AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0074000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0074000 MtrunA17_Chr1g0181581 98.069 466 9 0 1 466 1 466 0.0 943
Msa0074000 MtrunA17_Chr7g0263731 69.665 478 121 11 1 465 1 467 0.0 583
Msa0074000 MtrunA17_Chr6g0462191 46.055 469 192 14 1 451 1 426 5.24e-95 293
Msa0074000 MtrunA17_Chr1g0200161 37.870 507 246 15 1 463 1 482 3.72e-79 254
Msa0074000 MtrunA17_Chr4g0045641 28.743 501 265 14 1 436 1 474 3.01e-39 149
Msa0074000 MtrunA17_Chr8g0346021 29.948 384 211 11 101 435 112 486 3.26e-35 137
Msa0074000 MtrunA17_Chr4g0025441 40.310 258 138 7 30 275 2 255 3.39e-35 135
Msa0074000 MtrunA17_Chr4g0020671 29.762 420 210 16 1 407 1 348 2.46e-33 130
Msa0074000 MtrunA17_Chr2g0283321 38.922 167 90 5 115 276 62 221 8.23e-29 115
Msa0074000 MtrunA17_Chr2g0316401 35.758 165 96 3 117 276 75 234 3.55e-27 111
Msa0074000 MtrunA17_Chr3g0135851 41.667 204 96 7 109 292 78 278 1.82e-26 111
Msa0074000 MtrunA17_Chr7g0273861 30.169 295 170 11 5 279 3 281 7.63e-26 109
Msa0074000 MtrunA17_Chr3g0126901 25.977 435 250 14 1 426 1 372 5.39e-22 98.2
Msa0074000 MtrunA17_Chr1g0146931 37.037 189 92 4 115 276 121 309 1.02e-20 94.7
Msa0074000 MtrunA17_Chr1g0204901 40.816 98 51 1 111 201 131 228 7.86e-16 79.7
Msa0074000 MtrunA17_Chr1g0203711 27.742 310 172 9 115 410 114 385 7.47e-15 76.6
Msa0074000 MtrunA17_Chr8g0389781 53.731 67 31 0 116 182 126 192 1.46e-14 75.5
Msa0074000 MtrunA17_Chr1g0172611 35.036 137 81 1 111 239 100 236 2.63e-14 74.3
Msa0074000 MtrunA17_Chr7g0273951 47.297 74 39 0 118 191 108 181 4.44e-14 73.6
Msa0074000 MtrunA17_Chr6g0472551 43.529 85 47 1 109 192 109 193 5.65e-14 73.9
Msa0074000 MtrunA17_Chr2g0330051 41.176 170 88 5 116 275 78 245 7.59e-14 73.2
Msa0074000 MtrunA17_Chr8g0340761 37.931 116 58 4 118 230 128 232 8.46e-14 73.2
Msa0074000 MtrunA17_Chr4g0060381 36.066 122 58 2 62 180 60 164 6.00e-13 70.9
Msa0074000 MtrunA17_Chr2g0330921 51.562 64 31 0 117 180 98 161 9.24e-13 69.3
Msa0074000 MtrunA17_Chr1g0166431 42.000 100 56 1 116 213 117 216 1.62e-12 69.3
Msa0074000 MtrunA17_Chr8g0342551 37.500 168 95 3 116 276 90 254 3.50e-12 68.2
Msa0074000 MtrunA17_Chr1g0156121 30.189 159 89 3 116 274 124 260 4.23e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0074000 AT5G03040.1 51.033 484 194 16 1 465 1 460 1.03e-132 391
Msa0074000 AT5G03040.3 51.033 484 194 16 1 465 1 460 1.03e-132 391
Msa0074000 AT5G03040.2 51.033 484 194 16 1 465 1 460 1.03e-132 391
Msa0074000 AT3G52290.1 43.737 471 209 16 1 459 1 427 1.66e-100 308
Msa0074000 AT3G09710.1 46.154 481 202 20 5 459 4 453 1.59e-93 291
Msa0074000 AT3G09710.2 45.051 495 201 21 5 459 4 467 1.30e-90 283
Msa0074000 AT2G26410.2 35.112 356 186 9 115 461 147 466 2.34e-45 166
Msa0074000 AT2G26410.1 35.112 356 186 9 115 461 147 466 2.34e-45 166
Msa0074000 AT3G59690.2 33.025 324 166 11 119 434 172 452 1.07e-33 133
Msa0074000 AT3G59690.1 33.025 324 166 11 119 434 172 452 1.07e-33 133
Msa0074000 AT2G43680.4 30.211 331 180 10 113 436 319 605 9.55e-32 129
Msa0074000 AT2G43680.2 30.211 331 180 10 113 436 319 605 9.55e-32 129
Msa0074000 AT2G43680.3 30.211 331 180 10 113 436 319 605 9.55e-32 129
Msa0074000 AT2G43680.5 30.211 331 180 10 113 436 319 605 9.55e-32 129
Msa0074000 AT2G43680.1 30.211 331 180 10 113 436 320 606 9.86e-32 129
Msa0074000 AT3G22190.3 45.833 192 96 5 87 275 63 249 6.72e-31 124
Msa0074000 AT3G22190.2 45.833 192 96 5 87 275 63 249 6.72e-31 124
Msa0074000 AT3G22190.4 45.833 192 96 5 87 275 63 249 6.72e-31 124
Msa0074000 AT3G22190.1 45.833 192 96 5 87 275 63 249 6.72e-31 124
Msa0074000 AT2G33990.1 37.349 166 97 3 114 276 45 206 5.10e-30 118
Msa0074000 AT4G00820.1 26.160 474 297 13 8 437 11 475 1.11e-28 119
Msa0074000 AT2G26180.1 45.665 173 80 4 115 276 83 252 1.26e-28 117
Msa0074000 AT1G01110.2 27.273 506 262 22 1 437 1 469 2.94e-28 117
Msa0074000 AT3G15050.1 38.235 170 99 4 110 276 44 210 9.65e-28 111
Msa0074000 AT3G49260.1 26.484 438 255 14 1 412 1 397 1.35e-25 109
Msa0074000 AT3G49260.4 26.484 438 255 14 1 412 1 397 1.35e-25 109
Msa0074000 AT3G49260.2 26.484 438 255 14 1 412 1 397 1.35e-25 109
Msa0074000 AT3G49260.3 26.424 439 255 13 1 412 1 398 1.37e-25 109
Msa0074000 AT5G13460.1 30.164 305 135 11 117 411 112 348 7.38e-25 107
Msa0074000 AT1G72670.1 36.464 181 106 4 116 288 93 272 1.23e-21 97.1
Msa0074000 AT4G10640.1 26.471 476 202 20 3 433 4 376 2.98e-19 90.1
Msa0074000 AT1G17480.1 36.364 154 86 4 116 267 94 237 5.78e-19 89.0
Msa0074000 AT4G14750.1 44.186 86 48 0 97 182 86 171 1.01e-15 79.3
Msa0074000 AT1G18840.1 42.000 100 48 1 91 180 74 173 1.16e-15 79.7
Msa0074000 AT1G18840.3 42.000 100 48 1 91 180 74 173 1.16e-15 79.7
Msa0074000 AT1G18840.2 42.000 100 48 1 91 180 74 173 1.16e-15 79.7
Msa0074000 AT4G14750.2 44.186 86 48 0 97 182 145 230 1.38e-15 79.3
Msa0074000 AT4G14750.3 44.186 86 48 0 97 182 145 230 1.69e-15 79.0
Msa0074000 AT5G35670.1 32.124 193 110 7 91 275 132 311 1.46e-14 75.9
Msa0074000 AT1G74690.1 32.727 165 82 3 116 276 113 252 1.85e-14 76.3
Msa0074000 AT3G16490.1 50.000 62 31 0 118 179 109 170 3.22e-12 68.6
Msa0074000 AT1G51960.1 32.911 158 92 4 33 179 9 163 3.29e-11 65.1
Msa0074000 AT5G07240.1 50.847 59 29 0 118 176 108 166 6.70e-11 64.3
Msa0074000 AT5G07240.2 50.847 59 29 0 118 176 154 212 8.36e-11 64.3
