AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0172940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172940 MtrunA17_Chr1g0192231 97.778 90 0 1 1 88 1 90 1.71e-59 176
Msa0172940 MtrunA17_Chr3g0114431 46.575 73 38 1 8 79 1 73 1.57e-18 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172940 AT3G05550.1 83.544 79 13 0 1 79 1 79 8.33e-34 112
Msa0172940 AT5G27760.1 78.205 78 17 0 1 78 1 78 1.18e-31 106
Msa0172940 AT5G27760.2 72.222 54 15 0 1 54 1 54 6.87e-27 94.0

Find 53 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGGTGAAACTTATTATT+TGG 0.155300 1_4:-58121878 Msa0172940:three_prime_UTR
TTTGGGCTTTATATTGTATT+GGG 0.313076 1_4:+58124792 None:intergenic
ATTTCCTGAGAGATTCGATT+TGG 0.327609 1_4:+58124683 None:intergenic
AAATCGAATCTCTCAGGAAA+TGG 0.335949 1_4:-58124681 Msa0172940:CDS
AAAAGGCTGCAAGGGATTCT+AGG 0.341872 1_4:-58122307 Msa0172940:CDS
CTTGTGGCTTAGCGGTATTA+CGG 0.354612 1_4:-58124524 Msa0172940:CDS
TTGTGGCTTAGCGGTATTAC+GGG 0.360744 1_4:-58124523 Msa0172940:CDS
TCAGCTAGTATAAAGAATTT+CGG 0.373467 1_4:+58122258 None:intergenic
ACATTATTGCTTGCTTGCTT+AGG 0.385386 1_4:+58124719 None:intergenic
CGGGTTCAATTGCGTACAAT+TGG 0.387745 1_4:-58124504 Msa0172940:CDS
ATTATTTATTAATTGATAGT+TGG 0.403725 1_4:-58121960 Msa0172940:three_prime_UTR
CATTGAAATATTTACTTGCC+AGG 0.410212 1_4:-58122130 Msa0172940:three_prime_UTR
ACCACTTCATTTGTAACACT+TGG 0.413631 1_4:-58121843 Msa0172940:three_prime_UTR
CGCAAGTTCTTTAACTCCAC+GGG 0.423820 1_4:-58121902 Msa0172940:three_prime_UTR
CTTACACTGGGAGCATTAGC+TGG 0.431990 1_4:-58122368 Msa0172940:CDS
TTTCCTGAGAGATTCGATTT+GGG 0.436962 1_4:+58124684 None:intergenic
TTTCTTGTTGCAAATTGCAT+TGG 0.444422 1_4:-58122098 Msa0172940:three_prime_UTR
TGATCACAAGTCTGAAGAAA+AGG 0.467242 1_4:-58122324 Msa0172940:CDS
AATTTGCTGTGGTTCATCAA+TGG 0.470506 1_4:-58122170 Msa0172940:three_prime_UTR
AATCGAATCTCTCAGGAAAT+GGG 0.471588 1_4:-58124680 Msa0172940:CDS
CAACTATTGATTTATATATG+AGG 0.481886 1_4:-58122001 Msa0172940:three_prime_UTR
AAACCCAAATCGAATCTCTC+AGG 0.502597 1_4:-58124687 Msa0172940:CDS
TTAGGTTGCTTGTGGCTTAG+CGG 0.511647 1_4:-58124532 Msa0172940:intron
TCTTTAACTCCACGGGGATG+AGG 0.522631 1_4:-58121895 Msa0172940:three_prime_UTR
GCATGCTCAAGCTCTTACAC+TGG 0.522809 1_4:-58122381 Msa0172940:CDS
GTCTGAAGAAAAGGCTGCAA+GGG 0.524443 1_4:-58122315 Msa0172940:CDS
GTTCCGCCGCGTCATCAAGC+AGG 0.530700 1_4:-58124876 Msa0172940:five_prime_UTR
CCACTTCATTTGTAACACTT+GGG 0.535710 1_4:-58121842 Msa0172940:three_prime_UTR
GTGTTAAGATTATTCACGCC+AGG 0.537077 1_4:-58124459 Msa0172940:intron
AACAAGAAATATATTCTCCC+TGG 0.539373 1_4:+58122112 None:intergenic
ATTGAAATATTTACTTGCCA+GGG 0.543373 1_4:-58122129 Msa0172940:three_prime_UTR
GTATCTGACATCTCACAGCT+TGG 0.565513 1_4:-58122230 Msa0172940:three_prime_UTR
