AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192540 MtrunA17_Chr2g0282691 93.962 265 14 2 1 265 1 263 9.07e-110 317
Msa0192540 MtrunA17_Chr4g0046601 56.618 136 47 5 131 262 230 357 5.68e-22 93.6
Msa0192540 MtrunA17_Chr4g0046601 41.791 134 75 2 1 132 1 133 1.62e-18 84.0
Msa0192540 MtrunA17_Chr2g0324881 30.859 256 117 8 21 255 21 237 5.57e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTGGTTTGTTTGTAAC+TGG 0.131657 2_1:-8349999 None:intergenic
TGTAGTTGCCATGGTTATTC+TGG 0.221088 2_1:-8350568 None:intergenic
TTGTGTCACTCAACTCATTT+TGG 0.248728 2_1:-8350226 None:intergenic
CAAAATAGCTATGGCTTATA+TGG 0.260837 2_1:+8350047 Msa0192540:CDS
TAGTACTTGTTATTGTTGTT+GGG 0.319644 2_1:-8350149 None:intergenic
GTAGTACTTGTTATTGTTGT+TGG 0.324905 2_1:-8350150 None:intergenic
TATTACAACAAAGACAGTTT+TGG 0.329701 2_1:+8350197 Msa0192540:CDS
AACTGGGGATTCTTCTTTGT+TGG 0.334551 2_1:-8349982 None:intergenic
ATGAAAGGTGGTTGCTCCTC+TGG 0.339057 2_1:-8350014 None:intergenic
ATTATAATGCTCAGAATAAC+AGG 0.351179 2_1:+8350369 Msa0192540:CDS
GGTGGATGCCGTGGCGGCGG+TGG 0.353742 2_1:-8350093 None:intergenic
ACTGGGGATTCTTCTTTGTT+GGG 0.370013 2_1:-8349981 None:intergenic
CCTCTGGTTTGTTTGTAACT+GGG 0.383487 2_1:-8349998 None:intergenic
TACAACAATAATAATAACTA+TGG 0.387734 2_1:+8350530 Msa0192540:CDS
GATGAATCACCATACATTGT+TGG 0.414049 2_1:-8350479 None:intergenic
TGTTTCTTCAAAGTTAGAAT+TGG 0.416528 2_1:-8350117 None:intergenic
GCTGAAGAATTCACTCTCTC+TGG 0.421980 2_1:-8349925 None:intergenic
GAGTGAACAACAATAATAAC+TGG 0.430385 2_1:+8350507 Msa0192540:CDS
TTCTTCAAAGTTAGAATTGG+TGG 0.439756 2_1:-8350114 None:intergenic
AAATACAATCCAACAATGTA+TGG 0.452288 2_1:+8350470 Msa0192540:CDS
TAAGAGGATGAAAGAAATGT+AGG 0.456040 2_1:-8349871 None:intergenic
GATGCCGTGGCGGCGGTGGA+AGG 0.457928 2_1:-8350089 None:intergenic
GGTGGTGGATGCCGTGGCGG+CGG 0.460270 2_1:-8350096 None:intergenic
CTCTGGTTTGTTTGTAACTG+GGG 0.464914 2_1:-8349997 None:intergenic
AATTATTATAATTATTGTTG+TGG 0.469980 2_1:-8350349 None:intergenic
TGTTGTTGAAGTAGTACTCT+TGG 0.484835 2_1:-8350298 None:intergenic
GCAACAAAAGAGTTGTTAAG+AGG 0.489505 2_1:-8349887 None:intergenic
AGGATGAAAGAAATGTAGGT+TGG 0.497294 2_1:-8349867 None:intergenic
GGATGAAAGAAATGTAGGTT+GGG 0.498004 2_1:-8349866 None:intergenic
TGTAACCATTGTAGTTGCCA+TGG 0.515915 2_1:-8350577 None:intergenic
TTCAAAGTTAGAATTGGTGG+TGG 0.520386 2_1:-8350111 None:intergenic
AGTACTTGTTATTGTTGTTG+GGG 0.535885 2_1:-8350148 None:intergenic
GACACAAAGTACAATGAAGA+AGG 0.560331 2_1:+8350242 Msa0192540:CDS
CCTCAGACACAAAATAGCTA+TGG 0.562030 2_1:+8350038 Msa0192540:CDS
CATTCCTTCCACCGCCGCCA+CGG 0.564976 2_1:+8350085 Msa0192540:CDS
AGAAGGTTACAACTCCATGA+TGG 0.580265 2_1:+8350259 Msa0192540:CDS
AGGTACAATGGTGAGAAACA+AGG 0.586625 2_1:+8350389 Msa0192540:CDS
ATGTATGGTGATTCATCTAG+AGG 0.598645 2_1:+8350485 Msa0192540:CDS
TGTGTCTGAGGAATGAAAGG+TGG 0.603943 2_1:-8350026 None:intergenic
AATAACCATGGCAACTACAA+TGG 0.610902 2_1:+8350572 Msa0192540:CDS
GCTCAGAATAACAGGTACAA+TGG 0.619912 2_1:+8350377 Msa0192540:CDS
AAGGAATGTGATCAGAGTCA+TGG 0.626750 2_1:-8350070 None:intergenic
AGAATTGGTGGTGGATGCCG+TGG 0.633457 2_1:-8350102 None:intergenic
ATTGGTGGTGGATGCCGTGG+CGG 0.636866 2_1:-8350099 None:intergenic
GGTACAATGGTGAGAAACAA+GGG 0.647160 2_1:+8350390 Msa0192540:CDS
AGAAATGTAGGTTGGGAATG+TGG 0.649922 2_1:-8349859 None:intergenic
TCTCTGGCTATAGTTTGTAG+AGG 0.668021 2_1:-8349909 None:intergenic
AATGAGTACCAGAATAACCA+TGG 0.671041 2_1:+8350560 Msa0192540:CDS
CCCAGTTACAAACAAACCAG+AGG 0.679700 2_1:+8349998 Msa0192540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTATAATTATTGTTG+TGG - chr2_1:8350352-8350371 None:intergenic 10.0%
