AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0185880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0185880 MtrunA17_Chr1g0181581 97.697 304 7 0 6 309 27 330 0.0 620
Msa0185880 MtrunA17_Chr7g0263731 70.395 304 79 5 14 309 29 329 7.30e-132 383
Msa0185880 MtrunA17_Chr1g0200161 50.000 232 105 5 91 312 137 367 8.54e-66 214
Msa0185880 MtrunA17_Chr6g0462191 51.240 242 104 4 42 271 52 291 5.11e-65 211
Msa0185880 MtrunA17_Chr4g0025441 39.216 255 145 5 9 254 2 255 2.34e-35 132
Msa0185880 MtrunA17_Chr4g0045641 33.607 244 143 5 27 256 79 317 4.36e-32 125
Msa0185880 MtrunA17_Chr2g0283321 38.922 167 90 5 94 255 62 221 4.44e-29 113
Msa0185880 MtrunA17_Chr2g0316401 35.260 173 102 3 88 255 67 234 2.36e-27 108
Msa0185880 MtrunA17_Chr3g0135851 42.289 201 93 7 88 268 78 275 8.75e-27 109
Msa0185880 MtrunA17_Chr7g0273861 28.938 273 161 9 3 258 25 281 5.39e-25 104
Msa0185880 MtrunA17_Chr4g0020671 30.242 248 145 8 11 253 30 254 5.61e-25 104
Msa0185880 MtrunA17_Chr8g0346021 29.752 242 143 5 80 302 112 345 3.69e-24 102
Msa0185880 MtrunA17_Chr1g0146931 37.037 189 92 4 94 255 121 309 5.94e-21 93.2
Msa0185880 MtrunA17_Chr3g0126901 37.725 167 90 5 95 253 112 272 3.23e-19 87.8
Msa0185880 MtrunA17_Chr1g0204901 50.000 74 37 0 90 163 131 204 4.95e-16 78.6
Msa0185880 MtrunA17_Chr1g0172611 35.036 137 81 1 90 218 100 236 4.95e-15 75.1
Msa0185880 MtrunA17_Chr8g0389781 53.731 67 31 0 95 161 126 192 7.31e-15 75.1
Msa0185880 MtrunA17_Chr7g0273951 47.297 74 39 0 97 170 108 181 2.57e-14 73.2
Msa0185880 MtrunA17_Chr6g0472551 41.000 100 56 2 75 171 94 193 2.72e-14 73.2
Msa0185880 MtrunA17_Chr2g0330051 41.176 170 88 5 95 254 78 245 2.95e-14 72.8
Msa0185880 MtrunA17_Chr8g0340761 37.931 116 58 4 97 209 128 232 4.72e-14 72.4
Msa0185880 MtrunA17_Chr1g0203711 29.885 174 94 4 94 267 114 259 4.07e-13 69.7
Msa0185880 MtrunA17_Chr2g0330921 51.562 64 31 0 96 159 98 161 5.84e-13 68.9
Msa0185880 MtrunA17_Chr4g0060381 35.246 122 59 2 41 159 60 164 7.26e-13 69.3
Msa0185880 MtrunA17_Chr8g0342551 37.500 168 95 3 95 255 90 254 8.32e-13 68.6
Msa0185880 MtrunA17_Chr1g0166431 42.000 100 56 1 95 192 117 216 1.20e-12 68.2
Msa0185880 MtrunA17_Chr1g0156121 29.560 159 90 2 95 253 124 260 2.85e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0185880 AT5G03040.1 50.000 312 131 6 24 315 41 347 1.85e-87 270
Msa0185880 AT5G03040.3 50.000 312 131 6 24 315 41 347 1.85e-87 270
Msa0185880 AT5G03040.2 50.000 312 131 6 24 315 41 347 1.85e-87 270
Msa0185880 AT3G09710.1 54.651 258 99 7 60 302 76 330 6.01e-77 243
Msa0185880 AT3G09710.2 51.838 272 99 7 60 302 76 344 9.34e-74 235
Msa0185880 AT3G52290.1 45.000 280 141 5 12 286 29 300 8.80e-67 216
Msa0185880 AT2G26410.2 41.553 219 109 5 94 295 147 363 5.61e-42 152
Msa0185880 AT2G26410.1 41.553 219 109 5 94 295 147 363 5.61e-42 152
Msa0185880 AT3G22190.3 45.833 192 96 5 66 254 63 249 4.29e-31 121
Msa0185880 AT3G22190.2 45.833 192 96 5 66 254 63 249 4.29e-31 121
Msa0185880 AT3G22190.4 45.833 192 96 5 66 254 63 249 4.29e-31 121
Msa0185880 AT3G22190.1 45.833 192 96 5 66 254 63 249 4.29e-31 121
Msa0185880 AT2G33990.1 37.349 166 97 3 93 255 45 206 3.17e-30 115
Msa0185880 AT3G59690.2 39.024 164 89 4 98 259 172 326 3.03e-29 117
Msa0185880 AT3G59690.1 39.024 164 89 4 98 259 172 326 3.03e-29 117
Msa0185880 AT2G26180.1 45.665 173 80 4 94 255 83 252 8.63e-29 115
Msa0185880 AT3G15050.1 38.235 170 99 4 89 255 44 210 8.56e-28 109
Msa0185880 AT2G43680.1 38.650 163 86 6 92 251 320 471 5.93e-25 105
Msa0185880 AT2G43680.4 38.650 163 86 6 92 251 319 470 6.15e-25 105
Msa0185880 AT2G43680.2 38.650 163 86 6 92 251 319 470 6.15e-25 105
Msa0185880 AT2G43680.3 38.650 163 86 6 92 251 319 470 6.15e-25 105
Msa0185880 AT2G43680.5 38.650 163 86 6 92 251 319 470 6.15e-25 105
Msa0185880 AT5G13460.1 33.939 165 88 4 96 253 112 262 2.50e-24 102
Msa0185880 AT3G49260.3 34.078 179 99 4 94 255 119 295 7.34e-24 101
Msa0185880 AT3G49260.1 34.270 178 99 5 94 255 119 294 9.28e-24 101
Msa0185880 AT3G49260.4 34.270 178 99 5 94 255 119 294 9.28e-24 101
Msa0185880 AT3G49260.2 34.270 178 99 5 94 255 119 294 9.28e-24 101
Msa0185880 AT1G72670.1 36.464 181 106 4 95 267 93 272 1.90e-22 97.4
Msa0185880 AT4G00820.1 35.196 179 94 6 94 255 131 304 3.66e-21 94.4
Msa0185880 AT1G17480.1 35.714 154 87 3 95 246 94 237 1.36e-19 88.6
Msa0185880 AT1G01110.2 26.706 337 188 12 14 316 43 354 2.65e-19 89.0
Msa0185880 AT4G10640.1 29.289 239 121 7 73 275 75 301 7.43e-16 78.2
Msa0185880 AT1G18840.1 42.000 100 48 1 70 159 74 173 7.76e-16 78.6
Msa0185880 AT1G18840.3 42.000 100 48 1 70 159 74 173 7.76e-16 78.6
Msa0185880 AT1G18840.2 42.000 100 48 1 70 159 74 173 7.76e-16 78.6
Msa0185880 AT4G14750.1 44.186 86 48 0 76 161 86 171 1.28e-15 77.4
Msa0185880 AT4G14750.2 44.186 86 48 0 76 161 145 230 1.49e-15 77.4
Msa0185880 AT4G14750.3 44.186 86 48 0 76 161 145 230 1.69e-15 77.4
Msa0185880 AT5G35670.1 32.124 193 110 7 70 254 132 311 5.51e-15 75.9
Msa0185880 AT1G74690.1 32.727 165 82 3 95 255 113 252 2.28e-14 74.3
Msa0185880 AT3G16490.1 33.036 112 69 2 97 202 109 220 1.18e-12 68.6
Msa0185880 AT1G51960.1 32.911 158 92 4 12 158 9 163 1.69e-11 64.7
