-34741613 None:intergenic
TGAGCCACCGCTGAGAAAGG+AGG 0.630758 1_4:+34741825 Msa0160930:CDS
GGTATTGCATCTGCTCAACA+AGG 0.633340 1_4:-34740295 None:intergenic
TAGTGCATCTTTGGCAACAG+AGG 0.634644 1_4:-34741721 None:intergenic
GCACCGACGGAAGCTTATGA+CGG 0.638901 1_4:-34742449 None:intergenic
AACTCAGCTCCATTCTGACA+AGG 0.642851 1_4:+34739159 Msa0160930:five_prime_UTR
GCAGGAGTATCAATCCGTTG+TGG 0.643845 1_4:+34740921 Msa0160930:CDS
TTGATAGCTTTATGCCGAGG+TGG 0.644610 1_4:+34741413 Msa0160930:CDS
GAATGAGCAATATACTTGGG+AGG 0.645094 1_4:+34741435 Msa0160930:CDS
GAAAGATAAGGATGTTGTGA+AGG 0.645624 1_4:+34740514 Msa0160930:CDS
GTGGAGTATGAGAAGAAGCA+AGG 0.650662 1_4:+34741882 Msa0160930:CDS
ATCGGTTCCACTAACAGTCG+CGG 0.651678 1_4:-34740729 None:intergenic
AGCCTCCTGAAGAAATTAAG+CGG 0.651985 1_4:-34742471 None:intergenic
GTTCCGTCATAAGCTTCCGT+CGG 0.653799 1_4:+34742446 Msa0160930:CDS
GTGAAATTCAATTGGCTGTG+AGG 0.654400 1_4:+34741653 Msa0160930:CDS
GGAACTATAAAGGAACTACT+CGG 0.658774 1_4:+34740402 Msa0160930:CDS
ATTAAATCCTCATTCCACAA+CGG 0.659499 1_4:-34740935 None:intergenic
CTACTCAGATAGTTTCAATG+AGG 0.663957 1_4:+34741791 Msa0160930:CDS
CGAACAAAGAAGAAGTATTG+GGG 0.665700 1_4:+34741020 Msa0160930:CDS
TCATTGATAGCTTTATGCCG+AGG 0.666881 1_4:+34741410 Msa0160930:CDS
TCCTCCAACCTATACCATTG+CGG 0.673444 1_4:-34740611 None:intergenic
GGCGCCAAAGGACTATCTGG+GGG 0.676642 1_4:-34740590 None:intergenic
GACTTCGAAGCATAGCTGGT+AGG 0.678712 1_4:+34742238 Msa0160930:CDS
TCTTCCACACTCAAAATCAG+CGG 0.687134 1_4:-34740986 None:intergenic
TTTATGTGTTGAGACATCCG+AGG 0.696759 1_4:+34742424 Msa0160930:CDS
TGGCGACTTAGCTACTCAAG+GGG 0.698844 1_4:+34742275 Msa0160930:CDS
AGCTGGTAGGATACAGACTG+TGG 0.703110 1_4:+34742251 Msa0160930:CDS
CATGGTCCTGACAAAGCTGG+AGG 0.704809 1_4:+34741511 Msa0160930:CDS
GTATATTGCTCATTCCACCT+CGG 0.716433 1_4:-34741427 None:intergenic
TCAACTACCTCCTTTCTCAG+CGG 0.739237 1_4:-34741832 None:intergenic
CACAGTAACCTCCAACACCG+AGG 0.741552 1_4:-34740492 None:intergenic
ACAAACCGGTGAATACTAGG+TGG 0.763717 1_4:+34741086 Msa0160930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATTTTCACAAAATTTT+AGG + chr1_4:34740040-34740059 Msa0160930:intron 10.0%
!! AATAAAATGTAACATTTCAA+AGG - chr1_4:34739509-34739528 None:intergenic 15.0%
!!! TGTTTTATTGTTGTTTTTTT+AGG + chr1_4:34740098-34740117 Msa0160930:intron 15.0%
!!! TCATATCTTGTTTATAATAT+TGG + chr1_4:34742008-34742027 Msa0160930:CDS 15.0%
!!! TGTTTTTAGCAAATATTTCT+GGG + chr1_4:34739398-34739417 Msa0160930:intron 20.0%
!!! ATTTTTTGTTTATTACTTGC+AGG + chr1_4:34739606-34739625 Msa0160930:intron 20.0%
!! AAAAACATATCATTGTAGAA+GGG + chr1_4:34741119-34741138 Msa0160930:CDS 20.0%
!! AAGAAATTAAATTTGCAAGT+AGG + chr1_4:34741152-34741171 Msa0160930:CDS 20.0%
! TAATGTTGAAGAAGTGAAAA+TGG - chr1_4:34739220-34739239 None:intergenic 25.0%
!!! CTGTTTTTAGCAAATATTTC+TGG + chr1_4:34739397-34739416 Msa0160930:intron 25.0%
!!! TTCACAAAATTTTAGGTGTT+TGG + chr1_4:34740047-34740066 Msa0160930:intron 25.0%
!!! AAATTTTAGGTGTTTGGATT+TGG + chr1_4:34740053-34740072 Msa0160930:intron 25.0%
! TTTATTTATCTCCTAAGCTT+TGG + chr1_4:34740777-34740796 Msa0160930:CDS 25.0%
!!! TTTTTGTGAAGGCTATTTTA+GGG + chr1_4:34740867-34740886 Msa0160930:CDS 25.0%
! GAAAAACATATCATTGTAGA+AGG + chr1_4:34741118-34741137 Msa0160930:CDS 25.0%
! AAAACATATCATTGTAGAAG+GGG + chr1_4:34741120-34741139 Msa0160930:CDS 25.0%
! CTTATTGTGTAGCAAAATAT+GGG + chr1_4:34741362-34741381 Msa0160930:CDS 25.0%
