AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0842620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842620 MtrunA17_Chr6g0478601 100.000 26 0 0 9 34 292 317 1.80e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842620 AT3G17850.1 100.000 25 0 0 10 34 1064 1088 1.65e-11 57.8

Find 40 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATATTATTGATATATTTA+TGG 0.125983 5_4:+19764628 Msa0842620:three_prime_UTR
GTCCCATATCTTGTTATATT+CGG 0.209404 5_4:+19764177 Msa0842620:CDS
TTCCCGAATATAACAAGATA+TGG 0.277070 5_4:-19764180 None:intergenic
CGGGAATGCCTTTGACATCT+TGG 0.344845 5_4:+19764197 Msa0842620:CDS
TCTCTCTAGTTGTACCTCTA+TGG 0.346394 5_4:-19764356 None:intergenic
AGGTACAACTAGAGAGATAC+TGG 0.350120 5_4:+19764362 Msa0842620:three_prime_UTR
TAAAGTAATTATCATAATAT+AGG 0.371592 5_4:-19764549 None:intergenic
TCCCATATCTTGTTATATTC+GGG 0.374766 5_4:+19764178 Msa0842620:CDS
AAGCTGAAGCAGCCCTTTGT+TGG 0.378313 5_4:-19764439 None:intergenic
CGTTCCCTTCCTCCGTTCTC+AGG 0.381241 5_4:-19763463 None:intergenic
ATCTCAGGAGCTAAGTAATC+AGG 0.429237 5_4:-19763514 None:intergenic
CTTCAAGTGTTTCTTAAGCT+TGG 0.432244 5_4:+19764261 Msa0842620:three_prime_UTR
TGTTGGTTGGCTCAAGTACA+AGG 0.448590 5_4:-19764422 None:intergenic
TGAAGCAGCCCTTTGTTGGT+TGG 0.453365 5_4:-19764435 None:intergenic
CAAGCTTAAGAAACACTTGA+AGG 0.456090 5_4:-19764260 None:intergenic
CCAGTTCCTAAAAGTATCTC+AGG 0.463458 5_4:-19763529 None:intergenic
CTTCAGCTTCTGAAATATTG+AGG 0.464148 5_4:+19764453 Msa0842620:three_prime_UTR
AATTACTCAAGAAAAGTTGA+AGG 0.472676 5_4:+19764481 Msa0842620:three_prime_UTR
AGGAAGAAGCGTTCCGCTGT+TGG 0.509264 5_4:+19763487 Msa0842620:CDS
TACTTGAGCCAACCAACAAA+GGG 0.523572 5_4:+19764427 Msa0842620:three_prime_UTR
TCCCGAATATAACAAGATAT+GGG 0.527873 5_4:-19764179 None:intergenic
CTTTGTTGACATACTAATGT+TGG 0.530532 5_4:+19764155 Msa0842620:CDS
TAGCTCCTGAGAACGGAGGA+AGG 0.550507 5_4:+19763458 Msa0842620:intron
AGAAACACTTGAAGGATACA+TGG 0.567548 5_4:-19764252 None:intergenic
GTACTTGAGCCAACCAACAA+AGG 0.575699 5_4:+19764426 Msa0842620:three_prime_UTR
TTGTACAATCTGCCTCAGCT+TGG 0.578994 5_4:-19764293 None:intergenic
GAATGCCTTTGACATCTTGG+TGG 0.583809 5_4:+19764200 Msa0842620:CDS
CTGAGAACGGAGGAAGGGAA+CGG 0.588381 5_4:+19763464 Msa0842620:CDS
GATTACTTAGCTCCTGAGAA+CGG 0.591680 5_4:+19763451 Msa0842620:intron
AGCTCCTGAGAACGGAGGAA+GGG 0.604860 5_4:+19763459 Msa0842620:intron
TCACAGTTAAAAGATATGTG+TGG 0.617575 5_4:+19763114 None:intergenic