TTGGAAATTTACCAACAGTA+CGG 0.567029 1_4:+58124641 None:intergenic
TTGCTTGCTTGCTTAGGAGA+GGG 0.573235 1_4:+58124725 None:intergenic
AGCATTAGCTGGAGCTGCAG+TGG 0.578637 1_4:-58122357 Msa0172940:CDS
CCCAAGTGTTACAAATGAAG+TGG 0.581164 1_4:+58121842 None:intergenic
CATGCTCAAGCTCTTACACT+GGG 0.582325 1_4:-58122380 Msa0172940:CDS
GAACACAAACTCCGTACTGT+TGG 0.585849 1_4:-58124652 Msa0172940:intron
AGTCTGAAGAAAAGGCTGCA+AGG 0.589757 1_4:-58122316 Msa0172940:CDS
TTTCTCGATTAGGTTGCTTG+TGG 0.590665 1_4:-58124540 Msa0172940:intron
ACGCAAGTTCTTTAACTCCA+CGG 0.590810 1_4:-58121903 Msa0172940:three_prime_UTR
TGACGCGGCGGAACTACTAT+CGG 0.597040 1_4:+58124885 None:intergenic
TCATTTGTAACACTTGGGCG+TGG 0.598299 1_4:-58121837 Msa0172940:three_prime_UTR
ATTGCTTGCTTGCTTAGGAG+AGG 0.609965 1_4:+58124724 None:intergenic
TTGGGAAATACTATTGCGTG+AGG 0.611327 1_4:+58124810 None:intergenic
TGTAAACCTGCTTGATGACG+CGG 0.617859 1_4:+58124870 None:intergenic
GGCGTGAATAATCTTAACAC+TGG 0.626108 1_4:+58124462 None:intergenic
AAGCAAGCAAGCAATAATGT+CGG 0.649556 1_4:-58124716 Msa0172940:exon
GTGGAGTTAAAGAACTTGCG+TGG 0.662404 1_4:+58121905 None:intergenic
TGGGAAATACTATTGCGTGA+GGG 0.685241 1_4:+58124811 None:intergenic
TAAGTTTCACCTCATCCCCG+TGG 0.693295 1_4:+58121886 None:intergenic
AAACCTGCTTGATGACGCGG+CGG 0.729792 1_4:+58124873 None:intergenic
GCAAGTTCTTTAACTCCACG+GGG 0.741920 1_4:-58121901 Msa0172940:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTATTATTATTATTT+TGG - chr1_4:58122566-58122585 Msa0172940:intron 0.0%
!! TAAATCATATCATAAAATAA+AGG + chr1_4:58122105-58122124 None:intergenic 10.0%
!!! TTTATTTTATGATATGATTT+AGG - chr1_4:58122104-58122123 Msa0172940:three_prime_UTR 10.0%
!!! ATTGAAAAGATATATTTTTT+TGG - chr1_4:58123601-58123620 Msa0172940:intron 10.0%
!!! ATTATTTATTAATTGATAGT+TGG - chr1_4:58124744-58124763 Msa0172940:five_prime_UTR 10.0%
!! TTTCTATACATATTATTATC+TGG - chr1_4:58122286-58122305 Msa0172940:exon 15.0%
!!! AATGAATTTTATACAGTTTA+GGG + chr1_4:58122450-58122469 None:intergenic 15.0%
!!! TAATGAATTTTATACAGTTT+AGG + chr1_4:58122451-58122470 None:intergenic 15.0%
!!! TCAATGTAGAATTTTTATTT+TGG + chr1_4:58124560-58124579 None:intergenic 15.0%
!!! TTTAATTTGTTTTCTCGATT+AGG - chr1_4:58122154-58122173 Msa0172940:three_prime_UTR 20.0%
!! ACAATAATAATTAACAAACC+TGG + chr1_4:58122266-58122285 None:intergenic 20.0%
!!! ACAGATTTTCATATACTTTT+GGG - chr1_4:58122508-58122527 Msa0172940:intron 20.0%
!!! TAGAGTAGTTAATTAATTTG+AGG - chr1_4:58122775-58122794 Msa0172940:intron 20.0%
!!! TTCAAGTTAGAATCTATTTT+GGG - chr1_4:58122838-58122857 Msa0172940:intron 20.0%
!!! TTTTTGAGTTATTTTGTATC+AGG - chr1_4:58124298-58124317 Msa0172940:intron 20.0%
!!! TCATTTTTGCTCATTTTAAA+AGG - chr1_4:58124647-58124666 Msa0172940:intron 20.0%
