AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0185030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0185030 MtrunA17_Chr1g0174761 99.612 773 3 0 6 778 2 774 0.0 1599
Msa0185030 MtrunA17_Chr7g0258331 93.161 775 50 3 6 778 2 775 0.0 1513
Msa0185030 MtrunA17_Chr4g0033151 82.429 774 130 4 6 778 2 770 0.0 1328
Msa0185030 MtrunA17_Chr3g0087501 68.608 790 225 8 11 778 34 822 0.0 1156
Msa0185030 MtrunA17_Chr4g0010751 71.561 756 209 5 28 778 440 1194 0.0 1153
Msa0185030 MtrunA17_Chr5g0397601 68.726 777 233 4 10 778 233 1007 0.0 1148
Msa0185030 MtrunA17_Chr1g0174581 67.128 794 242 8 3 778 5 797 0.0 1134
Msa0185030 MtrunA17_Chr4g0014711 65.478 785 258 9 1 778 266 1044 0.0 1063
Msa0185030 MtrunA17_Chr8g0349231 60.256 780 292 7 10 778 231 1003 0.0 999
Msa0185030 MtrunA17_Chr4g0028371 58.397 786 304 11 5 778 9 783 0.0 898
Msa0185030 MtrunA17_Chr4g0035961 55.725 786 274 12 5 778 9 732 0.0 862
Msa0185030 MtrunA17_Chr7g0247271 59.137 695 262 8 97 778 281 966 0.0 853
Msa0185030 MtrunA17_Chr4g0060191 53.351 776 318 14 22 778 288 1038 0.0 808
Msa0185030 MtrunA17_Chr6g0460791 52.294 763 317 13 36 778 274 1009 0.0 783
Msa0185030 MtrunA17_Chr6g0461151 48.231 763 357 13 34 778 284 1026 0.0 736
Msa0185030 MtrunA17_Chr1g0151051 48.590 780 369 13 16 778 34 798 0.0 697
Msa0185030 MtrunA17_Chr5g0447321 41.026 780 424 12 12 778 7 763 0.0 593
Msa0185030 MtrunA17_Chr4g0010641 67.692 130 40 1 648 777 1 128 3.97e-54 182
Msa0185030 MtrunA17_Chr4g0010701 50.962 104 29 1 651 754 16 97 2.49e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0185030 AT3G57880.4 91.332 773 66 1 6 778 2 773 0.0 1487
Msa0185030 AT3G57880.2 91.332 773 66 1 6 778 2 773 0.0 1487
Msa0185030 AT3G57880.3 91.332 773 66 1 6 778 2 773 0.0 1487
Msa0185030 AT3G57880.1 91.332 773 66 1 6 778 2 773 0.0 1487
Msa0185030 AT1G51570.1 88.387 775 88 2 6 778 2 776 0.0 1440
Msa0185030 AT5G12970.1 81.877 767 133 3 12 778 9 769 0.0 1310
Msa0185030 AT5G06850.1 68.662 785 228 8 11 778 11 794 0.0 1156
Msa0185030 AT4G11610.1 67.224 778 242 5 10 778 238 1011 0.0 1128
Msa0185030 AT4G11610.2 67.224 778 242 5 10 778 234 1007 0.0 1127
Msa0185030 AT4G11610.3 67.224 778 242 5 10 778 101 874 0.0 1127
Msa0185030 AT5G48060.1 65.897 780 252 8 11 778 259 1036 0.0 1102
Msa0185030 AT1G22610.1 66.367 779 245 8 12 778 256 1029 0.0 1075
Msa0185030 AT4G00700.1 59.079 782 305 6 10 778 227 1006 0.0 966
Msa0185030 AT4G00700.2 59.079 782 305 6 10 778 227 1006 0.0 966
Msa0185030 AT3G61300.1 58.869 778 291 13 8 778 217 972 0.0 945
Msa0185030 AT1G04150.1 58.354 790 288 14 12 778 241 1012 0.0 926
Msa0185030 AT4G20080.1 58.161 772 305 11 13 778 15 774 0.0 921
Msa0185030 AT1G74720.1 52.244 802 345 13 2 778 293 1081 0.0 824
Msa0185030 AT3G03680.1 52.734 768 314 15 36 778 274 1017 0.0 784
Msa0185030 AT5G17980.1 48.810 756 356 12 34 778 314 1049 0.0 749
Msa0185030 AT5G03435.1 40.927 777 402 25 11 772 6 740 3.11e-168 504
Msa0185030 AT3G61720.1 38.985 808 435 21 11 778 6 795 2.43e-167 503
Msa0185030 AT5G03435.2 43.154 577 293 17 11 578 6 556 1.54e-140 426
Msa0185030 AT5G44760.1 50.750 400 150 9 12 404 5 364 1.22e-122 377
Msa0185030 AT5G44760.1 36.496 137 38 1 544 680 364 451 7.24e-20 94.0
Msa0185030 AT1G03370.2 28.980 245 155 11 45 285 3 232 1.46e-12 72.0
Msa0185030 AT1G03370.1 28.980 245 155 11 45 285 3 232 1.67e-12 72.0

Find 213 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTCCTTTGCAGTTTA+TGG 0.091860 1_4:+74871912 Msa0185030:CDS
ACCTCTTCCTGATTGGAATT+TGG 0.179060 1_4:+74872934 Msa0185030:CDS
TGAATCAGAACTGTTGTAAT+TGG 0.188345 1_4:-74872852 None:intergenic
TCATATCTTGTTCATAATAT+TGG 0.200473 1_4:+74872872 Msa0185030:CDS
TGCGGCGCCAAAGGACTATC+TGG 0.234328 1_4:-74871457 None:intergenic
GCTTATTGTGTAGCGAAATA+TGG 0.253558 1_4:+74872225 Msa0185030:CDS
GACTAATTGCTGCGGGAAAA+TGG 0.273762 1_4:+74872802 Msa0185030:CDS
CCCAAGTGGTGTGAAGAAAA+TGG 0.290205 1_4:+74872494 Msa0185030:CDS
GCCAAACAGCTCTGGAAATC+TGG 0.301858 1_4:+74872120 Msa0185030:CDS
CACGGCCTTCTGACATTGTT+CGG 0.307253 1_4:+74873066 Msa0185030:CDS
CGGTGCCGCTTAATTTCTTC+AGG 0.307382 1_4:+74873330 Msa0185030:CDS
AGAAGGTGGTTATCATGTTT+TGG 0.311177 1_4:+74872059 Msa0185030:CDS
GCATCCGAGTGCCAAGCTTC+TGG 0.322766 1_4:-74871571 None:intergenic
CACATGAGGGTTTGTCTAGA+AGG 0.326790 1_4:+74872042 Msa0185030:CDS
TAGAAAGGGTGATAAGGTTA+AGG 0.328443 1_4:+74871498 Msa0185030:CDS
CCCAATACTTCTTCTTTGTT+CGG 0.334931 1_4:-74871883 None:intergenic
CTCCTAAGCTTTGGTATTTG+AGG 0.337491 1_4:+74871650 Msa0185030:CDS
TTTCTCCAAGGATCGGCTTC+AGG 0.340930 1_4:+74871333 Msa0185030:CDS
TTGGAATTTGGCACTATAGA+TGG 0.341735 1_4:+74872946 Msa0185030:CDS
CAAGGATCGGCTTCAGGCCT+CGG 0.347948 1_4:+74871339 Msa0185030:CDS
TCCAGAGCTGTTTGGCCGTT+GGG 0.357398 1_4:-74872113 None:intergenic
CCGAACAAAGAAGAAGTATT+GGG 0.371136 1_4:+74871883 Msa0185030:CDS
TTCCTTTACCTCTTCCTGAT+TGG 0.374360 1_4:+74872927 Msa0185030:CDS
CCGATGCTCTTGCAAATATT+AGG 0.375111 1_4:+74871611 Msa0185030:CDS
GTTAAGCTCGGGAACTATAA+AGG 0.383225 1_4:+74871256 Msa0185030:CDS
TTTATTTATCTCCTAAGCTT+TGG 0.384714 1_4:+74871641 Msa0185030:CDS
TTTGCACCTCCAGCTTTGTC+AGG 0.385021 1_4:-74872381 None:intergenic
GGCTAATTTCTTCAGAATCA+TGG 0.388398 1_4:+74872767 Msa0185030:CDS