!! TTTGATAATTGTCATTTGCA+TGG + chr1_4:34741493-34741512 Msa0160930:CDS 25.0%
AAACCAAAAGCTTTCTTTCA+AGG - chr1_4:34739652-34739671 None:intergenic 30.0%
!! TTGAAAGAAAGCTTTTGGTT+TGG + chr1_4:34739651-34739670 Msa0160930:five_prime_UTR 30.0%
!!! TTTTTTTAGGCTTCTGAAGT+TGG + chr1_4:34740111-34740130 Msa0160930:intron 30.0%
TCTTTCAACAGAAAATCTTC+AGG - chr1_4:34740215-34740234 None:intergenic 30.0%
!! GTTTTTGTGAAGGCTATTTT+AGG + chr1_4:34740866-34740885 Msa0160930:CDS 30.0%
ATTAAATCCTCATTCCACAA+CGG - chr1_4:34740938-34740957 None:intergenic 30.0%
ATGCCGATGAAAAATAAAGA+TGG + chr1_4:34741322-34741341 Msa0160930:CDS 30.0%
TGCCGATGAAAAATAAAGAT+GGG + chr1_4:34741323-34741342 Msa0160930:CDS 30.0%
!!! CTCCCATCTTTATTTTTCAT+CGG - chr1_4:34741328-34741347 None:intergenic 30.0%
GATGAAAAATAAAGATGGGA+GGG + chr1_4:34741327-34741346 Msa0160930:CDS 30.0%
ATGAAAAATAAAGATGGGAG+GGG + chr1_4:34741328-34741347 Msa0160930:CDS 30.0%
GCTTATTGTGTAGCAAAATA+TGG + chr1_4:34741361-34741380 Msa0160930:CDS 30.0%
! AAAAGATTCAAGGATTGGTA+AGG + chr1_4:34741537-34741556 Msa0160930:CDS 30.0%
GAAAATGGGTGAAATTCAAT+TGG + chr1_4:34741645-34741664 Msa0160930:CDS 30.0%
GCTAATTTCTTCAGAATCAT+GGG + chr1_4:34741904-34741923 Msa0160930:CDS 30.0%
GGTTTGATCAAATTTGCAAT+TGG + chr1_4:34741959-34741978 Msa0160930:CDS 30.0%
TGAATCAGAACTGTTGTAAT+TGG - chr1_4:34741991-34742010 None:intergenic 30.0%
ATGTCTCAACACATAAATTC+CGG - chr1_4:34742421-34742440 None:intergenic 30.0%
GTAAAGAAAAGCTGAAGCAA+TGG - chr1_4:34739116-34739135 None:intergenic 35.0%
GTATACATACCTTGTCAGAA+TGG - chr1_4:34739171-34739190 None:intergenic 35.0%
TGAAGAAGTGAAAATGGTGA+AGG - chr1_4:34739214-34739233 None:intergenic 35.0%
GTGTGACAAGTTATAGTGTT+TGG + chr1_4:34739345-34739364 Msa0160930:intron 35.0%
CTGGGAAAACTTGATGAAAT+TGG + chr1_4:34739416-34739435 Msa0160930:intron 35.0%
!! AACTCTGAAATCAGAGATCT+AGG + chr1_4:34739674-34739693 Msa0160930:five_prime_UTR 35.0%
ACACTTCAACTTCAGATTGT+AGG + chr1_4:34739867-34739886 Msa0160930:intron 35.0%
!! TTGGATTTGGTGACTTGTAA+CGG + chr1_4:34740066-34740085 Msa0160930:intron 35.0%
CAGCAACATCTTAGTTTATC+TGG + chr1_4:34740143-34740162 Msa0160930:intron 35.0%
TTCAACAGAAAATCTTCAGG+CGG - chr1_4:34740212-34740231 None:intergenic 35.0%
AGATGCAATACCTTTATGTG+AGG + chr1_4:34740306-34740325 Msa0160930:CDS 35.0%
GATGCAATACCTTTATGTGA+GGG + chr1_4:34740307-34740326 Msa0160930:CDS 35.0%
GGAACTATAAAGGAACTACT+CGG + chr1_4:34740402-34740421 Msa0160930:CDS 35.0%
AGAAGAAAACCAATCCTGAA+TGG + chr1_4:34740432-34740451 Msa0160930:CDS 35.0%
GAAAGATAAGGATGTTGTGA+AGG + chr1_4:34740514-34740533 Msa0160930:CDS 35.0%
!! TGTGTGGTTTGATTTGAATG+AGG + chr1_4:34740553-34740572 Msa0160930:CDS 35.0%
ATTTGAATGAGGTTCCGAAA+CGG + chr1_4:34740564-34740583 Msa0160930:CDS 35.0%
! TATAGGTTGGAGGATAGAAA+GGG + chr1_4:34740620-34740639 Msa0160930:CDS 35.0%
TAGAAAGGGTGATAAGGTTA+AGG + chr1_4:34740634-34740653 Msa0160930:CDS 35.0%
AGAAAGGGTGATAAGGTTAA+GGG + chr1_4:34740635-34740654 Msa0160930:CDS 35.0%
! CCTAATATTTGCAAGAGCAT+CGG - chr1_4:34740750-34740769 None:intergenic 35.0%
! TCCTAAGCTTTGGTATTTGA+GGG + chr1_4:34740787-34740806 Msa0160930:CDS 35.0%
TTTGAGGGTTAATGTGATTG+AGG + chr1_4:34740802-34740821 Msa0160930:CDS 35.0%
AACTGATAAGGGTAGATATC+CGG + chr1_4:34740841-34740860 Msa0160930:CDS 35.0%
! ATATCCGGAAGTTTTTGTGA+AGG + chr1_4:34740856-34740875 Msa0160930:CDS 35.0%
ATAGCCTTCACAAAAACTTC+CGG - chr1_4:34740863-34740882 None:intergenic 35.0%
