Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572000 | P48488.1 | 95.584 | 317 | 0 | 2 | 1 | 303 | 1 | 317 | 0.0 | 614 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572000 | sp|P48488|PP1_MEDSV | 95.584 | 317 | 0 | 2 | 1 | 303 | 1 | 317 | 0.0 | 614 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572000 | B7FHM9 | 95.268 | 317 | 1 | 2 | 1 | 303 | 1 | 317 | 0.0 | 611 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0118540 | Msa0572000 | 0.820581 | 6.359172e-53 | -8.615850e-47 |
| Msa0183870 | Msa0572000 | 0.803856 | 2.855500e-49 | -8.615850e-47 |
| Msa0187260 | Msa0572000 | 0.819751 | 9.851479e-53 | -8.615850e-47 |
| Msa0399760 | Msa0572000 | 0.811046 | 8.513270e-51 | -8.615850e-47 |
| Msa0244970 | Msa0572000 | 0.805745 | 1.150639e-49 | -8.615850e-47 |
| Msa0524600 | Msa0572000 | 0.803625 | 3.188088e-49 | -8.615850e-47 |
| Msa0526330 | Msa0572000 | 0.820866 | 5.469912e-53 | -8.615850e-47 |
| Msa0555640 | Msa0572000 | 0.800287 | 1.546827e-48 | -8.615850e-47 |
| Msa0572000 | Msa0609220 | 0.806675 | 7.331532e-50 | -8.615850e-47 |
| Msa0572000 | Msa0703730 | 0.800332 | 1.514926e-48 | -8.615850e-47 |
| Msa0572000 | Msa0707010 | 0.803852 | 2.860666e-49 | -8.615850e-47 |
| Msa0572000 | Msa0830210 | 0.821943 | 3.085198e-53 | -8.615850e-47 |
| Msa0572000 | Msa1031790 | 0.801967 | 7.012701e-49 | -8.615850e-47 |
| Msa0572000 | Msa1127430 | 0.814142 | 1.791388e-51 | -8.615850e-47 |
| Msa0572000 | Msa1200320 | 0.814501 | 1.492345e-51 | -8.615850e-47 |
| Msa0572000 | Msa1205480 | 0.810003 | 1.430527e-50 | -8.615850e-47 |
| Msa0572000 | Msa1336040 | 0.816151 | 6.410681e-52 | -8.615850e-47 |
| Msa0572000 | Msa1381650 | 0.801633 | 8.211183e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572000 | MtrunA17_Chr8g0387541 | 95.268 | 317 | 1 | 2 | 1 | 303 | 1 | 317 | 0.0 | 611 |
| Msa0572000 | MtrunA17_Chr1g0190121 | 74.922 | 319 | 64 | 4 | 1 | 304 | 1 | 318 | 3.05e-180 | 500 |
| Msa0572000 | MtrunA17_Chr7g0274931 | 73.981 | 319 | 67 | 4 | 1 | 304 | 1 | 318 | 2.33e-177 | 493 |
| Msa0572000 | MtrunA17_Chr5g0436061 | 74.510 | 306 | 64 | 2 | 1 | 292 | 17 | 322 | 1.66e-173 | 483 |
| Msa0572000 | MtrunA17_Chr1g0186811 | 71.111 | 315 | 75 | 4 | 1 | 300 | 1 | 314 | 4.99e-169 | 471 |
| Msa0572000 | MtrunA17_Chr1g0167751 | 71.290 | 310 | 73 | 4 | 1 | 295 | 1 | 309 | 1.29e-164 | 460 |
