AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0032820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032820 MtrunA17_Chr1g0192231 97.778 90 0 1 1 88 1 90 1.71e-59 176
Msa0032820 MtrunA17_Chr3g0114431 46.575 73 38 1 8 79 1 73 1.57e-18 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032820 AT3G05550.1 83.544 79 13 0 1 79 1 79 8.33e-34 112
Msa0032820 AT5G27760.1 78.205 78 17 0 1 78 1 78 1.18e-31 106
Msa0032820 AT5G27760.2 72.222 54 15 0 1 54 1 54 6.87e-27 94.0

Find 53 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGGTGAAACTTATTATT+TGG 0.155300 1_1:-59943263 Msa0032820:three_prime_UTR
TTTGGGCTTTATATTGTATT+GGG 0.313076 1_1:+59946176 None:intergenic
ATTTCCTGAGAGATTCGATT+TGG 0.327609 1_1:+59946067 None:intergenic
AAATCGAATCTCTCAGGAAA+TGG 0.335949 1_1:-59946065 Msa0032820:CDS
AAAAGGCTGCAAGGGATTCT+AGG 0.341872 1_1:-59943692 Msa0032820:CDS
TTGTGGCTTAGCGGTATTAC+GGG 0.371274 1_1:-59945905 Msa0032820:CDS
TCAGCTAGTATAAAGAATTT+CGG 0.373467 1_1:+59943643 None:intergenic
TTTGTGGCTTAGCGGTATTA+CGG 0.376845 1_1:-59945906 Msa0032820:CDS
ACATTATTGCTTGCTTGCTT+AGG 0.385386 1_1:+59946103 None:intergenic
CGGGTTCAATTGCGTACAAT+TGG 0.387745 1_1:-59945886 Msa0032820:CDS
ATTATTTATTAATTGATAGT+TGG 0.403725 1_1:-59943345 Msa0032820:three_prime_UTR
CATTGAAATATTTACTTGCC+AGG 0.410212 1_1:-59943515 Msa0032820:three_prime_UTR
ACCACTTCATTTGTAACACT+TGG 0.413631 1_1:-59943228 Msa0032820:three_prime_UTR
CTTACACTGGGAGCATTAGC+TGG 0.431990 1_1:-59943753 Msa0032820:CDS
CGCAAGTTCTTTAACTCCGC+GGG 0.435474 1_1:-59943287 Msa0032820:three_prime_UTR
TTTCCTGAGAGATTCGATTT+GGG 0.436962 1_1:+59946068 None:intergenic
TTTCTTGTTGCAAATTGCAT+TGG 0.444422 1_1:-59943483 Msa0032820:three_prime_UTR
TGATCACAAGTCTGAAGAAA+AGG 0.467242 1_1:-59943709 Msa0032820:CDS
TTTCTCGATTAGGTTGTTTG+TGG 0.469318 1_1:-59945922 Msa0032820:intron
AATTTGCTGTGGTTCATCAA+TGG 0.470506 1_1:-59943555 Msa0032820:three_prime_UTR
AATCGAATCTCTCAGGAAAT+GGG 0.471588 1_1:-59946064 Msa0032820:CDS
CAACTATTGATTTATATATG+AGG 0.481886 1_1:-59943386 Msa0032820:three_prime_UTR
AAACCCAAATCGAATCTCTC+AGG 0.502597 1_1:-59946071 Msa0032820:CDS
TCTTTAACTCCGCGGGGATG+AGG 0.508766 1_1:-59943280 Msa0032820:three_prime_UTR
GCATGCTCAAGCTCTTACAC+TGG 0.522809 1_1:-59943766 Msa0032820:CDS
GTCTGAAGAAAAGGCTGCAA+GGG 0.524443 1_1:-59943700 Msa0032820:CDS
GTTCCGCCGCGTCATCAAGC+AGG 0.530700 1_1:-59946260 Msa0032820:five_prime_UTR
TTAGGTTGTTTGTGGCTTAG+CGG 0.532960 1_1:-59945914 Msa0032820:intron
CCACTTCATTTGTAACACTT+GGG 0.535710 1_1:-59943227 Msa0032820:three_prime_UTR
GTGTTAAGATTATTCACGCC+AGG 0.537077 1_1:-59945841 Msa0032820:intron
AACAAGAAATATATTCTCCC+TGG 0.539373 1_1:+59943497 None:intergenic
ATTGAAATATTTACTTGCCA+GGG 0.543373 1_1:-59943514 Msa0032820:three_prime_UTR
