Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187260 | P48488.1 | 100.000 | 321 | 0 | 0 | 1 | 321 | 1 | 321 | 0.0 | 665 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187260 | sp|P48488|PP1_MEDSV | 100.000 | 321 | 0 | 0 | 1 | 321 | 1 | 321 | 0.0 | 665 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187260 | B7FHM9 | 99.688 | 321 | 1 | 0 | 1 | 321 | 1 | 321 | 0.0 | 662 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0187260 | Msa0526330 | 0.994664 | 3.884360e-209 | -8.615850e-47 |
| Msa0187260 | Msa0572000 | 0.819751 | 9.851479e-53 | -8.615850e-47 |
| Msa0187260 | Msa0699190 | 0.804812 | 1.804913e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187260 | MtrunA17_Chr8g0387541 | 99.688 | 321 | 1 | 0 | 1 | 321 | 1 | 321 | 0.0 | 662 |
| Msa0187260 | MtrunA17_Chr1g0190121 | 79.114 | 316 | 64 | 2 | 1 | 315 | 1 | 315 | 0.0 | 540 |
| Msa0187260 | MtrunA17_Chr7g0274931 | 78.165 | 316 | 67 | 2 | 1 | 315 | 1 | 315 | 0.0 | 533 |
| Msa0187260 | MtrunA17_Chr5g0436061 | 78.431 | 306 | 66 | 0 | 1 | 306 | 17 | 322 | 0.0 | 523 |
| Msa0187260 | MtrunA17_Chr1g0186811 | 74.603 | 315 | 78 | 2 | 1 | 314 | 1 | 314 | 0.0 | 511 |
| Msa0187260 | MtrunA17_Chr1g0167751 | 75.161 | 310 | 75 | 2 | 1 | 309 | 1 | 309 | 2.20e-180 | 501 |
| Msa0187260 | MtrunA17_Chr7g0272371 | 72.500 | 320 | 79 | 3 | 1 | 318 | 5 | 317 | 9.03e-177 | 491 |
| Msa0187260 | MtrunA17_Chr8g0334361 | 77.163 | 289 | 65 | 1 | 6 | 294 | 4 | 291 | 5.01e-174 | 484 |
| Msa0187260 | MtrunA17_Chr8g0378521 | 46.128 | 297 | 150 | 3 | 6 | 301 | 9 | 296 | 2.31e-96 | 287 |
| Msa0187260 | MtrunA17_Chr7g0268381 | 47.826 | 276 | 142 | 2 | 27 | 301 | 27 | 301 | 1.55e-95 | 285 |
| Msa0187260 | MtrunA17_Chr5g0415261 | 48.679 | 265 | 134 | 2 | 27 | 290 | 20 | 283 | 1.23e-93 | 280 |
| Msa0187260 | MtrunA17_Chr8g0364041 | 48.302 | 265 | 135 | 2 | 27 | 290 | 20 | 283 | 1.43e-93 | 280 |
| Msa0187260 | MtrunA17_Chr5g0445011 | 44.948 | 287 | 154 | 2 | 27 | 312 | 17 | 300 | 4.54e-93 | 279 |
| Msa0187260 | MtrunA17_Chr1g0165341 | 41.158 | 311 | 168 | 6 | 6 | 314 | 3 | 300 | 4.63e-90 | 271 |
| Msa0187260 | MtrunA17_Chr2g0322121 | 50.000 | 272 | 122 | 5 | 23 | 280 | 660 | 931 | 7.55e-82 | 267 |
| Msa0187260 | MtrunA17_Chr4g0025471 | 45.603 | 307 | 151 | 6 | 3 | 293 | 643 | 949 | 3.36e-80 | 262 |
| Msa0187260 | MtrunA17_Chr3g0097231 | 42.160 | 287 | 155 | 5 | 3 | 283 | 168 | 449 | 2.63e-70 | 226 |