ATGAAGGCATACTTCCATAG+AGG 0.619428 5_4:+19764342 Msa0842620:three_prime_UTR
GTTGGTTGGCTCAAGTACAA+GGG 0.633662 5_4:-19764421 None:intergenic
GATCACCACCAAGATGTCAA+AGG 0.647393 5_4:-19764205 None:intergenic
CAACATTAGTATGTCAACAA+AGG 0.660405 5_4:-19764154 None:intergenic
AGAACGGAGGAAGGGAACGG+AGG 0.664935 5_4:+19763467 Msa0842620:CDS
GGTACAACTAGAGAGATACT+GGG 0.665492 5_4:+19764363 Msa0842620:three_prime_UTR
GTAATCAGGTGTGCCAACAG+CGG 0.675334 5_4:-19763500 None:intergenic
TGGCAGTTATCTCCAAGCTG+AGG 0.688754 5_4:+19764281 Msa0842620:three_prime_UTR
TACTTAGCTCCTGAGAACGG+AGG 0.696955 5_4:+19763454 Msa0842620:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTATATTTTTATTCTTT+TGG + chr5_4:19763841-19763860 Msa0842620:intron 10.0%
!! TAAAGTAATTATCATAATAT+AGG - chr5_4:19764552-19764571 None:intergenic 10.0%
!! GAATATTATTGATATATTTA+TGG + chr5_4:19764628-19764647 Msa0842620:three_prime_UTR 10.0%
!!! TGTTTATATTTTTATTCTTT+TGG + chr5_4:19763841-19763860 Msa0842620:intron 10.0%
!! TAAAGTAATTATCATAATAT+AGG - chr5_4:19764552-19764571 None:intergenic 10.0%
!! GAATATTATTGATATATTTA+TGG + chr5_4:19764628-19764647 Msa0842620:three_prime_UTR 10.0%
!!! GTTAGTTTTTTTTTTTTTGA+AGG + chr5_4:19764576-19764595 Msa0842620:three_prime_UTR 15.0%
!!! GTTAGTTTTTTTTTTTTTGA+AGG + chr5_4:19764576-19764595 Msa0842620:three_prime_UTR 15.0%
!! TTCACCTTAAACATATAAAT+GGG - chr5_4:19763571-19763590 None:intergenic 20.0%
!!! ATTTGAACAAATATTTTTGC+AGG - chr5_4:19763711-19763730 None:intergenic 20.0%
!!! AACTACCTATTTTTTGTAAT+TGG + chr5_4:19764071-19764090 Msa0842620:intron 20.0%
!! AAGAACCAATTACAAAAAAT+AGG - chr5_4:19764079-19764098 None:intergenic 20.0%
!! TTCACCTTAAACATATAAAT+GGG - chr5_4:19763571-19763590 None:intergenic 20.0%
!!! ATTTGAACAAATATTTTTGC+AGG - chr5_4:19763711-19763730 None:intergenic 20.0%
!!! AACTACCTATTTTTTGTAAT+TGG + chr5_4:19764071-19764090 Msa0842620:intron 20.0%
!! AAGAACCAATTACAAAAAAT+AGG - chr5_4:19764079-19764098 None:intergenic 20.0%
! AAAGATTTACATACAACTTC+AGG - chr5_4:19763217-19763236 None:intergenic 25.0%
! ATTATTAAGATGCAATTGCA+AGG + chr5_4:19763239-19763258 Msa0842620:intron 25.0%
!! TTGGTTCAATAGTAAGATTT+TGG + chr5_4:19763304-19763323 Msa0842620:intron 25.0%
! TAGTCCCATTTATATGTTTA+AGG + chr5_4:19763564-19763583 Msa0842620:intron 25.0%
! GTTCACCTTAAACATATAAA+TGG - chr5_4:19763572-19763591 None:intergenic 25.0%
! GGCTACTATAATAACAAAAA+GGG - chr5_4:19763611-19763630 None:intergenic 25.0%