GTATCTGACATCTCACAGCT+TGG 0.565513 1_1:-59943615 Msa0032820:three_prime_UTR
TTGGAAATTTACCAACAGTA+CGG 0.567029 1_1:+59946025 None:intergenic
TTGCTTGCTTGCTTAGGAGA+GGG 0.573235 1_1:+59946109 None:intergenic
AGCATTAGCTGGAGCTGCAG+TGG 0.578637 1_1:-59943742 Msa0032820:CDS
CCCAAGTGTTACAAATGAAG+TGG 0.581164 1_1:+59943227 None:intergenic
CATGCTCAAGCTCTTACACT+GGG 0.582325 1_1:-59943765 Msa0032820:CDS
GAACACAAACTCCGTACTGT+TGG 0.585849 1_1:-59946036 Msa0032820:intron
AGTCTGAAGAAAAGGCTGCA+AGG 0.589757 1_1:-59943701 Msa0032820:CDS
TGACGCGGCGGAACTACTAT+CGG 0.597040 1_1:+59946269 None:intergenic
TCATTTGTAACACTTGGGCG+TGG 0.598299 1_1:-59943222 Msa0032820:three_prime_UTR
ATTGCTTGCTTGCTTAGGAG+AGG 0.609965 1_1:+59946108 None:intergenic
TTGGGAAATACTATTGCGTG+AGG 0.611327 1_1:+59946194 None:intergenic
TGTAAACCTGCTTGATGACG+CGG 0.617859 1_1:+59946254 None:intergenic
GGCGTGAATAATCTTAACAC+TGG 0.626108 1_1:+59945844 None:intergenic
AAGCAAGCAAGCAATAATGT+CGG 0.649556 1_1:-59946100 Msa0032820:exon
GCGGAGTTAAAGAACTTGCG+TGG 0.665845 1_1:+59943290 None:intergenic
TGGGAAATACTATTGCGTGA+GGG 0.685241 1_1:+59946195 None:intergenic
ACGCAAGTTCTTTAACTCCG+CGG 0.690496 1_1:-59943288 Msa0032820:three_prime_UTR
GCAAGTTCTTTAACTCCGCG+GGG 0.704878 1_1:-59943286 Msa0032820:three_prime_UTR
AAACCTGCTTGATGACGCGG+CGG 0.729792 1_1:+59946257 None:intergenic
TAAGTTTCACCTCATCCCCG+CGG 0.750667 1_1:+59943271 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTATTATTATTATTT+TGG - chr1_1:59943959-59943978 Msa0032820:intron 0.0%
!!! ATTGAAAAGATATATTTTTT+TGG - chr1_1:59944992-59945011 Msa0032820:intron 10.0%
!!! ATTATTTATTAATTGATAGT+TGG - chr1_1:59946128-59946147 Msa0032820:five_prime_UTR 10.0%
!! ATCATATCATCATAAAATAA+AGG + chr1_1:59943490-59943509 None:intergenic 15.0%
!!! ATTTTATGATGATATGATTT+AGG - chr1_1:59943492-59943511 Msa0032820:three_prime_UTR 15.0%
!! TTTCTATACATATTATTATC+TGG - chr1_1:59943676-59943695 Msa0032820:exon 15.0%
!!! AATGAATTTTATACAGTTTA+GGG + chr1_1:59943843-59943862 None:intergenic 15.0%
!!! TAATGAATTTTATACAGTTT+AGG + chr1_1:59943844-59943863 None:intergenic 15.0%
!!! TCAATGTAGAATTTTTATTT+TGG + chr1_1:59945944-59945963 None:intergenic 15.0%
!!! TTTAATTTGTTTTCTCGATT+AGG - chr1_1:59943541-59943560 Msa0032820:three_prime_UTR 20.0%
!! ATAGTAATAATTAACAAACC+TGG + chr1_1:59943653-59943672 None:intergenic 20.0%
!!! ACAGATTTTCATATACTTTT+GGG - chr1_1:59943901-59943920 Msa0032820:intron 20.0%
!!! TAGAGTAGTTAATTAATTTG+AGG - chr1_1:59944168-59944187 Msa0032820:intron 20.0%
!!! TTCAAGTTAGAATCTATTTT+GGG - chr1_1:59944231-59944250 Msa0032820:intron 20.0%
!!! TTTTTGAGTTATTTTGTATC+AGG - chr1_1:59945682-59945701 Msa0032820:intron 20.0%
!!! TCATTTTTGCTCATTTTAAA+AGG - chr1_1:59946031-59946050 Msa0032820:intron 20.0%
