AlfalfaGEDB | Msa0170260

M. sativa cultivar zhongmu-4 / Msa0170260

KEGG: K12119
GO: GO:0000003 GO:0000166 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0006139 GO:0006325 GO:0006338 GO:0006725 GO:0006732 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007623 GO:0008144 GO:0008150 GO:0008152 GO:0009117 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009637 GO:0009638 GO:0009642 GO:0009645 GO:0009646 GO:0009648 GO:0009719 GO:0009725 GO:0009785 GO:0009791 GO:0009881 GO:0009882 GO:0009888 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010033 GO:0010035 GO:0010073 GO:0010075 GO:0010118 GO:0010228 GO:0010244 GO:0010617 GO:0014070 GO:0016043 GO:0016604 GO:0017076 GO:0019222 GO:0019637 GO:0019725 GO:0022414 GO:0022603 GO:0023052 GO:0030003 GO:0030522 GO:0030554 GO:0031323 GO:0031325 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033993 GO:0034641 GO:0035639 GO:0036094 GO:0038023 GO:0040008 GO:0042221 GO:0042592 GO:0042726 GO:0042752 GO:0042802 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048037 GO:0048507 GO:0048509 GO:0048511 GO:0048518 GO:0048522 GO:0048571 GO:0048573 GO:0048574 GO:0048580 GO:0048582 GO:0048583 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050660 GO:0050662 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0050896 GO:0051094 GO:0051186 GO:0051239 GO:0051240 GO:0051276 GO:0051480 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055086 GO:0060089 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071949 GO:0072387 GO:0072503 GO:0072507 GO:0097159 GO:0097367 GO:0098542 GO:0098771 GO:0104004 GO:1900618 GO:1901265 GO:1901360 GO:1901363 GO:1901371 GO:1901564 GO:1901700 GO:1902347 GO:1905421 GO:2000024 GO:2000026 GO:2000028 GO:2000241 GO:2000243 GO:2000377 GO:2000379
PFAM: PF03441.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0170260	KEH42771.1	98.039	204	4	0	1	204	436	639	1.19e-139	414

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0170260	sp\|Q96524\|CRY2_ARATH	51.000	100	43	2	1	100	440	533	7.08e-22	95.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0170260	A0A072VMD0	98.039	204	4	0	1	204	436	639	5.70e-140	414

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0170230	Msa0170260	0.888379	6.218434e-73	-8.615850e-47
Msa0170260	Msa0644140	0.803660	3.135729e-49	-8.615850e-47
Msa0170260	Msa0842620	0.800829	1.199565e-48	-8.615850e-47
Msa0170260	Msa1014990	0.806161	9.410596e-50	-8.615850e-47
Msa0170260	Msa1284680	0.807200	5.676858e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0170260	MtrunA17_Chr1g0187801	98.446	193	3	0	1	193	436	628	7.44e-136	394
Msa0170260	MtrunA17_Chr1g0167721	61.765	170	58	6	1	166	434	600	1.26e-51	175
Msa0170260	MtrunA17_Chr5g0426121	44.144	111	52	2	1	101	438	548	1.54e-24	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0170260	AT1G04400.1	51.000	100	43	2	1	100	440	533	7.20e-23	95.9
Msa0170260	AT1G04400.2	51.000	100	43	2	1	100	440	533	7.20e-23	95.9
Msa0170260	AT4G08920.1	42.727	110	53	4	1	101	443	551	3.55e-21	91.3

