AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0188030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188030 MtrunA17_Chr2g0290711 97.571 247 6 0 1 247 96 342 0.0 506
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188030 AT5G47770.1 81.377 247 46 0 1 247 138 384 2.77e-155 437
Msa0188030 AT4G17190.2 80.162 247 49 0 1 247 1 247 4.41e-153 426
Msa0188030 AT4G17190.3 80.162 247 49 0 1 247 59 305 6.08e-152 425
Msa0188030 AT4G17190.1 80.162 247 49 0 1 247 96 342 1.12e-151 426

Find 110 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCACCTTTCCAATTGTTTC+AGG 0.147131 2_1:-2997923 None:intergenic
TCTCATTCAGGATGATTATT+TGG 0.171673 2_1:+2997886 Msa0188030:intron
TTGATCTGCTGTTTAATTAT+TGG 0.186821 2_1:+2999134 Msa0188030:three_prime_UTR
TGATCTGCTGTTTAATTATT+GGG 0.234251 2_1:+2999135 Msa0188030:three_prime_UTR
ATTCATCAATACAGGATTAT+AGG 0.253804 2_1:-2996150 None:intergenic
CATTTAAATGTTGTTGGTTA+TGG 0.256594 2_1:+2999537 Msa0188030:three_prime_UTR
CGAGATTAAGCTCGTTATAA+AGG 0.264877 2_1:-2998248 None:intergenic
TTACAGTGATAAGGTGTATT+TGG 0.274262 2_1:-2996624 None:intergenic
TCCTAAGGATACGCGGAATA+TGG 0.280724 2_1:-2996397 None:intergenic
TGTGAAAGAAACATAGAAAA+TGG 0.282768 2_1:+2999002 Msa0188030:three_prime_UTR
TCTTGTTCTTGACGACATTA+TGG 0.285569 2_1:+2996194 Msa0188030:exon
TGTAAACTGTCTTGTGATAC+TGG 0.291626 2_1:+2998892 Msa0188030:three_prime_UTR
AGCAGTATTGAAGTCCTTCT+TGG 0.310069 2_1:+2998694 Msa0188030:CDS
AAAATGGATAGAGGAAATAA+AGG 0.317304 2_1:+2999018 Msa0188030:three_prime_UTR
AAGTTTAACCAATCATATTA+AGG 0.319300 2_1:-2999083 None:intergenic
TGTGGTGAACAGGAGAACTA+TGG 0.319745 2_1:+2998189 Msa0188030:intron
GGTAGTTGCCTTAATATGAT+TGG 0.367346 2_1:+2999075 Msa0188030:three_prime_UTR
ATAATATAGCGAACAGTTGC+AGG 0.367448 2_1:+2999361 Msa0188030:three_prime_UTR
TTGACTATAGCAACATTTGC+AGG 0.368701 2_1:-2998222 None:intergenic
TTGAAGACTTTAAGTGCTCT+TGG 0.374180 2_1:+2998033 Msa0188030:CDS
TACTGCTTGAACTGCACTGC+TGG 0.374612 2_1:-2998678 None:intergenic
TCGGTACATTTAAATGTTGT+TGG 0.375382 2_1:+2999531 Msa0188030:three_prime_UTR
AACAATGATATACCCGAGTT+TGG 0.378439 2_1:+2999750 Msa0188030:three_prime_UTR
CAAAATTCCCAACATCATCT+TGG 0.403595 2_1:-2999569 None:intergenic
CAGATTTACACAAGTGCAGC+AGG 0.409098 2_1:+2999446 Msa0188030:three_prime_UTR
AAATGGATAGAGGAAATAAA+GGG 0.411938 2_1:+2999019 Msa0188030:three_prime_UTR
TTTATGTACTTACAGTGATA+AGG 0.412214 2_1:-2996633 None:intergenic
ATGGCTGACCACGACGTGTA+TGG 0.415175 2_1:-2996224 None:intergenic
GATGATTATTTGGATTGCTT+TGG 0.419545 2_1:+2997896 Msa0188030:CDS
GTTGAGTTTCAAACTGCTGC+TGG 0.420249 2_1:+2996554 Msa0188030:CDS
CAGAAGTAGATTTCCCTTGT+AGG 0.422258 2_1:+2998734 Msa0188030:exon
ATAGCAACATTTGCAGGATC+TGG 0.426079 2_1:-2998216 None:intergenic
TACTTAAGCCTTCCCCTGCT+CGG 0.432552 2_1:-2999489 None:intergenic
TTGCAATGAAGAACAAAAGA+AGG 0.435483 2_1:+2998079 Msa0188030:CDS
ACTAATGGCTGGTGAAGATC+TGG 0.438016 2_1:+2997683 Msa0188030:CDS
GCTGCAAAATTCATCAATAC+AGG 0.440620 2_1:-2996158 None:intergenic
TCCAACTCGAAATCAACATT+TGG 0.440685 2_1:-2999693 None:intergenic
ATCAATAATGGCTCCAAACT+CGG 0.443371 2_1:-2999763 None:intergenic
TGAAGACTTTAAGTGCTCTT+GGG 0.443835 2_1:+2998034 Msa0188030:CDS
TAGCGAACAGTTGCAGGAAC+AGG 0.445801 2_1:+2999367 Msa0188030:three_prime_UTR
GTCAGCTCCACACAGAAAAC+AGG 0.449743 2_1:-2998803 None:intergenic
TTTGGTGATCCTGAAACAAT+TGG 0.452410 2_1:+2997914 Msa0188030:CDS
TACACAAGTGCAGCAGGGTC+AGG 0.453632 2_1:+2999452 Msa0188030:three_prime_UTR
TCAAGAAGTTCAACATAATA+CGG 0.457431 2_1:-2996437 None:intergenic