!!! CAACTATTGATTTATATATG+AGG - chr1_4:58124703-58124722 Msa0172940:CDS 20.0%
!! TTTGGGCTTTATATTGTATT+GGG + chr1_4:58121915-58121934 None:intergenic 25.0%
!!! TTTTGGGCTTTATATTGTAT+TGG + chr1_4:58121916-58121935 None:intergenic 25.0%
!! ATAAGAGACTCAAGATTTTT+GGG + chr1_4:58121932-58121951 None:intergenic 25.0%
! AGGAAATTGATTGATTGATT+TGG + chr1_4:58122085-58122104 None:intergenic 25.0%
!!! TTTCGTTCTAAGATCTTAAT+AGG + chr1_4:58122485-58122504 None:intergenic 25.0%
!! GACAGATTTTCATATACTTT+TGG - chr1_4:58122507-58122526 Msa0172940:intron 25.0%
!!! CTTCAAGTTAGAATCTATTT+TGG - chr1_4:58122837-58122856 Msa0172940:intron 25.0%
!! TCAAGTTAGAATCTATTTTG+GGG - chr1_4:58122839-58122858 Msa0172940:intron 25.0%
!! AACACAATTTTTCTATGACT+TGG + chr1_4:58123078-58123097 None:intergenic 25.0%
! AATAAAAGGATACACTTCAA+GGG + chr1_4:58123275-58123294 None:intergenic 25.0%
! AAATAAAAGGATACACTTCA+AGG + chr1_4:58123276-58123295 None:intergenic 25.0%
! ATCTGAGAATTGGAAATAAA+AGG + chr1_4:58123289-58123308 None:intergenic 25.0%
!!! TTTTATTTCCAATTCTCAGA+TGG - chr1_4:58123288-58123307 Msa0172940:intron 25.0%
!! TTTCTTTTTCTTATTGTCCT+TGG - chr1_4:58123433-58123452 Msa0172940:intron 25.0%
! GAATTATCAGTATTCAATTG+AGG + chr1_4:58123531-58123550 None:intergenic 25.0%
! AAGTTGCATTACTAATTAGA+TGG - chr1_4:58123626-58123645 Msa0172940:intron 25.0%
! AGTTGCATTACTAATTAGAT+GGG - chr1_4:58123627-58123646 Msa0172940:intron 25.0%
!! TATACAGTGTTGTTAAACAT+CGG - chr1_4:58123678-58123697 Msa0172940:intron 25.0%
! GAACTAAAAAATTGTATTGG+AGG - chr1_4:58123960-58123979 Msa0172940:intron 25.0%
! TAAAAAATTGTATTGGAGGA+GGG - chr1_4:58123964-58123983 Msa0172940:intron 25.0%
! TCAGCTAGTATAAAGAATTT+CGG + chr1_4:58124449-58124468 None:intergenic 25.0%
! ATTGAAATATTTACTTGCCA+GGG - chr1_4:58124575-58124594 Msa0172940:intron 25.0%
! ATGAGGTGAAACTTATTATT+TGG - chr1_4:58124826-58124845 Msa0172940:five_prime_UTR 25.0%
! GATAAGAGACTCAAGATTTT+TGG + chr1_4:58121933-58121952 None:intergenic 30.0%
TTGGAAATTTACCAACAGTA+CGG + chr1_4:58122066-58122085 None:intergenic 30.0%
!!! TTAACACTGGTTTTCATGTT+AGG + chr1_4:58122232-58122251 None:intergenic 30.0%
! TGTTTGATTATTTTCGTTGC+AGG - chr1_4:58122373-58122392 Msa0172940:CDS 30.0%
!! AGATTGTGAAGTTTTGATGA+GGG - chr1_4:58122616-58122635 Msa0172940:intron 30.0%
! TTTTGATGAGGGTTACTATA+GGG - chr1_4:58122627-58122646 Msa0172940:intron 30.0%
TTTAATGAAGCAAGTCATCA+TGG + chr1_4:58122666-58122685 None:intergenic 30.0%
AAAGTGAAAACTACAGCAAA+AGG + chr1_4:58122690-58122709 None:intergenic 30.0%
!!! AGAATTGGTTTTGAATGGAT+TGG - chr1_4:58122731-58122750 Msa0172940:intron 30.0%
!!! TTCTTTTTAGAGTCCTCAAT+TGG - chr1_4:58122972-58122991 Msa0172940:intron 30.0%
!!! TCTTTTTAGAGTCCTCAATT+GGG - chr1_4:58122973-58122992 Msa0172940:intron 30.0%
