AlfalfaGEDB | Msa0186800

M. sativa cultivar zhongmu-4 / Msa0186800

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186800	XP_003603145.1	88.261	230	11	1	1	214	1	230	5.52e-142	406

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186800	sp\|Q7XC57\|MYBS3_ORYSJ	36.923	195	90	4	3	176	1	183	3.08e-27	108

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186800	G7J7M5	88.261	230	11	1	1	214	1	230	2.64e-142	406

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TFs/TRs:

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Gene ID	Type	Classification
Msa0186800	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0140490	Msa0186800	0.975279	1.156487e-139	-8.615850e-47
Msa0186800	Msa0381760	0.814480	1.507754e-51	-8.615850e-47
Msa0186800	Msa1383440	0.964789	9.021466e-124	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186800	MtrunA17_Chr3g0135671	88.261	230	11	1	1	214	1	230	5.08e-146	406
Msa0186800	MtrunA17_Chr6g0488471	42.857	182	72	7	3	157	1	177	7.94e-33	120
Msa0186800	MtrunA17_Chr4g0055931	37.705	183	88	3	3	162	1	180	4.18e-30	113
Msa0186800	MtrunA17_Chr5g0415191	43.421	152	53	5	1	122	1	149	9.72e-28	107
Msa0186800	MtrunA17_Chr8g0389241	41.040	173	68	4	5	146	4	173	5.94e-27	104
Msa0186800	MtrunA17_Chr2g0331031	62.857	70	23	1	70	139	104	170	2.51e-23	95.9
Msa0186800	MtrunA17_Chr1g0210471	62.162	74	26	1	61	134	110	181	4.40e-23	93.2
Msa0186800	MtrunA17_Chr5g0429521	48.039	102	46	2	70	171	127	221	9.34e-23	93.6
Msa0186800	MtrunA17_Chr5g0436501	47.959	98	41	2	35	128	87	178	3.86e-22	91.3
Msa0186800	MtrunA17_Chr3g0104071	54.217	83	31	2	70	152	142	217	2.11e-21	90.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186800	AT5G56840.1	39.013	223	114	5	3	207	1	219	1.22e-32	118
Msa0186800	AT5G61620.1	39.884	173	73	5	1	145	6	175	3.68e-28	108
Msa0186800	AT5G47390.1	33.745	243	99	7	3	215	1	211	1.55e-27	107
Msa0186800	AT1G70000.1	39.310	145	63	3	3	122	1	145	1.50e-24	97.8
Msa0186800	AT1G70000.2	39.310	145	63	3	3	122	1	145	1.50e-24	97.8
Msa0186800	AT2G38090.1	62.500	64	24	0	70	133	136	199	1.21e-21	90.9
Msa0186800	AT5G58900.1	54.545	77	35	0	70	146	136	212	1.41e-21	90.5
Msa0186800	AT5G01200.1	60.938	64	25	0	70	133	141	204	2.53e-21	89.4
Msa0186800	AT5G04760.1	48.421	95	43	2	61	151	85	177	7.54e-21	87.0
Msa0186800	AT1G49010.1	55.263	76	31	2	67	142	126	198	8.65e-21	89.0

