AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0114560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0114560 MtrunA17_Chr1g0192231 97.778 90 0 1 1 88 1 90 1.71e-59 176
Msa0114560 MtrunA17_Chr3g0114431 46.575 73 38 1 8 79 1 73 1.57e-18 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0114560 AT3G05550.1 83.544 79 13 0 1 79 1 79 8.33e-34 112
Msa0114560 AT5G27760.1 78.205 78 17 0 1 78 1 78 1.18e-31 106
Msa0114560 AT5G27760.2 72.222 54 15 0 1 54 1 54 6.87e-27 94.0

Find 52 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGGTGAAACTTATTATT+TGG 0.155300 1_3:+31762029 Msa0114560:three_prime_UTR
TTTAGGCTTTATATTGTATT+GGG 0.296227 1_3:-31759136 None:intergenic
ATTTCCTGAGAGATTCGATT+TGG 0.327609 1_3:-31759245 None:intergenic
AAATCGAATCTCTCAGGAAA+TGG 0.335949 1_3:+31759247 Msa0114560:CDS
AAAAGGCTGCAAGGGATTCT+AGG 0.341872 1_3:+31761600 Msa0114560:CDS
TTGTGGCTTAGCGGTATTAC+GGG 0.371274 1_3:+31759408 Msa0114560:CDS
TTTGTGGCTTAGCGGTATTA+CGG 0.376845 1_3:+31759407 Msa0114560:CDS
ACATTATTGCTTGCTTGCTT+AGG 0.385386 1_3:-31759209 None:intergenic
CGGGTTCAATTGCGTACAAT+TGG 0.387745 1_3:+31759427 Msa0114560:CDS
ATTATTTATTAATTGATAGT+TGG 0.403725 1_3:+31761947 Msa0114560:three_prime_UTR
CATTGAAATATTTACTTGCC+AGG 0.410212 1_3:+31761777 Msa0114560:three_prime_UTR
ACCACTTCATTTGTAACACT+TGG 0.413631 1_3:+31762064 Msa0114560:three_prime_UTR
CGCAAGTTCTTTAACTCCAC+GGG 0.423820 1_3:+31762005 Msa0114560:three_prime_UTR
CTTACACTGGGAGCATTAGC+TGG 0.431990 1_3:+31761539 Msa0114560:CDS
TTTCCTGAGAGATTCGATTT+GGG 0.436962 1_3:-31759244 None:intergenic
TTTCTTGTTGCAAATTGCAT+TGG 0.444422 1_3:+31761809 Msa0114560:three_prime_UTR
TGATCACAAGTCTGAAGAAA+AGG 0.467242 1_3:+31761583 Msa0114560:CDS
TTTCTCGATTAGGTTGTTTG+TGG 0.469318 1_3:+31759391 Msa0114560:intron
AATTTGCTGTGGTTCATCAA+TGG 0.470506 1_3:+31761737 Msa0114560:three_prime_UTR
AATCGAATCTCTCAGGAAAT+GGG 0.471588 1_3:+31759248 Msa0114560:CDS
CAACTATTGATTTATATATG+AGG 0.481886 1_3:+31761906 Msa0114560:three_prime_UTR
AAACCCAAATCGAATCTCTC+AGG 0.502597 1_3:+31759241 Msa0114560:CDS
TCTTTAACTCCACGGGGATG+AGG 0.522631 1_3:+31762012 Msa0114560:three_prime_UTR
GCATGCTCAAGCTCTTACAC+TGG 0.522809 1_3:+31761526 Msa0114560:CDS
GTCTGAAGAAAAGGCTGCAA+GGG 0.524443 1_3:+31761592 Msa0114560:CDS
TTAGGTTGTTTGTGGCTTAG+CGG 0.532960 1_3:+31759399 Msa0114560:intron
CCACTTCATTTGTAACACTT+GGG 0.535710 1_3:+31762065 Msa0114560:three_prime_UTR
GTGTTAAGATTATTCACGCC+AGG 0.537077 1_3:+31759472 Msa0114560:CDS
AACAAGAAATATATTCTCCC+TGG 0.539373 1_3:-31761795 None:intergenic
ATTGAAATATTTACTTGCCA+GGG 0.543373 1_3:+31761778 Msa0114560:three_prime_UTR
GCGTCATCAAGCAACGAAGC+AGG 0.552051 1_3:+31759052 Msa0114560:five_prime_UTR