| Msa0187260 | MtrunA17_Chr2g0330311 | 41.404 | 285 | 154 | 7 | 3 | 280 | 195 | 473 | 1.09e-65 | 214 |
| Msa0187260 | MtrunA17_Chr2g0330331 | 40.484 | 289 | 160 | 7 | 2 | 283 | 32 | 315 | 3.41e-61 | 198 |
| Msa0187260 | MtrunA17_Chr1g0196211 | 47.222 | 180 | 85 | 5 | 109 | 279 | 2 | 180 | 9.69e-49 | 163 |
| Msa0187260 | MtrunA17_Chr8g0369981 | 31.111 | 315 | 152 | 10 | 34 | 283 | 100 | 414 | 6.37e-36 | 134 |
| Msa0187260 | MtrunA17_Chr1g0196221 | 46.296 | 108 | 50 | 2 | 9 | 108 | 528 | 635 | 3.44e-24 | 103 |
| Msa0187260 | MtrunA17_Chr2g0330011 | 36.975 | 119 | 65 | 4 | 32 | 141 | 232 | 349 | 3.67e-16 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187260 | AT1G64040.1 | 85.266 | 319 | 47 | 0 | 1 | 319 | 1 | 319 | 0.0 | 589 |
| Msa0187260 | AT4G11240.1 | 83.438 | 320 | 53 | 0 | 1 | 320 | 1 | 320 | 0.0 | 585 |
| Msa0187260 | AT2G29400.1 | 81.457 | 302 | 53 | 2 | 1 | 300 | 15 | 315 | 0.0 | 526 |
| Msa0187260 | AT2G39840.1 | 79.070 | 301 | 63 | 0 | 1 | 301 | 14 | 314 | 0.0 | 516 |
| Msa0187260 | AT5G59160.3 | 78.146 | 302 | 65 | 1 | 1 | 301 | 9 | 310 | 0.0 | 509 |
| Msa0187260 | AT5G59160.2 | 78.146 | 302 | 65 | 1 | 1 | 301 | 9 | 310 | 0.0 | 509 |
| Msa0187260 | AT5G59160.1 | 78.146 | 302 | 65 | 1 | 1 | 301 | 9 | 310 | 0.0 | 509 |
| Msa0187260 | AT3G46820.1 | 77.152 | 302 | 68 | 1 | 1 | 301 | 9 | 310 | 0.0 | 503 |
| Msa0187260 | AT5G43380.1 | 70.717 | 321 | 93 | 1 | 1 | 321 | 1 | 320 | 1.03e-175 | 489 |
| Msa0187260 | AT5G43380.2 | 71.519 | 316 | 89 | 1 | 1 | 316 | 1 | 315 | 4.06e-175 | 488 |
| Msa0187260 | AT5G43380.3 | 70.717 | 321 | 93 | 1 | 1 | 321 | 1 | 320 | 5.50e-175 | 488 |
| Msa0187260 | AT5G27840.1 | 75.338 | 296 | 70 | 1 | 1 | 296 | 9 | 301 | 6.51e-174 | 484 |
| Msa0187260 | AT5G27840.4 | 73.786 | 309 | 73 | 2 | 1 | 304 | 9 | 314 | 7.38e-174 | 485 |
| Msa0187260 | AT5G27840.2 | 75.338 | 296 | 70 | 1 | 1 | 296 | 9 | 301 | 1.43e-173 | 484 |
| Msa0187260 | AT5G27840.3 | 75.338 | 296 | 70 | 1 | 1 | 296 | 9 | 301 | 1.43e-173 | 484 |
| Msa0187260 | AT3G05580.1 | 76.014 | 296 | 68 | 1 | 1 | 296 | 9 | 301 | 3.67e-173 | 483 |
| Msa0187260 | AT3G05580.2 | 75.304 | 247 | 58 | 1 | 1 | 247 | 9 | 252 | 8.32e-141 | 398 |
| Msa0187260 | AT2G42500.1 | 47.138 | 297 | 147 | 3 | 6 | 301 | 14 | 301 | 5.31e-98 | 292 |
| Msa0187260 | AT3G58500.1 | 46.465 | 297 | 149 | 3 | 6 | 301 | 14 | 301 | 1.04e-96 | 288 |
| Msa0187260 | AT5G55260.1 | 46.739 | 276 | 146 | 1 | 27 | 301 | 17 | 292 | 7.56e-95 | 283 |