Msa0185880 AT5G03960.1 33.333 144 64 3 92 218 106 234 1.82e-11 65.1
Msa0185880 AT5G03960.3 33.333 144 64 3 92 218 107 235 1.82e-11 65.1
Msa0185880 AT5G03960.2 33.333 144 64 3 92 218 107 235 1.82e-11 65.1
Msa0185880 AT5G07240.1 50.847 59 29 0 97 155 108 166 4.51e-11 63.9
Msa0185880 AT5G07240.2 50.847 59 29 0 97 155 154 212 5.26e-11 63.5
Msa0185880 AT4G29150.1 36.957 92 58 0 89 180 125 216 6.00e-11 63.5
Msa0185880 AT4G23060.1 36.634 101 63 1 95 194 167 267 6.70e-11 63.5
Msa0185880 AT1G14380.1 36.471 85 54 0 95 179 94 178 8.94e-11 63.2
Msa0185880 AT1G14380.3 36.471 85 54 0 95 179 94 178 8.94e-11 63.2
Msa0185880 AT1G14380.4 36.471 85 54 0 95 179 94 178 8.94e-11 63.2
Msa0185880 AT1G14380.6 36.471 85 54 0 95 179 94 178 9.12e-11 63.2
Msa0185880 AT1G14380.5 36.471 85 54 0 95 179 94 178 9.12e-11 63.2
Msa0185880 AT1G14380.2 36.471 85 54 0 95 179 94 178 9.12e-11 63.2

Find 149 sgRNAs with CRISPR-Local

Find 305 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATATTTAATTTAATTAA+TGG 0.134990 2_1:-457473 Msa0185880:three_prime_UTR
GAAGAGCATTTCGGGCTTTA+AGG 0.138542 2_1:-460487 Msa0185880:CDS
AAGAATATTTGTTGTATATC+TGG 0.154257 2_1:-457344 Msa0185880:three_prime_UTR
ATCTATAAATCCAATGTTTA+TGG 0.198850 2_1:-458467 Msa0185880:CDS
CCAGAACAGTGTAACATAAT+AGG 0.207238 2_1:+457411 None:intergenic
GGAGGTGCTTGATCAGTTTC+TGG 0.216889 2_1:+461010 None:intergenic
AACCTCATCATTCGGTTTAC+TGG 0.224722 2_1:+460823 None:intergenic
ACCACATTGCCCATGATAAT+TGG 0.235229 2_1:-461762 None:intergenic
TCAGGCAAGAAGAGCATTTC+GGG 0.235316 2_1:-460495 Msa0185880:intron
TGATAAGCTACACATAAATT+TGG 0.239726 2_1:+457682 None:intergenic
AGTTAGGTGAACCCGGATTT+TGG 0.244125 2_1:+458260 None:intergenic
TTCAGTTTCTTGACAATTGC+TGG 0.269742 2_1:+458216 None:intergenic
CTGCACACTCGTCATGCTTT+TGG 0.277740 2_1:+458152 None:intergenic
CCTATTATGTTACACTGTTC+TGG 0.283487 2_1:-457411 Msa0185880:three_prime_UTR
TCCAAAGGTGAGCTGTATTA+TGG 0.294106 2_1:-458184 Msa0185880:three_prime_UTR
GAGAGTTTGGATTGGAAGTT+AGG 0.300049 2_1:+458244 None:intergenic
ATTGCTGGCTTGGGAGAGTT+TGG 0.315971 2_1:+458231 None:intergenic
ATTAAGCTGCTTTCTACCTT+TGG 0.327803 2_1:+457854 None:intergenic
TGACCCGCCTCGAACGAATC+TGG 0.328605 2_1:+460366 None:intergenic
AAAGAAACGGCTTAACTTCC+CGG 0.332661 2_1:-457915 Msa0185880:three_prime_UTR
TTTCTTGACAATTGCTGGCT+TGG 0.333380 2_1:+458221 None:intergenic
TCTCCTTCTCCATCAGGCTT+TGG 0.344634 2_1:+458389 None:intergenic
TTCAGGCAAGAAGAGCATTT+CGG 0.346402 2_1:-460496 Msa0185880:intron
TCAAGCCGCTCCAATTGTTC+AGG 0.350981 2_1:-460849 Msa0185880:CDS
CGGAGGAGGCACTCCATTGC+TGG 0.355945 2_1:-458117 Msa0185880:three_prime_UTR
TTCTTGACAATTGCTGGCTT+GGG 0.362204 2_1:+458222 None:intergenic
CTCGTACTTGCTTAGTAACT+TGG 0.364044 2_1:+460094 None:intergenic
GGCTTATTCATTCACTCATC+AGG 0.365734 2_1:-460045 Msa0185880:intron
CAGAACAGTGTAACATAATA+GGG 0.371935 2_1:+457412 None:intergenic
TGTGTAGCTTATCATTTCCT+TGG 0.374153 2_1:-457673 Msa0185880:three_prime_UTR
TCCAACAGCGAGCCAAAATC+CGG 0.381133 2_1:-458272 Msa0185880:CDS
GGATCAAAGAAGAAATGGTT+TGG 0.384554 2_1:-461064 Msa0185880:CDS
AATTGTTCTCCTTCTCCATC+AGG 0.385930 2_1:+458383 None:intergenic
TTCTGTTATCTTCATTCTTG+TGG 0.386704 2_1:-457503 Msa0185880:three_prime_UTR
AATTGTCAAGAAACTGAATA+AGG 0.387705 2_1:-458211 Msa0185880:exon
TTTGTTGTATATCTGGGAAA+TGG 0.394441 2_1:-457337 Msa0185880:three_prime_UTR
CAAGTGGATCAAAGAAGAAA+TGG 0.394536 2_1:-461069 Msa0185880:CDS
AAGCTGCTTTCTACCTTTGG+TGG 0.401382 2_1:+457857 None:intergenic
CTTCTCCATCAGGCTTTGGT+TGG 0.409739 2_1:+458393 None:intergenic
GAACAGGTTGAGAGCACTAA+TGG 0.410177 2_1:-460459 Msa0185880:CDS
TGAATGCCGCCTTGATGATC+TGG 0.413022 2_1:+457883 None:intergenic
CTCCGCTCCATGCAGACTTT+AGG 0.414742 2_1:-460401 Msa0185880:CDS
TTACTGGCAACCTGAACAAT+TGG 0.421900 2_1:+460839 None:intergenic
TGGACTTTGCAACCTGGACT+TGG 0.423630 2_1:+458014 None:intergenic
GATGATCTGGCAGGCGAAGC+CGG 0.424668 2_1:+457896 None:intergenic
GGATCCAACGAATCCTAGTT+GGG 0.426047 2_1:-458446 Msa0185880:CDS
TCTGTTTGAACATCAGAAAC+AGG 0.427072 2_1:+460884 None:intergenic
GAAACAACAGAAAGGTCCTT+TGG 0.427526 2_1:-457944 Msa0185880:intron
AGATCATCAAGGCGGCATTC+AGG 0.428105 2_1:-457881 Msa0185880:three_prime_UTR
AAGAGCATTTCGGGCTTTAA+GGG 0.430354 2_1:-460486 Msa0185880:CDS
CACAAAAGAGCTAGAGATAC+AGG 0.432698 2_1:-460294 Msa0185880:intron
GTAGCTTATCATTTCCTTGG+AGG 0.439196 2_1:-457670 Msa0185880:three_prime_UTR
GAATGATAACAGTCCCAAAT+GGG 0.439513 2_1:+457523 None:intergenic
AGAATATTTGTTGTATATCT+GGG 0.443320 2_1:-457343 Msa0185880:three_prime_UTR
AGAATGATAACAGTCCCAAA+TGG 0.447334 2_1:+457522 None:intergenic
TGATTTGCTGATTTCAGCAC+TGG 0.452185 2_1:+458330 None:intergenic
GTGTGCAGTCGGAGCGTGTT+CGG 0.453061 2_1:-458137 Msa0185880:three_prime_UTR
AGCTGCTTTCTACCTTTGGT+GGG 0.453672 2_1:+457858 None:intergenic
TTAGTTGGCACCATGTAACT+TGG 0.454849 2_1:+458048 None:intergenic
AAATGAGCTGTCAGAAATCC+TGG 0.456946 2_1:-457557 Msa0185880:three_prime_UTR
AGATTCGTTCGAGGCGGGTC+AGG 0.459187 2_1:-460364 Msa0185880:CDS
TCCATAATACAGCTCACCTT+TGG 0.459798 2_1:+458183 None:intergenic
GATCAAAGAAGAAATGGTTT+GGG 0.464945 2_1:-461063 Msa0185880:CDS
TCAGCCTCAGGTGGATGAAA+AGG 0.467361 2_1:+460989 None:intergenic
