AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0176340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0176340 MtrunA17_Chr1g0198801 97.935 339 6 1 1 339 1 338 0.0 684
Msa0176340 MtrunA17_Chr7g0255961 50.847 177 83 2 142 317 58 231 3.74e-52 172
Msa0176340 MtrunA17_Chr3g0113721 42.661 218 109 5 124 331 9 220 3.89e-49 164
Msa0176340 MtrunA17_Chr4g0041051 41.146 192 102 2 155 339 64 251 3.96e-32 120
Msa0176340 MtrunA17_Chr2g0325521 42.458 179 92 3 145 316 45 219 1.92e-30 115
Msa0176340 MtrunA17_Chr5g0412371 36.145 166 89 3 157 316 43 197 5.75e-30 113
Msa0176340 MtrunA17_Chr5g0427361 34.934 229 107 4 136 327 8 231 5.76e-27 107
Msa0176340 MtrunA17_Chr4g0045901 43.407 182 89 6 143 317 62 236 9.16e-25 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0176340 AT1G68810.1 64.773 264 67 8 96 337 99 358 9.37e-95 286
Msa0176340 AT3G25710.1 50.000 320 98 8 71 333 22 336 1.00e-79 247
Msa0176340 AT3G56770.1 47.059 170 82 4 151 317 37 201 7.31e-43 148
Msa0176340 AT2G41130.1 46.629 178 84 5 143 317 55 224 2.83e-38 137
Msa0176340 AT2G40200.1 42.105 190 89 8 130 311 38 214 1.32e-33 125
Msa0176340 AT4G38070.1 37.324 142 77 3 153 294 87 216 3.19e-25 102
Msa0176340 AT1G01260.1 30.000 180 101 8 38 207 314 478 9.41e-11 63.5
Msa0176340 AT1G01260.2 30.000 180 101 8 38 207 314 478 9.41e-11 63.5
Msa0176340 AT1G01260.3 30.000 180 101 8 38 207 314 478 9.41e-11 63.5

Find 80 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAGAATTGATTAAATTA+TGG 0.270913 1_4:+62693868 Msa0176340:exon
ATTATTATTATATTAGTATA+TGG 0.273119 1_4:+62693921 Msa0176340:three_prime_UTR
ATGCAAGTTTGTTCAATAGA+AGG 0.283417 1_4:+62692065 Msa0176340:CDS
GATGAGTGCTCAAGAAATTA+TGG 0.286315 1_4:+62692213 Msa0176340:CDS
TCAAGACATTGAAAGCATTA+AGG 0.296728 1_4:+62693640 Msa0176340:CDS
TGTTCTAATTGATTCTATAA+TGG 0.302929 1_4:-62691220 None:intergenic
TTCAATGTCTTGATTAGTTC+AGG 0.303436 1_4:-62693630 None:intergenic
ATGCAGGTTCTGAGCATCTT+AGG 0.326044 1_4:+62692119 Msa0176340:CDS
TATACTAATATAATAATAAT+AGG 0.326223 1_4:-62693918 None:intergenic
GTTGTAGATGAATCTGCTTC+TGG 0.328817 1_4:+62693801 Msa0176340:CDS
GCAGATGATGATGAAGATTA+TGG 0.331671 1_4:+62693543 Msa0176340:CDS
AATAGTGATCATCATATCTT+TGG 0.353704 1_4:+62691884 Msa0176340:CDS
TGATCTTCTTGTATCATACT+TGG 0.359086 1_4:-62691773 None:intergenic
ACAATGAGGTAATGATAATT+TGG 0.372247 1_4:+62694093 Msa0176340:three_prime_UTR
GATGAAGATTATGGATCAAA+TGG 0.416803 1_4:+62693552 Msa0176340:CDS
TTCAGCTTCACTATGACTCT+TGG 0.417321 1_4:-62692257 None:intergenic
TTATATTAGTATATGGTACT+TGG 0.421823 1_4:+62693928 Msa0176340:three_prime_UTR
TCATCACATTCAGTTGGAAC+TGG 0.424698 1_4:-62693504 None:intergenic
GTTTGTTTCTCTTGTGGTGG+TGG 0.425567 1_4:-62691169 None:intergenic