! GGGTAAGAAATTCTTCTTTT+TGG - chr1_4:58123006-58123025 Msa0172940:intron 30.0%
TCATGGCAAAATGAAAGTAA+GGG + chr1_4:58123241-58123260 None:intergenic 30.0%
TTCATGGCAAAATGAAAGTA+AGG + chr1_4:58123242-58123261 None:intergenic 30.0%
ATAAAAGGATACACTTCAAG+GGG + chr1_4:58123274-58123293 None:intergenic 30.0%
! GCAATTGATGTTCATTTTCA+CGG - chr1_4:58123396-58123415 Msa0172940:intron 30.0%
! CAATTGATGTTCATTTTCAC+GGG - chr1_4:58123397-58123416 Msa0172940:intron 30.0%
TTTCATATAACAAAGAGCCA+AGG + chr1_4:58123453-58123472 None:intergenic 30.0%
ATAAACACCGATCAATTGTT+TGG - chr1_4:58123473-58123492 Msa0172940:intron 30.0%
ATAATCGCCAAACAATTGAT+CGG + chr1_4:58123483-58123502 None:intergenic 30.0%
ATGACAACACTTAATCTCTT+TGG + chr1_4:58123583-58123602 None:intergenic 30.0%
! GTGTCATATTTGAAACACTA+AGG - chr1_4:58123795-58123814 Msa0172940:intron 30.0%
TGGAAGAAATAGAGGAAAAT+GGG - chr1_4:58123879-58123898 Msa0172940:intron 30.0%
!!! ATTTTTTAGTTCCCCAAATG+AGG + chr1_4:58123952-58123971 None:intergenic 30.0%
GGGGAACTAAAAAATTGTAT+TGG - chr1_4:58123957-58123976 Msa0172940:intron 30.0%
CTAAAAAATTGTATTGGAGG+AGG - chr1_4:58123963-58123982 Msa0172940:intron 30.0%
GTGGAAAATATGAGGAAAAA+TGG + chr1_4:58124137-58124156 None:intergenic 30.0%
AAAGTGAAGTGGAAAATATG+AGG + chr1_4:58124242-58124261 None:intergenic 30.0%
!!! TTTTGCAGTATAATTTGCTG+TGG - chr1_4:58124523-58124542 Msa0172940:CDS 30.0%
CATTGAAATATTTACTTGCC+AGG - chr1_4:58124574-58124593 Msa0172940:intron 30.0%
AACAAGAAATATATTCTCCC+TGG + chr1_4:58124595-58124614 None:intergenic 30.0%
TTTCTTGTTGCAAATTGCAT+TGG - chr1_4:58124606-58124625 Msa0172940:intron 30.0%
ACATTATTGCTTGCTTGCTT+AGG + chr1_4:58121988-58122007 None:intergenic 35.0%
AAGCAAGCAAGCAATAATGT+CGG - chr1_4:58121988-58122007 Msa0172940:three_prime_UTR 35.0%
TTTCCTGAGAGATTCGATTT+GGG + chr1_4:58122023-58122042 None:intergenic 35.0%
ATTTCCTGAGAGATTCGATT+TGG + chr1_4:58122024-58122043 None:intergenic 35.0%
AAATCGAATCTCTCAGGAAA+TGG - chr1_4:58122023-58122042 Msa0172940:three_prime_UTR 35.0%
AATCGAATCTCTCAGGAAAT+GGG - chr1_4:58122024-58122043 Msa0172940:three_prime_UTR 35.0%
AATTTCGTTGCATTGCATGA+AGG - chr1_4:58122349-58122368 Msa0172940:CDS 35.0%
ATTTCGTTGCATTGCATGAA+GGG - chr1_4:58122350-58122369 Msa0172940:CDS 35.0%
!! GAGATTGTGAAGTTTTGATG+AGG - chr1_4:58122615-58122634 Msa0172940:intron 35.0%
!! GTTTTGATGAGGGTTACTAT+AGG - chr1_4:58122626-58122645 Msa0172940:intron 35.0%
!! AGTTTTCACTTTTGCCTGAT+TGG - chr1_4:58122698-58122717 Msa0172940:intron 35.0%
!!! GGTTTTGAATGGATTGGTTT+TGG - chr1_4:58122737-58122756 Msa0172940:intron 35.0%
!!! CTTTTTAGAGTCCTCAATTG+GGG - chr1_4:58122974-58122993 Msa0172940:intron 35.0%
!!! TGCGTTGTAGAAGCTTTTTT+TGG - chr1_4:58123036-58123055 Msa0172940:intron 35.0%
GAAAAATTGTGTTTCGCAAC+AGG - chr1_4:58123085-58123104 Msa0172940:intron 35.0%