! AGGCTACTATAATAACAAAA+AGG - chr5_4:19763612-19763631 None:intergenic 25.0%
!!! ATTTTATTTGAGTTGCTTGT+TGG + chr5_4:19763770-19763789 Msa0842620:intron 25.0%
! ACACAAAATTACATAATCTG+TGG - chr5_4:19763885-19763904 None:intergenic 25.0%
! AATTACTCAAGAAAAGTTGA+AGG + chr5_4:19764481-19764500 Msa0842620:three_prime_UTR 25.0%
! AAAGATTTACATACAACTTC+AGG - chr5_4:19763217-19763236 None:intergenic 25.0%
! ATTATTAAGATGCAATTGCA+AGG + chr5_4:19763239-19763258 Msa0842620:intron 25.0%
!! TTGGTTCAATAGTAAGATTT+TGG + chr5_4:19763304-19763323 Msa0842620:intron 25.0%
! TAGTCCCATTTATATGTTTA+AGG + chr5_4:19763564-19763583 Msa0842620:intron 25.0%
! GTTCACCTTAAACATATAAA+TGG - chr5_4:19763572-19763591 None:intergenic 25.0%
! GGCTACTATAATAACAAAAA+GGG - chr5_4:19763611-19763630 None:intergenic 25.0%
! AGGCTACTATAATAACAAAA+AGG - chr5_4:19763612-19763631 None:intergenic 25.0%
!!! ATTTTATTTGAGTTGCTTGT+TGG + chr5_4:19763770-19763789 Msa0842620:intron 25.0%
! ACACAAAATTACATAATCTG+TGG - chr5_4:19763885-19763904 None:intergenic 25.0%
! AATTACTCAAGAAAAGTTGA+AGG + chr5_4:19764481-19764500 Msa0842620:three_prime_UTR 25.0%
! ATGTGTCAAACATTTTTGCA+AGG + chr5_4:19763335-19763354 Msa0842620:intron 30.0%
AATCAACCGAAAAAAACACT+CGG - chr5_4:19763435-19763454 None:intergenic 30.0%
! AAAGTTAAACCTGAGTTGAA+GGG - chr5_4:19763803-19763822 None:intergenic 30.0%
TTCTTAATCGTAAGATACCT+TGG + chr5_4:19763927-19763946 Msa0842620:intron 30.0%
AAGCACATGATCTTATTGAT+CGG + chr5_4:19763978-19763997 Msa0842620:intron 30.0%
CTGTTCTTGTCATATGTATT+AGG + chr5_4:19764033-19764052 Msa0842620:intron 30.0%
CAACATTAGTATGTCAACAA+AGG - chr5_4:19764157-19764176 None:intergenic 30.0%
CTTTGTTGACATACTAATGT+TGG + chr5_4:19764155-19764174 Msa0842620:CDS 30.0%
! GTCCCATATCTTGTTATATT+CGG + chr5_4:19764177-19764196 Msa0842620:CDS 30.0%
! TCCCATATCTTGTTATATTC+GGG + chr5_4:19764178-19764197 Msa0842620:CDS 30.0%
TCCCGAATATAACAAGATAT+GGG - chr5_4:19764182-19764201 None:intergenic 30.0%
TTCCCGAATATAACAAGATA+TGG - chr5_4:19764183-19764202 None:intergenic 30.0%
! TCTTGACAAAAAGAGTATGA+AGG + chr5_4:19764326-19764345 Msa0842620:three_prime_UTR 30.0%
! ATGTGTCAAACATTTTTGCA+AGG + chr5_4:19763335-19763354 Msa0842620:intron 30.0%
AATCAACCGAAAAAAACACT+CGG - chr5_4:19763435-19763454 None:intergenic 30.0%
! AAAGTTAAACCTGAGTTGAA+GGG - chr5_4:19763803-19763822 None:intergenic 30.0%
TTCTTAATCGTAAGATACCT+TGG + chr5_4:19763927-19763946 Msa0842620:intron 30.0%
