AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0230530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230530 MtrunA17_Chr1g0192231 97.778 90 0 1 1 88 1 90 1.71e-59 176
Msa0230530 MtrunA17_Chr3g0114431 46.575 73 38 1 8 79 1 73 1.57e-18 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230530 AT3G05550.1 83.544 79 13 0 1 79 1 79 8.33e-34 112
Msa0230530 AT5G27760.1 78.205 78 17 0 1 78 1 78 1.18e-31 106
Msa0230530 AT5G27760.2 72.222 54 15 0 1 54 1 54 6.87e-27 94.0

Find 52 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGGTGAAACTTATTATT+TGG 0.155300 2_2:-2714546 Msa0230530:three_prime_UTR
TTTGGGCTTTATATTGTATT+GGG 0.313076 2_2:+2717459 None:intergenic
ATTTCCTGAGAGATTCGATT+TGG 0.327609 2_2:+2717354 None:intergenic
AAATCGAATCTCTCAGGAAA+TGG 0.335949 2_2:-2717352 Msa0230530:CDS
AAAAGGCTGCAAGGGATTCT+AGG 0.341872 2_2:-2714975 Msa0230530:CDS
TTGTGGCTTAGCGGTATTAC+GGG 0.371274 2_2:-2717194 Msa0230530:CDS
TCAGCTAGTATAAAGAATTT+CGG 0.373467 2_2:+2714926 None:intergenic
TTTGTGGCTTAGCGGTATTA+CGG 0.376845 2_2:-2717195 Msa0230530:CDS
CGGGTTCAATTGCGTACAAT+TGG 0.387745 2_2:-2717175 Msa0230530:CDS
ATTATTTATTAATTGATAGT+TGG 0.403725 2_2:-2714628 Msa0230530:three_prime_UTR
CATTGAAATATTTACTTGCC+AGG 0.410212 2_2:-2714798 Msa0230530:three_prime_UTR
ACCACTTCATTTGTAACACT+TGG 0.413631 2_2:-2714511 Msa0230530:three_prime_UTR
CGCAAGTTCTTTAACTCCAC+GGG 0.423820 2_2:-2714570 Msa0230530:three_prime_UTR
CTTACACTGGGAGCATTAGC+TGG 0.431990 2_2:-2715036 Msa0230530:CDS
TTTCCTGAGAGATTCGATTT+GGG 0.436962 2_2:+2717355 None:intergenic
TCCGACATTATTGCTTGCTT+AGG 0.442053 2_2:+2717386 None:intergenic
TTTCTTGTTGCAAATTGCAT+TGG 0.444422 2_2:-2714766 Msa0230530:three_prime_UTR
TGATCACAAGTCTGAAGAAA+AGG 0.467242 2_2:-2714992 Msa0230530:CDS
TTTCTCGATTAGGTTGTTTG+TGG 0.469318 2_2:-2717211 Msa0230530:intron
AATTTGCTGTGGTTCATCAA+TGG 0.470506 2_2:-2714838 Msa0230530:three_prime_UTR
AATCGAATCTCTCAGGAAAT+GGG 0.471588 2_2:-2717351 Msa0230530:CDS
CAACTATTGATTTATATATG+AGG 0.481886 2_2:-2714669 Msa0230530:three_prime_UTR
AAACCCAAATCGAATCTCTC+AGG 0.502597 2_2:-2717358 Msa0230530:CDS
TCTTTAACTCCACGGGGATG+AGG 0.522631 2_2:-2714563 Msa0230530:three_prime_UTR
GCATGCTCAAGCTCTTACAC+TGG 0.522809 2_2:-2715049 Msa0230530:CDS
GTCTGAAGAAAAGGCTGCAA+GGG 0.524443 2_2:-2714983 Msa0230530:CDS
TTAGGTTGTTTGTGGCTTAG+CGG 0.532960 2_2:-2717203 Msa0230530:intron
CCACTTCATTTGTAACACTT+GGG 0.535710 2_2:-2714510 Msa0230530:three_prime_UTR
GTGTTAAGATTATTCACGCC+AGG 0.537077 2_2:-2717130 Msa0230530:intron
AACAAGAAATATATTCTCCC+TGG 0.539373 2_2:+2714780 None:intergenic
ATTGAAATATTTACTTGCCA+GGG 0.543373 2_2:-2714797 Msa0230530:three_prime_UTR