! TGTTGTTTGATTCTAGTGCA+TGG - chr1_4:58123328-58123347 Msa0172940:intron 35.0%
CATTACTAATTAGATGGGCA+TGG - chr1_4:58123632-58123651 Msa0172940:intron 35.0%
TAATTAGATGGGCATGGAAA+TGG - chr1_4:58123638-58123657 Msa0172940:intron 35.0%
TGGAAATGGAGTGTAAAACA+AGG - chr1_4:58123652-58123671 Msa0172940:intron 35.0%
GTAAACTCTGCTACATAACA+CGG + chr1_4:58123742-58123761 None:intergenic 35.0%
TTTCCAATCACGGATAATAG+CGG + chr1_4:58123770-58123789 None:intergenic 35.0%
GGAAAAAAGGAATGAACAAG+TGG - chr1_4:58123859-58123878 Msa0172940:intron 35.0%
TGAACAAGTGGAAGAAATAG+AGG - chr1_4:58123871-58123890 Msa0172940:intron 35.0%
GTGGAAGAAATAGAGGAAAA+TGG - chr1_4:58123878-58123897 Msa0172940:intron 35.0%
AAGAAATAGAGGAAAATGGG+AGG - chr1_4:58123882-58123901 Msa0172940:intron 35.0%
AGAAATAGAGGAAAATGGGA+GGG - chr1_4:58123883-58123902 Msa0172940:intron 35.0%
AATACTAAACCCTCCTCATT+TGG - chr1_4:58123936-58123955 Msa0172940:intron 35.0%
ATACTAAACCCTCCTCATTT+GGG - chr1_4:58123937-58123956 Msa0172940:intron 35.0%
TTTGTGGAGTGGAAAATATG+AGG + chr1_4:58124145-58124164 None:intergenic 35.0%
!! TAAGGCTTTGTTTGTGAGTT+TGG + chr1_4:58124188-58124207 None:intergenic 35.0%
AACTCACAAACAAAGCCTTA+AGG - chr1_4:58124188-58124207 Msa0172940:intron 35.0%
ACAAAGCCTTAAGGATATGA+TGG - chr1_4:58124197-58124216 Msa0172940:intron 35.0%
CAAAGCCTTAAGGATATGAT+GGG - chr1_4:58124198-58124217 Msa0172940:intron 35.0%
ACAAACCCATCATATCCTTA+AGG + chr1_4:58124206-58124225 None:intergenic 35.0%
TGATCACAAGTCTGAAGAAA+AGG - chr1_4:58124380-58124399 Msa0172940:intron 35.0%
!! AATTTGCTGTGGTTCATCAA+TGG - chr1_4:58124534-58124553 Msa0172940:intron 35.0%
ACCACTTCATTTGTAACACT+TGG - chr1_4:58124861-58124880 Msa0172940:five_prime_UTR 35.0%
CCACTTCATTTGTAACACTT+GGG - chr1_4:58124862-58124881 Msa0172940:five_prime_UTR 35.0%
TGGGAAATACTATTGCGTGA+GGG + chr1_4:58121896-58121915 None:intergenic 40.0%
TTGGGAAATACTATTGCGTG+AGG + chr1_4:58121897-58121916 None:intergenic 40.0%
AAACCCAAATCGAATCTCTC+AGG - chr1_4:58122017-58122036 Msa0172940:three_prime_UTR 40.0%
! TTTCTCGATTAGGTTGCTTG+TGG - chr1_4:58122164-58122183 Msa0172940:three_prime_UTR 40.0%
GGCGTGAATAATCTTAACAC+TGG + chr1_4:58122245-58122264 None:intergenic 40.0%
GTGTTAAGATTATTCACGCC+AGG - chr1_4:58122245-58122264 Msa0172940:three_prime_UTR 40.0%
!! ACTTTTGCCTGATTGGTTCT+GGG - chr1_4:58122705-58122724 Msa0172940:intron 40.0%
!!! GGACGAGAATTGGTTTTGAA+TGG - chr1_4:58122726-58122745 Msa0172940:intron 40.0%
CTGGTTGAGCGAATAAATCA+AGG + chr1_4:58123150-58123169 None:intergenic 40.0%
CTTGATTTATTCGCTCAACC+AGG - chr1_4:58123148-58123167 Msa0172940:intron 40.0%
GCATGGCAAAACGAAAGTAA+GGG + chr1_4:58123188-58123207 None:intergenic 40.0%
TGCATGGCAAAACGAAAGTA+AGG + chr1_4:58123189-58123208 None:intergenic 40.0%
