AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0084420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084420 MtrunA17_Chr1g0198801 97.935 339 6 1 1 339 1 338 0.0 684
Msa0084420 MtrunA17_Chr7g0255961 50.847 177 83 2 142 317 58 231 3.74e-52 172
Msa0084420 MtrunA17_Chr3g0113721 42.661 218 109 5 124 331 9 220 3.89e-49 164
Msa0084420 MtrunA17_Chr4g0041051 41.146 192 102 2 155 339 64 251 3.96e-32 120
Msa0084420 MtrunA17_Chr2g0325521 42.458 179 92 3 145 316 45 219 1.92e-30 115
Msa0084420 MtrunA17_Chr5g0412371 36.145 166 89 3 157 316 43 197 5.75e-30 113
Msa0084420 MtrunA17_Chr5g0427361 34.934 229 107 4 136 327 8 231 5.76e-27 107
Msa0084420 MtrunA17_Chr4g0045901 43.407 182 89 6 143 317 62 236 9.16e-25 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084420 AT1G68810.1 64.773 264 67 8 96 337 99 358 9.37e-95 286
Msa0084420 AT3G25710.1 50.000 320 98 8 71 333 22 336 1.00e-79 247
Msa0084420 AT3G56770.1 47.059 170 82 4 151 317 37 201 7.31e-43 148
Msa0084420 AT2G41130.1 46.629 178 84 5 143 317 55 224 2.83e-38 137
Msa0084420 AT2G40200.1 42.105 190 89 8 130 311 38 214 1.32e-33 125
Msa0084420 AT4G38070.1 37.324 142 77 3 153 294 87 216 3.19e-25 102
Msa0084420 AT1G01260.1 30.000 180 101 8 38 207 314 478 9.41e-11 63.5
Msa0084420 AT1G01260.2 30.000 180 101 8 38 207 314 478 9.41e-11 63.5
Msa0084420 AT1G01260.3 30.000 180 101 8 38 207 314 478 9.41e-11 63.5

Find 80 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAGAATTGATTAAATTA+TGG 0.270913 1_2:-63842564 Msa0084420:three_prime_UTR
ATTATTATTATATTAGTATA+TGG 0.273119 1_2:-63842511 Msa0084420:three_prime_UTR
ATGCAAGTTTGTTCAATAGA+AGG 0.283417 1_2:-63844368 Msa0084420:CDS
GATGAGTGCTCAAGAAATTA+TGG 0.286315 1_2:-63844220 Msa0084420:CDS
TCAAGACATTGAAAGCATTA+AGG 0.296728 1_2:-63842792 Msa0084420:CDS
TGTTCTAATTGATTCTATAA+TGG 0.302929 1_2:+63845213 None:intergenic
TTCAATGTCTTGATTAGTTC+AGG 0.303436 1_2:+63842802 None:intergenic
ATGCAGGTTCTGAGCATCTT+AGG 0.326044 1_2:-63844314 Msa0084420:CDS
TATACTAATATAATAATAAT+AGG 0.326223 1_2:+63842514 None:intergenic
GTTGTAGATGAATCTGCTTC+TGG 0.328817 1_2:-63842631 Msa0084420:CDS
GCAGATGATGATGAAGATTA+TGG 0.331671 1_2:-63842889 Msa0084420:CDS
AATAGTGATCATCATATCTT+TGG 0.353704 1_2:-63844549 Msa0084420:CDS
TGATCTTCTTGTATCATACT+TGG 0.359086 1_2:+63844660 None:intergenic
ACAATGAGGTAATGATAATT+TGG 0.372247 1_2:-63842339 Msa0084420:three_prime_UTR
GATGAAGATTATGGATCAAA+TGG 0.416803 1_2:-63842880 Msa0084420:CDS
TTCAGCTTCACTATGACTCT+TGG 0.417321 1_2:+63844176 None:intergenic
TTATATTAGTATATGGTACT+TGG 0.421823 1_2:-63842504 Msa0084420:three_prime_UTR
TCATCACATTCAGTTGGAAC+TGG 0.424698 1_2:+63842928 None:intergenic
GTTTGTTTCTCTTGTGGTGG+TGG 0.425567 1_2:+63845264 None:intergenic