GCGTCATCAAGCAACGAAGC+AGG 0.552051 2_2:-2717543 Msa0230530:five_prime_UTR
GTATCTGACATCTCACAGCT+TGG 0.565513 2_2:-2714898 Msa0230530:three_prime_UTR
TTGGAAATTTACCAACAGTA+CGG 0.567029 2_2:+2717312 None:intergenic
CATTATTGCTTGCTTAGGAG+AGG 0.576216 2_2:+2717391 None:intergenic
AGCATTAGCTGGAGCTGCAG+TGG 0.578637 2_2:-2715025 Msa0230530:CDS
ATTATTGCTTGCTTAGGAGA+GGG 0.579597 2_2:+2717392 None:intergenic
CCCAAGTGTTACAAATGAAG+TGG 0.581164 2_2:+2714510 None:intergenic
ATGACGCGGCGAACTACTAT+CGG 0.581473 2_2:+2717559 None:intergenic
CATGCTCAAGCTCTTACACT+GGG 0.582325 2_2:-2715048 Msa0230530:CDS
GAACACAAACTCCGTACTGT+TGG 0.585849 2_2:-2717323 Msa0230530:intron
AGTCTGAAGAAAAGGCTGCA+AGG 0.589757 2_2:-2714984 Msa0230530:CDS
ACGCAAGTTCTTTAACTCCA+CGG 0.590810 2_2:-2714571 Msa0230530:three_prime_UTR
TGCTTCGTTGCTTGATGACG+CGG 0.593098 2_2:+2717545 None:intergenic
TCATTTGTAACACTTGGGCG+TGG 0.598299 2_2:-2714505 Msa0230530:three_prime_UTR
TTGGGAAATACTATTGCGTG+AGG 0.611327 2_2:+2717477 None:intergenic
TCCTAAGCAAGCAATAATGT+CGG 0.625902 2_2:-2717387 Msa0230530:exon
GGCGTGAATAATCTTAACAC+TGG 0.626108 2_2:+2717133 None:intergenic
GTGGAGTTAAAGAACTTGCG+TGG 0.662404 2_2:+2714573 None:intergenic
TGGGAAATACTATTGCGTGA+GGG 0.685241 2_2:+2717478 None:intergenic
TAAGTTTCACCTCATCCCCG+TGG 0.693295 2_2:+2714554 None:intergenic
GCAAGTTCTTTAACTCCACG+GGG 0.741920 2_2:-2714569 Msa0230530:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTATTATTATTATTT+TGG - chr2_2:2715246-2715265 Msa0230530:intron 0.0%
!! TAAATCATATCATAAAATAA+AGG + chr2_2:2714776-2714795 None:intergenic 10.0%
!!! TTTATTTTATGATATGATTT+AGG - chr2_2:2714775-2714794 Msa0230530:three_prime_UTR 10.0%
!!! ATTGAAAAGATATATTTTTT+TGG - chr2_2:2716281-2716300 Msa0230530:intron 10.0%
!!! ATTATTTATTAATTGATAGT+TGG - chr2_2:2717418-2717437 Msa0230530:five_prime_UTR 10.0%
!! TTTCTATACATATTATTATC+TGG - chr2_2:2714960-2714979 Msa0230530:exon 15.0%
!!! AATGAATTTTATACAGTTTA+GGG + chr2_2:2715130-2715149 None:intergenic 15.0%
!!! TAATGAATTTTATACAGTTT+AGG + chr2_2:2715131-2715150 None:intergenic 15.0%
!!! TCAATGTAGAATTTTTATTT+TGG + chr2_2:2717234-2717253 None:intergenic 15.0%
!!! TTTAATTTGTTTTCTCGATT+AGG - chr2_2:2714825-2714844 Msa0230530:three_prime_UTR 20.0%
!! ATAGTAATAATTAACAAACC+TGG + chr2_2:2714937-2714956 None:intergenic 20.0%
!!! TTTAGTTCTAAGATCTTAAT+AGG + chr2_2:2715165-2715184 None:intergenic 20.0%
!!! ACAGATTTTCATATACTTTT+GGG - chr2_2:2715188-2715207 Msa0230530:intron 20.0%
!!! TAGAGTAGTTAATTAATTTG+AGG - chr2_2:2715455-2715474 Msa0230530:intron 20.0%
!!! TTCAAGTTAGAATCTATTTT+GGG - chr2_2:2715518-2715537 Msa0230530:intron 20.0%
!!! TTTTTGAGTTATTTTGTATC+AGG - chr2_2:2716972-2716991 Msa0230530:intron 20.0%
