AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0185050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 226 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTCCTTTGCAGTTTA+TGG 0.091860 1_4:-74955777 Msa0185050:three_prime_UTR
ACCTCTTCCTGATTGGAATT+TGG 0.179060 1_4:-74954755 Msa0185050:three_prime_UTR
TGAATCAGAACTGTTGTAAT+TGG 0.188345 1_4:+74954837 None:intergenic
CTGGGAAAACTTGATGAAAT+TGG 0.190718 1_4:-74957460 Msa0185050:CDS
TCATATCTTGTTCATAATAT+TGG 0.200473 1_4:-74954817 Msa0185050:three_prime_UTR
TGCGGCGCCAAAGGACTATC+TGG 0.234328 1_4:+74956232 None:intergenic
GCTTATTGTGTAGCGAAATA+TGG 0.253558 1_4:-74955464 Msa0185050:three_prime_UTR
GACTAATTGCTGCGGGAAAA+TGG 0.273762 1_4:-74954887 Msa0185050:three_prime_UTR
CCCAAGTGGTGTGAAGAAAA+TGG 0.290205 1_4:-74955195 Msa0185050:three_prime_UTR
GCCAAACAGCTCTGGAAATC+TGG 0.301858 1_4:-74955569 Msa0185050:three_prime_UTR
CACGGCCTTCTGACATTGTT+CGG 0.307253 1_4:-74954623 Msa0185050:three_prime_UTR
CGGTGCCGCTTAATTTCTTC+AGG 0.307382 1_4:-74954359 Msa0185050:three_prime_UTR
AGAAGGTGGTTATCATGTTT+TGG 0.311177 1_4:-74955630 Msa0185050:three_prime_UTR
GCATCCGAGTGCCAAGCTTC+TGG 0.322766 1_4:+74956118 None:intergenic
CACATGAGGGTTTGTCTAGA+AGG 0.326790 1_4:-74955647 Msa0185050:three_prime_UTR
TAGAAAGGGTGATAAGGTTA+AGG 0.328443 1_4:-74956191 Msa0185050:three_prime_UTR
CCCAATACTTCTTCTTTGTT+CGG 0.334931 1_4:+74955806 None:intergenic
CTCCTAAGCTTTGGTATTTG+AGG 0.337491 1_4:-74956039 Msa0185050:three_prime_UTR
TTTCTCCAAGGATCGGCTTC+AGG 0.340930 1_4:-74956356 Msa0185050:three_prime_UTR
TTGGAATTTGGCACTATAGA+TGG 0.341735 1_4:-74954743 Msa0185050:three_prime_UTR
CAAGGATCGGCTTCAGGCCT+CGG 0.347948 1_4:-74956350 Msa0185050:three_prime_UTR
GTGCCTTGAAAGAAAGCTTT+TGG 0.352266 1_4:-74957218 Msa0185050:CDS
AAACCAAAAGCTTTCTTTCA+AGG 0.356098 1_4:+74957215 None:intergenic
TCCAGAGCTGTTTGGCCGTT+GGG 0.357398 1_4:+74955576 None:intergenic
ATTGTCCATCATTATGTCAT+AGG 0.358719 1_4:-74954198 Msa0185050:three_prime_UTR
CCGAACAAAGAAGAAGTATT+GGG 0.371136 1_4:-74955806 Msa0185050:three_prime_UTR
TTCCTTTACCTCTTCCTGAT+TGG 0.374360 1_4:-74954762 Msa0185050:three_prime_UTR
CCGATGCTCTTGCAAATATT+AGG 0.375111 1_4:-74956078 Msa0185050:three_prime_UTR
GTTAAGCTCGGGAACTATAA+AGG 0.383225 1_4:-74956433 Msa0185050:three_prime_UTR
TTTATTTATCTCCTAAGCTT+TGG 0.384714 1_4:-74956048 Msa0185050:three_prime_UTR