GATGAAGTAAATAGAGAAGA+AGG 0.459696 2_1:+2999325 Msa0188030:three_prime_UTR
GAGATTAAGCTCGTTATAAA+GGG 0.459820 2_1:-2998247 None:intergenic
TTCCCTTGAAGTGTTTCCTA+AGG 0.467529 2_1:-2996412 None:intergenic
CATATTAAGGCAACTACCAT+TGG 0.471122 2_1:-2999070 None:intergenic
AAAGAACATTCTTGTTGAGA+TGG 0.473997 2_1:+2997722 Msa0188030:CDS
ACCATATTCCGCGTATCCTT+AGG 0.482141 2_1:+2996396 Msa0188030:CDS
TGCAGCAGGGTCAGGTTTGG+TGG 0.484303 2_1:+2999460 Msa0188030:three_prime_UTR
ACATTGTTTGAAGATGCAAT+AGG 0.485380 2_1:+2998767 Msa0188030:three_prime_UTR
TATCCTTAGGAAACACTTCA+AGG 0.492202 2_1:+2996409 Msa0188030:CDS
GAAACATAGAAAATGGATAG+AGG 0.503294 2_1:+2999009 Msa0188030:three_prime_UTR
TCAATAATGGCTCCAAACTC+GGG 0.505745 2_1:-2999762 None:intergenic
AACAGTTGCAGGAACAGGAT+TGG 0.506063 2_1:+2999372 Msa0188030:three_prime_UTR
ATGTTGGTATTTGTCCTACA+AGG 0.508631 2_1:-2998748 None:intergenic
AAAATAGTCTACAACCCTCT+TGG 0.515575 2_1:-2999718 None:intergenic
GGAATGATTGCTGCGAATGA+TGG 0.516786 2_1:+2996359 Msa0188030:CDS
GAATGTATAGGGTGCATTTG+CGG 0.518977 2_1:+2998604 Msa0188030:intron
TGGCTGACCACGACGTGTAT+GGG 0.519560 2_1:-2996223 None:intergenic
AAGAACATTCTTGTTGAGAT+GGG 0.521436 2_1:+2997723 Msa0188030:CDS
AAGTGCAGCAGGGTCAGGTT+TGG 0.526753 2_1:+2999457 Msa0188030:three_prime_UTR
TCCAAATGTTGATTTCGAGT+TGG 0.529065 2_1:+2999692 Msa0188030:three_prime_UTR
CCCGTGTGTGTGTCGGATAT+AGG 0.529858 2_1:+2999618 Msa0188030:three_prime_UTR
GTCGGATATAGGTGAAGCAT+TGG 0.538354 2_1:+2999629 Msa0188030:three_prime_UTR
ATTGCATCTTCAAACAATGT+TGG 0.539226 2_1:-2998764 None:intergenic
GTTGCAGGAACAGGATTGGC+AGG 0.540388 2_1:+2999376 Msa0188030:three_prime_UTR
CACGTCGTGGTCAGCCATGT+TGG 0.540988 2_1:+2996229 Msa0188030:CDS
GTGATAAGGTGTATTTGGAC+AGG 0.546327 2_1:-2996619 None:intergenic
TCGGATATAGGTGAAGCATT+GGG 0.547463 2_1:+2999630 Msa0188030:three_prime_UTR
ACCTATATCCGACACACACA+CGG 0.552322 2_1:-2999619 None:intergenic
TGTGCATCCAAGATGATGTT+GGG 0.557801 2_1:+2999562 Msa0188030:three_prime_UTR
GGTTGCATGTGCATTACTAA+TGG 0.564966 2_1:+2997668 Msa0188030:intron
ATGTTGGTTTAGAGTACCCA+AGG 0.567818 2_1:+2996245 Msa0188030:CDS
GTGGTGAACAGGAGAACTAT+GGG 0.570700 2_1:+2998190 Msa0188030:intron
TTGGGTGGTTGTGAAAGCAT+TGG 0.575145 2_1:+2998052 Msa0188030:CDS
TTATAACGAGCTTAATCTCG+AGG 0.575890 2_1:+2998251 Msa0188030:CDS
TTTCGAGTTGGATGCCAAGA+GGG 0.578061 2_1:+2999704 Msa0188030:three_prime_UTR
CCTATATCCGACACACACAC+GGG 0.579419 2_1:-2999618 None:intergenic
GCATGTGCATTACTAATGGC+TGG 0.579792 2_1:+2997672 Msa0188030:CDS
GTCAAGCCCCGTGTGTGTGT+CGG 0.584280 2_1:+2999611 Msa0188030:three_prime_UTR
AGACTTTAAGTGCTCTTGGG+TGG 0.587887 2_1:+2998037 Msa0188030:CDS
ACTGCTTGAACTGCACTGCT+GGG 0.591941 2_1:-2998677 None:intergenic
GTTTGTCACAAGCTATTCAG+TGG 0.598148 2_1:+2999652 Msa0188030:three_prime_UTR
ACTTCTTGATTTATTCAATG+AGG 0.600835 2_1:+2996451 Msa0188030:CDS
AAGATGCAATAGGAGCTAGC+TGG 0.605998 2_1:+2998777 Msa0188030:three_prime_UTR
TGTTGGTATTTGTCCTACAA+GGG 0.609648 2_1:-2998747 None:intergenic
TTATAGTAGCAAACTTCCAA+TGG 0.615369 2_1:+2999054 Msa0188030:three_prime_UTR
ATGTGCATCCAAGATGATGT+TGG 0.617601 2_1:+2999561 Msa0188030:three_prime_UTR
TTTGTCACAAGCTATTCAGT+GGG 0.618720 2_1:+2999653 Msa0188030:three_prime_UTR
TTGTGAAAACAAGGACGATG+TGG 0.619742 2_1:+2999589 Msa0188030:three_prime_UTR
TCAAGTTCAGAGATAGACCA+TGG 0.620633 2_1:+2998972 Msa0188030:three_prime_UTR
GATTTGATCACCACACTGGA+AGG 0.625103 2_1:+2996587 Msa0188030:CDS
ATCCTTAGGAAACACTTCAA+GGG 0.625790 2_1:+2996410 Msa0188030:CDS