! GGATTTAATGTTTGTTGCTG+CGG + chr1_4:34740949-34740968 Msa0160930:CDS 35.0%
! TCCGAACAAAGAAGAAGTAT+TGG + chr1_4:34741018-34741037 Msa0160930:CDS 35.0%
CCCAATACTTCTTCTTTGTT+CGG - chr1_4:34741022-34741041 None:intergenic 35.0%
!! CCGAACAAAGAAGAAGTATT+GGG + chr1_4:34741019-34741038 Msa0160930:CDS 35.0%
!! CGAACAAAGAAGAAGTATTG+GGG + chr1_4:34741020-34741039 Msa0160930:CDS 35.0%
TGCAATTCCTTTGCAGTTTA+TGG + chr1_4:34741048-34741067 Msa0160930:CDS 35.0%
TTAAACCACCTAGTATTCAC+CGG - chr1_4:34741094-34741113 None:intergenic 35.0%
! AGAAGGTGGTTATCATGTTT+TGG + chr1_4:34741195-34741214 Msa0160930:CDS 35.0%
! GAATTGGTGTTCTTGAAGTT+GGG + chr1_4:34741278-34741297 Msa0160930:CDS 35.0%
CGATGAAAAATAAAGATGGG+AGG + chr1_4:34741326-34741345 Msa0160930:CDS 35.0%
TGAAAAATAAAGATGGGAGG+GGG + chr1_4:34741329-34741348 Msa0160930:CDS 35.0%
GTGGAATGAGCAATATACTT+GGG + chr1_4:34741432-34741451 Msa0160930:CDS 35.0%
! ATTCAAGGATTGGTAAGGTA+AGG + chr1_4:34741542-34741561 Msa0160930:CDS 35.0%
CTACTCAGATAGTTTCAATG+AGG + chr1_4:34741791-34741810 Msa0160930:CDS 35.0%
GTTGAGTATATGCTTGATGT+TGG + chr1_4:34741850-34741869 Msa0160930:CDS 35.0%
GGCTAATTTCTTCAGAATCA+TGG + chr1_4:34741903-34741922 Msa0160930:CDS 35.0%
! ACTGTTGGTAAGATAAGTTC+AGG - chr1_4:34742042-34742061 None:intergenic 35.0%
AAGAGGTAAAGGAAAACTGT+TGG - chr1_4:34742057-34742076 None:intergenic 35.0%
TATAGTGCCAAATTCCAATC+AGG - chr1_4:34742080-34742099 None:intergenic 35.0%
TTGGAATTTGGCACTATAGA+TGG + chr1_4:34742082-34742101 Msa0160930:CDS 35.0%
AACTCTTCATCAAGTTCATC+CGG - chr1_4:34742165-34742184 None:intergenic 35.0%
! AATGGAGCTGAGTTCTTTTG+AGG - chr1_4:34739153-34739172 None:intergenic 40.0%
GAGTTTCTTGTGTGTGTGTT+TGG + chr1_4:34739323-34739342 Msa0160930:intron 40.0%
! GTGCCTTGAAAGAAAGCTTT+TGG + chr1_4:34739646-34739665 Msa0160930:five_prime_UTR 40.0%
AACCATGTAACCACATGTCT+CGG - chr1_4:34739927-34739946 None:intergenic 40.0%
TATGTGTCAGGTGTTTGTGT+CGG + chr1_4:34740004-34740023 Msa0160930:intron 40.0%
CCTTAACAACCCTCACATAA+AGG - chr1_4:34740319-34740338 None:intergenic 40.0%
CCTTTATGTGAGGGTTGTTA+AGG + chr1_4:34740316-34740335 Msa0160930:CDS 40.0%
TTGCCTTCAAAGGATGTTAC+CGG + chr1_4:34740347-34740366 Msa0160930:CDS 40.0%
ATAGTTCCCGAGCTTAACTT+CGG - chr1_4:34740390-34740409 None:intergenic 40.0%
GTTAAGCTCGGGAACTATAA+AGG + chr1_4:34740392-34740411 Msa0160930:CDS 40.0%
AACCAATCCTGAATGGAATC+AGG + chr1_4:34740439-34740458 Msa0160930:CDS 40.0%
AACCTGATTCCATTCAGGAT+TGG - chr1_4:34740444-34740463 None:intergenic 40.0%
GCAAAAACCTGATTCCATTC+AGG - chr1_4:34740449-34740468 None:intergenic 40.0%
! TCAGGTTTTTGCTTTCTCGA+AGG + chr1_4:34740457-34740476 Msa0160930:CDS 40.0%
! TTTTTGCTTTCTCGAAGGAC+CGG + chr1_4:34740462-34740481 Msa0160930:CDS 40.0%
GGAGGTTACTGTGAAAGATA+AGG + chr1_4:34740502-34740521 Msa0160930:CDS 40.0%
TTTGAATGAGGTTCCGAAAC+GGG + chr1_4:34740565-34740584 Msa0160930:CDS 40.0%
! GTATAGGTTGGAGGATAGAA+AGG + chr1_4:34740619-34740638 Msa0160930:CDS 40.0%
! TTAATGCTGGCTGTTTGGAT+GGG + chr1_4:34740662-34740681 Msa0160930:CDS 40.0%
! ATGAAGCATTTCCAGAAGCT+TGG + chr1_4:34740696-34740715 Msa0160930:CDS 40.0%
CCGATGCTCTTGCAAATATT+AGG + chr1_4:34740747-34740766 Msa0160930:CDS 40.0%
! CTCCTAAGCTTTGGTATTTG+AGG + chr1_4:34740786-34740805 Msa0160930:CDS 40.0%
ACCCTCAAATACCAAAGCTT+AGG - chr1_4:34740791-34740810 None:intergenic 40.0%
AGACTTACAGCCAACTGATA+AGG + chr1_4:34740829-34740848 Msa0160930:CDS 40.0%