TTTGCACCTCCAGCTTTGTC+AGG 0.385021 1_4:+74955308 None:intergenic
GGCTAATTTCTTCAGAATCA+TGG 0.388398 1_4:-74954922 Msa0185050:three_prime_UTR
TCCTAAGCTTTGGTATTTGA+GGG 0.393359 1_4:-74956038 Msa0185050:three_prime_UTR
CCTTTACGTGAGGGTTGTTA+AGG 0.393627 1_4:-74956509 Msa0185050:three_prime_UTR
CAGCTCTGGAAATCTGGAAT+TGG 0.397726 1_4:-74955563 Msa0185050:three_prime_UTR
GAGAGCCGAGTGTCCATGTT+AGG 0.399650 1_4:+74954705 None:intergenic
GGTTCGATCAAATTTGCAAC+TGG 0.404361 1_4:-74954866 Msa0185050:three_prime_UTR
CTCTGCACTGTCCTTTGTTG+AGG 0.408531 1_4:+74954294 None:intergenic
ACAAACAGTGCTGTTGCTCT+TGG 0.411446 1_4:+74954495 None:intergenic
CGTTGCTGCGGAACCATTTG+AGG 0.412932 1_4:-74955864 Msa0185050:three_prime_UTR
CGAGGCCTGAAGCCGATCCT+TGG 0.414112 1_4:+74956351 None:intergenic
GCCCAACGGCCAAACAGCTC+TGG 0.415222 1_4:-74955577 Msa0185050:three_prime_UTR
GGAATTGGTGTTCTTGAAGT+TGG 0.415516 1_4:-74955548 Msa0185050:three_prime_UTR
TTTGAATGAGGTTCCGAAAC+GGG 0.416032 1_4:-74956260 Msa0185050:three_prime_UTR
GAGAGTTAATGCTTGCTGTT+TGG 0.419766 1_4:-74956168 Msa0185050:three_prime_UTR
TCGGATGCCGCGACTGTTAG+TGG 0.420415 1_4:-74956103 Msa0185050:three_prime_UTR
TTGCCTTCAAAGGATGTTAC+CGG 0.421207 1_4:-74956478 Msa0185050:three_prime_UTR
GGGTGTATATAGTGCATCTT+TGG 0.421466 1_4:+74955095 None:intergenic
TCTTTCAACAGAAAATCTTC+AGG 0.424219 1_4:+74956613 None:intergenic
AACTCTGAAATCAGAGATCT+AGG 0.426167 1_4:-74957190 Msa0185050:intron
AACCAATCCTGAATGGAATC+AGG 0.430195 1_4:-74956386 Msa0185050:three_prime_UTR
AGACTTACAGCCAACTGATA+AGG 0.432910 1_4:-74955996 Msa0185050:three_prime_UTR
TGTCAGAAGGCCGTGAAGTC+GGG 0.434203 1_4:+74954631 None:intergenic
GGCTGCAGTCTTTGCTGAGC+TGG 0.435790 1_4:-74954524 Msa0185050:three_prime_UTR
AAAATCAGCGGCTCCTCAAA+TGG 0.439031 1_4:+74955851 None:intergenic
TTCCAGAGCTGTTTGGCCGT+TGG 0.439868 1_4:+74955575 None:intergenic
GTATAGATTGGAGGATAGAA+AGG 0.441691 1_4:-74956206 Msa0185050:three_prime_UTR
TCAACAAAATCTACTGTCTT+CGG 0.443642 1_4:+74954230 None:intergenic
TCGGCTTCAGGCCTCGGTGT+TGG 0.445911 1_4:-74956344 Msa0185050:three_prime_UTR
CGCTGAGCCACCGCTGAGAA+AGG 0.446361 1_4:-74955003 Msa0185050:three_prime_UTR
TCCAGATTTCCAGAGCTGTT+TGG 0.447417 1_4:+74955568 None:intergenic
AAGAAATTAAATTTGCAAGT+AGG 0.449686 1_4:-74955673 Msa0185050:three_prime_UTR
AGTTGGGATATTGAGTGCTC+AGG 0.452833 1_4:-74955531 Msa0185050:three_prime_UTR
