AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0184950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184950 MtrunA17_Chr3g0078271 96.875 160 4 1 1 159 1 160 4.37e-99 300
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 86 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCTTCTCTCCATTTCT+AGG 0.134175 1_4:-74553040 Msa0184950:five_prime_UTR
GGATATCAAAACGGAATTTC+TGG 0.258959 1_4:-74551297 Msa0184950:CDS
TAGCGGCGGTCACGATAGTT+AGG 0.305964 1_4:+74552755 None:intergenic
AAGAAGATGAAGAAATGAAA+TGG 0.316200 1_4:+74553060 None:intergenic
GGGTTGGGGTTAGGTGGAGG+TGG 0.324888 1_4:+74552914 None:intergenic
GGAGGTGGAGCGGTGGTGGT+TGG 0.356369 1_4:+74552953 None:intergenic
AAATCGCGACGACGAGGGTT+GGG 0.359076 1_4:+74552899 None:intergenic
CGCGGCCACCTCGGCCGCCA+CGG 0.365642 1_4:+74552837 None:intergenic
ATGAAGAAATGAAATGGGTT+TGG 0.371123 1_4:+74553066 None:intergenic
CGACGACGAGGGTTGGGGTT+AGG 0.385071 1_4:+74552905 None:intergenic
GGCCGAGGTGGCCGCGGTGG+AGG 0.393766 1_4:-74552830 Msa0184950:CDS
CGTGGCGGCCGAGGTGGCCG+CGG 0.394289 1_4:-74552836 Msa0184950:CDS
CCCCTTCTAGATCTCTTATA+CGG 0.400477 1_4:+74552695 None:intergenic
TATGAACGGGGAGGTGGAAG+GGG 0.400499 1_4:-74551372 Msa0184950:CDS
TTCTCTTCTCTCCATTTCTA+GGG 0.409479 1_4:-74553039 Msa0184950:five_prime_UTR
AGAAGATGAAGAAATGAAAT+GGG 0.414047 1_4:+74553061 None:intergenic
CGACGAGACTGCGGCGGAGG+AGG 0.415404 1_4:+74552728 None:intergenic
CGAGGGTTGGGGTTAGGTGG+AGG 0.418872 1_4:+74552911 None:intergenic
ATGAATGGAAACCCTAGAAA+TGG 0.419272 1_4:+74553028 None:intergenic
AGGAGGAGGTGGAGCGGTGG+TGG 0.419667 1_4:+74552949 None:intergenic
CGTCCTCACCGTGGCGGCCG+AGG 0.422279 1_4:-74552845 Msa0184950:CDS
CGAGGTGGCCGCGGTGGAGG+TGG 0.424378 1_4:-74552827 Msa0184950:CDS
GAACGGGGAGGTGGAAGGGG+TGG 0.427644 1_4:-74551369 Msa0184950:CDS
CACCTCCACCGCGGCCACCT+CGG 0.431991 1_4:+74552828 None:intergenic
TCACTTCGGTGGCTATGAAC+GGG 0.433155 1_4:-74551385 Msa0184950:CDS
GTTTCCATTCATCTCTTCAA+TGG 0.441656 1_4:-74553017 Msa0184950:exon
TTATGCAATTACCGGTCGTT+AGG 0.445933 1_4:+74552659 None:intergenic
GAACGACGAGACTGCGGCGG+AGG 0.450604 1_4:+74552725 None:intergenic
GGAGGTGGGAGATCGTCGTC+AGG 0.475713 1_4:+74552929 None:intergenic
CGCCGTATAAGAGATCTAGA+AGG 0.476081 1_4:-74552697 Msa0184950:CDS
GGTGGCTATGAACGGGGAGG+TGG 0.481462 1_4:-74551378 Msa0184950:CDS
GGCAGATTTCCACATCGATC+TGG 0.487532 1_4:-74551324 Msa0184950:CDS
GGCGGCCGAGGTGGCCGCGG+TGG 0.490769 1_4:-74552833 Msa0184950:CDS
GCTATGAACGGGGAGGTGGA+AGG 0.493281 1_4:-74551374 Msa0184950:CDS
AAAATCGCGACGACGAGGGT+TGG 0.499103 1_4:+74552898 None:intergenic
GGTTGGGGTTAGGTGGAGGT+GGG 0.500708 1_4:+74552915 None:intergenic
CTCGGCCGCCACGGTGAGGA+CGG 0.500922 1_4:+74552846 None:intergenic
CATAGCCACCGAAGTGATCA+TGG 0.502264 1_4:+74551391 None:intergenic
GAGCGGTGGTGGTTGGAGAT+CGG 0.506162 1_4:+74552960 None:intergenic
AATCGCGACGACGAGGGTTG+GGG 0.517751 1_4:+74552900 None:intergenic
CTATGAACGGGGAGGTGGAA+GGG 0.518205 1_4:-74551373 Msa0184950:CDS
AGATCGTCGTCAGGAGGAGG+TGG 0.519392 1_4:+74552938 None:intergenic
GGGAGATCGTCGTCAGGAGG+AGG 0.522110 1_4:+74552935 None:intergenic
ATCACTTCGGTGGCTATGAA+CGG 0.525110 1_4:-74551386 Msa0184950:CDS
CGGTCACGATAGTTAGGGCT+AGG 0.549799 1_4:+74552761 None:intergenic
TCGGCCATTGAAGAGATGAA+TGG 0.551754 1_4:+74553013 None:intergenic
CTAGATCTCTTATACGGCGG+AGG 0.558280 1_4:+74552701 None:intergenic
CCTCACCGTGGCGGCCGAGG+TGG 0.560575 1_4:-74552842 Msa0184950:CDS
CGATGTGGAAATCTGCCACC+AGG 0.571313 1_4:+74551330 None:intergenic
CTTCTAGATCTCTTATACGG+CGG 0.571503 1_4:+74552698 None:intergenic
GCCGTATAAGAGATCTAGAA+GGG 0.575206 1_4:-74552696 Msa0184950:CDS
TACCGGTCGTTAGGTCTATG+AGG 0.575928 1_4:+74552668 None:intergenic