Msa0074000 AT5G03960.1 33.333 144 64 3 113 239 106 234 8.82e-11 63.9
Msa0074000 AT5G03960.3 33.333 144 64 3 113 239 107 235 8.93e-11 63.9
Msa0074000 AT5G03960.2 33.333 144 64 3 113 239 107 235 8.93e-11 63.9

Find 160 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATATTTAATTTAATTAA+TGG 0.134990 1_2:+48142528 Msa0074000:three_prime_UTR
GAAGAGCATTTCGGGCTTTA+AGG 0.138542 1_2:+48139533 Msa0074000:CDS
AAGAATATTTGTTGTATATC+TGG 0.154257 1_2:+48142658 Msa0074000:three_prime_UTR
ATCTATAAATCCAATGTTTA+TGG 0.198850 1_2:+48141555 Msa0074000:CDS
CCAGAACAGTGTAACATAAT+AGG 0.207238 1_2:-48142591 None:intergenic
GGAGGTGCTTGATCAGTTTC+TGG 0.216889 1_2:-48139010 None:intergenic
AACCTCATCATTCGGTTTAC+TGG 0.224722 1_2:-48139197 None:intergenic
TCTCTCTTGCATTTCACTTT+AGG 0.230519 1_2:+48138508 Msa0074000:five_prime_UTR
TCAGGCAAGAAGAGCATTTC+GGG 0.235316 1_2:+48139525 Msa0074000:intron
TGATAAGCTACACATAAATT+TGG 0.239726 1_2:-48142318 None:intergenic
AGTTAGGTGAACCCGGATTT+TGG 0.244125 1_2:-48141762 None:intergenic
TAGAAATTAATGCAATAATA+TGG 0.250528 1_2:+48138615 Msa0074000:exon
GAGAACACTACACCAGAAAA+AGG 0.257119 1_2:+48142045 Msa0074000:CDS
TTCACATTAGAAAACCAATT+TGG 0.258381 1_2:-48138649 None:intergenic
TTCAGTTTCTTGACAATTGC+TGG 0.269742 1_2:-48141811 None:intergenic
CTGCACACTCGTCATGCTTT+TGG 0.277740 1_2:-48141875 None:intergenic
CCTATTATGTTACACTGTTC+TGG 0.283487 1_2:+48142591 Msa0074000:three_prime_UTR
TTCTTGACAATTGCTGGCTT+GGG 0.292014 1_2:-48141805 None:intergenic
TCCAAAGGTGAGCTGTATTA+TGG 0.294106 1_2:+48141843 Msa0074000:CDS
TTTGAGTTGGATTGGAAGTT+AGG 0.295442 1_2:-48141778 None:intergenic
TTTAAGCTGCTTTCTACCTT+TGG 0.311790 1_2:-48142144 None:intergenic
TGACCCGCCTCGAACGAATC+TGG 0.328605 1_2:-48139654 None:intergenic
AAAGAAACGGCTTAACTTCC+CGG 0.332661 1_2:+48142083 Msa0074000:CDS
TTTCTTGACAATTGCTGGCT+TGG 0.333380 1_2:-48141806 None:intergenic
TGGGGAAGAATAAGCCAAAT+TGG 0.335409 1_2:+48138635 Msa0074000:CDS
TCTCCTTCTCCATCAGGCTT+TGG 0.344634 1_2:-48141633 None:intergenic
TTCAGGCAAGAAGAGCATTT+CGG 0.346402 1_2:+48139524 Msa0074000:intron
TCAAGCCGCTCCAATTGTTC+AGG 0.350981 1_2:+48139171 Msa0074000:CDS
CGGAGGAGGCACTCCATTGC+TGG 0.355945 1_2:+48141910 Msa0074000:CDS
CTCGTACTTGCTTAGTAACT+TGG 0.364044 1_2:-48139927 None:intergenic
GGCTTATTCATTCACTCATC+AGG 0.365734 1_2:+48139976 Msa0074000:CDS
AGAAATTAATGCAATAATAT+GGG 0.367739 1_2:+48138616 Msa0074000:exon
CAGAACAGTGTAACATAATA+GGG 0.371935 1_2:-48142590 None:intergenic
TGTGTAGCTTATCATTTCCT+TGG 0.374153 1_2:+48142327 Msa0074000:three_prime_UTR
TCCAACAGCGAGCCAAAATC+CGG 0.381133 1_2:+48141750 Msa0074000:CDS
GGATCAAAGAAGAAATGGTT+TGG 0.384554 1_2:+48138956 Msa0074000:CDS
AATTGTTCTCCTTCTCCATC+AGG 0.385930 1_2:-48141639 None:intergenic
TTCTGTTATCTTCATTCTTG+TGG 0.386704 1_2:+48142498 Msa0074000:three_prime_UTR
AATTGTCAAGAAACTGAATA+AGG 0.387705 1_2:+48141816 Msa0074000:CDS
TTTGTTGTATATCTGGGAAA+TGG 0.394441 1_2:+48142665 Msa0074000:three_prime_UTR
CAAGTGGATCAAAGAAGAAA+TGG 0.394536 1_2:+48138951 Msa0074000:CDS
AAGCTGCTTTCTACCTTTGG+TGG 0.401382 1_2:-48142141 None:intergenic
CTTCTCCATCAGGCTTTGGT+TGG 0.409739 1_2:-48141629 None:intergenic
GAACAGGTTGAGAGCACTAA+TGG 0.410177 1_2:+48139561 Msa0074000:CDS
TGAATGCCGCCTTGATGATC+TGG 0.413022 1_2:-48142115 None:intergenic
CTCCGCTCCATGCAGACTTT+AGG 0.414742 1_2:+48139619 Msa0074000:CDS
TGGGAGGCTTTGAGTTGGAT+TGG 0.416717 1_2:-48141786 None:intergenic
TTACTGGCAACCTGAACAAT+TGG 0.421900 1_2:-48139181 None:intergenic
TGGACTTTGCAACCTGGACT+TGG 0.423630 1_2:-48142013 None:intergenic
GATGATCTGGCAGGCGAAGC+CGG 0.424668 1_2:-48142102 None:intergenic
GGATCCAACGAATCCTAGTT+GGG 0.426047 1_2:+48141576 Msa0074000:CDS
TCTGTTTGAACATCAGAAAC+AGG 0.427072 1_2:-48139136 None:intergenic
AGATCATCAAGGCGGCATTC+AGG 0.428105 1_2:+48142117 Msa0074000:CDS
AGCTGCTTTCTACCTTTGGT+GGG 0.428437 1_2:-48142140 None:intergenic
AAGAGCATTTCGGGCTTTAA+GGG 0.430354 1_2:+48139534 Msa0074000:CDS
CACAAAAGAGCTAGAGATAC+AGG 0.432698 1_2:+48139726 Msa0074000:CDS
ATGTGATTAGATTAGATCTT+GGG 0.439093 1_2:-48138425 None:intergenic
GTAGCTTATCATTTCCTTGG+AGG 0.439196 1_2:+48142330 Msa0074000:three_prime_UTR
GAATGATAACAGTCCCAAAT+GGG 0.439513 1_2:-48142478 None:intergenic
AGAATATTTGTTGTATATCT+GGG 0.443320 1_2:+48142659 Msa0074000:three_prime_UTR
AGAATGATAACAGTCCCAAA+TGG 0.447334 1_2:-48142479 None:intergenic
TTGACAATTGCTGGCTTGGG+AGG 0.449771 1_2:-48141802 None:intergenic
TGATTTGCTGATTTCAGCAC+TGG 0.452185 1_2:-48141692 None:intergenic
GTGTGCAGTCGGAGCGTGTT+CGG 0.453061 1_2:+48141890 Msa0074000:CDS
TTAGTTGGCACCATGTAACT+TGG 0.454849 1_2:-48141979 None:intergenic
AAATGAGCTGTCAGAAATCC+TGG 0.456946 1_2:+48142444 Msa0074000:three_prime_UTR
AGATTCGTTCGAGGCGGGTC+AGG 0.459187 1_2:+48139656 Msa0074000:CDS
TCCATAATACAGCTCACCTT+TGG 0.459798 1_2:-48141844 None:intergenic
AATGTGATTAGATTAGATCT+TGG 0.464043 1_2:-48138426 None:intergenic
GATCAAAGAAGAAATGGTTT+GGG 0.464945 1_2:+48138957 Msa0074000:CDS
TCAGCCTCAGGTGGATGAAA+AGG 0.467361 1_2:-48139031 None:intergenic
GACCCGCCTCGAACGAATCT+GGG 0.469265 1_2:-48139653 None:intergenic
ATTGCCGCAACCTCATCATT+CGG 0.473059 1_2:-48139205 None:intergenic
CATAACAGATTGGGGAGGAA+TGG 0.475348 1_2:+48139870 Msa0074000:intron
TGGATCCAACGAATCCTAGT+TGG 0.479559 1_2:+48141575 Msa0074000:CDS