AAGCACATGATCTTATTGAT+CGG + chr5_4:19763978-19763997 Msa0842620:intron 30.0%
CTGTTCTTGTCATATGTATT+AGG + chr5_4:19764033-19764052 Msa0842620:intron 30.0%
CAACATTAGTATGTCAACAA+AGG - chr5_4:19764157-19764176 None:intergenic 30.0%
CTTTGTTGACATACTAATGT+TGG + chr5_4:19764155-19764174 Msa0842620:CDS 30.0%
! GTCCCATATCTTGTTATATT+CGG + chr5_4:19764177-19764196 Msa0842620:CDS 30.0%
! TCCCATATCTTGTTATATTC+GGG + chr5_4:19764178-19764197 Msa0842620:CDS 30.0%
TCCCGAATATAACAAGATAT+GGG - chr5_4:19764182-19764201 None:intergenic 30.0%
TTCCCGAATATAACAAGATA+TGG - chr5_4:19764183-19764202 None:intergenic 30.0%
! TCTTGACAAAAAGAGTATGA+AGG + chr5_4:19764326-19764345 Msa0842620:three_prime_UTR 30.0%
AGGAACTAATCATAGTTCTC+AGG + chr5_4:19763172-19763191 Msa0842620:intron 35.0%
GGAACTAATCATAGTTCTCA+GGG + chr5_4:19763173-19763192 Msa0842620:intron 35.0%
!!! TAACAGCCGAGTGTTTTTTT+CGG + chr5_4:19763426-19763445 Msa0842620:intron 35.0%
! TTAGCTCCTGAGATACTTTT+AGG + chr5_4:19763523-19763542 Msa0842620:CDS 35.0%
!! ATACTTTTAGGAACTGGACA+TGG + chr5_4:19763535-19763554 Msa0842620:CDS 35.0%
TGTAAAACCACTAAATCAGC+AGG - chr5_4:19763632-19763651 None:intergenic 35.0%
! AAATTTTGCAGCGTATACTG+CGG + chr5_4:19763727-19763746 Msa0842620:intron 35.0%
! CAAAGTTAAACCTGAGTTGA+AGG - chr5_4:19763804-19763823 None:intergenic 35.0%
AGAAACACTTGAAGGATACA+TGG - chr5_4:19764255-19764274 None:intergenic 35.0%
CAAGCTTAAGAAACACTTGA+AGG - chr5_4:19764263-19764282 None:intergenic 35.0%
CTTCAAGTGTTTCTTAAGCT+TGG + chr5_4:19764261-19764280 Msa0842620:three_prime_UTR 35.0%
!! CTTCAGCTTCTGAAATATTG+AGG + chr5_4:19764453-19764472 Msa0842620:three_prime_UTR 35.0%
!!! TGCAAGAATTTTGAGTTACG+AGG + chr5_4:19764507-19764526 Msa0842620:three_prime_UTR 35.0%
!!! GCAAGAATTTTGAGTTACGA+GGG + chr5_4:19764508-19764527 Msa0842620:three_prime_UTR 35.0%
AGGAACTAATCATAGTTCTC+AGG + chr5_4:19763172-19763191 Msa0842620:intron 35.0%
GGAACTAATCATAGTTCTCA+GGG + chr5_4:19763173-19763192 Msa0842620:intron 35.0%
!!! TAACAGCCGAGTGTTTTTTT+CGG + chr5_4:19763426-19763445 Msa0842620:intron 35.0%
! TTAGCTCCTGAGATACTTTT+AGG + chr5_4:19763523-19763542 Msa0842620:CDS 35.0%
!! ATACTTTTAGGAACTGGACA+TGG + chr5_4:19763535-19763554 Msa0842620:CDS 35.0%
TGTAAAACCACTAAATCAGC+AGG - chr5_4:19763632-19763651 None:intergenic 35.0%
! AAATTTTGCAGCGTATACTG+CGG + chr5_4:19763727-19763746 Msa0842620:intron 35.0%
! CAAAGTTAAACCTGAGTTGA+AGG - chr5_4:19763804-19763823 None:intergenic 35.0%