CTTATTGTGTAGCGAAATAT+GGG 0.456005 1_4:-74955463 Msa0185050:three_prime_UTR
GACTTACAGCCAACTGATAA+GGG 0.456275 1_4:-74955995 Msa0185050:three_prime_UTR
GAATTGGTGTTCTTGAAGTT+GGG 0.456895 1_4:-74955547 Msa0185050:three_prime_UTR
TTCGTCCGGATGAGCTGAAT+CGG 0.463142 1_4:+74954677 None:intergenic
AGAAAGGGTGATAAGGTTAA+GGG 0.464699 1_4:-74956190 Msa0185050:three_prime_UTR
GGCGCCGCAATGGTATAGAT+TGG 0.464850 1_4:-74956218 Msa0185050:three_prime_UTR
TGCTGTTTGGATGGGTACAC+AGG 0.468622 1_4:-74956155 Msa0185050:three_prime_UTR
TCGACACATTCCCGACTTCA+CGG 0.468689 1_4:-74954641 Msa0185050:three_prime_UTR
TATAGTGCCAAATTCCAATC+AGG 0.471795 1_4:+74954748 None:intergenic
CCTTGCACTGTGATTACAAT+TGG 0.473689 1_4:-74955359 Msa0185050:three_prime_UTR
AACTGATAAGGGTAGATATC+CGG 0.474666 1_4:-74955984 Msa0185050:three_prime_UTR
CGAGCTGGCTAACGGTCAAC+GGG 0.475727 1_4:+74955075 None:intergenic
AACTCTTCATCAAGTTCGTC+CGG 0.476174 1_4:+74954663 None:intergenic
ATTCAAGGATTGGTAAGGTA+AGG 0.476487 1_4:-74955283 Msa0185050:three_prime_UTR
GAAAATGGGTGAAATTCAAT+TGG 0.477497 1_4:-74955180 Msa0185050:three_prime_UTR
ACTGTTGGTAAGATAAGTTC+AGG 0.477704 1_4:+74954786 None:intergenic
GCTTCAGGCCTCGGTGTTGG+AGG 0.478715 1_4:-74956341 Msa0185050:three_prime_UTR
GGAGGATAGAAAGGGTGATA+AGG 0.479059 1_4:-74956197 Msa0185050:three_prime_UTR
GCTAATTTCTTCAGAATCAT+GGG 0.479725 1_4:-74954921 Msa0185050:three_prime_UTR
ACCTAGTATTCACTGGTTTG+TGG 0.480851 1_4:+74955741 None:intergenic
AACCTGATTCCATTCAGGAT+TGG 0.483996 1_4:+74956384 None:intergenic
TTCCAATCAGGAAGAGGTAA+AGG 0.484097 1_4:+74954760 None:intergenic
GGCTAAGGACTTGCCTTCAA+AGG 0.484522 1_4:-74956488 Msa0185050:three_prime_UTR
TTGTCCATCATTATGTCATA+GGG 0.484983 1_4:-74954197 Msa0185050:three_prime_UTR
CGTGAGGGTTGTTAAGGCTA+AGG 0.486394 1_4:-74956503 Msa0185050:three_prime_UTR
CTGGAGGTGCAAAAGATTCA+AGG 0.487820 1_4:-74955298 Msa0185050:three_prime_UTR
ATGAAGCATTTCCAGAAGCT+TGG 0.489119 1_4:-74956129 Msa0185050:three_prime_UTR
CGGTCAGAAGGGCCACAACT+TGG 0.491080 1_4:+74954427 None:intergenic
GCGGCGCCAAAGGACTATCT+GGG 0.491089 1_4:+74956233 None:intergenic
GTTGTGAAGGACGACTTCAT+TGG 0.491403 1_4:-74956298 Msa0185050:three_prime_UTR
ACCACAAACCAGTGAATACT+AGG 0.491540 1_4:-74955742 Msa0185050:three_prime_UTR
GTTGAGTATATGCTTGATGT+TGG 0.492238 1_4:-74954975 Msa0185050:three_prime_UTR