TCCTAAGCTTTGGTATTTGA+GGG 0.393359 1_4:+74871651 Msa0185030:CDS
CCTTTACGTGAGGGTTGTTA+AGG 0.393627 1_4:+74871180 Msa0185030:CDS
CAGCTCTGGAAATCTGGAAT+TGG 0.397726 1_4:+74872126 Msa0185030:CDS
GAGAGCCGAGTGTCCATGTT+AGG 0.399650 1_4:-74872984 None:intergenic
GGTTCGATCAAATTTGCAAC+TGG 0.404361 1_4:+74872823 Msa0185030:CDS
ACAAACAGTGCTGTTGCTCT+TGG 0.411446 1_4:-74873194 None:intergenic
CGTTGCTGCGGAACCATTTG+AGG 0.412932 1_4:+74871825 Msa0185030:CDS
CGAGGCCTGAAGCCGATCCT+TGG 0.414112 1_4:-74871338 None:intergenic
GCCCAACGGCCAAACAGCTC+TGG 0.415222 1_4:+74872112 Msa0185030:CDS
GGAATTGGTGTTCTTGAAGT+TGG 0.415516 1_4:+74872141 Msa0185030:CDS
TTTGAATGAGGTTCCGAAAC+GGG 0.416032 1_4:+74871429 Msa0185030:CDS
GAGAGTTAATGCTTGCTGTT+TGG 0.419766 1_4:+74871521 Msa0185030:CDS
TCGGATGCCGCGACTGTTAG+TGG 0.420415 1_4:+74871586 Msa0185030:CDS
TTGCCTTCAAAGGATGTTAC+CGG 0.421207 1_4:+74871211 Msa0185030:CDS
GGGTGTATATAGTGCATCTT+TGG 0.421466 1_4:-74872594 None:intergenic
TCTTTCAACAGAAAATCTTC+AGG 0.424219 1_4:-74871076 None:intergenic
AACCAATCCTGAATGGAATC+AGG 0.430195 1_4:+74871303 Msa0185030:CDS
AGACTTACAGCCAACTGATA+AGG 0.432910 1_4:+74871693 Msa0185030:CDS
TGTCAGAAGGCCGTGAAGTC+GGG 0.434203 1_4:-74873058 None:intergenic
GGCTGCAGTCTTTGCTGAGC+TGG 0.435790 1_4:+74873165 Msa0185030:CDS
AAAATCAGCGGCTCCTCAAA+TGG 0.439031 1_4:-74871838 None:intergenic
TTCCAGAGCTGTTTGGCCGT+TGG 0.439868 1_4:-74872114 None:intergenic
GTATAGATTGGAGGATAGAA+AGG 0.441691 1_4:+74871483 Msa0185030:CDS
TCGGCTTCAGGCCTCGGTGT+TGG 0.445911 1_4:+74871345 Msa0185030:CDS
CGCTGAGCCACCGCTGAGAA+AGG 0.446361 1_4:+74872686 Msa0185030:CDS
TCCAGATTTCCAGAGCTGTT+TGG 0.447417 1_4:-74872121 None:intergenic
AAGAAATTAAATTTGCAAGT+AGG 0.449686 1_4:+74872016 Msa0185030:CDS
AGTTGGGATATTGAGTGCTC+AGG 0.452833 1_4:+74872158 Msa0185030:CDS
CTTATTGTGTAGCGAAATAT+GGG 0.456005 1_4:+74872226 Msa0185030:CDS
GACTTACAGCCAACTGATAA+GGG 0.456275 1_4:+74871694 Msa0185030:CDS
GAATTGGTGTTCTTGAAGTT+GGG 0.456895 1_4:+74872142 Msa0185030:CDS
TTCGTCCGGATGAGCTGAAT+CGG 0.463142 1_4:-74873012 None:intergenic
AGAAAGGGTGATAAGGTTAA+GGG 0.464699 1_4:+74871499 Msa0185030:CDS
GGCGCCGCAATGGTATAGAT+TGG 0.464850 1_4:+74871471 Msa0185030:CDS
TGCTGTTTGGATGGGTACAC+AGG 0.468622 1_4:+74871534 Msa0185030:CDS
TCGACACATTCCCGACTTCA+CGG 0.468689 1_4:+74873048 Msa0185030:CDS
TATAGTGCCAAATTCCAATC+AGG 0.471795 1_4:-74872941 None:intergenic
CCTTGCACTGTGATTACAAT+TGG 0.473689 1_4:+74872330 Msa0185030:CDS
AACTGATAAGGGTAGATATC+CGG 0.474666 1_4:+74871705 Msa0185030:CDS
CGAGCTGGCTAACGGTCAAC+GGG 0.475727 1_4:-74872614 None:intergenic
AACTCTTCATCAAGTTCGTC+CGG 0.476174 1_4:-74873026 None:intergenic
ATTCAAGGATTGGTAAGGTA+AGG 0.476487 1_4:+74872406 Msa0185030:CDS
GAAAATGGGTGAAATTCAAT+TGG 0.477497 1_4:+74872509 Msa0185030:CDS
ACTGTTGGTAAGATAAGTTC+AGG 0.477704 1_4:-74872903 None:intergenic
GCTTCAGGCCTCGGTGTTGG+AGG 0.478715 1_4:+74871348 Msa0185030:CDS
GGAGGATAGAAAGGGTGATA+AGG 0.479059 1_4:+74871492 Msa0185030:CDS
GCTAATTTCTTCAGAATCAT+GGG 0.479725 1_4:+74872768 Msa0185030:CDS
ACCTAGTATTCACTGGTTTG+TGG 0.480851 1_4:-74871948 None:intergenic
AACCTGATTCCATTCAGGAT+TGG 0.483996 1_4:-74871305 None:intergenic
TTCCAATCAGGAAGAGGTAA+AGG 0.484097 1_4:-74872929 None:intergenic
GGCTAAGGACTTGCCTTCAA+AGG 0.484522 1_4:+74871201 Msa0185030:CDS
CGTGAGGGTTGTTAAGGCTA+AGG 0.486394 1_4:+74871186 Msa0185030:CDS
CTGGAGGTGCAAAAGATTCA+AGG 0.487820 1_4:+74872391 Msa0185030:CDS
ATGAAGCATTTCCAGAAGCT+TGG 0.489119 1_4:+74871560 Msa0185030:CDS
CGGTCAGAAGGGCCACAACT+TGG 0.491080 1_4:-74873262 None:intergenic
GCGGCGCCAAAGGACTATCT+GGG 0.491089 1_4:-74871456 None:intergenic
GTTGTGAAGGACGACTTCAT+TGG 0.491403 1_4:+74871391 Msa0185030:CDS
ACCACAAACCAGTGAATACT+AGG 0.491540 1_4:+74871947 Msa0185030:CDS
GTTGAGTATATGCTTGATGT+TGG 0.492238 1_4:+74872714 Msa0185030:CDS
ACCCTCAAATACCAAAGCTT+AGG 0.493243 1_4:-74871652 None:intergenic
AAAGAGACCAAACCCCATCT+TGG 0.497107 1_4:+74871094 Msa0185030:CDS
ATGTCTCAACACATAAATTC+CGG 0.498537 1_4:-74873282 None:intergenic
CTCAACAAGGTCATATGTGC+TGG 0.498939 1_4:-74871146 None:intergenic
TCCATGTTAGGAGGGTGTCT+TGG 0.501157 1_4:-74872972 None:intergenic
CAAGTTGTGGCCCTTCTGAC+CGG 0.503387 1_4:+74873263 Msa0185030:CDS
TCCGAACAAAGAAGAAGTAT+TGG 0.503767 1_4:+74871882 Msa0185030:CDS
GTTAATGCTTGCTGTTTGGA+TGG 0.505912 1_4:+74871525 Msa0185030:CDS
GGACTAGAATCTCTCTTAGC+AGG 0.511755 1_4:+74871767 Msa0185030:CDS
TTAGCCAGCTCGACAGTTTG+AGG 0.514684 1_4:+74872625 Msa0185030:CDS
ATGTCAGAAGGCCGTGAAGT+CGG 0.514758 1_4:-74873059 None:intergenic
AACAGAAAATCTTCAGGCGG+TGG 0.514836 1_4:-74871070 None:intergenic
AGAAGAAAACCAATCCTGAA+TGG 0.515488 1_4:+74871296 Msa0185030:CDS
GATCGACTTAGAAGCATAGC+TGG 0.518904 1_4:+74873098 Msa0185030:CDS
CTTCCGGTAACATCCTTTGA+AGG 0.519491 1_4:-74871214 None:intergenic
GGTGCAAAAGATTCAAGGAT+TGG 0.520817 1_4:+74872396 Msa0185030:CDS
TCGAGCTGGCTAACGGTCAA+CGG 0.520943 1_4:-74872615 None:intergenic
TCACGCCGATTCAGCTCATC+CGG 0.522054 1_4:+74873007 Msa0185030:CDS
GTTGGGATATTGAGTGCTCA+GGG 0.525396 1_4:+74872159 Msa0185030:CDS
CCTTAACAACCCTCACGTAA+AGG 0.525933 1_4:-74871180 None:intergenic
CACCACTTGGGTGAAGAACT+AGG 0.526449 1_4:-74872482 None:intergenic
