AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0182760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0182760 MtrunA17_Chr1g0209981 97.664 214 5 0 2 215 1 214 2.18e-158 436
Msa0182760 MtrunA17_Chr2g0292061 46.766 201 103 1 15 215 79 275 2.96e-62 195
Msa0182760 MtrunA17_Chr2g0292701 38.308 201 118 1 15 215 64 258 3.12e-50 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0182760 AT4G39670.1 66.010 203 68 1 14 215 27 229 1.59e-99 288
Msa0182760 AT2G34690.1 44.828 203 106 2 14 215 9 206 1.34e-58 184

Find 67 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCGGCTATTTGGAGATTT+AGG 0.095751 1_4:+71594332 Msa0182760:three_prime_UTR
CTGTATATACATAATCTATT+TGG 0.187363 1_4:+71594423 Msa0182760:three_prime_UTR
GAAAGAGCAATACTGAAATT+TGG 0.217120 1_4:+71594476 Msa0182760:three_prime_UTR
TCAGTTTCTCAGCGGCTATT+TGG 0.268239 1_4:+71594323 Msa0182760:three_prime_UTR
AGCGGCTATTTGGAGATTTA+GGG 0.286497 1_4:+71594333 Msa0182760:three_prime_UTR
TGCTTTCAAATTCGCTGAAA+TGG 0.298390 1_4:+71592185 Msa0182760:CDS
TTATCAATGTATTCTATAAC+TGG 0.304225 1_4:-71594174 None:intergenic
GGATCTTTCCACAAAGGTTC+AGG 0.305144 1_4:+71592068 Msa0182760:CDS
CTTGATTCCCTGAACCTTTG+TGG 0.341847 1_4:-71592076 None:intergenic
TGCCATTGCCGAAGCCTTTG+AGG 0.347970 1_4:+71592047 Msa0182760:CDS
AGGATACGCAATCGCTAAAT+CGG 0.356164 1_4:-71592019 None:intergenic
TCATACACACCCGTATTCAT+AGG 0.367979 1_4:+71591785 Msa0182760:five_prime_UTR
TTTGTAAAGCTCAATGAAAC+AGG 0.368946 1_4:+71593388 Msa0182760:CDS
AAGAAAGAAAGAGAGTGAAT+AGG 0.376034 1_4:-71591824 None:intergenic
GAAGTGGCGTCAACAGCTTA+TGG 0.379855 1_4:+71593280 Msa0182760:CDS
CCTTTCTCGAAATATTTCAT+TGG 0.382644 1_4:+71594203 Msa0182760:CDS
ATTTCAGTATTGCTCTTTCA+AGG 0.393384 1_4:-71594472 None:intergenic
AAAACAAACGATGGTTGTAA+TGG 0.401175 1_4:+71591990 Msa0182760:exon
CTCGAAATATTTCATTGGAC+TGG 0.428539 1_4:+71594208 Msa0182760:CDS
TAATCTACGCAGAGTTCGCC+AGG 0.430093 1_4:+71592949 Msa0182760:CDS
GTCAGAACGGCTGTGTATGC+TGG 0.431196 1_4:+71593334 Msa0182760:CDS
AGTGAATAGGAAGGGAAGGA+AGG 0.448997 1_4:-71591811 None:intergenic
CGTGACACAGTGAAAACATC+TGG 0.453856 1_4:+71592914 Msa0182760:CDS
AGGAAGGTTCCCTATGAATA+CGG 0.461002 1_4:-71591795 None:intergenic
AGAGAGTGAATAGGAAGGGA+AGG 0.473097 1_4:-71591815 None:intergenic
TGTTGTAGTGGCGATCTGTT+CGG 0.477164 1_4:+71594291 Msa0182760:three_prime_UTR