!!! TCATTTTTGCTCATTTTAAA+AGG - chr2_2:2717321-2717340 Msa0230530:intron 20.0%
!!! CAACTATTGATTTATATATG+AGG - chr2_2:2717377-2717396 Msa0230530:exon 20.0%
!! TTTGGGCTTTATATTGTATT+GGG + chr2_2:2714590-2714609 None:intergenic 25.0%
!!! TTTTGGGCTTTATATTGTAT+TGG + chr2_2:2714591-2714610 None:intergenic 25.0%
!! ATAAGAGACTCAAGATTTTT+GGG + chr2_2:2714607-2714626 None:intergenic 25.0%
! AGGAAATTGATTGATTGATT+TGG + chr2_2:2714756-2714775 None:intergenic 25.0%
!! GACAGATTTTCATATACTTT+TGG - chr2_2:2715187-2715206 Msa0230530:intron 25.0%
!!! CTTCAAGTTAGAATCTATTT+TGG - chr2_2:2715517-2715536 Msa0230530:intron 25.0%
!! TCAAGTTAGAATCTATTTTG+GGG - chr2_2:2715519-2715538 Msa0230530:intron 25.0%
!! AACACAATTTTTCTATGACT+TGG + chr2_2:2715758-2715777 None:intergenic 25.0%
! AATAAAAGGATACACTTCAA+GGG + chr2_2:2715955-2715974 None:intergenic 25.0%
! AAATAAAAGGATACACTTCA+AGG + chr2_2:2715956-2715975 None:intergenic 25.0%
! ATCTGAGAATTGGAAATAAA+AGG + chr2_2:2715969-2715988 None:intergenic 25.0%
!!! TTTTATTTCCAATTCTCAGA+TGG - chr2_2:2715968-2715987 Msa0230530:intron 25.0%
!! TTTCTTTTTCTTATTGTCCT+TGG - chr2_2:2716113-2716132 Msa0230530:intron 25.0%
! GAATTATCAGTATTCAATTG+AGG + chr2_2:2716211-2716230 None:intergenic 25.0%
! AAGTTGCATTACTAATTAGA+TGG - chr2_2:2716306-2716325 Msa0230530:intron 25.0%
! AGTTGCATTACTAATTAGAT+GGG - chr2_2:2716307-2716326 Msa0230530:intron 25.0%
!! TATACAGTGTTGTTAAACAT+CGG - chr2_2:2716358-2716377 Msa0230530:intron 25.0%
! GAACTAAAAAATTGTATTGG+AGG - chr2_2:2716630-2716649 Msa0230530:intron 25.0%
! TAAAAAATTGTATTGGAGGA+GGG - chr2_2:2716634-2716653 Msa0230530:intron 25.0%
! TCAGCTAGTATAAAGAATTT+CGG + chr2_2:2717123-2717142 None:intergenic 25.0%
! ATTGAAATATTTACTTGCCA+GGG - chr2_2:2717249-2717268 Msa0230530:intron 25.0%
! ATGAGGTGAAACTTATTATT+TGG - chr2_2:2717500-2717519 Msa0230530:five_prime_UTR 25.0%
! GATAAGAGACTCAAGATTTT+TGG + chr2_2:2714608-2714627 None:intergenic 30.0%
TTGGAAATTTACCAACAGTA+CGG + chr2_2:2714737-2714756 None:intergenic 30.0%
!!! TTAACACTGGTTTTCATGTT+AGG + chr2_2:2714903-2714922 None:intergenic 30.0%
! TGTTTGATTATTTTCGTTGC+AGG - chr2_2:2715047-2715066 Msa0230530:CDS 30.0%
!! AGATTGTGAAGTTTTGATGA+GGG - chr2_2:2715296-2715315 Msa0230530:intron 30.0%
! TTTTGATGAGGGTTACTATA+GGG - chr2_2:2715307-2715326 Msa0230530:intron 30.0%
TTTAATGAAGCAAGTCATCA+TGG + chr2_2:2715346-2715365 None:intergenic 30.0%
AAAGTGAAAACTACAGCAAA+AGG + chr2_2:2715370-2715389 None:intergenic 30.0%
!!! AGAATTGGTTTTGAATGGAT+TGG - chr2_2:2715411-2715430 Msa0230530:intron 30.0%
!!! TTCTTTTTAGAGTCCTCAAT+TGG - chr2_2:2715652-2715671 Msa0230530:intron 30.0%