TTAAACCACCTAGTATTCAC+TGG 0.527121 1_4:-74871955 None:intergenic
AGCCGAGTGTCCATGTTAGG+AGG 0.527424 1_4:-74872981 None:intergenic
TTGCATGGTCCTGACAAAGC+TGG 0.527750 1_4:+74872372 Msa0185030:CDS
GCCAAATTCCAATCAGGAAG+AGG 0.528138 1_4:-74872935 None:intergenic
TTTGAGGGTAAATGTGATTG+AGG 0.529044 1_4:+74871666 Msa0185030:CDS
ACACATAAATTCCGGTCAGA+AGG 0.532406 1_4:-74873274 None:intergenic
CTATACCATTGCGGCGCCAA+AGG 0.532688 1_4:-74871466 None:intergenic
AGCGTACGGATCACAACTTC+CGG 0.532790 1_4:-74871230 None:intergenic
AGCATGCAATCAGTTCTTGC+AGG 0.532832 1_4:-74873359 None:intergenic
TTCCTTTGCAGTTTATGGAT+AGG 0.533408 1_4:+74871917 Msa0185030:CDS
CCAAGTGGTGTGAAGAAAAT+GGG 0.533679 1_4:+74872495 Msa0185030:CDS
ATTTGAATGAGGTTCCGAAA+CGG 0.535448 1_4:+74871428 Msa0185030:CDS
TGCAAGTAGGATTCACATGA+GGG 0.537596 1_4:+74872029 Msa0185030:CDS
TTCTTCTCATACTCCACATG+TGG 0.540825 1_4:-74872740 None:intergenic
AAGAAATTAAGCGGCACCGA+CGG 0.541607 1_4:-74873326 None:intergenic
CACATAAATTCCGGTCAGAA+GGG 0.542177 1_4:-74873273 None:intergenic
AGAAGGGCCACAACTTGGAA+TGG 0.547838 1_4:-74873257 None:intergenic
GTGAAGAACTAGGAGTGGAT+AGG 0.551635 1_4:-74872472 None:intergenic
GCCAAGACACCCTCCTAACA+TGG 0.551812 1_4:+74872971 Msa0185030:CDS
CCGTACGCTGAAGTTAAGCT+CGG 0.557320 1_4:+74871244 Msa0185030:CDS
TGTGTGGTTTGATTTGAATG+AGG 0.557487 1_4:+74871417 Msa0185030:CDS
TGCCGCTTAATTTCTTCAGG+AGG 0.557905 1_4:+74873333 Msa0185030:CDS
GGATTTGATGTTCGTTGCTG+CGG 0.557925 1_4:+74871813 Msa0185030:CDS
TAGCGAAATATGGGCAGAAG+TGG 0.558621 1_4:+74872235 Msa0185030:CDS
AAAAGATTCAAGGATTGGTA+AGG 0.560457 1_4:+74872401 Msa0185030:CDS
CCTAATATTTGCAAGAGCAT+CGG 0.563372 1_4:-74871611 None:intergenic
AGACCAAACCCCATCTTGGA+GGG 0.563491 1_4:+74871098 Msa0185030:CDS
GAGACCAAACCCCATCTTGG+AGG 0.564193 1_4:+74871097 Msa0185030:CDS
ACGACTTCATTGGTCGTGTG+TGG 0.564266 1_4:+74871401 Msa0185030:CDS
TTTGATAATTGTCATTTGCA+TGG 0.564967 1_4:+74872357 Msa0185030:CDS
ATAGTCCTTTGGCGCCGCAA+TGG 0.565983 1_4:+74871461 Msa0185030:CDS
CTCCTATCCATAAACTGCAA+AGG 0.566217 1_4:-74871919 None:intergenic
GATGCCTCAAACTGTCGAGC+TGG 0.566280 1_4:-74872629 None:intergenic
ATTTCCAGAAGCTTGGCACT+CGG 0.566792 1_4:+74871567 Msa0185030:CDS
AAAACATATCATTGTAGAAG+GGG 0.567643 1_4:+74871984 Msa0185030:CDS
CCGAGCTTAACTTCAGCGTA+CGG 0.569596 1_4:-74871244 None:intergenic
GCCGCAATGGTATAGATTGG+AGG 0.570899 1_4:+74871474 Msa0185030:CDS
GGTGGAATGAGCAATATACT+TGG 0.572552 1_4:+74872295 Msa0185030:CDS
GGATATCTACCCTTATCAGT+TGG 0.576446 1_4:-74871703 None:intergenic
TATAGATTGGAGGATAGAAA+GGG 0.576864 1_4:+74871484 Msa0185030:CDS
TTGAGTGGACTAATTGCTGC+GGG 0.577897 1_4:+74872795 Msa0185030:CDS
CTCCTAGTTCTTCACCCAAG+TGG 0.579462 1_4:+74872480 Msa0185030:CDS
CAAACTGTCGAGCTGGCTAA+CGG 0.580833 1_4:-74872622 None:intergenic
TGTGACTCCATTCCAAGTTG+TGG 0.583105 1_4:+74873250 Msa0185030:CDS
ACTTAGCTACTCAAGGAGAA+AGG 0.584009 1_4:+74873144 Msa0185030:CDS
TTAATGCTTGCTGTTTGGAT+GGG 0.584454 1_4:+74871526 Msa0185030:CDS
ATCAAATCCTCGTTCCACAA+TGG 0.587304 1_4:-74871799 None:intergenic
ACCAAACCCCATCTTGGAGG+GGG 0.588344 1_4:+74871100 Msa0185030:CDS
TTGCAAGTAGGATTCACATG+AGG 0.591241 1_4:+74872028 Msa0185030:CDS
GGCAGAAGTGGATTCGAACA+AGG 0.592797 1_4:+74872247 Msa0185030:CDS
TTGATGTTGGTTCCCACATG+TGG 0.601847 1_4:+74872727 Msa0185030:CDS
AAGAGGTAAAGGAAAACTGT+TGG 0.606111 1_4:-74872918 None:intergenic
CCAATTGTAATCACAGTGCA+AGG 0.607117 1_4:-74872330 None:intergenic
ACTACCTCCTTTCTCAGCGG+TGG 0.608379 1_4:-74872693 None:intergenic
ACCCTCCTAACATGGACACT+CGG 0.608381 1_4:+74872979 Msa0185030:CDS
GGTAGGATACAGACTGTGGT+TGG 0.612469 1_4:+74873119 Msa0185030:CDS
CGTACGCTGAAGTTAAGCTC+GGG 0.613305 1_4:+74871245 Msa0185030:CDS
GACCAAACCCCATCTTGGAG+GGG 0.614269 1_4:+74871099 Msa0185030:CDS
CGGCGCCAAAGGACTATCTG+GGG 0.614676 1_4:-74871455 None:intergenic
GGAAGCTTATGACGGAACCT+CGG 0.615117 1_4:-74873305 None:intergenic
CTCATCCGAACAATGTCAGA+AGG 0.615248 1_4:-74873071 None:intergenic
GGAGGTTACTGTGAAAGATA+AGG 0.615500 1_4:+74871366 Msa0185030:CDS
TTCAACAGAAAATCTTCAGG+CGG 0.617196 1_4:-74871073 None:intergenic
AGAATCATGGGAGTATTGAG+TGG 0.619315 1_4:+74872780 Msa0185030:CDS
CAGCAGTGATCTTCGCCCAA+CGG 0.622948 1_4:+74872098 Msa0185030:CDS
GCCGAGTGTCCATGTTAGGA+GGG 0.626241 1_4:-74872980 None:intergenic
GAATTTGGCACTATAGATGG+AGG 0.626699 1_4:+74872949 Msa0185030:CDS
AGATGCAATACCTTTACGTG+AGG 0.626728 1_4:+74871170 Msa0185030:CDS
ATGAGGGTTTGTCTAGAAGG+TGG 0.627125 1_4:+74872045 Msa0185030:CDS
GTGGAATGAGCAATATACTT+GGG 0.627653 1_4:+74872296 Msa0185030:CDS
CTTGGGTGAAGAACTAGGAG+TGG 0.630605 1_4:-74872477 None:intergenic
TGAGCCACCGCTGAGAAAGG+AGG 0.630758 1_4:+74872689 Msa0185030:CDS
GACTTAGAAGCATAGCTGGT+AGG 0.632636 1_4:+74873102 Msa0185030:CDS
GGTATTGCATCTGCTCAACA+AGG 0.633340 1_4:-74871159 None:intergenic
TAGTGCATCTTTGGCAACAG+AGG 0.634644 1_4:-74872585 None:intergenic
GATGCAATACCTTTACGTGA+GGG 0.638362 1_4:+74871171 Msa0185030:CDS
GCACCGACGGAAGCTTATGA+CGG 0.638901 1_4:-74873313 None:intergenic
TTGATAGCTTTATGCCGAGG+TGG 0.644610 1_4:+74872277 Msa0185030:CDS
TCCTCCAATCTATACCATTG+CGG 0.645041 1_4:-74871475 None:intergenic
GAATGAGCAATATACTTGGG+AGG 0.645094 1_4:+74872299 Msa0185030:CDS