ATAACAGATTGGGGAGGAAT+GGG 0.482482 1_2:+48139871 Msa0074000:intron
ATGATCTGGCAGGCGAAGCC+GGG 0.489921 1_2:-48142101 None:intergenic
ATAATCTCCTCCTCAGCCTC+AGG 0.493239 1_2:-48139043 None:intergenic
TTCGTTGGATCCATAAACAT+TGG 0.495801 1_2:-48141565 None:intergenic
GTAGTGTTCTCTGCAGCTGA+TGG 0.498156 1_2:-48142033 None:intergenic
CTCTAAACAAAACAAACATC+AGG 0.498653 1_2:+48138690 Msa0074000:CDS
AGGGCCAGTCGTAAAACGTC+AGG 0.499673 1_2:+48139585 Msa0074000:CDS
TGGCTTGGGAGGCTTTGAGT+TGG 0.501361 1_2:-48141791 None:intergenic
GCAAAGTACTCCACTAGTAA+TGG 0.501618 1_2:+48142227 Msa0074000:three_prime_UTR
AGTCGGAGCGTGTTCGGAGG+AGG 0.502604 1_2:+48141896 Msa0074000:CDS
GGTACATCAACATCCACATC+TGG 0.515322 1_2:-48139115 None:intergenic
AGAGAGGTTGAAGATAAAAG+AGG 0.519694 1_2:-48138451 None:intergenic
GAGAGGTTGAAGATAAAAGA+GGG 0.522959 1_2:-48138450 None:intergenic
CATCCCCAACTAGGATTCGT+TGG 0.524622 1_2:-48141580 None:intergenic
GAGGCGGGTCAGGATGTTAG+AGG 0.525201 1_2:+48139666 Msa0074000:CDS
CCTTTGGAAATGCAAAGAAA+CGG 0.526599 1_2:+48142070 Msa0074000:CDS
ATCCCAGATTCGTTCGAGGC+GGG 0.527771 1_2:+48139651 Msa0074000:CDS
CTAGTAATGGAATTATATCA+TGG 0.529725 1_2:+48142240 Msa0074000:three_prime_UTR
TACCAAGTATCGCCGAAAAG+TGG 0.530850 1_2:-48139239 None:intergenic
TTAAATGCAGAAATTACTGT+TGG 0.533004 1_2:+48142162 Msa0074000:CDS
ATCCTAGTTGGGGATGGAGC+TGG 0.536093 1_2:+48141587 Msa0074000:CDS
TATTTGTGTTGCGTGTGGCT+TGG 0.537012 1_2:+48142624 Msa0074000:three_prime_UTR
TATTTATTTGCTCACAAATG+TGG 0.537208 1_2:+48142394 Msa0074000:three_prime_UTR
AGAATCACAAATAAGAAATG+AGG 0.543218 1_2:-48142551 None:intergenic
TGCCGCCTTGATGATCTGGC+AGG 0.546459 1_2:-48142111 None:intergenic
CTCACAAATGTGGTTCATGA+TGG 0.546881 1_2:+48142404 Msa0074000:three_prime_UTR
AGCTGATGGACTTTGCAACC+TGG 0.547253 1_2:-48142019 None:intergenic
TCATCCACCTGAGGCTGAGG+AGG 0.549283 1_2:+48139036 Msa0074000:CDS
CAACCAAAGCCTGATGGAGA+AGG 0.551822 1_2:+48141630 Msa0074000:CDS
TAAGTTGAATAAATTGAAGA+GGG 0.552310 1_2:-48138543 None:intergenic
CCGTTTCTTTGCATTTCCAA+AGG 0.555247 1_2:-48142070 None:intergenic
GATTGGAAGTTAGGTGAACC+CGG 0.557782 1_2:-48141769 None:intergenic
CTAAAGTCTGCATGGAGCGG+AGG 0.560515 1_2:-48139618 None:intergenic
TCCATCAGTTCCAAGTTACA+TGG 0.565302 1_2:+48141969 Msa0074000:CDS
TGCAATCCCAGATTCGTTCG+AGG 0.569562 1_2:+48139647 Msa0074000:CDS
TAAACCGAATGATGAGGTTG+CGG 0.570682 1_2:+48139201 Msa0074000:CDS
GGCAGCCAACCAAAGCCTGA+TGG 0.570992 1_2:+48141624 Msa0074000:CDS
CACATGACCTAAAGTCTGCA+TGG 0.575607 1_2:-48139626 None:intergenic
CAACGAATCCTAGTTGGGGA+TGG 0.576539 1_2:+48141581 Msa0074000:CDS
ACCATGTAACTTGGAACTGA+TGG 0.577244 1_2:-48141970 None:intergenic
GTAAGTTGAATAAATTGAAG+AGG 0.577941 1_2:-48138544 None:intergenic
AACCAGCTCCATCCCCAACT+AGG 0.580335 1_2:-48141589 None:intergenic
AGAGCATTTCGGGCTTTAAG+GGG 0.582500 1_2:+48139535 Msa0074000:CDS
ATCTCCTCCTCAGCCTCAGG+TGG 0.583117 1_2:-48139040 None:intergenic
TGATATAATTCCATTACTAG+TGG 0.586145 1_2:-48142237 None:intergenic
AGTCTGCAAAAGCCAAGTCC+AGG 0.592138 1_2:+48142001 Msa0074000:CDS
CTTCGCCTGCCAGATCATCA+AGG 0.592606 1_2:+48142106 Msa0074000:CDS
GAAATTAATGCAATAATATG+GGG 0.593741 1_2:+48138617 Msa0074000:exon
CCAACAGCGAGCCAAAATCC+GGG 0.594662 1_2:+48141751 Msa0074000:CDS
TGCAGTCGGAGCGTGTTCGG+AGG 0.597571 1_2:+48141893 Msa0074000:CDS
GAGCTGGTTAGAACGTTGGA+TGG 0.602368 1_2:+48141603 Msa0074000:CDS
AGGTTGAGAGCACTAATGGA+AGG 0.610005 1_2:+48139565 Msa0074000:CDS
GATGGAGCTGGTTAGAACGT+TGG 0.610918 1_2:+48141599 Msa0074000:CDS
CATGGAGCGGAGGGTACTAA+CGG 0.611737 1_2:-48139608 None:intergenic
AAGCATGACGAGTGTGCAGT+CGG 0.612598 1_2:+48141879 Msa0074000:CDS
TAAAGTCTGCATGGAGCGGA+GGG 0.613022 1_2:-48139617 None:intergenic
GCCTCAGGTGGATGAAAAGG+AGG 0.614504 1_2:-48139028 None:intergenic
GACCTAAAGTCTGCATGGAG+CGG 0.617021 1_2:-48139621 None:intergenic
AGCAAAGAAAACAACCTCCA+AGG 0.617429 1_2:-48142344 None:intergenic
CCTCACTATTGATCCAGCAA+TGG 0.623634 1_2:-48141923 None:intergenic
ACTGAATAAGGCTAGTCCAA+AGG 0.624104 1_2:+48141828 Msa0074000:CDS
CTGTTTGATTATATAACCTG+AGG 0.625685 1_2:+48142196 Msa0074000:exon
ATGATAACAGTCCCAAATGG+GGG 0.625936 1_2:-48142476 None:intergenic
GAGCATTTCGGGCTTTAAGG+GGG 0.629409 1_2:+48139536 Msa0074000:CDS
CATTGCTGGATCAATAGTGA+GGG 0.633530 1_2:+48141924 Msa0074000:CDS
TGTTGAAGTTGTACCAGATG+TGG 0.634465 1_2:+48139102 Msa0074000:CDS
TATGATATTTGTGTTGCGTG+TGG 0.635604 1_2:+48142619 Msa0074000:three_prime_UTR
TGTTCAAACAGAAACTGTTG+AGG 0.641908 1_2:+48139147 Msa0074000:CDS
GGTTGAGAGCACTAATGGAA+GGG 0.642674 1_2:+48139566 Msa0074000:CDS
CCATTGCTGGATCAATAGTG+AGG 0.642996 1_2:+48141923 Msa0074000:CDS
GATCTTGGGAAGAGACAAAG+TGG 0.645105 1_2:-48138411 None:intergenic
GCGGCATTCAGGCCCACCAA+AGG 0.652957 1_2:+48142128 Msa0074000:CDS
AATGATAACAGTCCCAAATG+GGG 0.655301 1_2:-48142477 None:intergenic
GCAGAAATTACTGTTGGCAA+TGG 0.656114 1_2:+48142168 Msa0074000:CDS
TGATAACAGTCCCAAATGGG+GGG 0.657634 1_2:-48142475 None:intergenic
TATGAGAAGAGAAAGAGCAA+TGG 0.661509 1_2:+48139955 Msa0074000:CDS
AATCCCAGATTCGTTCGAGG+CGG 0.666163 1_2:+48139650 Msa0074000:CDS
GATCCAACGAATCCTAGTTG+GGG 0.666263 1_2:+48141577 Msa0074000:CDS
CAAGTTACTAAGCAAGTACG+AGG 0.670194 1_2:+48139928 Msa0074000:CDS
TGCCAGTAAACCGAATGATG+AGG 0.671626 1_2:+48139195 Msa0074000:CDS
GGCAACCTGAACAATTGGAG+CGG 0.673984 1_2:-48139176 None:intergenic