ACCCTCAAATACCAAAGCTT+AGG 0.493243 1_4:+74956037 None:intergenic
AATAAAATGTAACATTTCAA+AGG 0.496041 1_4:+74957369 None:intergenic
AAAGAGACCAAACCCCATCT+TGG 0.497107 1_4:-74956595 Msa0185050:three_prime_UTR
ATGTCTCAACACATAAATTC+CGG 0.498537 1_4:+74954407 None:intergenic
CTCAACAAGGTCATATGTGC+TGG 0.498939 1_4:+74956543 None:intergenic
TCCATGTTAGGAGGGTGTCT+TGG 0.501157 1_4:+74954717 None:intergenic
CAAGTTGTGGCCCTTCTGAC+CGG 0.503387 1_4:-74954426 Msa0185050:three_prime_UTR
TCCGAACAAAGAAGAAGTAT+TGG 0.503767 1_4:-74955807 Msa0185050:three_prime_UTR
GTTAATGCTTGCTGTTTGGA+TGG 0.505912 1_4:-74956164 Msa0185050:three_prime_UTR
GGACTAGAATCTCTCTTAGC+AGG 0.511755 1_4:-74955922 Msa0185050:three_prime_UTR
TTAGCCAGCTCGACAGTTTG+AGG 0.514684 1_4:-74955064 Msa0185050:three_prime_UTR
ATGTCAGAAGGCCGTGAAGT+CGG 0.514758 1_4:+74954630 None:intergenic
AACAGAAAATCTTCAGGCGG+TGG 0.514836 1_4:+74956619 None:intergenic
AGAAGAAAACCAATCCTGAA+TGG 0.515488 1_4:-74956393 Msa0185050:three_prime_UTR
GATCGACTTAGAAGCATAGC+TGG 0.518904 1_4:-74954591 Msa0185050:three_prime_UTR
CTTCCGGTAACATCCTTTGA+AGG 0.519491 1_4:+74956475 None:intergenic
GGTGCAAAAGATTCAAGGAT+TGG 0.520817 1_4:-74955293 Msa0185050:three_prime_UTR
TCGAGCTGGCTAACGGTCAA+CGG 0.520943 1_4:+74955074 None:intergenic
TCACGCCGATTCAGCTCATC+CGG 0.522054 1_4:-74954682 Msa0185050:three_prime_UTR
GTTGGGATATTGAGTGCTCA+GGG 0.525396 1_4:-74955530 Msa0185050:three_prime_UTR
CCTTAACAACCCTCACGTAA+AGG 0.525933 1_4:+74956509 None:intergenic
CACCACTTGGGTGAAGAACT+AGG 0.526449 1_4:+74955207 None:intergenic
TTAAACCACCTAGTATTCAC+TGG 0.527121 1_4:+74955734 None:intergenic
AGCCGAGTGTCCATGTTAGG+AGG 0.527424 1_4:+74954708 None:intergenic
TTGCATGGTCCTGACAAAGC+TGG 0.527750 1_4:-74955317 Msa0185050:three_prime_UTR
GCCAAATTCCAATCAGGAAG+AGG 0.528138 1_4:+74954754 None:intergenic
TTTGAGGGTAAATGTGATTG+AGG 0.529044 1_4:-74956023 Msa0185050:three_prime_UTR
AAAACCCTATGACATAATGA+TGG 0.529232 1_4:+74954193 None:intergenic
ACACATAAATTCCGGTCAGA+AGG 0.532406 1_4:+74954415 None:intergenic
CTATACCATTGCGGCGCCAA+AGG 0.532688 1_4:+74956223 None:intergenic
AGCGTACGGATCACAACTTC+CGG 0.532790 1_4:+74956459 None:intergenic
AGCATGCAATCAGTTCTTGC+AGG 0.532832 1_4:+74954330 None:intergenic
TTCCTTTGCAGTTTATGGAT+AGG 0.533408 1_4:-74955772 Msa0185050:three_prime_UTR
CCAAGTGGTGTGAAGAAAAT+GGG 0.533679 1_4:-74955194 Msa0185050:three_prime_UTR