AGCGGCGGTCACGATAGTTA+GGG 0.586078 1_4:+74552756 None:intergenic
GTCAGGAGGAGGTGGAGCGG+TGG 0.588117 1_4:+74552946 None:intergenic
CCACCTCGGCCGCCACGGTG+AGG 0.591302 1_4:+74552842 None:intergenic
TTTCCACATCGATCTGGAGG+GGG 0.594827 1_4:-74551318 Msa0184950:CDS
GGAGAACGACGAGACTGCGG+CGG 0.603253 1_4:+74552722 None:intergenic
AGATTTCCACATCGATCTGG+AGG 0.603786 1_4:-74551321 Msa0184950:CDS
GGTGGGAGATCGTCGTCAGG+AGG 0.604290 1_4:+74552932 None:intergenic
CGACGAGGGTTGGGGTTAGG+TGG 0.605859 1_4:+74552908 None:intergenic
GGCCGCCACGGTGAGGACGG+CGG 0.613259 1_4:+74552849 None:intergenic
GGCGTTTGATGTCTCTATCA+CGG 0.614422 1_4:+74552792 None:intergenic
ATTTCCACATCGATCTGGAG+GGG 0.621278 1_4:-74551319 Msa0184950:CDS
GTCGTCAGGAGGAGGTGGAG+CGG 0.622070 1_4:+74552943 None:intergenic
TTTGGCCATGATCACTTCGG+TGG 0.625230 1_4:-74551396 Msa0184950:CDS
GGAGGAGAACGACGAGACTG+CGG 0.628983 1_4:+74552719 None:intergenic
GTCCTCATAGACCTAACGAC+CGG 0.629453 1_4:-74552670 Msa0184950:intron
GATTTCCACATCGATCTGGA+GGG 0.634289 1_4:-74551320 Msa0184950:CDS
CACTTCGGTGGCTATGAACG+GGG 0.634430 1_4:-74551384 Msa0184950:CDS
AGGTTTGGCCATGATCACTT+CGG 0.635013 1_4:-74551399 Msa0184950:CDS
GACCGCCGTCCTCACCGTGG+CGG 0.636148 1_4:-74552851 Msa0184950:CDS
GGTCGTGAGATTCGAGAGGG+AGG 0.640564 1_4:+74552981 None:intergenic
CGGTCAAAATCGCGACGACG+AGG 0.651901 1_4:+74552893 None:intergenic
GGCTATGCAGATGATAGACC+TGG 0.653573 1_4:-74551348 Msa0184950:CDS
GCGGCGGAGGAGGAGAATAG+CGG 0.655828 1_4:+74552738 None:intergenic
GGTCAAAATCGCGACGACGA+GGG 0.656572 1_4:+74552894 None:intergenic
ATCGGTCGTGAGATTCGAGA+GGG 0.664847 1_4:+74552978 None:intergenic
GCGGAGGAGGAGAATAGCGG+CGG 0.691138 1_4:+74552741 None:intergenic
TTCGGTGGCTATGAACGGGG+AGG 0.691284 1_4:-74551381 Msa0184950:CDS
CGAAATCTCTGTCGTCACGA+CGG 0.694181 1_4:+74552873 None:intergenic
GATCGGTCGTGAGATTCGAG+AGG 0.698207 1_4:+74552977 None:intergenic
TTCGACCGCCGTCCTCACCG+TGG 0.713062 1_4:-74552854 Msa0184950:CDS
CCGTATAAGAGATCTAGAAG+GGG 0.715875 1_4:-74552695 Msa0184950:CDS
TATGCAGATGATAGACCTGG+TGG 0.763982 1_4:-74551345 Msa0184950:CDS
TGTAGTAACCACCTCCACCG+CGG 0.784401 1_4:+74552819 None:intergenic
AGTTAGGGCTAGGACTACGA+CGG 0.787338 1_4:+74552771 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTGATAGCATTTAATAA+CGG - chr1_4:74551806-74551825 Msa0184950:intron 15.0%
!! ACTTATTTGAATTTATCTAT+TGG - chr1_4:74552186-74552205 Msa0184950:intron 15.0%
!!! ATTTCAAATAAGTTTTATCT+TGG + chr1_4:74552296-74552315 None:intergenic 15.0%
!!! TAATTGATAGCATTTAATAA+CGG - chr1_4:74551806-74551825 Msa0184950:intron 15.0%
!! ACTTATTTGAATTTATCTAT+TGG - chr1_4:74552186-74552205 Msa0184950:intron 15.0%
!!! ATTTCAAATAAGTTTTATCT+TGG + chr1_4:74552296-74552315 None:intergenic 15.0%
!! TTCAATTATTATGCAATTAC+CGG + chr1_4:74551746-74551765 None:intergenic 20.0%
!!! TAGTAATTGATAGCATTTAA+TGG - chr1_4:74551853-74551872 Msa0184950:intron 20.0%
!!! AGTAATTGATAGCATTTAAT+GGG - chr1_4:74551804-74551823 Msa0184950:intron 20.0%
!! ATTTATCTTAACTGTAGATT+AGG - chr1_4:74551886-74551905 Msa0184950:intron 20.0%
!! TTTATCTTAACTGTAGATTA+GGG - chr1_4:74551887-74551906 Msa0184950:intron 20.0%
!! AACCATTGTTTATAGTTATA+TGG - chr1_4:74552150-74552169 Msa0184950:intron 20.0%
!!! TGTTTGTTGTATAATGTAAA+AGG - chr1_4:74552672-74552691 Msa0184950:CDS 20.0%
!!! TTTAAAGTCATGTATTTGAT+TGG - chr1_4:74552794-74552813 Msa0184950:CDS 20.0%
!! TTCAATTATTATGCAATTAC+CGG + chr1_4:74551746-74551765 None:intergenic 20.0%
!!! TAGTAATTGATAGCATTTAA+TGG - chr1_4:74551853-74551872 Msa0184950:intron 20.0%
!!! AGTAATTGATAGCATTTAAT+GGG - chr1_4:74551804-74551823 Msa0184950:intron 20.0%
!! ATTTATCTTAACTGTAGATT+AGG - chr1_4:74551886-74551905 Msa0184950:intron 20.0%