GTATCTGACATCTCACAGCT+TGG 0.565513 1_3:+31761677 Msa0114560:three_prime_UTR
TTGGAAATTTACCAACAGTA+CGG 0.567029 1_3:-31759287 None:intergenic
TTGCTTGCTTGCTTAGGAGA+GGG 0.573235 1_3:-31759203 None:intergenic
AGCATTAGCTGGAGCTGCAG+TGG 0.578637 1_3:+31761550 Msa0114560:CDS
CCCAAGTGTTACAAATGAAG+TGG 0.581164 1_3:-31762065 None:intergenic
CATGCTCAAGCTCTTACACT+GGG 0.582325 1_3:+31761527 Msa0114560:CDS
GAACACAAACTCCGTACTGT+TGG 0.585849 1_3:+31759276 Msa0114560:CDS
AGTCTGAAGAAAAGGCTGCA+AGG 0.589757 1_3:+31761591 Msa0114560:CDS
ACGCAAGTTCTTTAACTCCA+CGG 0.590810 1_3:+31762004 Msa0114560:three_prime_UTR
TGCTTCGTTGCTTGATGACG+CGG 0.593098 1_3:-31759050 None:intergenic
TGACGCGGCGGAACTACTAT+CGG 0.597040 1_3:-31759035 None:intergenic
TCATTTGTAACACTTGGGCG+TGG 0.598299 1_3:+31762070 Msa0114560:three_prime_UTR
ATTGCTTGCTTGCTTAGGAG+AGG 0.609965 1_3:-31759204 None:intergenic
TTGGGAAATACTATTGCGTG+AGG 0.611327 1_3:-31759118 None:intergenic
GGCGTGAATAATCTTAACAC+TGG 0.626108 1_3:-31759469 None:intergenic
AAGCAAGCAAGCAATAATGT+CGG 0.649556 1_3:+31759212 Msa0114560:exon
TTCGTTGCTTGATGACGCGG+CGG 0.650416 1_3:-31759047 None:intergenic
GTGGAGTTAAAGAACTTGCG+TGG 0.662404 1_3:-31762002 None:intergenic
TGGGAAATACTATTGCGTGA+GGG 0.685241 1_3:-31759117 None:intergenic
TAAGTTTCACCTCATCCCCG+TGG 0.693295 1_3:-31762021 None:intergenic
GCAAGTTCTTTAACTCCACG+GGG 0.741920 1_3:+31762006 Msa0114560:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTATTATTATTATTT+TGG + chr1_3:31759790-31759809 Msa0114560:intron 0.0%
!!! ATTGAAAAGATATATTTTTT+TGG + chr1_3:31760803-31760822 Msa0114560:intron 10.0%
!!! ATTATTTATTAATTGATAGT+TGG + chr1_3:31761947-31761966 Msa0114560:three_prime_UTR 10.0%
!! ATCATATCATCATAAAATAA+AGG - chr1_3:31759329-31759348 None:intergenic 15.0%
!!! ATTTTATGATGATATGATTT+AGG + chr1_3:31759331-31759350 Msa0114560:intron 15.0%
!!! AATGAATTTTATACAGTTTA+GGG - chr1_3:31759674-31759693 None:intergenic 15.0%
!!! TAATGAATTTTATACAGTTT+AGG - chr1_3:31759675-31759694 None:intergenic 15.0%
!!! TCAATGTAGAATTTTTATTT+TGG - chr1_3:31761763-31761782 None:intergenic 15.0%
!!! TTTAGGCTTTATATTGTATT+GGG - chr1_3:31759139-31759158 None:intergenic 20.0%
!!! TTTTAGGCTTTATATTGTAT+TGG - chr1_3:31759140-31759159 None:intergenic 20.0%
!!! TTTAATTTGTTTTCTCGATT+AGG + chr1_3:31759381-31759400 Msa0114560:intron 20.0%
!! ACAATAATAATTAACAAACC+TGG - chr1_3:31759493-31759512 None:intergenic 20.0%
!!! TGTTTTCTATACATATTATC+TGG + chr1_3:31759510-31759529 Msa0114560:intron 20.0%
!!! TTTAGTTCTAAGATCTTAAT+AGG - chr1_3:31759709-31759728 None:intergenic 20.0%
!!! ACAGATTTTCATATACTTTT+GGG + chr1_3:31759732-31759751 Msa0114560:intron 20.0%
!!! TAGAGTAGTTAATTAATTTG+AGG + chr1_3:31759999-31760018 Msa0114560:intron 20.0%
!!! TTCAAGTTAGAATCTATTTT+GGG + chr1_3:31760062-31760081 Msa0114560:intron 20.0%