ATTTGAATGAGGTTCCGAAA+CGG 0.535448 1_4:-74956261 Msa0185050:three_prime_UTR
TGCAAGTAGGATTCACATGA+GGG 0.537596 1_4:-74955660 Msa0185050:three_prime_UTR
TTCTTCTCATACTCCACATG+TGG 0.540825 1_4:+74954949 None:intergenic
AAGAAATTAAGCGGCACCGA+CGG 0.541607 1_4:+74954363 None:intergenic
CACATAAATTCCGGTCAGAA+GGG 0.542177 1_4:+74954416 None:intergenic
AGAAGGGCCACAACTTGGAA+TGG 0.547838 1_4:+74954432 None:intergenic
GTGAAGAACTAGGAGTGGAT+AGG 0.551635 1_4:+74955217 None:intergenic
GCCAAGACACCCTCCTAACA+TGG 0.551812 1_4:-74954718 Msa0185050:three_prime_UTR
CCGTACGCTGAAGTTAAGCT+CGG 0.557320 1_4:-74956445 Msa0185050:three_prime_UTR
TGTGTGGTTTGATTTGAATG+AGG 0.557487 1_4:-74956272 Msa0185050:three_prime_UTR
TGCCGCTTAATTTCTTCAGG+AGG 0.557905 1_4:-74954356 Msa0185050:three_prime_UTR
GGATTTGATGTTCGTTGCTG+CGG 0.557925 1_4:-74955876 Msa0185050:three_prime_UTR
TAGCGAAATATGGGCAGAAG+TGG 0.558621 1_4:-74955454 Msa0185050:three_prime_UTR
AAAAGATTCAAGGATTGGTA+AGG 0.560457 1_4:-74955288 Msa0185050:three_prime_UTR
GAAGTTTGAATCCTCAACAA+AGG 0.560974 1_4:-74954305 Msa0185050:three_prime_UTR
CCTAATATTTGCAAGAGCAT+CGG 0.563372 1_4:+74956078 None:intergenic
AGACCAAACCCCATCTTGGA+GGG 0.563491 1_4:-74956591 Msa0185050:three_prime_UTR
GAGACCAAACCCCATCTTGG+AGG 0.564193 1_4:-74956592 Msa0185050:three_prime_UTR
ACGACTTCATTGGTCGTGTG+TGG 0.564266 1_4:-74956288 Msa0185050:three_prime_UTR
TTTGATAATTGTCATTTGCA+TGG 0.564967 1_4:-74955332 Msa0185050:three_prime_UTR
ATAGTCCTTTGGCGCCGCAA+TGG 0.565983 1_4:-74956228 Msa0185050:three_prime_UTR
CTCCTATCCATAAACTGCAA+AGG 0.566217 1_4:+74955770 None:intergenic
GATGCCTCAAACTGTCGAGC+TGG 0.566280 1_4:+74955060 None:intergenic
ATTTCCAGAAGCTTGGCACT+CGG 0.566792 1_4:-74956122 Msa0185050:three_prime_UTR
AAAACATATCATTGTAGAAG+GGG 0.567643 1_4:-74955705 Msa0185050:three_prime_UTR
CCGAGCTTAACTTCAGCGTA+CGG 0.569596 1_4:+74956445 None:intergenic
GCCGCAATGGTATAGATTGG+AGG 0.570899 1_4:-74956215 Msa0185050:three_prime_UTR
GGTGGAATGAGCAATATACT+TGG 0.572552 1_4:-74955394 Msa0185050:three_prime_UTR
GGATATCTACCCTTATCAGT+TGG 0.576446 1_4:+74955986 None:intergenic
TATAGATTGGAGGATAGAAA+GGG 0.576864 1_4:-74956205 Msa0185050:three_prime_UTR
TTGAGTGGACTAATTGCTGC+GGG 0.577897 1_4:-74954894 Msa0185050:three_prime_UTR
CTCCTAGTTCTTCACCCAAG+TGG 0.579462 1_4:-74955209 Msa0185050:three_prime_UTR