TCTTGGCTAAAATTTACAAG+AGG 0.627016 2_1:+2998711 Msa0188030:CDS
GAATGTTCTTTACATCAACA+TGG 0.628388 2_1:-2997710 None:intergenic
TTCTTTCACAGGCAAAACCA+TGG 0.631764 2_1:-2998989 None:intergenic
GATCGATTTGATCACCACAC+TGG 0.647023 2_1:+2996583 Msa0188030:CDS
ACAGTTAACACTATATCAGA+CGG 0.649893 2_1:+2999423 Msa0188030:three_prime_UTR
ATTTCGAGTTGGATGCCAAG+AGG 0.662942 2_1:+2999703 Msa0188030:three_prime_UTR
AGATTTACACAAGTGCAGCA+GGG 0.679813 2_1:+2999447 Msa0188030:three_prime_UTR
TGATCCTGAAACAATTGGAA+AGG 0.681979 2_1:+2997919 Msa0188030:CDS
TTGGGTACTCTAAACCAACA+TGG 0.682317 2_1:-2996243 None:intergenic
GACAACTCCCATACACGTCG+TGG 0.685129 2_1:+2996216 Msa0188030:CDS
CTATATCCGACACACACACG+GGG 0.691294 2_1:-2999617 None:intergenic
AAGTGTTTCCTAAGGATACG+CGG 0.701381 2_1:-2996404 None:intergenic
GCTGGGATGAGCTTCAATCG+AGG 0.703794 2_1:-2998660 None:intergenic
TCTTGTACTGAACAATACGC+CGG 0.718334 2_1:-2997125 None:intergenic
GGGAAGGCTTAAGTACAACG+TGG 0.767317 2_1:+2999497 Msa0188030:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTACTTTTTTTTATTAA+TGG + chr2_1:2997426-2997445 Msa0188030:intron 10.0%
!!! ATATTATTTTTGTTGTTGTA+GGG + chr2_1:2996298-2996317 Msa0188030:intron 15.0%
!!! TTTTATGTTATTGTTAGTAT+TGG + chr2_1:2996718-2996737 Msa0188030:intron 15.0%
!! ATATAAAAAATGAAGATGAA+AGG - chr2_1:2997363-2997382 None:intergenic 15.0%
!! AAAATAAGTAATTTCGTTAA+GGG - chr2_1:2997549-2997568 None:intergenic 15.0%
!! AAAAATAAGTAATTTCGTTA+AGG - chr2_1:2997550-2997569 None:intergenic 15.0%
!! AAAGATAAATAACATAAAAC+AGG - chr2_1:2997624-2997643 None:intergenic 15.0%
!!! ATTTTTTTTTCCTTCTAAAA+TGG - chr2_1:2998317-2998336 None:intergenic 15.0%
!!! TATTTGAAATTTGAATGTAT+AGG + chr2_1:2998592-2998611 Msa0188030:intron 15.0%
!!! ATTTGAAATTTGAATGTATA+GGG + chr2_1:2998593-2998612 Msa0188030:intron 15.0%
!!! GATATTATTTTTGTTGTTGT+AGG + chr2_1:2996297-2996316 Msa0188030:intron 20.0%
!! CTACATCATAGAATAATTAA+AGG - chr2_1:2996336-2996355 None:intergenic 20.0%
!! CTTTAATTATTCTATGATGT+AGG + chr2_1:2996334-2996353 Msa0188030:intron 20.0%
!! AACCTAAAAGAAAATTAACA+CGG - chr2_1:2996537-2996556 None:intergenic 20.0%
!! ACTGTAAGTACATAAAAAAT+AGG + chr2_1:2996640-2996659 Msa0188030:intron 20.0%
!!! AATAGTTTTTTCTTTTTGCT+TGG - chr2_1:2996746-2996765 None:intergenic 20.0%
!!! TTTTCTTTTAGATACATACT+TGG + chr2_1:2996822-2996841 Msa0188030:intron 20.0%
!!! TTTTGTTGTTTTCCTTATTT+CGG + chr2_1:2997194-2997213 Msa0188030:intron 20.0%
!!! AGGTTAAACTACATTTTTAA+AGG - chr2_1:2997855-2997874 None:intergenic 20.0%
!! TGAATGAGAACAAAAAAATT+AGG - chr2_1:2997875-2997894 None:intergenic 20.0%
!!! TAATTTTTTTGTTCTCATTC+AGG + chr2_1:2997874-2997893 Msa0188030:intron 20.0%
!!! TTAACAGTTTAAAATTGTTG+CGG - chr2_1:2998367-2998386 None:intergenic 20.0%
!! GATATTCTTAAAAAAATGTG+TGG - chr2_1:2998511-2998530 None:intergenic 20.0%
!!! TTCTTAATAACTTTTCTTTC+TGG + chr2_1:2998921-2998940 Msa0188030:three_prime_UTR 20.0%
!! AAGTTTAACCAATCATATTA+AGG - chr2_1:2999086-2999105 None:intergenic 20.0%
! ATTCATCAATACAGGATTAT+AGG - chr2_1:2996153-2996172 None:intergenic 25.0%
!!! TTTTTTTTTCTCCAAACCTT+GGG - chr2_1:2996264-2996283 None:intergenic 25.0%
! TTTGGAGAAAAAAAAACATG+TGG + chr2_1:2996268-2996287 Msa0188030:intron 25.0%
! AATTATTCTATGATGTAGGT+TGG + chr2_1:2996338-2996357 Msa0188030:intron 25.0%
! TCAAGAAGTTCAACATAATA+CGG - chr2_1:2996440-2996459 None:intergenic 25.0%
! ACTTCTTGATTTATTCAATG+AGG + chr2_1:2996451-2996470 Msa0188030:CDS 25.0%
! ATTTCAGTGTTTGAATTATG+TGG + chr2_1:2996482-2996501 Msa0188030:intron 25.0%