TGTTGTTGAGGAATAACAAA+AGG 0.437987 1_2:+63844402 None:intergenic
CCTCACTTTGCTGTGATGAT+AGG 0.438078 1_2:-63842837 Msa0084420:CDS
TTCTTTGTTTGTTTCTCTTG+TGG 0.438449 1_2:+63845258 None:intergenic
GGATCAGGTTCAGGACCCTT+TGG 0.439350 1_2:-63844267 Msa0084420:CDS
CTATTGAACAAACTTGCATA+AGG 0.450006 1_2:+63844372 None:intergenic
ATACTTACTTTGGTTGTGCT+TGG 0.452519 1_2:+63844099 None:intergenic
GAGAGATAAATCTAAAGCCT+TGG 0.462647 1_2:-63845303 Msa0084420:five_prime_UTR
CTTCAACATGGATCAGGTTC+AGG 0.466813 1_2:-63844276 Msa0084420:CDS
AGAGAGCAACAAAGCACAAA+GGG 0.469426 1_2:-63845444 Msa0084420:five_prime_UTR
GCCTCATCATCTGTTGTTGA+AGG 0.470688 1_2:+63844577 None:intergenic
GACCATGTTTGTTGCAAGAC+TGG 0.480750 1_2:+63844474 None:intergenic
TGCAAGTTTGTTCAATAGAA+GGG 0.484018 1_2:-63844367 Msa0084420:CDS
GTAAGATGTTGTTATGTGTA+TGG 0.487450 1_2:-63842384 Msa0084420:three_prime_UTR
CTAATTGATTCTATAATGGT+GGG 0.495009 1_2:+63845217 None:intergenic
GTTAATTCATCACATTCAGT+TGG 0.495856 1_2:+63842922 None:intergenic
TCTAATTGATTCTATAATGG+TGG 0.508231 1_2:+63845216 None:intergenic
ATGAGGTAATGATAATTTGG+TGG 0.510319 1_2:-63842336 Msa0084420:three_prime_UTR
GCAACAAAGCACAAAGGGAA+AGG 0.511848 1_2:-63845439 Msa0084420:five_prime_UTR
TCAGGTTCAGGACCCTTTGG+TGG 0.514868 1_2:-63844264 Msa0084420:CDS
TGATCACCTCCTCCTGCTGT+AGG 0.517327 1_2:+63844507 None:intergenic
TCAAATCTCTTTCACTTGGT+AGG 0.518755 1_2:+63845357 None:intergenic
TCACTACGCACAAATGTTTG+TGG 0.519274 1_2:-63845389 Msa0084420:five_prime_UTR
TTTGTTTGTTTCTCTTGTGG+TGG 0.520406 1_2:+63845261 None:intergenic
AGTGATCATCATATCTTTGG+TGG 0.524209 1_2:-63844546 Msa0084420:CDS
AGGTCTATGTTCCCTACAGC+AGG 0.528893 1_2:-63844519 Msa0084420:CDS
GCTGATATAACAACACTTGG+AGG 0.532615 1_2:-63842754 Msa0084420:CDS
TTTGTTTCTCTTGTGGTGGT+GGG 0.534262 1_2:+63845265 None:intergenic
AAAGGGTCATGAGGATTGAA+AGG 0.537899 1_2:+63844420 None:intergenic
CACCAGTCTTGCAACAAACA+TGG 0.540530 1_2:-63844476 Msa0084420:CDS
GTTGTTGAGGAATAACAAAA+GGG 0.542912 1_2:+63844403 None:intergenic
TTTGCATATGATCATCATGC+AGG 0.543634 1_2:-63844330 Msa0084420:CDS
TCAAGAAATTATGGAGGCTA+AGG 0.546278 1_2:-63844211 Msa0084420:CDS
GATCACCTCCTCCTGCTGTA+GGG 0.548133 1_2:+63844508 None:intergenic
AATTGATTCTATAATGGTGG+GGG 0.552128 1_2:+63845219 None:intergenic
GATACACTTCAACATGGATC+AGG 0.552437 1_2:-63844282 Msa0084420:CDS
TAATTGATTCTATAATGGTG+GGG 0.553540 1_2:+63845218 None:intergenic
ATCATATCTTTGGTGGAGGA+AGG 0.562826 1_2:-63844539 Msa0084420:CDS
TCATATGCAAACTGAAGAGA+AGG 0.572922 1_2:+63844342 None:intergenic
AGAAGCACTCAAAGCTGTGA+TGG 0.573378 1_2:-63842662 Msa0084420:CDS
AGCTGCGTAGCTTAGCAAGA+TGG 0.576612 1_2:+63844127 None:intergenic
TCCTTCAACAACAGATGATG+AGG 0.577140 1_2:-63844578 Msa0084420:CDS