!! AATTATTATAATTATTGTTG+TGG - chr2_1:8350352-8350371 None:intergenic 10.0%
!! TACAACAATAATAATAACTA+TGG + chr2_1:8350530-8350549 Msa0192540:CDS 15.0%
!! TACAACAATAATAATAACTA+TGG + chr2_1:8350530-8350549 Msa0192540:CDS 15.0%
! TGTTTCTTCAAAGTTAGAAT+TGG - chr2_1:8350120-8350139 None:intergenic 25.0%
!!! TAGTACTTGTTATTGTTGTT+GGG - chr2_1:8350152-8350171 None:intergenic 25.0%
!! TATTACAACAAAGACAGTTT+TGG + chr2_1:8350197-8350216 Msa0192540:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG + chr2_1:8350369-8350388 Msa0192540:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG + chr2_1:8350470-8350489 Msa0192540:CDS 25.0%
! TGTTTCTTCAAAGTTAGAAT+TGG - chr2_1:8350120-8350139 None:intergenic 25.0%
!!! TAGTACTTGTTATTGTTGTT+GGG - chr2_1:8350152-8350171 None:intergenic 25.0%
!! TATTACAACAAAGACAGTTT+TGG + chr2_1:8350197-8350216 Msa0192540:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG + chr2_1:8350369-8350388 Msa0192540:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG + chr2_1:8350470-8350489 Msa0192540:CDS 25.0%
TAAGAGGATGAAAGAAATGT+AGG - chr2_1:8349874-8349893 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG + chr2_1:8350047-8350066 Msa0192540:CDS 30.0%
TTCTTCAAAGTTAGAATTGG+TGG - chr2_1:8350117-8350136 None:intergenic 30.0%
!! AGTACTTGTTATTGTTGTTG+GGG - chr2_1:8350151-8350170 None:intergenic 30.0%
!! GTAGTACTTGTTATTGTTGT+TGG - chr2_1:8350153-8350172 None:intergenic 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG - chr2_1:8350276-8350295 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG + chr2_1:8350507-8350526 Msa0192540:CDS 30.0%
TAAGAGGATGAAAGAAATGT+AGG - chr2_1:8349874-8349893 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG + chr2_1:8350047-8350066 Msa0192540:CDS 30.0%
TTCTTCAAAGTTAGAATTGG+TGG - chr2_1:8350117-8350136 None:intergenic 30.0%
!! AGTACTTGTTATTGTTGTTG+GGG - chr2_1:8350151-8350170 None:intergenic 30.0%
!! GTAGTACTTGTTATTGTTGT+TGG - chr2_1:8350153-8350172 None:intergenic 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG - chr2_1:8350276-8350295 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG + chr2_1:8350507-8350526 Msa0192540:CDS 30.0%
GGATGAAAGAAATGTAGGTT+GGG - chr2_1:8349869-8349888 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG - chr2_1:8349870-8349889 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG - chr2_1:8349890-8349909 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG - chr2_1:8350032-8350051 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG - chr2_1:8350114-8350133 None:intergenic 35.0%
!! TTGTGTCACTCAACTCATTT+TGG - chr2_1:8350229-8350248 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG + chr2_1:8350242-8350261 Msa0192540:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG - chr2_1:8350301-8350320 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG + chr2_1:8350412-8350431 Msa0192540:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG + chr2_1:8350416-8350435 Msa0192540:CDS 35.0%
GATGAATCACCATACATTGT+TGG - chr2_1:8350482-8350501 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG + chr2_1:8350485-8350504 Msa0192540:CDS 35.0%
AATGAGTACCAGAATAACCA+TGG + chr2_1:8350560-8350579 Msa0192540:CDS 35.0%
AATAACCATGGCAACTACAA+TGG + chr2_1:8350572-8350591 Msa0192540:CDS 35.0%
CAATGGTTACAAAAACCAAG+AGG + chr2_1:8350589-8350608 Msa0192540:CDS 35.0%
TTACAAAAACCAAGAGGAGT+TGG + chr2_1:8350595-8350614 Msa0192540:CDS 35.0%