GAAAGATAAGGATGTTGTGA+AGG 0.645624 1_4:+74871378 Msa0185030:CDS
GTTGGCGACTTAGCTACTCA+AGG 0.648005 1_4:+74873137 Msa0185030:CDS
GCAGGAGTATCAATCCATTG+TGG 0.649222 1_4:+74871785 Msa0185030:CDS
GTGGAGTATGAGAAGAAGCA+AGG 0.650662 1_4:+74872746 Msa0185030:CDS
ATCGGTTCCACTAACAGTCG+CGG 0.651678 1_4:-74871593 None:intergenic
AGCCTCCTGAAGAAATTAAG+CGG 0.651985 1_4:-74873335 None:intergenic
GTTCCGTCATAAGCTTCCGT+CGG 0.653799 1_4:+74873310 Msa0185030:CDS
GTGAAATTCAATTGGCTGTG+AGG 0.654400 1_4:+74872517 Msa0185030:CDS
GGAACTATAAAGGAACTACT+CGG 0.658774 1_4:+74871266 Msa0185030:CDS
ATTGAGTGGACTAATTGCTG+CGG 0.661101 1_4:+74872794 Msa0185030:CDS
CTACTCAGATAGTTTCAATG+AGG 0.663957 1_4:+74872655 Msa0185030:CDS
TCATTGATAGCTTTATGCCG+AGG 0.666881 1_4:+74872274 Msa0185030:CDS
TATCAATCCATTGTGGAACG+AGG 0.672925 1_4:+74871792 Msa0185030:CDS
GGCGCCAAAGGACTATCTGG+GGG 0.676642 1_4:-74871454 None:intergenic
CGAACAAAGAAGAAGTATTG+GGG 0.685152 1_4:+74871884 Msa0185030:CDS
TCTTCCACACTCAAAATCAG+CGG 0.687134 1_4:-74871850 None:intergenic
TCTTCTCATACTCCACATGT+GGG 0.689277 1_4:-74872739 None:intergenic
TTTATGTGTTGAGACATCCG+AGG 0.696759 1_4:+74873288 Msa0185030:CDS
AGCTGGTAGGATACAGACTG+TGG 0.703110 1_4:+74873115 Msa0185030:CDS
ACAAAGAAGAAGTATTGGGG+AGG 0.704686 1_4:+74871887 Msa0185030:CDS
CATGGTCCTGACAAAGCTGG+AGG 0.704809 1_4:+74872375 Msa0185030:CDS
GTATATTGCTCATTCCACCT+CGG 0.716433 1_4:-74872291 None:intergenic
TCAACTACCTCCTTTCTCAG+CGG 0.739237 1_4:-74872696 None:intergenic
CACAGTAACCTCCAACACCG+AGG 0.741552 1_4:-74871356 None:intergenic
ACAAACCAGTGAATACTAGG+TGG 0.765072 1_4:+74871950 Msa0185030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTTTAAATTATTAT+CGG + chr1_4:74870835-74870854 Msa0185030:intron 0.0%
!!! ATTTTTTTATTTATGTAACA+CGG + chr1_4:74870648-74870667 Msa0185030:intron 10.0%
!!! ATTAATTTTCACAAAATTTT+AGG + chr1_4:74870904-74870923 Msa0185030:intron 10.0%
!! AATAAAATGTAACATTTCAA+AGG - chr1_4:74870323-74870342 None:intergenic 15.0%
!! ACTAAAAAAATATACAAACA+AGG - chr1_4:74870607-74870626 None:intergenic 15.0%
!!! TGTTATATTGTTGTTTTTTT+AGG + chr1_4:74870962-74870981 Msa0185030:intron 15.0%
!!! ATTTTTTGTTTATTACTTGC+AGG + chr1_4:74870431-74870450 Msa0185030:intron 20.0%
!!! AAATTTTAGGTGTTTAGATT+TGG + chr1_4:74870917-74870936 Msa0185030:intron 20.0%
!! AAAAACATATCATTGTAGAA+GGG + chr1_4:74871983-74872002 Msa0185030:CDS 20.0%
!! AAGAAATTAAATTTGCAAGT+AGG + chr1_4:74872016-74872035 Msa0185030:CDS 20.0%
!! TCATATCTTGTTCATAATAT+TGG + chr1_4:74872872-74872891 Msa0185030:CDS 20.0%
! TTTATTTATCTCCTAAGCTT+TGG + chr1_4:74871641-74871660 Msa0185030:CDS 25.0%
! GAAAAACATATCATTGTAGA+AGG + chr1_4:74871982-74872001 Msa0185030:CDS 25.0%
! AAAACATATCATTGTAGAAG+GGG + chr1_4:74871984-74872003 Msa0185030:CDS 25.0%
! ATGCCAATGAAAAATAAAGA+TGG + chr1_4:74872186-74872205 Msa0185030:CDS 25.0%
! TGCCAATGAAAAATAAAGAT+GGG + chr1_4:74872187-74872206 Msa0185030:CDS 25.0%
! AATGAAAAATAAAGATGGGA+GGG + chr1_4:74872191-74872210 Msa0185030:CDS 25.0%
!! TTTGATAATTGTCATTTGCA+TGG + chr1_4:74872357-74872376 Msa0185030:CDS 25.0%
AAACCAAAAGCTTTCTTTCA+AGG - chr1_4:74870477-74870496 None:intergenic 30.0%
!! TTGAAAGAAAGCTTTTGGTT+TGG + chr1_4:74870476-74870495 Msa0185030:intron 30.0%
TTAGATTTGGCAACTTGTAA+TGG + chr1_4:74870930-74870949 Msa0185030:intron 30.0%
!!! TTTTTTTAGGCTTCTGAAGT+TGG + chr1_4:74870975-74870994 Msa0185030:intron 30.0%
TCTTTCAACAGAAAATCTTC+AGG - chr1_4:74871079-74871098 None:intergenic 30.0%
TATAGATTGGAGGATAGAAA+GGG + chr1_4:74871484-74871503 Msa0185030:CDS 30.0%
! AGTTTTTGTGAAGGCTATTT+TGG + chr1_4:74871729-74871748 Msa0185030:CDS 30.0%
!! GTTTTTGTGAAGGCTATTTT+GGG + chr1_4:74871730-74871749 Msa0185030:CDS 30.0%
!! TTTTTGTGAAGGCTATTTTG+GGG + chr1_4:74871731-74871750 Msa0185030:CDS 30.0%
!!! CTCCCATCTTTATTTTTCAT+TGG - chr1_4:74872192-74872211 None:intergenic 30.0%
CAATGAAAAATAAAGATGGG+AGG + chr1_4:74872190-74872209 Msa0185030:CDS 30.0%
ATGAAAAATAAAGATGGGAG+GGG + chr1_4:74872192-74872211 Msa0185030:CDS 30.0%
CTTATTGTGTAGCGAAATAT+GGG + chr1_4:74872226-74872245 Msa0185030:CDS 30.0%
! AAAAGATTCAAGGATTGGTA+AGG + chr1_4:74872401-74872420 Msa0185030:CDS 30.0%
GAAAATGGGTGAAATTCAAT+TGG + chr1_4:74872509-74872528 Msa0185030:CDS 30.0%
GCTAATTTCTTCAGAATCAT+GGG + chr1_4:74872768-74872787 Msa0185030:CDS 30.0%
TGAATCAGAACTGTTGTAAT+TGG - chr1_4:74872855-74872874 None:intergenic 30.0%
ATGTCTCAACACATAAATTC+CGG - chr1_4:74873285-74873304 None:intergenic 30.0%
!! AACTCTGAAATCAGAGATCT+AGG + chr1_4:74870499-74870518 Msa0185030:intron 35.0%
!! CAGCAACATCTTAGTTTTTC+TGG + chr1_4:74871007-74871026 Msa0185030:intron 35.0%
TTCAACAGAAAATCTTCAGG+CGG - chr1_4:74871076-74871095 None:intergenic 35.0%
GGAACTATAAAGGAACTACT+CGG + chr1_4:74871266-74871285 Msa0185030:CDS 35.0%
AGAAGAAAACCAATCCTGAA+TGG + chr1_4:74871296-74871315 Msa0185030:CDS 35.0%
! TTTTTGCTTTCTCCAAGGAT+CGG + chr1_4:74871326-74871345 Msa0185030:CDS 35.0%
GAAAGATAAGGATGTTGTGA+AGG + chr1_4:74871378-74871397 Msa0185030:CDS 35.0%
!! TGTGTGGTTTGATTTGAATG+AGG + chr1_4:74871417-74871436 Msa0185030:CDS 35.0%
ATTTGAATGAGGTTCCGAAA+CGG + chr1_4:74871428-74871447 Msa0185030:CDS 35.0%
GTATAGATTGGAGGATAGAA+AGG + chr1_4:74871483-74871502 Msa0185030:CDS 35.0%