CAAACTGTCGAGCTGGCTAA+CGG 0.580833 1_4:+74955067 None:intergenic
TGTGACTCCATTCCAAGTTG+TGG 0.583105 1_4:-74954439 Msa0185050:three_prime_UTR
ACTTAGCTACTCAAGGAGAA+AGG 0.584009 1_4:-74954545 Msa0185050:three_prime_UTR
TTAATGCTTGCTGTTTGGAT+GGG 0.584454 1_4:-74956163 Msa0185050:three_prime_UTR
ATCAAATCCTCGTTCCACAA+TGG 0.587304 1_4:+74955890 None:intergenic
ACCAAACCCCATCTTGGAGG+GGG 0.588344 1_4:-74956589 Msa0185050:three_prime_UTR
TTGCAAGTAGGATTCACATG+AGG 0.591241 1_4:-74955661 Msa0185050:three_prime_UTR
GGCAGAAGTGGATTCGAACA+AGG 0.592797 1_4:-74955442 Msa0185050:three_prime_UTR
TTGATGTTGGTTCCCACATG+TGG 0.601847 1_4:-74954962 Msa0185050:three_prime_UTR
AAGAGGTAAAGGAAAACTGT+TGG 0.606111 1_4:+74954771 None:intergenic
CCAATTGTAATCACAGTGCA+AGG 0.607117 1_4:+74955359 None:intergenic
ACTACCTCCTTTCTCAGCGG+TGG 0.608379 1_4:+74954996 None:intergenic
ACCCTCCTAACATGGACACT+CGG 0.608381 1_4:-74954710 Msa0185050:three_prime_UTR
GGTAGGATACAGACTGTGGT+TGG 0.612469 1_4:-74954570 Msa0185050:three_prime_UTR
CGTACGCTGAAGTTAAGCTC+GGG 0.613305 1_4:-74956444 Msa0185050:three_prime_UTR
GACCAAACCCCATCTTGGAG+GGG 0.614269 1_4:-74956590 Msa0185050:three_prime_UTR
CGGCGCCAAAGGACTATCTG+GGG 0.614676 1_4:+74956234 None:intergenic
GGAAGCTTATGACGGAACCT+CGG 0.615117 1_4:+74954384 None:intergenic
CTCATCCGAACAATGTCAGA+AGG 0.615248 1_4:+74954618 None:intergenic
GGAGGTTACTGTGAAAGATA+AGG 0.615500 1_4:-74956323 Msa0185050:three_prime_UTR
TTCAACAGAAAATCTTCAGG+CGG 0.617196 1_4:+74956616 None:intergenic
AGAATCATGGGAGTATTGAG+TGG 0.619315 1_4:-74954909 Msa0185050:three_prime_UTR
CAGCAGTGATCTTCGCCCAA+CGG 0.622948 1_4:-74955591 Msa0185050:three_prime_UTR
GCCGAGTGTCCATGTTAGGA+GGG 0.626241 1_4:+74954709 None:intergenic
GAATTTGGCACTATAGATGG+AGG 0.626699 1_4:-74954740 Msa0185050:three_prime_UTR
AGATGCAATACCTTTACGTG+AGG 0.626728 1_4:-74956519 Msa0185050:three_prime_UTR
ATGAGGGTTTGTCTAGAAGG+TGG 0.627125 1_4:-74955644 Msa0185050:three_prime_UTR
GTGGAATGAGCAATATACTT+GGG 0.627653 1_4:-74955393 Msa0185050:three_prime_UTR
CTTGGGTGAAGAACTAGGAG+TGG 0.630605 1_4:+74955212 None:intergenic
TGAGCCACCGCTGAGAAAGG+AGG 0.630758 1_4:-74955000 Msa0185050:three_prime_UTR
GACTTAGAAGCATAGCTGGT+AGG 0.632636 1_4:-74954587 Msa0185050:three_prime_UTR
GGTATTGCATCTGCTCAACA+AGG 0.633340 1_4:+74956530 None:intergenic
TAGTGCATCTTTGGCAACAG+AGG 0.634644 1_4:+74955104 None:intergenic