Find 37 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAGAAAGTGCCAGCATTTC+AGG	0.164857	1_4:+54208382	Msa0170260:CDS
GCCATATCTATATCAATTAT+TGG	0.178502	1_4:-54208188	None:intergenic
CATTTCCCACATCTTGAATA+TGG	0.213847	1_4:-54208231	None:intergenic
CTAATAGGTAGTTCATTCTT+AGG	0.252046	1_4:-54208407	None:intergenic
CCTCATCTTCAAATCTAAAA+TGG	0.261221	1_4:+54208606	Msa0170260:CDS
TCCTTAAGGAGGACAACTTT+TGG	0.310889	1_4:-54208326	None:intergenic
CTAAGAATGAACTACCTATT+AGG	0.318143	1_4:+54208408	Msa0170260:CDS
CAAGCAGCATCAAAAGCTTC+TGG	0.338602	1_4:+54208254	Msa0170260:CDS
CTGTAGAGCAAGTGTCTTCA+TGG	0.357346	1_4:-54208517	None:intergenic
TCCAAAAGTTGTCCTCCTTA+AGG	0.363395	1_4:+54208325	Msa0170260:CDS
TCCAATAATTGATATAGATA+TGG	0.381151	1_4:+54208187	Msa0170260:CDS
TCTATATCAATTATTGGATT+CGG	0.400812	1_4:-54208182	None:intergenic
CTTAGTGTGCTCAGAGCATC+AGG	0.402882	1_4:+54208137	Msa0170260:CDS
TCTTGAATATGGCTTGAGTC+AGG	0.448589	1_4:-54208220	None:intergenic
ACTATCTTTCACATGATTCA+CGG	0.453888	1_4:-54208477	None:intergenic
TTCTTAGGATCCTGAAATGC+TGG	0.457403	1_4:-54208392	None:intergenic
CTGAGTGGATCCATCATCCT+TGG	0.471071	1_4:+54208105	Msa0170260:CDS
ACTGACATGGAGCAAAGCTC+AGG	0.479208	1_4:+54208644	Msa0170260:CDS
TTGTGGAACTGAAAATGAAT+AGG	0.492993	1_4:-54208576	None:intergenic
TGAAGAGAAGAGAAAGAACA+AGG	0.511587	1_4:+54208448	Msa0170260:CDS
ATGTCAGTCTGTTCTTGCCA+TGG	0.511755	1_4:-54208629	None:intergenic
AGCTTCTGGCTCTGAAGCAA+GGG	0.519234	1_4:+54208268	Msa0170260:CDS
AAGCTTCTGGCTCTGAAGCA+AGG	0.524562	1_4:+54208267	Msa0170260:CDS
GAGCAAAGCTCAGGGAAAGA+TGG	0.532049	1_4:+54208653	Msa0170260:CDS
CTCAAGCCATATTCAAGATG+TGG	0.544153	1_4:+54208225	Msa0170260:CDS
GTGGTGCATTCCAAGGATGA+TGG	0.551180	1_4:-54208115	None:intergenic
CTTCAAATCTAAAATGGCCA+TGG	0.558284	1_4:+54208612	Msa0170260:CDS
AGAGCATCAGGAGTCGAGCT+AGG	0.561014	1_4:+54208149	Msa0170260:CDS
ACACTAAGTGGTGCATTCCA+AGG	0.571610	1_4:-54208122	None:intergenic
TCAAGCCATATTCAAGATGT+GGG	0.581508	1_4:+54208226	Msa0170260:CDS
GGATGATGGATCCACTCAGT+TGG	0.589489	1_4:-54208101	None:intergenic
GAAGATGAGGAACACTGTTG+TGG	0.596624	1_4:-54208593	None:intergenic
CTGACATGGAGCAAAGCTCA+GGG	0.616857	1_4:+54208645	Msa0170260:CDS
TTAGCTGAAATAGTAGCAGA+CGG	0.651586	1_4:-54208356	None:intergenic
GATGCTCTGAGCACACTAAG+TGG	0.654212	1_4:-54208134	None:intergenic
TGGCGAGAATGCCAACTGAG+TGG	0.660213	1_4:+54208090	None:intergenic
ATGGCAAGAACAGACTGACA+TGG	0.671542	1_4:+54208631	Msa0170260:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTATATCAATTATTGGATT+CGG	-	chr1_4:54208185-54208204	None:intergenic	20.0%
!!	TCCAATAATTGATATAGATA+TGG	+	chr1_4:54208187-54208206	Msa0170260:CDS	20.0%
!	GCCATATCTATATCAATTAT+TGG	-	chr1_4:54208191-54208210	None:intergenic	25.0%
	CTAATAGGTAGTTCATTCTT+AGG	-	chr1_4:54208410-54208429	None:intergenic	30.0%
	CTAAGAATGAACTACCTATT+AGG	+	chr1_4:54208408-54208427	Msa0170260:CDS	30.0%
!	CATTTTGTTCTCTTCCTAAT+AGG	-	chr1_4:54208425-54208444	None:intergenic	30.0%
	ACTATCTTTCACATGATTCA+CGG	-	chr1_4:54208480-54208499	None:intergenic	30.0%