CCAATGAAATATTTCGAGAA+AGG 0.487284 1_4:-71594203 None:intergenic
AAGAAAGAGAGTGAATAGGA+AGG 0.493438 1_4:-71591820 None:intergenic
GGAAAGATCCTCAAAGGCTT+CGG 0.500738 1_4:-71592055 None:intergenic
TATGAATACGGGTGTGTATG+AGG 0.504151 1_4:-71591783 None:intergenic
GATTGGTATGCTCAGCTACA+TGG 0.504762 1_4:+71592830 Msa0182760:intron
TTTGTGCACCGTATCACTCA+TGG 0.508447 1_4:+71593308 Msa0182760:CDS
TATATACATAATCTATTTGG+AGG 0.509298 1_4:+71594426 Msa0182760:three_prime_UTR
GATCTTTCCACAAAGGTTCA+GGG 0.519873 1_4:+71592069 Msa0182760:CDS
AGAAAGAGAGTGAATAGGAA+GGG 0.521269 1_4:-71591819 None:intergenic
CTGTGTCACGGGCCACATCA+TGG 0.522770 1_4:-71592901 None:intergenic
TTGTGCACCGTATCACTCAT+GGG 0.532403 1_4:+71593309 Msa0182760:CDS
GAGGCAGTGATGTGCTCCAT+TGG 0.532940 1_4:+71594243 Msa0182760:three_prime_UTR
ATCCTCAAAGGCTTCGGCAA+TGG 0.535128 1_4:-71592049 None:intergenic
TTTCAGTATTGCTCTTTCAA+GGG 0.538267 1_4:-71594471 None:intergenic
CTGACTCCCCATGAGTGATA+CGG 0.553379 1_4:-71593316 None:intergenic
CAGCGGAGAAGAAGATGAGA+AGG 0.559369 1_4:+71594133 Msa0182760:CDS
CATACACACCCGTATTCATA+GGG 0.563694 1_4:+71591786 Msa0182760:five_prime_UTR
GGAAGGTTCCCTATGAATAC+GGG 0.573432 1_4:-71591794 None:intergenic
GCTTCGGCAATGGCAGAAAG+AGG 0.573499 1_4:-71592039 None:intergenic
CTCTAACGAGATCAAGACCC+TGG 0.574080 1_4:-71592967 None:intergenic
CTTTGAGGATCTTTCCACAA+AGG 0.574860 1_4:+71592062 Msa0182760:CDS
ATAAAGAAAATGTCAACCGC+TGG 0.576083 1_4:-71591740 None:intergenic
CTTTGTGGAAAGATCCTCAA+AGG 0.582564 1_4:-71592061 None:intergenic
TCACTCATGGGGAGTCAGAA+CGG 0.583291 1_4:+71593321 Msa0182760:CDS
TGCTCAGCTACATGGACTGG+TGG 0.589627 1_4:+71592838 Msa0182760:intron
GTATGCTCAGCTACATGGAC+TGG 0.590660 1_4:+71592835 Msa0182760:intron
GGAGTCTTACGATGAAATGT+CGG 0.605581 1_4:-71591902 None:intergenic
GCATTGTACTGCTATACCAA+TGG 0.613273 1_4:-71594259 None:intergenic
TGATATTTATCTGAAAGAAG+TGG 0.618563 1_4:+71593264 Msa0182760:CDS
GTCTAAGCGAATATGTTGAG+TGG 0.619668 1_4:-71592109 None:intergenic
TGAAATGGAATACGTTGCCA+AGG 0.620132 1_4:+71592200 Msa0182760:CDS
GTATTCTATAACTGGACGTG+AGG 0.634779 1_4:-71594166 None:intergenic
AAGGTTCAGGGAATCAAGAA+CGG 0.645785 1_4:+71592081 Msa0182760:CDS
GGCGTCAACAGCTTATGGAG+AGG 0.646741 1_4:+71593285 Msa0182760:CDS
TAAGCGAATATGTTGAGTGG+AGG 0.653086 1_4:-71592106 None:intergenic
TGTGCACCGTATCACTCATG+GGG 0.681218 1_4:+71593310 Msa0182760:CDS