TGTTGTTGAGGAATAACAAA+AGG 0.437987 1_4:-62692031 None:intergenic
CCTCACTTTGCTGTGATGAT+AGG 0.438078 1_4:+62693595 Msa0176340:CDS
TTCTTTGTTTGTTTCTCTTG+TGG 0.438449 1_4:-62691175 None:intergenic
GGATCAGGTTCAGGACCCTT+TGG 0.439350 1_4:+62692166 Msa0176340:CDS
CTATTGAACAAACTTGCATA+AGG 0.450006 1_4:-62692061 None:intergenic
ATACTTACTTTGGTTGTGCT+TGG 0.452519 1_4:-62692334 None:intergenic
GAGAGATAAATCTAAAGCCT+TGG 0.462647 1_4:+62691130 Msa0176340:five_prime_UTR
CTTCAACATGGATCAGGTTC+AGG 0.466813 1_4:+62692157 Msa0176340:CDS
AGAGAGCAACAAAGCACAAA+GGG 0.469426 1_4:+62690989 Msa0176340:five_prime_UTR
GCCTCATCATCTGTTGTTGA+AGG 0.470688 1_4:-62691856 None:intergenic
GACCATGTTTGTTGCAAGAC+TGG 0.480750 1_4:-62691959 None:intergenic
TGCAAGTTTGTTCAATAGAA+GGG 0.484018 1_4:+62692066 Msa0176340:CDS
GTAAGATGTTGTTATGTGTA+TGG 0.487450 1_4:+62694048 Msa0176340:three_prime_UTR
CTAATTGATTCTATAATGGT+GGG 0.495009 1_4:-62691216 None:intergenic
GTTAATTCATCACATTCAGT+TGG 0.495856 1_4:-62693510 None:intergenic
TCTAATTGATTCTATAATGG+TGG 0.508231 1_4:-62691217 None:intergenic
ATGAGGTAATGATAATTTGG+TGG 0.510319 1_4:+62694096 Msa0176340:three_prime_UTR
GCAACAAAGCACAAAGGGAA+AGG 0.511848 1_4:+62690994 Msa0176340:five_prime_UTR
TCAGGTTCAGGACCCTTTGG+TGG 0.514868 1_4:+62692169 Msa0176340:CDS
TGATCACCTCCTCCTGCTGT+AGG 0.517327 1_4:-62691926 None:intergenic
TCAAATCTCTTTCACTTGGT+AGG 0.518755 1_4:-62691076 None:intergenic
TCACTACGCACAAATGTTTG+TGG 0.519274 1_4:+62691044 Msa0176340:five_prime_UTR
TTTGTTTGTTTCTCTTGTGG+TGG 0.520406 1_4:-62691172 None:intergenic
AGTGATCATCATATCTTTGG+TGG 0.524209 1_4:+62691887 Msa0176340:CDS
AGGTCTATGTTCCCTACAGC+AGG 0.528893 1_4:+62691914 Msa0176340:CDS
GCTGATATAACAACACTTGG+AGG 0.532615 1_4:+62693678 Msa0176340:CDS
TTTGTTTCTCTTGTGGTGGT+GGG 0.534262 1_4:-62691168 None:intergenic
AAAGGGTCATGAGGATTGAA+AGG 0.537899 1_4:-62692013 None:intergenic
CACCAGTCTTGCAACAAACA+TGG 0.540530 1_4:+62691957 Msa0176340:CDS
GTTGTTGAGGAATAACAAAA+GGG 0.542912 1_4:-62692030 None:intergenic
TTTGCATATGATCATCATGC+AGG 0.543634 1_4:+62692103 Msa0176340:CDS
TCAAGAAATTATGGAGGCTA+AGG 0.546278 1_4:+62692222 Msa0176340:CDS
GATCACCTCCTCCTGCTGTA+GGG 0.548133 1_4:-62691925 None:intergenic
AATTGATTCTATAATGGTGG+GGG 0.552128 1_4:-62691214 None:intergenic
GATACACTTCAACATGGATC+AGG 0.552437 1_4:+62692151 Msa0176340:CDS
TAATTGATTCTATAATGGTG+GGG 0.553540 1_4:-62691215 None:intergenic
ATCATATCTTTGGTGGAGGA+AGG 0.562826 1_4:+62691894 Msa0176340:CDS
TCATATGCAAACTGAAGAGA+AGG 0.572922 1_4:-62692091 None:intergenic
AGAAGCACTCAAAGCTGTGA+TGG 0.573378 1_4:+62693770 Msa0176340:CDS
AGCTGCGTAGCTTAGCAAGA+TGG 0.576612 1_4:-62692306 None:intergenic
TCCTTCAACAACAGATGATG+AGG 0.577140 1_4:+62691855 Msa0176340:CDS