!!! CAACTATTGATTTATATATG+AGG - chr1_1:59946087-59946106 Msa0032820:CDS 20.0%
!! TTTGGGCTTTATATTGTATT+GGG + chr1_1:59943300-59943319 None:intergenic 25.0%
!!! TTTTGGGCTTTATATTGTAT+TGG + chr1_1:59943301-59943320 None:intergenic 25.0%
!! AAAAGAAGAAAAGTTTTACG+AGG + chr1_1:59943339-59943358 None:intergenic 25.0%
! AGGAAATTGATTGATTGATT+TGG + chr1_1:59943470-59943489 None:intergenic 25.0%
!! GACAGATTTTCATATACTTT+TGG - chr1_1:59943900-59943919 Msa0032820:intron 25.0%
!!! CTTCAAGTTAGAATCTATTT+TGG - chr1_1:59944230-59944249 Msa0032820:intron 25.0%
!! TCAAGTTAGAATCTATTTTG+GGG - chr1_1:59944232-59944251 Msa0032820:intron 25.0%
!! AACACAATTTTTCTATGACT+TGG + chr1_1:59944469-59944488 None:intergenic 25.0%
! AATAAAAGGATACACTTCAA+GGG + chr1_1:59944666-59944685 None:intergenic 25.0%
! AAATAAAAGGATACACTTCA+AGG + chr1_1:59944667-59944686 None:intergenic 25.0%
! ATCTGAGAATTGGAAATAAA+AGG + chr1_1:59944680-59944699 None:intergenic 25.0%
!!! TTTTATTTCCAATTCTCAGA+TGG - chr1_1:59944679-59944698 Msa0032820:intron 25.0%
!! TTTCTTTTTCTTATTGTCCT+TGG - chr1_1:59944824-59944843 Msa0032820:intron 25.0%
! GAATTATCAGTATTCAATTG+AGG + chr1_1:59944922-59944941 None:intergenic 25.0%
! AAGTTGCATTACTAATTAGA+TGG - chr1_1:59945017-59945036 Msa0032820:intron 25.0%
! AGTTGCATTACTAATTAGAT+GGG - chr1_1:59945018-59945037 Msa0032820:intron 25.0%
!! TATACAGTGTTGTTAAACAT+CGG - chr1_1:59945069-59945088 Msa0032820:intron 25.0%
! GAACTAAAAAATTGTATTGG+AGG - chr1_1:59945351-59945370 Msa0032820:intron 25.0%
! TAAAAAATTGTATTGGAGGA+GGG - chr1_1:59945355-59945374 Msa0032820:intron 25.0%
! TCAGCTAGTATAAAGAATTT+CGG + chr1_1:59945833-59945852 None:intergenic 25.0%
! ATTGAAATATTTACTTGCCA+GGG - chr1_1:59945959-59945978 Msa0032820:intron 25.0%
! ATGAGGTGAAACTTATTATT+TGG - chr1_1:59946210-59946229 Msa0032820:five_prime_UTR 25.0%
! GTAAGAGACTCAAGATTTTT+GGG + chr1_1:59943317-59943336 None:intergenic 30.0%
TTGGAAATTTACCAACAGTA+CGG + chr1_1:59943451-59943470 None:intergenic 30.0%
!!! TTAACACTGGTTTTCATGTT+AGG + chr1_1:59943619-59943638 None:intergenic 30.0%
! TGTTTGATTATTTTCGTTGC+AGG - chr1_1:59943763-59943782 Msa0032820:CDS 30.0%
!! AGATTGTGAAGTTTTGATGA+GGG - chr1_1:59944009-59944028 Msa0032820:intron 30.0%
! TTTTGATGAGGGTTACTATA+GGG - chr1_1:59944020-59944039 Msa0032820:intron 30.0%
TTTAATGAAGCAAGTCATCA+TGG + chr1_1:59944059-59944078 None:intergenic 30.0%
AAAGTGAAAACTACAGCAAA+AGG + chr1_1:59944083-59944102 None:intergenic 30.0%
!!! AGAATTGGTTTTGAATGGAT+TGG - chr1_1:59944124-59944143 Msa0032820:intron 30.0%
!!! TTCTTTTTAGAGTCCTCAAT+TGG - chr1_1:59944363-59944382 Msa0032820:intron 30.0%
!!! TCTTTTTAGAGTCCTCAATT+GGG - chr1_1:59944364-59944383 Msa0032820:intron 30.0%
! GGGTAAGAAATTCTTCTTTT+TGG - chr1_1:59944397-59944416 Msa0032820:intron 30.0%