AGAAACACTTGAAGGATACA+TGG - chr5_4:19764255-19764274 None:intergenic 35.0%
CAAGCTTAAGAAACACTTGA+AGG - chr5_4:19764263-19764282 None:intergenic 35.0%
CTTCAAGTGTTTCTTAAGCT+TGG + chr5_4:19764261-19764280 Msa0842620:three_prime_UTR 35.0%
!! CTTCAGCTTCTGAAATATTG+AGG + chr5_4:19764453-19764472 Msa0842620:three_prime_UTR 35.0%
!!! TGCAAGAATTTTGAGTTACG+AGG + chr5_4:19764507-19764526 Msa0842620:three_prime_UTR 35.0%
!!! GCAAGAATTTTGAGTTACGA+GGG + chr5_4:19764508-19764527 Msa0842620:three_prime_UTR 35.0%
CAACACAGATGTGATTGAGA+AGG + chr5_4:19763152-19763171 Msa0842620:intron 40.0%
ACCACACAGAAACATGTCAT+TGG + chr5_4:19763285-19763304 Msa0842620:intron 40.0%
! ACCAATGACATGTTTCTGTG+TGG - chr5_4:19763289-19763308 None:intergenic 40.0%
ATTTAGCTACTCCACTGTCA+TGG - chr5_4:19763364-19763383 None:intergenic 40.0%
CTAAATCTCAGCATGGAGAT+TGG + chr5_4:19763378-19763397 Msa0842620:intron 40.0%
!! ATTGGCTCTGCATTTGGTTT+CGG + chr5_4:19763396-19763415 Msa0842620:intron 40.0%
! TCTGCATTTGGTTTCGGTTT+GGG + chr5_4:19763402-19763421 Msa0842620:intron 40.0%
GATTACTTAGCTCCTGAGAA+CGG + chr5_4:19763451-19763470 Msa0842620:intron 40.0%
ATCTCAGGAGCTAAGTAATC+AGG - chr5_4:19763517-19763536 None:intergenic 40.0%
!! CCAGTTCCTAAAAGTATCTC+AGG - chr5_4:19763532-19763551 None:intergenic 40.0%
!! CCTGAGATACTTTTAGGAAC+TGG + chr5_4:19763529-19763548 Msa0842620:CDS 40.0%
!! ATAGTAGCCTGCTGATTTAG+TGG + chr5_4:19763622-19763641 Msa0842620:intron 40.0%
TGCTTCAAGACTCATTTCGT+CGG - chr5_4:19763963-19763982 None:intergenic 40.0%
! GCTGTTTGTTAGTGCAGATA+TGG + chr5_4:19764131-19764150 Msa0842620:intron 40.0%
! ATGAAGGCATACTTCCATAG+AGG + chr5_4:19764342-19764361 Msa0842620:three_prime_UTR 40.0%
TCTCTCTAGTTGTACCTCTA+TGG - chr5_4:19764359-19764378 None:intergenic 40.0%
AGGTACAACTAGAGAGATAC+TGG + chr5_4:19764362-19764381 Msa0842620:three_prime_UTR 40.0%
GGTACAACTAGAGAGATACT+GGG + chr5_4:19764363-19764382 Msa0842620:three_prime_UTR 40.0%
TACTTGAGCCAACCAACAAA+GGG + chr5_4:19764427-19764446 Msa0842620:three_prime_UTR 40.0%
CAACACAGATGTGATTGAGA+AGG + chr5_4:19763152-19763171 Msa0842620:intron 40.0%
ACCACACAGAAACATGTCAT+TGG + chr5_4:19763285-19763304 Msa0842620:intron 40.0%
! ACCAATGACATGTTTCTGTG+TGG - chr5_4:19763289-19763308 None:intergenic 40.0%
ATTTAGCTACTCCACTGTCA+TGG - chr5_4:19763364-19763383 None:intergenic 40.0%
CTAAATCTCAGCATGGAGAT+TGG + chr5_4:19763378-19763397 Msa0842620:intron 40.0%