GATCATCATATCTTTGGTGG+AGG 0.579767 1_4:+62691890 Msa0176340:CDS
ATGTTCCCTACAGCAGGAGG+AGG 0.581224 1_4:+62691920 Msa0176340:CDS
TTCACCTTGAAAACCACCAA+AGG 0.584286 1_4:-62692182 None:intergenic
TCACCTTGAAAACCACCAAA+GGG 0.586225 1_4:-62692181 None:intergenic
AATGTCAAATCTCTTTCACT+TGG 0.587124 1_4:-62691080 None:intergenic
TAAGGTGAAGCTTGTTGTTG+AGG 0.593376 1_4:-62692043 None:intergenic
ATTAGAACAATATCTATCAA+AGG 0.593991 1_4:+62691234 Msa0176340:five_prime_UTR
AAGAGAGCAACAAAGCACAA+AGG 0.595215 1_4:+62690988 Msa0176340:five_prime_UTR
AGAGTCATAGTGAAGCTGAA+AGG 0.601072 1_4:+62692260 Msa0176340:CDS
GTTTCTCTTGTGGTGGTGGG+CGG 0.602754 1_4:-62691165 None:intergenic
AAAGCTGATATAACAACACT+TGG 0.602897 1_4:+62693675 Msa0176340:CDS
CTGGAAGTGTCAAGAGACAA+AGG 0.603991 1_4:+62693820 Msa0176340:CDS
GGAATAACAAAAGGGTCATG+AGG 0.626831 1_4:-62692022 None:intergenic
CCTATCATCACAGCAAAGTG+AGG 0.634985 1_4:-62693595 None:intergenic
AGTATGATACAAGAAGATCA+AGG 0.636259 1_4:+62691776 Msa0176340:exon
TCTATGTTCCCTACAGCAGG+AGG 0.647545 1_4:+62691917 Msa0176340:CDS
GGCGGCCAAAGAAAAGTCCA+AGG 0.663085 1_4:-62691147 None:intergenic
ATATCAGATACACTTCAACA+TGG 0.699194 1_4:+62692145 Msa0176340:CDS
GAGTGCTCAAGAAATTATGG+AGG 0.703562 1_4:+62692216 Msa0176340:CDS
AACATGATGATGAGACAATG+AGG 0.726295 1_4:+62694079 Msa0176340:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATATTAGTACTATAT+TGG - chr1_4:62692497-62692516 None:intergenic 10.0%
!! AAATTTATAGTTAATTAAAG+TGG - chr1_4:62693315-62693334 None:intergenic 10.0%
!! AATTAGAATTGATTAAATTA+TGG + chr1_4:62693868-62693887 Msa0176340:exon 10.0%
!!! TTTTTATCTTTTTTTTTTTG+GGG + chr1_4:62693983-62694002 Msa0176340:three_prime_UTR 10.0%
!! ATATATACATATGAATATGA+TGG - chr1_4:62691706-62691725 None:intergenic 15.0%
!! ATGTATATATATATAAACCT+TGG + chr1_4:62691716-62691735 Msa0176340:intron 15.0%
!!! TTTTATCTTTTTTTTTTTGG+GGG + chr1_4:62693984-62694003 Msa0176340:three_prime_UTR 15.0%
!!! TGTTCTAATTGATTCTATAA+TGG - chr1_4:62691223-62691242 None:intergenic 20.0%
!! ATTAGAACAATATCTATCAA+AGG + chr1_4:62691234-62691253 Msa0176340:five_prime_UTR 20.0%
!! ACAAGGAATAAAAATGAAAA+GGG - chr1_4:62691329-62691348 None:intergenic 20.0%
!!! TAAGCAAAAATCTACTTTTT+AGG + chr1_4:62691472-62691491 Msa0176340:intron 20.0%
!! CATTTAATAAATGATGTATG+TGG - chr1_4:62691536-62691555 None:intergenic 20.0%
!! ATATATATATATACATCCTC+CGG - chr1_4:62691593-62691612 None:intergenic 20.0%
!! TTACTACTATATACTTACTT+TGG - chr1_4:62692347-62692366 None:intergenic 20.0%
!!! AGATATTTTGAATCTACAAA+AGG - chr1_4:62692603-62692622 None:intergenic 20.0%
!!! GATTTTGATTTTGTTGTTTA+TGG - chr1_4:62692638-62692657 None:intergenic 20.0%