	TTGTGGAACTGAAAATGAAT+AGG	-	chr1_4:54208579-54208598	None:intergenic	30.0%
!	CCATTTTAGATTTGAAGATG+AGG	-	chr1_4:54208609-54208628	None:intergenic	30.0%
	CCTCATCTTCAAATCTAAAA+TGG	+	chr1_4:54208606-54208625	Msa0170260:CDS	30.0%
	TCAAGCCATATTCAAGATGT+GGG	+	chr1_4:54208226-54208245	Msa0170260:CDS	35.0%
	CATTTCCCACATCTTGAATA+TGG	-	chr1_4:54208234-54208253	None:intergenic	35.0%
	TTAGCTGAAATAGTAGCAGA+CGG	-	chr1_4:54208359-54208378	None:intergenic	35.0%
	TGAAGAGAAGAGAAAGAACA+AGG	+	chr1_4:54208448-54208467	Msa0170260:CDS	35.0%
!	CTTCAAATCTAAAATGGCCA+TGG	+	chr1_4:54208612-54208631	Msa0170260:CDS	35.0%
	TCTTGAATATGGCTTGAGTC+AGG	-	chr1_4:54208223-54208242	None:intergenic	40.0%
	CTCAAGCCATATTCAAGATG+TGG	+	chr1_4:54208225-54208244	Msa0170260:CDS	40.0%
	TCCAAAAGTTGTCCTCCTTA+AGG	+	chr1_4:54208325-54208344	Msa0170260:CDS	40.0%
!	TCCTTAAGGAGGACAACTTT+TGG	-	chr1_4:54208329-54208348	None:intergenic	40.0%
	TTCTTAGGATCCTGAAATGC+TGG	-	chr1_4:54208395-54208414	None:intergenic	40.0%
	ACACTAAGTGGTGCATTCCA+AGG	-	chr1_4:54208125-54208144	None:intergenic	45.0%
	CAAGCAGCATCAAAAGCTTC+TGG	+	chr1_4:54208254-54208273	Msa0170260:CDS	45.0%
!!	ACGGAGCTTTTTCCTTAAGG+AGG	-	chr1_4:54208340-54208359	None:intergenic	45.0%
!!	CAGACGGAGCTTTTTCCTTA+AGG	-	chr1_4:54208343-54208362	None:intergenic	45.0%
!!	TCAGAAAGTGCCAGCATTTC+AGG	+	chr1_4:54208382-54208401	Msa0170260:CDS	45.0%
	CTGTAGAGCAAGTGTCTTCA+TGG	-	chr1_4:54208520-54208539	None:intergenic	45.0%
	GAAGATGAGGAACACTGTTG+TGG	-	chr1_4:54208596-54208615	None:intergenic	45.0%
	ATGTCAGTCTGTTCTTGCCA+TGG	-	chr1_4:54208632-54208651	None:intergenic	45.0%
	ATGGCAAGAACAGACTGACA+TGG	+	chr1_4:54208631-54208650	Msa0170260:CDS	45.0%
!!	GGATGATGGATCCACTCAGT+TGG	-	chr1_4:54208104-54208123	None:intergenic	50.0%
	CTGAGTGGATCCATCATCCT+TGG	+	chr1_4:54208105-54208124	Msa0170260:CDS	50.0%
	GTGGTGCATTCCAAGGATGA+TGG	-	chr1_4:54208118-54208137	None:intergenic	50.0%
!	GATGCTCTGAGCACACTAAG+TGG	-	chr1_4:54208137-54208156	None:intergenic	50.0%
!	CTTAGTGTGCTCAGAGCATC+AGG	+	chr1_4:54208137-54208156	Msa0170260:CDS	50.0%
!!	AAGCTTCTGGCTCTGAAGCA+AGG	+	chr1_4:54208267-54208286	Msa0170260:CDS	50.0%
!!	AGCTTCTGGCTCTGAAGCAA+GGG	+	chr1_4:54208268-54208287	Msa0170260:CDS	50.0%
	ACTGACATGGAGCAAAGCTC+AGG	+	chr1_4:54208644-54208663	Msa0170260:CDS	50.0%
	CTGACATGGAGCAAAGCTCA+GGG	+	chr1_4:54208645-54208664	Msa0170260:CDS	50.0%
	GAGCAAAGCTCAGGGAAAGA+TGG	+	chr1_4:54208653-54208672	Msa0170260:CDS	50.0%
!	AGAGCATCAGGAGTCGAGCT+AGG	+	chr1_4:54208149-54208168	Msa0170260:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	54208098	54208712	54208098	ID=Msa0170260;Name=Msa0170260
chr1_4	mRNA	54208098	54208712	54208098	ID=Msa0170260-mRNA-1;Parent=Msa0170260;Name=Msa0170260-mRNA-1;_AED=0.51;_eAED=0.51;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|204
chr1_4	exon	54208098	54208712	54208098	ID=Msa0170260-mRNA-1:exon:16192;Parent=Msa0170260-mRNA-1
chr1_4	CDS	54208098	54208712	54208098	ID=Msa0170260-mRNA-1:cds;Parent=Msa0170260-mRNA-1