!! ATTGGCTCTGCATTTGGTTT+CGG + chr5_4:19763396-19763415 Msa0842620:intron 40.0%
! TCTGCATTTGGTTTCGGTTT+GGG + chr5_4:19763402-19763421 Msa0842620:intron 40.0%
GATTACTTAGCTCCTGAGAA+CGG + chr5_4:19763451-19763470 Msa0842620:intron 40.0%
ATCTCAGGAGCTAAGTAATC+AGG - chr5_4:19763517-19763536 None:intergenic 40.0%
!! CCAGTTCCTAAAAGTATCTC+AGG - chr5_4:19763532-19763551 None:intergenic 40.0%
!! CCTGAGATACTTTTAGGAAC+TGG + chr5_4:19763529-19763548 Msa0842620:CDS 40.0%
!! ATAGTAGCCTGCTGATTTAG+TGG + chr5_4:19763622-19763641 Msa0842620:intron 40.0%
TGCTTCAAGACTCATTTCGT+CGG - chr5_4:19763963-19763982 None:intergenic 40.0%
! GCTGTTTGTTAGTGCAGATA+TGG + chr5_4:19764131-19764150 Msa0842620:intron 40.0%
! ATGAAGGCATACTTCCATAG+AGG + chr5_4:19764342-19764361 Msa0842620:three_prime_UTR 40.0%
TCTCTCTAGTTGTACCTCTA+TGG - chr5_4:19764359-19764378 None:intergenic 40.0%
AGGTACAACTAGAGAGATAC+TGG + chr5_4:19764362-19764381 Msa0842620:three_prime_UTR 40.0%
GGTACAACTAGAGAGATACT+GGG + chr5_4:19764363-19764382 Msa0842620:three_prime_UTR 40.0%
TACTTGAGCCAACCAACAAA+GGG + chr5_4:19764427-19764446 Msa0842620:three_prime_UTR 40.0%
GGAGTAGCTAAATCTCAGCA+TGG + chr5_4:19763371-19763390 Msa0842620:intron 45.0%
!! ATGGAGATTGGCTCTGCATT+TGG + chr5_4:19763390-19763409 Msa0842620:intron 45.0%
! CTCTGCATTTGGTTTCGGTT+TGG + chr5_4:19763401-19763420 Msa0842620:intron 45.0%
! GTTAAACCTGAGTTGAAGGG+TGG - chr5_4:19763800-19763819 None:intergenic 45.0%
GCTTCAAGACTCATTTCGTC+GGG - chr5_4:19763962-19763981 None:intergenic 45.0%
GAATGCCTTTGACATCTTGG+TGG + chr5_4:19764200-19764219 Msa0842620:CDS 45.0%
GATCACCACCAAGATGTCAA+AGG - chr5_4:19764208-19764227 None:intergenic 45.0%
TTGTACAATCTGCCTCAGCT+TGG - chr5_4:19764296-19764315 None:intergenic 45.0%
! GTTGGTTGGCTCAAGTACAA+GGG - chr5_4:19764424-19764443 None:intergenic 45.0%
! TGTTGGTTGGCTCAAGTACA+AGG - chr5_4:19764425-19764444 None:intergenic 45.0%
GTACTTGAGCCAACCAACAA+AGG + chr5_4:19764426-19764445 Msa0842620:three_prime_UTR 45.0%
GGAGTAGCTAAATCTCAGCA+TGG + chr5_4:19763371-19763390 Msa0842620:intron 45.0%
!! ATGGAGATTGGCTCTGCATT+TGG + chr5_4:19763390-19763409 Msa0842620:intron 45.0%
! CTCTGCATTTGGTTTCGGTT+TGG + chr5_4:19763401-19763420 Msa0842620:intron 45.0%
! GTTAAACCTGAGTTGAAGGG+TGG - chr5_4:19763800-19763819 None:intergenic 45.0%
GCTTCAAGACTCATTTCGTC+GGG - chr5_4:19763962-19763981 None:intergenic 45.0%
GAATGCCTTTGACATCTTGG+TGG + chr5_4:19764200-19764219 Msa0842620:CDS 45.0%