!!! ATATAACCTTTTAGAAAATG+AGG + chr1_4:62692660-62692679 Msa0176340:intron 20.0%
!!! TGTAACTATATGATTTGTTT+TGG + chr1_4:62692794-62692813 Msa0176340:intron 20.0%
!!! GTAACTATATGATTTGTTTT+GGG + chr1_4:62692795-62692814 Msa0176340:intron 20.0%
!!! AATAGTGGATATATTTTGAA+TGG - chr1_4:62693102-62693121 None:intergenic 20.0%
!! TATGATATAAGAATGTGAAA+TGG - chr1_4:62693126-62693145 None:intergenic 20.0%
!! ATAGATCTGATATATACATA+AGG - chr1_4:62693167-62693186 None:intergenic 20.0%
!! TGAAAATATTTACAATCTCA+TGG - chr1_4:62693200-62693219 None:intergenic 20.0%
!! TTATATTAGTATATGGTACT+TGG + chr1_4:62693928-62693947 Msa0176340:three_prime_UTR 20.0%
!!! TCTTTTCTTTTCTTTTTTCA+TGG + chr1_4:62694019-62694038 Msa0176340:three_prime_UTR 20.0%
!! TAATTGATTCTATAATGGTG+GGG - chr1_4:62691218-62691237 None:intergenic 25.0%
!! CTAATTGATTCTATAATGGT+GGG - chr1_4:62691219-62691238 None:intergenic 25.0%
!! TCTAATTGATTCTATAATGG+TGG - chr1_4:62691220-62691239 None:intergenic 25.0%
!!! AAATGAAAAGGGTATTTGAA+AGG - chr1_4:62691318-62691337 None:intergenic 25.0%
! CACAAGGAATAAAAATGAAA+AGG - chr1_4:62691330-62691349 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr1_4:62691462-62691481 None:intergenic 25.0%
! CATTCAATAAATGATGTATG+TGG + chr1_4:62691497-62691516 Msa0176340:intron 25.0%
!! ATTTTGCTTATAATAGTGAC+CGG + chr1_4:62691571-62691590 Msa0176340:intron 25.0%
! ACAAAATAAAACCTAGCTAT+AGG + chr1_4:62691649-62691668 Msa0176340:intron 25.0%
! TATATATAAACCTTGGAGTT+TGG + chr1_4:62691723-62691742 Msa0176340:intron 25.0%
! AATAGTGATCATCATATCTT+TGG + chr1_4:62691884-62691903 Msa0176340:CDS 25.0%
!! GGTTTGCATTTTAAATTTCT+TGG - chr1_4:62692453-62692472 None:intergenic 25.0%
! GGCATATATTGAATTCAAAA+AGG - chr1_4:62692582-62692601 None:intergenic 25.0%
! TGAAAATATATTGTCTTTGC+CGG + chr1_4:62692959-62692978 Msa0176340:intron 25.0%
! TGGAGAATGAAAAGAAATAA+AGG + chr1_4:62693009-62693028 Msa0176340:intron 25.0%
! ATCTGATATATACATAAGGT+TGG - chr1_4:62693163-62693182 None:intergenic 25.0%
! ATATTTACAATCTCATGGTA+GGG - chr1_4:62693195-62693214 None:intergenic 25.0%
! AATATTTACAATCTCATGGT+AGG - chr1_4:62693196-62693215 None:intergenic 25.0%
!!! AAGAATGTTTTGTTCATTAC+TGG + chr1_4:62693708-62693727 Msa0176340:CDS 25.0%
! ACAATGAGGTAATGATAATT+TGG + chr1_4:62694093-62694112 Msa0176340:three_prime_UTR 25.0%
!!! GTTTTATTTTTACATGTGTG+TGG + chr1_4:62694118-62694137 Msa0176340:three_prime_UTR 25.0%
AATGTCAAATCTCTTTCACT+TGG - chr1_4:62691083-62691102 None:intergenic 30.0%
! TTCTTTGTTTGTTTCTCTTG+TGG - chr1_4:62691178-62691197 None:intergenic 30.0%
! AATTGATTCTATAATGGTGG+GGG - chr1_4:62691217-62691236 None:intergenic 30.0%
GGTATTTGAAAGGACAAAAT+GGG - chr1_4:62691308-62691327 None:intergenic 30.0%