TAGAAAGGGTGATAAGGTTA+AGG + chr1_4:74871498-74871517 Msa0185030:CDS 35.0%
AGAAAGGGTGATAAGGTTAA+GGG + chr1_4:74871499-74871518 Msa0185030:CDS 35.0%
!! TTAATGCTTGCTGTTTGGAT+GGG + chr1_4:74871526-74871545 Msa0185030:CDS 35.0%
! CCTAATATTTGCAAGAGCAT+CGG - chr1_4:74871614-74871633 None:intergenic 35.0%
! TCCTAAGCTTTGGTATTTGA+GGG + chr1_4:74871651-74871670 Msa0185030:CDS 35.0%
TTTGAGGGTAAATGTGATTG+AGG + chr1_4:74871666-74871685 Msa0185030:CDS 35.0%
AACTGATAAGGGTAGATATC+CGG + chr1_4:74871705-74871724 Msa0185030:CDS 35.0%
! ATATCCGGAAGTTTTTGTGA+AGG + chr1_4:74871720-74871739 Msa0185030:CDS 35.0%
ATAGCCTTCACAAAAACTTC+CGG - chr1_4:74871727-74871746 None:intergenic 35.0%
! TCCGAACAAAGAAGAAGTAT+TGG + chr1_4:74871882-74871901 Msa0185030:CDS 35.0%
CCCAATACTTCTTCTTTGTT+CGG - chr1_4:74871886-74871905 None:intergenic 35.0%
!! CCGAACAAAGAAGAAGTATT+GGG + chr1_4:74871883-74871902 Msa0185030:CDS 35.0%
!! CGAACAAAGAAGAAGTATTG+GGG + chr1_4:74871884-74871903 Msa0185030:CDS 35.0%
TGCAATTCCTTTGCAGTTTA+TGG + chr1_4:74871912-74871931 Msa0185030:CDS 35.0%
TTCCTTTGCAGTTTATGGAT+AGG + chr1_4:74871917-74871936 Msa0185030:CDS 35.0%
TTAAACCACCTAGTATTCAC+TGG - chr1_4:74871958-74871977 None:intergenic 35.0%
! AGAAGGTGGTTATCATGTTT+TGG + chr1_4:74872059-74872078 Msa0185030:CDS 35.0%
! GAATTGGTGTTCTTGAAGTT+GGG + chr1_4:74872142-74872161 Msa0185030:CDS 35.0%
TGAAAAATAAAGATGGGAGG+GGG + chr1_4:74872193-74872212 Msa0185030:CDS 35.0%
GCTTATTGTGTAGCGAAATA+TGG + chr1_4:74872225-74872244 Msa0185030:CDS 35.0%
GTGGAATGAGCAATATACTT+GGG + chr1_4:74872296-74872315 Msa0185030:CDS 35.0%
! ATTCAAGGATTGGTAAGGTA+AGG + chr1_4:74872406-74872425 Msa0185030:CDS 35.0%
CTACTCAGATAGTTTCAATG+AGG + chr1_4:74872655-74872674 Msa0185030:CDS 35.0%
GTTGAGTATATGCTTGATGT+TGG + chr1_4:74872714-74872733 Msa0185030:CDS 35.0%
GGCTAATTTCTTCAGAATCA+TGG + chr1_4:74872767-74872786 Msa0185030:CDS 35.0%
! ACTGTTGGTAAGATAAGTTC+AGG - chr1_4:74872906-74872925 None:intergenic 35.0%
AAGAGGTAAAGGAAAACTGT+TGG - chr1_4:74872921-74872940 None:intergenic 35.0%
TATAGTGCCAAATTCCAATC+AGG - chr1_4:74872944-74872963 None:intergenic 35.0%
TTGGAATTTGGCACTATAGA+TGG + chr1_4:74872946-74872965 Msa0185030:CDS 35.0%
! GTGCCTTGAAAGAAAGCTTT+TGG + chr1_4:74870471-74870490 Msa0185030:intron 40.0%
TCACACTTCGACATGATACT+GGG + chr1_4:74870752-74870771 Msa0185030:intron 40.0%
TATGTGTCAGGTGTTTGTGT+CGG + chr1_4:74870868-74870887 Msa0185030:intron 40.0%
TTTGAGCAACGAAAATGCAG+AGG + chr1_4:74871047-74871066 Msa0185030:intron 40.0%
AGATGCAATACCTTTACGTG+AGG + chr1_4:74871170-74871189 Msa0185030:CDS 40.0%
GATGCAATACCTTTACGTGA+GGG + chr1_4:74871171-74871190 Msa0185030:CDS 40.0%
TTGCCTTCAAAGGATGTTAC+CGG + chr1_4:74871211-74871230 Msa0185030:CDS 40.0%
GTTAAGCTCGGGAACTATAA+AGG + chr1_4:74871256-74871275 Msa0185030:CDS 40.0%
AACCAATCCTGAATGGAATC+AGG + chr1_4:74871303-74871322 Msa0185030:CDS 40.0%
AACCTGATTCCATTCAGGAT+TGG - chr1_4:74871308-74871327 None:intergenic 40.0%
GCAAAAACCTGATTCCATTC+AGG - chr1_4:74871313-74871332 None:intergenic 40.0%
! TCAGGTTTTTGCTTTCTCCA+AGG + chr1_4:74871321-74871340 Msa0185030:CDS 40.0%
GGAGGTTACTGTGAAAGATA+AGG + chr1_4:74871366-74871385 Msa0185030:CDS 40.0%
TTTGAATGAGGTTCCGAAAC+GGG + chr1_4:74871429-74871448 Msa0185030:CDS 40.0%
TCCTCCAATCTATACCATTG+CGG - chr1_4:74871478-74871497 None:intergenic 40.0%
!! GAGAGTTAATGCTTGCTGTT+TGG + chr1_4:74871521-74871540 Msa0185030:CDS 40.0%
!! GTTAATGCTTGCTGTTTGGA+TGG + chr1_4:74871525-74871544 Msa0185030:CDS 40.0%
! ATGAAGCATTTCCAGAAGCT+TGG + chr1_4:74871560-74871579 Msa0185030:CDS 40.0%
CCGATGCTCTTGCAAATATT+AGG + chr1_4:74871611-74871630 Msa0185030:CDS 40.0%
! CTCCTAAGCTTTGGTATTTG+AGG + chr1_4:74871650-74871669 Msa0185030:CDS 40.0%
ACCCTCAAATACCAAAGCTT+AGG - chr1_4:74871655-74871674 None:intergenic 40.0%
AGACTTACAGCCAACTGATA+AGG + chr1_4:74871693-74871712 Msa0185030:CDS 40.0%
GACTTACAGCCAACTGATAA+GGG + chr1_4:74871694-74871713 Msa0185030:CDS 40.0%
GGATATCTACCCTTATCAGT+TGG - chr1_4:74871706-74871725 None:intergenic 40.0%
TATCAATCCATTGTGGAACG+AGG + chr1_4:74871792-74871811 Msa0185030:CDS 40.0%
ATCAAATCCTCGTTCCACAA+TGG - chr1_4:74871802-74871821 None:intergenic 40.0%
TCTTCCACACTCAAAATCAG+CGG - chr1_4:74871853-74871872 None:intergenic 40.0%
!! ACAAAGAAGAAGTATTGGGG+AGG + chr1_4:74871887-74871906 Msa0185030:CDS 40.0%
CTCCTATCCATAAACTGCAA+AGG - chr1_4:74871922-74871941 None:intergenic 40.0%
ACCACAAACCAGTGAATACT+AGG + chr1_4:74871947-74871966 Msa0185030:CDS 40.0%
!! ACCTAGTATTCACTGGTTTG+TGG - chr1_4:74871951-74871970 None:intergenic 40.0%
ACAAACCAGTGAATACTAGG+TGG + chr1_4:74871950-74871969 Msa0185030:CDS 40.0%
TTGCAAGTAGGATTCACATG+AGG + chr1_4:74872028-74872047 Msa0185030:CDS 40.0%
TGCAAGTAGGATTCACATGA+GGG + chr1_4:74872029-74872048 Msa0185030:CDS 40.0%
! GGAATTGGTGTTCTTGAAGT+TGG + chr1_4:74872141-74872160 Msa0185030:CDS 40.0%
!! TCATTGATAGCTTTATGCCG+AGG + chr1_4:74872274-74872293 Msa0185030:CDS 40.0%
GTATATTGCTCATTCCACCT+CGG - chr1_4:74872294-74872313 None:intergenic 40.0%
GGTGGAATGAGCAATATACT+TGG + chr1_4:74872295-74872314 Msa0185030:CDS 40.0%
GAATGAGCAATATACTTGGG+AGG + chr1_4:74872299-74872318 Msa0185030:CDS 40.0%