GATCACCACCAAGATGTCAA+AGG - chr5_4:19764208-19764227 None:intergenic 45.0%
TTGTACAATCTGCCTCAGCT+TGG - chr5_4:19764296-19764315 None:intergenic 45.0%
! GTTGGTTGGCTCAAGTACAA+GGG - chr5_4:19764424-19764443 None:intergenic 45.0%
! TGTTGGTTGGCTCAAGTACA+AGG - chr5_4:19764425-19764444 None:intergenic 45.0%
GTACTTGAGCCAACCAACAA+AGG + chr5_4:19764426-19764445 Msa0842620:three_prime_UTR 45.0%
TTGCAAGGCTACCATGACAG+TGG + chr5_4:19763350-19763369 Msa0842620:intron 50.0%
TACTTAGCTCCTGAGAACGG+AGG + chr5_4:19763454-19763473 Msa0842620:intron 50.0%
GTAATCAGGTGTGCCAACAG+CGG - chr5_4:19763503-19763522 None:intergenic 50.0%
TTGCAGCGTATACTGCGGAT+TGG + chr5_4:19763732-19763751 Msa0842620:intron 50.0%
GGTATTCCACCCTTCAACTC+AGG + chr5_4:19763791-19763810 Msa0842620:intron 50.0%
CTCATTTCGTCGGGAACTTC+AGG - chr5_4:19763953-19763972 None:intergenic 50.0%
CGGGAATGCCTTTGACATCT+TGG + chr5_4:19764197-19764216 Msa0842620:CDS 50.0%
TGGCAGTTATCTCCAAGCTG+AGG + chr5_4:19764281-19764300 Msa0842620:three_prime_UTR 50.0%
TGAAGCAGCCCTTTGTTGGT+TGG - chr5_4:19764438-19764457 None:intergenic 50.0%
AAGCTGAAGCAGCCCTTTGT+TGG - chr5_4:19764442-19764461 None:intergenic 50.0%
TTGCAAGGCTACCATGACAG+TGG + chr5_4:19763350-19763369 Msa0842620:intron 50.0%
TACTTAGCTCCTGAGAACGG+AGG + chr5_4:19763454-19763473 Msa0842620:intron 50.0%
GTAATCAGGTGTGCCAACAG+CGG - chr5_4:19763503-19763522 None:intergenic 50.0%
TTGCAGCGTATACTGCGGAT+TGG + chr5_4:19763732-19763751 Msa0842620:intron 50.0%
GGTATTCCACCCTTCAACTC+AGG + chr5_4:19763791-19763810 Msa0842620:intron 50.0%
CTCATTTCGTCGGGAACTTC+AGG - chr5_4:19763953-19763972 None:intergenic 50.0%
CGGGAATGCCTTTGACATCT+TGG + chr5_4:19764197-19764216 Msa0842620:CDS 50.0%
TGGCAGTTATCTCCAAGCTG+AGG + chr5_4:19764281-19764300 Msa0842620:three_prime_UTR 50.0%
TGAAGCAGCCCTTTGTTGGT+TGG - chr5_4:19764438-19764457 None:intergenic 50.0%
AAGCTGAAGCAGCCCTTTGT+TGG - chr5_4:19764442-19764461 None:intergenic 50.0%
TAGCTCCTGAGAACGGAGGA+AGG + chr5_4:19763458-19763477 Msa0842620:intron 55.0%
AGCTCCTGAGAACGGAGGAA+GGG + chr5_4:19763459-19763478 Msa0842620:intron 55.0%
CTGAGAACGGAGGAAGGGAA+CGG + chr5_4:19763464-19763483 Msa0842620:CDS 55.0%
!! AGGAAGAAGCGTTCCGCTGT+TGG + chr5_4:19763487-19763506 Msa0842620:CDS 55.0%
!! CAGCGTATACTGCGGATTGG+TGG + chr5_4:19763735-19763754 Msa0842620:intron 55.0%
!! ACTGCGGATTGGTGGTCTGT+TGG + chr5_4:19763743-19763762 Msa0842620:intron 55.0%
!! CTGCGGATTGGTGGTCTGTT+GGG + chr5_4:19763744-19763763 Msa0842620:intron 55.0%