| Msa0572000 | MtrunA17_Chr7g0272371 | 70.323 | 310 | 71 | 4 | 1 | 296 | 5 | 307 | 1.02e-162 | 455 |
| Msa0572000 | MtrunA17_Chr8g0334361 | 73.356 | 289 | 62 | 3 | 6 | 280 | 4 | 291 | 1.25e-159 | 446 |
| Msa0572000 | MtrunA17_Chr8g0378521 | 44.108 | 297 | 142 | 4 | 6 | 287 | 9 | 296 | 2.57e-87 | 263 |
| Msa0572000 | MtrunA17_Chr7g0268381 | 45.652 | 276 | 134 | 3 | 27 | 287 | 27 | 301 | 1.29e-86 | 262 |
| Msa0572000 | MtrunA17_Chr5g0445011 | 43.206 | 287 | 145 | 3 | 27 | 298 | 17 | 300 | 3.62e-85 | 258 |
| Msa0572000 | MtrunA17_Chr8g0364041 | 45.660 | 265 | 128 | 3 | 27 | 276 | 20 | 283 | 1.18e-83 | 254 |
| Msa0572000 | MtrunA17_Chr5g0415261 | 46.038 | 265 | 127 | 3 | 27 | 276 | 20 | 283 | 1.23e-83 | 254 |
| Msa0572000 | MtrunA17_Chr1g0165341 | 39.871 | 311 | 158 | 7 | 6 | 300 | 3 | 300 | 1.41e-82 | 251 |
| Msa0572000 | MtrunA17_Chr2g0322121 | 47.794 | 272 | 114 | 5 | 23 | 266 | 660 | 931 | 1.02e-75 | 249 |
| Msa0572000 | MtrunA17_Chr4g0025471 | 43.648 | 307 | 143 | 6 | 3 | 279 | 643 | 949 | 7.89e-74 | 244 |
| Msa0572000 | MtrunA17_Chr3g0097231 | 41.115 | 287 | 144 | 7 | 3 | 269 | 168 | 449 | 3.29e-63 | 207 |
| Msa0572000 | MtrunA17_Chr2g0330311 | 39.649 | 285 | 145 | 8 | 3 | 266 | 195 | 473 | 7.08e-60 | 199 |
| Msa0572000 | MtrunA17_Chr2g0330331 | 38.754 | 289 | 151 | 8 | 2 | 269 | 32 | 315 | 1.52e-55 | 183 |
| Msa0572000 | MtrunA17_Chr1g0196211 | 44.444 | 180 | 76 | 5 | 109 | 265 | 2 | 180 | 2.05e-42 | 146 |
| Msa0572000 | MtrunA17_Chr8g0369981 | 30.476 | 315 | 140 | 11 | 34 | 269 | 100 | 414 | 1.88e-30 | 119 |
| Msa0572000 | MtrunA17_Chr1g0196221 | 46.296 | 108 | 50 | 2 | 9 | 108 | 528 | 635 | 2.72e-24 | 102 |
| Msa0572000 | MtrunA17_Chr2g0330011 | 36.975 | 119 | 65 | 4 | 32 | 141 | 232 | 349 | 2.34e-16 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572000 | AT1G64040.1 | 81.388 | 317 | 45 | 2 | 1 | 303 | 1 | 317 | 0.0 | 546 |
| Msa0572000 | AT4G11240.1 | 79.180 | 317 | 52 | 2 | 1 | 303 | 1 | 317 | 0.0 | 536 |
| Msa0572000 | AT2G29400.1 | 77.409 | 301 | 51 | 4 | 1 | 285 | 15 | 314 | 2.35e-174 | 485 |
| Msa0572000 | AT2G39840.1 | 75.415 | 301 | 60 | 2 | 1 | 287 | 14 | 314 | 2.41e-171 | 478 |
| Msa0572000 | AT5G59160.3 | 74.172 | 302 | 63 | 3 | 1 | 287 | 9 | 310 | 6.95e-168 | 468 |
| Msa0572000 | AT5G59160.2 | 74.172 | 302 | 63 | 3 | 1 | 287 | 9 | 310 | 6.95e-168 | 468 |