! TTTATGTACTTACAGTGATA+AGG - chr2_1:2996636-2996655 None:intergenic 25.0%
! TGTCATATAAAACTGTTATC+TGG - chr2_1:2996772-2996791 None:intergenic 25.0%
!! GAATTTTACATTGTTCTTTC+AGG - chr2_1:2996928-2996947 None:intergenic 25.0%
! AGGAAGGTAAAAAGAATAAT+AGG - chr2_1:2997154-2997173 None:intergenic 25.0%
! AAGTATGATATAACACTTAC+AGG - chr2_1:2997174-2997193 None:intergenic 25.0%
! TAATTTCGTTAAGGGATTAA+AGG - chr2_1:2997541-2997560 None:intergenic 25.0%
! TTTGAAAAATCCTTTCACAA+TGG + chr2_1:2997589-2997608 Msa0188030:intron 25.0%
! AAGTCATTTCAGTTTAATCA+TGG - chr2_1:2998167-2998186 None:intergenic 25.0%
!! TAAACTGAAATGACTTTTTG+TGG + chr2_1:2998171-2998190 Msa0188030:intron 25.0%
!!! TAAATAACTGCCATTTTAGA+AGG + chr2_1:2998304-2998323 Msa0188030:intron 25.0%
! AAAATTGTTGCGGATAAAAT+TGG - chr2_1:2998357-2998376 None:intergenic 25.0%
! TGATAGTAAGATCAATGAAT+TGG - chr2_1:2998394-2998413 None:intergenic 25.0%
!!! TTTCTTACCATGTCTTTTTT+AGG + chr2_1:2998841-2998860 Msa0188030:three_prime_UTR 25.0%
!!! TTCTTACCATGTCTTTTTTA+GGG + chr2_1:2998842-2998861 Msa0188030:three_prime_UTR 25.0%
!!! ACTTTTCTTTCTGGAATATT+TGG + chr2_1:2998930-2998949 Msa0188030:three_prime_UTR 25.0%
!! ATTTTCTATGTTTCTTTCAC+AGG - chr2_1:2999003-2999022 None:intergenic 25.0%
! TGTGAAAGAAACATAGAAAA+TGG + chr2_1:2999002-2999021 Msa0188030:three_prime_UTR 25.0%
! AAAATGGATAGAGGAAATAA+AGG + chr2_1:2999018-2999037 Msa0188030:three_prime_UTR 25.0%
! AAATGGATAGAGGAAATAAA+GGG + chr2_1:2999019-2999038 Msa0188030:three_prime_UTR 25.0%
!!! GGTTAAACTTTTTTTTCACT+TGG + chr2_1:2999096-2999115 Msa0188030:three_prime_UTR 25.0%
!! TTGATCTGCTGTTTAATTAT+TGG + chr2_1:2999134-2999153 Msa0188030:three_prime_UTR 25.0%
!! TGATCTGCTGTTTAATTATT+GGG + chr2_1:2999135-2999154 Msa0188030:three_prime_UTR 25.0%
!! CATTTAAATGTTGTTGGTTA+TGG + chr2_1:2999537-2999556 Msa0188030:three_prime_UTR 25.0%
!!! GTTTTTTTTTCTCCAAACCT+TGG - chr2_1:2996265-2996284 None:intergenic 30.0%
! GACCGTGTTAATTTTCTTTT+AGG + chr2_1:2996532-2996551 Msa0188030:intron 30.0%
TTACAGTGATAAGGTGTATT+TGG - chr2_1:2996627-2996646 None:intergenic 30.0%
TTGTGTAGAACTTATGTTGA+TGG + chr2_1:2996670-2996689 Msa0188030:intron 30.0%
! CCAACATTTTACGTTTATCT+AGG - chr2_1:2996697-2996716 None:intergenic 30.0%
CCTAGATAAACGTAAAATGT+TGG + chr2_1:2996694-2996713 Msa0188030:intron 30.0%
TACATACTTGGCAATCTTTA+TGG + chr2_1:2996834-2996853 Msa0188030:intron 30.0%
TGTAAGATAGCAGAATAACA+AGG - chr2_1:2996876-2996895 None:intergenic 30.0%
! AATGCGTATGAGTTGAAATT+CGG + chr2_1:2996960-2996979 Msa0188030:intron 30.0%
ATGATATAACACTTACAGGA+AGG - chr2_1:2997170-2997189 None:intergenic 30.0%
!! TCTTTTAAGTGAAGACTCAT+TGG - chr2_1:2997407-2997426 None:intergenic 30.0%
GTTATATGTTCCATTGTGAA+AGG - chr2_1:2997602-2997621 None:intergenic 30.0%
! TATGTATCTCCTATTTTCAC+AGG + chr2_1:2997647-2997666 Msa0188030:intron 30.0%
GAATGTTCTTTACATCAACA+TGG - chr2_1:2997713-2997732 None:intergenic 30.0%
AAAGAACATTCTTGTTGAGA+TGG + chr2_1:2997722-2997741 Msa0188030:CDS 30.0%
AAGAACATTCTTGTTGAGAT+GGG + chr2_1:2997723-2997742 Msa0188030:CDS 30.0%
TCTCATTCAGGATGATTATT+TGG + chr2_1:2997886-2997905 Msa0188030:intron 30.0%
GATGATTATTTGGATTGCTT+TGG + chr2_1:2997896-2997915 Msa0188030:CDS 30.0%
AGTTCTTTATGAAACCAGAT+AGG + chr2_1:2998002-2998021 Msa0188030:intron 30.0%
TTGCAATGAAGAACAAAAGA+AGG + chr2_1:2998079-2998098 Msa0188030:CDS 30.0%
GAGATTAAGCTCGTTATAAA+GGG - chr2_1:2998250-2998269 None:intergenic 30.0%
AAATGGCAGTTATTTAGAAG+TGG - chr2_1:2998300-2998319 None:intergenic 30.0%