!!! TCTTTTTAGAGTCCTCAATT+GGG - chr2_2:2715653-2715672 Msa0230530:intron 30.0%
! GGGTAAGAAATTCTTCTTTT+TGG - chr2_2:2715686-2715705 Msa0230530:intron 30.0%
TCATGGCAAAATGAAAGTAA+GGG + chr2_2:2715921-2715940 None:intergenic 30.0%
TTCATGGCAAAATGAAAGTA+AGG + chr2_2:2715922-2715941 None:intergenic 30.0%
ATAAAAGGATACACTTCAAG+GGG + chr2_2:2715954-2715973 None:intergenic 30.0%
! GCAATTGATGTTCATTTTCA+CGG - chr2_2:2716076-2716095 Msa0230530:intron 30.0%
! CAATTGATGTTCATTTTCAC+GGG - chr2_2:2716077-2716096 Msa0230530:intron 30.0%
TTTCATATAACAAAGAGCCA+AGG + chr2_2:2716133-2716152 None:intergenic 30.0%
ATAAACACCGATCAATTGTT+TGG - chr2_2:2716153-2716172 Msa0230530:intron 30.0%
ATAATCGCCAAACAATTGAT+CGG + chr2_2:2716163-2716182 None:intergenic 30.0%
ATGACAACACTTAATCTCTT+TGG + chr2_2:2716263-2716282 None:intergenic 30.0%
! GTGTCATATTTGAAACACTA+AGG - chr2_2:2716475-2716494 Msa0230530:intron 30.0%
TGGAAGAAATAGAGGAAAAT+GGG - chr2_2:2716549-2716568 Msa0230530:intron 30.0%
!!! ATTTTTTAGTTCCCCAAATG+AGG + chr2_2:2716622-2716641 None:intergenic 30.0%
GGGGAACTAAAAAATTGTAT+TGG - chr2_2:2716627-2716646 Msa0230530:intron 30.0%
CTAAAAAATTGTATTGGAGG+AGG - chr2_2:2716633-2716652 Msa0230530:intron 30.0%
GTGGAAAATATGAGGAAAAA+TGG + chr2_2:2716908-2716927 None:intergenic 30.0%
AAAGTGAAGTGGAAAATATG+AGG + chr2_2:2716916-2716935 None:intergenic 30.0%
!!! TTTTGCAGTATAATTTGCTG+TGG - chr2_2:2717197-2717216 Msa0230530:CDS 30.0%
CATTGAAATATTTACTTGCC+AGG - chr2_2:2717248-2717267 Msa0230530:intron 30.0%
AACAAGAAATATATTCTCCC+TGG + chr2_2:2717269-2717288 None:intergenic 30.0%
TTTCTTGTTGCAAATTGCAT+TGG - chr2_2:2717280-2717299 Msa0230530:intron 30.0%
ATTATTGCTTGCTTAGGAGA+GGG + chr2_2:2714657-2714676 None:intergenic 35.0%
TCCTAAGCAAGCAATAATGT+CGG - chr2_2:2714659-2714678 Msa0230530:three_prime_UTR 35.0%
TTTCCTGAGAGATTCGATTT+GGG + chr2_2:2714694-2714713 None:intergenic 35.0%
ATTTCCTGAGAGATTCGATT+TGG + chr2_2:2714695-2714714 None:intergenic 35.0%
AAATCGAATCTCTCAGGAAA+TGG - chr2_2:2714694-2714713 Msa0230530:three_prime_UTR 35.0%
AATCGAATCTCTCAGGAAAT+GGG - chr2_2:2714695-2714714 Msa0230530:three_prime_UTR 35.0%
! TTTCTCGATTAGGTTGTTTG+TGG - chr2_2:2714835-2714854 Msa0230530:three_prime_UTR 35.0%
AATTTCGTTGCATTGCATGA+AGG - chr2_2:2715023-2715042 Msa0230530:CDS 35.0%
ATTTCGTTGCATTGCATGAA+GGG - chr2_2:2715024-2715043 Msa0230530:CDS 35.0%
!! GAGATTGTGAAGTTTTGATG+AGG - chr2_2:2715295-2715314 Msa0230530:intron 35.0%
!! GTTTTGATGAGGGTTACTAT+AGG - chr2_2:2715306-2715325 Msa0230530:intron 35.0%
!! AGTTTTCACTTTTGCCTGAT+TGG - chr2_2:2715378-2715397 Msa0230530:intron 35.0%
!!! GGTTTTGAATGGATTGGTTT+TGG - chr2_2:2715417-2715436 Msa0230530:intron 35.0%