TTTCAGCACTGGTAATGCCG+CGG 0.683035 1_2:-48141681 None:intergenic
CGCCTGCCAGATCATCAAGG+CGG 0.686449 1_2:+48142109 Msa0074000:CDS
ATTCTCATAACAGATTGGGG+AGG 0.696674 1_2:+48139865 Msa0074000:intron
AATTACTGTTGGCAATGGTG+TGG 0.715956 1_2:+48142173 Msa0074000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGAAATTAATGCAATAATA+TGG + chr1_2:48138615-48138634 Msa0074000:exon 15.0%
!! AGAAATTAATGCAATAATAT+GGG + chr1_2:48138616-48138635 Msa0074000:exon 15.0%
!!! AAATATCAATTTTGTAATTG+AGG - chr1_2:48139318-48139337 None:intergenic 15.0%
!!! TATATTTGAATTTTAGCTTA+TGG + chr1_2:48139814-48139833 Msa0074000:intron 15.0%
!!! CATAAAAATGTCATTTTTAT+CGG - chr1_2:48140326-48140345 None:intergenic 15.0%
!!! TCATTTTCTATTTCTTTTTT+AGG - chr1_2:48141089-48141108 None:intergenic 15.0%
!! TTTAAACATATATGATTTCT+TGG - chr1_2:48141318-48141337 None:intergenic 15.0%
!!! TAAGTTGAATAAATTGAAGA+GGG - chr1_2:48138546-48138565 None:intergenic 20.0%
!! GAAATTAATGCAATAATATG+GGG + chr1_2:48138617-48138636 Msa0074000:exon 20.0%
!!! ATTTTGTTTTGATATCATTC+AGG + chr1_2:48139507-48139526 Msa0074000:intron 20.0%
!!! TTTTATTCTGAAGTATATCA+GGG - chr1_2:48139770-48139789 None:intergenic 20.0%
!!! ATTTTATTCTGAAGTATATC+AGG - chr1_2:48139771-48139790 None:intergenic 20.0%
!!! AAATTTTTGCATATAAAGCA+TGG + chr1_2:48140213-48140232 Msa0074000:intron 20.0%
!! TATGAAAAAAATAAGTGTCA+TGG + chr1_2:48140342-48140361 Msa0074000:intron 20.0%
!!! TTAAATCTTGAGTTATTTTG+AGG - chr1_2:48140388-48140407 None:intergenic 20.0%
!! AATAAAAACAACTTAAAGAG+AGG - chr1_2:48140738-48140757 None:intergenic 20.0%
!!! TTTCTATTTCTTTTTTAGGT+GGG - chr1_2:48141085-48141104 None:intergenic 20.0%
!!! TTTTCTATTTCTTTTTTAGG+TGG - chr1_2:48141086-48141105 None:intergenic 20.0%
!! AAATTATGTTAGCAGAAAAT+AGG - chr1_2:48141432-48141451 None:intergenic 20.0%
!!! TAGAAGTGTATATTTATCTT+AGG - chr1_2:48141504-48141523 None:intergenic 20.0%
!! ATCTATAAATCCAATGTTTA+TGG + chr1_2:48141555-48141574 Msa0074000:CDS 20.0%
!!! AAGAATATTTGTTGTATATC+TGG + chr1_2:48142658-48142677 Msa0074000:three_prime_UTR 20.0%
!!! AGAATATTTGTTGTATATCT+GGG + chr1_2:48142659-48142678 Msa0074000:three_prime_UTR 20.0%
! ATGTGATTAGATTAGATCTT+GGG - chr1_2:48138428-48138447 None:intergenic 25.0%
! AATGTGATTAGATTAGATCT+TGG - chr1_2:48138429-48138448 None:intergenic 25.0%
! AAATGAAGAAAAAAGAAGAG+AGG - chr1_2:48138470-48138489 None:intergenic 25.0%
!! GTAAGTTGAATAAATTGAAG+AGG - chr1_2:48138547-48138566 None:intergenic 25.0%
! TTCACATTAGAAAACCAATT+TGG - chr1_2:48138652-48138671 None:intergenic 25.0%
!! GTCAAATTGTTTTCATACAA+CGG - chr1_2:48138807-48138826 None:intergenic 25.0%
! TATGTTTAATCTCAATTACC+TGG + chr1_2:48138889-48138908 Msa0074000:intron 25.0%
! ATGTTTAATCTCAATTACCT+GGG + chr1_2:48138890-48138909 Msa0074000:intron 25.0%
! ATTCAACACATAATAGTAAG+AGG - chr1_2:48139274-48139293 None:intergenic 25.0%
! TAAAAGATGTGTATGTTACA+TGG + chr1_2:48139375-48139394 Msa0074000:intron 25.0%
! AACAAAATTCAGCTTGAAAA+TGG - chr1_2:48139495-48139514 None:intergenic 25.0%
!!! GAATTTTAGCTTATGGATTA+TGG + chr1_2:48139821-48139840 Msa0074000:intron 25.0%
!!! TAAGAAGTTGCATTTGTTTT+AGG + chr1_2:48140013-48140032 Msa0074000:intron 25.0%
! TTTACACATAGTATAAAGCA+TGG + chr1_2:48140131-48140150 Msa0074000:intron 25.0%
!! TTTTTGAACTGAAAGATCTA+AGG + chr1_2:48140162-48140181 Msa0074000:intron 25.0%
! TTTCATGTAATTTACCTTAC+TGG - chr1_2:48140196-48140215 None:intergenic 25.0%
! ACAAGATTTGACTAACAATT+CGG - chr1_2:48140282-48140301 None:intergenic 25.0%
! TTGTTAGTCAAATCTTGTTA+TGG + chr1_2:48140284-48140303 Msa0074000:intron 25.0%
!!! CATTTTGATATTTCATAGCA+TGG - chr1_2:48140665-48140684 None:intergenic 25.0%
!! GTAGTTTCAGATATATTTTC+CGG - chr1_2:48141026-48141045 None:intergenic 25.0%
!!! TTCTATTTCTTTTTTAGGTG+GGG - chr1_2:48141084-48141103 None:intergenic 25.0%
! CACATCTGATATAAAAATTG+TGG - chr1_2:48141121-48141140 None:intergenic 25.0%
!! AATTTTGTAGATGAAAACTC+AGG - chr1_2:48141294-48141313 None:intergenic 25.0%
! TATTTATCTTAGGTCAAACT+TGG - chr1_2:48141494-48141513 None:intergenic 25.0%
! ACTTCTATTTGACAGAAAAA+CGG + chr1_2:48141517-48141536 Msa0074000:intron 25.0%
! AATTGTCAAGAAACTGAATA+AGG + chr1_2:48141816-48141835 Msa0074000:CDS 25.0%
! TTAAATGCAGAAATTACTGT+TGG + chr1_2:48142162-48142181 Msa0074000:CDS 25.0%
! TGATATAATTCCATTACTAG+TGG - chr1_2:48142240-48142259 None:intergenic 25.0%
!! CTAGTAATGGAATTATATCA+TGG + chr1_2:48142240-48142259 Msa0074000:three_prime_UTR 25.0%
! TGATAAGCTACACATAAATT+TGG - chr1_2:48142321-48142340 None:intergenic 25.0%
! TATTTATTTGCTCACAAATG+TGG + chr1_2:48142394-48142413 Msa0074000:three_prime_UTR 25.0%
! AGAATCACAAATAAGAAATG+AGG - chr1_2:48142554-48142573 None:intergenic 25.0%
!!! TGTTTGTTTTGTTTAGAGTC+AGG - chr1_2:48138688-48138707 None:intergenic 30.0%
CTCTAAACAAAACAAACATC+AGG + chr1_2:48138690-48138709 Msa0074000:CDS 30.0%
! CTGATTTTGTAGAATTCAAG+TGG + chr1_2:48138935-48138954 Msa0074000:intron 30.0%
GATCAAAGAAGAAATGGTTT+GGG + chr1_2:48138957-48138976 Msa0074000:CDS 30.0%
!! TCATGATTCAAACCACTTTT+CGG + chr1_2:48139227-48139246 Msa0074000:CDS 30.0%
! CATACACATCTTTTATCGTA+CGG - chr1_2:48139370-48139389 None:intergenic 30.0%
TCCAAATTCTCATAACAGAT+TGG + chr1_2:48139860-48139879 Msa0074000:intron 30.0%