GACCCGCCTCGAACGAATCT+GGG 0.469265 2_1:+460367 None:intergenic
ATTGCCGCAACCTCATCATT+CGG 0.473059 2_1:+460815 None:intergenic
CATAACAGATTGGGGAGGAA+TGG 0.475348 2_1:-460151 Msa0185880:intron
TGGATCCAACGAATCCTAGT+TGG 0.479559 2_1:-458447 Msa0185880:CDS
ATAACAGATTGGGGAGGAAT+GGG 0.482482 2_1:-460150 Msa0185880:intron
TGGGACCAGTCCAATTATCA+TGG 0.484592 2_1:+461752 None:intergenic
ATGATCTGGCAGGCGAAGCC+GGG 0.489921 2_1:+457897 None:intergenic
ATAATCTCCTCCTCAGCCTC+AGG 0.493239 2_1:+460977 None:intergenic
TTCGTTGGATCCATAAACAT+TGG 0.495801 2_1:+458457 None:intergenic
GTAGTGTTCTCTGCAGCTGA+TGG 0.498156 2_1:+457994 None:intergenic
AGGGCCAGTCGTAAAACGTC+AGG 0.499673 2_1:-460435 Msa0185880:CDS
GCAAAGTACTCCACTAGTAA+TGG 0.501618 2_1:-457773 Msa0185880:three_prime_UTR
AGTCGGAGCGTGTTCGGAGG+AGG 0.502604 2_1:-458131 Msa0185880:three_prime_UTR
GGTACATCAACATCCACATC+TGG 0.515322 2_1:+460905 None:intergenic
CATCCCCAACTAGGATTCGT+TGG 0.524622 2_1:+458442 None:intergenic
GAGGCGGGTCAGGATGTTAG+AGG 0.525201 2_1:-460354 Msa0185880:CDS
ATCCCAGATTCGTTCGAGGC+GGG 0.527771 2_1:-460369 Msa0185880:CDS
CTAGTAATGGAATTATATCA+TGG 0.529725 2_1:-457760 Msa0185880:three_prime_UTR
CCTTTGGAAATGCAAAGAAA+CGG 0.530250 2_1:-457928 Msa0185880:three_prime_UTR
TACCAAGTATCGCCGAAAAG+TGG 0.530850 2_1:+460781 None:intergenic
ATCCTAGTTGGGGATGGAGC+TGG 0.536093 2_1:-458435 Msa0185880:CDS
TATTTGTGTTGCGTGTGGCT+TGG 0.537012 2_1:-457378 Msa0185880:three_prime_UTR
TATTTATTTGCTCACAAATG+TGG 0.537208 2_1:-457607 Msa0185880:three_prime_UTR
AAGAATCACAATAAGAAATG+AGG 0.540548 2_1:+457450 None:intergenic
TGCCGCCTTGATGATCTGGC+AGG 0.546459 2_1:+457887 None:intergenic
CTCACAAATGTGGTTCATGA+TGG 0.546881 2_1:-457597 Msa0185880:three_prime_UTR
AGCTGATGGACTTTGCAACC+TGG 0.547253 2_1:+458008 None:intergenic
TCATCCACCTGAGGCTGAGG+AGG 0.549283 2_1:-460984 Msa0185880:CDS
CAACCAAAGCCTGATGGAGA+AGG 0.551822 2_1:-458392 Msa0185880:CDS
CTTAATGCAGAAATTACTGT+TGG 0.552747 2_1:-457837 Msa0185880:three_prime_UTR
CCGTTTCTTTGCATTTCCAA+AGG 0.555247 2_1:+457928 None:intergenic
GATTGGAAGTTAGGTGAACC+CGG 0.557782 2_1:+458253 None:intergenic
CTAAAGTCTGCATGGAGCGG+AGG 0.560515 2_1:+460402 None:intergenic
TCCATCAGTTCCAAGTTACA+TGG 0.565302 2_1:-458058 Msa0185880:three_prime_UTR
TGCAATCCCAGATTCGTTCG+AGG 0.569562 2_1:-460373 Msa0185880:CDS
TAAACCGAATGATGAGGTTG+CGG 0.570682 2_1:-460819 Msa0185880:CDS
GGCAGCCAACCAAAGCCTGA+TGG 0.570992 2_1:-458398 Msa0185880:CDS
CACATGACCTAAAGTCTGCA+TGG 0.575607 2_1:+460394 None:intergenic
CAACGAATCCTAGTTGGGGA+TGG 0.576539 2_1:-458441 Msa0185880:CDS
ACCATGTAACTTGGAACTGA+TGG 0.577244 2_1:+458057 None:intergenic
AACCAGCTCCATCCCCAACT+AGG 0.580335 2_1:+458433 None:intergenic
TGGCTTGGGAGAGTTTGGAT+TGG 0.582315 2_1:+458236 None:intergenic
AGAGCATTTCGGGCTTTAAG+GGG 0.582500 2_1:-460485 Msa0185880:CDS
ATCTCCTCCTCAGCCTCAGG+TGG 0.583117 2_1:+460980 None:intergenic
TGATATAATTCCATTACTAG+TGG 0.586145 2_1:+457763 None:intergenic
AGTCTGCAAAAGCCAAGTCC+AGG 0.592138 2_1:-458026 Msa0185880:three_prime_UTR
CTTCGCCTGCCAGATCATCA+AGG 0.592606 2_1:-457892 Msa0185880:three_prime_UTR
CCAACAGCGAGCCAAAATCC+GGG 0.594662 2_1:-458271 Msa0185880:CDS
TGCAGTCGGAGCGTGTTCGG+AGG 0.597571 2_1:-458134 Msa0185880:three_prime_UTR
GAGCTGGTTAGAACGTTGGA+TGG 0.602368 2_1:-458419 Msa0185880:CDS
ATTGCCCATGATAATTGGAC+TGG 0.603587 2_1:-461757 None:intergenic
AGGTTGAGAGCACTAATGGA+AGG 0.610005 2_1:-460455 Msa0185880:CDS
GATGGAGCTGGTTAGAACGT+TGG 0.610918 2_1:-458423 Msa0185880:CDS
CATGGAGCGGAGGGTACTAA+CGG 0.611737 2_1:+460412 None:intergenic
AAGCATGACGAGTGTGCAGT+CGG 0.612598 2_1:-458148 Msa0185880:three_prime_UTR
TAAAGTCTGCATGGAGCGGA+GGG 0.613022 2_1:+460403 None:intergenic
GCCTCAGGTGGATGAAAAGG+AGG 0.614504 2_1:+460992 None:intergenic
GACCTAAAGTCTGCATGGAG+CGG 0.617021 2_1:+460399 None:intergenic
AGCAAAGAAAACAACCTCCA+AGG 0.617429 2_1:+457656 None:intergenic
GGGACCAGTCCAATTATCAT+GGG 0.621023 2_1:+461753 None:intergenic
CCTCACTATTGATCCAGCAA+TGG 0.623634 2_1:+458104 None:intergenic
ACTGAATAAGGCTAGTCCAA+AGG 0.624104 2_1:-458199 Msa0185880:exon
ATGATAACAGTCCCAAATGG+GGG 0.625936 2_1:+457525 None:intergenic
GAGCATTTCGGGCTTTAAGG+GGG 0.629409 2_1:-460484 Msa0185880:CDS
CATTGCTGGATCAATAGTGA+GGG 0.633530 2_1:-458103 Msa0185880:three_prime_UTR
TGTTGAAGTTGTACCAGATG+TGG 0.634465 2_1:-460918 Msa0185880:CDS
TATGATATTTGTGTTGCGTG+TGG 0.635604 2_1:-457383 Msa0185880:three_prime_UTR
GCAGAAATTACTGTTGGCAA+TGG 0.641674 2_1:-457831 Msa0185880:three_prime_UTR
TGTTCAAACAGAAACTGTTG+AGG 0.641908 2_1:-460873 Msa0185880:CDS
GGTTGAGAGCACTAATGGAA+GGG 0.642674 2_1:-460454 Msa0185880:CDS
CCATTGCTGGATCAATAGTG+AGG 0.642996 2_1:-458104 Msa0185880:three_prime_UTR
GCGGCATTCAGGCCCACCAA+AGG 0.652957 2_1:-457870 Msa0185880:three_prime_UTR
AATGATAACAGTCCCAAATG+GGG 0.655301 2_1:+457524 None:intergenic
TGATAACAGTCCCAAATGGG+GGG 0.657634 2_1:+457526 None:intergenic
TATGAGAAGAGAAAGAGCAA+TGG 0.661509 2_1:-460066 Msa0185880:CDS
AATCCCAGATTCGTTCGAGG+CGG 0.666163 2_1:-460370 Msa0185880:CDS
GATCCAACGAATCCTAGTTG+GGG 0.666263 2_1:-458445 Msa0185880:CDS
CAAGTTACTAAGCAAGTACG+AGG 0.670194 2_1:-460093 Msa0185880:CDS
TGCCAGTAAACCGAATGATG+AGG 0.671626 2_1:-460825 Msa0185880:CDS