| Msa0572000 | AT5G59160.1 | 74.172 | 302 | 63 | 3 | 1 | 287 | 9 | 310 | 6.95e-168 | 468 |
| Msa0572000 | AT3G46820.1 | 73.179 | 302 | 66 | 3 | 1 | 287 | 9 | 310 | 2.23e-165 | 462 |
| Msa0572000 | AT5G43380.1 | 68.987 | 316 | 83 | 3 | 1 | 302 | 1 | 315 | 2.64e-162 | 455 |
| Msa0572000 | AT5G43380.3 | 68.987 | 316 | 83 | 3 | 1 | 302 | 1 | 315 | 6.27e-162 | 454 |
| Msa0572000 | AT5G43380.2 | 68.987 | 316 | 83 | 3 | 1 | 302 | 1 | 315 | 7.98e-162 | 454 |
| Msa0572000 | AT3G05580.1 | 72.297 | 296 | 65 | 3 | 1 | 282 | 9 | 301 | 6.90e-159 | 446 |
| Msa0572000 | AT5G27840.1 | 71.284 | 296 | 68 | 3 | 1 | 282 | 9 | 301 | 8.78e-159 | 446 |
| Msa0572000 | AT5G27840.4 | 69.903 | 309 | 71 | 4 | 1 | 290 | 9 | 314 | 1.21e-158 | 446 |
| Msa0572000 | AT5G27840.2 | 71.284 | 296 | 68 | 3 | 1 | 282 | 9 | 301 | 1.28e-158 | 445 |
| Msa0572000 | AT5G27840.3 | 71.284 | 296 | 68 | 3 | 1 | 282 | 9 | 301 | 1.28e-158 | 445 |
| Msa0572000 | AT3G05580.2 | 70.850 | 247 | 55 | 3 | 1 | 233 | 9 | 252 | 2.18e-126 | 361 |
| Msa0572000 | AT2G42500.1 | 44.781 | 297 | 140 | 4 | 6 | 287 | 14 | 301 | 8.04e-88 | 265 |
| Msa0572000 | AT5G55260.1 | 44.928 | 276 | 137 | 2 | 27 | 287 | 17 | 292 | 4.61e-87 | 263 |
| Msa0572000 | AT3G58500.1 | 44.108 | 297 | 142 | 4 | 6 | 287 | 14 | 301 | 1.69e-86 | 262 |
| Msa0572000 | AT4G26720.1 | 41.901 | 284 | 150 | 2 | 19 | 287 | 9 | 292 | 4.10e-85 | 258 |
| Msa0572000 | AT1G10430.2 | 44.056 | 286 | 136 | 4 | 6 | 276 | 7 | 283 | 3.45e-84 | 256 |
| Msa0572000 | AT1G10430.1 | 44.056 | 286 | 136 | 4 | 6 | 276 | 7 | 283 | 3.45e-84 | 256 |
| Msa0572000 | AT1G59830.1 | 44.406 | 286 | 135 | 4 | 6 | 276 | 7 | 283 | 5.56e-84 | 255 |
| Msa0572000 | AT1G69960.1 | 44.906 | 265 | 130 | 3 | 27 | 276 | 21 | 284 | 5.81e-83 | 253 |
| Msa0572000 | AT1G50370.1 | 39.871 | 311 | 158 | 7 | 6 | 300 | 3 | 300 | 1.01e-82 | 252 |
| Msa0572000 | AT3G19980.1 | 39.871 | 311 | 158 | 7 | 6 | 300 | 3 | 300 | 1.50e-82 | 251 |
| Msa0572000 | AT5G55260.2 | 39.117 | 317 | 137 | 3 | 27 | 287 | 17 | 333 | 1.49e-78 | 243 |
| Msa0572000 | AT2G27210.2 | 47.794 | 272 | 114 | 5 | 23 | 266 | 666 | 937 | 5.02e-76 | 251 |
| Msa0572000 | AT2G27210.1 | 47.794 | 272 | 114 | 5 | 23 | 266 | 671 | 942 | 5.26e-76 | 251 |
| Msa0572000 | AT1G08420.2 | 47.426 | 272 | 115 | 5 | 23 | 266 | 677 | 948 | 3.68e-75 | 248 |