GATCATCATATCTTTGGTGG+AGG 0.579767 1_2:-63844543 Msa0084420:CDS
ATGTTCCCTACAGCAGGAGG+AGG 0.581224 1_2:-63844513 Msa0084420:CDS
TTCACCTTGAAAACCACCAA+AGG 0.584286 1_2:+63844251 None:intergenic
TCACCTTGAAAACCACCAAA+GGG 0.586225 1_2:+63844252 None:intergenic
AATGTCAAATCTCTTTCACT+TGG 0.587124 1_2:+63845353 None:intergenic
TAAGGTGAAGCTTGTTGTTG+AGG 0.593376 1_2:+63844390 None:intergenic
ATTAGAACAATATCTATCAA+AGG 0.593991 1_2:-63845199 Msa0084420:intron
AAGAGAGCAACAAAGCACAA+AGG 0.595215 1_2:-63845445 Msa0084420:five_prime_UTR
AGAGTCATAGTGAAGCTGAA+AGG 0.601072 1_2:-63844173 Msa0084420:CDS
GTTTCTCTTGTGGTGGTGGG+CGG 0.602754 1_2:+63845268 None:intergenic
AAAGCTGATATAACAACACT+TGG 0.602897 1_2:-63842757 Msa0084420:CDS
CTGGAAGTGTCAAGAGACAA+AGG 0.603991 1_2:-63842612 Msa0084420:CDS
GGAATAACAAAAGGGTCATG+AGG 0.626831 1_2:+63844411 None:intergenic
CCTATCATCACAGCAAAGTG+AGG 0.634985 1_2:+63842837 None:intergenic
AGTATGATACAAGAAGATCA+AGG 0.636259 1_2:-63844657 Msa0084420:CDS
TCTATGTTCCCTACAGCAGG+AGG 0.647545 1_2:-63844516 Msa0084420:CDS
GGCGGCCAAAGAAAAGTCCA+AGG 0.663085 1_2:+63845286 None:intergenic
ATATCAGATACACTTCAACA+TGG 0.699194 1_2:-63844288 Msa0084420:CDS
GAGTGCTCAAGAAATTATGG+AGG 0.703562 1_2:-63844217 Msa0084420:CDS
AACATGATGATGAGACAATG+AGG 0.726295 1_2:-63842353 Msa0084420:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATATTAGTACTATAT+TGG + chr1_2:63843836-63843855 None:intergenic 10.0%
!! AAATTTATAGTTAATTAAAG+TGG + chr1_2:63844655-63844674 None:intergenic 10.0%
!! AATTAGAATTGATTAAATTA+TGG - chr1_2:63845208-63845227 Msa0084420:five_prime_UTR 10.0%
!!! TTTTTATCTTTTTTTTTTTG+GGG - chr1_2:63845323-63845342 Msa0084420:five_prime_UTR 10.0%
!! ATATATACATATGAATATGA+TGG + chr1_2:63843045-63843064 None:intergenic 15.0%
!! ATGTATATATATATAAACCT+TGG - chr1_2:63843055-63843074 Msa0084420:intron 15.0%
!!! TTTTATCTTTTTTTTTTTGG+GGG - chr1_2:63845324-63845343 Msa0084420:five_prime_UTR 15.0%
!!! TGTTCTAATTGATTCTATAA+TGG + chr1_2:63842562-63842581 None:intergenic 20.0%
!! ATTAGAACAATATCTATCAA+AGG - chr1_2:63842573-63842592 Msa0084420:exon 20.0%
!! ACAAGGAATAAAAATGAAAA+GGG + chr1_2:63842668-63842687 None:intergenic 20.0%
!!! TAAGCAAAAATCTACTTTTT+AGG - chr1_2:63842811-63842830 Msa0084420:CDS 20.0%
!! CATTTAATAAATGATGTATG+TGG + chr1_2:63842875-63842894 None:intergenic 20.0%
!! ATATATATATATACATCCTC+CGG + chr1_2:63842932-63842951 None:intergenic 20.0%
!! TTACTACTATATACTTACTT+TGG + chr1_2:63843686-63843705 None:intergenic 20.0%
!!! AGATATTTTGAATCTACAAA+AGG + chr1_2:63843942-63843961 None:intergenic 20.0%
!!! GATTTTGATTTTGTTGTTTA+TGG + chr1_2:63843977-63843996 None:intergenic 20.0%
!!! ATATAACCTTTTAGAAAATG+AGG - chr1_2:63843999-63844018 Msa0084420:intron 20.0%