!!! CTTTTTAGAGTCCTCAATTG+GGG - chr2_2:2715654-2715673 Msa0230530:intron 35.0%
!!! TGCGTTGTAGAAGCTTTTTT+TGG - chr2_2:2715716-2715735 Msa0230530:intron 35.0%
GAAAAATTGTGTTTCGCAAC+AGG - chr2_2:2715765-2715784 Msa0230530:intron 35.0%
! TGTTGTTTGATTCTAGTGCA+TGG - chr2_2:2716008-2716027 Msa0230530:intron 35.0%
CATTACTAATTAGATGGGCA+TGG - chr2_2:2716312-2716331 Msa0230530:intron 35.0%
TAATTAGATGGGCATGGAAA+TGG - chr2_2:2716318-2716337 Msa0230530:intron 35.0%
TGGAAATGGAGTGTAAAACA+AGG - chr2_2:2716332-2716351 Msa0230530:intron 35.0%
GTAAACTCTGCTACATAACA+CGG + chr2_2:2716422-2716441 None:intergenic 35.0%
TTTCCAATCACGGATAATAG+CGG + chr2_2:2716450-2716469 None:intergenic 35.0%
GGAAAAAAGGAATGAACAAG+TGG - chr2_2:2716529-2716548 Msa0230530:intron 35.0%
TGAACAAGTGGAAGAAATAG+AGG - chr2_2:2716541-2716560 Msa0230530:intron 35.0%
GTGGAAGAAATAGAGGAAAA+TGG - chr2_2:2716548-2716567 Msa0230530:intron 35.0%
AAGAAATAGAGGAAAATGGG+AGG - chr2_2:2716552-2716571 Msa0230530:intron 35.0%
AGAAATAGAGGAAAATGGGA+GGG - chr2_2:2716553-2716572 Msa0230530:intron 35.0%
AATACTAAACCCTCCTCATT+TGG - chr2_2:2716606-2716625 Msa0230530:intron 35.0%
ATACTAAACCCTCCTCATTT+GGG - chr2_2:2716607-2716626 Msa0230530:intron 35.0%
TTTGTGGAGTGGAAAATATG+AGG + chr2_2:2716814-2716833 None:intergenic 35.0%
!! TAAGGCTTTGTTTGTGAGTT+TGG + chr2_2:2716862-2716881 None:intergenic 35.0%
AACTCACAAACAAAGCCTTA+AGG - chr2_2:2716862-2716881 Msa0230530:intron 35.0%
ACAAAGCCTTAAGGATATGA+TGG - chr2_2:2716871-2716890 Msa0230530:intron 35.0%
CAAAGCCTTAAGGATATGAT+GGG - chr2_2:2716872-2716891 Msa0230530:intron 35.0%
ACAAACCCATCATATCCTTA+AGG + chr2_2:2716880-2716899 None:intergenic 35.0%
TGATCACAAGTCTGAAGAAA+AGG - chr2_2:2717054-2717073 Msa0230530:intron 35.0%
!! AATTTGCTGTGGTTCATCAA+TGG - chr2_2:2717208-2717227 Msa0230530:intron 35.0%
ACCACTTCATTTGTAACACT+TGG - chr2_2:2717535-2717554 Msa0230530:five_prime_UTR 35.0%
CCACTTCATTTGTAACACTT+GGG - chr2_2:2717536-2717555 Msa0230530:five_prime_UTR 35.0%
TGGGAAATACTATTGCGTGA+GGG + chr2_2:2714571-2714590 None:intergenic 40.0%
TTGGGAAATACTATTGCGTG+AGG + chr2_2:2714572-2714591 None:intergenic 40.0%
CATTATTGCTTGCTTAGGAG+AGG + chr2_2:2714658-2714677 None:intergenic 40.0%
TCCGACATTATTGCTTGCTT+AGG + chr2_2:2714663-2714682 None:intergenic 40.0%
AAACCCAAATCGAATCTCTC+AGG - chr2_2:2714688-2714707 Msa0230530:three_prime_UTR 40.0%
! TTAGGTTGTTTGTGGCTTAG+CGG - chr2_2:2714843-2714862 Msa0230530:three_prime_UTR 40.0%
TTTGTGGCTTAGCGGTATTA+CGG - chr2_2:2714851-2714870 Msa0230530:three_prime_UTR 40.0%
GGCGTGAATAATCTTAACAC+TGG + chr2_2:2714916-2714935 None:intergenic 40.0%
GTGTTAAGATTATTCACGCC+AGG - chr2_2:2714916-2714935 Msa0230530:three_prime_UTR 40.0%