! TGTTTTATATCCAAACTCCA+AGG - chr1_4:62691736-62691755 None:intergenic 30.0%
TTGGAGTTTGGATATAAAAC+AGG + chr1_4:62691735-62691754 Msa0176340:intron 30.0%
TGATCTTCTTGTATCATACT+TGG - chr1_4:62691776-62691795 None:intergenic 30.0%
AGTATGATACAAGAAGATCA+AGG + chr1_4:62691776-62691795 Msa0176340:exon 30.0%
GTTGTTGAGGAATAACAAAA+GGG - chr1_4:62692033-62692052 None:intergenic 30.0%
TGTTGTTGAGGAATAACAAA+AGG - chr1_4:62692034-62692053 None:intergenic 30.0%
CTATTGAACAAACTTGCATA+AGG - chr1_4:62692064-62692083 None:intergenic 30.0%
! ATGCAAGTTTGTTCAATAGA+AGG + chr1_4:62692065-62692084 Msa0176340:CDS 30.0%
! TGCAAGTTTGTTCAATAGAA+GGG + chr1_4:62692066-62692085 Msa0176340:CDS 30.0%
ATATCAGATACACTTCAACA+TGG + chr1_4:62692145-62692164 Msa0176340:CDS 30.0%
!! TTGAGTTGAGATCTTTGATT+AGG - chr1_4:62692474-62692493 None:intergenic 30.0%
! TTTGCACCTCATTTTCTAAA+AGG - chr1_4:62692669-62692688 None:intergenic 30.0%
! GCTTTTGTTAACATCCTTTT+TGG + chr1_4:62692708-62692727 Msa0176340:intron 30.0%
GTATACAAAACAAGCCAAAA+AGG - chr1_4:62692725-62692744 None:intergenic 30.0%
GAATTAGTGTTAATGTCTCT+TGG + chr1_4:62692890-62692909 Msa0176340:intron 30.0%
TGACTGATCAACATATTATC+CGG - chr1_4:62692981-62693000 None:intergenic 30.0%
!! AGTGGATATATTTTGAATGG+TGG - chr1_4:62693099-62693118 None:intergenic 30.0%
AGAATGTGAAATGGAAATAG+TGG - chr1_4:62693117-62693136 None:intergenic 30.0%
! GATATATACATAAGGTTGGT+AGG - chr1_4:62693159-62693178 None:intergenic 30.0%
GTTAATTCATCACATTCAGT+TGG - chr1_4:62693513-62693532 None:intergenic 30.0%
GATGAAGATTATGGATCAAA+TGG + chr1_4:62693552-62693571 Msa0176340:CDS 30.0%
! TTCAATGTCTTGATTAGTTC+AGG - chr1_4:62693633-62693652 None:intergenic 30.0%
!! TCAAGACATTGAAAGCATTA+AGG + chr1_4:62693640-62693659 Msa0176340:CDS 30.0%
AAAGCTGATATAACAACACT+TGG + chr1_4:62693675-62693694 Msa0176340:CDS 30.0%
!!! TATCTTTTTTTTTTTGGGGG+TGG + chr1_4:62693987-62694006 Msa0176340:three_prime_UTR 30.0%
GTAAGATGTTGTTATGTGTA+TGG + chr1_4:62694048-62694067 Msa0176340:three_prime_UTR 30.0%
ATGAGGTAATGATAATTTGG+TGG + chr1_4:62694096-62694115 Msa0176340:three_prime_UTR 30.0%
TCAAATCTCTTTCACTTGGT+AGG - chr1_4:62691079-62691098 None:intergenic 35.0%
! GAGAGATAAATCTAAAGCCT+TGG + chr1_4:62691130-62691149 Msa0176340:five_prime_UTR 35.0%
! TAAAGCCTTGGACTTTTCTT+TGG + chr1_4:62691142-62691161 Msa0176340:five_prime_UTR 35.0%
! TTTGTTTGTTTCTCTTGTGG+TGG - chr1_4:62691175-62691194 None:intergenic 35.0%
GGGTATTTGAAAGGACAAAA+TGG - chr1_4:62691309-62691328 None:intergenic 35.0%
TATAGGCACTTAATTGCACA+AGG - chr1_4:62691346-62691365 None:intergenic 35.0%
GTCATAATATATACACCCTC+CGG + chr1_4:62691440-62691459 Msa0176340:intron 35.0%
AAATGTCAGAACCTATAGCT+AGG - chr1_4:62691663-62691682 None:intergenic 35.0%