| Msa0187260 | AT1G10430.2 | 46.503 | 286 | 143 | 3 | 6 | 290 | 7 | 283 | 1.77e-94 | 283 |
| Msa0187260 | AT1G10430.1 | 46.503 | 286 | 143 | 3 | 6 | 290 | 7 | 283 | 1.77e-94 | 283 |
| Msa0187260 | AT1G59830.1 | 46.853 | 286 | 142 | 3 | 6 | 290 | 7 | 283 | 4.55e-94 | 281 |
| Msa0187260 | AT1G69960.1 | 47.547 | 265 | 137 | 2 | 27 | 290 | 21 | 284 | 4.28e-93 | 279 |
| Msa0187260 | AT4G26720.1 | 43.310 | 284 | 160 | 1 | 19 | 301 | 9 | 292 | 2.10e-92 | 277 |
| Msa0187260 | AT1G50370.1 | 40.453 | 309 | 172 | 5 | 8 | 314 | 2 | 300 | 3.59e-90 | 271 |
| Msa0187260 | AT3G19980.1 | 40.453 | 309 | 172 | 5 | 8 | 314 | 2 | 300 | 5.68e-90 | 271 |
| Msa0187260 | AT5G55260.2 | 40.694 | 317 | 146 | 2 | 27 | 301 | 17 | 333 | 1.73e-86 | 264 |
| Msa0187260 | AT1G59830.2 | 48.400 | 250 | 119 | 3 | 6 | 254 | 7 | 247 | 1.95e-83 | 253 |
| Msa0187260 | AT2G27210.2 | 50.000 | 272 | 122 | 5 | 23 | 280 | 666 | 937 | 1.82e-82 | 269 |
| Msa0187260 | AT2G27210.1 | 50.000 | 272 | 122 | 5 | 23 | 280 | 671 | 942 | 1.93e-82 | 269 |
| Msa0187260 | AT1G08420.2 | 49.632 | 272 | 123 | 5 | 23 | 280 | 677 | 948 | 1.39e-81 | 266 |
| Msa0187260 | AT1G08420.1 | 49.632 | 272 | 123 | 5 | 23 | 280 | 682 | 953 | 1.47e-81 | 266 |
| Msa0187260 | AT4G03080.1 | 45.364 | 302 | 147 | 7 | 9 | 293 | 530 | 830 | 1.19e-80 | 262 |
| Msa0187260 | AT2G42500.2 | 49.327 | 223 | 111 | 2 | 80 | 301 | 33 | 254 | 1.60e-76 | 235 |
| Msa0187260 | AT1G03445.1 | 42.857 | 301 | 149 | 8 | 3 | 282 | 446 | 744 | 8.69e-73 | 239 |
| Msa0187260 | AT1G03445.2 | 42.857 | 301 | 149 | 8 | 3 | 282 | 446 | 744 | 9.25e-73 | 239 |
| Msa0187260 | AT2G42810.1 | 40.702 | 285 | 157 | 5 | 2 | 280 | 170 | 448 | 9.04e-67 | 217 |
| Msa0187260 | AT2G42810.5 | 40.702 | 285 | 157 | 5 | 2 | 280 | 170 | 448 | 9.04e-67 | 217 |
| Msa0187260 | AT2G42810.4 | 40.702 | 285 | 157 | 5 | 2 | 280 | 170 | 448 | 9.04e-67 | 217 |
| Msa0187260 | AT2G42810.2 | 40.702 | 285 | 157 | 5 | 2 | 280 | 224 | 502 | 2.49e-66 | 217 |
| Msa0187260 | AT2G42810.3 | 40.702 | 285 | 157 | 5 | 2 | 280 | 223 | 501 | 2.62e-66 | 217 |
| Msa0187260 | AT2G42500.4 | 45.238 | 168 | 80 | 3 | 6 | 169 | 14 | 173 | 1.86e-44 | 150 |
| Msa0187260 | AT2G42500.5 | 44.571 | 175 | 83 | 4 | 6 | 176 | 14 | 178 | 2.63e-44 | 150 |
| Msa0187260 | AT2G42500.3 | 46.250 | 160 | 74 | 3 | 6 | 161 | 14 | 165 | 1.07e-43 | 148 |
| Msa0187260 | AT5G63870.1 | 30.303 | 264 | 134 | 7 | 56 | 269 | 79 | 342 | 5.20e-31 | 121 |