!!! TTTTTGAGTTATTTTGTATC+AGG + chr1_3:31761501-31761520 Msa0114560:intron 20.0%
!! TCAGCTAGTATAAAAAATTT+CGG - chr1_3:31761652-31761671 None:intergenic 20.0%
!!! TCATTTTTGCTCATTTTAAA+AGG + chr1_3:31761850-31761869 Msa0114560:three_prime_UTR 20.0%
!!! CAACTATTGATTTATATATG+AGG + chr1_3:31761906-31761925 Msa0114560:three_prime_UTR 20.0%
!! AAAAGAAGAAAAGTTTTACG+AGG - chr1_3:31759178-31759197 None:intergenic 25.0%
! AGGAAATTGATTGATTGATT+TGG - chr1_3:31759309-31759328 None:intergenic 25.0%
!! GACAGATTTTCATATACTTT+TGG + chr1_3:31759731-31759750 Msa0114560:intron 25.0%
!!! CTTCAAGTTAGAATCTATTT+TGG + chr1_3:31760061-31760080 Msa0114560:intron 25.0%
!! TCAAGTTAGAATCTATTTTG+GGG + chr1_3:31760063-31760082 Msa0114560:intron 25.0%
!! AACACAATTTTTCTATGACT+TGG - chr1_3:31760302-31760321 None:intergenic 25.0%
! AATAAAAGGATACACTTCAA+GGG - chr1_3:31760477-31760496 None:intergenic 25.0%
! AAATAAAAGGATACACTTCA+AGG - chr1_3:31760478-31760497 None:intergenic 25.0%
! ATCTGAGAATTGGAAATAAA+AGG - chr1_3:31760491-31760510 None:intergenic 25.0%
!!! TTTTATTTCCAATTCTCAGA+TGG + chr1_3:31760490-31760509 Msa0114560:intron 25.0%
!! TTTCTTTTTCTTATTGTCCT+TGG + chr1_3:31760635-31760654 Msa0114560:intron 25.0%
! GAATTATCAGTATTCAATTG+AGG - chr1_3:31760733-31760752 None:intergenic 25.0%
! AAGTTGCATTACTAATTAGA+TGG + chr1_3:31760828-31760847 Msa0114560:intron 25.0%
! AGTTGCATTACTAATTAGAT+GGG + chr1_3:31760829-31760848 Msa0114560:intron 25.0%
!! TATACAGTGTTGTTAAACAT+CGG + chr1_3:31760880-31760899 Msa0114560:intron 25.0%
! GAACTAAAAAATTGTATTGG+AGG + chr1_3:31761162-31761181 Msa0114560:intron 25.0%
! TAAAAAATTGTATTGGAGGA+GGG + chr1_3:31761166-31761185 Msa0114560:intron 25.0%
! ATTGAAATATTTACTTGCCA+GGG + chr1_3:31761778-31761797 Msa0114560:three_prime_UTR 25.0%
! ATGAGGTGAAACTTATTATT+TGG + chr1_3:31762029-31762048 Msa0114560:three_prime_UTR 25.0%
! GTAAGAGACTCAAGATTTTT+AGG - chr1_3:31759156-31759175 None:intergenic 30.0%
TTGGAAATTTACCAACAGTA+CGG - chr1_3:31759290-31759309 None:intergenic 30.0%
!!! TTAACACTGGTTTTCATGTT+AGG - chr1_3:31759459-31759478 None:intergenic 30.0%
! TGTTTGATTATTTTCGTTGC+AGG + chr1_3:31759597-31759616 Msa0114560:intron 30.0%
!! AGATTGTGAAGTTTTGATGA+GGG + chr1_3:31759840-31759859 Msa0114560:intron 30.0%
! TTTTGATGAGGGTTACTATA+GGG + chr1_3:31759851-31759870 Msa0114560:intron 30.0%
TTTAATGAAGCAAGTCATCA+TGG - chr1_3:31759890-31759909 None:intergenic 30.0%
AAAGTGAAAACTACAGCAAA+AGG - chr1_3:31759914-31759933 None:intergenic 30.0%
!!! AGAATTGGTTTTGAATGGAT+TGG + chr1_3:31759955-31759974 Msa0114560:intron 30.0%
!!! TTCTTTTTAGAGTCCTCAAT+TGG + chr1_3:31760196-31760215 Msa0114560:intron 30.0%
!!! TCTTTTTAGAGTCCTCAATT+GGG + chr1_3:31760197-31760216 Msa0114560:intron 30.0%