TCATGGCAAAATGAAAGTAA+GGG + chr1_1:59944632-59944651 None:intergenic 30.0%
TTCATGGCAAAATGAAAGTA+AGG + chr1_1:59944633-59944652 None:intergenic 30.0%
ATAAAAGGATACACTTCAAG+GGG + chr1_1:59944665-59944684 None:intergenic 30.0%
! GCAATTGATGTTCATTTTCA+CGG - chr1_1:59944787-59944806 Msa0032820:intron 30.0%
! CAATTGATGTTCATTTTCAC+GGG - chr1_1:59944788-59944807 Msa0032820:intron 30.0%
TTTCATATAACAAAGAGCCA+AGG + chr1_1:59944844-59944863 None:intergenic 30.0%
ATAAACACCGATCAATTGTT+TGG - chr1_1:59944864-59944883 Msa0032820:intron 30.0%
ATAATCGCCAAACAATTGAT+CGG + chr1_1:59944874-59944893 None:intergenic 30.0%
ATGACAACACTTAATCTCTT+TGG + chr1_1:59944974-59944993 None:intergenic 30.0%
! GTGTCATATTTGAAACACTA+AGG - chr1_1:59945186-59945205 Msa0032820:intron 30.0%
TGGAAGAAATAGAGGAAAAT+GGG - chr1_1:59945270-59945289 Msa0032820:intron 30.0%
!!! ATTTTTTAGTTCCCCAAATG+AGG + chr1_1:59945343-59945362 None:intergenic 30.0%
GGGGAACTAAAAAATTGTAT+TGG - chr1_1:59945348-59945367 Msa0032820:intron 30.0%
CTAAAAAATTGTATTGGAGG+AGG - chr1_1:59945354-59945373 Msa0032820:intron 30.0%
AAAAAATTGTATTGGAGGAG+GGG - chr1_1:59945356-59945375 Msa0032820:intron 30.0%
GTGGAAAATATGAGGAAAAA+TGG + chr1_1:59945521-59945540 None:intergenic 30.0%
AAAGTGAAGTGGAAAATATG+AGG + chr1_1:59945626-59945645 None:intergenic 30.0%
!!! TTTTGCAGTATAATTTGCTG+TGG - chr1_1:59945907-59945926 Msa0032820:CDS 30.0%
CATTGAAATATTTACTTGCC+AGG - chr1_1:59945958-59945977 Msa0032820:intron 30.0%
AACAAGAAATATATTCTCCC+TGG + chr1_1:59945979-59945998 None:intergenic 30.0%
TTTCTTGTTGCAAATTGCAT+TGG - chr1_1:59945990-59946009 Msa0032820:intron 30.0%
! GGTAAGAGACTCAAGATTTT+TGG + chr1_1:59943318-59943337 None:intergenic 35.0%
ACATTATTGCTTGCTTGCTT+AGG + chr1_1:59943373-59943392 None:intergenic 35.0%
AAGCAAGCAAGCAATAATGT+CGG - chr1_1:59943373-59943392 Msa0032820:three_prime_UTR 35.0%
TTTCCTGAGAGATTCGATTT+GGG + chr1_1:59943408-59943427 None:intergenic 35.0%
ATTTCCTGAGAGATTCGATT+TGG + chr1_1:59943409-59943428 None:intergenic 35.0%
AAATCGAATCTCTCAGGAAA+TGG - chr1_1:59943408-59943427 Msa0032820:three_prime_UTR 35.0%
AATCGAATCTCTCAGGAAAT+GGG - chr1_1:59943409-59943428 Msa0032820:three_prime_UTR 35.0%
! TTTCTCGATTAGGTTGTTTG+TGG - chr1_1:59943551-59943570 Msa0032820:three_prime_UTR 35.0%
AATTTCGTTGCATTGCATGA+AGG - chr1_1:59943739-59943758 Msa0032820:CDS 35.0%
ATTTCGTTGCATTGCATGAA+GGG - chr1_1:59943740-59943759 Msa0032820:CDS 35.0%
!! GAGATTGTGAAGTTTTGATG+AGG - chr1_1:59944008-59944027 Msa0032820:intron 35.0%
!! GTTTTGATGAGGGTTACTAT+AGG - chr1_1:59944019-59944038 Msa0032820:intron 35.0%
!! AGTTTTCACTTTTGCCTGAT+TGG - chr1_1:59944091-59944110 Msa0032820:intron 35.0%
!!! GGTTTTGAATGGATTGGTTT+TGG - chr1_1:59944130-59944149 Msa0032820:intron 35.0%
!!! CTTTTTAGAGTCCTCAATTG+GGG - chr1_1:59944365-59944384 Msa0032820:intron 35.0%