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Find 48 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGGTAGGCAAGGAGG+AGG	0.626173	2_1:-1564138	None:intergenic
AAATATCATGTGCTCCTCCT+CGG	0.585344	2_1:-1564552	None:intergenic
AAATCAGGTGCTGCACTATG+AGG	0.523156	2_1:-1565003	None:intergenic
AAGAGGTCCGCGATGATGGC+AGG	0.511716	2_1:-1564106	None:intergenic
AATTTCTACTTCCACTTAGT+TGG	0.375503	2_1:-1564903	None:intergenic
ACACTACTGGTTAGCTTAAG+GGG	0.538405	2_1:-1565076	None:intergenic
ACCAAACATGGTAGGCAAGG+AGG	0.664492	2_1:-1564141	None:intergenic
AGAACTACCTGCCATCATCG+CGG	0.745192	2_1:+1564099	Msa0186800:CDS
AGAAGCTTGGGAAGGGTAAC+TGG	0.328316	2_1:+1564588	Msa0186800:CDS
AGCTGCATAAGCCAACTAAG+TGG	0.584599	2_1:+1564892	Msa0186800:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGATATATGCTCGTTTCTTA+CGG	-	chr2_1:1564189-1564208	None:intergenic	30.0%
TAGAATTGTGAATTGTTGCT+TGG	+	chr2_1:1564479-1564498	Msa0186800:intron	30.0%
AATTTCTACTTCCACTTAGT+TGG	-	chr2_1:1564906-1564925	None:intergenic	30.0%
CTCAACATTCAATACTGAAA+TGG	+	chr2_1:1564950-1564969	Msa0186800:CDS	30.0%
TGATATATGCTCGTTTCTTA+CGG	-	chr2_1:1564189-1564208	None:intergenic	30.0%
TAGAATTGTGAATTGTTGCT+TGG	+	chr2_1:1564479-1564498	Msa0186800:intron	30.0%
AATTTCTACTTCCACTTAGT+TGG	-	chr2_1:1564906-1564925	None:intergenic	30.0%
CTCAACATTCAATACTGAAA+TGG	+	chr2_1:1564950-1564969	Msa0186800:CDS	30.0%
TGCTCATATTGTGGAAACTT+TGG	+	chr2_1:1563981-1564000	Msa0186800:CDS	35.0%
GAAACTTTGGTCACAATTCA+AGG	+	chr2_1:1563994-1564013	Msa0186800:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	1563963	1565083	1563963	ID=Msa0186800;Name=Msa0186800
chr2_1	mRNA	1563963	1565083	1563963	ID=Msa0186800-mRNA-1;Parent=Msa0186800;Name=Msa0186800-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|0.5\|0.33\|3\|0\|219
chr2_1	exon	1563963	1564182	1563963	ID=Msa0186800-mRNA-1:exon:618;Parent=Msa0186800-mRNA-1
chr2_1	exon	1564540	1564742	1564540	ID=Msa0186800-mRNA-1:exon:619;Parent=Msa0186800-mRNA-1
chr2_1	exon	1564847	1565083	1564847	ID=Msa0186800-mRNA-1:exon:620;Parent=Msa0186800-mRNA-1
chr2_1	CDS	1563963	1564182	1563963	ID=Msa0186800-mRNA-1:cds;Parent=Msa0186800-mRNA-1
chr2_1	CDS	1564540	1564742	1564540	ID=Msa0186800-mRNA-1:cds;Parent=Msa0186800-mRNA-1
chr2_1	CDS	1564847	1565083	1564847	ID=Msa0186800-mRNA-1:cds;Parent=Msa0186800-mRNA-1

Gene Sequence

>Msa0186800

ATGAGAATAGCTAGGAAATGCTCATATTGTGGAAACTTTGGTCACAATTCAAGGACTTGCAATAACAGTCTCAAAGAGCAATTTCATCTTTTTTCTTCTTCACCTTCATATTTGCCCACAAAAAGGAGTTTCAGAAAGAACTACCTGCCATCATCGCGGACCTCTTTATCAATAGCCTCCTCCTTGCCTACCATGTTTGGTTCAAATGAAAATTCAGATAGTATGCCGTGGACCGAGGAGGAGCACATGATATTTCTTAGAGGACTTGAGAAGCTTGGGAAGGGTAACTGGAGAGGTATATCTAGAGATTTTGTAACAACAAAAACACCAACCCAGGTAGCTAGTCATGCACAAAAACATTTTCTCCGCCAGTCTCAGAATAGCTTGATCAATAGAAGAAAACACCATCTTAGCCTCCATTATGTGGGATGTGAAAAACCAGGAATAAATTTGACTCCAAATGATATCAGCTGCATAAGCCAACTAAGTGGAAGTAGAAATTTTCTTCCTCAGTGGCTTTCTCATCCTCAACATTCAATACTGAAATGGCCTAACTCTTCAACATCAACGGATTGTACTCCTCATAGTGCAGCACCTGATTTAGAACTCAAACTTGCAACTCCCATGCCATTAGAGCTAACAGAGGCATGCTCCCCTTAA

Protein sequence

>Msa0186800

MRIARKCSYCGNFGHNSRTCNNSLKEQFHLFSSSPSYLPTKRSFRKNYLPSSRTSLSIASSLPTMFGSNENSDSMPWTEEEHMIFLRGLEKLGKGNWRGISRDFVTTKTPTQVASHAQKHFLRQSQNSLINRRKHHLSLHYVGCEKPGINLTPNDISCISQLSGSRNFLPQWLSHPQHSILKWPNSSTSTDCTPHSAAPDLELKLATPMPLELTEACSP*