! GGGTAAGAAATTCTTCTTTT+TGG + chr1_3:31760230-31760249 Msa0114560:intron 30.0%
TCATGGCAAAATGAAAGTAA+GGG - chr1_3:31760443-31760462 None:intergenic 30.0%
TTCATGGCAAAATGAAAGTA+AGG - chr1_3:31760444-31760463 None:intergenic 30.0%
ATAAAAGGATACACTTCAAG+GGG - chr1_3:31760476-31760495 None:intergenic 30.0%
! GCAATTGATGTTCATTTTCA+CGG + chr1_3:31760598-31760617 Msa0114560:intron 30.0%
! CAATTGATGTTCATTTTCAC+GGG + chr1_3:31760599-31760618 Msa0114560:intron 30.0%
TTTCATATAACAAAGAGCCA+AGG - chr1_3:31760655-31760674 None:intergenic 30.0%
ATAAACACCGATCAATTGTT+TGG + chr1_3:31760675-31760694 Msa0114560:intron 30.0%
ATAATCGCCAAACAATTGAT+CGG - chr1_3:31760685-31760704 None:intergenic 30.0%
ATGACAACACTTAATCTCTT+TGG - chr1_3:31760785-31760804 None:intergenic 30.0%
! GTGTCATATTTGAAACACTA+AGG + chr1_3:31760997-31761016 Msa0114560:intron 30.0%
TGGAAGAAATAGAGGAAAAT+GGG + chr1_3:31761081-31761100 Msa0114560:intron 30.0%
!!! ATTTTTTAGTTCCCCAAATG+AGG - chr1_3:31761154-31761173 None:intergenic 30.0%
GGGGAACTAAAAAATTGTAT+TGG + chr1_3:31761159-31761178 Msa0114560:intron 30.0%
CTAAAAAATTGTATTGGAGG+AGG + chr1_3:31761165-31761184 Msa0114560:intron 30.0%
GTGGAAAATATGAGGAAAAA+TGG - chr1_3:31761339-31761358 None:intergenic 30.0%
AAAGTGAAGTGGAAAATATG+AGG - chr1_3:31761445-31761464 None:intergenic 30.0%
!!! TTTTGCAGTATAATTTGCTG+TGG + chr1_3:31761726-31761745 Msa0114560:three_prime_UTR 30.0%
CATTGAAATATTTACTTGCC+AGG + chr1_3:31761777-31761796 Msa0114560:three_prime_UTR 30.0%
AACAAGAAATATATTCTCCC+TGG - chr1_3:31761798-31761817 None:intergenic 30.0%
TTTCTTGTTGCAAATTGCAT+TGG + chr1_3:31761809-31761828 Msa0114560:three_prime_UTR 30.0%
ACATTATTGCTTGCTTGCTT+AGG - chr1_3:31759212-31759231 None:intergenic 35.0%
AAGCAAGCAAGCAATAATGT+CGG + chr1_3:31759212-31759231 Msa0114560:exon 35.0%
TTTCCTGAGAGATTCGATTT+GGG - chr1_3:31759247-31759266 None:intergenic 35.0%
ATTTCCTGAGAGATTCGATT+TGG - chr1_3:31759248-31759267 None:intergenic 35.0%
AAATCGAATCTCTCAGGAAA+TGG + chr1_3:31759247-31759266 Msa0114560:CDS 35.0%
AATCGAATCTCTCAGGAAAT+GGG + chr1_3:31759248-31759267 Msa0114560:CDS 35.0%
! TTTCTCGATTAGGTTGTTTG+TGG + chr1_3:31759391-31759410 Msa0114560:intron 35.0%
AATTTCGTTGCATTGCATGA+AGG + chr1_3:31759573-31759592 Msa0114560:intron 35.0%
ATTTCGTTGCATTGCATGAA+GGG + chr1_3:31759574-31759593 Msa0114560:intron 35.0%
!! GAGATTGTGAAGTTTTGATG+AGG + chr1_3:31759839-31759858 Msa0114560:intron 35.0%
!! GTTTTGATGAGGGTTACTAT+AGG + chr1_3:31759850-31759869 Msa0114560:intron 35.0%
!! AGTTTTCACTTTTGCCTGAT+TGG + chr1_3:31759922-31759941 Msa0114560:intron 35.0%
!!! GGTTTTGAATGGATTGGTTT+TGG + chr1_3:31759961-31759980 Msa0114560:intron 35.0%