CCAAATTCTCATAACAGATT+GGG + chr1_2:48139861-48139880 Msa0074000:intron 30.0%
CAAATTCTCATAACAGATTG+GGG + chr1_2:48139862-48139881 Msa0074000:intron 30.0%
ATGCAACTTCTTAAGAGAAA+TGG - chr1_2:48140006-48140025 None:intergenic 30.0%
! AAATGAGAAAAGAGCATGTA+TGG + chr1_2:48140050-48140069 Msa0074000:intron 30.0%
TTGATGAACTGAAAGATCAA+AGG + chr1_2:48140077-48140096 Msa0074000:intron 30.0%
CTGAAAGATCAAAGGAAAAA+TGG + chr1_2:48140085-48140104 Msa0074000:intron 30.0%
TATATCTTCTGATTCACGAT+CGG - chr1_2:48140255-48140274 None:intergenic 30.0%
GAAAAGTAGTTAGCTATATG+AGG + chr1_2:48140429-48140448 Msa0074000:intron 30.0%
AAAATGTCTAGCTACTCTTA+AGG + chr1_2:48140461-48140480 Msa0074000:intron 30.0%
TAACGAATCGGAATTTAATG+TGG + chr1_2:48140542-48140561 Msa0074000:intron 30.0%
AACGAATCGGAATTTAATGT+GGG + chr1_2:48140543-48140562 Msa0074000:intron 30.0%
TCCAAAATAGATACACACTT+AGG + chr1_2:48140573-48140592 Msa0074000:intron 30.0%
! TCCTAAGTGTGTATCTATTT+TGG - chr1_2:48140577-48140596 None:intergenic 30.0%
TTCTGTATCAAATCGAGATA+CGG + chr1_2:48140612-48140631 Msa0074000:intron 30.0%
ATATCAAAATGAGAATCCTG+AGG + chr1_2:48140674-48140693 Msa0074000:intron 30.0%
TATCAAAATGAGAATCCTGA+GGG + chr1_2:48140675-48140694 Msa0074000:intron 30.0%
AAAAACAACTTAAAGAGAGG+AGG - chr1_2:48140735-48140754 None:intergenic 30.0%
ACACACAAGCTTAATAACTT+TGG + chr1_2:48140765-48140784 Msa0074000:intron 30.0%
CACACAAGCTTAATAACTTT+GGG + chr1_2:48140766-48140785 Msa0074000:intron 30.0%
!! CTTTTGAGAATTACAGAAAG+TGG + chr1_2:48140909-48140928 Msa0074000:intron 30.0%
TCATGATTCATTAGCAAAAC+TGG - chr1_2:48140989-48141008 None:intergenic 30.0%
CTTACAAAATTAATCGACGT+GGG - chr1_2:48141266-48141285 None:intergenic 30.0%
AAAGATCAAGAAACTCAAAC+TGG - chr1_2:48141456-48141475 None:intergenic 30.0%
CTTCTATTTGACAGAAAAAC+GGG + chr1_2:48141518-48141537 Msa0074000:intron 30.0%
CTGTTTGATTATATAACCTG+AGG + chr1_2:48142196-48142215 Msa0074000:exon 30.0%
TTCTGTTATCTTCATTCTTG+TGG + chr1_2:48142498-48142517 Msa0074000:three_prime_UTR 30.0%
CAGAACAGTGTAACATAATA+GGG - chr1_2:48142593-48142612 None:intergenic 30.0%
! TTTGTTGTATATCTGGGAAA+TGG + chr1_2:48142665-48142684 Msa0074000:three_prime_UTR 30.0%
GAGAGGTTGAAGATAAAAGA+GGG - chr1_2:48138453-48138472 None:intergenic 35.0%
AGAGAGGTTGAAGATAAAAG+AGG - chr1_2:48138454-48138473 None:intergenic 35.0%
TCTCTCTTGCATTTCACTTT+AGG + chr1_2:48138508-48138527 Msa0074000:five_prime_UTR 35.0%
TAGTTTATACATACGCACTG+AGG + chr1_2:48138852-48138871 Msa0074000:intron 35.0%
AACACATTAAGAAACTTCCC+AGG - chr1_2:48138910-48138929 None:intergenic 35.0%
CAAGTGGATCAAAGAAGAAA+TGG + chr1_2:48138951-48138970 Msa0074000:CDS 35.0%
GGATCAAAGAAGAAATGGTT+TGG + chr1_2:48138956-48138975 Msa0074000:CDS 35.0%
TCTGTTTGAACATCAGAAAC+AGG - chr1_2:48139139-48139158 None:intergenic 35.0%
TGTTCAAACAGAAACTGTTG+AGG + chr1_2:48139147-48139166 Msa0074000:CDS 35.0%
ATAACTTGGCATCGTTAAAG+AGG - chr1_2:48139412-48139431 None:intergenic 35.0%
TAATCGACAATTGCTGCAAA+AGG + chr1_2:48139446-48139465 Msa0074000:intron 35.0%
CCCAATCTGTTATGAGAATT+TGG - chr1_2:48139864-48139883 None:intergenic 35.0%
TATGAGAAGAGAAAGAGCAA+TGG + chr1_2:48139955-48139974 Msa0074000:CDS 35.0%
!!! GCATTTGTTTTAGGTTAGTC+AGG + chr1_2:48140022-48140041 Msa0074000:intron 35.0%
CAAAGGAAAAATGGCTAGTA+AGG + chr1_2:48140094-48140113 Msa0074000:intron 35.0%
CTAAGGAAAAATGACCAGTA+AGG + chr1_2:48140179-48140198 Msa0074000:intron 35.0%
GGGAGAAAATCAAACTTAAG+AGG - chr1_2:48140836-48140855 None:intergenic 35.0%
! TAGCAAAACTGGTTCATACT+AGG - chr1_2:48140978-48140997 None:intergenic 35.0%
! TTCAGATATATTTTCCGGTC+AGG - chr1_2:48141021-48141040 None:intergenic 35.0%
!!! TTTCTTTTTTAGGTGGGGTA+GGG - chr1_2:48141079-48141098 None:intergenic 35.0%
!!! ATTTCTTTTTTAGGTGGGGT+AGG - chr1_2:48141080-48141099 None:intergenic 35.0%
ACAAATCCTCTAGTCTACTT+TGG + chr1_2:48141143-48141162 Msa0074000:intron 35.0%
GCTTACAAAATTAATCGACG+TGG - chr1_2:48141267-48141286 None:intergenic 35.0%
TTCGTTGGATCCATAAACAT+TGG - chr1_2:48141568-48141587 None:intergenic 35.0%
! AATGATTTTCGGAATTGAGC+TGG - chr1_2:48141729-48141748 None:intergenic 35.0%
!! TTTGAGTTGGATTGGAAGTT+AGG - chr1_2:48141781-48141800 None:intergenic 35.0%
TTCAGTTTCTTGACAATTGC+TGG - chr1_2:48141814-48141833 None:intergenic 35.0%
AGAACACTACACCAGAAAAA+GGG + chr1_2:48142046-48142065 Msa0074000:CDS 35.0%
! CCTTTGGAAATGCAAAGAAA+CGG + chr1_2:48142070-48142089 Msa0074000:CDS 35.0%
! TTTAAGCTGCTTTCTACCTT+TGG - chr1_2:48142147-48142166 None:intergenic 35.0%
! TACTTTGCATTCTTTTCCTC+AGG - chr1_2:48142215-48142234 None:intergenic 35.0%
TGTGTAGCTTATCATTTCCT+TGG + chr1_2:48142327-48142346 Msa0074000:three_prime_UTR 35.0%
AATGATAACAGTCCCAAATG+GGG - chr1_2:48142480-48142499 None:intergenic 35.0%
GAATGATAACAGTCCCAAAT+GGG - chr1_2:48142481-48142500 None:intergenic 35.0%
AGAATGATAACAGTCCCAAA+TGG - chr1_2:48142482-48142501 None:intergenic 35.0%
CCAGAACAGTGTAACATAAT+AGG - chr1_2:48142594-48142613 None:intergenic 35.0%
CCTATTATGTTACACTGTTC+TGG + chr1_2:48142591-48142610 Msa0074000:three_prime_UTR 35.0%
TATGATATTTGTGTTGCGTG+TGG + chr1_2:48142619-48142638 Msa0074000:three_prime_UTR 35.0%
TGGGGAAGAATAAGCCAAAT+TGG + chr1_2:48138635-48138654 Msa0074000:CDS 40.0%
TCAAACATTCTCACAAGCGT+CGG - chr1_2:48138753-48138772 None:intergenic 40.0%