!!! TGCGTTGTAGAAGCTTTTTT+TGG - chr1_1:59944427-59944446 Msa0032820:intron 35.0%
GAAAAATTGTGTTTCGCAAC+AGG - chr1_1:59944476-59944495 Msa0032820:intron 35.0%
! TGTTGTTTGATTCTAGTGCA+TGG - chr1_1:59944719-59944738 Msa0032820:intron 35.0%
CATTACTAATTAGATGGGCA+TGG - chr1_1:59945023-59945042 Msa0032820:intron 35.0%
TAATTAGATGGGCATGGAAA+TGG - chr1_1:59945029-59945048 Msa0032820:intron 35.0%
TGGAAATGGAGTGTAAAACA+AGG - chr1_1:59945043-59945062 Msa0032820:intron 35.0%
GTAAACTCTGCTACATAACA+CGG + chr1_1:59945133-59945152 None:intergenic 35.0%
TTTCCAATCACGGATAATAG+CGG + chr1_1:59945161-59945180 None:intergenic 35.0%
GGAAAAAAGGAATGAACAAG+TGG - chr1_1:59945250-59945269 Msa0032820:intron 35.0%
TGAACAAGTGGAAGAAATAG+AGG - chr1_1:59945262-59945281 Msa0032820:intron 35.0%
GTGGAAGAAATAGAGGAAAA+TGG - chr1_1:59945269-59945288 Msa0032820:intron 35.0%
AAGAAATAGAGGAAAATGGG+AGG - chr1_1:59945273-59945292 Msa0032820:intron 35.0%
AGAAATAGAGGAAAATGGGA+GGG - chr1_1:59945274-59945293 Msa0032820:intron 35.0%
AATACTAAACCCTCCTCATT+TGG - chr1_1:59945327-59945346 Msa0032820:intron 35.0%
ATACTAAACCCTCCTCATTT+GGG - chr1_1:59945328-59945347 Msa0032820:intron 35.0%
AAAAATTGTATTGGAGGAGG+GGG - chr1_1:59945357-59945376 Msa0032820:intron 35.0%
TTTGTGGAGTGGAAAATATG+AGG + chr1_1:59945529-59945548 None:intergenic 35.0%
!! TAAGGCTTTGTTTGTGAGTT+TGG + chr1_1:59945572-59945591 None:intergenic 35.0%
AACTCACAAACAAAGCCTTA+AGG - chr1_1:59945572-59945591 Msa0032820:intron 35.0%
ACAAAGCCTTAAGGATATGA+TGG - chr1_1:59945581-59945600 Msa0032820:intron 35.0%
CAAAGCCTTAAGGATATGAT+GGG - chr1_1:59945582-59945601 Msa0032820:intron 35.0%
ACAAACCCATCATATCCTTA+AGG + chr1_1:59945590-59945609 None:intergenic 35.0%
TGATCACAAGTCTGAAGAAA+AGG - chr1_1:59945764-59945783 Msa0032820:intron 35.0%
!! AATTTGCTGTGGTTCATCAA+TGG - chr1_1:59945918-59945937 Msa0032820:intron 35.0%
ACCACTTCATTTGTAACACT+TGG - chr1_1:59946245-59946264 Msa0032820:five_prime_UTR 35.0%
CCACTTCATTTGTAACACTT+GGG - chr1_1:59946246-59946265 Msa0032820:five_prime_UTR 35.0%
TGGGAAATACTATTGCGTGA+GGG + chr1_1:59943281-59943300 None:intergenic 40.0%
TTGGGAAATACTATTGCGTG+AGG + chr1_1:59943282-59943301 None:intergenic 40.0%
AAACCCAAATCGAATCTCTC+AGG - chr1_1:59943402-59943421 Msa0032820:three_prime_UTR 40.0%
! TTAGGTTGTTTGTGGCTTAG+CGG - chr1_1:59943559-59943578 Msa0032820:three_prime_UTR 40.0%
TTTGTGGCTTAGCGGTATTA+CGG - chr1_1:59943567-59943586 Msa0032820:three_prime_UTR 40.0%
GGCGTGAATAATCTTAACAC+TGG + chr1_1:59943632-59943651 None:intergenic 40.0%
GTGTTAAGATTATTCACGCC+AGG - chr1_1:59943632-59943651 Msa0032820:three_prime_UTR 40.0%
!! ACTTTTGCCTGATTGGTTCT+GGG - chr1_1:59944098-59944117 Msa0032820:intron 40.0%
!!! GGACGAGAATTGGTTTTGAA+TGG - chr1_1:59944119-59944138 Msa0032820:intron 40.0%