GGCAACCTGAACAATTGGAG+CGG 0.673984 2_1:+460844 None:intergenic
GCTGTTTGATTATATAACTG+AGG 0.680741 2_1:-457804 Msa0185880:three_prime_UTR
TTTCAGCACTGGTAATGCCG+CGG 0.683035 2_1:+458341 None:intergenic
CGCCTGCCAGATCATCAAGG+CGG 0.686449 2_1:-457889 Msa0185880:three_prime_UTR
ATTCTCATAACAGATTGGGG+AGG 0.696674 2_1:-460156 Msa0185880:intron
AATTACTGTTGGCAATGGTG+TGG 0.715956 2_1:-457826 Msa0185880:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGAAATTAATGCAATAATA+TGG - chr2_1:457670-457689 Msa0185880:three_prime_UTR 15.0%
!! AGAAATTAATGCAATAATAT+GGG - chr2_1:457671-457690 Msa0185880:three_prime_UTR 15.0%
!!! AAATATCAATTTTGTAATTG+AGG + chr2_1:458373-458392 None:intergenic 15.0%
!!! TATATTTGAATTTTAGCTTA+TGG - chr2_1:458868-458887 Msa0185880:intron 15.0%
!!! CATAAAAATGTCATTTTTAT+CGG + chr2_1:459380-459399 None:intergenic 15.0%
!!! TCATTTTCTATTTCTTTTTT+AGG + chr2_1:460142-460161 None:intergenic 15.0%
!! TTTAAACATATATGATTTCT+TGG + chr2_1:460371-460390 None:intergenic 15.0%
!!! TAAGTTGAATAAATTGAAGA+GGG + chr2_1:457601-457620 None:intergenic 20.0%
!! GAAATTAATGCAATAATATG+GGG - chr2_1:457672-457691 Msa0185880:three_prime_UTR 20.0%
!!! ATTTTGTTTTGATATCATTC+AGG - chr2_1:458562-458581 Msa0185880:intron 20.0%
!!! TTTTATTCTGAAGTATATCA+GGG + chr2_1:458825-458844 None:intergenic 20.0%
!!! ATTTTATTCTGAAGTATATC+AGG + chr2_1:458826-458845 None:intergenic 20.0%
!!! AAATTTTTGCATATAAAGCA+TGG - chr2_1:459267-459286 Msa0185880:intron 20.0%
!! TATGAAAAAAATAAGTGTCA+TGG - chr2_1:459396-459415 Msa0185880:intron 20.0%
!!! TTAAATCTTGAGTTATTTTG+AGG + chr2_1:459442-459461 None:intergenic 20.0%
!! AATAAAAACAACTTAAAGAG+AGG + chr2_1:459791-459810 None:intergenic 20.0%
!!! TTTCTATTTCTTTTTTAGGT+GGG + chr2_1:460138-460157 None:intergenic 20.0%
!!! TTTTCTATTTCTTTTTTAGG+TGG + chr2_1:460139-460158 None:intergenic 20.0%
!! AAATTATGTTAGCAGAAAAT+AGG + chr2_1:460485-460504 None:intergenic 20.0%
!!! TAGAAGTGTATATTTATCTT+AGG + chr2_1:460557-460576 None:intergenic 20.0%
!! ATCTATAAATCCAATGTTTA+TGG - chr2_1:460608-460627 Msa0185880:intron 20.0%
!!! AAGAATATTTGTTGTATATC+TGG - chr2_1:461731-461750 Msa0185880:intron 20.0%
!!! AGAATATTTGTTGTATATCT+GGG - chr2_1:461732-461751 Msa0185880:intron 20.0%
! ATGTGATTAGATTAGATCTT+GGG + chr2_1:457483-457502 None:intergenic 25.0%
! AATGTGATTAGATTAGATCT+TGG + chr2_1:457484-457503 None:intergenic 25.0%
! AAATGAAGAAAAAAGAAGAG+AGG + chr2_1:457525-457544 None:intergenic 25.0%
!! GTAAGTTGAATAAATTGAAG+AGG + chr2_1:457602-457621 None:intergenic 25.0%
! TTCACATTAGAAAACCAATT+TGG + chr2_1:457707-457726 None:intergenic 25.0%
!! GTCAAATTGTTTTCATACAA+CGG + chr2_1:457862-457881 None:intergenic 25.0%
! TATGTTTAATCTCAATTACC+TGG - chr2_1:457944-457963 Msa0185880:intron 25.0%
! ATGTTTAATCTCAATTACCT+GGG - chr2_1:457945-457964 Msa0185880:intron 25.0%
! ATTCAACACATAATAGTAAG+AGG + chr2_1:458329-458348 None:intergenic 25.0%
! TAAAAGATGTGTATGTTACA+TGG - chr2_1:458430-458449 Msa0185880:CDS 25.0%
! AACAAAATTCAGCTTGAAAA+TGG + chr2_1:458550-458569 None:intergenic 25.0%
!!! GAATTTTAGCTTATGGATTA+TGG - chr2_1:458875-458894 Msa0185880:intron 25.0%
!!! TAAGAAGTTGCATTTGTTTT+AGG - chr2_1:459067-459086 Msa0185880:intron 25.0%
! TTTACACATAGTATAAAGCA+TGG - chr2_1:459185-459204 Msa0185880:intron 25.0%
!! TTTTTGAACTGAAAGATCTA+AGG - chr2_1:459216-459235 Msa0185880:intron 25.0%
! TTTCATGTAATTTACCTTAC+TGG + chr2_1:459250-459269 None:intergenic 25.0%
! ACAAGATTTGACTAACAATT+CGG + chr2_1:459336-459355 None:intergenic 25.0%
! TTGTTAGTCAAATCTTGTTA+TGG - chr2_1:459338-459357 Msa0185880:intron 25.0%
!!! CATTTTGATATTTCATAGCA+TGG + chr2_1:459719-459738 None:intergenic 25.0%
!! GTAGTTTCAGATATATTTTC+CGG + chr2_1:460079-460098 None:intergenic 25.0%
!!! TTCTATTTCTTTTTTAGGTG+GGG + chr2_1:460137-460156 None:intergenic 25.0%
! CACATCTGATATAAAAATTG+TGG + chr2_1:460174-460193 None:intergenic 25.0%
!! AATTTTGTAGATGAAAACTC+AGG + chr2_1:460347-460366 None:intergenic 25.0%
! TATTTATCTTAGGTCAAACT+TGG + chr2_1:460547-460566 None:intergenic 25.0%
! ACTTCTATTTGACAGAAAAA+CGG - chr2_1:460570-460589 Msa0185880:intron 25.0%
! AATTGTCAAGAAACTGAATA+AGG - chr2_1:460864-460883 Msa0185880:CDS 25.0%
! TGATATAATTCCATTACTAG+TGG + chr2_1:461315-461334 None:intergenic 25.0%
!! CTAGTAATGGAATTATATCA+TGG - chr2_1:461315-461334 Msa0185880:intron 25.0%
! TGATAAGCTACACATAAATT+TGG + chr2_1:461396-461415 None:intergenic 25.0%
! TATTTATTTGCTCACAAATG+TGG - chr2_1:461468-461487 Msa0185880:intron 25.0%
! AAGAATCACAATAAGAAATG+AGG + chr2_1:461628-461647 None:intergenic 25.0%
!!! TGTTTGTTTTGTTTAGAGTC+AGG + chr2_1:457743-457762 None:intergenic 30.0%
CTCTAAACAAAACAAACATC+AGG - chr2_1:457745-457764 Msa0185880:three_prime_UTR 30.0%
! CTGATTTTGTAGAATTCAAG+TGG - chr2_1:457990-458009 Msa0185880:three_prime_UTR 30.0%
GATCAAAGAAGAAATGGTTT+GGG - chr2_1:458012-458031 Msa0185880:three_prime_UTR 30.0%
!! TCATGATTCAAACCACTTTT+CGG - chr2_1:458282-458301 Msa0185880:CDS 30.0%
! CATACACATCTTTTATCGTA+CGG + chr2_1:458425-458444 None:intergenic 30.0%
TCCAAATTCTCATAACAGAT+TGG - chr2_1:458914-458933 Msa0185880:intron 30.0%
CCAAATTCTCATAACAGATT+GGG - chr2_1:458915-458934 Msa0185880:intron 30.0%