CCAATTGTAATCACAGTGCA+AGG - chr1_4:74872333-74872352 None:intergenic 40.0%
CCTTGCACTGTGATTACAAT+TGG + chr1_4:74872330-74872349 Msa0185030:CDS 40.0%
GGTGCAAAAGATTCAAGGAT+TGG + chr1_4:74872396-74872415 Msa0185030:CDS 40.0%
! CCATTTTCTTCACACCACTT+GGG - chr1_4:74872497-74872516 None:intergenic 40.0%
CCAAGTGGTGTGAAGAAAAT+GGG + chr1_4:74872495-74872514 Msa0185030:CDS 40.0%
GTGAAATTCAATTGGCTGTG+AGG + chr1_4:74872517-74872536 Msa0185030:CDS 40.0%
GGGTGTATATAGTGCATCTT+TGG - chr1_4:74872597-74872616 None:intergenic 40.0%
TCTTCTCATACTCCACATGT+GGG - chr1_4:74872742-74872761 None:intergenic 40.0%
TTCTTCTCATACTCCACATG+TGG - chr1_4:74872743-74872762 None:intergenic 40.0%
!! AGAATCATGGGAGTATTGAG+TGG + chr1_4:74872780-74872799 Msa0185030:CDS 40.0%
!! ATTGAGTGGACTAATTGCTG+CGG + chr1_4:74872794-74872813 Msa0185030:CDS 40.0%
GGTTCGATCAAATTTGCAAC+TGG + chr1_4:74872823-74872842 Msa0185030:CDS 40.0%
TTCCTTTACCTCTTCCTGAT+TGG + chr1_4:74872927-74872946 Msa0185030:CDS 40.0%
TTCCAATCAGGAAGAGGTAA+AGG - chr1_4:74872932-74872951 None:intergenic 40.0%
ACCTCTTCCTGATTGGAATT+TGG + chr1_4:74872934-74872953 Msa0185030:CDS 40.0%
GAATTTGGCACTATAGATGG+AGG + chr1_4:74872949-74872968 Msa0185030:CDS 40.0%
AACTCTTCATCAAGTTCGTC+CGG - chr1_4:74873029-74873048 None:intergenic 40.0%
ACTTAGCTACTCAAGGAGAA+AGG + chr1_4:74873144-74873163 Msa0185030:CDS 40.0%
CACATAAATTCCGGTCAGAA+GGG - chr1_4:74873276-74873295 None:intergenic 40.0%
ACACATAAATTCCGGTCAGA+AGG - chr1_4:74873277-74873296 None:intergenic 40.0%
TTTATGTGTTGAGACATCCG+AGG + chr1_4:74873288-74873307 Msa0185030:CDS 40.0%
AGCCTCCTGAAGAAATTAAG+CGG - chr1_4:74873338-74873357 None:intergenic 40.0%
ACACAGACACTTCAGATCGT+AGG + chr1_4:74870711-74870730 Msa0185030:intron 45.0%
GTCACACTTCGACATGATAC+TGG + chr1_4:74870751-74870770 Msa0185030:intron 45.0%
GGTGTCTACATGTATGTGTC+AGG + chr1_4:74870856-74870875 Msa0185030:intron 45.0%
AACAGAAAATCTTCAGGCGG+TGG - chr1_4:74871073-74871092 None:intergenic 45.0%
AAAGAGACCAAACCCCATCT+TGG + chr1_4:74871094-74871113 Msa0185030:CDS 45.0%
! ATTTTTCCCCCTCCAAGATG+GGG - chr1_4:74871109-74871128 None:intergenic 45.0%
! GATTTTTCCCCCTCCAAGAT+GGG - chr1_4:74871110-74871129 None:intergenic 45.0%
! AGATTTTTCCCCCTCCAAGA+TGG - chr1_4:74871111-74871130 None:intergenic 45.0%
CTCAACAAGGTCATATGTGC+TGG - chr1_4:74871149-74871168 None:intergenic 45.0%
GGTATTGCATCTGCTCAACA+AGG - chr1_4:74871162-74871181 None:intergenic 45.0%
CCTTAACAACCCTCACGTAA+AGG - chr1_4:74871183-74871202 None:intergenic 45.0%
CCTTTACGTGAGGGTTGTTA+AGG + chr1_4:74871180-74871199 Msa0185030:CDS 45.0%
CTTCCGGTAACATCCTTTGA+AGG - chr1_4:74871217-74871236 None:intergenic 45.0%
GTTGTGAAGGACGACTTCAT+TGG + chr1_4:74871391-74871410 Msa0185030:CDS 45.0%
GGAGGATAGAAAGGGTGATA+AGG + chr1_4:74871492-74871511 Msa0185030:CDS 45.0%
ATTTCCAGAAGCTTGGCACT+CGG + chr1_4:74871567-74871586 Msa0185030:CDS 45.0%
!! GAAGGCTATTTTGGGGAATC+AGG + chr1_4:74871738-74871757 Msa0185030:CDS 45.0%
!! TTTTGGGGAATCAGGCATTG+AGG + chr1_4:74871746-74871765 Msa0185030:CDS 45.0%
GGACTAGAATCTCTCTTAGC+AGG + chr1_4:74871767-74871786 Msa0185030:CDS 45.0%
!! GCAGGAGTATCAATCCATTG+TGG + chr1_4:74871785-74871804 Msa0185030:CDS 45.0%
GGATTTGATGTTCGTTGCTG+CGG + chr1_4:74871813-74871832 Msa0185030:CDS 45.0%
AAAATCAGCGGCTCCTCAAA+TGG - chr1_4:74871841-74871860 None:intergenic 45.0%
CACATGAGGGTTTGTCTAGA+AGG + chr1_4:74872042-74872061 Msa0185030:CDS 45.0%
ATGAGGGTTTGTCTAGAAGG+TGG + chr1_4:74872045-74872064 Msa0185030:CDS 45.0%
TCCAGATTTCCAGAGCTGTT+TGG - chr1_4:74872124-74872143 None:intergenic 45.0%
!! CAGCTCTGGAAATCTGGAAT+TGG + chr1_4:74872126-74872145 Msa0185030:CDS 45.0%
AGTTGGGATATTGAGTGCTC+AGG + chr1_4:74872158-74872177 Msa0185030:CDS 45.0%
GTTGGGATATTGAGTGCTCA+GGG + chr1_4:74872159-74872178 Msa0185030:CDS 45.0%
TAGCGAAATATGGGCAGAAG+TGG + chr1_4:74872235-74872254 Msa0185030:CDS 45.0%
!! TTGATAGCTTTATGCCGAGG+TGG + chr1_4:74872277-74872296 Msa0185030:CDS 45.0%
! CTGGAGGTGCAAAAGATTCA+AGG + chr1_4:74872391-74872410 Msa0185030:CDS 45.0%
GTGAAGAACTAGGAGTGGAT+AGG - chr1_4:74872475-74872494 None:intergenic 45.0%
CCCAAGTGGTGTGAAGAAAA+TGG + chr1_4:74872494-74872513 Msa0185030:CDS 45.0%
! CCCATTTTCTTCACACCACT+TGG - chr1_4:74872498-74872517 None:intergenic 45.0%
TAGTGCATCTTTGGCAACAG+AGG - chr1_4:74872588-74872607 None:intergenic 45.0%
TCAACTACCTCCTTTCTCAG+CGG - chr1_4:74872699-74872718 None:intergenic 45.0%
!! TTGATGTTGGTTCCCACATG+TGG + chr1_4:74872727-74872746 Msa0185030:CDS 45.0%
GTGGAGTATGAGAAGAAGCA+AGG + chr1_4:74872746-74872765 Msa0185030:CDS 45.0%
!! TTGAGTGGACTAATTGCTGC+GGG + chr1_4:74872795-74872814 Msa0185030:CDS 45.0%
GACTAATTGCTGCGGGAAAA+TGG + chr1_4:74872802-74872821 Msa0185030:CDS 45.0%
GCCAAATTCCAATCAGGAAG+AGG - chr1_4:74872938-74872957 None:intergenic 45.0%
CTCATCCGAACAATGTCAGA+AGG - chr1_4:74873074-74873093 None:intergenic 45.0%
! GATCGACTTAGAAGCATAGC+TGG + chr1_4:74873098-74873117 Msa0185030:CDS 45.0%
! GACTTAGAAGCATAGCTGGT+AGG + chr1_4:74873102-74873121 Msa0185030:CDS 45.0%
! ACAAACAGTGCTGTTGCTCT+TGG - chr1_4:74873197-74873216 None:intergenic 45.0%
TGTGACTCCATTCCAAGTTG+TGG + chr1_4:74873250-74873269 Msa0185030:CDS 45.0%
! AAGAAATTAAGCGGCACCGA+CGG - chr1_4:74873329-74873348 None:intergenic 45.0%