ACTATCATAGTTGTCAAATG+CGG + chr2_1:2998407-2998426 Msa0188030:intron 30.0%
!!! TTTTTTGAGAAGGCAAATGT+AGG + chr2_1:2998462-2998481 Msa0188030:intron 30.0%
!! CTTGTAAATTTTAGCCAAGA+AGG - chr2_1:2998711-2998730 None:intergenic 30.0%
TCTTGGCTAAAATTTACAAG+AGG + chr2_1:2998711-2998730 Msa0188030:CDS 30.0%
ATTGCATCTTCAAACAATGT+TGG - chr2_1:2998767-2998786 None:intergenic 30.0%
ACATTGTTTGAAGATGCAAT+AGG + chr2_1:2998767-2998786 Msa0188030:three_prime_UTR 30.0%
!!! TCTTACCATGTCTTTTTTAG+GGG + chr2_1:2998843-2998862 Msa0188030:three_prime_UTR 30.0%
GAAACATAGAAAATGGATAG+AGG + chr2_1:2999009-2999028 Msa0188030:three_prime_UTR 30.0%
TTATAGTAGCAAACTTCCAA+TGG + chr2_1:2999054-2999073 Msa0188030:three_prime_UTR 30.0%
GATGAAGTAAATAGAGAAGA+AGG + chr2_1:2999325-2999344 Msa0188030:three_prime_UTR 30.0%
ACAGTTAACACTATATCAGA+CGG + chr2_1:2999423-2999442 Msa0188030:three_prime_UTR 30.0%
TCGGTACATTTAAATGTTGT+TGG + chr2_1:2999531-2999550 Msa0188030:three_prime_UTR 30.0%
! TTGGGAATTTTGTGAAAACA+AGG + chr2_1:2999580-2999599 Msa0188030:three_prime_UTR 30.0%
!!! GGTTGTAGACTATTTTAACA+CGG + chr2_1:2999725-2999744 Msa0188030:three_prime_UTR 30.0%
!!! GTTGTAGACTATTTTAACAC+GGG + chr2_1:2999726-2999745 Msa0188030:three_prime_UTR 30.0%
GCTGCAAAATTCATCAATAC+AGG - chr2_1:2996161-2996180 None:intergenic 35.0%
TCTTGTTCTTGACGACATTA+TGG + chr2_1:2996194-2996213 Msa0188030:exon 35.0%
TATCCTTAGGAAACACTTCA+AGG + chr2_1:2996409-2996428 Msa0188030:CDS 35.0%
ATCCTTAGGAAACACTTCAA+GGG + chr2_1:2996410-2996429 Msa0188030:CDS 35.0%
! TATCTCTCTTTTGACACTGA+TGG - chr2_1:2996796-2996815 None:intergenic 35.0%
AACGACAAGAAACCGAAATA+AGG - chr2_1:2997209-2997228 None:intergenic 35.0%
TAAAGGAGTTGAGGAGATAA+AGG - chr2_1:2997524-2997543 None:intergenic 35.0%
TTAAGGGATTAAAGGAGTTG+AGG - chr2_1:2997533-2997552 None:intergenic 35.0%
!!! GGGAACATATTTTCAAGTTC+AGG + chr2_1:2997743-2997762 Msa0188030:CDS 35.0%
!! ACAGAATTCTAGAGTGTGAA+TGG - chr2_1:2997790-2997809 None:intergenic 35.0%
! TTTGGTGATCCTGAAACAAT+TGG + chr2_1:2997914-2997933 Msa0188030:CDS 35.0%
TGATCCTGAAACAATTGGAA+AGG + chr2_1:2997919-2997938 Msa0188030:CDS 35.0%
TTGAAGACTTTAAGTGCTCT+TGG + chr2_1:2998033-2998052 Msa0188030:CDS 35.0%
TGAAGACTTTAAGTGCTCTT+GGG + chr2_1:2998034-2998053 Msa0188030:CDS 35.0%
CATGGCAGAAAAAAAAGAGA+AGG - chr2_1:2998149-2998168 None:intergenic 35.0%
! AATGACTTTTTGTGGTGAAC+AGG + chr2_1:2998179-2998198 Msa0188030:intron 35.0%
TTGACTATAGCAACATTTGC+AGG - chr2_1:2998225-2998244 None:intergenic 35.0%
CGAGATTAAGCTCGTTATAA+AGG - chr2_1:2998251-2998270 None:intergenic 35.0%
TTATAACGAGCTTAATCTCG+AGG + chr2_1:2998251-2998270 Msa0188030:CDS 35.0%
TGTTGGTATTTGTCCTACAA+GGG - chr2_1:2998750-2998769 None:intergenic 35.0%
ATGTTGGTATTTGTCCTACA+AGG - chr2_1:2998751-2998770 None:intergenic 35.0%
!!! CTTACCATGTCTTTTTTAGG+GGG + chr2_1:2998844-2998863 Msa0188030:three_prime_UTR 35.0%
TGTAAACTGTCTTGTGATAC+TGG + chr2_1:2998892-2998911 Msa0188030:three_prime_UTR 35.0%
CATATTAAGGCAACTACCAT+TGG - chr2_1:2999073-2999092 None:intergenic 35.0%
GGTAGTTGCCTTAATATGAT+TGG + chr2_1:2999075-2999094 Msa0188030:three_prime_UTR 35.0%
ATAATATAGCGAACAGTTGC+AGG + chr2_1:2999361-2999380 Msa0188030:three_prime_UTR 35.0%
CAAAATTCCCAACATCATCT+TGG - chr2_1:2999572-2999591 None:intergenic 35.0%
TTTGTCACAAGCTATTCAGT+GGG + chr2_1:2999653-2999672 Msa0188030:three_prime_UTR 35.0%
!! TCCAAATGTTGATTTCGAGT+TGG + chr2_1:2999692-2999711 Msa0188030:three_prime_UTR 35.0%