!! TTTATCTTAACTGTAGATTA+GGG - chr1_4:74551887-74551906 Msa0184950:intron 20.0%
!! AACCATTGTTTATAGTTATA+TGG - chr1_4:74552150-74552169 Msa0184950:intron 20.0%
!!! TGTTTGTTGTATAATGTAAA+AGG - chr1_4:74552672-74552691 Msa0184950:CDS 20.0%
!!! TTTAAAGTCATGTATTTGAT+TGG - chr1_4:74552794-74552813 Msa0184950:CDS 20.0%
! AGAAGATGAAGAAATGAAAT+GGG + chr1_4:74551336-74551355 None:intergenic 25.0%
! AAGAAGATGAAGAAATGAAA+TGG + chr1_4:74551337-74551356 None:intergenic 25.0%
! CTTTAATCGTTTAGTAATTG+AGG - chr1_4:74551773-74551792 Msa0184950:intron 25.0%
! TAATAACGGTTTAGTAATTG+AGG - chr1_4:74551820-74551839 Msa0184950:intron 25.0%
!! TTTATCTTTTCCGATAGAAA+TGG - chr1_4:74552403-74552422 Msa0184950:intron 25.0%
! AACATGTTGAATATAACTTC+TGG + chr1_4:74552496-74552515 None:intergenic 25.0%
! ATAAAGTAGTCAGTTACAAT+TGG + chr1_4:74552576-74552595 None:intergenic 25.0%
! ACTGACTACTTTATAATGAA+AGG - chr1_4:74552582-74552601 Msa0184950:intron 25.0%
!! TCATGTACTTAACTTTTCAT+GGG - chr1_4:74552626-74552645 Msa0184950:intron 25.0%
! GCAAAGAAAACAAATAATAC+AGG + chr1_4:74552901-74552920 None:intergenic 25.0%
! AGAAGATGAAGAAATGAAAT+GGG + chr1_4:74551336-74551355 None:intergenic 25.0%
! AAGAAGATGAAGAAATGAAA+TGG + chr1_4:74551337-74551356 None:intergenic 25.0%
! CTTTAATCGTTTAGTAATTG+AGG - chr1_4:74551773-74551792 Msa0184950:intron 25.0%
! TAATAACGGTTTAGTAATTG+AGG - chr1_4:74551820-74551839 Msa0184950:intron 25.0%
!! TTTATCTTTTCCGATAGAAA+TGG - chr1_4:74552403-74552422 Msa0184950:intron 25.0%
! AACATGTTGAATATAACTTC+TGG + chr1_4:74552496-74552515 None:intergenic 25.0%
! ATAAAGTAGTCAGTTACAAT+TGG + chr1_4:74552576-74552595 None:intergenic 25.0%
! ACTGACTACTTTATAATGAA+AGG - chr1_4:74552582-74552601 Msa0184950:intron 25.0%
!! TCATGTACTTAACTTTTCAT+GGG - chr1_4:74552626-74552645 Msa0184950:intron 25.0%
! GCAAAGAAAACAAATAATAC+AGG + chr1_4:74552901-74552920 None:intergenic 25.0%
!!! GTTTTTTGATTTGAGGGTTT+GGG + chr1_4:74551309-74551328 None:intergenic 30.0%
!!! GGTTTGGTTTTTTGATTTGA+GGG + chr1_4:74551315-74551334 None:intergenic 30.0%
! ATGAAGAAATGAAATGGGTT+TGG + chr1_4:74551331-74551350 None:intergenic 30.0%
!! TTGATAGCATTTAATGGGTT+AGG - chr1_4:74551859-74551878 Msa0184950:intron 30.0%
! GTGCAAGAAATTTTAGATTG+CGG - chr1_4:74551934-74551953 Msa0184950:intron 30.0%
GGCCATATAACTATAAACAA+TGG + chr1_4:74552155-74552174 None:intergenic 30.0%
TTATAGTTATATGGCCTGTT+TGG - chr1_4:74552159-74552178 Msa0184950:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG + chr1_4:74552176-74552195 None:intergenic 30.0%
! TAAGTTTCGTGGTAATGTTT+GGG - chr1_4:74552211-74552230 Msa0184950:intron 30.0%
!! GTTGTTTTCATATGCTATCT+TGG + chr1_4:74552351-74552370 None:intergenic 30.0%
TAAATGAAATTGTGTGCCTA+TGG - chr1_4:74552516-74552535 Msa0184950:intron 30.0%
AGAAACCATAAAATGTCCAT+AGG + chr1_4:74552535-74552554 None:intergenic 30.0%
! GTCATGTACTTAACTTTTCA+TGG - chr1_4:74552625-74552644 Msa0184950:intron 30.0%
!! TTTTCATGGGATTTGTTTGA+AGG - chr1_4:74552639-74552658 Msa0184950:intron 30.0%
! TCATGTATTTGATTGGTTGT+TGG - chr1_4:74552801-74552820 Msa0184950:CDS 30.0%
AACATAGTTTCTGATGCATA+GGG + chr1_4:74552933-74552952 None:intergenic 30.0%
TAACATAGTTTCTGATGCAT+AGG + chr1_4:74552934-74552953 None:intergenic 30.0%
!!! GTTTTTTGATTTGAGGGTTT+GGG + chr1_4:74551309-74551328 None:intergenic 30.0%
!!! GGTTTGGTTTTTTGATTTGA+GGG + chr1_4:74551315-74551334 None:intergenic 30.0%
! ATGAAGAAATGAAATGGGTT+TGG + chr1_4:74551331-74551350 None:intergenic 30.0%
!! TTGATAGCATTTAATGGGTT+AGG - chr1_4:74551859-74551878 Msa0184950:intron 30.0%
! GTGCAAGAAATTTTAGATTG+CGG - chr1_4:74551934-74551953 Msa0184950:intron 30.0%