GGATGAAAGAAATGTAGGTT+GGG - chr2_1:8349869-8349888 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG - chr2_1:8349870-8349889 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG - chr2_1:8349890-8349909 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG - chr2_1:8350032-8350051 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG - chr2_1:8350114-8350133 None:intergenic 35.0%
!! TTGTGTCACTCAACTCATTT+TGG - chr2_1:8350229-8350248 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG + chr2_1:8350242-8350261 Msa0192540:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG - chr2_1:8350301-8350320 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG + chr2_1:8350412-8350431 Msa0192540:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG + chr2_1:8350416-8350435 Msa0192540:CDS 35.0%
GATGAATCACCATACATTGT+TGG - chr2_1:8350482-8350501 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG + chr2_1:8350485-8350504 Msa0192540:CDS 35.0%
AATGAGTACCAGAATAACCA+TGG + chr2_1:8350560-8350579 Msa0192540:CDS 35.0%
AATAACCATGGCAACTACAA+TGG + chr2_1:8350572-8350591 Msa0192540:CDS 35.0%
CAATGGTTACAAAAACCAAG+AGG + chr2_1:8350589-8350608 Msa0192540:CDS 35.0%
TTACAAAAACCAAGAGGAGT+TGG + chr2_1:8350595-8350614 Msa0192540:CDS 35.0%
! AGAAATGTAGGTTGGGAATG+TGG - chr2_1:8349862-8349881 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG - chr2_1:8349912-8349931 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG - chr2_1:8349984-8350003 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG - chr2_1:8349985-8350004 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG - chr2_1:8350000-8350019 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG - chr2_1:8350001-8350020 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG - chr2_1:8350002-8350021 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG - chr2_1:8350041-8350060 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG + chr2_1:8350038-8350057 Msa0192540:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG - chr2_1:8350073-8350092 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG + chr2_1:8350259-8350278 Msa0192540:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG + chr2_1:8350377-8350396 Msa0192540:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG + chr2_1:8350389-8350408 Msa0192540:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG + chr2_1:8350390-8350409 Msa0192540:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG + chr2_1:8350419-8350438 Msa0192540:CDS 40.0%
TGTAGTTGCCATGGTTATTC+TGG - chr2_1:8350571-8350590 None:intergenic 40.0%
TGTAACCATTGTAGTTGCCA+TGG - chr2_1:8350580-8350599 None:intergenic 40.0%
! GTTCATTTTCCAACTCCTCT+TGG - chr2_1:8350607-8350626 None:intergenic 40.0%
! AGAAATGTAGGTTGGGAATG+TGG - chr2_1:8349862-8349881 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG - chr2_1:8349912-8349931 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG - chr2_1:8349984-8350003 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG - chr2_1:8349985-8350004 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG - chr2_1:8350000-8350019 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG - chr2_1:8350001-8350020 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG - chr2_1:8350002-8350021 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG - chr2_1:8350041-8350060 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG + chr2_1:8350038-8350057 Msa0192540:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG - chr2_1:8350073-8350092 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG + chr2_1:8350259-8350278 Msa0192540:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG + chr2_1:8350377-8350396 Msa0192540:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG + chr2_1:8350389-8350408 Msa0192540:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG + chr2_1:8350390-8350409 Msa0192540:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG + chr2_1:8350419-8350438 Msa0192540:CDS 40.0%