!!! CTTTTTAGAGTCCTCAATTG+GGG + chr1_3:31760198-31760217 Msa0114560:intron 35.0%
!!! TGCGTTGTAGAAGCTTTTTT+TGG + chr1_3:31760260-31760279 Msa0114560:intron 35.0%
GAAAAATTGTGTTTCGCAAC+AGG + chr1_3:31760309-31760328 Msa0114560:intron 35.0%
! TGTTGTTTGATTCTAGTGCA+TGG + chr1_3:31760530-31760549 Msa0114560:intron 35.0%
CATTACTAATTAGATGGGCA+TGG + chr1_3:31760834-31760853 Msa0114560:intron 35.0%
TAATTAGATGGGCATGGAAA+TGG + chr1_3:31760840-31760859 Msa0114560:intron 35.0%
TGGAAATGGAGTGTAAAACA+AGG + chr1_3:31760854-31760873 Msa0114560:intron 35.0%
GTAAACTCTGCTACATAACA+CGG - chr1_3:31760944-31760963 None:intergenic 35.0%
TTTCCAATCACGGATAATAG+CGG - chr1_3:31760972-31760991 None:intergenic 35.0%
GGAAAAAAGGAATGAACAAG+TGG + chr1_3:31761061-31761080 Msa0114560:intron 35.0%
TGAACAAGTGGAAGAAATAG+AGG + chr1_3:31761073-31761092 Msa0114560:intron 35.0%
GTGGAAGAAATAGAGGAAAA+TGG + chr1_3:31761080-31761099 Msa0114560:intron 35.0%
AAGAAATAGAGGAAAATGGG+AGG + chr1_3:31761084-31761103 Msa0114560:intron 35.0%
AGAAATAGAGGAAAATGGGA+GGG + chr1_3:31761085-31761104 Msa0114560:intron 35.0%
AATACTAAACCCTCCTCATT+TGG + chr1_3:31761138-31761157 Msa0114560:intron 35.0%
ATACTAAACCCTCCTCATTT+GGG + chr1_3:31761139-31761158 Msa0114560:intron 35.0%
TTTGTGGAGTGGAAAATATG+AGG - chr1_3:31761347-31761366 None:intergenic 35.0%
!! TAAGGCTTTGTTTGTGAGTT+TGG - chr1_3:31761391-31761410 None:intergenic 35.0%
AACTCACAAACAAAGCCTTA+AGG + chr1_3:31761391-31761410 Msa0114560:intron 35.0%
ACAAAGCCTTAAGGATATGA+TGG + chr1_3:31761400-31761419 Msa0114560:intron 35.0%
CAAAGCCTTAAGGATATGAT+GGG + chr1_3:31761401-31761420 Msa0114560:intron 35.0%
ACAAACCCATCATATCCTTA+AGG - chr1_3:31761409-31761428 None:intergenic 35.0%
TGATCACAAGTCTGAAGAAA+AGG + chr1_3:31761583-31761602 Msa0114560:CDS 35.0%
!! AATTTGCTGTGGTTCATCAA+TGG + chr1_3:31761737-31761756 Msa0114560:three_prime_UTR 35.0%
ACCACTTCATTTGTAACACT+TGG + chr1_3:31762064-31762083 Msa0114560:three_prime_UTR 35.0%
CCACTTCATTTGTAACACTT+GGG + chr1_3:31762065-31762084 Msa0114560:three_prime_UTR 35.0%
TGGGAAATACTATTGCGTGA+GGG - chr1_3:31759120-31759139 None:intergenic 40.0%
TTGGGAAATACTATTGCGTG+AGG - chr1_3:31759121-31759140 None:intergenic 40.0%
AAACCCAAATCGAATCTCTC+AGG + chr1_3:31759241-31759260 Msa0114560:CDS 40.0%
! TTAGGTTGTTTGTGGCTTAG+CGG + chr1_3:31759399-31759418 Msa0114560:intron 40.0%
TTTGTGGCTTAGCGGTATTA+CGG + chr1_3:31759407-31759426 Msa0114560:CDS 40.0%
GGCGTGAATAATCTTAACAC+TGG - chr1_3:31759472-31759491 None:intergenic 40.0%
GTGTTAAGATTATTCACGCC+AGG + chr1_3:31759472-31759491 Msa0114560:CDS 40.0%
!! TCACTTTTGCCTGATTGGTT+TGG + chr1_3:31759927-31759946 Msa0114560:intron 40.0%
!!! CACTTTTGCCTGATTGGTTT+GGG + chr1_3:31759928-31759947 Msa0114560:intron 40.0%
!!! ACTTTTGCCTGATTGGTTTG+GGG + chr1_3:31759929-31759948 Msa0114560:intron 40.0%