GACTTACAGCCAACTGATAA+GGG + chr1_4:34740830-34740849 Msa0160930:CDS 40.0%
GGATATCTACCCTTATCAGT+TGG - chr1_4:34740842-34740861 None:intergenic 40.0%
!! GAAGGCTATTTTAGGGAATC+AGG + chr1_4:34740874-34740893 Msa0160930:CDS 40.0%
!! TTTTAGGGAATCAGGCATTG+AGG + chr1_4:34740882-34740901 Msa0160930:CDS 40.0%
TATCAATCCGTTGTGGAATG+AGG + chr1_4:34740928-34740947 Msa0160930:CDS 40.0%
TCTTCCACACTCAAAATCAG+CGG - chr1_4:34740989-34741008 None:intergenic 40.0%
TTCCTTTGCAGTTTATGGAC+AGG + chr1_4:34741053-34741072 Msa0160930:CDS 40.0%
TTGCAAGTAGGATTCACATG+AGG + chr1_4:34741164-34741183 Msa0160930:CDS 40.0%
TGCAAGTAGGATTCACATGA+GGG + chr1_4:34741165-34741184 Msa0160930:CDS 40.0%
! GGAATTGGTGTTCTTGAAGT+TGG + chr1_4:34741277-34741296 Msa0160930:CDS 40.0%
TAGCAAAATATGGGCAGAAG+TGG + chr1_4:34741371-34741390 Msa0160930:CDS 40.0%
!! TCATTGATAGCTTTATGCCG+AGG + chr1_4:34741410-34741429 Msa0160930:CDS 40.0%
GTATATTGCTCATTCCACCT+CGG - chr1_4:34741430-34741449 None:intergenic 40.0%
GGTGGAATGAGCAATATACT+TGG + chr1_4:34741431-34741450 Msa0160930:CDS 40.0%
GAATGAGCAATATACTTGGG+AGG + chr1_4:34741435-34741454 Msa0160930:CDS 40.0%
CCAATTGTAATCACAGTGCA+AGG - chr1_4:34741469-34741488 None:intergenic 40.0%
CCTTGCACTGTGATTACAAT+TGG + chr1_4:34741466-34741485 Msa0160930:CDS 40.0%
GGTGCAAAAGATTCAAGGAT+TGG + chr1_4:34741532-34741551 Msa0160930:CDS 40.0%
! CCATTTTCTTCACACCACTT+GGG - chr1_4:34741633-34741652 None:intergenic 40.0%
CCAAGTGGTGTGAAGAAAAT+GGG + chr1_4:34741631-34741650 Msa0160930:CDS 40.0%
GTGAAATTCAATTGGCTGTG+AGG + chr1_4:34741653-34741672 Msa0160930:CDS 40.0%
GGGTGTATATAGTGCATCTT+TGG - chr1_4:34741733-34741752 None:intergenic 40.0%
!! TTGATGTTGGTTCTCACATG+TGG + chr1_4:34741863-34741882 Msa0160930:CDS 40.0%
!! AGAATCATGGGAGTATTGAG+TGG + chr1_4:34741916-34741935 Msa0160930:CDS 40.0%
!! GAATCATGGGAGTATTGAGT+GGG + chr1_4:34741917-34741936 Msa0160930:CDS 40.0%
TTCCTTTACCTCTTCCTGAT+TGG + chr1_4:34742063-34742082 Msa0160930:CDS 40.0%
TTCCAATCAGGAAGAGGTAA+AGG - chr1_4:34742068-34742087 None:intergenic 40.0%
ACCTCTTCCTGATTGGAATT+TGG + chr1_4:34742070-34742089 Msa0160930:CDS 40.0%
GAATTTGGCACTATAGATGG+AGG + chr1_4:34742085-34742104 Msa0160930:CDS 40.0%
CACATAAATTCCGGTCAGAA+GGG - chr1_4:34742412-34742431 None:intergenic 40.0%
ACACATAAATTCCGGTCAGA+AGG - chr1_4:34742413-34742432 None:intergenic 40.0%
TTTATGTGTTGAGACATCCG+AGG + chr1_4:34742424-34742443 Msa0160930:CDS 40.0%
AGCCTCCTGAAGAAATTAAG+CGG - chr1_4:34742474-34742493 None:intergenic 40.0%
AACTCAGCTCCATTCTGACA+AGG + chr1_4:34739159-34739178 Msa0160930:five_prime_UTR 45.0%
TACCGAGACATGTGGTTACA+TGG + chr1_4:34739922-34739941 Msa0160930:intron 45.0%
GGTGTCTACATGTATGTGTC+AGG + chr1_4:34739992-34740011 Msa0160930:intron 45.0%
TTTGAGCAGCGAAAATGCAG+AGG + chr1_4:34740183-34740202 Msa0160930:intron 45.0%
AACAGAAAATCTTCAGGCGG+TGG - chr1_4:34740209-34740228 None:intergenic 45.0%
AAAGAGACCAAACCCCATCT+TGG + chr1_4:34740230-34740249 Msa0160930:CDS 45.0%
! ATTTTTCCCCCTCCAAGATG+GGG - chr1_4:34740245-34740264 None:intergenic 45.0%
! GATTTTTCCCCCTCCAAGAT+GGG - chr1_4:34740246-34740265 None:intergenic 45.0%
! AGATTTTTCCCCCTCCAAGA+TGG - chr1_4:34740247-34740266 None:intergenic 45.0%
CTCAACAAGGTCATATGTGC+TGG - chr1_4:34740285-34740304 None:intergenic 45.0%
GGTATTGCATCTGCTCAACA+AGG - chr1_4:34740298-34740317 None:intergenic 45.0%
TGTGAGGGTTGTTAAGGCTA+AGG + chr1_4:34740322-34740341 Msa0160930:CDS 45.0%