CAAGAGAAAACTCGAATGCA+TGG + chr1_4:58123205-58123224 None:intergenic 40.0%
ATGAAAGTAAGGGAGATGTG+AGG + chr1_4:58123231-58123250 None:intergenic 40.0%
CAAGGGGAAACTTGAATTCA+TGG + chr1_4:58123258-58123277 None:intergenic 40.0%
TCCAATTCTCAGATGGCAAT+TGG - chr1_4:58123295-58123314 Msa0172940:intron 40.0%
TCCAATTGCCATCTGAGAAT+TGG + chr1_4:58123299-58123318 None:intergenic 40.0%
CAGTATTCAATTGAGGAGAC+AGG + chr1_4:58123524-58123543 None:intergenic 40.0%
AAACCGCTATTATCCGTGAT+TGG - chr1_4:58123764-58123783 Msa0172940:intron 40.0%
TTATCCGTGATTGGAAACAC+TGG - chr1_4:58123773-58123792 Msa0172940:intron 40.0%
!! TAAGGCTTTGTTTGCGAGTT+TGG - chr1_4:58123813-58123832 Msa0172940:intron 40.0%
GAAATAGAGGAAAATGGGAG+GGG - chr1_4:58123884-58123903 Msa0172940:intron 40.0%
TACTAAACCCTCCTCATTTG+GGG - chr1_4:58123938-58123957 Msa0172940:intron 40.0%
! TTTAGTTCCCCAAATGAGGA+GGG + chr1_4:58123948-58123967 None:intergenic 40.0%
!!! TTTTAGTTCCCCAAATGAGG+AGG + chr1_4:58123949-58123968 None:intergenic 40.0%
! CTTTGTTTGTGAGTTTGGAG+GGG + chr1_4:58124183-58124202 None:intergenic 40.0%
!! GCTTTGTTTGTGAGTTTGGA+GGG + chr1_4:58124184-58124203 None:intergenic 40.0%
CAGCACAAGAAAAAGTGAAG+TGG + chr1_4:58124253-58124272 None:intergenic 40.0%
ACGCAAGTTCTTTAACTCCA+CGG - chr1_4:58124801-58124820 Msa0172940:five_prime_UTR 40.0%
CCCAAGTGTTACAAATGAAG+TGG + chr1_4:58124865-58124884 None:intergenic 40.0%
!! TGTAAACCTGCTTGATGACG+CGG + chr1_4:58121837-58121856 None:intergenic 45.0%
TTGCTTGCTTGCTTAGGAGA+GGG + chr1_4:58121982-58122001 None:intergenic 45.0%
ATTGCTTGCTTGCTTAGGAG+AGG + chr1_4:58121983-58122002 None:intergenic 45.0%
GAACACAAACTCCGTACTGT+TGG - chr1_4:58122052-58122071 Msa0172940:three_prime_UTR 45.0%
! TTAGGTTGCTTGTGGCTTAG+CGG - chr1_4:58122172-58122191 Msa0172940:three_prime_UTR 45.0%
CTTGTGGCTTAGCGGTATTA+CGG - chr1_4:58122180-58122199 Msa0172940:three_prime_UTR 45.0%
TTGTGGCTTAGCGGTATTAC+GGG - chr1_4:58122181-58122200 Msa0172940:three_prime_UTR 45.0%
CGGGTTCAATTGCGTACAAT+TGG - chr1_4:58122200-58122219 Msa0172940:three_prime_UTR 45.0%
!! CACTTTTGCCTGATTGGTTC+TGG - chr1_4:58122704-58122723 Msa0172940:intron 45.0%
!! ATTGGTTCTGGGACGAGAAT+TGG - chr1_4:58122716-58122735 Msa0172940:intron 45.0%
CTCAATTGGGGTAGTTGGAA+GGG - chr1_4:58122986-58123005 Msa0172940:intron 45.0%
TGAAACAATGCTGACGTGCT+TGG - chr1_4:58123374-58123393 Msa0172940:intron 45.0%
GACACCAGTGTTTCCAATCA+CGG + chr1_4:58123780-58123799 None:intergenic 45.0%
!! GCTTTGTTTGCGAGTTTGGA+GGG - chr1_4:58123817-58123836 Msa0172940:intron 45.0%
! CTTTGTTTGCGAGTTTGGAG+GGG - chr1_4:58123818-58123837 Msa0172940:intron 45.0%
AGGGGAAGGTTTGGGAAAAA+AGG - chr1_4:58123846-58123865 Msa0172940:intron 45.0%
! TGTATTGGAGGAGGGTTTTG+AGG - chr1_4:58123972-58123991 Msa0172940:intron 45.0%