| Msa0572000 | AT1G08420.1 | 47.426 | 272 | 115 | 5 | 23 | 266 | 682 | 953 | 3.85e-75 | 248 |
| Msa0572000 | AT4G03080.1 | 43.709 | 302 | 138 | 7 | 9 | 279 | 530 | 830 | 1.91e-74 | 245 |
| Msa0572000 | AT1G59830.2 | 45.600 | 250 | 112 | 4 | 6 | 240 | 7 | 247 | 2.89e-73 | 226 |
| Msa0572000 | AT1G03445.2 | 41.196 | 301 | 140 | 8 | 3 | 268 | 446 | 744 | 4.78e-67 | 223 |
| Msa0572000 | AT1G03445.1 | 41.196 | 301 | 140 | 8 | 3 | 268 | 446 | 744 | 4.82e-67 | 224 |
| Msa0572000 | AT2G42500.2 | 46.188 | 223 | 104 | 3 | 80 | 287 | 33 | 254 | 1.83e-66 | 209 |
| Msa0572000 | AT2G42810.1 | 39.649 | 285 | 146 | 7 | 2 | 266 | 170 | 448 | 1.85e-59 | 197 |
| Msa0572000 | AT2G42810.5 | 39.649 | 285 | 146 | 7 | 2 | 266 | 170 | 448 | 1.85e-59 | 197 |
| Msa0572000 | AT2G42810.4 | 39.649 | 285 | 146 | 7 | 2 | 266 | 170 | 448 | 1.85e-59 | 197 |
| Msa0572000 | AT2G42810.2 | 40.000 | 285 | 145 | 7 | 2 | 266 | 224 | 502 | 3.43e-59 | 198 |
| Msa0572000 | AT2G42810.3 | 39.649 | 285 | 146 | 7 | 2 | 266 | 223 | 501 | 3.63e-59 | 198 |
| Msa0572000 | AT2G42500.4 | 43.452 | 168 | 78 | 3 | 6 | 164 | 14 | 173 | 2.19e-40 | 139 |
| Msa0572000 | AT2G42500.5 | 42.857 | 175 | 81 | 4 | 6 | 171 | 14 | 178 | 2.53e-40 | 139 |
| Msa0572000 | AT2G42500.3 | 44.375 | 160 | 72 | 3 | 6 | 156 | 14 | 165 | 1.26e-39 | 137 |
| Msa0572000 | AT5G63870.1 | 29.924 | 264 | 121 | 8 | 56 | 255 | 79 | 342 | 1.30e-26 | 108 |
| Msa0572000 | AT5G63870.2 | 29.924 | 264 | 121 | 8 | 56 | 255 | 79 | 342 | 1.30e-26 | 108 |
| Msa0572000 | AT5G63870.3 | 32.258 | 186 | 86 | 5 | 56 | 201 | 79 | 264 | 9.39e-22 | 93.6 |
| Msa0572000 | AT1G48120.1 | 27.483 | 302 | 133 | 9 | 34 | 255 | 630 | 925 | 4.47e-20 | 91.3 |
| Msa0572000 | AT5G10900.2 | 29.119 | 261 | 131 | 11 | 56 | 264 | 225 | 483 | 7.26e-19 | 87.4 |
| Msa0572000 | AT5G10900.3 | 29.119 | 261 | 131 | 11 | 56 | 264 | 225 | 483 | 8.88e-19 | 87.0 |
| Msa0572000 | AT5G10900.1 | 29.119 | 261 | 131 | 11 | 56 | 264 | 225 | 483 | 1.07e-18 | 86.7 |
Find 78 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTCCATCAATCGTATTTA+TGG | 0.118761 | 4_1:+74804773 | Msa0572000:CDS |
| GAAGCAAATTACTTATTTCT+TGG | 0.231778 | 4_1:+74804641 | Msa0572000:CDS |
| TCAAACTCACCACAGTAATT+TGG | 0.239332 | 4_1:-74805804 | None:intergenic |
| ACCTTATGAGGTGGAGTTCC+AGG | 0.245870 | 4_1:-74805936 | None:intergenic |