GGCTAAGGATTTGCCTTCAA+AGG + chr1_4:34740337-34740356 Msa0160930:CDS 45.0%
CCTTATGCCGAAGTTAAGCT+CGG + chr1_4:34740380-34740399 Msa0160930:CDS 45.0%
CTTATGCCGAAGTTAAGCTC+GGG + chr1_4:34740381-34740400 Msa0160930:CDS 45.0%
GTTGTGAAGGACGACTTCAT+TGG + chr1_4:34740527-34740546 Msa0160930:CDS 45.0%
TCCTCCAACCTATACCATTG+CGG - chr1_4:34740614-34740633 None:intergenic 45.0%
GGAGGATAGAAAGGGTGATA+AGG + chr1_4:34740628-34740647 Msa0160930:CDS 45.0%
GGTTAAGGGAGAGTTAATGC+TGG + chr1_4:34740649-34740668 Msa0160930:CDS 45.0%
! GAGAGTTAATGCTGGCTGTT+TGG + chr1_4:34740657-34740676 Msa0160930:CDS 45.0%
! GTTAATGCTGGCTGTTTGGA+TGG + chr1_4:34740661-34740680 Msa0160930:CDS 45.0%
AAAATCAGCGGCTCCTCAAA+TGG - chr1_4:34740977-34740996 None:intergenic 45.0%
CTCCTGTCCATAAACTGCAA+AGG - chr1_4:34741058-34741077 None:intergenic 45.0%
ACCACAAACCGGTGAATACT+AGG + chr1_4:34741083-34741102 Msa0160930:CDS 45.0%
!! ACCTAGTATTCACCGGTTTG+TGG - chr1_4:34741087-34741106 None:intergenic 45.0%
ACAAACCGGTGAATACTAGG+TGG + chr1_4:34741086-34741105 Msa0160930:CDS 45.0%
CACATGAGGGTTTGTCTAGA+AGG + chr1_4:34741178-34741197 Msa0160930:CDS 45.0%
ATGAGGGTTTGTCTAGAAGG+TGG + chr1_4:34741181-34741200 Msa0160930:CDS 45.0%
TCCAGATTTCCAGAGCTGTT+TGG - chr1_4:34741260-34741279 None:intergenic 45.0%
!! CAGCTCTGGAAATCTGGAAT+TGG + chr1_4:34741262-34741281 Msa0160930:CDS 45.0%
AGTTGGGATATTGAGTGCTC+AGG + chr1_4:34741294-34741313 Msa0160930:CDS 45.0%
GTTGGGATATTGAGTGCTCA+GGG + chr1_4:34741295-34741314 Msa0160930:CDS 45.0%
!! TTGATAGCTTTATGCCGAGG+TGG + chr1_4:34741413-34741432 Msa0160930:CDS 45.0%
! CTGGAGGTGCAAAAGATTCA+AGG + chr1_4:34741527-34741546 Msa0160930:CDS 45.0%
GTGAAGAACTAGGAGTGGAT+AGG - chr1_4:34741611-34741630 None:intergenic 45.0%
CCCAAGTGGTGTGAAGAAAA+TGG + chr1_4:34741630-34741649 Msa0160930:CDS 45.0%
! CCCATTTTCTTCACACCACT+TGG - chr1_4:34741634-34741653 None:intergenic 45.0%
TAGTGCATCTTTGGCAACAG+AGG - chr1_4:34741724-34741743 None:intergenic 45.0%
TCAACTACCTCCTTTCTCAG+CGG - chr1_4:34741835-34741854 None:intergenic 45.0%
GTGGAGTATGAGAAGAAGCA+AGG + chr1_4:34741882-34741901 Msa0160930:CDS 45.0%
!! ATTGAGTGGGCTAATTGCTG+CGG + chr1_4:34741930-34741949 Msa0160930:CDS 45.0%
GCCAAATTCCAATCAGGAAG+AGG - chr1_4:34742074-34742093 None:intergenic 45.0%
TTCATCCGGATGAGCTGAAT+CGG - chr1_4:34742151-34742170 None:intergenic 45.0%
TTGACACATTCCCGACTTCA+CGG + chr1_4:34742184-34742203 Msa0160930:CDS 45.0%
CTCATCCGAACAATGTCAGA+AGG - chr1_4:34742210-34742229 None:intergenic 45.0%
TTGGCGACTTAGCTACTCAA+GGG + chr1_4:34742274-34742293 Msa0160930:CDS 45.0%
ACTTAGCTACTCAAGGGGAA+AGG + chr1_4:34742280-34742299 Msa0160930:CDS 45.0%
TGTGACTCCATTCCAAGTTG+TGG + chr1_4:34742386-34742405 Msa0160930:CDS 45.0%
! AAGAAATTAAGCGGCACCGA+CGG - chr1_4:34742465-34742484 None:intergenic 45.0%
! TGCCGCTTAATTTCTTCAGG+AGG + chr1_4:34742469-34742488 Msa0160930:CDS 45.0%
AGCATGCAATCAGTTCTTGC+AGG - chr1_4:34742498-34742517 None:intergenic 45.0%
!!! CATATTTTTAAAATTATTAT+CGG + chr1_4:34739971-34739990 Msa0160930:intron 5.0%
CGACATGATACCGAGACATG+TGG + chr1_4:34739914-34739933 Msa0160930:intron 50.0%
AGACCAAACCCCATCTTGGA+GGG + chr1_4:34740234-34740253 Msa0160930:CDS 50.0%
CTTGGAGGGGGAAAAATCTC+TGG + chr1_4:34740248-34740267 Msa0160930:CDS 50.0%
CTGCCGGTAACATCCTTTGA+AGG - chr1_4:34740353-34740372 None:intergenic 50.0%
GGCATAAGGATCACAACTGC+CGG - chr1_4:34740369-34740388 None:intergenic 50.0%