| Msa0187260 | AT5G63870.2 | 30.303 | 264 | 134 | 7 | 56 | 269 | 79 | 342 | 5.20e-31 | 121 |
| Msa0187260 | AT5G63870.3 | 30.000 | 220 | 119 | 5 | 56 | 240 | 79 | 298 | 2.30e-25 | 103 |
| Msa0187260 | AT1G48120.1 | 28.477 | 302 | 144 | 8 | 34 | 269 | 630 | 925 | 5.83e-25 | 106 |
| Msa0187260 | AT5G10900.2 | 29.885 | 261 | 143 | 10 | 56 | 278 | 225 | 483 | 1.14e-20 | 93.2 |
| Msa0187260 | AT5G10900.3 | 29.885 | 261 | 143 | 10 | 56 | 278 | 225 | 483 | 1.39e-20 | 92.8 |
| Msa0187260 | AT5G10900.1 | 29.885 | 261 | 143 | 10 | 56 | 278 | 225 | 483 | 1.46e-20 | 92.8 |
Find 114 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTCCATCAATCGTATTTA+TGG | 0.118761 | 2_1:-1957344 | Msa0187260:CDS |
| TCATTCTCCACATAGGTTAA+AGG | 0.225911 | 2_1:-1955045 | Msa0187260:three_prime_UTR |
| GAAGCAAATTACTTATTTCT+TGG | 0.231778 | 2_1:-1957476 | Msa0187260:CDS |
| TCAAACTCACCACAGTAATT+TGG | 0.239332 | 2_1:+1956313 | None:intergenic |
| ACCTTATGAGGTGGAGTTCC+AGG | 0.245870 | 2_1:+1956181 | None:intergenic |
| CGAGGTGTTTCATTTACATT+TGG | 0.254753 | 2_1:-1957065 | Msa0187260:CDS |
| TATCGGAAGACTTCAGTATC+TGG | 0.265509 | 2_1:+1956242 | None:intergenic |
| TCCAACTTTGCCCTTCTTAT+CGG | 0.312310 | 2_1:+1956225 | None:intergenic |
| CCATGATCTGGCACATCAAT+AGG | 0.319380 | 2_1:+1957152 | None:intergenic |
| AGAAATAAGTAATTTGCTTC+AGG | 0.320791 | 2_1:+1957479 | None:intergenic |
| CATAACAAGATTACCCCTTA+TGG | 0.333035 | 2_1:+1954807 | None:intergenic |
| GAGGTGTTTCATTTACATTT+GGG | 0.340319 | 2_1:-1957064 | Msa0187260:CDS |
| ATTGAAGATGTATCATCAAC+TGG | 0.347192 | 2_1:-1959052 | Msa0187260:five_prime_UTR |
| TGATTGCTTTAATTGTTTAC+CGG | 0.357293 | 2_1:-1957267 | Msa0187260:CDS |
| GCTTCAAGTTCAAGAAGATT+AGG | 0.359782 | 2_1:+1958725 | None:intergenic |
| AAGAAGGGCAAAGTTGGATT+TGG | 0.364950 | 2_1:-1956220 | Msa0187260:CDS |
| CGTATTCGAACAGCCTTAAA+AGG | 0.372745 | 2_1:+1957513 | None:intergenic |
| AGGAGAACTTCTTTCTTCTC+AGG | 0.373373 | 2_1:-1957382 | Msa0187260:CDS |
| TGGAAATTCAAACTCCATAA+GGG | 0.384009 | 2_1:-1954821 | Msa0187260:three_prime_UTR |
| CATCCCTCGAGATCTTTATC+AGG | 0.394159 | 2_1:+1957101 | None:intergenic |
| GCCTTCTCTGCGACCTTCTG+TGG | 0.394394 | 2_1:-1957130 | Msa0187260:CDS |
| GCCTTAAAAGGTCTGAAAAC+TGG | 0.396799 | 2_1:+1957525 | None:intergenic |
| ATGGAAATTCAAACTCCATA+AGG | 0.400234 | 2_1:-1954822 | Msa0187260:three_prime_UTR |