GATGCAATACCTTTACGTGA+GGG 0.638362 1_4:-74956518 Msa0185050:three_prime_UTR
GCACCGACGGAAGCTTATGA+CGG 0.638901 1_4:+74954376 None:intergenic
TTGATAGCTTTATGCCGAGG+TGG 0.644610 1_4:-74955412 Msa0185050:three_prime_UTR
TCCTCCAATCTATACCATTG+CGG 0.645041 1_4:+74956214 None:intergenic
GAATGAGCAATATACTTGGG+AGG 0.645094 1_4:-74955390 Msa0185050:three_prime_UTR
GAAAGATAAGGATGTTGTGA+AGG 0.645624 1_4:-74956311 Msa0185050:three_prime_UTR
GTTGGCGACTTAGCTACTCA+AGG 0.648005 1_4:-74954552 Msa0185050:three_prime_UTR
GCAGGAGTATCAATCCATTG+TGG 0.649222 1_4:-74955904 Msa0185050:three_prime_UTR
GTGGAGTATGAGAAGAAGCA+AGG 0.650662 1_4:-74954943 Msa0185050:three_prime_UTR
ATCGGTTCCACTAACAGTCG+CGG 0.651678 1_4:+74956096 None:intergenic
AGCCTCCTGAAGAAATTAAG+CGG 0.651985 1_4:+74954354 None:intergenic
TTTGAGCAACGAAAATGCAG+AGG 0.652074 1_4:-74956642 Msa0185050:three_prime_UTR
GTTCCGTCATAAGCTTCCGT+CGG 0.653799 1_4:-74954379 Msa0185050:three_prime_UTR
GTGAAATTCAATTGGCTGTG+AGG 0.654400 1_4:-74955172 Msa0185050:three_prime_UTR
GGAACTATAAAGGAACTACT+CGG 0.658774 1_4:-74956423 Msa0185050:three_prime_UTR
ATTGAGTGGACTAATTGCTG+CGG 0.661101 1_4:-74954895 Msa0185050:three_prime_UTR
CTACTCAGATAGTTTCAATG+AGG 0.663957 1_4:-74955034 Msa0185050:three_prime_UTR
TCATTGATAGCTTTATGCCG+AGG 0.666881 1_4:-74955415 Msa0185050:three_prime_UTR
TATCAATCCATTGTGGAACG+AGG 0.672925 1_4:-74955897 Msa0185050:three_prime_UTR
GGCGCCAAAGGACTATCTGG+GGG 0.676642 1_4:+74956235 None:intergenic
CAACAAAGGACAGTGCAGAG+TGG 0.681133 1_4:-74954291 Msa0185050:three_prime_UTR
CGAACAAAGAAGAAGTATTG+GGG 0.685152 1_4:-74955805 Msa0185050:three_prime_UTR
TCTTCCACACTCAAAATCAG+CGG 0.687134 1_4:+74955839 None:intergenic
TCTTCTCATACTCCACATGT+GGG 0.689277 1_4:+74954950 None:intergenic
TTTATGTGTTGAGACATCCG+AGG 0.696759 1_4:-74954401 Msa0185050:three_prime_UTR
AGCTGGTAGGATACAGACTG+TGG 0.703110 1_4:-74954574 Msa0185050:three_prime_UTR
ACAAAGAAGAAGTATTGGGG+AGG 0.704686 1_4:-74955802 Msa0185050:three_prime_UTR
CATGGTCCTGACAAAGCTGG+AGG 0.704809 1_4:-74955314 Msa0185050:three_prime_UTR
GTATATTGCTCATTCCACCT+CGG 0.716433 1_4:+74955398 None:intergenic
TCAACTACCTCCTTTCTCAG+CGG 0.739237 1_4:+74954993 None:intergenic
CACAGTAACCTCCAACACCG+AGG 0.741552 1_4:+74956333 None:intergenic
ACAAACCAGTGAATACTAGG+TGG 0.765072 1_4:-74955739 Msa0185050:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGTTTTCAAGAAAAATT+TGG + chr1_4:74954252-74954271 None:intergenic 15CTACTAAACTAGCAAGTTAG%