GGCCATATAACTATAAACAA+TGG + chr1_4:74552155-74552174 None:intergenic 30.0%
TTATAGTTATATGGCCTGTT+TGG - chr1_4:74552159-74552178 Msa0184950:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG + chr1_4:74552176-74552195 None:intergenic 30.0%
! TAAGTTTCGTGGTAATGTTT+GGG - chr1_4:74552211-74552230 Msa0184950:intron 30.0%
!! GTTGTTTTCATATGCTATCT+TGG + chr1_4:74552351-74552370 None:intergenic 30.0%
TAAATGAAATTGTGTGCCTA+TGG - chr1_4:74552516-74552535 Msa0184950:intron 30.0%
AGAAACCATAAAATGTCCAT+AGG + chr1_4:74552535-74552554 None:intergenic 30.0%
! GTCATGTACTTAACTTTTCA+TGG - chr1_4:74552625-74552644 Msa0184950:intron 30.0%
!! TTTTCATGGGATTTGTTTGA+AGG - chr1_4:74552639-74552658 Msa0184950:intron 30.0%
! TCATGTATTTGATTGGTTGT+TGG - chr1_4:74552801-74552820 Msa0184950:CDS 30.0%
AACATAGTTTCTGATGCATA+GGG + chr1_4:74552933-74552952 None:intergenic 30.0%
TAACATAGTTTCTGATGCAT+AGG + chr1_4:74552934-74552953 None:intergenic 30.0%
!!! GGTTTTTTGATTTGAGGGTT+TGG + chr1_4:74551310-74551329 None:intergenic 35.0%
!!! GGGTTTGGTTTTTTGATTTG+AGG + chr1_4:74551316-74551335 None:intergenic 35.0%
TTCTCTTCTCTCCATTTCTA+GGG - chr1_4:74551355-74551374 Msa0184950:CDS 35.0%
ATGAATGGAAACCCTAGAAA+TGG + chr1_4:74551369-74551388 None:intergenic 35.0%
GTTTCCATTCATCTCTTCAA+TGG - chr1_4:74551377-74551396 Msa0184950:CDS 35.0%
ATCTATTGGAGTAAGTTTCG+TGG - chr1_4:74552200-74552219 Msa0184950:intron 35.0%
! GTAAGTTTCGTGGTAATGTT+TGG - chr1_4:74552210-74552229 Msa0184950:intron 35.0%
CTTGCATAAGCCATTTCTAT+CGG + chr1_4:74552416-74552435 None:intergenic 35.0%
! TATAACTTCTGGCACTGTTT+TGG + chr1_4:74552485-74552504 None:intergenic 35.0%
CACTGTTACTCAATCTTATC+TGG - chr1_4:74552733-74552752 Msa0184950:CDS 35.0%
TGTTACTCAATCTTATCTGG+CGG - chr1_4:74552736-74552755 Msa0184950:CDS 35.0%
! TGGCGGATCATTAAAAACAT+TGG - chr1_4:74552753-74552772 Msa0184950:CDS 35.0%
TTTCTGATGCATAGGGTAAA+TGG + chr1_4:74552926-74552945 None:intergenic 35.0%
ATAACAAAGCAATTGGAGGA+GGG + chr1_4:74552964-74552983 None:intergenic 35.0%
TACGCACATAACAAAGCAAT+TGG + chr1_4:74552971-74552990 None:intergenic 35.0%
! CTTTGTTATGTGCGTAAAAC+AGG - chr1_4:74552975-74552994 Msa0184950:CDS 35.0%
TTATGTGCGTAAAACAGGTT+TGG - chr1_4:74552980-74552999 Msa0184950:CDS 35.0%
GGATATCAAAACGGAATTTC+TGG - chr1_4:74553097-74553116 Msa0184950:five_prime_UTR 35.0%
!!! GGTTTTTTGATTTGAGGGTT+TGG + chr1_4:74551310-74551329 None:intergenic 35.0%
!!! GGGTTTGGTTTTTTGATTTG+AGG + chr1_4:74551316-74551335 None:intergenic 35.0%
TTCTCTTCTCTCCATTTCTA+GGG - chr1_4:74551355-74551374 Msa0184950:CDS 35.0%
ATGAATGGAAACCCTAGAAA+TGG + chr1_4:74551369-74551388 None:intergenic 35.0%
GTTTCCATTCATCTCTTCAA+TGG - chr1_4:74551377-74551396 Msa0184950:CDS 35.0%
ATCTATTGGAGTAAGTTTCG+TGG - chr1_4:74552200-74552219 Msa0184950:intron 35.0%
! GTAAGTTTCGTGGTAATGTT+TGG - chr1_4:74552210-74552229 Msa0184950:intron 35.0%
CTTGCATAAGCCATTTCTAT+CGG + chr1_4:74552416-74552435 None:intergenic 35.0%
! TATAACTTCTGGCACTGTTT+TGG + chr1_4:74552485-74552504 None:intergenic 35.0%
CACTGTTACTCAATCTTATC+TGG - chr1_4:74552733-74552752 Msa0184950:CDS 35.0%
TGTTACTCAATCTTATCTGG+CGG - chr1_4:74552736-74552755 Msa0184950:CDS 35.0%
! TGGCGGATCATTAAAAACAT+TGG - chr1_4:74552753-74552772 Msa0184950:CDS 35.0%
TTTCTGATGCATAGGGTAAA+TGG + chr1_4:74552926-74552945 None:intergenic 35.0%
ATAACAAAGCAATTGGAGGA+GGG + chr1_4:74552964-74552983 None:intergenic 35.0%
TACGCACATAACAAAGCAAT+TGG + chr1_4:74552971-74552990 None:intergenic 35.0%
! CTTTGTTATGTGCGTAAAAC+AGG - chr1_4:74552975-74552994 Msa0184950:CDS 35.0%
TTATGTGCGTAAAACAGGTT+TGG - chr1_4:74552980-74552999 Msa0184950:CDS 35.0%
GGATATCAAAACGGAATTTC+TGG - chr1_4:74553097-74553116 Msa0184950:five_prime_UTR 35.0%