CAAATTCTCATAACAGATTG+GGG - chr2_1:458916-458935 Msa0185880:intron 30.0%
ATGCAACTTCTTAAGAGAAA+TGG + chr2_1:459060-459079 None:intergenic 30.0%
! AAATGAGAAAAGAGCATGTA+TGG - chr2_1:459104-459123 Msa0185880:intron 30.0%
TTGATGAACTGAAAGATCAA+AGG - chr2_1:459131-459150 Msa0185880:intron 30.0%
CTGAAAGATCAAAGGAAAAA+TGG - chr2_1:459139-459158 Msa0185880:intron 30.0%
TATATCTTCTGATTCACGAT+CGG + chr2_1:459309-459328 None:intergenic 30.0%
GAAAAGTAGTTAGCTATATG+AGG - chr2_1:459483-459502 Msa0185880:intron 30.0%
AAAATGTCTAGCTACTCTTA+AGG - chr2_1:459515-459534 Msa0185880:intron 30.0%
TAACGAATCGGAATTTAATG+TGG - chr2_1:459596-459615 Msa0185880:intron 30.0%
AACGAATCGGAATTTAATGT+GGG - chr2_1:459597-459616 Msa0185880:intron 30.0%
TCCAAAATAGATACACACTT+AGG - chr2_1:459627-459646 Msa0185880:intron 30.0%
! TCCTAAGTGTGTATCTATTT+TGG + chr2_1:459631-459650 None:intergenic 30.0%
TTCTGTATCAAATCGAGATA+CGG - chr2_1:459666-459685 Msa0185880:intron 30.0%
ATATCAAAATGAGAATCCTG+AGG - chr2_1:459728-459747 Msa0185880:intron 30.0%
TATCAAAATGAGAATCCTGA+GGG - chr2_1:459729-459748 Msa0185880:intron 30.0%
AAAAACAACTTAAAGAGAGG+AGG + chr2_1:459788-459807 None:intergenic 30.0%
ACACACAAGCTTAATAACTT+TGG - chr2_1:459818-459837 Msa0185880:intron 30.0%
CACACAAGCTTAATAACTTT+GGG - chr2_1:459819-459838 Msa0185880:intron 30.0%
!! CTTTTGAGAATTACAGAAAG+TGG - chr2_1:459962-459981 Msa0185880:intron 30.0%
TCATGATTCATTAGCAAAAC+TGG + chr2_1:460042-460061 None:intergenic 30.0%
CTTACAAAATTAATCGACGT+GGG + chr2_1:460319-460338 None:intergenic 30.0%
AAAGATCAAGAAACTCAAAC+TGG + chr2_1:460509-460528 None:intergenic 30.0%
CTTCTATTTGACAGAAAAAC+GGG - chr2_1:460571-460590 Msa0185880:intron 30.0%
CTTAATGCAGAAATTACTGT+TGG - chr2_1:461238-461257 Msa0185880:intron 30.0%
GCTGTTTGATTATATAACTG+AGG - chr2_1:461271-461290 Msa0185880:intron 30.0%
TTCTGTTATCTTCATTCTTG+TGG - chr2_1:461572-461591 Msa0185880:intron 30.0%
CAGAACAGTGTAACATAATA+GGG + chr2_1:461666-461685 None:intergenic 30.0%
! TTTGTTGTATATCTGGGAAA+TGG - chr2_1:461738-461757 Msa0185880:intron 30.0%
AAGAAGAGAGAGAAAGAAGT+GGG + chr2_1:457404-457423 None:intergenic 35.0%
AAAGAAGAGAGAGAAAGAAG+TGG + chr2_1:457405-457424 None:intergenic 35.0%
GAGAGGTTGAAGATAAAAGA+GGG + chr2_1:457508-457527 None:intergenic 35.0%
AGAGAGGTTGAAGATAAAAG+AGG + chr2_1:457509-457528 None:intergenic 35.0%
TCTCTCTTGCATTTCACTTT+AGG - chr2_1:457563-457582 Msa0185880:three_prime_UTR 35.0%
TAGTTTATACATACGCACTG+AGG - chr2_1:457907-457926 Msa0185880:three_prime_UTR 35.0%
AACACATTAAGAAACTTCCC+AGG + chr2_1:457965-457984 None:intergenic 35.0%
CAAGTGGATCAAAGAAGAAA+TGG - chr2_1:458006-458025 Msa0185880:three_prime_UTR 35.0%
GGATCAAAGAAGAAATGGTT+TGG - chr2_1:458011-458030 Msa0185880:three_prime_UTR 35.0%
TCTGTTTGAACATCAGAAAC+AGG + chr2_1:458194-458213 None:intergenic 35.0%
TGTTCAAACAGAAACTGTTG+AGG - chr2_1:458202-458221 Msa0185880:exon 35.0%
ATAACTTGGCATCGTTAAAG+AGG + chr2_1:458467-458486 None:intergenic 35.0%
TAATCGACAATTGCTGCAAA+AGG - chr2_1:458501-458520 Msa0185880:intron 35.0%
CCCAATCTGTTATGAGAATT+TGG + chr2_1:458918-458937 None:intergenic 35.0%
TATGAGAAGAGAAAGAGCAA+TGG - chr2_1:459009-459028 Msa0185880:intron 35.0%
!!! GCATTTGTTTTAGGTTAGTC+AGG - chr2_1:459076-459095 Msa0185880:intron 35.0%
CAAAGGAAAAATGGCTAGTA+AGG - chr2_1:459148-459167 Msa0185880:intron 35.0%
CTAAGGAAAAATGACCAGTA+AGG - chr2_1:459233-459252 Msa0185880:intron 35.0%
GGGAGAAAATCAAACTTAAG+AGG + chr2_1:459889-459908 None:intergenic 35.0%
! TAGCAAAACTGGTTCATACT+AGG + chr2_1:460031-460050 None:intergenic 35.0%
! TTCAGATATATTTTCCGGTC+AGG + chr2_1:460074-460093 None:intergenic 35.0%
!!! TTTCTTTTTTAGGTGGGGTA+GGG + chr2_1:460132-460151 None:intergenic 35.0%
!!! ATTTCTTTTTTAGGTGGGGT+AGG + chr2_1:460133-460152 None:intergenic 35.0%
ACAAATCCTCTAGTCTACTT+TGG - chr2_1:460196-460215 Msa0185880:intron 35.0%
GCTTACAAAATTAATCGACG+TGG + chr2_1:460320-460339 None:intergenic 35.0%
TTCGTTGGATCCATAAACAT+TGG + chr2_1:460621-460640 None:intergenic 35.0%
! AATGATTTTCGGAATTGAGC+TGG + chr2_1:460782-460801 None:intergenic 35.0%
TTCAGTTTCTTGACAATTGC+TGG + chr2_1:460862-460881 None:intergenic 35.0%
AACACTACACCAGAAAAAAG+AGG - chr2_1:461096-461115 Msa0185880:intron 35.0%
ACACTACACCAGAAAAAAGA+GGG - chr2_1:461097-461116 Msa0185880:intron 35.0%
! CCTTTGGAAATGCAAAGAAA+CGG - chr2_1:461147-461166 Msa0185880:intron 35.0%
! ATTAAGCTGCTTTCTACCTT+TGG + chr2_1:461224-461243 None:intergenic 35.0%
TGTGTAGCTTATCATTTCCT+TGG - chr2_1:461402-461421 Msa0185880:intron 35.0%
AATGATAACAGTCCCAAATG+GGG + chr2_1:461554-461573 None:intergenic 35.0%
GAATGATAACAGTCCCAAAT+GGG + chr2_1:461555-461574 None:intergenic 35.0%
AGAATGATAACAGTCCCAAA+TGG + chr2_1:461556-461575 None:intergenic 35.0%
CCAGAACAGTGTAACATAAT+AGG + chr2_1:461667-461686 None:intergenic 35.0%
CCTATTATGTTACACTGTTC+TGG - chr2_1:461664-461683 Msa0185880:intron 35.0%
TATGATATTTGTGTTGCGTG+TGG - chr2_1:461692-461711 Msa0185880:intron 35.0%
AGAAGAGAGAGAAAGAAGTG+GGG + chr2_1:457403-457422 None:intergenic 40.0%
TGGGGAAGAATAAGCCAAAT+TGG - chr2_1:457690-457709 Msa0185880:three_prime_UTR 40.0%
TCAAACATTCTCACAAGCGT+CGG + chr2_1:457808-457827 None:intergenic 40.0%