!! TGCGGATTGGTGGTCTGTTG+GGG + chr5_4:19763745-19763764 Msa0842620:intron 55.0%
TAGCTCCTGAGAACGGAGGA+AGG + chr5_4:19763458-19763477 Msa0842620:intron 55.0%
AGCTCCTGAGAACGGAGGAA+GGG + chr5_4:19763459-19763478 Msa0842620:intron 55.0%
CTGAGAACGGAGGAAGGGAA+CGG + chr5_4:19763464-19763483 Msa0842620:CDS 55.0%
!! AGGAAGAAGCGTTCCGCTGT+TGG + chr5_4:19763487-19763506 Msa0842620:CDS 55.0%
!! CAGCGTATACTGCGGATTGG+TGG + chr5_4:19763735-19763754 Msa0842620:intron 55.0%
!! ACTGCGGATTGGTGGTCTGT+TGG + chr5_4:19763743-19763762 Msa0842620:intron 55.0%
!! CTGCGGATTGGTGGTCTGTT+GGG + chr5_4:19763744-19763763 Msa0842620:intron 55.0%
!! TGCGGATTGGTGGTCTGTTG+GGG + chr5_4:19763745-19763764 Msa0842620:intron 55.0%
CGTTCCCTTCCTCCGTTCTC+AGG - chr5_4:19763466-19763485 None:intergenic 60.0%
AGAACGGAGGAAGGGAACGG+AGG + chr5_4:19763467-19763486 Msa0842620:CDS 60.0%
TCGTCGGGAACTTCAGGCCA+AGG - chr5_4:19763947-19763966 None:intergenic 60.0%
CGTTCCCTTCCTCCGTTCTC+AGG - chr5_4:19763466-19763485 None:intergenic 60.0%
AGAACGGAGGAAGGGAACGG+AGG + chr5_4:19763467-19763486 Msa0842620:CDS 60.0%
TCGTCGGGAACTTCAGGCCA+AGG - chr5_4:19763947-19763966 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 19763129 19764669 19763129 ID=Msa0842620;Name=Msa0842620
chr5_4 mRNA 19763129 19764669 19763129 ID=Msa0842620-mRNA-1;Parent=Msa0842620;Name=Msa0842620-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0.33|0.66|1|1|3|421|67
chr5_4 exon 19763129 19763135 19763129 ID=Msa0842620-mRNA-1:exon:8214;Parent=Msa0842620-mRNA-1
chr5_4 exon 19763461 19763556 19763461 ID=Msa0842620-mRNA-1:exon:8215;Parent=Msa0842620-mRNA-1
chr5_4 exon 19764148 19764669 19764148 ID=Msa0842620-mRNA-1:exon:8216;Parent=Msa0842620-mRNA-1
chr5_4 CDS 19763129 19763135 19763129 ID=Msa0842620-mRNA-1:cds;Parent=Msa0842620-mRNA-1
chr5_4 CDS 19763461 19763556 19763461 ID=Msa0842620-mRNA-1:cds;Parent=Msa0842620-mRNA-1
chr5_4 CDS 19764148 19764248 19764148 ID=Msa0842620-mRNA-1:cds;Parent=Msa0842620-mRNA-1
chr5_4 three_prime_UTR 19764249 19764669 19764249 ID=Msa0842620-mRNA-1:three_prime_utr;Parent=Msa0842620-mRNA-1
Gene Sequence

>Msa0842620

ATGTGTGCTCCTGAGAACGGAGGAAGGGAACGGAGGAAGAAGCGTTCCGCTGTTGGCACACCTGATTACTTAGCTCCTGAGATACTTTTAGGAACTGGACATGATATGGCCTTTGTTGACATACTAATGTTGGTCCCATATCTTGTTATATTCGGGAATGCCTTTGACATCTTGGTGGTGATCGCTTTTTGCTTGACACGCTAA
Protein sequence

>Msa0842620

MCAPENGGRERRKKRSAVGTPDYLAPEILLGTGHDMAFVDILMLVPYLVIFGNAFDILVVIAFCLTR*