| TATCGGAAGACTTCAGTATC+TGG | 0.265509 | 4_1:-74805875 | None:intergenic |
| TCCAACTTTGCCCTTCTTAT+CGG | 0.312310 | 4_1:-74805892 | None:intergenic |
| CCATGATCTGGCACATCAAT+AGG | 0.319380 | 4_1:-74804965 | None:intergenic |
| AGAAATAAGTAATTTGCTTC+AGG | 0.320791 | 4_1:-74804638 | None:intergenic |
| ATTGAAGATGTATCATCAAC+TGG | 0.347192 | 4_1:+74802965 | Msa0572000:five_prime_UTR |
| TGATTGCTTTAATTGTTTAC+CGG | 0.357293 | 4_1:+74804850 | Msa0572000:CDS |
| GCTTCAAGTTCAAGAAGATT+AGG | 0.359782 | 4_1:-74803292 | None:intergenic |
| AAGAAGGGCAAAGTTGGATT+TGG | 0.364950 | 4_1:+74805897 | Msa0572000:CDS |
| CGTATTCGAACAGCCTTAAA+AGG | 0.372745 | 4_1:-74804604 | None:intergenic |
| AGGAGAACTTCTTTCTTCTC+AGG | 0.373373 | 4_1:+74804735 | Msa0572000:CDS |
| CATCCCTCGAGATCTTTATC+AGG | 0.394159 | 4_1:-74805016 | None:intergenic |
| GCCTTCTCTGCGACCTTCTG+TGG | 0.394394 | 4_1:+74804987 | Msa0572000:CDS |
| GCCTTAAAAGGTCTGAAAAC+TGG | 0.396799 | 4_1:-74804592 | None:intergenic |
| GATTGATGGAGGCGCATTCA+TGG | 0.439246 | 4_1:-74804763 | None:intergenic |
| GAAACCATAAATACGATTGA+TGG | 0.442568 | 4_1:-74804777 | None:intergenic |
| TACAGTCACCAGCTTACGTT+TGG | 0.445882 | 4_1:-74805772 | None:intergenic |
| GATGTATCATCAACTGGGCT+AGG | 0.451995 | 4_1:+74802971 | Msa0572000:five_prime_UTR |
| ACCTGGAACTCCACCTCATA+AGG | 0.452558 | 4_1:+74805935 | Msa0572000:CDS |
| GAAGCTCCTATCAAAATTTG+TGG | 0.455039 | 4_1:+74803310 | Msa0572000:CDS |
| CCTATTGATGTGCCAGATCA+TGG | 0.458791 | 4_1:+74804965 | Msa0572000:CDS |
| AAAAGGAATTAAATAAAGTA+AGG | 0.468373 | 4_1:+74802906 | None:intergenic |
| CCCACAGAAGGTCGCAGAGA+AGG | 0.474160 | 4_1:-74804988 | None:intergenic |
| ACTGAAGTCTTCCGATAAGA+AGG | 0.476810 | 4_1:+74805881 | Msa0572000:CDS |
| AGAAGATCCTTTGCATGCAT+GGG | 0.477596 | 4_1:+74804891 | Msa0572000:CDS |
| TCGCAGAGAAGGCCATGATC+TGG | 0.483085 | 4_1:-74804977 | None:intergenic |
| CATGATCTGGCACATCAATA+GGG | 0.484326 | 4_1:-74804964 | None:intergenic |
| TCCGATAAGAAGGGCAAAGT+TGG | 0.484368 | 4_1:+74805891 | Msa0572000:CDS |
| GAGAGAAACGCGAAACGGAA+AGG | 0.491445 | 4_1:-74803078 | None:intergenic |
| GGAGAACTTCTTTCTTCTCA+GGG | 0.491751 | 4_1:+74804736 | Msa0572000:CDS |
| AATAAGTAATTTGCTTCAGG+TGG | 0.494184 | 4_1:-74804635 | None:intergenic |
| GCGTCCCAGGTTGTAGAAGA+TGG | 0.498692 | 4_1:+74805735 | Msa0572000:intron |