| GAAAGAAAGAAACAAGGATA+GGG | 0.426060 | 2_1:+1954890 | None:intergenic |
| CATAAGGGGTAATCTTGTTA+TGG | 0.434936 | 2_1:-1954806 | Msa0187260:three_prime_UTR |
| GATTGATGGAGGCGCATTCA+TGG | 0.439246 | 2_1:+1957354 | None:intergenic |
| GAAACCATAAATACGATTGA+TGG | 0.442568 | 2_1:+1957340 | None:intergenic |
| TACAGTCACCAGCTTACGTT+TGG | 0.445882 | 2_1:+1956345 | None:intergenic |
| GATGTATCATCAACTGGGCT+AGG | 0.451995 | 2_1:-1959046 | Msa0187260:five_prime_UTR |
| ACCTGGAACTCCACCTCATA+AGG | 0.452558 | 2_1:-1956182 | Msa0187260:intron |
| GAAGCTCCTATCAAAATTTG+TGG | 0.455039 | 2_1:-1958707 | Msa0187260:intron |
| TATATTTCTGCTACCTGGCA+TGG | 0.457357 | 2_1:-1955020 | Msa0187260:three_prime_UTR |
| CCTATTGATGTGCCAGATCA+TGG | 0.458791 | 2_1:-1957152 | Msa0187260:CDS |
| ACTCGAGCTATGTAACTATA+AGG | 0.460438 | 2_1:-1955090 | Msa0187260:three_prime_UTR |
| AAAAGGAATTAAATAAAGTA+AGG | 0.468373 | 2_1:-1959111 | None:intergenic |
| GGAGGTATAATGTTCGTCTC+TGG | 0.469205 | 2_1:-1957301 | Msa0187260:CDS |
| TTGTCGTTGCAAAGGAAGGC+AGG | 0.472638 | 2_1:+1954968 | None:intergenic |
| AGAAAGAAAGAAACAAGGAT+AGG | 0.472918 | 2_1:+1954889 | None:intergenic |
| CCCACAGAAGGTCGCAGAGA+AGG | 0.474160 | 2_1:+1957129 | None:intergenic |
| ACTGAAGTCTTCCGATAAGA+AGG | 0.476810 | 2_1:-1956236 | Msa0187260:CDS |
| AGAAGATCCTTTGCATGCAT+GGG | 0.477596 | 2_1:-1957226 | Msa0187260:CDS |
| TCGCAGAGAAGGCCATGATC+TGG | 0.483085 | 2_1:+1957140 | None:intergenic |
| CATGATCTGGCACATCAATA+GGG | 0.484326 | 2_1:+1957153 | None:intergenic |
| TCCGATAAGAAGGGCAAAGT+TGG | 0.484368 | 2_1:-1956226 | Msa0187260:CDS |
| ACACCGAGTGTGACAGCCTA+TGG | 0.484463 | 2_1:-1954746 | Msa0187260:three_prime_UTR |
| GAGAGAAACGCGAAACGGAA+AGG | 0.491445 | 2_1:+1958939 | None:intergenic |
| GGAGAACTTCTTTCTTCTCA+GGG | 0.491751 | 2_1:-1957381 | Msa0187260:CDS |
| AATAAGTAATTTGCTTCAGG+TGG | 0.494184 | 2_1:+1957482 | None:intergenic |
| GCGTCCCAGGTTGTAGAAGA+TGG | 0.498692 | 2_1:-1956382 | Msa0187260:intron |
| AAAGAGAGAGAAACGCGAAA+CGG | 0.499054 | 2_1:+1958934 | None:intergenic |
| GAGAAGATCCTTTGCATGCA+TGG | 0.499556 | 2_1:-1957227 | Msa0187260:CDS |
| TGATAAAGATCTCGAGGGAT+GGG | 0.500185 | 2_1:-1957099 | Msa0187260:CDS |
| TAACTATAAGGTCAGTGAGC+TGG | 0.504000 | 2_1:-1955078 | Msa0187260:three_prime_UTR |
| AGGTTTATATTTCTGCTACC+TGG | 0.505060 | 2_1:-1955025 | Msa0187260:three_prime_UTR |
| AACGACAACTGAAGACAGTA+TGG | 0.510813 | 2_1:-1954953 | Msa0187260:three_prime_UTR |