Gene Sequence

>Msa0170260

ATGCCAACTGAGTGGATCCATCATCCTTGGAATGCACCACTTAGTGTGCTCAGAGCATCAGGAGTCGAGCTAGGTCAAAACTATCCGAATCCAATAATTGATATAGATATGGCTAGAGAAAACCTGACTCAAGCCATATTCAAGATGTGGGAAATGCAAGCAGCATCAAAAGCTTCTGGCTCTGAAGCAAGGGATGAAGTTGTTGCTGAAAGTGAATATCTAGACATTCCAAAAGTTGTCCTCCTTAAGGAAAAAGCTCCGTCTGCTACTATTTCAGCTAATGATCAGAAAGTGCCAGCATTTCAGGATCCTAAGAATGAACTACCTATTAGGAAGAGAACAAAATGTTTTGAAGAGAAGAGAAAGAACAAGGAAAATTCCGTGAATCATGTGAAAGATAGTAATGTTTCAAGCTCTGACCATGAAGACACTTGCTCTACAGCTGACTCTTCGCGTAAGAAGCAGTGTAGTACAAGCACCTATTCATTTTCAGTTCCACAACAGTGTTCCTCATCTTCAAATCTAAAATGGCCATGGCAAGAACAGACTGACATGGAGCAAAGCTCAGGGAAAGATGGTAAGCGTATAAGAGTGAAGACTCATTTTAGTGCCTGA

Protein sequence

>Msa0170260

MPTEWIHHPWNAPLSVLRASGVELGQNYPNPIIDIDMARENLTQAIFKMWEMQAASKASGSEARDEVVAESEYLDIPKVVLLKEKAPSATISANDQKVPAFQDPKNELPIRKRTKCFEEKRKNKENSVNHVKDSNVSSSDHEDTCSTADSSRKKQCSTSTYSFSVPQQCSSSSNLKWPWQEQTDMEQSSGKDGKRIRVKTHFSA*