!! GTATTGGAGGAGGGTTTTGA+GGG - chr1_4:58123973-58123992 Msa0172940:intron 45.0%
!!! TATTGGAGGAGGGTTTTGAG+GGG - chr1_4:58123974-58123993 Msa0172940:intron 45.0%
!! GGCTTTGTTTGTGAGTTTGG+AGG + chr1_4:58124185-58124204 None:intergenic 45.0%
CATGCTCAAGCTCTTACACT+GGG - chr1_4:58124324-58124343 Msa0172940:intron 45.0%
AGTCTGAAGAAAAGGCTGCA+AGG - chr1_4:58124388-58124407 Msa0172940:intron 45.0%
GTCTGAAGAAAAGGCTGCAA+GGG - chr1_4:58124389-58124408 Msa0172940:intron 45.0%
AAAAGGCTGCAAGGGATTCT+AGG - chr1_4:58124397-58124416 Msa0172940:intron 45.0%
GTATCTGACATCTCACAGCT+TGG - chr1_4:58124474-58124493 Msa0172940:CDS 45.0%
GTGGAGTTAAAGAACTTGCG+TGG + chr1_4:58124802-58124821 None:intergenic 45.0%
CGCAAGTTCTTTAACTCCAC+GGG - chr1_4:58124802-58124821 Msa0172940:five_prime_UTR 45.0%
GCAAGTTCTTTAACTCCACG+GGG - chr1_4:58124803-58124822 Msa0172940:five_prime_UTR 45.0%
TCATTTGTAACACTTGGGCG+TGG - chr1_4:58124867-58124886 Msa0172940:five_prime_UTR 45.0%
TTCTCGTCCCAGAACCAATC+AGG + chr1_4:58122715-58122734 None:intergenic 50.0%
GAGTCCTCAATTGGGGTAGT+TGG - chr1_4:58122981-58123000 Msa0172940:intron 50.0%
CCTTCCAACTACCCCAATTG+AGG + chr1_4:58122988-58123007 None:intergenic 50.0%
CCTCAATTGGGGTAGTTGGA+AGG - chr1_4:58122985-58123004 Msa0172940:intron 50.0%
ACGAAAGTAAGGGAGATGCG+AGG + chr1_4:58123178-58123197 None:intergenic 50.0%
!! GGCTTTGTTTGCGAGTTTGG+AGG - chr1_4:58123816-58123835 Msa0172940:intron 50.0%
TTTGCGAGTTTGGAGGGGAA+GGG - chr1_4:58123823-58123842 Msa0172940:intron 50.0%
ATAGAGGAAAATGGGAGGGG+AGG - chr1_4:58123887-58123906 Msa0172940:intron 50.0%
TAGAGGAAAATGGGAGGGGA+GGG - chr1_4:58123888-58123907 Msa0172940:intron 50.0%
!!! AGGAGGGTTTTGAGGGGTTT+TGG - chr1_4:58123980-58123999 Msa0172940:intron 50.0%
!!! AGGGTTTTGAGGGGTTTTGG+AGG - chr1_4:58123983-58124002 Msa0172940:intron 50.0%
!!! GGGTTTTGAGGGGTTTTGGA+GGG - chr1_4:58123984-58124003 Msa0172940:intron 50.0%
! GAAGGAAAGGCTTTGTGGAG+TGG + chr1_4:58124156-58124175 None:intergenic 50.0%
TTTGTGAGTTTGGAGGGGAG+GGG + chr1_4:58124178-58124197 None:intergenic 50.0%
GTTTGTGAGTTTGGAGGGGA+GGG + chr1_4:58124179-58124198 None:intergenic 50.0%
TGTTTGTGAGTTTGGAGGGG+AGG + chr1_4:58124180-58124199 None:intergenic 50.0%
GCATGCTCAAGCTCTTACAC+TGG - chr1_4:58124323-58124342 Msa0172940:intron 50.0%
!! CTTACACTGGGAGCATTAGC+TGG - chr1_4:58124336-58124355 Msa0172940:intron 50.0%
TCTTTAACTCCACGGGGATG+AGG - chr1_4:58124809-58124828 Msa0172940:five_prime_UTR 50.0%
TAAGTTTCACCTCATCCCCG+TGG + chr1_4:58124821-58124840 None:intergenic 50.0%
TGACGCGGCGGAACTACTAT+CGG + chr1_4:58121822-58121841 None:intergenic 55.0%
!! AAACCTGCTTGATGACGCGG+CGG + chr1_4:58121834-58121853 None:intergenic 55.0%
AGGGAGATGCGAGGTATACC+TGG + chr1_4:58123169-58123188 None:intergenic 55.0%