AGTGATCATCATATCTTTGG+TGG + chr1_4:62691887-62691906 Msa0176340:CDS 35.0%
TCATATGCAAACTGAAGAGA+AGG - chr1_4:62692094-62692113 None:intergenic 35.0%
TTTGCATATGATCATCATGC+AGG + chr1_4:62692103-62692122 Msa0176340:CDS 35.0%
GATGAGTGCTCAAGAAATTA+TGG + chr1_4:62692213-62692232 Msa0176340:CDS 35.0%
TCAAGAAATTATGGAGGCTA+AGG + chr1_4:62692222-62692241 Msa0176340:CDS 35.0%
ATACTTACTTTGGTTGTGCT+TGG - chr1_4:62692337-62692356 None:intergenic 35.0%
!!! GCAAGAGTGTTTTTTCTAAG+TGG + chr1_4:62692556-62692575 Msa0176340:intron 35.0%
TATAATGTGTGGTAGTTCCA+AGG + chr1_4:62692826-62692845 Msa0176340:intron 35.0%
TGTCTCTTGGTATGATAAAG+AGG + chr1_4:62692903-62692922 Msa0176340:intron 35.0%
!! TTTTCAACTGCATGCTGTTT+TGG - chr1_4:62692931-62692950 None:intergenic 35.0%
GTTGATCAGTCAAACTCAAT+TGG + chr1_4:62692989-62693008 Msa0176340:intron 35.0%
GTTATGTTTGTGACTGACAA+AGG - chr1_4:62693053-62693072 None:intergenic 35.0%
! GCAGATGATGATGAAGATTA+TGG + chr1_4:62693543-62693562 Msa0176340:CDS 35.0%
! AACATGATGATGAGACAATG+AGG + chr1_4:62694079-62694098 Msa0176340:three_prime_UTR 35.0%
!! GTGCTTTGTTGCTCTCTTTT+TGG - chr1_4:62690986-62691005 None:intergenic 40.0%
AAGAGAGCAACAAAGCACAA+AGG + chr1_4:62690988-62691007 Msa0176340:five_prime_UTR 40.0%
AGAGAGCAACAAAGCACAAA+GGG + chr1_4:62690989-62691008 Msa0176340:five_prime_UTR 40.0%
TCACTACGCACAAATGTTTG+TGG + chr1_4:62691044-62691063 Msa0176340:five_prime_UTR 40.0%
! TTTGTTTCTCTTGTGGTGGT+GGG - chr1_4:62691171-62691190 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr1_4:62691575-62691594 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr1_4:62691574-62691593 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr1_4:62691574-62691593 Msa0176340:intron 40.0%
TCCTTCAACAACAGATGATG+AGG + chr1_4:62691855-62691874 Msa0176340:CDS 40.0%
GATCATCATATCTTTGGTGG+AGG + chr1_4:62691890-62691909 Msa0176340:CDS 40.0%
ATCATATCTTTGGTGGAGGA+AGG + chr1_4:62691894-62691913 Msa0176340:CDS 40.0%
AAAGGGTCATGAGGATTGAA+AGG - chr1_4:62692016-62692035 None:intergenic 40.0%
GGAATAACAAAAGGGTCATG+AGG - chr1_4:62692025-62692044 None:intergenic 40.0%
!! TAAGGTGAAGCTTGTTGTTG+AGG - chr1_4:62692046-62692065 None:intergenic 40.0%
GATACACTTCAACATGGATC+AGG + chr1_4:62692151-62692170 Msa0176340:CDS 40.0%
TCACCTTGAAAACCACCAAA+GGG - chr1_4:62692184-62692203 None:intergenic 40.0%
TTCACCTTGAAAACCACCAA+AGG - chr1_4:62692185-62692204 None:intergenic 40.0%
GAGTGCTCAAGAAATTATGG+AGG + chr1_4:62692216-62692235 Msa0176340:CDS 40.0%
TTCAGCTTCACTATGACTCT+TGG - chr1_4:62692260-62692279 None:intergenic 40.0%
AGAGTCATAGTGAAGCTGAA+AGG + chr1_4:62692260-62692279 Msa0176340:CDS 40.0%
CTAGCTGGCTAAAGAAAAAG+TGG + chr1_4:62692855-62692874 Msa0176340:intron 40.0%