!!! TGTAACTATATGATTTGTTT+TGG - chr1_2:63844134-63844153 Msa0084420:CDS 20.0%
!!! GTAACTATATGATTTGTTTT+GGG - chr1_2:63844135-63844154 Msa0084420:CDS 20.0%
!!! AATAGTGGATATATTTTGAA+TGG + chr1_2:63844442-63844461 None:intergenic 20.0%
!! TATGATATAAGAATGTGAAA+TGG + chr1_2:63844466-63844485 None:intergenic 20.0%
!! ATAGATCTGATATATACATA+AGG + chr1_2:63844507-63844526 None:intergenic 20.0%
!! TGAAAATATTTACAATCTCA+TGG + chr1_2:63844540-63844559 None:intergenic 20.0%
!! TTATATTAGTATATGGTACT+TGG - chr1_2:63845268-63845287 Msa0084420:five_prime_UTR 20.0%
!!! TCTTTTCTTTTCTTTTTTCA+TGG - chr1_2:63845359-63845378 Msa0084420:five_prime_UTR 20.0%
!! TAATTGATTCTATAATGGTG+GGG + chr1_2:63842557-63842576 None:intergenic 25.0%
!! CTAATTGATTCTATAATGGT+GGG + chr1_2:63842558-63842577 None:intergenic 25.0%
!! TCTAATTGATTCTATAATGG+TGG + chr1_2:63842559-63842578 None:intergenic 25.0%
!!! AAATGAAAAGGGTATTTGAA+AGG + chr1_2:63842657-63842676 None:intergenic 25.0%
! CACAAGGAATAAAAATGAAA+AGG + chr1_2:63842669-63842688 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr1_2:63842801-63842820 None:intergenic 25.0%
! CATTCAATAAATGATGTATG+TGG - chr1_2:63842836-63842855 Msa0084420:CDS 25.0%
!! ATTTTGCTTATAATAGTGAC+CGG - chr1_2:63842910-63842929 Msa0084420:CDS 25.0%
! ACAAAATAAAACCTAGCTAT+AGG - chr1_2:63842988-63843007 Msa0084420:CDS 25.0%
! TATATATAAACCTTGGAGTT+TGG - chr1_2:63843062-63843081 Msa0084420:intron 25.0%
! AATAGTGATCATCATATCTT+TGG - chr1_2:63843223-63843242 Msa0084420:intron 25.0%
!! GGTTTGCATTTTAAATTTCT+TGG + chr1_2:63843792-63843811 None:intergenic 25.0%
! GGCATATATTGAATTCAAAA+AGG + chr1_2:63843921-63843940 None:intergenic 25.0%
! TGAAAATATATTGTCTTTGC+CGG - chr1_2:63844299-63844318 Msa0084420:CDS 25.0%
! TGGAGAATGAAAAGAAATAA+AGG - chr1_2:63844349-63844368 Msa0084420:CDS 25.0%
! ATCTGATATATACATAAGGT+TGG + chr1_2:63844503-63844522 None:intergenic 25.0%
! ATATTTACAATCTCATGGTA+GGG + chr1_2:63844535-63844554 None:intergenic 25.0%
! AATATTTACAATCTCATGGT+AGG + chr1_2:63844536-63844555 None:intergenic 25.0%
!!! AAGAATGTTTTGTTCATTAC+TGG - chr1_2:63845048-63845067 Msa0084420:intron 25.0%
! ACAATGAGGTAATGATAATT+TGG - chr1_2:63845433-63845452 Msa0084420:five_prime_UTR 25.0%
!!! GTTTTATTTTTACATGTGTG+TGG - chr1_2:63845458-63845477 Msa0084420:five_prime_UTR 25.0%
AATGTCAAATCTCTTTCACT+TGG + chr1_2:63842422-63842441 None:intergenic 30.0%
! TTCTTTGTTTGTTTCTCTTG+TGG + chr1_2:63842517-63842536 None:intergenic 30.0%
! AATTGATTCTATAATGGTGG+GGG + chr1_2:63842556-63842575 None:intergenic 30.0%
GGTATTTGAAAGGACAAAAT+GGG + chr1_2:63842647-63842666 None:intergenic 30.0%
! TGTTTTATATCCAAACTCCA+AGG + chr1_2:63843075-63843094 None:intergenic 30.0%