| AAAGAGAGAGAAACGCGAAA+CGG | 0.499054 | 4_1:-74803083 | None:intergenic |
| GAGAAGATCCTTTGCATGCA+TGG | 0.499556 | 4_1:+74804890 | Msa0572000:CDS |
| TGATAAAGATCTCGAGGGAT+GGG | 0.500185 | 4_1:+74805018 | Msa0572000:CDS |
| TCAGCTTTGTACTTCTGCTA+AGG | 0.513143 | 4_1:+74803252 | Msa0572000:CDS |
| TCTCTTGGAGATGGATGATA+CGG | 0.513600 | 4_1:+74803144 | Msa0572000:exon |
| CCTTCTCTGCGACCTTCTGT+GGG | 0.527902 | 4_1:+74804988 | Msa0572000:CDS |
| CTGATAAAGATCTCGAGGGA+TGG | 0.534435 | 4_1:+74805017 | Msa0572000:CDS |
| TTTAAGGCTGTTCGAATACG+GGG | 0.546220 | 4_1:+74804607 | Msa0572000:CDS |
| AAAGGTCTGAAAACTGGCCA+TGG | 0.549539 | 4_1:-74804586 | None:intergenic |
| TCTGATATGTAGAGCTCACC+AGG | 0.551295 | 4_1:+74805117 | Msa0572000:CDS |
| CTCAGTCGTTTACAAGAAGG+AGG | 0.558503 | 4_1:+74803005 | Msa0572000:five_prime_UTR |
| GTTTACAAGAAGGAGGGAAA+CGG | 0.565321 | 4_1:+74803012 | Msa0572000:five_prime_UTR |
| TTGTTTGCAGGAGATGTCCA+TGG | 0.568088 | 4_1:+74804569 | Msa0572000:intron |
| TCATCTACTAGAGCAGCAAC+CGG | 0.568940 | 4_1:-74804869 | None:intergenic |
| TGCATATAAAATCAAATACA+AGG | 0.570871 | 4_1:+74804715 | Msa0572000:CDS |
| GATAAAGATCTCGAGGGATG+GGG | 0.570941 | 4_1:+74805019 | Msa0572000:CDS |
| ATGTATCATCAACTGGGCTA+GGG | 0.572489 | 4_1:+74802972 | Msa0572000:five_prime_UTR |
| AAACTCAGTCGTTTACAAGA+AGG | 0.574886 | 4_1:+74803002 | Msa0572000:five_prime_UTR |
| TGTGGTGAGTTTGATAATGC+CGG | 0.578788 | 4_1:+74805813 | Msa0572000:CDS |
| CTTGGAGATTATGTTGATCG+TGG | 0.584311 | 4_1:+74804659 | Msa0572000:CDS |
| ATCCACACTCATCATAGCAC+CGG | 0.591401 | 4_1:-74805832 | None:intergenic |
| GGCAATAACTCATCAAGACC+TGG | 0.595098 | 4_1:+74805918 | Msa0572000:CDS |
| TATCAGGATCAGCCCACAGA+AGG | 0.599205 | 4_1:-74805000 | None:intergenic |
| CTGAAGTCTTCCGATAAGAA+GGG | 0.604771 | 4_1:+74805882 | Msa0572000:CDS |
| TGATCCTGATAAAGATCTCG+AGG | 0.605585 | 4_1:+74805012 | Msa0572000:CDS |
| ATAAGTAATTTGCTTCAGGT+GGG | 0.616465 | 4_1:-74804634 | None:intergenic |
| GAAGATCCTTTGCATGCATG+GGG | 0.624730 | 4_1:+74804892 | Msa0572000:CDS |
| TACAAGAAGGAGGGAAACGG+CGG | 0.626522 | 4_1:+74803015 | Msa0572000:five_prime_UTR |
| TTGAAGATGTATCATCAACT+GGG | 0.634964 | 4_1:+74802966 | Msa0572000:five_prime_UTR |
| AAGCTTGTATCAGCGAAGAA+CGG | 0.636419 | 4_1:+74803187 | Msa0572000:CDS |
| GTTTCTACGATGAGTGCAAG+AGG | 0.643121 | 4_1:+74804795 | Msa0572000:CDS |