! TGCCGCTTAATTTCTTCAGG+AGG + chr1_4:74873333-74873352 Msa0185030:CDS 45.0%
AGCATGCAATCAGTTCTTGC+AGG - chr1_4:74873362-74873381 None:intergenic 45.0%
TCAGATCGTAGGTGTGTGTC+CGG + chr1_4:74870722-74870741 Msa0185030:intron 50.0%
GTCGAAGTGTGACACCATAC+CGG - chr1_4:74870744-74870763 None:intergenic 50.0%
AGACCAAACCCCATCTTGGA+GGG + chr1_4:74871098-74871117 Msa0185030:CDS 50.0%
CTTGGAGGGGGAAAAATCTC+TGG + chr1_4:74871112-74871131 Msa0185030:CDS 50.0%
CGTGAGGGTTGTTAAGGCTA+AGG + chr1_4:74871186-74871205 Msa0185030:CDS 50.0%
GGCTAAGGACTTGCCTTCAA+AGG + chr1_4:74871201-74871220 Msa0185030:CDS 50.0%
AGCGTACGGATCACAACTTC+CGG - chr1_4:74871233-74871252 None:intergenic 50.0%
CCGAGCTTAACTTCAGCGTA+CGG - chr1_4:74871247-74871266 None:intergenic 50.0%
CCGTACGCTGAAGTTAAGCT+CGG + chr1_4:74871244-74871263 Msa0185030:CDS 50.0%
CGTACGCTGAAGTTAAGCTC+GGG + chr1_4:74871245-74871264 Msa0185030:CDS 50.0%
! TTTCTCCAAGGATCGGCTTC+AGG + chr1_4:74871333-74871352 Msa0185030:CDS 50.0%
ACGACTTCATTGGTCGTGTG+TGG + chr1_4:74871401-74871420 Msa0185030:CDS 50.0%
GCCGCAATGGTATAGATTGG+AGG + chr1_4:74871474-74871493 Msa0185030:CDS 50.0%
! TGCTGTTTGGATGGGTACAC+AGG + chr1_4:74871534-74871553 Msa0185030:CDS 50.0%
! ATCGGTTCCACTAACAGTCG+CGG - chr1_4:74871596-74871615 None:intergenic 50.0%
!! GCCAAACAGCTCTGGAAATC+TGG + chr1_4:74872120-74872139 Msa0185030:CDS 50.0%
! GGCAGAAGTGGATTCGAACA+AGG + chr1_4:74872247-74872266 Msa0185030:CDS 50.0%
TTGCATGGTCCTGACAAAGC+TGG + chr1_4:74872372-74872391 Msa0185030:CDS 50.0%
! TTTGCACCTCCAGCTTTGTC+AGG - chr1_4:74872384-74872403 None:intergenic 50.0%
CTTGGGTGAAGAACTAGGAG+TGG - chr1_4:74872480-74872499 None:intergenic 50.0%
! CTCCTAGTTCTTCACCCAAG+TGG + chr1_4:74872480-74872499 Msa0185030:CDS 50.0%
CACCACTTGGGTGAAGAACT+AGG - chr1_4:74872485-74872504 None:intergenic 50.0%
CAAACTGTCGAGCTGGCTAA+CGG - chr1_4:74872625-74872644 None:intergenic 50.0%
TTAGCCAGCTCGACAGTTTG+AGG + chr1_4:74872625-74872644 Msa0185030:CDS 50.0%
!! TCCATGTTAGGAGGGTGTCT+TGG - chr1_4:74872975-74872994 None:intergenic 50.0%
ACCCTCCTAACATGGACACT+CGG + chr1_4:74872979-74872998 Msa0185030:CDS 50.0%
TTCGTCCGGATGAGCTGAAT+CGG - chr1_4:74873015-74873034 None:intergenic 50.0%
TCGACACATTCCCGACTTCA+CGG + chr1_4:74873048-74873067 Msa0185030:CDS 50.0%
ATGTCAGAAGGCCGTGAAGT+CGG - chr1_4:74873062-74873081 None:intergenic 50.0%
CACGGCCTTCTGACATTGTT+CGG + chr1_4:74873066-74873085 Msa0185030:CDS 50.0%
AGCTGGTAGGATACAGACTG+TGG + chr1_4:74873115-74873134 Msa0185030:CDS 50.0%
GGTAGGATACAGACTGTGGT+TGG + chr1_4:74873119-74873138 Msa0185030:CDS 50.0%
GTTGGCGACTTAGCTACTCA+AGG + chr1_4:74873137-74873156 Msa0185030:CDS 50.0%
AGAAGGGCCACAACTTGGAA+TGG - chr1_4:74873260-74873279 None:intergenic 50.0%
! GGAAGCTTATGACGGAACCT+CGG - chr1_4:74873308-74873327 None:intergenic 50.0%
GTTCCGTCATAAGCTTCCGT+CGG + chr1_4:74873310-74873329 Msa0185030:CDS 50.0%
!! CGGTGCCGCTTAATTTCTTC+AGG + chr1_4:74873330-74873349 Msa0185030:CDS 50.0%
TCGTAGGTGTGTGTCCGGTA+TGG + chr1_4:74870727-74870746 Msa0185030:intron 55.0%
GAGACCAAACCCCATCTTGG+AGG + chr1_4:74871097-74871116 Msa0185030:CDS 55.0%
GACCAAACCCCATCTTGGAG+GGG + chr1_4:74871099-74871118 Msa0185030:CDS 55.0%
ACCAAACCCCATCTTGGAGG+GGG + chr1_4:74871100-74871119 Msa0185030:CDS 55.0%
CACAGTAACCTCCAACACCG+AGG - chr1_4:74871359-74871378 None:intergenic 55.0%
CTATCTGGGGGAACCCGTTT+CGG - chr1_4:74871445-74871464 None:intergenic 55.0%
GGTTCCCCCAGATAGTCCTT+TGG + chr1_4:74871450-74871469 Msa0185030:CDS 55.0%
ATAGTCCTTTGGCGCCGCAA+TGG + chr1_4:74871461-74871480 Msa0185030:CDS 55.0%
CTATACCATTGCGGCGCCAA+AGG - chr1_4:74871469-74871488 None:intergenic 55.0%
GGCGCCGCAATGGTATAGAT+TGG + chr1_4:74871471-74871490 Msa0185030:CDS 55.0%
CGTTGCTGCGGAACCATTTG+AGG + chr1_4:74871825-74871844 Msa0185030:CDS 55.0%
!!! GGAGCCGCTGATTTTGAGTG+TGG + chr1_4:74871846-74871865 Msa0185030:CDS 55.0%
CAGCAGTGATCTTCGCCCAA+CGG + chr1_4:74872098-74872117 Msa0185030:CDS 55.0%
TCCAGAGCTGTTTGGCCGTT+GGG - chr1_4:74872116-74872135 None:intergenic 55.0%
TTCCAGAGCTGTTTGGCCGT+TGG - chr1_4:74872117-74872136 None:intergenic 55.0%
CATGGTCCTGACAAAGCTGG+AGG + chr1_4:74872375-74872394 Msa0185030:CDS 55.0%
TCGAGCTGGCTAACGGTCAA+CGG - chr1_4:74872618-74872637 None:intergenic 55.0%
GATGCCTCAAACTGTCGAGC+TGG - chr1_4:74872632-74872651 None:intergenic 55.0%
ACTACCTCCTTTCTCAGCGG+TGG - chr1_4:74872696-74872715 None:intergenic 55.0%
GCCAAGACACCCTCCTAACA+TGG + chr1_4:74872971-74872990 Msa0185030:CDS 55.0%
GCCGAGTGTCCATGTTAGGA+GGG - chr1_4:74872983-74873002 None:intergenic 55.0%
AGCCGAGTGTCCATGTTAGG+AGG - chr1_4:74872984-74873003 None:intergenic 55.0%
GAGAGCCGAGTGTCCATGTT+AGG - chr1_4:74872987-74873006 None:intergenic 55.0%
!! TCACGCCGATTCAGCTCATC+CGG + chr1_4:74873007-74873026 Msa0185030:CDS 55.0%
TGTCAGAAGGCCGTGAAGTC+GGG - chr1_4:74873061-74873080 None:intergenic 55.0%
CAAGTTGTGGCCCTTCTGAC+CGG + chr1_4:74873263-74873282 Msa0185030:CDS 55.0%
! GCACCGACGGAAGCTTATGA+CGG - chr1_4:74873316-74873335 None:intergenic 55.0%
TCCCCCTCCAAGATGGGGTT+TGG - chr1_4:74871104-74871123 None:intergenic 60.0%
! CAAGGATCGGCTTCAGGCCT+CGG + chr1_4:74871339-74871358 Msa0185030:CDS 60.0%