TTGGAGTTTGGATATAAAAC+AGG - chr1_2:63843074-63843093 Msa0084420:intron 30.0%
TGATCTTCTTGTATCATACT+TGG + chr1_2:63843115-63843134 None:intergenic 30.0%
AGTATGATACAAGAAGATCA+AGG - chr1_2:63843115-63843134 Msa0084420:intron 30.0%
GTTGTTGAGGAATAACAAAA+GGG + chr1_2:63843372-63843391 None:intergenic 30.0%
TGTTGTTGAGGAATAACAAA+AGG + chr1_2:63843373-63843392 None:intergenic 30.0%
CTATTGAACAAACTTGCATA+AGG + chr1_2:63843403-63843422 None:intergenic 30.0%
! ATGCAAGTTTGTTCAATAGA+AGG - chr1_2:63843404-63843423 Msa0084420:intron 30.0%
! TGCAAGTTTGTTCAATAGAA+GGG - chr1_2:63843405-63843424 Msa0084420:intron 30.0%
ATATCAGATACACTTCAACA+TGG - chr1_2:63843484-63843503 Msa0084420:intron 30.0%
!! TTGAGTTGAGATCTTTGATT+AGG + chr1_2:63843813-63843832 None:intergenic 30.0%
! TTTGCACCTCATTTTCTAAA+AGG + chr1_2:63844008-63844027 None:intergenic 30.0%
! GCTTTTGTTAACATCCTTTT+TGG - chr1_2:63844047-63844066 Msa0084420:intron 30.0%
GTATACAAAACAAGCCAAAA+AGG + chr1_2:63844064-63844083 None:intergenic 30.0%
GAATTAGTGTTAATGTCTCT+TGG - chr1_2:63844230-63844249 Msa0084420:CDS 30.0%
TGACTGATCAACATATTATC+CGG + chr1_2:63844321-63844340 None:intergenic 30.0%
!! AGTGGATATATTTTGAATGG+TGG + chr1_2:63844439-63844458 None:intergenic 30.0%
AGAATGTGAAATGGAAATAG+TGG + chr1_2:63844457-63844476 None:intergenic 30.0%
! GATATATACATAAGGTTGGT+AGG + chr1_2:63844499-63844518 None:intergenic 30.0%
GTTAATTCATCACATTCAGT+TGG + chr1_2:63844853-63844872 None:intergenic 30.0%
GATGAAGATTATGGATCAAA+TGG - chr1_2:63844892-63844911 Msa0084420:intron 30.0%
! TTCAATGTCTTGATTAGTTC+AGG + chr1_2:63844973-63844992 None:intergenic 30.0%
!! TCAAGACATTGAAAGCATTA+AGG - chr1_2:63844980-63844999 Msa0084420:intron 30.0%
AAAGCTGATATAACAACACT+TGG - chr1_2:63845015-63845034 Msa0084420:intron 30.0%
!!! TATCTTTTTTTTTTTGGGGG+TGG - chr1_2:63845327-63845346 Msa0084420:five_prime_UTR 30.0%
GTAAGATGTTGTTATGTGTA+TGG - chr1_2:63845388-63845407 Msa0084420:five_prime_UTR 30.0%
ATGAGGTAATGATAATTTGG+TGG - chr1_2:63845436-63845455 Msa0084420:five_prime_UTR 30.0%
TCAAATCTCTTTCACTTGGT+AGG + chr1_2:63842418-63842437 None:intergenic 35.0%
! GAGAGATAAATCTAAAGCCT+TGG - chr1_2:63842469-63842488 Msa0084420:three_prime_UTR 35.0%
! TAAAGCCTTGGACTTTTCTT+TGG - chr1_2:63842481-63842500 Msa0084420:three_prime_UTR 35.0%
! TTTGTTTGTTTCTCTTGTGG+TGG + chr1_2:63842514-63842533 None:intergenic 35.0%
GGGTATTTGAAAGGACAAAA+TGG + chr1_2:63842648-63842667 None:intergenic 35.0%
TATAGGCACTTAATTGCACA+AGG + chr1_2:63842685-63842704 None:intergenic 35.0%
GTCATAATATATACACCCTC+CGG - chr1_2:63842779-63842798 Msa0084420:CDS 35.0%
AAATGTCAGAACCTATAGCT+AGG + chr1_2:63843002-63843021 None:intergenic 35.0%