TGTTGAAGTTGTACCAGATG+TGG + chr1_2:48139102-48139121 Msa0074000:CDS 40.0%
TTACTGGCAACCTGAACAAT+TGG - chr1_2:48139184-48139203 None:intergenic 40.0%
AACCTCATCATTCGGTTTAC+TGG - chr1_2:48139200-48139219 None:intergenic 40.0%
TAAACCGAATGATGAGGTTG+CGG + chr1_2:48139201-48139220 Msa0074000:CDS 40.0%
CCACTAATGACAGCATAACT+TGG - chr1_2:48139426-48139445 None:intergenic 40.0%
CCAAGTTATGCTGTCATTAG+TGG + chr1_2:48139423-48139442 Msa0074000:intron 40.0%
!! TTCAGGCAAGAAGAGCATTT+CGG + chr1_2:48139524-48139543 Msa0074000:intron 40.0%
!! AAGAGCATTTCGGGCTTTAA+GGG + chr1_2:48139534-48139553 Msa0074000:CDS 40.0%
CACAAAAGAGCTAGAGATAC+AGG + chr1_2:48139726-48139745 Msa0074000:CDS 40.0%
TAGAGATACAGGTCTCTGAA+GGG + chr1_2:48139737-48139756 Msa0074000:intron 40.0%
ATTCTCATAACAGATTGGGG+AGG + chr1_2:48139865-48139884 Msa0074000:intron 40.0%
ATAACAGATTGGGGAGGAAT+GGG + chr1_2:48139871-48139890 Msa0074000:intron 40.0%
CTCGTACTTGCTTAGTAACT+TGG - chr1_2:48139930-48139949 None:intergenic 40.0%
CAAGTTACTAAGCAAGTACG+AGG + chr1_2:48139928-48139947 Msa0074000:CDS 40.0%
GGCTTATTCATTCACTCATC+AGG + chr1_2:48139976-48139995 Msa0074000:CDS 40.0%
GTCTAGCTACTCTTAAGGTT+AGG + chr1_2:48140466-48140485 Msa0074000:intron 40.0%
TGAGATTTATCGCAGTGAAC+CGG + chr1_2:48140510-48140529 Msa0074000:intron 40.0%
GATTTGATACAGAACCCACA+CGG - chr1_2:48140606-48140625 None:intergenic 40.0%
ATAGTGTGACAATAACCCTC+AGG - chr1_2:48140693-48140712 None:intergenic 40.0%
TACAGAACACATCAGGGAAA+AGG - chr1_2:48140871-48140890 None:intergenic 40.0%
CTTCGTTACAGAACACATCA+GGG - chr1_2:48140877-48140896 None:intergenic 40.0%
ACTTCGTTACAGAACACATC+AGG - chr1_2:48140878-48140897 None:intergenic 40.0%
!!! TTCTTTTTTAGGTGGGGTAG+GGG - chr1_2:48141078-48141097 None:intergenic 40.0%
CATGAACCAAAGTAGACTAG+AGG - chr1_2:48141152-48141171 None:intergenic 40.0%
! CTCTAGTCTACTTTGGTTCA+TGG + chr1_2:48141150-48141169 Msa0074000:intron 40.0%
CTATTTGACAGAAAAACGGG+CGG + chr1_2:48141521-48141540 Msa0074000:intron 40.0%
AATTGTTCTCCTTCTCCATC+AGG - chr1_2:48141642-48141661 None:intergenic 40.0%
TCTGTAAAAAGTTCGATCCG+CGG + chr1_2:48141664-48141683 Msa0074000:CDS 40.0%
!! TGATTTGCTGATTTCAGCAC+TGG - chr1_2:48141695-48141714 None:intergenic 40.0%
! CGCTGTTGGAGAATGATTTT+CGG - chr1_2:48141740-48141759 None:intergenic 40.0%
!! TTCTTGACAATTGCTGGCTT+GGG - chr1_2:48141808-48141827 None:intergenic 40.0%
! TTTCTTGACAATTGCTGGCT+TGG - chr1_2:48141809-48141828 None:intergenic 40.0%
! ACTGAATAAGGCTAGTCCAA+AGG + chr1_2:48141828-48141847 Msa0074000:CDS 40.0%
TCCAAAGGTGAGCTGTATTA+TGG + chr1_2:48141843-48141862 Msa0074000:CDS 40.0%
TCCATAATACAGCTCACCTT+TGG - chr1_2:48141847-48141866 None:intergenic 40.0%
CATTGCTGGATCAATAGTGA+GGG + chr1_2:48141924-48141943 Msa0074000:CDS 40.0%
TCCATCAGTTCCAAGTTACA+TGG + chr1_2:48141969-48141988 Msa0074000:CDS 40.0%
ACCATGTAACTTGGAACTGA+TGG - chr1_2:48141973-48141992 None:intergenic 40.0%
TTAGTTGGCACCATGTAACT+TGG - chr1_2:48141982-48142001 None:intergenic 40.0%
!! TTGGCTTTTGCAGACTTAGT+TGG - chr1_2:48141997-48142016 None:intergenic 40.0%
GAGAACACTACACCAGAAAA+AGG + chr1_2:48142045-48142064 Msa0074000:CDS 40.0%
! TTTCCAAAGGACCCTTTTTC+TGG - chr1_2:48142060-48142079 None:intergenic 40.0%
CCGTTTCTTTGCATTTCCAA+AGG - chr1_2:48142073-48142092 None:intergenic 40.0%
AAAGAAACGGCTTAACTTCC+CGG + chr1_2:48142083-48142102 Msa0074000:CDS 40.0%
GCAGAAATTACTGTTGGCAA+TGG + chr1_2:48142168-48142187 Msa0074000:CDS 40.0%
AATTACTGTTGGCAATGGTG+TGG + chr1_2:48142173-48142192 Msa0074000:CDS 40.0%
! GCAAAGTACTCCACTAGTAA+TGG + chr1_2:48142227-48142246 Msa0074000:three_prime_UTR 40.0%
GTAGCTTATCATTTCCTTGG+AGG + chr1_2:48142330-48142349 Msa0074000:three_prime_UTR 40.0%
AGCAAAGAAAACAACCTCCA+AGG - chr1_2:48142347-48142366 None:intergenic 40.0%
CTCACAAATGTGGTTCATGA+TGG + chr1_2:48142404-48142423 Msa0074000:three_prime_UTR 40.0%
AAATGAGCTGTCAGAAATCC+TGG + chr1_2:48142444-48142463 Msa0074000:three_prime_UTR 40.0%
ATGATAACAGTCCCAAATGG+GGG - chr1_2:48142479-48142498 None:intergenic 40.0%
GATCTTGGGAAGAGACAAAG+TGG - chr1_2:48138414-48138433 None:intergenic 45.0%
GGTACATCAACATCCACATC+TGG - chr1_2:48139118-48139137 None:intergenic 45.0%
TGCCAGTAAACCGAATGATG+AGG + chr1_2:48139195-48139214 Msa0074000:CDS 45.0%
ATTGCCGCAACCTCATCATT+CGG - chr1_2:48139208-48139227 None:intergenic 45.0%
!! AACCACTTTTCGGCGATACT+TGG + chr1_2:48139237-48139256 Msa0074000:CDS 45.0%
! TACCAAGTATCGCCGAAAAG+TGG - chr1_2:48139242-48139261 None:intergenic 45.0%
!! TCAGGCAAGAAGAGCATTTC+GGG + chr1_2:48139525-48139544 Msa0074000:intron 45.0%
!! GAAGAGCATTTCGGGCTTTA+AGG + chr1_2:48139533-48139552 Msa0074000:CDS 45.0%
!! AGAGCATTTCGGGCTTTAAG+GGG + chr1_2:48139535-48139554 Msa0074000:CDS 45.0%
!! GAACAGGTTGAGAGCACTAA+TGG + chr1_2:48139561-48139580 Msa0074000:CDS 45.0%
!! AGGTTGAGAGCACTAATGGA+AGG + chr1_2:48139565-48139584 Msa0074000:CDS 45.0%
!! GGTTGAGAGCACTAATGGAA+GGG + chr1_2:48139566-48139585 Msa0074000:CDS 45.0%
CACATGACCTAAAGTCTGCA+TGG - chr1_2:48139629-48139648 None:intergenic 45.0%
CTAGAGATACAGGTCTCTGA+AGG + chr1_2:48139736-48139755 Msa0074000:intron 45.0%
CATAACAGATTGGGGAGGAA+TGG + chr1_2:48139870-48139889 Msa0074000:intron 45.0%
GAGATTTATCGCAGTGAACC+GGG + chr1_2:48140511-48140530 Msa0074000:intron 45.0%
CGATTCGTTATGATGAGTCC+CGG - chr1_2:48140532-48140551 None:intergenic 45.0%