| TCAGCTTTGTACTTCTGCTA+AGG | 0.513143 | 2_1:-1958765 | Msa0187260:CDS |
| TCTCTTGGAGATGGATGATA+CGG | 0.513600 | 2_1:-1958873 | Msa0187260:exon |
| TTCTTATTATTGATGTACAA+TGG | 0.516811 | 2_1:-1954841 | Msa0187260:three_prime_UTR |
| ATGGCTTTCATTCTCCACAT+AGG | 0.527219 | 2_1:-1955052 | Msa0187260:three_prime_UTR |
| CCTTCTCTGCGACCTTCTGT+GGG | 0.527902 | 2_1:-1957129 | Msa0187260:CDS |
| GTCTTCAGTTGTCGTTGCAA+AGG | 0.528795 | 2_1:+1954960 | None:intergenic |
| CTGATAAAGATCTCGAGGGA+TGG | 0.534435 | 2_1:-1957100 | Msa0187260:CDS |
| AAGGTCAGTGAGCTGGATGA+TGG | 0.534613 | 2_1:-1955071 | Msa0187260:three_prime_UTR |
| TGTGAATCATCAACCATGCC+AGG | 0.545199 | 2_1:+1955007 | None:intergenic |
| TTTAAGGCTGTTCGAATACG+GGG | 0.546220 | 2_1:-1957510 | Msa0187260:CDS |
| ACAACTGAAGACAGTATGGC+AGG | 0.547593 | 2_1:-1954949 | Msa0187260:three_prime_UTR |
| ATATAAACCTTTAACCTATG+TGG | 0.548666 | 2_1:+1955038 | None:intergenic |
| TCAGTTGTCGTTGCAAAGGA+AGG | 0.548844 | 2_1:+1954964 | None:intergenic |
| ATTTACATTTGGGGCTGACA+AGG | 0.548915 | 2_1:-1957054 | Msa0187260:CDS |
| AAAGGTCTGAAAACTGGCCA+TGG | 0.549539 | 2_1:+1957531 | None:intergenic |
| TCTGATATGTAGAGCTCACC+AGG | 0.551295 | 2_1:-1957000 | Msa0187260:intron |
| CTCAGTCGTTTACAAGAAGG+AGG | 0.558503 | 2_1:-1959012 | Msa0187260:five_prime_UTR |
| AGGTGTTTCATTTACATTTG+GGG | 0.560742 | 2_1:-1957063 | Msa0187260:CDS |
| GTTTACAAGAAGGAGGGAAA+CGG | 0.565321 | 2_1:-1959005 | Msa0187260:five_prime_UTR |
| TTGTTTGCAGGAGATGTCCA+TGG | 0.568088 | 2_1:-1957548 | Msa0187260:intron |
| TCATCTACTAGAGCAGCAAC+CGG | 0.568940 | 2_1:+1957248 | None:intergenic |
| TCGCCATAGGCTGTCACACT+CGG | 0.569823 | 2_1:+1954743 | None:intergenic |
| TGCATATAAAATCAAATACA+AGG | 0.570871 | 2_1:-1957402 | Msa0187260:CDS |
| GATAAAGATCTCGAGGGATG+GGG | 0.570941 | 2_1:-1957098 | Msa0187260:CDS |
| ATGTATCATCAACTGGGCTA+GGG | 0.572489 | 2_1:-1959045 | Msa0187260:five_prime_UTR |
| AAACTCAGTCGTTTACAAGA+AGG | 0.574886 | 2_1:-1959015 | Msa0187260:five_prime_UTR |
| TGTGGTGAGTTTGATAATGC+CGG | 0.578788 | 2_1:-1956304 | Msa0187260:CDS |
| CTTGGAGATTATGTTGATCG+TGG | 0.584311 | 2_1:-1957458 | Msa0187260:CDS |
| AAAGAAAGAAACAAGGATAG+GGG | 0.585866 | 2_1:+1954891 | None:intergenic |
| ATCCACACTCATCATAGCAC+CGG | 0.591401 | 2_1:+1956285 | None:intergenic |
| GGCAATAACTCATCAAGACC+TGG | 0.595098 | 2_1:-1956199 | Msa0187260:CDS |