TGTTGAAGTTGTACCAGATG+TGG - chr2_1:458157-458176 Msa0185880:three_prime_UTR 40.0%
TTACTGGCAACCTGAACAAT+TGG + chr2_1:458239-458258 None:intergenic 40.0%
AACCTCATCATTCGGTTTAC+TGG + chr2_1:458255-458274 None:intergenic 40.0%
TAAACCGAATGATGAGGTTG+CGG - chr2_1:458256-458275 Msa0185880:CDS 40.0%
CCACTAATGACAGCATAACT+TGG + chr2_1:458481-458500 None:intergenic 40.0%
CCAAGTTATGCTGTCATTAG+TGG - chr2_1:458478-458497 Msa0185880:CDS 40.0%
!! TTCAGGCAAGAAGAGCATTT+CGG - chr2_1:458579-458598 Msa0185880:intron 40.0%
!! AAGAGCATTTCGGGCTTTAA+GGG - chr2_1:458589-458608 Msa0185880:intron 40.0%
CACAAAAGAGCTAGAGATAC+AGG - chr2_1:458781-458800 Msa0185880:intron 40.0%
TAGAGATACAGGTCTCTGAA+GGG - chr2_1:458792-458811 Msa0185880:intron 40.0%
ATTCTCATAACAGATTGGGG+AGG - chr2_1:458919-458938 Msa0185880:intron 40.0%
ATAACAGATTGGGGAGGAAT+GGG - chr2_1:458925-458944 Msa0185880:intron 40.0%
CTCGTACTTGCTTAGTAACT+TGG + chr2_1:458984-459003 None:intergenic 40.0%
CAAGTTACTAAGCAAGTACG+AGG - chr2_1:458982-459001 Msa0185880:intron 40.0%
GGCTTATTCATTCACTCATC+AGG - chr2_1:459030-459049 Msa0185880:intron 40.0%
GTCTAGCTACTCTTAAGGTT+AGG - chr2_1:459520-459539 Msa0185880:intron 40.0%
TGAGATTTATCGCAGTGAAC+CGG - chr2_1:459564-459583 Msa0185880:intron 40.0%
GATTTGATACAGAACCCACA+CGG + chr2_1:459660-459679 None:intergenic 40.0%
ATAGTGTGACAATAACCCTC+AGG + chr2_1:459747-459766 None:intergenic 40.0%
TACAGAACACATCAGGGAAA+AGG + chr2_1:459924-459943 None:intergenic 40.0%
CTTCGTTACAGAACACATCA+GGG + chr2_1:459930-459949 None:intergenic 40.0%
ACTTCGTTACAGAACACATC+AGG + chr2_1:459931-459950 None:intergenic 40.0%
!!! TTCTTTTTTAGGTGGGGTAG+GGG + chr2_1:460131-460150 None:intergenic 40.0%
CATGAACCAAAGTAGACTAG+AGG + chr2_1:460205-460224 None:intergenic 40.0%
! CTCTAGTCTACTTTGGTTCA+TGG - chr2_1:460203-460222 Msa0185880:intron 40.0%
CTATTTGACAGAAAAACGGG+CGG - chr2_1:460574-460593 Msa0185880:intron 40.0%
AATTGTTCTCCTTCTCCATC+AGG + chr2_1:460695-460714 None:intergenic 40.0%
TCTGTAAAAAGTTCGATCCG+CGG - chr2_1:460717-460736 Msa0185880:intron 40.0%
!! TGATTTGCTGATTTCAGCAC+TGG + chr2_1:460748-460767 None:intergenic 40.0%
! CGCTGTTGGAGAATGATTTT+CGG + chr2_1:460793-460812 None:intergenic 40.0%
! GAGAGTTTGGATTGGAAGTT+AGG + chr2_1:460834-460853 None:intergenic 40.0%
!! TTCTTGACAATTGCTGGCTT+GGG + chr2_1:460856-460875 None:intergenic 40.0%
! TTTCTTGACAATTGCTGGCT+TGG + chr2_1:460857-460876 None:intergenic 40.0%
! ACTGAATAAGGCTAGTCCAA+AGG - chr2_1:460876-460895 Msa0185880:CDS 40.0%
TCCAAAGGTGAGCTGTATTA+TGG - chr2_1:460891-460910 Msa0185880:CDS 40.0%
TCCATAATACAGCTCACCTT+TGG + chr2_1:460895-460914 None:intergenic 40.0%
CATTGCTGGATCAATAGTGA+GGG - chr2_1:460972-460991 Msa0185880:CDS 40.0%
TCCATCAGTTCCAAGTTACA+TGG - chr2_1:461017-461036 Msa0185880:CDS 40.0%
ACCATGTAACTTGGAACTGA+TGG + chr2_1:461021-461040 None:intergenic 40.0%
TTAGTTGGCACCATGTAACT+TGG + chr2_1:461030-461049 None:intergenic 40.0%
!! TTGGCTTTTGCAGACTTAGT+TGG + chr2_1:461045-461064 None:intergenic 40.0%
TACACCAGAAAAAAGAGGGA+AGG - chr2_1:461101-461120 Msa0185880:intron 40.0%
! AAAAAAGAGGGAAGGCGTAA+TGG - chr2_1:461109-461128 Msa0185880:intron 40.0%
! GCGTAATGGAAACAACAGAA+AGG - chr2_1:461123-461142 Msa0185880:intron 40.0%
GAAACAACAGAAAGGTCCTT+TGG - chr2_1:461131-461150 Msa0185880:intron 40.0%
CCGTTTCTTTGCATTTCCAA+AGG + chr2_1:461150-461169 None:intergenic 40.0%
AAAGAAACGGCTTAACTTCC+CGG - chr2_1:461160-461179 Msa0185880:intron 40.0%
GCAGAAATTACTGTTGGCAA+TGG - chr2_1:461244-461263 Msa0185880:intron 40.0%
AATTACTGTTGGCAATGGTG+TGG - chr2_1:461249-461268 Msa0185880:intron 40.0%
! GCAAAGTACTCCACTAGTAA+TGG - chr2_1:461302-461321 Msa0185880:intron 40.0%
GTAGCTTATCATTTCCTTGG+AGG - chr2_1:461405-461424 Msa0185880:intron 40.0%
AGCAAAGAAAACAACCTCCA+AGG + chr2_1:461422-461441 None:intergenic 40.0%
CTCACAAATGTGGTTCATGA+TGG - chr2_1:461478-461497 Msa0185880:intron 40.0%
AAATGAGCTGTCAGAAATCC+TGG - chr2_1:461518-461537 Msa0185880:intron 40.0%
ATGATAACAGTCCCAAATGG+GGG + chr2_1:461553-461572 None:intergenic 40.0%
TGTTCTGTGATGAGCAGTGT+GGG + chr2_1:457345-457364 None:intergenic 45.0%
GATCTTGGGAAGAGACAAAG+TGG + chr2_1:457469-457488 None:intergenic 45.0%
GGTACATCAACATCCACATC+TGG + chr2_1:458173-458192 None:intergenic 45.0%
TGCCAGTAAACCGAATGATG+AGG - chr2_1:458250-458269 Msa0185880:CDS 45.0%
ATTGCCGCAACCTCATCATT+CGG + chr2_1:458263-458282 None:intergenic 45.0%
!! AACCACTTTTCGGCGATACT+TGG - chr2_1:458292-458311 Msa0185880:CDS 45.0%
! TACCAAGTATCGCCGAAAAG+TGG + chr2_1:458297-458316 None:intergenic 45.0%
!! TCAGGCAAGAAGAGCATTTC+GGG - chr2_1:458580-458599 Msa0185880:intron 45.0%
!! GAAGAGCATTTCGGGCTTTA+AGG - chr2_1:458588-458607 Msa0185880:intron 45.0%
!! AGAGCATTTCGGGCTTTAAG+GGG - chr2_1:458590-458609 Msa0185880:intron 45.0%
!! GAACAGGTTGAGAGCACTAA+TGG - chr2_1:458616-458635 Msa0185880:intron 45.0%
!! AGGTTGAGAGCACTAATGGA+AGG - chr2_1:458620-458639 Msa0185880:intron 45.0%
!! GGTTGAGAGCACTAATGGAA+GGG - chr2_1:458621-458640 Msa0185880:intron 45.0%
CACATGACCTAAAGTCTGCA+TGG + chr2_1:458684-458703 None:intergenic 45.0%
CTAGAGATACAGGTCTCTGA+AGG - chr2_1:458791-458810 Msa0185880:intron 45.0%