!!! GGACGAGAATTGGTTTTGAA+TGG + chr1_3:31759950-31759969 Msa0114560:intron 40.0%
! CAGGTTGAGCGAATAAATCA+AGG - chr1_3:31760374-31760393 None:intergenic 40.0%
CTTGATTTATTCGCTCAACC+TGG + chr1_3:31760372-31760391 Msa0114560:intron 40.0%
ATGAAAGTAAGGGAGATGTG+AGG - chr1_3:31760433-31760452 None:intergenic 40.0%
CAAGGGGAAACTTGAATTCA+TGG - chr1_3:31760460-31760479 None:intergenic 40.0%
TCCAATTCTCAGATGGCAAT+TGG + chr1_3:31760497-31760516 Msa0114560:intron 40.0%
TCCAATTGCCATCTGAGAAT+TGG - chr1_3:31760501-31760520 None:intergenic 40.0%
CAGTATTCAATTGAGGAGAC+AGG - chr1_3:31760726-31760745 None:intergenic 40.0%
AAACCGCTATTATCCGTGAT+TGG + chr1_3:31760966-31760985 Msa0114560:intron 40.0%
TTATCCGTGATTGGAAACAC+TGG + chr1_3:31760975-31760994 Msa0114560:intron 40.0%
!! TAAGGCTTTGTTTGCGAGTT+TGG + chr1_3:31761015-31761034 Msa0114560:intron 40.0%
GAAATAGAGGAAAATGGGAG+GGG + chr1_3:31761086-31761105 Msa0114560:intron 40.0%
TACTAAACCCTCCTCATTTG+GGG + chr1_3:31761140-31761159 Msa0114560:intron 40.0%
! TTTAGTTCCCCAAATGAGGA+GGG - chr1_3:31761150-31761169 None:intergenic 40.0%
!!! TTTTAGTTCCCCAAATGAGG+AGG - chr1_3:31761151-31761170 None:intergenic 40.0%
! CTTTGTTTGTGAGTTTGGAG+GGG - chr1_3:31761386-31761405 None:intergenic 40.0%
!! GCTTTGTTTGTGAGTTTGGA+GGG - chr1_3:31761387-31761406 None:intergenic 40.0%
CAGCACAAGAAAAAGTGAAG+TGG - chr1_3:31761456-31761475 None:intergenic 40.0%
ACGCAAGTTCTTTAACTCCA+CGG + chr1_3:31762004-31762023 Msa0114560:three_prime_UTR 40.0%
CCCAAGTGTTACAAATGAAG+TGG - chr1_3:31762068-31762087 None:intergenic 40.0%
TTGCTTGCTTGCTTAGGAGA+GGG - chr1_3:31759206-31759225 None:intergenic 45.0%
ATTGCTTGCTTGCTTAGGAG+AGG - chr1_3:31759207-31759226 None:intergenic 45.0%
GAACACAAACTCCGTACTGT+TGG + chr1_3:31759276-31759295 Msa0114560:CDS 45.0%
TTGTGGCTTAGCGGTATTAC+GGG + chr1_3:31759408-31759427 Msa0114560:CDS 45.0%
CGGGTTCAATTGCGTACAAT+TGG + chr1_3:31759427-31759446 Msa0114560:CDS 45.0%
!! ATTGGTTTGGGGACGAGAAT+TGG + chr1_3:31759940-31759959 Msa0114560:intron 45.0%
CTCAATTGGGGTAGTTGGAA+GGG + chr1_3:31760210-31760229 Msa0114560:intron 45.0%
AATGCTATGCGAGGTATACC+AGG - chr1_3:31760393-31760412 None:intergenic 45.0%
GAAAACTCGAATGCTATGCG+AGG - chr1_3:31760402-31760421 None:intergenic 45.0%
TGAAACAATGCTGACGTGCT+TGG + chr1_3:31760576-31760595 Msa0114560:intron 45.0%
GACACCAGTGTTTCCAATCA+CGG - chr1_3:31760982-31761001 None:intergenic 45.0%
!! GCTTTGTTTGCGAGTTTGGA+GGG + chr1_3:31761019-31761038 Msa0114560:intron 45.0%
! CTTTGTTTGCGAGTTTGGAG+GGG + chr1_3:31761020-31761039 Msa0114560:intron 45.0%
AGGGGAAGGTTTGGGAAAAA+AGG + chr1_3:31761048-31761067 Msa0114560:intron 45.0%
! TGTATTGGAGGAGGGTTTTG+AGG + chr1_3:31761174-31761193 Msa0114560:intron 45.0%