GTTTGCGAGTTTGGAGGGGA+AGG - chr1_4:58123822-58123841 Msa0172940:intron 55.0%
AAAATGGGAGGGGAGGGCTT+TGG - chr1_4:58123894-58123913 Msa0172940:intron 55.0%
AAATGGGAGGGGAGGGCTTT+GGG - chr1_4:58123895-58123914 Msa0172940:intron 55.0%
GAGGGGAAGGAAAGGCTTTG+TGG + chr1_4:58124161-58124180 None:intergenic 55.0%
AGCATTAGCTGGAGCTGCAG+TGG - chr1_4:58124347-58124366 Msa0172940:intron 55.0%
CGAGTTTGGAGGGGAAGGGA+GGG - chr1_4:58123827-58123846 Msa0172940:intron 60.0%
GAGTTTGGAGGGGAAGGGAG+GGG - chr1_4:58123828-58123847 Msa0172940:intron 60.0%
GGGAAGGGAGGGGAAGGTTT+GGG - chr1_4:58123838-58123857 Msa0172940:intron 60.0%
AATGGGAGGGGAGGGCTTTG+GGG - chr1_4:58123896-58123915 Msa0172940:intron 60.0%
TTGGAGGGGAGGGGAAGGAA+AGG + chr1_4:58124169-58124188 None:intergenic 60.0%
TGAGTTTGGAGGGGAGGGGA+AGG + chr1_4:58124174-58124193 None:intergenic 60.0%
GTTCCGCCGCGTCATCAAGC+AGG - chr1_4:58121828-58121847 Msa0172940:three_prime_UTR 65.0%
GCGAGTTTGGAGGGGAAGGG+AGG - chr1_4:58123826-58123845 Msa0172940:intron 65.0%
TTGGAGGGGAAGGGAGGGGA+AGG - chr1_4:58123832-58123851 Msa0172940:intron 65.0%
GGGGAAGGGAGGGGAAGGTT+TGG - chr1_4:58123837-58123856 Msa0172940:intron 65.0%
! ATGGGAGGGGAGGGCTTTGG+GGG - chr1_4:58123897-58123916 Msa0172940:intron 65.0%
!! TGGGAGGGGAGGGCTTTGGG+GGG - chr1_4:58123898-58123917 Msa0172940:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 58121818 58124908 58121818 ID=Msa0172940;Name=Msa0172940
chr1_4 mRNA 58121818 58124908 58121818 ID=Msa0172940-mRNA-1;Parent=Msa0172940;Name=Msa0172940-mRNA-1;_AED=0.05;_eAED=0.05;_QI=186|1|1|1|1|1|3|483|88
chr1_4 exon 58121818 58122406 58121818 ID=Msa0172940-mRNA-1:exon:17911;Parent=Msa0172940-mRNA-1
chr1_4 exon 58124460 58124550 58124460 ID=Msa0172940-mRNA-1:exon:17910;Parent=Msa0172940-mRNA-1
chr1_4 exon 58124653 58124908 58124653 ID=Msa0172940-mRNA-1:exon:17909;Parent=Msa0172940-mRNA-1
chr1_4 five_prime_UTR 58124723 58124908 58124723 ID=Msa0172940-mRNA-1:five_prime_utr;Parent=Msa0172940-mRNA-1
chr1_4 CDS 58124653 58124722 58124653 ID=Msa0172940-mRNA-1:cds;Parent=Msa0172940-mRNA-1
chr1_4 CDS 58124460 58124550 58124460 ID=Msa0172940-mRNA-1:cds;Parent=Msa0172940-mRNA-1
chr1_4 CDS 58122301 58122406 58122301 ID=Msa0172940-mRNA-1:cds;Parent=Msa0172940-mRNA-1
chr1_4 three_prime_UTR 58121818 58122300 58121818 ID=Msa0172940-mRNA-1:three_prime_utr;Parent=Msa0172940-mRNA-1
Gene Sequence

>Msa0172940

ATGTCGGATGCTAAAACCCAAATCGAATCTCTCAGGAAATGGGTCGTTGAACACAAACTCCGTACTGTTGGTTGCTTGTGGCTTAGCGGTATTACGGGTTCAATTGCGTACAATTGGTCTCGTCCTAACATGAAAACCAGTGTTAAGATTATTCACGCCAGGTTGCATGCTCAAGCTCTTACACTGGGAGCATTAGCTGGAGCTGCAGTGGTAGAATATTATGATCACAAGTCTGAAGAAAAGGCTGCAAGGGATTCTAGGCGATAA
Protein sequence

>Msa0172940

MSDAKTQIESLRKWVVEHKLRTVGCLWLSGITGSIAYNWSRPNMKTSVKIIHARLHAQALTLGALAGAAVVEYYDHKSEEKAARDSRR*