CATAACAGATTGGGGAGGAA+TGG - chr2_1:458924-458943 Msa0185880:intron 45.0%
GAGATTTATCGCAGTGAACC+GGG - chr2_1:459565-459584 Msa0185880:intron 45.0%
CGATTCGTTATGATGAGTCC+CGG + chr2_1:459586-459605 None:intergenic 45.0%
CGGGACTCATCATAACGAAT+CGG - chr2_1:459584-459603 Msa0185880:intron 45.0%
ACTTAGGATTCATGCCGTGT+GGG - chr2_1:459643-459662 Msa0185880:intron 45.0%
! AAGAGGCATAAGCAGATGCT+AGG + chr2_1:459872-459891 None:intergenic 45.0%
!! AGTTTGATTTTCTCCCTGCC+TGG - chr2_1:459893-459912 Msa0185880:intron 45.0%
ATCAGGGAAAAGGAATCACC+AGG + chr2_1:459914-459933 None:intergenic 45.0%
CGTAGTACCTCTTTCTGATC+TGG - chr2_1:460004-460023 Msa0185880:intron 45.0%
ACTAGGTCCAGATCAGAAAG+AGG + chr2_1:460014-460033 None:intergenic 45.0%
! GATATATTTTCCGGTCAGGC+TGG + chr2_1:460070-460089 None:intergenic 45.0%
GTGAGATTGCCAGATATACC+TGG - chr2_1:460443-460462 Msa0185880:CDS 45.0%
CCAGATATACCTGGTGAACT+CGG - chr2_1:460452-460471 Msa0185880:CDS 45.0%
TGGATCCAACGAATCCTAGT+TGG - chr2_1:460628-460647 Msa0185880:intron 45.0%
GGATCCAACGAATCCTAGTT+GGG - chr2_1:460629-460648 Msa0185880:intron 45.0%
GATCCAACGAATCCTAGTTG+GGG - chr2_1:460630-460649 Msa0185880:intron 45.0%
! AGTTAGGTGAACCCGGATTT+TGG + chr2_1:460818-460837 None:intergenic 45.0%
GATTGGAAGTTAGGTGAACC+CGG + chr2_1:460825-460844 None:intergenic 45.0%
CCTCACTATTGATCCAGCAA+TGG + chr2_1:460974-460993 None:intergenic 45.0%
CCATTGCTGGATCAATAGTG+AGG - chr2_1:460971-460990 Msa0185880:CDS 45.0%
!!! TACGCCTTCCCTCTTTTTTC+TGG + chr2_1:461108-461127 None:intergenic 45.0%
! AGCTGCTTTCTACCTTTGGT+GGG + chr2_1:461220-461239 None:intergenic 45.0%
! AAGCTGCTTTCTACCTTTGG+TGG + chr2_1:461221-461240 None:intergenic 45.0%
TGATAACAGTCCCAAATGGG+GGG + chr2_1:461552-461571 None:intergenic 45.0%
TATTTGTGTTGCGTGTGGCT+TGG - chr2_1:461697-461716 Msa0185880:intron 45.0%
!! TGAATAAATAAAATAAAAAA+TGG + chr2_1:461372-461391 None:intergenic 5.0%
!!! TGTATATTTAATTTAATTAA+TGG - chr2_1:461602-461621 Msa0185880:intron 5.0%
CTGTTCTGTGATGAGCAGTG+TGG + chr2_1:457346-457365 None:intergenic 50.0%
ACTACCTAGGTGAGTGAGTG+AGG + chr2_1:457377-457396 None:intergenic 50.0%
CACTCACTCACCTAGGTAGT+TGG - chr2_1:457377-457396 Msa0185880:three_prime_UTR 50.0%
!! GGAGGTGCTTGATCAGTTTC+TGG + chr2_1:458068-458087 None:intergenic 50.0%
! ACCTCCTTTTCATCCACCTG+AGG - chr2_1:458082-458101 Msa0185880:three_prime_UTR 50.0%
TCAGCCTCAGGTGGATGAAA+AGG + chr2_1:458089-458108 None:intergenic 50.0%
! TTTTCATCCACCTGAGGCTG+AGG - chr2_1:458088-458107 Msa0185880:three_prime_UTR 50.0%
ATAATCTCCTCCTCAGCCTC+AGG + chr2_1:458101-458120 None:intergenic 50.0%
TCAAGCCGCTCCAATTGTTC+AGG - chr2_1:458226-458245 Msa0185880:CDS 50.0%
GGCAACCTGAACAATTGGAG+CGG + chr2_1:458234-458253 None:intergenic 50.0%
!! GAGCATTTCGGGCTTTAAGG+GGG - chr2_1:458591-458610 Msa0185880:intron 50.0%
TAAAGTCTGCATGGAGCGGA+GGG + chr2_1:458675-458694 None:intergenic 50.0%
GACCTAAAGTCTGCATGGAG+CGG + chr2_1:458679-458698 None:intergenic 50.0%
TGCAATCCCAGATTCGTTCG+AGG - chr2_1:458702-458721 Msa0185880:intron 50.0%
AATCCCAGATTCGTTCGAGG+CGG - chr2_1:458705-458724 Msa0185880:intron 50.0%
CACTTAGGATTCATGCCGTG+TGG - chr2_1:459642-459661 Msa0185880:intron 50.0%
GGAAAAGGAATCACCAGGCA+GGG + chr2_1:459909-459928 None:intergenic 50.0%
CATGAGTATTCCAGCCTGAC+CGG - chr2_1:460057-460076 Msa0185880:CDS 50.0%
CCGAGTTCACCAGGTATATC+TGG + chr2_1:460455-460474 None:intergenic 50.0%
GGTAAATCACCGAGTTCACC+AGG + chr2_1:460464-460483 None:intergenic 50.0%
CATCCCCAACTAGGATTCGT+TGG + chr2_1:460636-460655 None:intergenic 50.0%
CAACGAATCCTAGTTGGGGA+TGG - chr2_1:460634-460653 Msa0185880:intron 50.0%
GATGGAGCTGGTTAGAACGT+TGG - chr2_1:460652-460671 Msa0185880:intron 50.0%
GAGCTGGTTAGAACGTTGGA+TGG - chr2_1:460656-460675 Msa0185880:intron 50.0%
! CTTCTCCATCAGGCTTTGGT+TGG + chr2_1:460685-460704 None:intergenic 50.0%
CAACCAAAGCCTGATGGAGA+AGG - chr2_1:460683-460702 Msa0185880:intron 50.0%
TCTCCTTCTCCATCAGGCTT+TGG + chr2_1:460689-460708 None:intergenic 50.0%
!! TTTCAGCACTGGTAATGCCG+CGG + chr2_1:460737-460756 None:intergenic 50.0%
TCCAACAGCGAGCCAAAATC+CGG - chr2_1:460803-460822 Msa0185880:CDS 50.0%
! TGGCTTGGGAGAGTTTGGAT+TGG + chr2_1:460842-460861 None:intergenic 50.0%
!! ATTGCTGGCTTGGGAGAGTT+TGG + chr2_1:460847-460866 None:intergenic 50.0%
! CTGCACACTCGTCATGCTTT+TGG + chr2_1:460926-460945 None:intergenic 50.0%
AAGCATGACGAGTGTGCAGT+CGG - chr2_1:460927-460946 Msa0185880:CDS 50.0%
AGTCTGCAAAAGCCAAGTCC+AGG - chr2_1:461049-461068 Msa0185880:CDS 50.0%
TGGACTTTGCAACCTGGACT+TGG + chr2_1:461064-461083 None:intergenic 50.0%
!! AGCTGATGGACTTTGCAACC+TGG + chr2_1:461070-461089 None:intergenic 50.0%
GTAGTGTTCTCTGCAGCTGA+TGG + chr2_1:461084-461103 None:intergenic 50.0%
! TGAATGCCGCCTTGATGATC+TGG + chr2_1:461195-461214 None:intergenic 50.0%
AGATCATCAAGGCGGCATTC+AGG - chr2_1:461194-461213 Msa0185880:intron 50.0%
TAGGTGAGTGAGTGAGGTGG+TGG + chr2_1:457371-457390 None:intergenic 55.0%
ACCACCTCACTCACTCACCT+AGG - chr2_1:457370-457389 Msa0185880:three_prime_UTR 55.0%
ACCTAGGTGAGTGAGTGAGG+TGG + chr2_1:457374-457393 None:intergenic 55.0%
AGAAGTGGGGCCAACTACCT+AGG + chr2_1:457390-457409 None:intergenic 55.0%
GCCTCAGGTGGATGAAAAGG+AGG + chr2_1:458086-458105 None:intergenic 55.0%