AGTGATCATCATATCTTTGG+TGG - chr1_2:63843226-63843245 Msa0084420:intron 35.0%
TCATATGCAAACTGAAGAGA+AGG + chr1_2:63843433-63843452 None:intergenic 35.0%
TTTGCATATGATCATCATGC+AGG - chr1_2:63843442-63843461 Msa0084420:intron 35.0%
GATGAGTGCTCAAGAAATTA+TGG - chr1_2:63843552-63843571 Msa0084420:intron 35.0%
TCAAGAAATTATGGAGGCTA+AGG - chr1_2:63843561-63843580 Msa0084420:intron 35.0%
ATACTTACTTTGGTTGTGCT+TGG + chr1_2:63843676-63843695 None:intergenic 35.0%
!!! GCAAGAGTGTTTTTTCTAAG+TGG - chr1_2:63843895-63843914 Msa0084420:intron 35.0%
TATAATGTGTGGTAGTTCCA+AGG - chr1_2:63844166-63844185 Msa0084420:CDS 35.0%
TGTCTCTTGGTATGATAAAG+AGG - chr1_2:63844243-63844262 Msa0084420:CDS 35.0%
!! TTTTCAACTGCATGCTGTTT+TGG + chr1_2:63844271-63844290 None:intergenic 35.0%
GTTGATCAGTCAAACTCAAT+TGG - chr1_2:63844329-63844348 Msa0084420:CDS 35.0%
GTTATGTTTGTGACTGACAA+AGG + chr1_2:63844393-63844412 None:intergenic 35.0%
! GCAGATGATGATGAAGATTA+TGG - chr1_2:63844883-63844902 Msa0084420:intron 35.0%
! AACATGATGATGAGACAATG+AGG - chr1_2:63845419-63845438 Msa0084420:five_prime_UTR 35.0%
!! GTGCTTTGTTGCTCTCTTTT+TGG + chr1_2:63842325-63842344 None:intergenic 40.0%
AAGAGAGCAACAAAGCACAA+AGG - chr1_2:63842327-63842346 Msa0084420:three_prime_UTR 40.0%
AGAGAGCAACAAAGCACAAA+GGG - chr1_2:63842328-63842347 Msa0084420:three_prime_UTR 40.0%
TCACTACGCACAAATGTTTG+TGG - chr1_2:63842383-63842402 Msa0084420:three_prime_UTR 40.0%
! TTTGTTTCTCTTGTGGTGGT+GGG + chr1_2:63842510-63842529 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr1_2:63842914-63842933 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr1_2:63842913-63842932 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr1_2:63842913-63842932 Msa0084420:CDS 40.0%
TCCTTCAACAACAGATGATG+AGG - chr1_2:63843194-63843213 Msa0084420:intron 40.0%
GATCATCATATCTTTGGTGG+AGG - chr1_2:63843229-63843248 Msa0084420:intron 40.0%
ATCATATCTTTGGTGGAGGA+AGG - chr1_2:63843233-63843252 Msa0084420:intron 40.0%
AAAGGGTCATGAGGATTGAA+AGG + chr1_2:63843355-63843374 None:intergenic 40.0%
GGAATAACAAAAGGGTCATG+AGG + chr1_2:63843364-63843383 None:intergenic 40.0%
!! TAAGGTGAAGCTTGTTGTTG+AGG + chr1_2:63843385-63843404 None:intergenic 40.0%
GATACACTTCAACATGGATC+AGG - chr1_2:63843490-63843509 Msa0084420:intron 40.0%
TCACCTTGAAAACCACCAAA+GGG + chr1_2:63843523-63843542 None:intergenic 40.0%
TTCACCTTGAAAACCACCAA+AGG + chr1_2:63843524-63843543 None:intergenic 40.0%
GAGTGCTCAAGAAATTATGG+AGG - chr1_2:63843555-63843574 Msa0084420:intron 40.0%
TTCAGCTTCACTATGACTCT+TGG + chr1_2:63843599-63843618 None:intergenic 40.0%
AGAGTCATAGTGAAGCTGAA+AGG - chr1_2:63843599-63843618 Msa0084420:intron 40.0%
CTAGCTGGCTAAAGAAAAAG+TGG - chr1_2:63844195-63844214 Msa0084420:CDS 40.0%