! CAGGTTGAGCGAATAAATCA+AGG + chr1_1:59944541-59944560 None:intergenic 40.0%
CTTGATTTATTCGCTCAACC+TGG - chr1_1:59944539-59944558 Msa0032820:intron 40.0%
GCATGGCAAAACGAAAGTAA+GGG + chr1_1:59944579-59944598 None:intergenic 40.0%
TGCATGGCAAAACGAAAGTA+AGG + chr1_1:59944580-59944599 None:intergenic 40.0%
CAAGAGAAAACTCGAATGCA+TGG + chr1_1:59944596-59944615 None:intergenic 40.0%
ATGAAAGTAAGGGAGATGTG+AGG + chr1_1:59944622-59944641 None:intergenic 40.0%
CAAGGGGAAACTTGAATTCA+TGG + chr1_1:59944649-59944668 None:intergenic 40.0%
TCCAATTCTCAGATGGCAAT+TGG - chr1_1:59944686-59944705 Msa0032820:intron 40.0%
TCCAATTGCCATCTGAGAAT+TGG + chr1_1:59944690-59944709 None:intergenic 40.0%
CAGTATTCAATTGAGGAGAC+AGG + chr1_1:59944915-59944934 None:intergenic 40.0%
AAACCGCTATTATCCGTGAT+TGG - chr1_1:59945155-59945174 Msa0032820:intron 40.0%
TTATCCGTGATTGGAAACAC+TGG - chr1_1:59945164-59945183 Msa0032820:intron 40.0%
!! TAAGGCTTTGTTTGCGAGTT+TGG - chr1_1:59945204-59945223 Msa0032820:intron 40.0%
GAAATAGAGGAAAATGGGAG+GGG - chr1_1:59945275-59945294 Msa0032820:intron 40.0%
TACTAAACCCTCCTCATTTG+GGG - chr1_1:59945329-59945348 Msa0032820:intron 40.0%
! TTTAGTTCCCCAAATGAGGA+GGG + chr1_1:59945339-59945358 None:intergenic 40.0%
!!! TTTTAGTTCCCCAAATGAGG+AGG + chr1_1:59945340-59945359 None:intergenic 40.0%
AAAATTGTATTGGAGGAGGG+GGG - chr1_1:59945358-59945377 Msa0032820:intron 40.0%
! CTTTGTTTGTGAGTTTGGAG+GGG + chr1_1:59945567-59945586 None:intergenic 40.0%
!! GCTTTGTTTGTGAGTTTGGA+GGG + chr1_1:59945568-59945587 None:intergenic 40.0%
CAGCACAAGAAAAAGTGAAG+TGG + chr1_1:59945637-59945656 None:intergenic 40.0%
CCCAAGTGTTACAAATGAAG+TGG + chr1_1:59946249-59946268 None:intergenic 40.0%
!! TGTAAACCTGCTTGATGACG+CGG + chr1_1:59943222-59943241 None:intergenic 45.0%
TTGCTTGCTTGCTTAGGAGA+GGG + chr1_1:59943367-59943386 None:intergenic 45.0%
ATTGCTTGCTTGCTTAGGAG+AGG + chr1_1:59943368-59943387 None:intergenic 45.0%
GAACACAAACTCCGTACTGT+TGG - chr1_1:59943437-59943456 Msa0032820:three_prime_UTR 45.0%
TTGTGGCTTAGCGGTATTAC+GGG - chr1_1:59943568-59943587 Msa0032820:three_prime_UTR 45.0%
CGGGTTCAATTGCGTACAAT+TGG - chr1_1:59943587-59943606 Msa0032820:three_prime_UTR 45.0%
!! CACTTTTGCCTGATTGGTTC+TGG - chr1_1:59944097-59944116 Msa0032820:intron 45.0%
!! ATTGGTTCTGGGACGAGAAT+TGG - chr1_1:59944109-59944128 Msa0032820:intron 45.0%
CTCAATTGGGGTAGTTGGAA+GGG - chr1_1:59944377-59944396 Msa0032820:intron 45.0%
TGAAACAATGCTGACGTGCT+TGG - chr1_1:59944765-59944784 Msa0032820:intron 45.0%
GACACCAGTGTTTCCAATCA+CGG + chr1_1:59945171-59945190 None:intergenic 45.0%
!! GCTTTGTTTGCGAGTTTGGA+GGG - chr1_1:59945208-59945227 Msa0032820:intron 45.0%
! CTTTGTTTGCGAGTTTGGAG+GGG - chr1_1:59945209-59945228 Msa0032820:intron 45.0%