| TATCAGGATCAGCCCACAGA+AGG | 0.599205 | 2_1:+1957117 | None:intergenic |
| CTGAAGTCTTCCGATAAGAA+GGG | 0.604771 | 2_1:-1956235 | Msa0187260:CDS |
| TGATCCTGATAAAGATCTCG+AGG | 0.605585 | 2_1:-1957105 | Msa0187260:CDS |
| ATAAGTAATTTGCTTCAGGT+GGG | 0.616465 | 2_1:+1957483 | None:intergenic |
| AAGACAGTATGGCAGGGACA+TGG | 0.622806 | 2_1:-1954942 | Msa0187260:three_prime_UTR |
| GAAGATCCTTTGCATGCATG+GGG | 0.624730 | 2_1:-1957225 | Msa0187260:CDS |
| TGTGACAGCCTATGGCGAGT+AGG | 0.625556 | 2_1:-1954738 | Msa0187260:three_prime_UTR |
| TACAAGAAGGAGGGAAACGG+CGG | 0.626522 | 2_1:-1959002 | Msa0187260:five_prime_UTR |
| GGAAATTCAAACTCCATAAG+GGG | 0.629390 | 2_1:-1954820 | Msa0187260:three_prime_UTR |
| TTGAAGATGTATCATCAACT+GGG | 0.634964 | 2_1:-1959051 | Msa0187260:five_prime_UTR |
| AAGAAAGAAACAAGGATAGG+GGG | 0.636253 | 2_1:+1954892 | None:intergenic |
| AAGCTTGTATCAGCGAAGAA+CGG | 0.636419 | 2_1:-1958830 | Msa0187260:CDS |
| CAACTGAAGACAGTATGGCA+GGG | 0.642387 | 2_1:-1954948 | Msa0187260:three_prime_UTR |
| GTTTCTACGATGAGTGCAAG+AGG | 0.643121 | 2_1:-1957322 | Msa0187260:CDS |
| TGTCGTTGCAAAGGAAGGCA+GGG | 0.643824 | 2_1:+1954969 | None:intergenic |
| AAGCTGACGAATATCAGCCT+CGG | 0.647938 | 2_1:+1958781 | None:intergenic |
| ACCATAAATACGATTGATGG+AGG | 0.650130 | 2_1:+1957343 | None:intergenic |
| AAATAAGAAAGAAAGAAACA+AGG | 0.650216 | 2_1:+1954884 | None:intergenic |
| TCAGTCGTTTACAAGAAGGA+GGG | 0.661164 | 2_1:-1959011 | Msa0187260:five_prime_UTR |
| GAGAACTTCTTTCTTCTCAG+GGG | 0.674269 | 2_1:-1957380 | Msa0187260:CDS |
| TGCCGGTGCTATGATGAGTG+TGG | 0.686901 | 2_1:-1956287 | Msa0187260:CDS |
| TTAAGGCTGTTCGAATACGG+GGG | 0.692495 | 2_1:-1957509 | Msa0187260:CDS |
| TCTACGATGAGTGCAAGAGG+AGG | 0.698302 | 2_1:-1957319 | Msa0187260:CDS |
| GGATGGGGAGAAAATGATCG+AGG | 0.699695 | 2_1:-1957083 | Msa0187260:CDS |
| TTCTCTGCACCAAATTACTG+TGG | 0.705308 | 2_1:-1956322 | Msa0187260:CDS |
| GATCCTGATAAAGATCTCGA+GGG | 0.709098 | 2_1:-1957104 | Msa0187260:CDS |
| AAGATCCTTTGCATGCATGG+GGG | 0.732102 | 2_1:-1957224 | Msa0187260:CDS |
| GAAGCAAGTTCATCTTACCG+AGG | 0.743441 | 2_1:-1958798 | Msa0187260:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAGAAAGAAAGAAACA+AGG | + | chr2_1:1958944-1958963 | None:intergenic | 20.0% |
| !! | TTCTTATTATTGATGTACAA+TGG | - | chr2_1:1958984-1959003 | Msa0187260:five_prime_UTR | 20.0% |
| ATTGAAGATGTATCATCAAC+TGG | - | chr2_1:1954773-1954792 | Msa0187260:three_prime_UTR | 30.0% | |