CGGGACTCATCATAACGAAT+CGG + chr1_2:48140530-48140549 Msa0074000:intron 45.0%
ACTTAGGATTCATGCCGTGT+GGG + chr1_2:48140589-48140608 Msa0074000:intron 45.0%
! AAGAGGCATAAGCAGATGCT+AGG - chr1_2:48140819-48140838 None:intergenic 45.0%
!! AGTTTGATTTTCTCCCTGCC+TGG + chr1_2:48140840-48140859 Msa0074000:intron 45.0%
ATCAGGGAAAAGGAATCACC+AGG - chr1_2:48140861-48140880 None:intergenic 45.0%
CGTAGTACCTCTTTCTGATC+TGG + chr1_2:48140951-48140970 Msa0074000:intron 45.0%
ACTAGGTCCAGATCAGAAAG+AGG - chr1_2:48140961-48140980 None:intergenic 45.0%
! GATATATTTTCCGGTCAGGC+TGG - chr1_2:48141017-48141036 None:intergenic 45.0%
GTGAGATTGCCAGATATACC+TGG + chr1_2:48141390-48141409 Msa0074000:intron 45.0%
CCAGATATACCTGGTGAACT+CGG + chr1_2:48141399-48141418 Msa0074000:intron 45.0%
TGGATCCAACGAATCCTAGT+TGG + chr1_2:48141575-48141594 Msa0074000:CDS 45.0%
GGATCCAACGAATCCTAGTT+GGG + chr1_2:48141576-48141595 Msa0074000:CDS 45.0%
GATCCAACGAATCCTAGTTG+GGG + chr1_2:48141577-48141596 Msa0074000:CDS 45.0%
! AGTTAGGTGAACCCGGATTT+TGG - chr1_2:48141765-48141784 None:intergenic 45.0%
GATTGGAAGTTAGGTGAACC+CGG - chr1_2:48141772-48141791 None:intergenic 45.0%
CCTCACTATTGATCCAGCAA+TGG - chr1_2:48141926-48141945 None:intergenic 45.0%
CCATTGCTGGATCAATAGTG+AGG + chr1_2:48141923-48141942 Msa0074000:CDS 45.0%
ACACCAGAAAAAGGGTCCTT+TGG + chr1_2:48142054-48142073 Msa0074000:CDS 45.0%
! AGCTGCTTTCTACCTTTGGT+GGG - chr1_2:48142143-48142162 None:intergenic 45.0%
! AAGCTGCTTTCTACCTTTGG+TGG - chr1_2:48142144-48142163 None:intergenic 45.0%
TGATAACAGTCCCAAATGGG+GGG - chr1_2:48142478-48142497 None:intergenic 45.0%
TATTTGTGTTGCGTGTGGCT+TGG + chr1_2:48142624-48142643 Msa0074000:three_prime_UTR 45.0%
!! TGAATAAATAAAATAAAAAA+TGG - chr1_2:48142297-48142316 None:intergenic 5.0%
!!! TGTATATTTAATTTAATTAA+TGG + chr1_2:48142528-48142547 Msa0074000:three_prime_UTR 5.0%
!! GGAGGTGCTTGATCAGTTTC+TGG - chr1_2:48139013-48139032 None:intergenic 50.0%
! ACCTCCTTTTCATCCACCTG+AGG + chr1_2:48139027-48139046 Msa0074000:CDS 50.0%
TCAGCCTCAGGTGGATGAAA+AGG - chr1_2:48139034-48139053 None:intergenic 50.0%
! TTTTCATCCACCTGAGGCTG+AGG + chr1_2:48139033-48139052 Msa0074000:CDS 50.0%
ATAATCTCCTCCTCAGCCTC+AGG - chr1_2:48139046-48139065 None:intergenic 50.0%
TCAAGCCGCTCCAATTGTTC+AGG + chr1_2:48139171-48139190 Msa0074000:CDS 50.0%
GGCAACCTGAACAATTGGAG+CGG - chr1_2:48139179-48139198 None:intergenic 50.0%
!! GAGCATTTCGGGCTTTAAGG+GGG + chr1_2:48139536-48139555 Msa0074000:CDS 50.0%
TAAAGTCTGCATGGAGCGGA+GGG - chr1_2:48139620-48139639 None:intergenic 50.0%
GACCTAAAGTCTGCATGGAG+CGG - chr1_2:48139624-48139643 None:intergenic 50.0%
TGCAATCCCAGATTCGTTCG+AGG + chr1_2:48139647-48139666 Msa0074000:CDS 50.0%
AATCCCAGATTCGTTCGAGG+CGG + chr1_2:48139650-48139669 Msa0074000:CDS 50.0%
CACTTAGGATTCATGCCGTG+TGG + chr1_2:48140588-48140607 Msa0074000:intron 50.0%
GGAAAAGGAATCACCAGGCA+GGG - chr1_2:48140856-48140875 None:intergenic 50.0%
CATGAGTATTCCAGCCTGAC+CGG + chr1_2:48141004-48141023 Msa0074000:intron 50.0%
CCGAGTTCACCAGGTATATC+TGG - chr1_2:48141402-48141421 None:intergenic 50.0%
GGTAAATCACCGAGTTCACC+AGG - chr1_2:48141411-48141430 None:intergenic 50.0%
CATCCCCAACTAGGATTCGT+TGG - chr1_2:48141583-48141602 None:intergenic 50.0%
CAACGAATCCTAGTTGGGGA+TGG + chr1_2:48141581-48141600 Msa0074000:CDS 50.0%
GATGGAGCTGGTTAGAACGT+TGG + chr1_2:48141599-48141618 Msa0074000:CDS 50.0%
GAGCTGGTTAGAACGTTGGA+TGG + chr1_2:48141603-48141622 Msa0074000:CDS 50.0%
! CTTCTCCATCAGGCTTTGGT+TGG - chr1_2:48141632-48141651 None:intergenic 50.0%
CAACCAAAGCCTGATGGAGA+AGG + chr1_2:48141630-48141649 Msa0074000:CDS 50.0%
TCTCCTTCTCCATCAGGCTT+TGG - chr1_2:48141636-48141655 None:intergenic 50.0%
!! TTTCAGCACTGGTAATGCCG+CGG - chr1_2:48141684-48141703 None:intergenic 50.0%
TCCAACAGCGAGCCAAAATC+CGG + chr1_2:48141750-48141769 Msa0074000:CDS 50.0%
!! TGGGAGGCTTTGAGTTGGAT+TGG - chr1_2:48141789-48141808 None:intergenic 50.0%
!! TTGACAATTGCTGGCTTGGG+AGG - chr1_2:48141805-48141824 None:intergenic 50.0%
! CTGCACACTCGTCATGCTTT+TGG - chr1_2:48141878-48141897 None:intergenic 50.0%
AAGCATGACGAGTGTGCAGT+CGG + chr1_2:48141879-48141898 Msa0074000:CDS 50.0%
AGTCTGCAAAAGCCAAGTCC+AGG + chr1_2:48142001-48142020 Msa0074000:CDS 50.0%
TGGACTTTGCAACCTGGACT+TGG - chr1_2:48142016-48142035 None:intergenic 50.0%
!! AGCTGATGGACTTTGCAACC+TGG - chr1_2:48142022-48142041 None:intergenic 50.0%
GTAGTGTTCTCTGCAGCTGA+TGG - chr1_2:48142036-48142055 None:intergenic 50.0%
! TGAATGCCGCCTTGATGATC+TGG - chr1_2:48142118-48142137 None:intergenic 50.0%
AGATCATCAAGGCGGCATTC+AGG + chr1_2:48142117-48142136 Msa0074000:CDS 50.0%
GCCTCAGGTGGATGAAAAGG+AGG - chr1_2:48139031-48139050 None:intergenic 55.0%
AGGGCCAGTCGTAAAACGTC+AGG + chr1_2:48139585-48139604 Msa0074000:CDS 55.0%
! ACGGCCTGACGTTTTACGAC+TGG - chr1_2:48139592-48139611 None:intergenic 55.0%
!! CATGGAGCGGAGGGTACTAA+CGG - chr1_2:48139611-48139630 None:intergenic 55.0%
CTAAAGTCTGCATGGAGCGG+AGG - chr1_2:48139621-48139640 None:intergenic 55.0%
CTCCGCTCCATGCAGACTTT+AGG + chr1_2:48139619-48139638 Msa0074000:CDS 55.0%
ATCCCAGATTCGTTCGAGGC+GGG + chr1_2:48139651-48139670 Msa0074000:CDS 55.0%
GGGAAAAGGAATCACCAGGC+AGG - chr1_2:48140857-48140876 None:intergenic 55.0%
ATCCTAGTTGGGGATGGAGC+TGG + chr1_2:48141587-48141606 Msa0074000:CDS 55.0%