TCATCACATTCAGTTGGAAC+TGG - chr1_4:62693507-62693526 None:intergenic 40.0%
GCTGATATAACAACACTTGG+AGG + chr1_4:62693678-62693697 Msa0176340:CDS 40.0%
GTTGTAGATGAATCTGCTTC+TGG + chr1_4:62693801-62693820 Msa0176340:CDS 40.0%
GCAACAAAGCACAAAGGGAA+AGG + chr1_4:62690994-62691013 Msa0176340:five_prime_UTR 45.0%
! GTTTGTTTCTCTTGTGGTGG+TGG - chr1_4:62691172-62691191 None:intergenic 45.0%
GCCTCATCATCTGTTGTTGA+AGG - chr1_4:62691859-62691878 None:intergenic 45.0%
CACCAGTCTTGCAACAAACA+TGG + chr1_4:62691957-62691976 Msa0176340:CDS 45.0%
! GACCATGTTTGTTGCAAGAC+TGG - chr1_4:62691962-62691981 None:intergenic 45.0%
!! ATGCAGGTTCTGAGCATCTT+AGG + chr1_4:62692119-62692138 Msa0176340:CDS 45.0%
CTTCAACATGGATCAGGTTC+AGG + chr1_4:62692157-62692176 Msa0176340:CDS 45.0%
!! GGTGGTTTTCAAGGTGAAGT+TGG + chr1_4:62692187-62692206 Msa0176340:CDS 45.0%
!! GGGAGTGTCTCTATAATGTG+TGG + chr1_4:62692815-62692834 Msa0176340:intron 45.0%
CCTATCATCACAGCAAAGTG+AGG - chr1_4:62693598-62693617 None:intergenic 45.0%
! CCTCACTTTGCTGTGATGAT+AGG + chr1_4:62693595-62693614 Msa0176340:CDS 45.0%
!! AGAAGCACTCAAAGCTGTGA+TGG + chr1_4:62693770-62693789 Msa0176340:CDS 45.0%
!! CTGGAAGTGTCAAGAGACAA+AGG + chr1_4:62693820-62693839 Msa0176340:CDS 45.0%
!! AATAAAAAAAAAATCTATAT+AGG - chr1_4:62691363-62691382 None:intergenic 5.0%
!!! TATAAATTTTATCTAATTAA+AGG + chr1_4:62693895-62693914 Msa0176340:three_prime_UTR 5.0%
!! TATACTAATATAATAATAAT+AGG - chr1_4:62693921-62693940 None:intergenic 5.0%
!! ATTATTATTATATTAGTATA+TGG + chr1_4:62693921-62693940 Msa0176340:three_prime_UTR 5.0%
!!! GTTATTATATATTTTTTATT+AGG + chr1_4:62693950-62693969 Msa0176340:three_prime_UTR 5.0%
!!! AATTTTTATCTTTTTTTTTT+TGG + chr1_4:62693981-62694000 Msa0176340:three_prime_UTR 5.0%
!!! ATTTTTATCTTTTTTTTTTT+GGG + chr1_4:62693982-62694001 Msa0176340:three_prime_UTR 5.0%
AGGTCTATGTTCCCTACAGC+AGG + chr1_4:62691914-62691933 Msa0176340:CDS 50.0%
TCTATGTTCCCTACAGCAGG+AGG + chr1_4:62691917-62691936 Msa0176340:CDS 50.0%
!! GGACCCTTTGGTGGTTTTCA+AGG + chr1_4:62692178-62692197 Msa0176340:CDS 50.0%
AGCTGCGTAGCTTAGCAAGA+TGG - chr1_4:62692309-62692328 None:intergenic 50.0%
TGAGCTGAGCTGAGATCTCA+TGG - chr1_4:62693226-62693245 None:intergenic 50.0%
GGCGGCCAAAGAAAAGTCCA+AGG - chr1_4:62691150-62691169 None:intergenic 55.0%
!! GTTTCTCTTGTGGTGGTGGG+CGG - chr1_4:62691168-62691187 None:intergenic 55.0%
ATGTTCCCTACAGCAGGAGG+AGG + chr1_4:62691920-62691939 Msa0176340:CDS 55.0%
GATCACCTCCTCCTGCTGTA+GGG - chr1_4:62691928-62691947 None:intergenic 55.0%
TGATCACCTCCTCCTGCTGT+AGG - chr1_4:62691929-62691948 None:intergenic 55.0%
GGATCAGGTTCAGGACCCTT+TGG + chr1_4:62692166-62692185 Msa0176340:CDS 55.0%