| TTGAAGATGTATCATCAACT+GGG | - | chr2_1:1954774-1954793 | Msa0187260:three_prime_UTR | 30.0% | |
| AGAAAGAAAGAAACAAGGAT+AGG | + | chr2_1:1958939-1958958 | None:intergenic | 30.0% | |
| ATGGAAATTCAAACTCCATA+AGG | - | chr2_1:1959003-1959022 | Msa0187260:five_prime_UTR | 30.0% | |
| TGGAAATTCAAACTCCATAA+GGG | - | chr2_1:1959004-1959023 | Msa0187260:five_prime_UTR | 30.0% | |
| GGAAATTCAAACTCCATAAG+GGG | - | chr2_1:1959005-1959024 | Msa0187260:five_prime_UTR | 35.0% | |
| CATAACAAGATTACCCCTTA+TGG | + | chr2_1:1959021-1959040 | None:intergenic | 35.0% | |
| CATAAGGGGTAATCTTGTTA+TGG | - | chr2_1:1959019-1959038 | Msa0187260:five_prime_UTR | 35.0% | |
| ! | ATATTTTTCCTACTCGCCAT+AGG | + | chr2_1:1959098-1959117 | None:intergenic | 35.0% |
| ACACCGAGTGTGACAGCCTA+TGG | - | chr2_1:1959079-1959098 | Msa0187260:five_prime_UTR | 55.0% | |
| TCGCCATAGGCTGTCACACT+CGG | + | chr2_1:1959085-1959104 | None:intergenic | 55.0% | |
| TGTGACAGCCTATGGCGAGT+AGG | - | chr2_1:1959087-1959106 | Msa0187260:five_prime_UTR | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 1954725 | 1959122 | 1954725 | ID=Msa0187260;Name=Msa0187260 |
| chr2_1 | mRNA | 1954725 | 1959122 | 1954725 | ID=Msa0187260-mRNA-1;Parent=Msa0187260;Name=Msa0187260-mRNA-1;_AED=0.05;_eAED=0.05;_QI=237|1|1|1|0.66|0.5|4|395|321 |
| chr2_1 | exon | 1954725 | 1955134 | 1954725 | ID=Msa0187260-mRNA-1:exon:907;Parent=Msa0187260-mRNA-1 |
| chr2_1 | exon | 1956183 | 1956395 | 1956183 | ID=Msa0187260-mRNA-1:exon:906;Parent=Msa0187260-mRNA-1 |
| chr2_1 | exon | 1957001 | 1957560 | 1957001 | ID=Msa0187260-mRNA-1:exon:905;Parent=Msa0187260-mRNA-1 |
| chr2_1 | exon | 1958708 | 1959122 | 1958708 | ID=Msa0187260-mRNA-1:exon:904;Parent=Msa0187260-mRNA-1 |
| chr2_1 | five_prime_UTR | 1958886 | 1959122 | 1958886 | ID=Msa0187260-mRNA-1:five_prime_utr;Parent=Msa0187260-mRNA-1 |
| chr2_1 | CDS | 1958708 | 1958885 | 1958708 | ID=Msa0187260-mRNA-1:cds;Parent=Msa0187260-mRNA-1 |
| chr2_1 | CDS | 1957001 | 1957560 | 1957001 | ID=Msa0187260-mRNA-1:cds;Parent=Msa0187260-mRNA-1 |
| chr2_1 | CDS | 1956183 | 1956395 | 1956183 | ID=Msa0187260-mRNA-1:cds;Parent=Msa0187260-mRNA-1 |
| chr2_1 | CDS | 1955120 | 1955134 | 1955120 | ID=Msa0187260-mRNA-1:cds;Parent=Msa0187260-mRNA-1 |
| chr2_1 | three_prime_UTR | 1954725 | 1955119 | 1954725 | ID=Msa0187260-mRNA-1:three_prime_utr;Parent=Msa0187260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