| AAGCTGACGAATATCAGCCT+CGG | 0.647938 | 4_1:-74803236 | None:intergenic |
| ACCATAAATACGATTGATGG+AGG | 0.650130 | 4_1:-74804774 | None:intergenic |
| TCAGTCGTTTACAAGAAGGA+GGG | 0.661164 | 4_1:+74803006 | Msa0572000:five_prime_UTR |
| GAGAACTTCTTTCTTCTCAG+GGG | 0.674269 | 4_1:+74804737 | Msa0572000:CDS |
| TGCCGGTGCTATGATGAGTG+TGG | 0.686901 | 4_1:+74805830 | Msa0572000:CDS |
| TTAAGGCTGTTCGAATACGG+GGG | 0.692495 | 4_1:+74804608 | Msa0572000:CDS |
| TCTACGATGAGTGCAAGAGG+AGG | 0.698302 | 4_1:+74804798 | Msa0572000:CDS |
| GGATGGGGAGAAAATGATCG+AGG | 0.699695 | 4_1:+74805034 | Msa0572000:CDS |
| TTCTCTGCACCAAATTACTG+TGG | 0.705308 | 4_1:+74805795 | Msa0572000:CDS |
| GATCCTGATAAAGATCTCGA+GGG | 0.709098 | 4_1:+74805013 | Msa0572000:CDS |
| AAGATCCTTTGCATGCATGG+GGG | 0.732102 | 4_1:+74804893 | Msa0572000:CDS |
| GAAGCAAGTTCATCTTACCG+AGG | 0.743441 | 4_1:+74803219 | Msa0572000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAGTATTTTCTAATTAT+TGG | - | chr4_1:74814628-74814647 | None:intergenic | 10.0% |
| !! | AATAATTAGAAAATACTTAT+TGG | + | chr4_1:74814627-74814646 | Msa0572000:intron | 10.0% |
| !! | CTTATAAAGTTCAAAAATTA+GGG | - | chr4_1:74814398-74814417 | None:intergenic | 15.0% |
| !!! | AGAATATTTTGTTATATATG+TGG | + | chr4_1:74814431-74814450 | Msa0572000:intron | 15.0% |
| !!! | AATTTTTTGAGTTATGTTTT+TGG | + | chr4_1:74814749-74814768 | Msa0572000:intron | 15.0% |
| !!! | ATTTTTTGAGTTATGTTTTT+GGG | + | chr4_1:74814750-74814769 | Msa0572000:intron | 15.0% |
| !!! | TTGAATGATTTTCATGTTTT+TGG | + | chr4_1:74814349-74814368 | Msa0572000:intron | 20.0% |
| !! | CCTTATAAAGTTCAAAAATT+AGG | - | chr4_1:74814399-74814418 | None:intergenic | 20.0% |
| !!! | CCTAATTTTTGAACTTTATA+AGG | + | chr4_1:74814396-74814415 | Msa0572000:intron | 20.0% |
| !! | ATAAGTTCTTGGTAATAGTT+TGG | + | chr4_1:74814674-74814693 | Msa0572000:intron | 25.0% |
| ! | TTGATTCTATTACAAATCTG+AGG | - | chr4_1:74814730-74814749 | None:intergenic | 25.0% |
| !!! | TTTTGGGTTTATTATCATGT+TGG | + | chr4_1:74814766-74814785 | Msa0572000:intron | 25.0% |
| !! | TTTGGGTTTATTATCATGTT+GGG | + | chr4_1:74814767-74814786 | Msa0572000:intron | 25.0% |
| ATTGAAGATGTATCATCAAC+TGG | + | chr4_1:74802965-74802984 | Msa0572000:five_prime_UTR | 30.0% | |