CCGAGCTTAACTTCGGCATA+AGG - chr1_4:34740383-34740402 None:intergenic 50.0%
ACGACTTCATTGGTCGTGTG+TGG + chr1_4:34740537-34740556 Msa0160930:CDS 50.0%
! ATCGGTTCCACTAACAGTCG+CGG - chr1_4:34740732-34740751 None:intergenic 50.0%
GGACTAGAATCTCTCAGAGC+AGG + chr1_4:34740903-34740922 Msa0160930:CDS 50.0%
!! GCAGGAGTATCAATCCGTTG+TGG + chr1_4:34740921-34740940 Msa0160930:CDS 50.0%
TGTTGCTGCGGAACCATTTG+AGG + chr1_4:34740961-34740980 Msa0160930:CDS 50.0%
!! GCCAAACAGCTCTGGAAATC+TGG + chr1_4:34741256-34741275 Msa0160930:CDS 50.0%
! GGCAGAAGTGGATTCGAACA+AGG + chr1_4:34741383-34741402 Msa0160930:CDS 50.0%
TTGCATGGTCCTGACAAAGC+TGG + chr1_4:34741508-34741527 Msa0160930:CDS 50.0%
! TTTGCACCTCCAGCTTTGTC+AGG - chr1_4:34741520-34741539 None:intergenic 50.0%
CTTGGGTGAAGAACTAGGAG+TGG - chr1_4:34741616-34741635 None:intergenic 50.0%
! CTCCTAGTTCTTCACCCAAG+TGG + chr1_4:34741616-34741635 Msa0160930:CDS 50.0%
CACCACTTGGGTGAAGAACT+AGG - chr1_4:34741621-34741640 None:intergenic 50.0%
CAAACTGTCGAGCTGGCTAA+CGG - chr1_4:34741761-34741780 None:intergenic 50.0%
TTAGCCAGCTCGACAGTTTG+AGG + chr1_4:34741761-34741780 Msa0160930:CDS 50.0%
!! TTGAGTGGGCTAATTGCTGC+GGG + chr1_4:34741931-34741950 Msa0160930:CDS 50.0%
GGCTAATTGCTGCGGGAAAA+TGG + chr1_4:34741938-34741957 Msa0160930:CDS 50.0%
!! TCCATGTTAGGAGGGTGTCT+TGG - chr1_4:34742111-34742130 None:intergenic 50.0%
ACCCTCCTAACATGGACACT+CGG + chr1_4:34742115-34742134 Msa0160930:CDS 50.0%
ATGTCAGAAGGCCGTGAAGT+CGG - chr1_4:34742198-34742217 None:intergenic 50.0%
CACGGCCTTCTGACATTGTT+CGG + chr1_4:34742202-34742221 Msa0160930:CDS 50.0%
! GATCGACTTCGAAGCATAGC+TGG + chr1_4:34742234-34742253 Msa0160930:CDS 50.0%
GACTTCGAAGCATAGCTGGT+AGG + chr1_4:34742238-34742257 Msa0160930:CDS 50.0%
AGCTGGTAGGATACAGACTG+TGG + chr1_4:34742251-34742270 Msa0160930:CDS 50.0%
GGTAGGATACAGACTGTGGT+TGG + chr1_4:34742255-34742274 Msa0160930:CDS 50.0%
GTTGGCGACTTAGCTACTCA+AGG + chr1_4:34742273-34742292 Msa0160930:CDS 50.0%
TGGCGACTTAGCTACTCAAG+GGG + chr1_4:34742275-34742294 Msa0160930:CDS 50.0%
! ACAAACAGTGCCGTTGCTCT+TGG - chr1_4:34742333-34742352 None:intergenic 50.0%
AGAAGGGCCACAACTTGGAA+TGG - chr1_4:34742396-34742415 None:intergenic 50.0%
! GGAAGCTTATGACGGAACCT+CGG - chr1_4:34742444-34742463 None:intergenic 50.0%
GTTCCGTCATAAGCTTCCGT+CGG + chr1_4:34742446-34742465 Msa0160930:CDS 50.0%
!! CGGTGCCGCTTAATTTCTTC+AGG + chr1_4:34742466-34742485 Msa0160930:CDS 50.0%
GAGACCAAACCCCATCTTGG+AGG + chr1_4:34740233-34740252 Msa0160930:CDS 55.0%
GACCAAACCCCATCTTGGAG+GGG + chr1_4:34740235-34740254 Msa0160930:CDS 55.0%
ACCAAACCCCATCTTGGAGG+GGG + chr1_4:34740236-34740255 Msa0160930:CDS 55.0%
! TTTCTCGAAGGACCGGCTTC+AGG + chr1_4:34740469-34740488 Msa0160930:CDS 55.0%
CACAGTAACCTCCAACACCG+AGG - chr1_4:34740495-34740514 None:intergenic 55.0%
CTATCTGGGGGAACCCGTTT+CGG - chr1_4:34740581-34740600 None:intergenic 55.0%
GGTTCCCCCAGATAGTCCTT+TGG + chr1_4:34740586-34740605 Msa0160930:CDS 55.0%
ATAGTCCTTTGGCGCCGCAA+TGG + chr1_4:34740597-34740616 Msa0160930:CDS 55.0%
CTATACCATTGCGGCGCCAA+AGG - chr1_4:34740605-34740624 None:intergenic 55.0%
CTTTGGCGCCGCAATGGTAT+AGG + chr1_4:34740603-34740622 Msa0160930:CDS 55.0%
! GCCGCAATGGTATAGGTTGG+AGG + chr1_4:34740610-34740629 Msa0160930:CDS 55.0%
GGCTGTTTGGATGGGTACAC+AGG + chr1_4:34740670-34740689 Msa0160930:CDS 55.0%
GCATCTGAGTGCCAAGCTTC+TGG - chr1_4:34740710-34740729 None:intergenic 55.0%