AGGGGAAGGTTTGGGAAAAA+AGG - chr1_1:59945237-59945256 Msa0032820:intron 45.0%
!! GGCTTTGTTTGTGAGTTTGG+AGG + chr1_1:59945569-59945588 None:intergenic 45.0%
CATGCTCAAGCTCTTACACT+GGG - chr1_1:59945708-59945727 Msa0032820:intron 45.0%
AGTCTGAAGAAAAGGCTGCA+AGG - chr1_1:59945772-59945791 Msa0032820:intron 45.0%
GTCTGAAGAAAAGGCTGCAA+GGG - chr1_1:59945773-59945792 Msa0032820:intron 45.0%
AAAAGGCTGCAAGGGATTCT+AGG - chr1_1:59945781-59945800 Msa0032820:intron 45.0%
GTATCTGACATCTCACAGCT+TGG - chr1_1:59945858-59945877 Msa0032820:CDS 45.0%
ACGCAAGTTCTTTAACTCCG+CGG - chr1_1:59946185-59946204 Msa0032820:five_prime_UTR 45.0%
TCATTTGTAACACTTGGGCG+TGG - chr1_1:59946251-59946270 Msa0032820:five_prime_UTR 45.0%
TTCTCGTCCCAGAACCAATC+AGG + chr1_1:59944108-59944127 None:intergenic 50.0%
GAGTCCTCAATTGGGGTAGT+TGG - chr1_1:59944372-59944391 Msa0032820:intron 50.0%
CCTTCCAACTACCCCAATTG+AGG + chr1_1:59944379-59944398 None:intergenic 50.0%
CCTCAATTGGGGTAGTTGGA+AGG - chr1_1:59944376-59944395 Msa0032820:intron 50.0%
ACGAAAGTAAGGGAGATGCG+AGG + chr1_1:59944569-59944588 None:intergenic 50.0%
!! GGCTTTGTTTGCGAGTTTGG+AGG - chr1_1:59945207-59945226 Msa0032820:intron 50.0%
TTTGCGAGTTTGGAGGGGAA+GGG - chr1_1:59945214-59945233 Msa0032820:intron 50.0%
ATAGAGGAAAATGGGAGGGG+AGG - chr1_1:59945278-59945297 Msa0032820:intron 50.0%
TAGAGGAAAATGGGAGGGGA+GGG - chr1_1:59945279-59945298 Msa0032820:intron 50.0%
! GAAGGAAAGGCTTTGTGGAG+TGG + chr1_1:59945540-59945559 None:intergenic 50.0%
TTTGTGAGTTTGGAGGGGAG+GGG + chr1_1:59945562-59945581 None:intergenic 50.0%
GTTTGTGAGTTTGGAGGGGA+GGG + chr1_1:59945563-59945582 None:intergenic 50.0%
TGTTTGTGAGTTTGGAGGGG+AGG + chr1_1:59945564-59945583 None:intergenic 50.0%
GCATGCTCAAGCTCTTACAC+TGG - chr1_1:59945707-59945726 Msa0032820:intron 50.0%
!! CTTACACTGGGAGCATTAGC+TGG - chr1_1:59945720-59945739 Msa0032820:intron 50.0%
GCGGAGTTAAAGAACTTGCG+TGG + chr1_1:59946186-59946205 None:intergenic 50.0%
CGCAAGTTCTTTAACTCCGC+GGG - chr1_1:59946186-59946205 Msa0032820:five_prime_UTR 50.0%
GCAAGTTCTTTAACTCCGCG+GGG - chr1_1:59946187-59946206 Msa0032820:five_prime_UTR 50.0%
TAAGTTTCACCTCATCCCCG+CGG + chr1_1:59946205-59946224 None:intergenic 50.0%
TGACGCGGCGGAACTACTAT+CGG + chr1_1:59943207-59943226 None:intergenic 55.0%
!! AAACCTGCTTGATGACGCGG+CGG + chr1_1:59943219-59943238 None:intergenic 55.0%
AGGGAGATGCGAGGTATACC+AGG + chr1_1:59944560-59944579 None:intergenic 55.0%
GTTTGCGAGTTTGGAGGGGA+AGG - chr1_1:59945213-59945232 Msa0032820:intron 55.0%
AAAATGGGAGGGGAGGGCTT+TGG - chr1_1:59945285-59945304 Msa0032820:intron 55.0%
AAATGGGAGGGGAGGGCTTT+GGG - chr1_1:59945286-59945305 Msa0032820:intron 55.0%
! GTATTGGAGGAGGGGGGTTT+TGG - chr1_1:59945364-59945383 Msa0032820:intron 55.0%