!! GTATTGGAGGAGGGTTTTGA+GGG + chr1_3:31761175-31761194 Msa0114560:intron 45.0%
!!! TATTGGAGGAGGGTTTTGAG+GGG + chr1_3:31761176-31761195 Msa0114560:intron 45.0%
! AAGGAAAAGGCTTTGTGGAG+TGG - chr1_3:31761358-31761377 None:intergenic 45.0%
!! GGCTTTGTTTGTGAGTTTGG+AGG - chr1_3:31761388-31761407 None:intergenic 45.0%
CATGCTCAAGCTCTTACACT+GGG + chr1_3:31761527-31761546 Msa0114560:CDS 45.0%
AGTCTGAAGAAAAGGCTGCA+AGG + chr1_3:31761591-31761610 Msa0114560:CDS 45.0%
GTCTGAAGAAAAGGCTGCAA+GGG + chr1_3:31761592-31761611 Msa0114560:CDS 45.0%
AAAAGGCTGCAAGGGATTCT+AGG + chr1_3:31761600-31761619 Msa0114560:CDS 45.0%
GTATCTGACATCTCACAGCT+TGG + chr1_3:31761677-31761696 Msa0114560:three_prime_UTR 45.0%
GTGGAGTTAAAGAACTTGCG+TGG - chr1_3:31762005-31762024 None:intergenic 45.0%
CGCAAGTTCTTTAACTCCAC+GGG + chr1_3:31762005-31762024 Msa0114560:three_prime_UTR 45.0%
GCAAGTTCTTTAACTCCACG+GGG + chr1_3:31762006-31762025 Msa0114560:three_prime_UTR 45.0%
TCATTTGTAACACTTGGGCG+TGG + chr1_3:31762070-31762089 Msa0114560:three_prime_UTR 45.0%
!! TGCTTCGTTGCTTGATGACG+CGG - chr1_3:31759053-31759072 None:intergenic 50.0%
TTCTCGTCCCCAAACCAATC+AGG - chr1_3:31759939-31759958 None:intergenic 50.0%
GAGTCCTCAATTGGGGTAGT+TGG + chr1_3:31760205-31760224 Msa0114560:intron 50.0%
CCTTCCAACTACCCCAATTG+AGG - chr1_3:31760212-31760231 None:intergenic 50.0%
CCTCAATTGGGGTAGTTGGA+AGG + chr1_3:31760209-31760228 Msa0114560:intron 50.0%
!! GGCTTTGTTTGCGAGTTTGG+AGG + chr1_3:31761018-31761037 Msa0114560:intron 50.0%
TTTGCGAGTTTGGAGGGGAA+GGG + chr1_3:31761025-31761044 Msa0114560:intron 50.0%
ATAGAGGAAAATGGGAGGGG+AGG + chr1_3:31761089-31761108 Msa0114560:intron 50.0%
TAGAGGAAAATGGGAGGGGA+GGG + chr1_3:31761090-31761109 Msa0114560:intron 50.0%
!!! AGGAGGGTTTTGAGGGGTTT+TGG + chr1_3:31761182-31761201 Msa0114560:intron 50.0%
!!! AGGGTTTTGAGGGGTTTTGG+AGG + chr1_3:31761185-31761204 Msa0114560:intron 50.0%
!!! GGGTTTTGAGGGGTTTTGGA+GGG + chr1_3:31761186-31761205 Msa0114560:intron 50.0%
AGGGGAAGGAAAAGGCTTTG+TGG - chr1_3:31761363-31761382 None:intergenic 50.0%
TTTGTGAGTTTGGAGGGGAG+GGG - chr1_3:31761381-31761400 None:intergenic 50.0%
GTTTGTGAGTTTGGAGGGGA+GGG - chr1_3:31761382-31761401 None:intergenic 50.0%
TGTTTGTGAGTTTGGAGGGG+AGG - chr1_3:31761383-31761402 None:intergenic 50.0%
GCATGCTCAAGCTCTTACAC+TGG + chr1_3:31761526-31761545 Msa0114560:CDS 50.0%
!! CTTACACTGGGAGCATTAGC+TGG + chr1_3:31761539-31761558 Msa0114560:CDS 50.0%
TCTTTAACTCCACGGGGATG+AGG + chr1_3:31762012-31762031 Msa0114560:three_prime_UTR 50.0%
TAAGTTTCACCTCATCCCCG+TGG - chr1_3:31762024-31762043 None:intergenic 50.0%
TGACGCGGCGGAACTACTAT+CGG - chr1_3:31759038-31759057 None:intergenic 55.0%
!! TTCGTTGCTTGATGACGCGG+CGG - chr1_3:31759050-31759069 None:intergenic 55.0%