AACCAGCTCCATCCCCAACT+AGG - chr1_2:48141592-48141611 None:intergenic 55.0%
CCAACAGCGAGCCAAAATCC+GGG + chr1_2:48141751-48141770 Msa0074000:CDS 55.0%
!! TGGCTTGGGAGGCTTTGAGT+TGG - chr1_2:48141794-48141813 None:intergenic 55.0%
CTTCGCCTGCCAGATCATCA+AGG + chr1_2:48142106-48142125 Msa0074000:CDS 55.0%
AAATGGGGGGAATGCTCTCC+AGG - chr1_2:48142465-48142484 None:intergenic 55.0%
!! TGGAGAGCATTCCCCCCATT+TGG + chr1_2:48142464-48142483 Msa0074000:three_prime_UTR 55.0%
!! GGAGAGCATTCCCCCCATTT+GGG + chr1_2:48142465-48142484 Msa0074000:three_prime_UTR 55.0%
TCATCCACCTGAGGCTGAGG+AGG + chr1_2:48139036-48139055 Msa0074000:CDS 60.0%
ATCTCCTCCTCAGCCTCAGG+TGG - chr1_2:48139043-48139062 None:intergenic 60.0%
!! GGGCTTTAAGGGGGCTGAAC+AGG + chr1_2:48139545-48139564 Msa0074000:CDS 60.0%
GACCCGCCTCGAACGAATCT+GGG - chr1_2:48139656-48139675 None:intergenic 60.0%
TGACCCGCCTCGAACGAATC+TGG - chr1_2:48139657-48139676 None:intergenic 60.0%
AGATTCGTTCGAGGCGGGTC+AGG + chr1_2:48139656-48139675 Msa0074000:CDS 60.0%
GAGGCGGGTCAGGATGTTAG+AGG + chr1_2:48139666-48139685 Msa0074000:CDS 60.0%
GGCAGCCAACCAAAGCCTGA+TGG + chr1_2:48141624-48141643 Msa0074000:CDS 60.0%
!!! CCCGGATTTTGGCTCGCTGT+TGG - chr1_2:48141754-48141773 None:intergenic 60.0%
GTGTGCAGTCGGAGCGTGTT+CGG + chr1_2:48141890-48141909 Msa0074000:CDS 60.0%
ATGATCTGGCAGGCGAAGCC+GGG - chr1_2:48142104-48142123 None:intergenic 60.0%
GATGATCTGGCAGGCGAAGC+CGG - chr1_2:48142105-48142124 None:intergenic 60.0%
CGCCTGCCAGATCATCAAGG+CGG + chr1_2:48142109-48142128 Msa0074000:CDS 60.0%
! TGCCGCCTTGATGATCTGGC+AGG - chr1_2:48142114-48142133 None:intergenic 60.0%
TGCAGTCGGAGCGTGTTCGG+AGG + chr1_2:48141893-48141912 Msa0074000:CDS 65.0%
AGTCGGAGCGTGTTCGGAGG+AGG + chr1_2:48141896-48141915 Msa0074000:CDS 65.0%
!! CGGAGGAGGCACTCCATTGC+TGG + chr1_2:48141910-48141929 Msa0074000:CDS 65.0%
GCGGCATTCAGGCCCACCAA+AGG + chr1_2:48142128-48142147 Msa0074000:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 48138403 48142699 48138403 ID=Msa0074000;Name=Msa0074000
chr1_2 mRNA 48138403 48142699 48138403 ID=Msa0074000-mRNA-1;Parent=Msa0074000;Name=Msa0074000-mRNA-1;_AED=0.02;_eAED=0.02;_QI=231|1|1|1|1|1|5|496|466
chr1_2 exon 48138403 48138711 48138403 ID=Msa0074000-mRNA-1:exon:14624;Parent=Msa0074000-mRNA-1
chr1_2 exon 48138947 48139258 48138947 ID=Msa0074000-mRNA-1:exon:14625;Parent=Msa0074000-mRNA-1
chr1_2 exon 48139529 48139747 48139529 ID=Msa0074000-mRNA-1:exon:14626;Parent=Msa0074000-mRNA-1
chr1_2 exon 48139878 48139997 48139878 ID=Msa0074000-mRNA-1:exon:14627;Parent=Msa0074000-mRNA-1
chr1_2 exon 48141532 48142699 48141532 ID=Msa0074000-mRNA-1:exon:14628;Parent=Msa0074000-mRNA-1
chr1_2 five_prime_UTR 48138403 48138633 48138403 ID=Msa0074000-mRNA-1:five_prime_utr;Parent=Msa0074000-mRNA-1
chr1_2 CDS 48138634 48138711 48138634 ID=Msa0074000-mRNA-1:cds;Parent=Msa0074000-mRNA-1
chr1_2 CDS 48138947 48139258 48138947 ID=Msa0074000-mRNA-1:cds;Parent=Msa0074000-mRNA-1
chr1_2 CDS 48139529 48139747 48139529 ID=Msa0074000-mRNA-1:cds;Parent=Msa0074000-mRNA-1
chr1_2 CDS 48139878 48139997 48139878 ID=Msa0074000-mRNA-1:cds;Parent=Msa0074000-mRNA-1
chr1_2 CDS 48141532 48142203 48141532 ID=Msa0074000-mRNA-1:cds;Parent=Msa0074000-mRNA-1
chr1_2 three_prime_UTR 48142204 48142699 48142204 ID=Msa0074000-mRNA-1:three_prime_utr;Parent=Msa0074000-mRNA-1
Gene Sequence

>Msa0074000

ATGGGGAAGAATAAGCCAAATTGGTTTTCTAATGTGAAGAAAGCTCTTAGTCCTGACTCTAAACAAAACAAACATCAGAATTCAAGTGGATCAAAGAAGAAATGGTTTGGGAAGAAAAAATTAAACGATTCAGATTCATATCCAGAAACTGATCAAGCACCTCCTTTTCATCCACCTGAGGCTGAGGAGGAGATTATTTTAACTCATGTTGAGAATCAAAACAATCATGATCATGTTGAAGTTGTACCAGATGTGGATGTTGATGTACCTGTTTCTGATGTTCAAACAGAAACTGTTGAGGTTCAAGCCGCTCCAATTGTTCAGGTTGCCAGTAAACCGAATGATGAGGTTGCGGCAATCATGATTCAAACCACTTTTCGGCGATACTTGGCAAGAAGAGCATTTCGGGCTTTAAGGGGGCTGAACAGGTTGAGAGCACTAATGGAAGGGCCAGTCGTAAAACGTCAGGCCGTTAGTACCCTCCGCTCCATGCAGACTTTAGGTCATGTGCAATCCCAGATTCGTTCGAGGCGGGTCAGGATGTTAGAGGAGACTCAAGCTCTGCAAAAACAGCTCTTACAGAAGCACACAAAAGAGCTAGAGATACAGATTGGGGAGGAATGGGATGAAAGCATACAATCAAGAGAGCAAATTGAAGCCAAGTTACTAAGCAAGTACGAGGCTACTATGAGAAGAGAAAGAGCAATGGCTTATTCATTCACTCATCAGAAAAACGGGCGGAATTCATCGAAATCTATAAATCCAATGTTTATGGATCCAACGAATCCTAGTTGGGGATGGAGCTGGTTAGAACGTTGGATGGCAGCCAACCAAAGCCTGATGGAGAAGGAGAACAATTCATCTGTAAAAAGTTCGATCCGCGGCATTACCAGTGCTGAAATCAGCAAATCATTTGCTCGTTTCCAGCTCAATTCCGAAAATCATTCTCCAACAGCGAGCCAAAATCCGGGTTCACCTAACTTCCAATCCAACTCAAAGCCTCCCAAGCCAGCAATTGTCAAGAAACTGAATAAGGCTAGTCCAAAGGTGAGCTGTATTATGGATGATGACACCAAAAGCATGACGAGTGTGCAGTCGGAGCGTGTTCGGAGGAGGCACTCCATTGCTGGATCAATAGTGAGGGATGATGAAAGTCTTGCAAGCTCTCCATCAGTTCCAAGTTACATGGTGCCAACTAAGTCTGCAAAAGCCAAGTCCAGGTTGCAAAGTCCATCAGCTGCAGAGAACACTACACCAGAAAAAGGGTCCTTTGGAAATGCAAAGAAACGGCTTAACTTCCCGGCTTCGCCTGCCAGATCATCAAGGCGGCATTCAGGCCCACCAAAGGTAGAAAGCAGCTTAAATGCAGAAATTACTGTTGGCAATGGTGTGGCTGTTTGA
Protein sequence

>Msa0074000

MGKNKPNWFSNVKKALSPDSKQNKHQNSSGSKKKWFGKKKLNDSDSYPETDQAPPFHPPEAEEEIILTHVENQNNHDHVEVVPDVDVDVPVSDVQTETVEVQAAPIVQVASKPNDEVAAIMIQTTFRRYLARRAFRALRGLNRLRALMEGPVVKRQAVSTLRSMQTLGHVQSQIRSRRVRMLEETQALQKQLLQKHTKELEIQIGEEWDESIQSREQIEAKLLSKYEATMRRERAMAYSFTHQKNGRNSSKSINPMFMDPTNPSWGWSWLERWMAANQSLMEKENNSSVKSSIRGITSAEISKSFARFQLNSENHSPTASQNPGSPNFQSNSKPPKPAIVKKLNKASPKVSCIMDDDTKSMTSVQSERVRRRHSIAGSIVRDDESLASSPSVPSYMVPTKSAKAKSRLQSPSAAENTTPEKGSFGNAKKRLNFPASPARSSRRHSGPPKVESSLNAEITVGNGVAV*