CTTCTCTTCTCTCCATTTCT+AGG - chr1_4:74551354-74551373 Msa0184950:CDS 40.0%
CTTCTAGATCTCTTATACGG+CGG + chr1_4:74551699-74551718 None:intergenic 40.0%
GCCGTATAAGAGATCTAGAA+GGG - chr1_4:74551698-74551717 Msa0184950:intron 40.0%
CCCCTTCTAGATCTCTTATA+CGG + chr1_4:74551702-74551721 None:intergenic 40.0%
CCGTATAAGAGATCTAGAAG+GGG - chr1_4:74551699-74551718 Msa0184950:intron 40.0%
TTATGCAATTACCGGTCGTT+AGG + chr1_4:74551738-74551757 None:intergenic 40.0%
! GTGTGCCTATGGACATTTTA+TGG - chr1_4:74552527-74552546 Msa0184950:intron 40.0%
CATAACAAAGCAATTGGAGG+AGG + chr1_4:74552965-74552984 None:intergenic 40.0%
GCACATAACAAAGCAATTGG+AGG + chr1_4:74552968-74552987 None:intergenic 40.0%
CTTCTCTTCTCTCCATTTCT+AGG - chr1_4:74551354-74551373 Msa0184950:CDS 40.0%
CTTCTAGATCTCTTATACGG+CGG + chr1_4:74551699-74551718 None:intergenic 40.0%
GCCGTATAAGAGATCTAGAA+GGG - chr1_4:74551698-74551717 Msa0184950:intron 40.0%
CCCCTTCTAGATCTCTTATA+CGG + chr1_4:74551702-74551721 None:intergenic 40.0%
CCGTATAAGAGATCTAGAAG+GGG - chr1_4:74551699-74551718 Msa0184950:intron 40.0%
TTATGCAATTACCGGTCGTT+AGG + chr1_4:74551738-74551757 None:intergenic 40.0%
! GTGTGCCTATGGACATTTTA+TGG - chr1_4:74552527-74552546 Msa0184950:intron 40.0%
CATAACAAAGCAATTGGAGG+AGG + chr1_4:74552965-74552984 None:intergenic 40.0%
GCACATAACAAAGCAATTGG+AGG + chr1_4:74552968-74552987 None:intergenic 40.0%
TCGGCCATTGAAGAGATGAA+TGG + chr1_4:74551384-74551403 None:intergenic 45.0%
GGCGTTTGATGTCTCTATCA+CGG + chr1_4:74551605-74551624 None:intergenic 45.0%
CGCCGTATAAGAGATCTAGA+AGG - chr1_4:74551697-74551716 Msa0184950:intron 45.0%
AGGTTTGGCCATGATCACTT+CGG - chr1_4:74552995-74553014 Msa0184950:CDS 45.0%
ATCACTTCGGTGGCTATGAA+CGG - chr1_4:74553008-74553027 Msa0184950:exon 45.0%
TATGCAGATGATAGACCTGG+TGG - chr1_4:74553049-74553068 Msa0184950:five_prime_UTR 45.0%
AGATTTCCACATCGATCTGG+AGG - chr1_4:74553073-74553092 Msa0184950:five_prime_UTR 45.0%
GATTTCCACATCGATCTGGA+GGG - chr1_4:74553074-74553093 Msa0184950:five_prime_UTR 45.0%
ATTTCCACATCGATCTGGAG+GGG - chr1_4:74553075-74553094 Msa0184950:five_prime_UTR 45.0%
TCTGGAGGGGGATATCAAAA+CGG - chr1_4:74553088-74553107 Msa0184950:five_prime_UTR 45.0%
TCGGCCATTGAAGAGATGAA+TGG + chr1_4:74551384-74551403 None:intergenic 45.0%
GGCGTTTGATGTCTCTATCA+CGG + chr1_4:74551605-74551624 None:intergenic 45.0%
CGCCGTATAAGAGATCTAGA+AGG - chr1_4:74551697-74551716 Msa0184950:intron 45.0%
AGGTTTGGCCATGATCACTT+CGG - chr1_4:74552995-74553014 Msa0184950:CDS 45.0%
ATCACTTCGGTGGCTATGAA+CGG - chr1_4:74553008-74553027 Msa0184950:exon 45.0%
TATGCAGATGATAGACCTGG+TGG - chr1_4:74553049-74553068 Msa0184950:five_prime_UTR 45.0%
AGATTTCCACATCGATCTGG+AGG - chr1_4:74553073-74553092 Msa0184950:five_prime_UTR 45.0%
GATTTCCACATCGATCTGGA+GGG - chr1_4:74553074-74553093 Msa0184950:five_prime_UTR 45.0%
ATTTCCACATCGATCTGGAG+GGG - chr1_4:74553075-74553094 Msa0184950:five_prime_UTR 45.0%
TCTGGAGGGGGATATCAAAA+CGG - chr1_4:74553088-74553107 Msa0184950:five_prime_UTR 45.0%
! GAGAGGGAGGTTTTCGACTT+CGG + chr1_4:74551403-74551422 None:intergenic 50.0%
ATCGGTCGTGAGATTCGAGA+GGG + chr1_4:74551419-74551438 None:intergenic 50.0%
CGAAATCTCTGTCGTCACGA+CGG + chr1_4:74551524-74551543 None:intergenic 50.0%
AGTTAGGGCTAGGACTACGA+CGG + chr1_4:74551626-74551645 None:intergenic 50.0%
CTAGATCTCTTATACGGCGG+AGG + chr1_4:74551696-74551715 None:intergenic 50.0%
GTCCTCATAGACCTAACGAC+CGG - chr1_4:74551724-74551743 Msa0184950:intron 50.0%
TACCGGTCGTTAGGTCTATG+AGG + chr1_4:74551729-74551748 None:intergenic 50.0%
TTTGGCCATGATCACTTCGG+TGG - chr1_4:74552998-74553017 Msa0184950:CDS 50.0%
CATAGCCACCGAAGTGATCA+TGG + chr1_4:74553006-74553025 None:intergenic 50.0%