TCATCACATTCAGTTGGAAC+TGG + chr1_2:63844847-63844866 None:intergenic 40.0%
GCTGATATAACAACACTTGG+AGG - chr1_2:63845018-63845037 Msa0084420:intron 40.0%
GTTGTAGATGAATCTGCTTC+TGG - chr1_2:63845141-63845160 Msa0084420:intron 40.0%
GCAACAAAGCACAAAGGGAA+AGG - chr1_2:63842333-63842352 Msa0084420:three_prime_UTR 45.0%
! GTTTGTTTCTCTTGTGGTGG+TGG + chr1_2:63842511-63842530 None:intergenic 45.0%
GCCTCATCATCTGTTGTTGA+AGG + chr1_2:63843198-63843217 None:intergenic 45.0%
CACCAGTCTTGCAACAAACA+TGG - chr1_2:63843296-63843315 Msa0084420:intron 45.0%
! GACCATGTTTGTTGCAAGAC+TGG + chr1_2:63843301-63843320 None:intergenic 45.0%
!! ATGCAGGTTCTGAGCATCTT+AGG - chr1_2:63843458-63843477 Msa0084420:intron 45.0%
CTTCAACATGGATCAGGTTC+AGG - chr1_2:63843496-63843515 Msa0084420:intron 45.0%
!! GGTGGTTTTCAAGGTGAAGT+TGG - chr1_2:63843526-63843545 Msa0084420:intron 45.0%
!! GGGAGTGTCTCTATAATGTG+TGG - chr1_2:63844155-63844174 Msa0084420:CDS 45.0%
CCTATCATCACAGCAAAGTG+AGG + chr1_2:63844938-63844957 None:intergenic 45.0%
! CCTCACTTTGCTGTGATGAT+AGG - chr1_2:63844935-63844954 Msa0084420:intron 45.0%
!! AGAAGCACTCAAAGCTGTGA+TGG - chr1_2:63845110-63845129 Msa0084420:intron 45.0%
!! CTGGAAGTGTCAAGAGACAA+AGG - chr1_2:63845160-63845179 Msa0084420:intron 45.0%
!! AATAAAAAAAAAATCTATAT+AGG + chr1_2:63842702-63842721 None:intergenic 5.0%
!!! TATAAATTTTATCTAATTAA+AGG - chr1_2:63845235-63845254 Msa0084420:five_prime_UTR 5.0%
!! TATACTAATATAATAATAAT+AGG + chr1_2:63845261-63845280 None:intergenic 5.0%
!! ATTATTATTATATTAGTATA+TGG - chr1_2:63845261-63845280 Msa0084420:five_prime_UTR 5.0%
!!! GTTATTATATATTTTTTATT+AGG - chr1_2:63845290-63845309 Msa0084420:five_prime_UTR 5.0%
!!! AATTTTTATCTTTTTTTTTT+TGG - chr1_2:63845321-63845340 Msa0084420:five_prime_UTR 5.0%
!!! ATTTTTATCTTTTTTTTTTT+GGG - chr1_2:63845322-63845341 Msa0084420:five_prime_UTR 5.0%
AGGTCTATGTTCCCTACAGC+AGG - chr1_2:63843253-63843272 Msa0084420:intron 50.0%
TCTATGTTCCCTACAGCAGG+AGG - chr1_2:63843256-63843275 Msa0084420:intron 50.0%
!! GGACCCTTTGGTGGTTTTCA+AGG - chr1_2:63843517-63843536 Msa0084420:intron 50.0%
AGCTGCGTAGCTTAGCAAGA+TGG + chr1_2:63843648-63843667 None:intergenic 50.0%
TGAGCTGAGCTGAGATCTCA+TGG + chr1_2:63844566-63844585 None:intergenic 50.0%
GGCGGCCAAAGAAAAGTCCA+AGG + chr1_2:63842489-63842508 None:intergenic 55.0%
!! GTTTCTCTTGTGGTGGTGGG+CGG + chr1_2:63842507-63842526 None:intergenic 55.0%
ATGTTCCCTACAGCAGGAGG+AGG - chr1_2:63843259-63843278 Msa0084420:intron 55.0%
GATCACCTCCTCCTGCTGTA+GGG + chr1_2:63843267-63843286 None:intergenic 55.0%
TGATCACCTCCTCCTGCTGT+AGG + chr1_2:63843268-63843287 None:intergenic 55.0%
GGATCAGGTTCAGGACCCTT+TGG - chr1_2:63843505-63843524 Msa0084420:intron 55.0%