TCAGATGCCGCGACTGTTAG+TGG + chr1_4:34740722-34740741 Msa0160930:CDS 55.0%
!!! GGAGCCGCTGATTTTGAGTG+TGG + chr1_4:34740982-34741001 Msa0160930:CDS 55.0%
CAGGAGACTCGACCACAAAC+CGG + chr1_4:34741072-34741091 Msa0160930:CDS 55.0%
CAGCAGTGATCTTCGCCCAA+CGG + chr1_4:34741234-34741253 Msa0160930:CDS 55.0%
TCCAGAGCTGTTTGGCCGTT+GGG - chr1_4:34741252-34741271 None:intergenic 55.0%
TTCCAGAGCTGTTTGGCCGT+TGG - chr1_4:34741253-34741272 None:intergenic 55.0%
CATGGTCCTGACAAAGCTGG+AGG + chr1_4:34741511-34741530 Msa0160930:CDS 55.0%
TCGAGCTGGCTAACGGTCAA+CGG - chr1_4:34741754-34741773 None:intergenic 55.0%
GATGCCTCAAACTGTCGAGC+TGG - chr1_4:34741768-34741787 None:intergenic 55.0%
ACTACCTCCTTTCTCAGCGG+TGG - chr1_4:34741832-34741851 None:intergenic 55.0%
GCCAAGACACCCTCCTAACA+TGG + chr1_4:34742107-34742126 Msa0160930:CDS 55.0%
GCCGAGTGTCCATGTTAGGA+GGG - chr1_4:34742119-34742138 None:intergenic 55.0%
AGCCGAGTGTCCATGTTAGG+AGG - chr1_4:34742120-34742139 None:intergenic 55.0%
GAGAGCCGAGTGTCCATGTT+AGG - chr1_4:34742123-34742142 None:intergenic 55.0%
!! TCACGCCGATTCAGCTCATC+CGG + chr1_4:34742143-34742162 Msa0160930:CDS 55.0%
TGTCAGAAGGCCGTGAAGTC+GGG - chr1_4:34742197-34742216 None:intergenic 55.0%
GGCTGCAGTCTTTACTGAGC+TGG + chr1_4:34742301-34742320 Msa0160930:CDS 55.0%
CTGGCGTGATCCAAGAGCAA+CGG + chr1_4:34742320-34742339 Msa0160930:CDS 55.0%
CAAGTTGTGGCCCTTCTGAC+CGG + chr1_4:34742399-34742418 Msa0160930:CDS 55.0%
! GCACCGACGGAAGCTTATGA+CGG - chr1_4:34742452-34742471 None:intergenic 55.0%
TCCCCCTCCAAGATGGGGTT+TGG - chr1_4:34740240-34740259 None:intergenic 60.0%
GGCGCCAAAGGACTATCTGG+GGG - chr1_4:34740593-34740612 None:intergenic 60.0%
CGGCGCCAAAGGACTATCTG+GGG - chr1_4:34740594-34740613 None:intergenic 60.0%
GCGGCGCCAAAGGACTATCT+GGG - chr1_4:34740595-34740614 None:intergenic 60.0%
TGCGGCGCCAAAGGACTATC+TGG - chr1_4:34740596-34740615 None:intergenic 60.0%
GGCGCCGCAATGGTATAGGT+TGG + chr1_4:34740607-34740626 Msa0160930:CDS 60.0%
CGAGCTGGCTAACGGTCAAC+GGG - chr1_4:34741753-34741772 None:intergenic 60.0%
TGAGCCACCGCTGAGAAAGG+AGG + chr1_4:34741825-34741844 Msa0160930:CDS 60.0%
CGGTCAGAAGGGCCACAACT+TGG - chr1_4:34742401-34742420 None:intergenic 60.0%
! GAAGGACCGGCTTCAGGCCT+CGG + chr1_4:34740475-34740494 Msa0160930:CDS 65.0%
CCAACACCGAGGCCTGAAGC+CGG - chr1_4:34740484-34740503 None:intergenic 65.0%
!! GCTTCAGGCCTCGGTGTTGG+AGG + chr1_4:34740484-34740503 Msa0160930:CDS 65.0%
GCCCAACGGCCAAACAGCTC+TGG + chr1_4:34741248-34741267 Msa0160930:CDS 65.0%
CGCTGAGCCACCGCTGAGAA+AGG + chr1_4:34741822-34741841 Msa0160930:CDS 65.0%
! CCGGCTTCAGGCCTCGGTGT+TGG + chr1_4:34740481-34740500 Msa0160930:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 34739065 34742518 34739065 ID=Msa0160930;Name=Msa0160930
chr1_4 mRNA 34739065 34742518 34739065 ID=Msa0160930-mRNA-1;Parent=Msa0160930;Name=Msa0160930-mRNA-1;_AED=0.07;_eAED=0.07;_QI=189|0.5|0.66|1|0|0|3|0|774
chr1_4 exon 34739065 34739180 34739065 ID=Msa0160930-mRNA-1:exon:10605;Parent=Msa0160930-mRNA-1
chr1_4 exon 34739628 34739695 34739628 ID=Msa0160930-mRNA-1:exon:10606;Parent=Msa0160930-mRNA-1
chr1_4 exon 34740192 34742518 34740192 ID=Msa0160930-mRNA-1:exon:10607;Parent=Msa0160930-mRNA-1
chr1_4 five_prime_UTR 34739065 34739180 34739065 ID=Msa0160930-mRNA-1:five_prime_utr;Parent=Msa0160930-mRNA-1
chr1_4 five_prime_UTR 34739628 34739695 34739628 ID=Msa0160930-mRNA-1:five_prime_utr;Parent=Msa0160930-mRNA-1