TTAATTAGTCAGTTTCTCAG+CGG 0.684824 1_4:+71594315 Msa0182760:three_prime_UTR
TGTTATTGCAGAGCAATCAG+CGG 0.688575 1_4:+71594116 Msa0182760:intron
AGATGTAATTGACCATGATG+TGG 0.712076 1_4:+71592889 Msa0182760:CDS
AATCTACGCAGAGTTCGCCA+GGG 0.712951 1_4:+71592950 Msa0182760:CDS
GGACTGGTGAGTTCTAGCAG+AGG 0.732675 1_4:+71594224 Msa0182760:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATTTTTGATACATTAA+GGG - chr1_4:71593526-71593545 None:intergenic 10.0%
!!! TATAAATTTTTGATACATTA+AGG - chr1_4:71593527-71593546 None:intergenic 10.0%
!! AAAAAAAGAACAAAAAAATT+AGG + chr1_4:71594362-71594381 Msa0182760:three_prime_UTR 10.0%
!!! ATTGAAAAATAATTAGTTTC+TGG - chr1_4:71591856-71591875 None:intergenic 15.0%
!!! TATTAGTTTATCATATTATC+TGG + chr1_4:71592777-71592796 Msa0182760:intron 15.0%
!!! ATTAGTTTATCATATTATCT+GGG + chr1_4:71592778-71592797 Msa0182760:intron 15.0%
!!! TTTGTGATTTCTTTTTATGA+AGG - chr1_4:71591952-71591971 None:intergenic 20.0%
!! ATAATTTGACATTTGATACA+AGG + chr1_4:71592330-71592349 Msa0182760:intron 20.0%
!!! AAAAATATTGTGAGAGTTTT+AGG + chr1_4:71592624-71592643 Msa0182760:intron 20.0%
!! AAAGTGATACATGTATAAAT+AGG - chr1_4:71593029-71593048 None:intergenic 20.0%
!!! TTGTTTTGTTTTGTTATGTT+CGG + chr1_4:71593420-71593439 Msa0182760:intron 20.0%
!!! GTCTTATGTTACTAATTTTA+TGG + chr1_4:71593562-71593581 Msa0182760:intron 20.0%
!!! ACTCTATAATGCTTTTATAT+TGG + chr1_4:71593728-71593747 Msa0182760:intron 20.0%
!! AAATAAACAGACTAATGAAA+GGG - chr1_4:71593922-71593941 None:intergenic 20.0%
!! AAAATAAACAGACTAATGAA+AGG - chr1_4:71593923-71593942 None:intergenic 20.0%
!! ACAACACAAAGATTTATATA+AGG - chr1_4:71593967-71593986 None:intergenic 20.0%
!!! ATTATTTCTATGTAAGATGA+AGG - chr1_4:71594084-71594103 None:intergenic 20.0%
!! TTATCAATGTATTCTATAAC+TGG - chr1_4:71594177-71594196 None:intergenic 20.0%
!!! AAATTAGGATTTTGCTAAAA+CGG + chr1_4:71594377-71594396 Msa0182760:three_prime_UTR 20.0%
!! CTGTATATACATAATCTATT+TGG + chr1_4:71594423-71594442 Msa0182760:three_prime_UTR 20.0%
!! TATATACATAATCTATTTGG+AGG + chr1_4:71594426-71594445 Msa0182760:three_prime_UTR 20.0%
!!! TTGATGTTTTTTATTACCAG+CGG + chr1_4:71591724-71591743 Msa0182760:five_prime_UTR 25.0%
!!! AGTGATTTTAAAACAAACGA+TGG + chr1_4:71591981-71592000 Msa0182760:exon 25.0%
! GTGTAAAACATTACATACAT+TGG - chr1_4:71592533-71592552 None:intergenic 25.0%