TCAGGTTCAGGACCCTTTGG+TGG + chr1_4:62692169-62692188 Msa0176340:CDS 55.0%
GTTCCAAGGAAGCAGCTAGC+TGG + chr1_4:62692840-62692859 Msa0176340:intron 55.0%
TAGCCAGCTAGCTGCTTCCT+TGG - chr1_4:62692846-62692865 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 62690968 62694147 62690968 ID=Msa0176340;Name=Msa0176340
chr1_4 mRNA 62690968 62694147 62690968 ID=Msa0176340-mRNA-1;Parent=Msa0176340;Name=Msa0176340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=310|1|1|1|0.5|0.33|3|278|339
chr1_4 exon 62690968 62691255 62690968 ID=Msa0176340-mRNA-1:exon:20184;Parent=Msa0176340-mRNA-1
chr1_4 exon 62691757 62692348 62691757 ID=Msa0176340-mRNA-1:exon:20185;Parent=Msa0176340-mRNA-1
chr1_4 exon 62693420 62694147 62693420 ID=Msa0176340-mRNA-1:exon:20186;Parent=Msa0176340-mRNA-1
chr1_4 five_prime_UTR 62690968 62691255 62690968 ID=Msa0176340-mRNA-1:five_prime_utr;Parent=Msa0176340-mRNA-1
chr1_4 five_prime_UTR 62691757 62691778 62691757 ID=Msa0176340-mRNA-1:five_prime_utr;Parent=Msa0176340-mRNA-1
chr1_4 CDS 62691779 62692348 62691779 ID=Msa0176340-mRNA-1:cds;Parent=Msa0176340-mRNA-1
chr1_4 CDS 62693420 62693869 62693420 ID=Msa0176340-mRNA-1:cds;Parent=Msa0176340-mRNA-1
chr1_4 three_prime_UTR 62693870 62694147 62693870 ID=Msa0176340-mRNA-1:three_prime_utr;Parent=Msa0176340-mRNA-1
Gene Sequence

>Msa0176340

ATGATACAAGAAGATCAAGGACAGTGTTCATCTCAAACAATCAATAACTTTGAAACTTATCAACAAGAGCAGTTTCTCCTTCAACAACAGATGATGAGGCAACAAAATAGTGATCATCATATCTTTGGTGGAGGAAGGTCTATGTTCCCTACAGCAGGAGGAGGTGATCATCAAGTTTCACCAGTCTTGCAACAAACATGGTCTATGCAACAACTTCATCATCATCATCATCATCCTTTCAATCCTCATGACCCTTTTGTTATTCCTCAACAACAAGCTTCACCTTATGCAAGTTTGTTCAATAGAAGGGTTCCTTCTCTTCAGTTTGCATATGATCATCATGCAGGTTCTGAGCATCTTAGGATCATATCAGATACACTTCAACATGGATCAGGTTCAGGACCCTTTGGTGGTTTTCAAGGTGAAGTTGGAAAGATGAGTGCTCAAGAAATTATGGAGGCTAAGGCTCTTGCTGCTTCCAAGAGTCATAGTGAAGCTGAAAGGAGACGCAGAGAGAGAATCAATAACCATCTTGCTAAGCTACGCAGCTTGTTGCCAAGCACAACCAAAACAGACAAAGCATCATTGCTAGCAGAAGTGATACAACATGTAAAAGAATTGAAGCGTCAAACATCGTTGATAGCAGAAACAAGTCCAGTTCCAACTGAATGTGATGAATTAACAGTAGATGCTGCAGATGATGATGAAGATTATGGATCAAATGGAAACAAGTTTATCATAAAAGCCTCACTTTGCTGTGATGATAGGTCAGATCTTTTGCCTGAACTAATCAAGACATTGAAAGCATTAAGGTTAAGAACACTCAAAGCTGATATAACAACACTTGGAGGACGTGTTAAGAATGTTTTGTTCATTACTGGTGAAGAAGATGATCATGAATATTGCATTAGTTCAATACAAGAAGCACTCAAAGCTGTGATGGAGAAGAGTGTTGTAGATGAATCTGCTTCTGGAAGTGTCAAGAGACAAAGGACTAATATAATATCAATATCTAATTAA
Protein sequence

>Msa0176340

MIQEDQGQCSSQTINNFETYQQEQFLLQQQMMRQQNSDHHIFGGGRSMFPTAGGGDHQVSPVLQQTWSMQQLHHHHHHPFNPHDPFVIPQQQASPYASLFNRRVPSLQFAYDHHAGSEHLRIISDTLQHGSGSGPFGGFQGEVGKMSAQEIMEAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPVPTECDELTVDAADDDEDYGSNGNKFIIKASLCCDDRSDLLPELIKTLKALRLRTLKADITTLGGRVKNVLFITGEEDDHEYCISSIQEALKAVMEKSVVDESASGSVKRQRTNIISISN*