TCACTTCGGTGGCTATGAAC+GGG - chr1_4:74553009-74553028 Msa0184950:exon 50.0%
GGCTATGCAGATGATAGACC+TGG - chr1_4:74553046-74553065 Msa0184950:five_prime_UTR 50.0%
GGCAGATTTCCACATCGATC+TGG - chr1_4:74553070-74553089 Msa0184950:five_prime_UTR 50.0%
TTTCCACATCGATCTGGAGG+GGG - chr1_4:74553076-74553095 Msa0184950:five_prime_UTR 50.0%
! GAGAGGGAGGTTTTCGACTT+CGG + chr1_4:74551403-74551422 None:intergenic 50.0%
ATCGGTCGTGAGATTCGAGA+GGG + chr1_4:74551419-74551438 None:intergenic 50.0%
CGAAATCTCTGTCGTCACGA+CGG + chr1_4:74551524-74551543 None:intergenic 50.0%
AGTTAGGGCTAGGACTACGA+CGG + chr1_4:74551626-74551645 None:intergenic 50.0%
CTAGATCTCTTATACGGCGG+AGG + chr1_4:74551696-74551715 None:intergenic 50.0%
GTCCTCATAGACCTAACGAC+CGG - chr1_4:74551724-74551743 Msa0184950:intron 50.0%
TACCGGTCGTTAGGTCTATG+AGG + chr1_4:74551729-74551748 None:intergenic 50.0%
TTTGGCCATGATCACTTCGG+TGG - chr1_4:74552998-74553017 Msa0184950:CDS 50.0%
CATAGCCACCGAAGTGATCA+TGG + chr1_4:74553006-74553025 None:intergenic 50.0%
TCACTTCGGTGGCTATGAAC+GGG - chr1_4:74553009-74553028 Msa0184950:exon 50.0%
GGCTATGCAGATGATAGACC+TGG - chr1_4:74553046-74553065 Msa0184950:five_prime_UTR 50.0%
GGCAGATTTCCACATCGATC+TGG - chr1_4:74553070-74553089 Msa0184950:five_prime_UTR 50.0%
TTTCCACATCGATCTGGAGG+GGG - chr1_4:74553076-74553095 Msa0184950:five_prime_UTR 50.0%
GATCGGTCGTGAGATTCGAG+AGG + chr1_4:74551420-74551439 None:intergenic 55.0%
AAATCGCGACGACGAGGGTT+GGG + chr1_4:74551498-74551517 None:intergenic 55.0%
AAAATCGCGACGACGAGGGT+TGG + chr1_4:74551499-74551518 None:intergenic 55.0%
GGTCAAAATCGCGACGACGA+GGG + chr1_4:74551503-74551522 None:intergenic 55.0%
TGTAGTAACCACCTCCACCG+CGG + chr1_4:74551578-74551597 None:intergenic 55.0%
CGGTCACGATAGTTAGGGCT+AGG + chr1_4:74551636-74551655 None:intergenic 55.0%
AGCGGCGGTCACGATAGTTA+GGG + chr1_4:74551641-74551660 None:intergenic 55.0%
TAGCGGCGGTCACGATAGTT+AGG + chr1_4:74551642-74551661 None:intergenic 55.0%
CACTTCGGTGGCTATGAACG+GGG - chr1_4:74553010-74553029 Msa0184950:exon 55.0%
CTATGAACGGGGAGGTGGAA+GGG - chr1_4:74553021-74553040 Msa0184950:five_prime_UTR 55.0%
TATGAACGGGGAGGTGGAAG+GGG - chr1_4:74553022-74553041 Msa0184950:five_prime_UTR 55.0%
CGATGTGGAAATCTGCCACC+AGG + chr1_4:74553067-74553086 None:intergenic 55.0%
TATCCCCCTCCAGATCGATG+TGG + chr1_4:74553082-74553101 None:intergenic 55.0%
GATCGGTCGTGAGATTCGAG+AGG + chr1_4:74551420-74551439 None:intergenic 55.0%
AAATCGCGACGACGAGGGTT+GGG + chr1_4:74551498-74551517 None:intergenic 55.0%
AAAATCGCGACGACGAGGGT+TGG + chr1_4:74551499-74551518 None:intergenic 55.0%
GGTCAAAATCGCGACGACGA+GGG + chr1_4:74551503-74551522 None:intergenic 55.0%
TGTAGTAACCACCTCCACCG+CGG + chr1_4:74551578-74551597 None:intergenic 55.0%
CGGTCACGATAGTTAGGGCT+AGG + chr1_4:74551636-74551655 None:intergenic 55.0%
AGCGGCGGTCACGATAGTTA+GGG + chr1_4:74551641-74551660 None:intergenic 55.0%
TAGCGGCGGTCACGATAGTT+AGG + chr1_4:74551642-74551661 None:intergenic 55.0%
CACTTCGGTGGCTATGAACG+GGG - chr1_4:74553010-74553029 Msa0184950:exon 55.0%
CTATGAACGGGGAGGTGGAA+GGG - chr1_4:74553021-74553040 Msa0184950:five_prime_UTR 55.0%
TATGAACGGGGAGGTGGAAG+GGG - chr1_4:74553022-74553041 Msa0184950:five_prime_UTR 55.0%
CGATGTGGAAATCTGCCACC+AGG + chr1_4:74553067-74553086 None:intergenic 55.0%
TATCCCCCTCCAGATCGATG+TGG + chr1_4:74553082-74553101 None:intergenic 55.0%
GGTCGTGAGATTCGAGAGGG+AGG + chr1_4:74551416-74551435 None:intergenic 60.0%
! GAGCGGTGGTGGTTGGAGAT+CGG + chr1_4:74551437-74551456 None:intergenic 60.0%
AGATCGTCGTCAGGAGGAGG+TGG + chr1_4:74551459-74551478 None:intergenic 60.0%
GGTTGGGGTTAGGTGGAGGT+GGG + chr1_4:74551482-74551501 None:intergenic 60.0%