! TGATGTGAATATATGATGAT+TGG + chr1_4:71592813-71592832 Msa0182760:intron 25.0%
! TGATATTTATCTGAAAGAAG+TGG + chr1_4:71593264-71593283 Msa0182760:CDS 25.0%
! ACTAATAGAATACACATTTG+AGG + chr1_4:71593631-71593650 Msa0182760:intron 25.0%
! AACAAAGAAAAACCACTATA+CGG - chr1_4:71593775-71593794 None:intergenic 25.0%
! AAGATTTATATAAGGCTTCT+CGG - chr1_4:71593959-71593978 None:intergenic 25.0%
AAGAAAGAAAGAGAGTGAAT+AGG - chr1_4:71591827-71591846 None:intergenic 30.0%
CTTTGATGAAACGTCAAAAA+AGG - chr1_4:71591926-71591945 None:intergenic 30.0%
AAAACAAACGATGGTTGTAA+TGG + chr1_4:71591990-71592009 Msa0182760:exon 30.0%
CAATGAGTCTATCTAATCAA+AGG + chr1_4:71592387-71592406 Msa0182760:intron 30.0%
TGTCATCATGACAAATATCA+AGG - chr1_4:71592423-71592442 None:intergenic 30.0%
TGATATTTGTCATGATGACA+TGG + chr1_4:71592423-71592442 Msa0182760:intron 30.0%
! TTTAGGATACTTCATTATGC+TGG + chr1_4:71592641-71592660 Msa0182760:intron 30.0%
AGTTGAAAATCTTGAAAGCA+AGG - chr1_4:71592758-71592777 None:intergenic 30.0%
!! TTTTGAGAATCCCTTTGTTT+TGG + chr1_4:71593052-71593071 Msa0182760:intron 30.0%
TGGTATGAAAGTAAACAACA+TGG - chr1_4:71593113-71593132 None:intergenic 30.0%
GTTTACTTTCATACCAGATT+TGG + chr1_4:71593117-71593136 Msa0182760:intron 30.0%
! GAGTTTAAAGTAACCAAATC+TGG - chr1_4:71593133-71593152 None:intergenic 30.0%
TTAAAGTAACCACATTTGAC+AGG - chr1_4:71593191-71593210 None:intergenic 30.0%
CATTGAGCTTTACAAAAAGT+TGG - chr1_4:71593384-71593403 None:intergenic 30.0%
TTTGTAAAGCTCAATGAAAC+AGG + chr1_4:71593388-71593407 Msa0182760:CDS 30.0%
GACAAAAATGACAGTTTACT+TGG - chr1_4:71593471-71593490 None:intergenic 30.0%
TACACATTTGAGGATCAATA+TGG + chr1_4:71593641-71593660 Msa0182760:intron 30.0%
GATGCATTTACATACTATAG+TGG + chr1_4:71593703-71593722 Msa0182760:intron 30.0%
TCTTTGTGTTGTGAAACAAA+TGG + chr1_4:71593975-71593994 Msa0182760:intron 30.0%
GATTTGAGTATATAGCAAGT+TGG + chr1_4:71594020-71594039 Msa0182760:intron 30.0%
TTCTATGTAAGATGAAGGAT+TGG - chr1_4:71594079-71594098 None:intergenic 30.0%
CCAATGAAATATTTCGAGAA+AGG - chr1_4:71594206-71594225 None:intergenic 30.0%
CCTTTCTCGAAATATTTCAT+TGG + chr1_4:71594203-71594222 Msa0182760:CDS 30.0%
!!! TGCTTCATTTTTTGTTGTAG+TGG + chr1_4:71594279-71594298 Msa0182760:three_prime_UTR 30.0%
TTAATTAGTCAGTTTCTCAG+CGG + chr1_4:71594315-71594334 Msa0182760:three_prime_UTR 30.0%
TTTCAGTATTGCTCTTTCAA+GGG - chr1_4:71594474-71594493 None:intergenic 30.0%