TGTAGTTGCCATGGTTATTC+TGG - chr2_1:8350571-8350590 None:intergenic 40.0%
TGTAACCATTGTAGTTGCCA+TGG - chr2_1:8350580-8350599 None:intergenic 40.0%
! GTTCATTTTCCAACTCCTCT+TGG - chr2_1:8350607-8350626 None:intergenic 40.0%
GCTGAAGAATTCACTCTCTC+TGG - chr2_1:8349928-8349947 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG + chr2_1:8349998-8350017 Msa0192540:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG - chr2_1:8350029-8350048 None:intergenic 45.0%
GCTGAAGAATTCACTCTCTC+TGG - chr2_1:8349928-8349947 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG + chr2_1:8349998-8350017 Msa0192540:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG - chr2_1:8350029-8350048 None:intergenic 45.0%
ATGAAAGGTGGTTGCTCCTC+TGG - chr2_1:8350017-8350036 None:intergenic 50.0%
ATGAAAGGTGGTTGCTCCTC+TGG - chr2_1:8350017-8350036 None:intergenic 50.0%
!! AGAATTGGTGGTGGATGCCG+TGG - chr2_1:8350105-8350124 None:intergenic 55.0%
!! AGAATTGGTGGTGGATGCCG+TGG - chr2_1:8350105-8350124 None:intergenic 55.0%
!! ATTGGTGGTGGATGCCGTGG+CGG - chr2_1:8350102-8350121 None:intergenic 60.0%
!! ATTGGTGGTGGATGCCGTGG+CGG - chr2_1:8350102-8350121 None:intergenic 60.0%
CATTCCTTCCACCGCCGCCA+CGG + chr2_1:8350085-8350104 Msa0192540:CDS 65.0%
CATTCCTTCCACCGCCGCCA+CGG + chr2_1:8350085-8350104 Msa0192540:CDS 65.0%
GATGCCGTGGCGGCGGTGGA+AGG - chr2_1:8350092-8350111 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG - chr2_1:8350099-8350118 None:intergenic 75.0%
GATGCCGTGGCGGCGGTGGA+AGG - chr2_1:8350092-8350111 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG - chr2_1:8350099-8350118 None:intergenic 75.0%
! GGTGGATGCCGTGGCGGCGG+TGG - chr2_1:8350096-8350115 None:intergenic 80.0%
! GGTGGATGCCGTGGCGGCGG+TGG - chr2_1:8350096-8350115 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8349855 8350652 8349855 ID=Msa0192540;Name=Msa0192540
chr2_1 mRNA 8349855 8350652 8349855 ID=Msa0192540-mRNA-1;Parent=Msa0192540;Name=Msa0192540-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|265
chr2_1 exon 8349855 8350652 8349855 ID=Msa0192540-mRNA-1:exon:3818;Parent=Msa0192540-mRNA-1
chr2_1 CDS 8349855 8350652 8349855 ID=Msa0192540-mRNA-1:cds;Parent=Msa0192540-mRNA-1
Gene Sequence

>Msa0192540

ATGGCCACATTCCCAACCTACATTTCTTTCATCCTCTTAACAACTCTTTTGTTGCCTCTACAAACTATAGCCAGAGAGAGTGAATTCTTCAGCAAAGTCACACATTTCAACAAAGATACAAAAGTTCCCAACAAAGAAGAATCCCCAGTTACAAACAAACCAGAGGAGCAACCACCTTTCATTCCTCAGACACAAAATAGCTATGGCTTATATGGCCATGACTCTGATCACATTCCTTCCACCGCCGCCACGGCATCCACCACCAATTCTAACTTTGAAGAAACAAGCAATTACCCCAACAACAATAACAAGTACTACAACAATGATGCATATAACACAAAGTATTACAACAAAGACAGTTTTGGAAATAACCAAAATGAGTTGAGTGACACAAAGTACAATGAAGAAGGTTACAACTCCATGATGGAAAAACAAAACAACAACCAAGAGTACTACTTCAACAACAACAACAATGCTGCAAATGAAAGATCATTCCACAACAATAATTATAATAATTATAATGCTCAGAATAACAGGTACAATGGTGAGAAACAAGGGATGAGTGACACAAGATTTTTGGAAGGTGGAAAATATTTCTATGATATTAACTATGAGAAATACAATCCAACAATGTATGGTGATTCATCTAGAGGAGTGAACAACAATAATAACTGGTACAACAATAATAATAACTATGGTAACAACAATGAGTACCAGAATAACCATGGCAACTACAATGGTTACAAAAACCAAGAGGAGTTGGAAAATGAACAACATGAGTTTGATGTTTTACCATAA

Protein sequence

>Msa0192540

MATFPTYISFILLTTLLLPLQTIARESEFFSKVTHFNKDTKVPNKEESPVTNKPEEQPPFIPQTQNSYGLYGHDSDHIPSTAATASTTNSNFEETSNYPNNNNKYYNNDAYNTKYYNKDSFGNNQNELSDTKYNEEGYNSMMEKQNNNQEYYFNNNNNAANERSFHNNNYNNYNAQNNRYNGEKQGMSDTRFLEGGKYFYDINYEKYNPTMYGDSSRGVNNNNNWYNNNNNYGNNNEYQNNHGNYNGYKNQEELENEQHEFDVLP*