AATCGCGACGACGAGGGTTG+GGG + chr1_4:74551497-74551516 None:intergenic 60.0%
CGGTCAAAATCGCGACGACG+AGG + chr1_4:74551504-74551523 None:intergenic 60.0%
GGAGGAGAACGACGAGACTG+CGG + chr1_4:74551678-74551697 None:intergenic 60.0%
TTCGGTGGCTATGAACGGGG+AGG - chr1_4:74553013-74553032 Msa0184950:exon 60.0%
GCTATGAACGGGGAGGTGGA+AGG - chr1_4:74553020-74553039 Msa0184950:exon 60.0%
GGTCGTGAGATTCGAGAGGG+AGG + chr1_4:74551416-74551435 None:intergenic 60.0%
! GAGCGGTGGTGGTTGGAGAT+CGG + chr1_4:74551437-74551456 None:intergenic 60.0%
AGATCGTCGTCAGGAGGAGG+TGG + chr1_4:74551459-74551478 None:intergenic 60.0%
GGTTGGGGTTAGGTGGAGGT+GGG + chr1_4:74551482-74551501 None:intergenic 60.0%
AATCGCGACGACGAGGGTTG+GGG + chr1_4:74551497-74551516 None:intergenic 60.0%
CGGTCAAAATCGCGACGACG+AGG + chr1_4:74551504-74551523 None:intergenic 60.0%
GGAGGAGAACGACGAGACTG+CGG + chr1_4:74551678-74551697 None:intergenic 60.0%
TTCGGTGGCTATGAACGGGG+AGG - chr1_4:74553013-74553032 Msa0184950:exon 60.0%
GCTATGAACGGGGAGGTGGA+AGG - chr1_4:74553020-74553039 Msa0184950:exon 60.0%
GTCGTCAGGAGGAGGTGGAG+CGG + chr1_4:74551454-74551473 None:intergenic 65.0%
GGGAGATCGTCGTCAGGAGG+AGG + chr1_4:74551462-74551481 None:intergenic 65.0%
GGTGGGAGATCGTCGTCAGG+AGG + chr1_4:74551465-74551484 None:intergenic 65.0%
GGAGGTGGGAGATCGTCGTC+AGG + chr1_4:74551468-74551487 None:intergenic 65.0%
GGGTTGGGGTTAGGTGGAGG+TGG + chr1_4:74551483-74551502 None:intergenic 65.0%
CGAGGGTTGGGGTTAGGTGG+AGG + chr1_4:74551486-74551505 None:intergenic 65.0%
CGACGAGGGTTGGGGTTAGG+TGG + chr1_4:74551489-74551508 None:intergenic 65.0%
CGACGACGAGGGTTGGGGTT+AGG + chr1_4:74551492-74551511 None:intergenic 65.0%
GCGGAGGAGGAGAATAGCGG+CGG + chr1_4:74551656-74551675 None:intergenic 65.0%
GCGGCGGAGGAGGAGAATAG+CGG + chr1_4:74551659-74551678 None:intergenic 65.0%
GGAGAACGACGAGACTGCGG+CGG + chr1_4:74551675-74551694 None:intergenic 65.0%
GGTGGCTATGAACGGGGAGG+TGG - chr1_4:74553016-74553035 Msa0184950:exon 65.0%
GTCGTCAGGAGGAGGTGGAG+CGG + chr1_4:74551454-74551473 None:intergenic 65.0%
GGGAGATCGTCGTCAGGAGG+AGG + chr1_4:74551462-74551481 None:intergenic 65.0%
GGTGGGAGATCGTCGTCAGG+AGG + chr1_4:74551465-74551484 None:intergenic 65.0%
GGAGGTGGGAGATCGTCGTC+AGG + chr1_4:74551468-74551487 None:intergenic 65.0%
GGGTTGGGGTTAGGTGGAGG+TGG + chr1_4:74551483-74551502 None:intergenic 65.0%
CGAGGGTTGGGGTTAGGTGG+AGG + chr1_4:74551486-74551505 None:intergenic 65.0%
CGACGAGGGTTGGGGTTAGG+TGG + chr1_4:74551489-74551508 None:intergenic 65.0%
CGACGACGAGGGTTGGGGTT+AGG + chr1_4:74551492-74551511 None:intergenic 65.0%
GCGGAGGAGGAGAATAGCGG+CGG + chr1_4:74551656-74551675 None:intergenic 65.0%
GCGGCGGAGGAGGAGAATAG+CGG + chr1_4:74551659-74551678 None:intergenic 65.0%
GGAGAACGACGAGACTGCGG+CGG + chr1_4:74551675-74551694 None:intergenic 65.0%
GGTGGCTATGAACGGGGAGG+TGG - chr1_4:74553016-74553035 Msa0184950:exon 65.0%
! GGAGGTGGAGCGGTGGTGGT+TGG + chr1_4:74551444-74551463 None:intergenic 70.0%
AGGAGGAGGTGGAGCGGTGG+TGG + chr1_4:74551448-74551467 None:intergenic 70.0%
GTCAGGAGGAGGTGGAGCGG+TGG + chr1_4:74551451-74551470 None:intergenic 70.0%
TTCGACCGCCGTCCTCACCG+TGG - chr1_4:74551540-74551559 Msa0184950:intron 70.0%
GAACGACGAGACTGCGGCGG+AGG + chr1_4:74551672-74551691 None:intergenic 70.0%
GAACGGGGAGGTGGAAGGGG+TGG - chr1_4:74553025-74553044 Msa0184950:five_prime_UTR 70.0%
! GGAGGTGGAGCGGTGGTGGT+TGG + chr1_4:74551444-74551463 None:intergenic 70.0%
AGGAGGAGGTGGAGCGGTGG+TGG + chr1_4:74551448-74551467 None:intergenic 70.0%
GTCAGGAGGAGGTGGAGCGG+TGG + chr1_4:74551451-74551470 None:intergenic 70.0%
TTCGACCGCCGTCCTCACCG+TGG - chr1_4:74551540-74551559 Msa0184950:intron 70.0%