ATTTCAGTATTGCTCTTTCA+AGG - chr1_4:71594475-71594494 None:intergenic 30.0%
ATAAAGAAAATGTCAACCGC+TGG - chr1_4:71591743-71591762 None:intergenic 35.0%
AGAAAGAGAGTGAATAGGAA+GGG - chr1_4:71591822-71591841 None:intergenic 35.0%
AAGAAAGAGAGTGAATAGGA+AGG - chr1_4:71591823-71591842 None:intergenic 35.0%
!! TCTGTTCTTTTTCGTTGTCT+TGG + chr1_4:71592159-71592178 Msa0182760:CDS 35.0%
TGCTTTCAAATTCGCTGAAA+TGG + chr1_4:71592185-71592204 Msa0182760:CDS 35.0%
ATGACGATGATGATATACCT+TGG - chr1_4:71592220-71592239 None:intergenic 35.0%
! TCAAAGTGTCATTGCTTCAT+AGG + chr1_4:71592591-71592610 Msa0182760:intron 35.0%
ACTTTATACCCACGTATTTC+TGG + chr1_4:71592696-71592715 Msa0182760:intron 35.0%
TAGATAGACCAGAAATACGT+GGG - chr1_4:71592707-71592726 None:intergenic 35.0%
AGATGTAATTGACCATGATG+TGG + chr1_4:71592889-71592908 Msa0182760:CDS 35.0%
! TCGAACAACTTTTGTCAACT+AGG + chr1_4:71592996-71593015 Msa0182760:CDS 35.0%
TGACAGGAAAGTAAACAACA+TGG - chr1_4:71593175-71593194 None:intergenic 35.0%
GTTTACTTTCCTGTCAAATG+TGG + chr1_4:71593179-71593198 Msa0182760:intron 35.0%
! ACAAAAAGTTGGTCTCTTGT+TGG - chr1_4:71593373-71593392 None:intergenic 35.0%
TTTGTCTCTAAATGTGTGTG+AGG + chr1_4:71593485-71593504 Msa0182760:intron 35.0%
AGTAACATAAGACACACTTG+AGG - chr1_4:71593555-71593574 None:intergenic 35.0%
GTGTCTTATGTTTGTCAGTT+TGG - chr1_4:71593589-71593608 None:intergenic 35.0%
GAAAAACAGATTCCGTATAG+TGG + chr1_4:71593760-71593779 Msa0182760:intron 35.0%
AGGATGACATCTTACAAGAA+GGG - chr1_4:71593886-71593905 None:intergenic 35.0%
TAGGATGACATCTTACAAGA+AGG - chr1_4:71593887-71593906 None:intergenic 35.0%
CTCGAAATATTTCATTGGAC+TGG + chr1_4:71594208-71594227 Msa0182760:CDS 35.0%
TATGAATACGGGTGTGTATG+AGG - chr1_4:71591786-71591805 None:intergenic 40.0%
TCATACACACCCGTATTCAT+AGG + chr1_4:71591785-71591804 Msa0182760:five_prime_UTR 40.0%
CATACACACCCGTATTCATA+GGG + chr1_4:71591786-71591805 Msa0182760:five_prime_UTR 40.0%
AGGAAGGTTCCCTATGAATA+CGG - chr1_4:71591798-71591817 None:intergenic 40.0%
GGAGTCTTACGATGAAATGT+CGG - chr1_4:71591905-71591924 None:intergenic 40.0%
AGGATACGCAATCGCTAAAT+CGG - chr1_4:71592022-71592041 None:intergenic 40.0%
CTTTGTGGAAAGATCCTCAA+AGG - chr1_4:71592064-71592083 None:intergenic 40.0%
! CTTTGAGGATCTTTCCACAA+AGG + chr1_4:71592062-71592081 Msa0182760:CDS 40.0%
GATCTTTCCACAAAGGTTCA+GGG + chr1_4:71592069-71592088 Msa0182760:CDS 40.0%