chr1_4 five_prime_UTR 34740192 34740196 34740192 ID=Msa0160930-mRNA-1:five_prime_utr;Parent=Msa0160930-mRNA-1
chr1_4 CDS 34740197 34742518 34740197 ID=Msa0160930-mRNA-1:cds;Parent=Msa0160930-mRNA-1
Gene Sequence

>Msa0160930

ATGCAGAGGCCACCGCCTGAAGATTTTCTGTTGAAAGAGACCAAACCCCATCTTGGAGGGGGAAAAATCTCTGGTGACAAACTAACCAGCACATATGACCTTGTTGAGCAGATGCAATACCTTTATGTGAGGGTTGTTAAGGCTAAGGATTTGCCTTCAAAGGATGTTACCGGCAGTTGTGATCCTTATGCCGAAGTTAAGCTCGGGAACTATAAAGGAACTACTCGGCATTTCGAGAAGAAAACCAATCCTGAATGGAATCAGGTTTTTGCTTTCTCGAAGGACCGGCTTCAGGCCTCGGTGTTGGAGGTTACTGTGAAAGATAAGGATGTTGTGAAGGACGACTTCATTGGTCGTGTGTGGTTTGATTTGAATGAGGTTCCGAAACGGGTTCCCCCAGATAGTCCTTTGGCGCCGCAATGGTATAGGTTGGAGGATAGAAAGGGTGATAAGGTTAAGGGAGAGTTAATGCTGGCTGTTTGGATGGGTACACAGGCTGATGAAGCATTTCCAGAAGCTTGGCACTCAGATGCCGCGACTGTTAGTGGAACCGATGCTCTTGCAAATATTAGGTCGAAAGTTTATTTATCTCCTAAGCTTTGGTATTTGAGGGTTAATGTGATTGAGGCACAAGACTTACAGCCAACTGATAAGGGTAGATATCCGGAAGTTTTTGTGAAGGCTATTTTAGGGAATCAGGCATTGAGGACTAGAATCTCTCAGAGCAGGAGTATCAATCCGTTGTGGAATGAGGATTTAATGTTTGTTGCTGCGGAACCATTTGAGGAGCCGCTGATTTTGAGTGTGGAAGACAGAGTTGCTCCGAACAAAGAAGAAGTATTGGGGAGATGTGCAATTCCTTTGCAGTTTATGGACAGGAGACTCGACCACAAACCGGTGAATACTAGGTGGTTTAATCTTGAAAAACATATCATTGTAGAAGGGGAAAAGAAGAAAGAAATTAAATTTGCAAGTAGGATTCACATGAGGGTTTGTCTAGAAGGTGGTTATCATGTTTTGGATGAATCAACTCACTACAGCAGTGATCTTCGCCCAACGGCCAAACAGCTCTGGAAATCTGGAATTGGTGTTCTTGAAGTTGGGATATTGAGTGCTCAGGGCTTGATGCCGATGAAAAATAAAGATGGGAGGGGGACAACTGATGCTTATTGTGTAGCAAAATATGGGCAGAAGTGGATTCGAACAAGGACAATCATTGATAGCTTTATGCCGAGGTGGAATGAGCAATATACTTGGGAGGTTTTTGATCCTTGCACTGTGATTACAATTGGTGTATTTGATAATTGTCATTTGCATGGTCCTGACAAAGCTGGAGGTGCAAAAGATTCAAGGATTGGTAAGGTAAGGATTCGACTTTCGACACTCGAGACTGATCGAGTGTACACACATTCCTATCCACTCCTAGTTCTTCACCCAAGTGGTGTGAAGAAAATGGGTGAAATTCAATTGGCTGTGAGGTTCACTTGTTCTTCTTTGCTCAACATGATGCACATGTATTCACTACCTCTGTTGCCAAAGATGCACTATATACACCCGTTGACCGTTAGCCAGCTCGACAGTTTGAGGCATCAAGCTACTCAGATAGTTTCAATGAGGCTGAGTCGCGCTGAGCCACCGCTGAGAAAGGAGGTAGTTGAGTATATGCTTGATGTTGGTTCTCACATGTGGAGTATGAGAAGAAGCAAGGCTAATTTCTTCAGAATCATGGGAGTATTGAGTGGGCTAATTGCTGCGGGAAAATGGTTTGATCAAATTTGCAATTGGAAAAATCCAATTACAACAGTTCTGATTCATATCTTGTTTATAATATTGGTCATGTATCCTGAACTTATCTTACCAACAGTTTTCCTTTACCTCTTCCTGATTGGAATTTGGCACTATAGATGGAGGCCAAGACACCCTCCTAACATGGACACTCGGCTCTCTCACGCCGATTCAGCTCATCCGGATGAACTTGATGAAGAGTTTGACACATTCCCGACTTCACGGCCTTCTGACATTGTTCGGATGAGATATGATCGACTTCGAAGCATAGCTGGTAGGATACAGACTGTGGTTGGCGACTTAGCTACTCAAGGGGAAAGGCTGCAGTCTTTACTGAGCTGGCGTGATCCAAGAGCAACGGCACTGTTTGTGATTTTCTGTCTGATTGCTGCTACTATACTGTATGTGACTCCATTCCAAGTTGTGGCCCTTCTGACCGGAATTTATGTGTTGAGACATCCGAGGTTCCGTCATAAGCTTCCGTCGGTGCCGCTTAATTTCTTCAGGAGGCTGCCTGCAAGAACTGATTGCATGCTT
Protein sequence

>Msa0160930

MQRPPPEDFLLKETKPHLGGGKISGDKLTSTYDLVEQMQYLYVRVVKAKDLPSKDVTGSCDPYAEVKLGNYKGTTRHFEKKTNPEWNQVFAFSKDRLQASVLEVTVKDKDVVKDDFIGRVWFDLNEVPKRVPPDSPLAPQWYRLEDRKGDKVKGELMLAVWMGTQADEAFPEAWHSDAATVSGTDALANIRSKVYLSPKLWYLRVNVIEAQDLQPTDKGRYPEVFVKAILGNQALRTRISQSRSINPLWNEDLMFVAAEPFEEPLILSVEDRVAPNKEEVLGRCAIPLQFMDRRLDHKPVNTRWFNLEKHIIVEGEKKKEIKFASRIHMRVCLEGGYHVLDESTHYSSDLRPTAKQLWKSGIGVLEVGILSAQGLMPMKNKDGRGTTDAYCVAKYGQKWIRTRTIIDSFMPRWNEQYTWEVFDPCTVITIGVFDNCHLHGPDKAGGAKDSRIGKVRIRLSTLETDRVYTHSYPLLVLHPSGVKKMGEIQLAVRFTCSSLLNMMHMYSLPLLPKMHYIHPLTVSQLDSLRHQATQIVSMRLSRAEPPLRKEVVEYMLDVGSHMWSMRRSKANFFRIMGVLSGLIAAGKWFDQICNWKNPITTVLIHILFIILVMYPELILPTVFLYLFLIGIWHYRWRPRHPPNMDTRLSHADSAHPDELDEEFDTFPTSRPSDIVRMRYDRLRSIAGRIQTVVGDLATQGERLQSLLSWRDPRATALFVIFCLIAATILYVTPFQVVALLTGIYVLRHPRFRHKLPSVPLNFFRRLPARTDCML