AGGGCCAGTCGTAAAACGTC+AGG - chr2_1:458640-458659 Msa0185880:intron 55.0%
! ACGGCCTGACGTTTTACGAC+TGG + chr2_1:458647-458666 None:intergenic 55.0%
!! CATGGAGCGGAGGGTACTAA+CGG + chr2_1:458666-458685 None:intergenic 55.0%
CTAAAGTCTGCATGGAGCGG+AGG + chr2_1:458676-458695 None:intergenic 55.0%
CTCCGCTCCATGCAGACTTT+AGG - chr2_1:458674-458693 Msa0185880:intron 55.0%
ATCCCAGATTCGTTCGAGGC+GGG - chr2_1:458706-458725 Msa0185880:intron 55.0%
GGGAAAAGGAATCACCAGGC+AGG + chr2_1:459910-459929 None:intergenic 55.0%
ATCCTAGTTGGGGATGGAGC+TGG - chr2_1:460640-460659 Msa0185880:intron 55.0%
AACCAGCTCCATCCCCAACT+AGG + chr2_1:460645-460664 None:intergenic 55.0%
CCAACAGCGAGCCAAAATCC+GGG - chr2_1:460804-460823 Msa0185880:CDS 55.0%
CTTCGCCTGCCAGATCATCA+AGG - chr2_1:461183-461202 Msa0185880:intron 55.0%
AAATGGGGGGAATGCTCTCC+AGG + chr2_1:461539-461558 None:intergenic 55.0%
!! TGGAGAGCATTCCCCCCATT+TGG - chr2_1:461538-461557 Msa0185880:intron 55.0%
!! GGAGAGCATTCCCCCCATTT+GGG - chr2_1:461539-461558 Msa0185880:intron 55.0%
TCATCCACCTGAGGCTGAGG+AGG - chr2_1:458091-458110 Msa0185880:three_prime_UTR 60.0%
ATCTCCTCCTCAGCCTCAGG+TGG + chr2_1:458098-458117 None:intergenic 60.0%
!! GGGCTTTAAGGGGGCTGAAC+AGG - chr2_1:458600-458619 Msa0185880:intron 60.0%
GACCCGCCTCGAACGAATCT+GGG + chr2_1:458711-458730 None:intergenic 60.0%
TGACCCGCCTCGAACGAATC+TGG + chr2_1:458712-458731 None:intergenic 60.0%
AGATTCGTTCGAGGCGGGTC+AGG - chr2_1:458711-458730 Msa0185880:intron 60.0%
GAGGCGGGTCAGGATGTTAG+AGG - chr2_1:458721-458740 Msa0185880:intron 60.0%
GGCAGCCAACCAAAGCCTGA+TGG - chr2_1:460677-460696 Msa0185880:intron 60.0%
!!! CCCGGATTTTGGCTCGCTGT+TGG + chr2_1:460807-460826 None:intergenic 60.0%
GTGTGCAGTCGGAGCGTGTT+CGG - chr2_1:460938-460957 Msa0185880:CDS 60.0%
ATGATCTGGCAGGCGAAGCC+GGG + chr2_1:461181-461200 None:intergenic 60.0%
GATGATCTGGCAGGCGAAGC+CGG + chr2_1:461182-461201 None:intergenic 60.0%
CGCCTGCCAGATCATCAAGG+CGG - chr2_1:461186-461205 Msa0185880:intron 60.0%
! TGCCGCCTTGATGATCTGGC+AGG + chr2_1:461191-461210 None:intergenic 60.0%
TGCAGTCGGAGCGTGTTCGG+AGG - chr2_1:460941-460960 Msa0185880:CDS 65.0%
AGTCGGAGCGTGTTCGGAGG+AGG - chr2_1:460944-460963 Msa0185880:CDS 65.0%
!! CGGAGGAGGCACTCCATTGC+TGG - chr2_1:460958-460977 Msa0185880:CDS 65.0%
GCGGCATTCAGGCCCACCAA+AGG - chr2_1:461205-461224 Msa0185880:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 457325 461772 457325 ID=Msa0185880;Name=Msa0185880
chr2_1 mRNA 457325 461772 457325 ID=Msa0185880-mRNA-1;Parent=Msa0185880;Name=Msa0185880-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.8|0.83|0.83|0.8|0.66|6|859|321
chr2_1 exon 457325 457956 457325 ID=Msa0185880-mRNA-1:exon:56;Parent=Msa0185880-mRNA-1
chr2_1 exon 457986 458512 457986 ID=Msa0185880-mRNA-1:exon:55;Parent=Msa0185880-mRNA-1
chr2_1 exon 460046 460165 460046 ID=Msa0185880-mRNA-1:exon:54;Parent=Msa0185880-mRNA-1
chr2_1 exon 460295 460513 460295 ID=Msa0185880-mRNA-1:exon:53;Parent=Msa0185880-mRNA-1
chr2_1 exon 460784 461095 460784 ID=Msa0185880-mRNA-1:exon:52;Parent=Msa0185880-mRNA-1
chr2_1 exon 461758 461772 461758 ID=Msa0185880-mRNA-1:exon:51;Parent=Msa0185880-mRNA-1
chr2_1 CDS 461758 461772 461758 ID=Msa0185880-mRNA-1:cds;Parent=Msa0185880-mRNA-1
chr2_1 CDS 460784 461095 460784 ID=Msa0185880-mRNA-1:cds;Parent=Msa0185880-mRNA-1
chr2_1 CDS 460295 460513 460295 ID=Msa0185880-mRNA-1:cds;Parent=Msa0185880-mRNA-1
chr2_1 CDS 460046 460165 460046 ID=Msa0185880-mRNA-1:cds;Parent=Msa0185880-mRNA-1
chr2_1 CDS 458213 458512 458213 ID=Msa0185880-mRNA-1:cds;Parent=Msa0185880-mRNA-1
chr2_1 three_prime_UTR 457986 458212 457986 ID=Msa0185880-mRNA-1:three_prime_utr;Parent=Msa0185880-mRNA-1
chr2_1 three_prime_UTR 457325 457956 457325 ID=Msa0185880-mRNA-1:three_prime_utr;Parent=Msa0185880-mRNA-1
Gene Sequence

>Msa0185880

ATGATAATTGGACTGAATTCAAGTGGATCAAAGAAGAAATGGTTTGGGAAGAAAAAATTAAACGATTCAGATTCATATCCAGAAACTGATCAAGCACCTCCTTTTCATCCACCTGAGGCTGAGGAGGAGATTATTTTAACTCATGTTGAGAATCAAAACAATCATGATCATGTTGAAGTTGTACCAGATGTGGATGTTGATGTACCTGTTTCTGATGTTCAAACAGAAACTGTTGAGGTTCAAGCCGCTCCAATTGTTCAGGTTGCCAGTAAACCGAATGATGAGGTTGCGGCAATCATGATTCAAACCACTTTTCGGCGATACTTGGCAAGAAGAGCATTTCGGGCTTTAAGGGGGCTGAACAGGTTGAGAGCACTAATGGAAGGGCCAGTCGTAAAACGTCAGGCCGTTAGTACCCTCCGCTCCATGCAGACTTTAGGTCATGTGCAATCCCAGATTCGTTCGAGGCGGGTCAGGATGTTAGAGGAGACTCAAGCTCTGCAAAAACAGCTCTTACAGAAGCACACAAAAGAGCTAGAGATACAGATTGGGGAGGAATGGGATGAAAGCATACAATCAAGAGAGCAAATTGAAGCCAAGTTACTAAGCAAGTACGAGGCTACTATGAGAAGAGAAAGAGCAATGGCTTATTCATTCACTCATCAGAAAAACGGGCGGAATTCATCGAAATCTATAAATCCAATGTTTATGGATCCAACGAATCCTAGTTGGGGATGGAGCTGGTTAGAACGTTGGATGGCAGCCAACCAAAGCCTGATGGAGAAGGAGAACAATTCATCTGTAAAAAGTTCGATCCGCGGCATTACCAGTGCTGAAATCAGCAAATCATTTGCTCGTTTCCAGCTCAATTCCGAAAATCATTCTCCAACAGCGAGCCAAAATCCGGGTTCACCTAACTTCCAATCCAAACTCTCCCAAGCCAGCAATTGTCAAGAAACTGAATAA
Protein sequence

>Msa0185880

MIIGLNSSGSKKKWFGKKKLNDSDSYPETDQAPPFHPPEAEEEIILTHVENQNNHDHVEVVPDVDVDVPVSDVQTETVEVQAAPIVQVASKPNDEVAAIMIQTTFRRYLARRAFRALRGLNRLRALMEGPVVKRQAVSTLRSMQTLGHVQSQIRSRRVRMLEETQALQKQLLQKHTKELEIQIGEEWDESIQSREQIEAKLLSKYEATMRRERAMAYSFTHQKNGRNSSKSINPMFMDPTNPSWGWSWLERWMAANQSLMEKENNSSVKSSIRGITSAEISKSFARFQLNSENHSPTASQNPGSPNFQSKLSQASNCQETE*