AAGGTTCAGGGAATCAAGAA+CGG + chr1_4:71592081-71592100 Msa0182760:CDS 40.0%
TAAGCGAATATGTTGAGTGG+AGG - chr1_4:71592109-71592128 None:intergenic 40.0%
GTCTAAGCGAATATGTTGAG+TGG - chr1_4:71592112-71592131 None:intergenic 40.0%
TGAAATGGAATACGTTGCCA+AGG + chr1_4:71592200-71592219 Msa0182760:CDS 40.0%
ACACCTTCAATGTGAAGTGT+CGG - chr1_4:71592486-71592505 None:intergenic 40.0%
! AGTGTCATTGCTTCATAGGT+AGG + chr1_4:71592595-71592614 Msa0182760:intron 40.0%
CTAGATAGACCAGAAATACG+TGG - chr1_4:71592708-71592727 None:intergenic 40.0%
! AGATGTTTTCACTGTGTCAC+GGG - chr1_4:71592915-71592934 None:intergenic 40.0%
! CAGATGTTTTCACTGTGTCA+CGG - chr1_4:71592916-71592935 None:intergenic 40.0%
!!! TGAGAATCCCTTTGTTTTGG+AGG + chr1_4:71593055-71593074 Msa0182760:intron 40.0%
!!! GAGAATCCCTTTGTTTTGGA+GGG + chr1_4:71593056-71593075 Msa0182760:intron 40.0%
!!! AGAATCCCTTTGTTTTGGAG+GGG + chr1_4:71593057-71593076 Msa0182760:intron 40.0%
AACCTCAATCCTAACATGTG+AGG + chr1_4:71593848-71593867 Msa0182760:intron 40.0%
TCTTACAAGAAGGGTCATCT+AGG - chr1_4:71593877-71593896 None:intergenic 40.0%
GAAAGGGTTATTCGCATGTT+AGG - chr1_4:71593906-71593925 None:intergenic 40.0%
TGTTATTGCAGAGCAATCAG+CGG + chr1_4:71594116-71594135 Msa0182760:intron 40.0%
GTATTCTATAACTGGACGTG+AGG - chr1_4:71594169-71594188 None:intergenic 40.0%
GCATTGTACTGCTATACCAA+TGG - chr1_4:71594262-71594281 None:intergenic 40.0%
AGCGGCTATTTGGAGATTTA+GGG + chr1_4:71594333-71594352 Msa0182760:three_prime_UTR 40.0%
GGAAGGTTCCCTATGAATAC+GGG - chr1_4:71591797-71591816 None:intergenic 45.0%
AGTGAATAGGAAGGGAAGGA+AGG - chr1_4:71591814-71591833 None:intergenic 45.0%
AGAGAGTGAATAGGAAGGGA+AGG - chr1_4:71591818-71591837 None:intergenic 45.0%
GGAAAGATCCTCAAAGGCTT+CGG - chr1_4:71592058-71592077 None:intergenic 45.0%
GGATCTTTCCACAAAGGTTC+AGG + chr1_4:71592068-71592087 Msa0182760:CDS 45.0%
CTTGATTCCCTGAACCTTTG+TGG - chr1_4:71592079-71592098 None:intergenic 45.0%
! GTACCGACACTTCACATTGA+AGG + chr1_4:71592480-71592499 Msa0182760:intron 45.0%
! CTTCACATTGAAGGTGTGTC+CGG + chr1_4:71592489-71592508 Msa0182760:intron 45.0%
!! GCTGGTTGTTTTCTCGCTTT+TGG + chr1_4:71592659-71592678 Msa0182760:intron 45.0%
GATTGGTATGCTCAGCTACA+TGG + chr1_4:71592830-71592849 Msa0182760:intron 45.0%
CGTGACACAGTGAAAACATC+TGG + chr1_4:71592914-71592933 Msa0182760:CDS 45.0%
TTTGTGCACCGTATCACTCA+TGG + chr1_4:71593308-71593327 Msa0182760:CDS 45.0%