GAGGGGAAGGAAAGGCTTTG+TGG + chr1_1:59945545-59945564 None:intergenic 55.0%
AGCATTAGCTGGAGCTGCAG+TGG - chr1_1:59945731-59945750 Msa0032820:intron 55.0%
TCTTTAACTCCGCGGGGATG+AGG - chr1_1:59946193-59946212 Msa0032820:five_prime_UTR 55.0%
CGAGTTTGGAGGGGAAGGGA+GGG - chr1_1:59945218-59945237 Msa0032820:intron 60.0%
GAGTTTGGAGGGGAAGGGAG+GGG - chr1_1:59945219-59945238 Msa0032820:intron 60.0%
GGGAAGGGAGGGGAAGGTTT+GGG - chr1_1:59945229-59945248 Msa0032820:intron 60.0%
AATGGGAGGGGAGGGCTTTG+GGG - chr1_1:59945287-59945306 Msa0032820:intron 60.0%
!! TTGGAGGAGGGGGGTTTTGG+AGG - chr1_1:59945367-59945386 Msa0032820:intron 60.0%
!!! TGGAGGAGGGGGGTTTTGGA+GGG - chr1_1:59945368-59945387 Msa0032820:intron 60.0%
TTGGAGGGGAGGGGAAGGAA+AGG + chr1_1:59945553-59945572 None:intergenic 60.0%
TGAGTTTGGAGGGGAGGGGA+AGG + chr1_1:59945558-59945577 None:intergenic 60.0%
GTTCCGCCGCGTCATCAAGC+AGG - chr1_1:59943213-59943232 Msa0032820:three_prime_UTR 65.0%
GCGAGTTTGGAGGGGAAGGG+AGG - chr1_1:59945217-59945236 Msa0032820:intron 65.0%
TTGGAGGGGAAGGGAGGGGA+AGG - chr1_1:59945223-59945242 Msa0032820:intron 65.0%
GGGGAAGGGAGGGGAAGGTT+TGG - chr1_1:59945228-59945247 Msa0032820:intron 65.0%
! ATGGGAGGGGAGGGCTTTGG+GGG - chr1_1:59945288-59945307 Msa0032820:intron 65.0%
!! TGGGAGGGGAGGGCTTTGGG+GGG - chr1_1:59945289-59945308 Msa0032820:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 59943203 59946292 59943203 ID=Msa0032820;Name=Msa0032820
chr1_1 mRNA 59943203 59946292 59943203 ID=Msa0032820-mRNA-1;Parent=Msa0032820;Name=Msa0032820-mRNA-1;_AED=0.01;_eAED=0.01;_QI=186|1|1|1|1|1|3|483|88
chr1_1 exon 59943203 59943791 59943203 ID=Msa0032820-mRNA-1:exon:18666;Parent=Msa0032820-mRNA-1
chr1_1 exon 59945842 59945932 59945842 ID=Msa0032820-mRNA-1:exon:18665;Parent=Msa0032820-mRNA-1
chr1_1 exon 59946037 59946292 59946037 ID=Msa0032820-mRNA-1:exon:18664;Parent=Msa0032820-mRNA-1
chr1_1 five_prime_UTR 59946107 59946292 59946107 ID=Msa0032820-mRNA-1:five_prime_utr;Parent=Msa0032820-mRNA-1
chr1_1 CDS 59946037 59946106 59946037 ID=Msa0032820-mRNA-1:cds;Parent=Msa0032820-mRNA-1
chr1_1 CDS 59945842 59945932 59945842 ID=Msa0032820-mRNA-1:cds;Parent=Msa0032820-mRNA-1
chr1_1 CDS 59943686 59943791 59943686 ID=Msa0032820-mRNA-1:cds;Parent=Msa0032820-mRNA-1
chr1_1 three_prime_UTR 59943203 59943685 59943203 ID=Msa0032820-mRNA-1:three_prime_utr;Parent=Msa0032820-mRNA-1
Gene Sequence

>Msa0032820

ATGTCGGATGCTAAAACCCAAATCGAATCTCTCAGGAAATGGGTCGTTGAACACAAACTCCGTACTGTTGGTTGTTTGTGGCTTAGCGGTATTACGGGTTCAATTGCGTACAATTGGTCTCGTCCTAACATGAAAACCAGTGTTAAGATTATTCACGCCAGGTTGCATGCTCAAGCTCTTACACTGGGAGCATTAGCTGGAGCTGCAGTGGTAGAATATTATGATCACAAGTCTGAAGAAAAGGCTGCAAGGGATTCTAGGCGATAA
Protein sequence

>Msa0032820

MSDAKTQIESLRKWVVEHKLRTVGCLWLSGITGSIAYNWSRPNMKTSVKIIHARLHAQALTLGALAGAAVVEYYDHKSEEKAARDSRR*