GCGTCATCAAGCAACGAAGC+AGG + chr1_3:31759052-31759071 Msa0114560:five_prime_UTR 55.0%
GTTTGCGAGTTTGGAGGGGA+AGG + chr1_3:31761024-31761043 Msa0114560:intron 55.0%
AAAATGGGAGGGGAGGGCTT+TGG + chr1_3:31761096-31761115 Msa0114560:intron 55.0%
AAATGGGAGGGGAGGGCTTT+GGG + chr1_3:31761097-31761116 Msa0114560:intron 55.0%
AGCATTAGCTGGAGCTGCAG+TGG + chr1_3:31761550-31761569 Msa0114560:CDS 55.0%
CGAGTTTGGAGGGGAAGGGA+GGG + chr1_3:31761029-31761048 Msa0114560:intron 60.0%
GAGTTTGGAGGGGAAGGGAG+GGG + chr1_3:31761030-31761049 Msa0114560:intron 60.0%
GGGAAGGGAGGGGAAGGTTT+GGG + chr1_3:31761040-31761059 Msa0114560:intron 60.0%
AATGGGAGGGGAGGGCTTTG+GGG + chr1_3:31761098-31761117 Msa0114560:intron 60.0%
TGGAGGGGAGGGGAAGGAAA+AGG - chr1_3:31761371-31761390 None:intergenic 60.0%
TGAGTTTGGAGGGGAGGGGA+AGG - chr1_3:31761377-31761396 None:intergenic 60.0%
GCGAGTTTGGAGGGGAAGGG+AGG + chr1_3:31761028-31761047 Msa0114560:intron 65.0%
TTGGAGGGGAAGGGAGGGGA+AGG + chr1_3:31761034-31761053 Msa0114560:intron 65.0%
GGGGAAGGGAGGGGAAGGTT+TGG + chr1_3:31761039-31761058 Msa0114560:intron 65.0%
! ATGGGAGGGGAGGGCTTTGG+GGG + chr1_3:31761099-31761118 Msa0114560:intron 65.0%
!! TGGGAGGGGAGGGCTTTGGG+GGG + chr1_3:31761100-31761119 Msa0114560:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 31759034 31762111 31759034 ID=Msa0114560;Name=Msa0114560
chr1_3 mRNA 31759034 31762111 31759034 ID=Msa0114560-mRNA-1;Parent=Msa0114560;Name=Msa0114560-mRNA-1;_AED=0.05;_eAED=0.05;_QI=194|1|1|1|1|1|3|483|88
chr1_3 exon 31759034 31759297 31759034 ID=Msa0114560-mRNA-1:exon:10321;Parent=Msa0114560-mRNA-1
chr1_3 exon 31759403 31759493 31759403 ID=Msa0114560-mRNA-1:exon:10322;Parent=Msa0114560-mRNA-1
chr1_3 exon 31761523 31762111 31761523 ID=Msa0114560-mRNA-1:exon:10323;Parent=Msa0114560-mRNA-1
chr1_3 five_prime_UTR 31759034 31759227 31759034 ID=Msa0114560-mRNA-1:five_prime_utr;Parent=Msa0114560-mRNA-1
chr1_3 CDS 31759228 31759297 31759228 ID=Msa0114560-mRNA-1:cds;Parent=Msa0114560-mRNA-1
chr1_3 CDS 31759403 31759493 31759403 ID=Msa0114560-mRNA-1:cds;Parent=Msa0114560-mRNA-1
chr1_3 CDS 31761523 31761628 31761523 ID=Msa0114560-mRNA-1:cds;Parent=Msa0114560-mRNA-1
chr1_3 three_prime_UTR 31761629 31762111 31761629 ID=Msa0114560-mRNA-1:three_prime_utr;Parent=Msa0114560-mRNA-1
Gene Sequence

>Msa0114560

ATGTCGGATGCTAAAACCCAAATCGAATCTCTCAGGAAATGGGTCGTTGAACACAAACTCCGTACTGTTGGTTGTTTGTGGCTTAGCGGTATTACGGGTTCAATTGCGTACAATTGGTCTCGTCCTAACATGAAAACCAGTGTTAAGATTATTCACGCCAGGTTGCATGCTCAAGCTCTTACACTGGGAGCATTAGCTGGAGCTGCAGTGGTAGAATATTATGATCACAAGTCTGAAGAAAAGGCTGCAAGGGATTCTAGGCGATAA
Protein sequence

>Msa0114560

MSDAKTQIESLRKWVVEHKLRTVGCLWLSGITGSIAYNWSRPNMKTSVKIIHARLHAQALTLGALAGAAVVEYYDHKSEEKAARDSRR*