!! ACTTTTGCCTGATTGGTTCT+GGG - chr2_2:2715385-2715404 Msa0230530:intron 40.0%
!!! GGACGAGAATTGGTTTTGAA+TGG - chr2_2:2715406-2715425 Msa0230530:intron 40.0%
! CAGGTTGAGCGAATAAATCA+AGG + chr2_2:2715830-2715849 None:intergenic 40.0%
CTTGATTTATTCGCTCAACC+TGG - chr2_2:2715828-2715847 Msa0230530:intron 40.0%
GCATGGCAAAACGAAAGTAA+GGG + chr2_2:2715868-2715887 None:intergenic 40.0%
TGCATGGCAAAACGAAAGTA+AGG + chr2_2:2715869-2715888 None:intergenic 40.0%
CAAGAGAAAACTCGAATGCA+TGG + chr2_2:2715885-2715904 None:intergenic 40.0%
ATGAAAGTAAGGGAGATGTG+AGG + chr2_2:2715911-2715930 None:intergenic 40.0%
CAAGGGGAAACTTGAATTCA+TGG + chr2_2:2715938-2715957 None:intergenic 40.0%
TCCAATTCTCAGATGGCAAT+TGG - chr2_2:2715975-2715994 Msa0230530:intron 40.0%
TCCAATTGCCATCTGAGAAT+TGG + chr2_2:2715979-2715998 None:intergenic 40.0%
CAGTATTCAATTGAGGAGAC+AGG + chr2_2:2716204-2716223 None:intergenic 40.0%
AAACCGCTATTATCCGTGAT+TGG - chr2_2:2716444-2716463 Msa0230530:intron 40.0%
TTATCCGTGATTGGAAACAC+TGG - chr2_2:2716453-2716472 Msa0230530:intron 40.0%
!! TAAGGCTTTGTTTGCGAGTT+TGG - chr2_2:2716493-2716512 Msa0230530:intron 40.0%
GAAATAGAGGAAAATGGGAG+GGG - chr2_2:2716554-2716573 Msa0230530:intron 40.0%
TACTAAACCCTCCTCATTTG+GGG - chr2_2:2716608-2716627 Msa0230530:intron 40.0%
! TTTAGTTCCCCAAATGAGGA+GGG + chr2_2:2716618-2716637 None:intergenic 40.0%
!!! TTTTAGTTCCCCAAATGAGG+AGG + chr2_2:2716619-2716638 None:intergenic 40.0%
! CTTTGTTTGTGAGTTTGGAG+GGG + chr2_2:2716857-2716876 None:intergenic 40.0%
!! GCTTTGTTTGTGAGTTTGGA+GGG + chr2_2:2716858-2716877 None:intergenic 40.0%
CAGCACAAGAAAAAGTGAAG+TGG + chr2_2:2716927-2716946 None:intergenic 40.0%
ACGCAAGTTCTTTAACTCCA+CGG - chr2_2:2717475-2717494 Msa0230530:five_prime_UTR 40.0%
CCCAAGTGTTACAAATGAAG+TGG + chr2_2:2717539-2717558 None:intergenic 40.0%
GAACACAAACTCCGTACTGT+TGG - chr2_2:2714723-2714742 Msa0230530:three_prime_UTR 45.0%
TTGTGGCTTAGCGGTATTAC+GGG - chr2_2:2714852-2714871 Msa0230530:three_prime_UTR 45.0%
CGGGTTCAATTGCGTACAAT+TGG - chr2_2:2714871-2714890 Msa0230530:three_prime_UTR 45.0%
!! CACTTTTGCCTGATTGGTTC+TGG - chr2_2:2715384-2715403 Msa0230530:intron 45.0%
!! ATTGGTTCTGGGACGAGAAT+TGG - chr2_2:2715396-2715415 Msa0230530:intron 45.0%
CTCAATTGGGGTAGTTGGAA+GGG - chr2_2:2715666-2715685 Msa0230530:intron 45.0%
TGAAACAATGCTGACGTGCT+TGG - chr2_2:2716054-2716073 Msa0230530:intron 45.0%
GACACCAGTGTTTCCAATCA+CGG + chr2_2:2716460-2716479 None:intergenic 45.0%
!! GCTTTGTTTGCGAGTTTGGA+GGG - chr2_2:2716497-2716516 Msa0230530:intron 45.0%
! CTTTGTTTGCGAGTTTGGAG+GGG - chr2_2:2716498-2716517 Msa0230530:intron 45.0%
AGGGGAAGGTTTGGGAAAAA+AGG - chr2_2:2716516-2716535 Msa0230530:intron 45.0%