TCCAACTCGAAATCAACATT+TGG - chr2_1:2999696-2999715 None:intergenic 35.0%
AAAATAGTCTACAACCCTCT+TGG - chr2_1:2999721-2999740 None:intergenic 35.0%
AACAATGATATACCCGAGTT+TGG + chr2_1:2999750-2999769 Msa0188030:three_prime_UTR 35.0%
TTGGGTACTCTAAACCAACA+TGG - chr2_1:2996246-2996265 None:intergenic 40.0%
!! ATGTTGGTTTAGAGTACCCA+AGG + chr2_1:2996245-2996264 Msa0188030:CDS 40.0%
AAGTGTTTCCTAAGGATACG+CGG - chr2_1:2996407-2996426 None:intergenic 40.0%
! TTCCCTTGAAGTGTTTCCTA+AGG - chr2_1:2996415-2996434 None:intergenic 40.0%
GTGATAAGGTGTATTTGGAC+AGG - chr2_1:2996622-2996641 None:intergenic 40.0%
GCTAGCTAGAAGAGAACAAT+AGG - chr2_1:2996992-2997011 None:intergenic 40.0%
TAGCTATGAACATCTTCTGC+AGG + chr2_1:2997100-2997119 Msa0188030:intron 40.0%
TCTTGTACTGAACAATACGC+CGG - chr2_1:2997128-2997147 None:intergenic 40.0%
! TAGCATTTTTCTCCTTTGCC+AGG + chr2_1:2997484-2997503 Msa0188030:intron 40.0%
CACATGCAACCTGTGAAAAT+AGG - chr2_1:2997659-2997678 None:intergenic 40.0%
GGTTGCATGTGCATTACTAA+TGG + chr2_1:2997668-2997687 Msa0188030:intron 40.0%
CTCACCTTTCCAATTGTTTC+AGG - chr2_1:2997926-2997945 None:intergenic 40.0%
CTTCAATATCTGTGCCTATC+TGG - chr2_1:2998019-2998038 None:intergenic 40.0%
AAAGAGAAGGAAAGAAGCAG+CGG - chr2_1:2998136-2998155 None:intergenic 40.0%
ATAGCAACATTTGCAGGATC+TGG - chr2_1:2998219-2998238 None:intergenic 40.0%
GGCAGTTATTTAGAAGTGGA+GGG - chr2_1:2998296-2998315 None:intergenic 40.0%
TGGCAGTTATTTAGAAGTGG+AGG - chr2_1:2998297-2998316 None:intergenic 40.0%
! TGGAGATGATCTTTCGCTTT+TGG - chr2_1:2998491-2998510 None:intergenic 40.0%
GAATGTATAGGGTGCATTTG+CGG + chr2_1:2998604-2998623 Msa0188030:intron 40.0%
! AGCAGTATTGAAGTCCTTCT+TGG + chr2_1:2998694-2998713 Msa0188030:CDS 40.0%
CAGAAGTAGATTTCCCTTGT+AGG + chr2_1:2998734-2998753 Msa0188030:exon 40.0%
ATCACCCCCTAAAAAAGACA+TGG - chr2_1:2998851-2998870 None:intergenic 40.0%
TCAAGTTCAGAGATAGACCA+TGG + chr2_1:2998972-2998991 Msa0188030:three_prime_UTR 40.0%
TTCTTTCACAGGCAAAACCA+TGG - chr2_1:2998992-2999011 None:intergenic 40.0%
! TTTTCACTTGGCTTCGTGTA+TGG + chr2_1:2999108-2999127 Msa0188030:three_prime_UTR 40.0%
AGATTTACACAAGTGCAGCA+GGG + chr2_1:2999447-2999466 Msa0188030:three_prime_UTR 40.0%
ATGTGCATCCAAGATGATGT+TGG + chr2_1:2999561-2999580 Msa0188030:three_prime_UTR 40.0%
TGTGCATCCAAGATGATGTT+GGG + chr2_1:2999562-2999581 Msa0188030:three_prime_UTR 40.0%
TTGTGAAAACAAGGACGATG+TGG + chr2_1:2999589-2999608 Msa0188030:three_prime_UTR 40.0%
! TCGGATATAGGTGAAGCATT+GGG + chr2_1:2999630-2999649 Msa0188030:three_prime_UTR 40.0%
GTTTGTCACAAGCTATTCAG+TGG + chr2_1:2999652-2999671 Msa0188030:three_prime_UTR 40.0%
! GGTTTAGAGTACCCAAGGTT+TGG + chr2_1:2996250-2996269 Msa0188030:intron 45.0%
GGAATGATTGCTGCGAATGA+TGG + chr2_1:2996359-2996378 Msa0188030:CDS 45.0%
ACCATATTCCGCGTATCCTT+AGG + chr2_1:2996396-2996415 Msa0188030:CDS 45.0%
TCCTAAGGATACGCGGAATA+TGG - chr2_1:2996400-2996419 None:intergenic 45.0%
GTTGAGTTTCAAACTGCTGC+TGG + chr2_1:2996554-2996573 Msa0188030:CDS 45.0%
!! GATCGATTTGATCACCACAC+TGG + chr2_1:2996583-2996602 Msa0188030:CDS 45.0%
GATTTGATCACCACACTGGA+AGG + chr2_1:2996587-2996606 Msa0188030:CDS 45.0%
! GTCTTTTTCTCCTTCCAGTG+TGG - chr2_1:2996600-2996619 None:intergenic 45.0%
GTTGAGGAGATAAAGGTCGA+GGG - chr2_1:2997517-2997536 None:intergenic 45.0%
AGTTGAGGAGATAAAGGTCG+AGG - chr2_1:2997518-2997537 None:intergenic 45.0%
GCATGTGCATTACTAATGGC+TGG + chr2_1:2997672-2997691 Msa0188030:CDS 45.0%
ACTAATGGCTGGTGAAGATC+TGG + chr2_1:2997683-2997702 Msa0188030:CDS 45.0%
TACTGTCTGTCACTATCAGC+TGG + chr2_1:2997823-2997842 Msa0188030:intron 45.0%