TTGTGCACCGTATCACTCAT+GGG + chr1_4:71593309-71593328 Msa0182760:CDS 45.0%
! TCCACGAATGTGTCTTTTGC+TGG - chr1_4:71593667-71593686 None:intergenic 45.0%
TGCCTCACATGTTAGGATTG+AGG - chr1_4:71593853-71593872 None:intergenic 45.0%
TCTAGGTTGCCTCACATGTT+AGG - chr1_4:71593860-71593879 None:intergenic 45.0%
ATAAGGCTTCTCGGCTAAGT+TGG - chr1_4:71593950-71593969 None:intergenic 45.0%
TGTTGTAGTGGCGATCTGTT+CGG + chr1_4:71594291-71594310 Msa0182760:three_prime_UTR 45.0%
TCAGTTTCTCAGCGGCTATT+TGG + chr1_4:71594323-71594342 Msa0182760:three_prime_UTR 45.0%
CAGCGGCTATTTGGAGATTT+AGG + chr1_4:71594332-71594351 Msa0182760:three_prime_UTR 45.0%
ATCCTCAAAGGCTTCGGCAA+TGG - chr1_4:71592052-71592071 None:intergenic 50.0%
GTATGCTCAGCTACATGGAC+TGG + chr1_4:71592835-71592854 Msa0182760:intron 50.0%
TAATCTACGCAGAGTTCGCC+AGG + chr1_4:71592949-71592968 Msa0182760:CDS 50.0%
AATCTACGCAGAGTTCGCCA+GGG + chr1_4:71592950-71592969 Msa0182760:CDS 50.0%
CTCTAACGAGATCAAGACCC+TGG - chr1_4:71592970-71592989 None:intergenic 50.0%
CCACACCCCTCCAAAACAAA+GGG - chr1_4:71593065-71593084 None:intergenic 50.0%
!!! CCTTTGTTTTGGAGGGGTGT+GGG + chr1_4:71593063-71593082 Msa0182760:intron 50.0%
GAAGTGGCGTCAACAGCTTA+TGG + chr1_4:71593280-71593299 Msa0182760:CDS 50.0%
TGTGCACCGTATCACTCATG+GGG + chr1_4:71593310-71593329 Msa0182760:CDS 50.0%
CTGACTCCCCATGAGTGATA+CGG - chr1_4:71593319-71593338 None:intergenic 50.0%
TCACTCATGGGGAGTCAGAA+CGG + chr1_4:71593321-71593340 Msa0182760:CDS 50.0%
GCCAGCAAAAGACACATTCG+TGG + chr1_4:71593663-71593682 Msa0182760:intron 50.0%
CAGCGGAGAAGAAGATGAGA+AGG + chr1_4:71594133-71594152 Msa0182760:CDS 50.0%
GCTTCGGCAATGGCAGAAAG+AGG - chr1_4:71592042-71592061 None:intergenic 55.0%
TGCCATTGCCGAAGCCTTTG+AGG + chr1_4:71592047-71592066 Msa0182760:CDS 55.0%
! ACATTGAAGGTGTGTCCGGC+TGG + chr1_4:71592493-71592512 Msa0182760:intron 55.0%
GTGTCTTGTCAGACACCAGC+CGG - chr1_4:71592511-71592530 None:intergenic 55.0%
!! TGCTCAGCTACATGGACTGG+TGG + chr1_4:71592838-71592857 Msa0182760:intron 55.0%
!!! CCCTTTGTTTTGGAGGGGTG+TGG + chr1_4:71593062-71593081 Msa0182760:intron 55.0%
CCCACACCCCTCCAAAACAA+AGG - chr1_4:71593066-71593085 None:intergenic 55.0%
GGCGTCAACAGCTTATGGAG+AGG + chr1_4:71593285-71593304 Msa0182760:CDS 55.0%
GTCAGAACGGCTGTGTATGC+TGG + chr1_4:71593334-71593353 Msa0182760:CDS 55.0%