GAACGACGAGACTGCGGCGG+AGG + chr1_4:74551672-74551691 None:intergenic 70.0%
GAACGGGGAGGTGGAAGGGG+TGG - chr1_4:74553025-74553044 Msa0184950:five_prime_UTR 70.0%
GACCGCCGTCCTCACCGTGG+CGG - chr1_4:74551543-74551562 Msa0184950:intron 75.0%
CTCGGCCGCCACGGTGAGGA+CGG + chr1_4:74551551-74551570 None:intergenic 75.0%
CACCTCCACCGCGGCCACCT+CGG + chr1_4:74551569-74551588 None:intergenic 75.0%
CGACGAGACTGCGGCGGAGG+AGG + chr1_4:74551669-74551688 None:intergenic 75.0%
GACCGCCGTCCTCACCGTGG+CGG - chr1_4:74551543-74551562 Msa0184950:intron 75.0%
CTCGGCCGCCACGGTGAGGA+CGG + chr1_4:74551551-74551570 None:intergenic 75.0%
CACCTCCACCGCGGCCACCT+CGG + chr1_4:74551569-74551588 None:intergenic 75.0%
CGACGAGACTGCGGCGGAGG+AGG + chr1_4:74551669-74551688 None:intergenic 75.0%
! GGCCGCCACGGTGAGGACGG+CGG + chr1_4:74551548-74551567 None:intergenic 80.0%
! CGTCCTCACCGTGGCGGCCG+AGG - chr1_4:74551549-74551568 Msa0184950:intron 80.0%
! CCACCTCGGCCGCCACGGTG+AGG + chr1_4:74551555-74551574 None:intergenic 80.0%
! CCTCACCGTGGCGGCCGAGG+TGG - chr1_4:74551552-74551571 Msa0184950:intron 80.0%
! CGAGGTGGCCGCGGTGGAGG+TGG - chr1_4:74551567-74551586 Msa0184950:intron 80.0%
! GGCCGCCACGGTGAGGACGG+CGG + chr1_4:74551548-74551567 None:intergenic 80.0%
! CGTCCTCACCGTGGCGGCCG+AGG - chr1_4:74551549-74551568 Msa0184950:intron 80.0%
! CCACCTCGGCCGCCACGGTG+AGG + chr1_4:74551555-74551574 None:intergenic 80.0%
! CCTCACCGTGGCGGCCGAGG+TGG - chr1_4:74551552-74551571 Msa0184950:intron 80.0%
! CGAGGTGGCCGCGGTGGAGG+TGG - chr1_4:74551567-74551586 Msa0184950:intron 80.0%
! CGCGGCCACCTCGGCCGCCA+CGG + chr1_4:74551560-74551579 None:intergenic 85.0%
! CGTGGCGGCCGAGGTGGCCG+CGG - chr1_4:74551558-74551577 Msa0184950:intron 85.0%
! GGCCGAGGTGGCCGCGGTGG+AGG - chr1_4:74551564-74551583 Msa0184950:intron 85.0%
! CGCGGCCACCTCGGCCGCCA+CGG + chr1_4:74551560-74551579 None:intergenic 85.0%
! CGTGGCGGCCGAGGTGGCCG+CGG - chr1_4:74551558-74551577 Msa0184950:intron 85.0%
! GGCCGAGGTGGCCGCGGTGG+AGG - chr1_4:74551564-74551583 Msa0184950:intron 85.0%
! GGCGGCCGAGGTGGCCGCGG+TGG - chr1_4:74551561-74551580 Msa0184950:intron 90.0%
! GGCGGCCGAGGTGGCCGCGG+TGG - chr1_4:74551561-74551580 Msa0184950:intron 90.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 74551263 74553153 74551263 ID=Msa0184950;Name=Msa0184950
chr1_4 mRNA 74551263 74553153 74551263 ID=Msa0184950-mRNA-1;Parent=Msa0184950;Name=Msa0184950-mRNA-1;_AED=0.02;_eAED=0.02;_QI=133|1|0.5|1|1|1|2|0|168
chr1_4 exon 74551263 74551419 74551263 ID=Msa0184950-mRNA-1:exon:25443;Parent=Msa0184950-mRNA-1
chr1_4 exon 74552671 74553153 74552671 ID=Msa0184950-mRNA-1:exon:25442;Parent=Msa0184950-mRNA-1
chr1_4 five_prime_UTR 74553021 74553153 74553021 ID=Msa0184950-mRNA-1:five_prime_utr;Parent=Msa0184950-mRNA-1
chr1_4 CDS 74552671 74553020 74552671 ID=Msa0184950-mRNA-1:cds;Parent=Msa0184950-mRNA-1
chr1_4 CDS 74551263 74551419 74551263 ID=Msa0184950-mRNA-1:cds;Parent=Msa0184950-mRNA-1
Gene Sequence

>Msa0184950

ATGGCCGAAGTCGAAAACCTCCCTCTCGAATCTCACGACCGATCTCCAACCACCACCGCTCCACCTCCTCCTGACGACGATCTCCCACCTCCACCTAACCCCAACCCTCGTCGTCGCGATTTTGACCGTCGTGACGACAGAGATTTCGACCGCCGTCCTCACCGTGGCGGCCGAGGTGGCCGCGGTGGAGGTGGTTACTACAATAACCGTGATAGAGACATCAAACGCCGTCGTAGTCCTAGCCCTAACTATCGTGACCGCCGCTATTCTCCTCCTCCGCCGCAGTCTCGTCGTTCTCCTCCGCCGTATAAGAGATCTAGAAGGGGTAGTCCTCATAGACCTAACGACCGGTTTGGCCATGATCACTTCGGTGGCTATGAACGGGGAGGTGGAAGGGGTGGCTATGCAGATGATAGACCTGGTGGCAGATTTCCACATCGATCTGGAGGGGGATATCAAAACGGAATTTCTGGTATGTCAATAGTTGCAAATAATTTTCAAATATAG
Protein sequence

>Msa0184950

MAEVENLPLESHDRSPTTTAPPPPDDDLPPPPNPNPRRRDFDRRDDRDFDRRPHRGGRGGRGGGGYYNNRDRDIKRRRSPSPNYRDRRYSPPPPQSRRSPPPYKRSRRGSPHRPNDRFGHDHFGGYERGGGRGGYADDRPGGRFPHRSGGGYQNGISGMSIVANNFQI*