GGCGCCAAAGGACTATCTGG+GGG - chr1_4:74871457-74871476 None:intergenic 60.0%
CGGCGCCAAAGGACTATCTG+GGG - chr1_4:74871458-74871477 None:intergenic 60.0%
GCGGCGCCAAAGGACTATCT+GGG - chr1_4:74871459-74871478 None:intergenic 60.0%
TGCGGCGCCAAAGGACTATC+TGG - chr1_4:74871460-74871479 None:intergenic 60.0%
GCATCCGAGTGCCAAGCTTC+TGG - chr1_4:74871574-74871593 None:intergenic 60.0%
TCGGATGCCGCGACTGTTAG+TGG + chr1_4:74871586-74871605 Msa0185030:CDS 60.0%
CGAGCTGGCTAACGGTCAAC+GGG - chr1_4:74872617-74872636 None:intergenic 60.0%
TGAGCCACCGCTGAGAAAGG+AGG + chr1_4:74872689-74872708 Msa0185030:CDS 60.0%
GGCTGCAGTCTTTGCTGAGC+TGG + chr1_4:74873165-74873184 Msa0185030:CDS 60.0%
CGGTCAGAAGGGCCACAACT+TGG - chr1_4:74873265-74873284 None:intergenic 60.0%
CGAGGCCTGAAGCCGATCCT+TGG - chr1_4:74871341-74871360 None:intergenic 65.0%
! TCGGCTTCAGGCCTCGGTGT+TGG + chr1_4:74871345-74871364 Msa0185030:CDS 65.0%
!! GCTTCAGGCCTCGGTGTTGG+AGG + chr1_4:74871348-74871367 Msa0185030:CDS 65.0%
GCCCAACGGCCAAACAGCTC+TGG + chr1_4:74872112-74872131 Msa0185030:CDS 65.0%
CGCTGAGCCACCGCTGAGAA+AGG + chr1_4:74872686-74872705 Msa0185030:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 74870304 74873385 74870304 ID=Msa0185030;Name=Msa0185030
chr1_4 mRNA 74870304 74873385 74870304 ID=Msa0185030-mRNA-1;Parent=Msa0185030;Name=Msa0185030-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|0.66|1|1|3|0|778
chr1_4 exon 74870304 74870312 74870304 ID=Msa0185030-mRNA-1:exon:25504;Parent=Msa0185030-mRNA-1
chr1_4 exon 74870984 74870992 74870984 ID=Msa0185030-mRNA-1:exon:25505;Parent=Msa0185030-mRNA-1
chr1_4 exon 74871067 74873385 74871067 ID=Msa0185030-mRNA-1:exon:25506;Parent=Msa0185030-mRNA-1
chr1_4 CDS 74870304 74870312 74870304 ID=Msa0185030-mRNA-1:cds;Parent=Msa0185030-mRNA-1
chr1_4 CDS 74870984 74870992 74870984 ID=Msa0185030-mRNA-1:cds;Parent=Msa0185030-mRNA-1
chr1_4 CDS 74871067 74873385 74871067 ID=Msa0185030-mRNA-1:cds;Parent=Msa0185030-mRNA-1
Gene Sequence

>Msa0185030

ATGCTGAAAGCTTCTGAAAGGCCACCGCCTGAAGATTTTCTGTTGAAAGAGACCAAACCCCATCTTGGAGGGGGAAAAATCTCTGGTGACAAACTAACCAGCACATATGACCTTGTTGAGCAGATGCAATACCTTTACGTGAGGGTTGTTAAGGCTAAGGACTTGCCTTCAAAGGATGTTACCGGAAGTTGTGATCCGTACGCTGAAGTTAAGCTCGGGAACTATAAAGGAACTACTCGGCATTTTGAGAAGAAAACCAATCCTGAATGGAATCAGGTTTTTGCTTTCTCCAAGGATCGGCTTCAGGCCTCGGTGTTGGAGGTTACTGTGAAAGATAAGGATGTTGTGAAGGACGACTTCATTGGTCGTGTGTGGTTTGATTTGAATGAGGTTCCGAAACGGGTTCCCCCAGATAGTCCTTTGGCGCCGCAATGGTATAGATTGGAGGATAGAAAGGGTGATAAGGTTAAGGGAGAGTTAATGCTTGCTGTTTGGATGGGTACACAGGCTGATGAAGCATTTCCAGAAGCTTGGCACTCGGATGCCGCGACTGTTAGTGGAACCGATGCTCTTGCAAATATTAGGTCGAAAGTTTATTTATCTCCTAAGCTTTGGTATTTGAGGGTAAATGTGATTGAGGCACAAGACTTACAGCCAACTGATAAGGGTAGATATCCGGAAGTTTTTGTGAAGGCTATTTTGGGGAATCAGGCATTGAGGACTAGAATCTCTCTTAGCAGGAGTATCAATCCATTGTGGAACGAGGATTTGATGTTCGTTGCTGCGGAACCATTTGAGGAGCCGCTGATTTTGAGTGTGGAAGACAGAGTTGCTCCGAACAAAGAAGAAGTATTGGGGAGGTGTGCAATTCCTTTGCAGTTTATGGATAGGAGACTCGACCACAAACCAGTGAATACTAGGTGGTTTAATCTTGAAAAACATATCATTGTAGAAGGGGAAAAGAAGAAAGAAATTAAATTTGCAAGTAGGATTCACATGAGGGTTTGTCTAGAAGGTGGTTATCATGTTTTGGATGAATCAACTCACTACAGCAGTGATCTTCGCCCAACGGCCAAACAGCTCTGGAAATCTGGAATTGGTGTTCTTGAAGTTGGGATATTGAGTGCTCAGGGTTTGATGCCAATGAAAAATAAAGATGGGAGGGGGACAACTGATGCTTATTGTGTAGCGAAATATGGGCAGAAGTGGATTCGAACAAGGACAATCATTGATAGCTTTATGCCGAGGTGGAATGAGCAATATACTTGGGAGGTTTTTGATCCTTGCACTGTGATTACAATTGGTGTATTTGATAATTGTCATTTGCATGGTCCTGACAAAGCTGGAGGTGCAAAAGATTCAAGGATTGGTAAGGTAAGGATTCGACTTTCGACACTCGAGACTGATCGAGTGTACACACATTCCTATCCACTCCTAGTTCTTCACCCAAGTGGTGTGAAGAAAATGGGTGAAATTCAATTGGCTGTGAGGTTCACTTGTTCTTCTTTGCTCAACATGATGCACATGTATTCACTACCTCTGTTGCCAAAGATGCACTATATACACCCGTTGACCGTTAGCCAGCTCGACAGTTTGAGGCATCAAGCTACTCAGATAGTTTCAATGAGGCTGAGTCGCGCTGAGCCACCGCTGAGAAAGGAGGTAGTTGAGTATATGCTTGATGTTGGTTCCCACATGTGGAGTATGAGAAGAAGCAAGGCTAATTTCTTCAGAATCATGGGAGTATTGAGTGGACTAATTGCTGCGGGAAAATGGTTCGATCAAATTTGCAACTGGAAAAATCCAATTACAACAGTTCTGATTCATATCTTGTTCATAATATTGGTCATGTATCCTGAACTTATCTTACCAACAGTTTTCCTTTACCTCTTCCTGATTGGAATTTGGCACTATAGATGGAGGCCAAGACACCCTCCTAACATGGACACTCGGCTCTCTCACGCCGATTCAGCTCATCCGGACGAACTTGATGAAGAGTTCGACACATTCCCGACTTCACGGCCTTCTGACATTGTTCGGATGAGATATGATCGACTTAGAAGCATAGCTGGTAGGATACAGACTGTGGTTGGCGACTTAGCTACTCAAGGAGAAAGGCTGCAGTCTTTGCTGAGCTGGCGTGATCCAAGAGCAACAGCACTGTTTGTGATTTTCTGTTTGCTTGCTGCTACTATACTGTATGTGACTCCATTCCAAGTTGTGGCCCTTCTGACCGGAATTTATGTGTTGAGACATCCGAGGTTCCGTCATAAGCTTCCGTCGGTGCCGCTTAATTTCTTCAGGAGGCTGCCTGCAAGAACTGATTGCATGCTTTGA
Protein sequence

>Msa0185030

MLKASERPPPEDFLLKETKPHLGGGKISGDKLTSTYDLVEQMQYLYVRVVKAKDLPSKDVTGSCDPYAEVKLGNYKGTTRHFEKKTNPEWNQVFAFSKDRLQASVLEVTVKDKDVVKDDFIGRVWFDLNEVPKRVPPDSPLAPQWYRLEDRKGDKVKGELMLAVWMGTQADEAFPEAWHSDAATVSGTDALANIRSKVYLSPKLWYLRVNVIEAQDLQPTDKGRYPEVFVKAILGNQALRTRISLSRSINPLWNEDLMFVAAEPFEEPLILSVEDRVAPNKEEVLGRCAIPLQFMDRRLDHKPVNTRWFNLEKHIIVEGEKKKEIKFASRIHMRVCLEGGYHVLDESTHYSSDLRPTAKQLWKSGIGVLEVGILSAQGLMPMKNKDGRGTTDAYCVAKYGQKWIRTRTIIDSFMPRWNEQYTWEVFDPCTVITIGVFDNCHLHGPDKAGGAKDSRIGKVRIRLSTLETDRVYTHSYPLLVLHPSGVKKMGEIQLAVRFTCSSLLNMMHMYSLPLLPKMHYIHPLTVSQLDSLRHQATQIVSMRLSRAEPPLRKEVVEYMLDVGSHMWSMRRSKANFFRIMGVLSGLIAAGKWFDQICNWKNPITTVLIHILFIILVMYPELILPTVFLYLFLIGIWHYRWRPRHPPNMDTRLSHADSAHPDELDEEFDTFPTSRPSDIVRMRYDRLRSIAGRIQTVVGDLATQGERLQSLLSWRDPRATALFVIFCLLAATILYVTPFQVVALLTGIYVLRHPRFRHKLPSVPLNFFRRLPARTDCML*