! GGACTGGTGAGTTCTAGCAG+AGG + chr1_4:71594224-71594243 Msa0182760:exon 55.0%
GAGGCAGTGATGTGCTCCAT+TGG + chr1_4:71594243-71594262 Msa0182760:three_prime_UTR 55.0%
CTGTGTCACGGGCCACATCA+TGG - chr1_4:71592904-71592923 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 71591722 71594498 71591722 ID=Msa0182760;Name=Msa0182760
chr1_4 mRNA 71591722 71594498 71591722 ID=Msa0182760-mRNA-1;Parent=Msa0182760;Name=Msa0182760-mRNA-1;_AED=0.01;_eAED=0.01;_QI=278|1|1|1|1|1|4|265|215
chr1_4 exon 71591722 71592221 71591722 ID=Msa0182760-mRNA-1:exon:23969;Parent=Msa0182760-mRNA-1
chr1_4 exon 71592845 71593017 71592845 ID=Msa0182760-mRNA-1:exon:23970;Parent=Msa0182760-mRNA-1
chr1_4 exon 71593264 71593409 71593264 ID=Msa0182760-mRNA-1:exon:23971;Parent=Msa0182760-mRNA-1
chr1_4 exon 71594127 71594498 71594127 ID=Msa0182760-mRNA-1:exon:23972;Parent=Msa0182760-mRNA-1
chr1_4 five_prime_UTR 71591722 71591999 71591722 ID=Msa0182760-mRNA-1:five_prime_utr;Parent=Msa0182760-mRNA-1
chr1_4 CDS 71592000 71592221 71592000 ID=Msa0182760-mRNA-1:cds;Parent=Msa0182760-mRNA-1
chr1_4 CDS 71592845 71593017 71592845 ID=Msa0182760-mRNA-1:cds;Parent=Msa0182760-mRNA-1
chr1_4 CDS 71593264 71593409 71593264 ID=Msa0182760-mRNA-1:cds;Parent=Msa0182760-mRNA-1
chr1_4 CDS 71594127 71594233 71594127 ID=Msa0182760-mRNA-1:cds;Parent=Msa0182760-mRNA-1
chr1_4 three_prime_UTR 71594234 71594498 71594234 ID=Msa0182760-mRNA-1:three_prime_utr;Parent=Msa0182760-mRNA-1
Gene Sequence

>Msa0182760

ATGGTTGTAATGGATCATTCCGATTTAGCGATTGCGTATCCTCTTTCTGCCATTGCCGAAGCCTTTGAGGATCTTTCCACAAAGGTTCAGGGAATCAAGAACGGTTCCTCCACTCAACATATTCGCTTAGACTCTTTCTGTGAAATTGCTTCTCTTGTTTCTGTTCTTTTTCGTTGTCTTGGTCTTGCTTTCAAATTCGCTGAAATGGAATACGTTGCCAAGCTACATGGACTGGTGGAAGCATCAAAGAAGTATCAGACTCTACAAGATGTAATTGACCATGATGTGGCCCGTGACACAGTGAAAACATCTGGAAGCTATTCACGTAATCTACGCAGAGTTCGCCAGGGTCTTGATCTCGTTAGAGCTATATTCGAACAACTTTTGTCAACTAGTGATATTTATCTGAAAGAAGTGGCGTCAACAGCTTATGGAGAGGTTTGTGCACCGTATCACTCATGGGGAGTCAGAACGGCTGTGTATGCTGGAATGTATACACTTCCAACAAGAGACCAACTTTTTGTAAAGCTCAATGAAACAGAGCAATCAGCGGAGAAGAAGATGAGAAGGTACATTACTGCCTCACGTCCAGTTATAGAATACATTGATAAACTGTACCTTTCTCGAAATATTTCATTGGACTGGTGA
Protein sequence

>Msa0182760

MVVMDHSDLAIAYPLSAIAEAFEDLSTKVQGIKNGSSTQHIRLDSFCEIASLVSVLFRCLGLAFKFAEMEYVAKLHGLVEASKKYQTLQDVIDHDVARDTVKTSGSYSRNLRRVRQGLDLVRAIFEQLLSTSDIYLKEVASTAYGEVCAPYHSWGVRTAVYAGMYTLPTRDQLFVKLNETEQSAEKKMRRYITASRPVIEYIDKLYLSRNISLDW*