| TTGAAGATGTATCATCAACT+GGG | + | chr4_1:74802966-74802985 | Msa0572000:five_prime_UTR | 30.0% | |
| ACTTTATAAGGAATTGACAC+AGG | + | chr4_1:74814408-74814427 | Msa0572000:intron | 30.0% | |
| TTCAAAACTAGCATATCAGT+TGG | - | chr4_1:74814580-74814599 | None:intergenic | 30.0% | |
| ACCAAGAACTTATATGATCA+AGG | - | chr4_1:74814667-74814686 | None:intergenic | 30.0% | |
| AGATTTAAGCAGTTACTAAC+AGG | - | chr4_1:74814801-74814820 | None:intergenic | 30.0% | |
| ! | AACTGCTTAAATCTTGTTGT+AGG | + | chr4_1:74814807-74814826 | Msa0572000:intron | 30.0% |
| GCCTTGATCATATAAGTTCT+TGG | + | chr4_1:74814663-74814682 | Msa0572000:intron | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 74802917 | 74814834 | 74802917 | ID=Msa0572000;Name=Msa0572000 |
| chr4_1 | mRNA | 74802917 | 74814834 | 74802917 | ID=Msa0572000-mRNA-1;Parent=Msa0572000;Name=Msa0572000-mRNA-1;_AED=0.18;_eAED=0.18;_QI=237|0.4|0.33|0.83|0.4|0.33|6|0|305 |
| chr4_1 | exon | 74802917 | 74803331 | 74802917 | ID=Msa0572000-mRNA-1:exon:25862;Parent=Msa0572000-mRNA-1 |
| chr4_1 | exon | 74804579 | 74804826 | 74804579 | ID=Msa0572000-mRNA-1:exon:25863;Parent=Msa0572000-mRNA-1 |
| chr4_1 | exon | 74804842 | 74805057 | 74804842 | ID=Msa0572000-mRNA-1:exon:25864;Parent=Msa0572000-mRNA-1 |
| chr4_1 | exon | 74805085 | 74805138 | 74805085 | ID=Msa0572000-mRNA-1:exon:25865;Parent=Msa0572000-mRNA-1 |
| chr4_1 | exon | 74805744 | 74805956 | 74805744 | ID=Msa0572000-mRNA-1:exon:25866;Parent=Msa0572000-mRNA-1 |
| chr4_1 | exon | 74814829 | 74814834 | 74814829 | ID=Msa0572000-mRNA-1:exon:25867;Parent=Msa0572000-mRNA-1 |
| chr4_1 | five_prime_UTR | 74802917 | 74803153 | 74802917 | ID=Msa0572000-mRNA-1:five_prime_utr;Parent=Msa0572000-mRNA-1 |
| chr4_1 | CDS | 74803154 | 74803331 | 74803154 | ID=Msa0572000-mRNA-1:cds;Parent=Msa0572000-mRNA-1 |
| chr4_1 | CDS | 74804579 | 74804826 | 74804579 | ID=Msa0572000-mRNA-1:cds;Parent=Msa0572000-mRNA-1 |
| chr4_1 | CDS | 74804842 | 74805057 | 74804842 | ID=Msa0572000-mRNA-1:cds;Parent=Msa0572000-mRNA-1 |
| chr4_1 | CDS | 74805085 | 74805138 | 74805085 | ID=Msa0572000-mRNA-1:cds;Parent=Msa0572000-mRNA-1 |
| chr4_1 | CDS | 74805744 | 74805956 | 74805744 | ID=Msa0572000-mRNA-1:cds;Parent=Msa0572000-mRNA-1 |
| chr4_1 | CDS | 74814829 | 74814834 | 74814829 | ID=Msa0572000-mRNA-1:cds;Parent=Msa0572000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