TCAGGTTCAGGACCCTTTGG+TGG - chr1_2:63843508-63843527 Msa0084420:intron 55.0%
GTTCCAAGGAAGCAGCTAGC+TGG - chr1_2:63844180-63844199 Msa0084420:CDS 55.0%
TAGCCAGCTAGCTGCTTCCT+TGG + chr1_2:63844186-63844205 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 63842307 63845487 63842307 ID=Msa0084420;Name=Msa0084420
chr1_2 mRNA 63842307 63845487 63842307 ID=Msa0084420-mRNA-1;Parent=Msa0084420;Name=Msa0084420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=310|1|1|1|0.5|0.33|3|278|339
chr1_2 exon 63842307 63843034 63842307 ID=Msa0084420-mRNA-1:exon:21428;Parent=Msa0084420-mRNA-1
chr1_2 exon 63844107 63844698 63844107 ID=Msa0084420-mRNA-1:exon:21427;Parent=Msa0084420-mRNA-1
chr1_2 exon 63845200 63845487 63845200 ID=Msa0084420-mRNA-1:exon:21426;Parent=Msa0084420-mRNA-1
chr1_2 five_prime_UTR 63845200 63845487 63845200 ID=Msa0084420-mRNA-1:five_prime_utr;Parent=Msa0084420-mRNA-1
chr1_2 five_prime_UTR 63844677 63844698 63844677 ID=Msa0084420-mRNA-1:five_prime_utr;Parent=Msa0084420-mRNA-1
chr1_2 CDS 63844107 63844676 63844107 ID=Msa0084420-mRNA-1:cds;Parent=Msa0084420-mRNA-1
chr1_2 CDS 63842585 63843034 63842585 ID=Msa0084420-mRNA-1:cds;Parent=Msa0084420-mRNA-1
chr1_2 three_prime_UTR 63842307 63842584 63842307 ID=Msa0084420-mRNA-1:three_prime_utr;Parent=Msa0084420-mRNA-1
Gene Sequence

>Msa0084420

ATGATACAAGAAGATCAAGGACAGTGTTCATCTCAAACAATCAATAACTTTGAAACTTATCAACAAGAGCAGTTTCTCCTTCAACAACAGATGATGAGGCAACAAAATAGTGATCATCATATCTTTGGTGGAGGAAGGTCTATGTTCCCTACAGCAGGAGGAGGTGATCATCAAGTTTCACCAGTCTTGCAACAAACATGGTCTATGCAACAACTTCATCATCATCATCATCATCCTTTCAATCCTCATGACCCTTTTGTTATTCCTCAACAACAAGCTTCACCTTATGCAAGTTTGTTCAATAGAAGGGTTCCTTCTCTTCAGTTTGCATATGATCATCATGCAGGTTCTGAGCATCTTAGGATCATATCAGATACACTTCAACATGGATCAGGTTCAGGACCCTTTGGTGGTTTTCAAGGTGAAGTTGGAAAGATGAGTGCTCAAGAAATTATGGAGGCTAAGGCTCTTGCTGCTTCCAAGAGTCATAGTGAAGCTGAAAGGAGACGCAGAGAGAGAATCAATAACCATCTTGCTAAGCTACGCAGCTTGTTGCCAAGCACAACCAAAACAGACAAAGCATCATTGCTAGCAGAAGTGATACAACATGTAAAAGAATTGAAGCGTCAAACATCGTTGATAGCAGAAACAAGTCCAGTTCCAACTGAATGTGATGAATTAACAGTAGATGCTGCAGATGATGATGAAGATTATGGATCAAATGGAAACAAGTTTATCATAAAAGCCTCACTTTGCTGTGATGATAGGTCAGATCTTTTGCCTGAACTAATCAAGACATTGAAAGCATTAAGGTTAAGAACACTCAAAGCTGATATAACAACACTTGGAGGACGTGTTAAGAATGTTTTGTTCATTACTGGTGAAGAAGATGATCATGAATATTGCATTAGTTCAATACAAGAAGCACTCAAAGCTGTGATGGAGAAGAGTGTTGTAGATGAATCTGCTTCTGGAAGTGTCAAGAGACAAAGGACTAATATAATATCAATATCTAATTAA
Protein sequence

>Msa0084420

MIQEDQGQCSSQTINNFETYQQEQFLLQQQMMRQQNSDHHIFGGGRSMFPTAGGGDHQVSPVLQQTWSMQQLHHHHHHPFNPHDPFVIPQQQASPYASLFNRRVPSLQFAYDHHAGSEHLRIISDTLQHGSGSGPFGGFQGEVGKMSAQEIMEAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPVPTECDELTVDAADDDEDYGSNGNKFIIKASLCCDDRSDLLPELIKTLKALRLRTLKADITTLGGRVKNVLFITGEEDDHEYCISSIQEALKAVMEKSVVDESASGSVKRQRTNIISISN*