! TGTATTGGAGGAGGGTTTTG+AGG - chr2_2:2716642-2716661 Msa0230530:intron 45.0%
!! GTATTGGAGGAGGGTTTTGA+GGG - chr2_2:2716643-2716662 Msa0230530:intron 45.0%
!!! TATTGGAGGAGGGTTTTGAG+GGG - chr2_2:2716644-2716663 Msa0230530:intron 45.0%
!! GGCTTTGTTTGTGAGTTTGG+AGG + chr2_2:2716859-2716878 None:intergenic 45.0%
CATGCTCAAGCTCTTACACT+GGG - chr2_2:2716998-2717017 Msa0230530:intron 45.0%
AGTCTGAAGAAAAGGCTGCA+AGG - chr2_2:2717062-2717081 Msa0230530:intron 45.0%
GTCTGAAGAAAAGGCTGCAA+GGG - chr2_2:2717063-2717082 Msa0230530:intron 45.0%
AAAAGGCTGCAAGGGATTCT+AGG - chr2_2:2717071-2717090 Msa0230530:intron 45.0%
GTATCTGACATCTCACAGCT+TGG - chr2_2:2717148-2717167 Msa0230530:CDS 45.0%
GTGGAGTTAAAGAACTTGCG+TGG + chr2_2:2717476-2717495 None:intergenic 45.0%
CGCAAGTTCTTTAACTCCAC+GGG - chr2_2:2717476-2717495 Msa0230530:five_prime_UTR 45.0%
GCAAGTTCTTTAACTCCACG+GGG - chr2_2:2717477-2717496 Msa0230530:five_prime_UTR 45.0%
TCATTTGTAACACTTGGGCG+TGG - chr2_2:2717541-2717560 Msa0230530:five_prime_UTR 45.0%
ATGACGCGGCGAACTACTAT+CGG + chr2_2:2714490-2714509 None:intergenic 50.0%
!! TGCTTCGTTGCTTGATGACG+CGG + chr2_2:2714504-2714523 None:intergenic 50.0%
TTCTCGTCCCAGAACCAATC+AGG + chr2_2:2715395-2715414 None:intergenic 50.0%
GAGTCCTCAATTGGGGTAGT+TGG - chr2_2:2715661-2715680 Msa0230530:intron 50.0%
CCTTCCAACTACCCCAATTG+AGG + chr2_2:2715668-2715687 None:intergenic 50.0%
CCTCAATTGGGGTAGTTGGA+AGG - chr2_2:2715665-2715684 Msa0230530:intron 50.0%
ACGAAAGTAAGGGAGATGCG+AGG + chr2_2:2715858-2715877 None:intergenic 50.0%
!! GGCTTTGTTTGCGAGTTTGG+AGG - chr2_2:2716496-2716515 Msa0230530:intron 50.0%
GAGTTTGGAGGGGAAGGTTT+GGG - chr2_2:2716508-2716527 Msa0230530:intron 50.0%
ATAGAGGAAAATGGGAGGGG+AGG - chr2_2:2716557-2716576 Msa0230530:intron 50.0%
TAGAGGAAAATGGGAGGGGA+GGG - chr2_2:2716558-2716577 Msa0230530:intron 50.0%
!!! AGGAGGGTTTTGAGGGGTTT+TGG - chr2_2:2716650-2716669 Msa0230530:intron 50.0%
!!! AGGGTTTTGAGGGGTTTTGG+AGG - chr2_2:2716653-2716672 Msa0230530:intron 50.0%
!!! GGGTTTTGAGGGGTTTTGGA+GGG - chr2_2:2716654-2716673 Msa0230530:intron 50.0%
! GAAGGAAAGGCTTTGTGGAG+TGG + chr2_2:2716825-2716844 None:intergenic 50.0%
TTTGTGAGTTTGGAGGGGAG+GGG + chr2_2:2716852-2716871 None:intergenic 50.0%
GTTTGTGAGTTTGGAGGGGA+GGG + chr2_2:2716853-2716872 None:intergenic 50.0%
TGTTTGTGAGTTTGGAGGGG+AGG + chr2_2:2716854-2716873 None:intergenic 50.0%
GCATGCTCAAGCTCTTACAC+TGG - chr2_2:2716997-2717016 Msa0230530:intron 50.0%
!! CTTACACTGGGAGCATTAGC+TGG - chr2_2:2717010-2717029 Msa0230530:intron 50.0%
TCTTTAACTCCACGGGGATG+AGG - chr2_2:2717483-2717502 Msa0230530:five_prime_UTR 50.0%