AATTGGAAAGGTGAGTGCAC+TGG + chr2_1:2997931-2997950 Msa0188030:intron 45.0%
ACTGGATCATGCATGGATAC+TGG + chr2_1:2997949-2997968 Msa0188030:intron 45.0%
AGACTTTAAGTGCTCTTGGG+TGG + chr2_1:2998037-2998056 Msa0188030:CDS 45.0%
! TTGGGTGGTTGTGAAAGCAT+TGG + chr2_1:2998052-2998071 Msa0188030:CDS 45.0%
AAGAGAAGGAAAGAAGCAGC+GGG - chr2_1:2998135-2998154 None:intergenic 45.0%
TGTGGTGAACAGGAGAACTA+TGG + chr2_1:2998189-2998208 Msa0188030:intron 45.0%
GTGGTGAACAGGAGAACTAT+GGG + chr2_1:2998190-2998209 Msa0188030:intron 45.0%
!!! GCGTAGCGGATTTTTTGAGA+AGG + chr2_1:2998452-2998471 Msa0188030:intron 45.0%
AAGATGCAATAGGAGCTAGC+TGG + chr2_1:2998777-2998796 Msa0188030:three_prime_UTR 45.0%
AACAGTTGCAGGAACAGGAT+TGG + chr2_1:2999372-2999391 Msa0188030:three_prime_UTR 45.0%
CAGATTTACACAAGTGCAGC+AGG + chr2_1:2999446-2999465 Msa0188030:three_prime_UTR 45.0%
! CAACGTGGATGCGTCTTTTT+CGG + chr2_1:2999512-2999531 Msa0188030:three_prime_UTR 45.0%
ACCTATATCCGACACACACA+CGG - chr2_1:2999622-2999641 None:intergenic 45.0%
GTCGGATATAGGTGAAGCAT+TGG + chr2_1:2999629-2999648 Msa0188030:three_prime_UTR 45.0%
!! ATTTCGAGTTGGATGCCAAG+AGG + chr2_1:2999703-2999722 Msa0188030:three_prime_UTR 45.0%
!! TTTCGAGTTGGATGCCAAGA+GGG + chr2_1:2999704-2999723 Msa0188030:three_prime_UTR 45.0%
TGAACATCTTCTGCAGGCAC+CGG + chr2_1:2997106-2997125 Msa0188030:intron 50.0%
GAGGGTTCAATTCCTGGCAA+AGG - chr2_1:2997499-2997518 None:intergenic 50.0%
AAGGTCGAGGGTTCAATTCC+TGG - chr2_1:2997505-2997524 None:intergenic 50.0%
TGAGTGCACTGGATCATGCA+TGG + chr2_1:2997942-2997961 Msa0188030:intron 50.0%
ACTGCTTGAACTGCACTGCT+GGG - chr2_1:2998680-2998699 None:intergenic 50.0%
TACTGCTTGAACTGCACTGC+TGG - chr2_1:2998681-2998700 None:intergenic 50.0%
! CTGGAGTCCTGTTTTCTGTG+TGG + chr2_1:2998796-2998815 Msa0188030:three_prime_UTR 50.0%
GTCAGCTCCACACAGAAAAC+AGG - chr2_1:2998806-2998825 None:intergenic 50.0%
TAGCGAACAGTTGCAGGAAC+AGG + chr2_1:2999367-2999386 Msa0188030:three_prime_UTR 50.0%
! TTTGGTGGCAAAAACCGAGC+AGG + chr2_1:2999475-2999494 Msa0188030:three_prime_UTR 50.0%
! TTGGTGGCAAAAACCGAGCA+GGG + chr2_1:2999476-2999495 Msa0188030:three_prime_UTR 50.0%
TACTTAAGCCTTCCCCTGCT+CGG - chr2_1:2999492-2999511 None:intergenic 50.0%
GGGAAGGCTTAAGTACAACG+TGG + chr2_1:2999497-2999516 Msa0188030:three_prime_UTR 50.0%
CTATATCCGACACACACACG+GGG - chr2_1:2999620-2999639 None:intergenic 50.0%
CCTATATCCGACACACACAC+GGG - chr2_1:2999621-2999640 None:intergenic 50.0%
GACAACTCCCATACACGTCG+TGG + chr2_1:2996216-2996235 Msa0188030:CDS 55.0%
TGGCTGACCACGACGTGTAT+GGG - chr2_1:2996226-2996245 None:intergenic 55.0%
ATGGCTGACCACGACGTGTA+TGG - chr2_1:2996227-2996246 None:intergenic 55.0%
GCTGGGATGAGCTTCAATCG+AGG - chr2_1:2998663-2998682 None:intergenic 55.0%
! GTTGCAGGAACAGGATTGGC+AGG + chr2_1:2999376-2999395 Msa0188030:three_prime_UTR 55.0%
TACACAAGTGCAGCAGGGTC+AGG + chr2_1:2999452-2999471 Msa0188030:three_prime_UTR 55.0%
AAGTGCAGCAGGGTCAGGTT+TGG + chr2_1:2999457-2999476 Msa0188030:three_prime_UTR 55.0%
TGGTGGCAAAAACCGAGCAG+GGG + chr2_1:2999477-2999496 Msa0188030:three_prime_UTR 55.0%
CCCGTGTGTGTGTCGGATAT+AGG + chr2_1:2999618-2999637 Msa0188030:three_prime_UTR 55.0%
CACGTCGTGGTCAGCCATGT+TGG + chr2_1:2996229-2996248 Msa0188030:CDS 60.0%
TGCAGCAGGGTCAGGTTTGG+TGG + chr2_1:2999460-2999479 Msa0188030:three_prime_UTR 60.0%
GGCAAAAACCGAGCAGGGGA+AGG + chr2_1:2999481-2999500 Msa0188030:three_prime_UTR 60.0%
GTCAAGCCCCGTGTGTGTGT+CGG + chr2_1:2999611-2999630 Msa0188030:three_prime_UTR 60.0%