!!! TAACATAATTCTTTGAGAAT+TGG + chr1_4:74954152-74954171 None:intergenic 20.0%
!!! TAGTATATCTGTAATTTTGT+AGG - chr1_4:74958499-74958518 Msa0185050:intron 20.0%
!!! AGTATATCTGTAATTTTGTA+GGG - chr1_4:74958500-74958519 Msa0185050:intron 20.0%
!!! ATTTAGTAGTTTTTCTCAAT+GGG - chr1_4:74958570-74958589 Msa0185050:intron 20.0%
!! GATTTAGTAGTTTTTCTCAA+TGG - chr1_4:74958569-74958588 Msa0185050:intron 25.0%
!! TTTAGTAGTTTTTCTCAATG+GGG - chr1_4:74958571-74958590 Msa0185050:intron 25.0%
AAAACCCTATGACATAATGA+TGG + chr1_4:74958457-74958476 None:intergenic 30.0%
!! TGTCATAGGGTTTTATGTTT+TGG - chr1_4:74958463-74958482 Msa0185050:intron 30.0%
! GGGAATTGGTTGAATTTTTA+TGG - chr1_4:74958591-74958610 Msa0185050:intron 30.0%
! AGTTTTTCTCAATGGGGAAT+TGG - chr1_4:74958577-74958596 Msa0185050:intron 35.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 74954051 74958618 74954051 ID=Msa0185050;Name=Msa0185050
chr1_4 mRNA 74954051 74958618 74954051 ID=Msa0185050-mRNA-1;Parent=Msa0185050;Name=Msa0185050-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0.33|0.5|0.5|1|1|4|2669|64
chr1_4 exon 74954051 74956727 74954051 ID=Msa0185050-mRNA-1:exon:25530;Parent=Msa0185050-mRNA-1
chr1_4 exon 74957191 74957258 74957191 ID=Msa0185050-mRNA-1:exon:25529;Parent=Msa0185050-mRNA-1
chr1_4 exon 74957382 74957491 74957382 ID=Msa0185050-mRNA-1:exon:25528;Parent=Msa0185050-mRNA-1
chr1_4 exon 74958610 74958618 74958610 ID=Msa0185050-mRNA-1:exon:25527;Parent=Msa0185050-mRNA-1
chr1_4 CDS 74958610 74958618 74958610 ID=Msa0185050-mRNA-1:cds;Parent=Msa0185050-mRNA-1
chr1_4 CDS 74957382 74957491 74957382 ID=Msa0185050-mRNA-1:cds;Parent=Msa0185050-mRNA-1
chr1_4 CDS 74957191 74957258 74957191 ID=Msa0185050-mRNA-1:cds;Parent=Msa0185050-mRNA-1
chr1_4 CDS 74956720 74956727 74956720 ID=Msa0185050-mRNA-1:cds;Parent=Msa0185050-mRNA-1
chr1_4 three_prime_UTR 74954051 74956719 74954051 ID=Msa0185050-mRNA-1:three_prime_utr;Parent=Msa0185050-mRNA-1
Gene Sequence

>Msa0185050

ATGCAAAAGCAAATATTTCTGGGAAAACTTGATGAAATTGGAAGATCACTAATCAAAGTGTTCATATTTGTTTTCTCATTGCGTAATCTCAGAATGCTGAAAGTTTCAACCTTTGAAATGTTGAACGATCAGATCTTGTGCCTTGAAAGAAAGCTTTTGGTTTGGAACTCTGAAATCAGAGATCTAGGCTTCTGA
Protein sequence

>Msa0185050

MQKQIFLGKLDEIGRSLIKVFIFVFSLRNLRMLKVSTFEMLNDQILCLERKLLVWNSEIRDLGF*