TAAGTTTCACCTCATCCCCG+TGG + chr2_2:2717495-2717514 None:intergenic 50.0%
GCGTCATCAAGCAACGAAGC+AGG - chr2_2:2714503-2714522 Msa0230530:three_prime_UTR 55.0%
AGGGAGATGCGAGGTATACC+AGG + chr2_2:2715849-2715868 None:intergenic 55.0%
GTTTGCGAGTTTGGAGGGGA+AGG - chr2_2:2716502-2716521 Msa0230530:intron 55.0%
CGAGTTTGGAGGGGAAGGTT+TGG - chr2_2:2716507-2716526 Msa0230530:intron 55.0%
AAAATGGGAGGGGAGGGCTT+TGG - chr2_2:2716564-2716583 Msa0230530:intron 55.0%
AAATGGGAGGGGAGGGCTTT+GGG - chr2_2:2716565-2716584 Msa0230530:intron 55.0%
GAGGGGAAGGAAAGGCTTTG+TGG + chr2_2:2716830-2716849 None:intergenic 55.0%
AGCATTAGCTGGAGCTGCAG+TGG - chr2_2:2717021-2717040 Msa0230530:intron 55.0%
AATGGGAGGGGAGGGCTTTG+GGG - chr2_2:2716566-2716585 Msa0230530:intron 60.0%
TGAGTTTGGAGGGGAGGGGA+GGG + chr2_2:2716848-2716867 None:intergenic 60.0%
! ATGGGAGGGGAGGGCTTTGG+GGG - chr2_2:2716567-2716586 Msa0230530:intron 65.0%
GAGTTTGGAGGGGAGGGGAG+GGG + chr2_2:2716847-2716866 None:intergenic 65.0%
GTGAGTTTGGAGGGGAGGGG+AGG + chr2_2:2716849-2716868 None:intergenic 65.0%
!! TGGGAGGGGAGGGCTTTGGG+GGG - chr2_2:2716568-2716587 Msa0230530:intron 70.0%
GGGGAGGGGAGGGGAAGGAA+AGG + chr2_2:2716838-2716857 None:intergenic 70.0%
TTGGAGGGGAGGGGAGGGGA+AGG + chr2_2:2716843-2716862 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 2714486 2717582 2714486 ID=Msa0230530;Name=Msa0230530
chr2_2 mRNA 2714486 2717582 2714486 ID=Msa0230530-mRNA-1;Parent=Msa0230530;Name=Msa0230530-mRNA-1;_AED=0.05;_eAED=0.05;_QI=189|1|1|1|1|1|3|483|88
chr2_2 exon 2714486 2715074 2714486 ID=Msa0230530-mRNA-1:exon:934;Parent=Msa0230530-mRNA-1
chr2_2 exon 2717131 2717221 2717131 ID=Msa0230530-mRNA-1:exon:933;Parent=Msa0230530-mRNA-1
chr2_2 exon 2717324 2717582 2717324 ID=Msa0230530-mRNA-1:exon:932;Parent=Msa0230530-mRNA-1
chr2_2 five_prime_UTR 2717394 2717582 2717394 ID=Msa0230530-mRNA-1:five_prime_utr;Parent=Msa0230530-mRNA-1
chr2_2 CDS 2717324 2717393 2717324 ID=Msa0230530-mRNA-1:cds;Parent=Msa0230530-mRNA-1
chr2_2 CDS 2717131 2717221 2717131 ID=Msa0230530-mRNA-1:cds;Parent=Msa0230530-mRNA-1
chr2_2 CDS 2714969 2715074 2714969 ID=Msa0230530-mRNA-1:cds;Parent=Msa0230530-mRNA-1
chr2_2 three_prime_UTR 2714486 2714968 2714486 ID=Msa0230530-mRNA-1:three_prime_utr;Parent=Msa0230530-mRNA-1
Gene Sequence

>Msa0230530

ATGTCGGATGCTAAAACCCAAATCGAATCTCTCAGGAAATGGGTCGTTGAACACAAACTCCGTACTGTTGGTTGTTTGTGGCTTAGCGGTATTACGGGTTCAATTGCGTACAATTGGTCTCGTCCTAACATGAAAACCAGTGTTAAGATTATTCACGCCAGGTTGCATGCTCAAGCTCTTACACTGGGAGCATTAGCTGGAGCTGCAGTGGTAGAATATTATGATCACAAGTCTGAAGAAAAGGCTGCAAGGGATTCTAGGCGATAA
Protein sequence

>Msa0230530

MSDAKTQIESLRKWVVEHKLRTVGCLWLSGITGSIAYNWSRPNMKTSVKIIHARLHAQALTLGALAGAAVVEYYDHKSEEKAARDSRR*