TCCGCTACGCGCCGCGATAG+CGG - chr2_1:2998442-2998461 None:intergenic 70.0%
!! CTACGCGCCGCGATAGCGGG+CGG - chr2_1:2998438-2998457 None:intergenic 75.0%
CCGCTACGCGCCGCGATAGC+GGG - chr2_1:2998441-2998460 None:intergenic 75.0%
CCCGCTATCGCGGCGCGTAG+CGG + chr2_1:2998438-2998457 Msa0188030:intron 75.0%
! GGCTGCGCCGCCCGCTATCG+CGG + chr2_1:2998428-2998447 Msa0188030:intron 80.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 2996133 2999778 2996133 ID=Msa0188030;Name=Msa0188030
chr2_1 mRNA 2996133 2999778 2996133 ID=Msa0188030-mRNA-1;Parent=Msa0188030;Name=Msa0188030-mRNA-1;_AED=0.04;_eAED=0.04;_QI=80|1|1|1|1|1|9|1036|247
chr2_1 exon 2996133 2996266 2996133 ID=Msa0188030-mRNA-1:exon:1309;Parent=Msa0188030-mRNA-1
chr2_1 exon 2996356 2996472 2996356 ID=Msa0188030-mRNA-1:exon:1310;Parent=Msa0188030-mRNA-1
chr2_1 exon 2996554 2996642 2996554 ID=Msa0188030-mRNA-1:exon:1311;Parent=Msa0188030-mRNA-1
chr2_1 exon 2997122 2997173 2997122 ID=Msa0188030-mRNA-1:exon:1312;Parent=Msa0188030-mRNA-1
chr2_1 exon 2997669 2997764 2997669 ID=Msa0188030-mRNA-1:exon:1313;Parent=Msa0188030-mRNA-1
chr2_1 exon 2997896 2997940 2997896 ID=Msa0188030-mRNA-1:exon:1314;Parent=Msa0188030-mRNA-1
chr2_1 exon 2998020 2998109 2998020 ID=Msa0188030-mRNA-1:exon:1315;Parent=Msa0188030-mRNA-1
chr2_1 exon 2998201 2998272 2998201 ID=Msa0188030-mRNA-1:exon:1316;Parent=Msa0188030-mRNA-1
chr2_1 exon 2998614 2999778 2998614 ID=Msa0188030-mRNA-1:exon:1317;Parent=Msa0188030-mRNA-1
chr2_1 five_prime_UTR 2996133 2996212 2996133 ID=Msa0188030-mRNA-1:five_prime_utr;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2996213 2996266 2996213 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2996356 2996472 2996356 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2996554 2996642 2996554 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2997122 2997173 2997122 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2997669 2997764 2997669 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2997896 2997940 2997896 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2998020 2998109 2998020 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2998201 2998272 2998201 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 CDS 2998614 2998742 2998614 ID=Msa0188030-mRNA-1:cds;Parent=Msa0188030-mRNA-1
chr2_1 three_prime_UTR 2998743 2999778 2998743 ID=Msa0188030-mRNA-1:three_prime_utr;Parent=Msa0188030-mRNA-1
Gene Sequence

>Msa0188030

ATGGACAACTCCCATACACGTCGTGGTCAGCCATGTTGGTTTAGAGTACCCAAGGTTGGAATGATTGCTGCGAATGATGGAGTTCTACTAAGAAACCATATTCCGCGTATCCTTAGGAAACACTTCAAGGGAAAGCCGTATTATGTTGAACTTCTTGATTTATTCAATGAGGTTGAGTTTCAAACTGCTGCTGGACAGATGATCGATTTGATCACCACACTGGAAGGAGAAAAAGACCTGTCCAAATACACCTTATCACTGCACCGGCGTATTGTTCAGTACAAGACTGCCTATTATTCTTTTTACCTTCCTGTTGCATGTGCATTACTAATGGCTGGTGAAGATCTGGATAACCATGTTGATGTAAAGAACATTCTTGTTGAGATGGGAACATATTTTCAAGTTCAGGATGATTATTTGGATTGCTTTGGTGATCCTGAAACAATTGGAAAGATAGGCACAGATATTGAAGACTTTAAGTGCTCTTGGGTGGTTGTGAAAGCATTGGAACTTTGCAATGAAGAACAAAAGAAGGTTTTACATGAGAACTATGGGAAGCCAGATCCTGCAAATGTTGCTATAGTCAAGACCCTTTATAACGAGCTTAATCTCGAGGGTGCATTTGCGGAGTATGAGAGTGCGAGCTATGAGAAGCTTGTAACCTCGATTGAAGCTCATCCCAGCAGTGCAGTTCAAGCAGTATTGAAGTCCTTCTTGGCTAAAATTTACAAGAGGCAGAAGTAG
Protein sequence

>Msa0188030

MDNSHTRRGQPCWFRVPKVGMIAANDGVLLRNHIPRILRKHFKGKPYYVELLDLFNEVEFQTAAGQMIDLITTLEGEKDLSKYTLSLHRRIVQYKTAYYSFYLPVACALLMAGEDLDNHVDVKNILVEMGTYFQVQDDYLDCFGDPETIGKIGTDIEDFKCSWVVVKALELCNEEQKKVLHENYGKPDPANVAIVKTLYNELNLEGAFAEYESASYEKLVTSIEAHPSSAVQAVLKSFLAKIYKRQK*