AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0214090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAAGAATTTCAAGATTT+TGG 0.081409 2_1:+58265594 Msa0214090:three_prime_UTR
ATATATTATTGGCAAGTATT+TGG 0.218945 2_1:+58265761 Msa0214090:three_prime_UTR
TAAAGAATGTATATTATAAT+TGG 0.226983 2_1:-58262478 None:intergenic
GTATCTGAATGATATATTAT+TGG 0.297738 2_1:+58265750 Msa0214090:three_prime_UTR
ACTTTATTGTTGTGTCTCTT+TGG 0.303592 2_1:+58265465 Msa0214090:three_prime_UTR
TAAATATTCGAGTACTTTAT+CGG 0.305611 2_1:+58265705 Msa0214090:three_prime_UTR
ATCTCCACAGTTGCATAGTA+TGG 0.312196 2_1:+58265207 Msa0214090:CDS
TAATAGGACTTTGTTTCCTT+TGG 0.320333 2_1:+58262710 Msa0214090:five_prime_UTR
TAGGACTTTGTTTCCTTTGG+TGG 0.342352 2_1:+58262713 Msa0214090:five_prime_UTR
TCAAGTATAAGAAGGGAAAA+AGG 0.388627 2_1:+58262454 None:intergenic
AGAAGTACGAGTTCTGAAAA+AGG 0.395320 2_1:+58265109 Msa0214090:five_prime_UTR
TATTGTTGTGTCTCTTTGGA+AGG 0.397116 2_1:+58265469 Msa0214090:three_prime_UTR
GGACAATATAGTTATGTTGA+TGG 0.429763 2_1:+58265231 Msa0214090:CDS
ATATTCTATGTAGGTGGCCT+AGG 0.434136 2_1:+58265361 Msa0214090:three_prime_UTR
AAACTCAAATTAAAAGGGAT+TGG 0.439201 2_1:-58262628 None:intergenic
AGTATTATCATATGAAAAGA+AGG 0.444549 2_1:+58265409 Msa0214090:three_prime_UTR
TAAACAAAACACACCACAAC+AGG 0.445668 2_1:-58262741 None:intergenic
AGAGTGGTGAAGTTAGAGTA+AGG 0.456117 2_1:-58263492 None:intergenic
AGTTATGTTGATGGTTCAGC+AGG 0.481403 2_1:+58265240 Msa0214090:CDS
GCAACAACAAATGTCCTGAT+TGG 0.487793 2_1:-58263516 None:intergenic
ATTGTTGTGTCTCTTTGGAA+GGG 0.494366 2_1:+58265470 Msa0214090:three_prime_UTR
TGTGTCTCTTTGGAAGGGAG+TGG 0.495873 2_1:+58265475 Msa0214090:three_prime_UTR
ACTTCACCACTCTACCAATC+AGG 0.496727 2_1:+58263502 Msa0214090:five_prime_UTR
AAATTAAAAGGGATTGGATC+AGG 0.497048 2_1:-58262622 None:intergenic
AGTAGAGAAATATTCTATGT+AGG 0.502287 2_1:+58265352 Msa0214090:three_prime_UTR
TTACTAGTAGAAGTACTCAT+TGG 0.525694 2_1:-58265147 None:intergenic
AGGTCCTCAATCAAGATCCT+AGG 0.526371 2_1:-58265378 None:intergenic
TATGATAATACTAAACTAGT+AGG 0.528194 2_1:-58265398 None:intergenic
TTTGGTGGATGTACCTGTTG+TGG 0.552797 2_1:+58262728 Msa0214090:five_prime_UTR
GATGCCACTCATGGTTAGAA+AGG 0.560239 2_1:-58262564 None:intergenic
TTAAAACGCAGAACAGCTGC+AGG 0.564636 2_1:-58262771 None:intergenic
GTGGCCTAGGATCTTGATTG+AGG 0.582897 2_1:+58265374 Msa0214090:three_prime_UTR
AAATTTAATAACGTGTGTTG+TGG 0.588206 2_1:+58265289 Msa0214090:three_prime_UTR
CTATCCTTTCTAACCATGAG+TGG 0.598762 2_1:+58262560 Msa0214090:five_prime_UTR
AGTGAGTACTCTTCAACCAA+AGG 0.627082 2_1:+58263542 Msa0214090:five_prime_UTR
AGAGAAATATTCTATGTAGG+TGG 0.633840 2_1:+58265355 Msa0214090:three_prime_UTR
AAATGTCCTGATTGGTAGAG+TGG 0.637316 2_1:-58263508 None:intergenic
TCCACAGTTGCATAGTATGG+AGG 0.654780 2_1:+58265210 Msa0214090:CDS
TCCTCCATACTATGCAACTG+TGG 0.658216 2_1:-58265211 None:intergenic
ACAACAGGTACATCCACCAA+AGG 0.675199 2_1:-58262726 None:intergenic
ATTGTATATGTCAAACACTG+AGG 0.705615 2_1:-58265512 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGAATGTATATTATAAT+TGG - chr2_1:58262481-58262500 None:intergenic 10.0%
!! TATGTATCTAATAATATTAT+AGG + chr2_1:58263439-58263458 Msa0214090:intron 10.0%
!!! TAATTTTATTTTTTATTAGC+TGG + chr2_1:58263794-58263813 Msa0214090:intron 10.0%
!! TTTGTTTATTTGTATTGTAT+TGG + chr2_1:58264432-58264451 Msa0214090:intron 15.0%
!! TTGTTTATTTGTATTGTATT+GGG + chr2_1:58264433-58264452 Msa0214090:intron 15.0%
!! GTCAAAAACTCAAATTAAAA+GGG - chr2_1:58262636-58262655 None:intergenic 20.0%
!! TGTCAAAAACTCAAATTAAA+AGG - chr2_1:58262637-58262656 None:intergenic 20.0%
!! AAGAAGAATATCAAACAAAA+CGG + chr2_1:58263003-58263022 Msa0214090:intron 20.0%
!! TAATAATGTAAAAACCGAAA+AGG - chr2_1:58263224-58263243 None:intergenic 20.0%
!! AATTAACCACTAAAGTAATT+AGG - chr2_1:58263251-58263270 None:intergenic 20.0%
!! TCTAATAATATTATAGGTTG+CGG + chr2_1:58263445-58263464 Msa0214090:intron 20.0%
!!! ATTTTTTATTAGCTGGTAAA+TGG + chr2_1:58263801-58263820 Msa0214090:intron 20.0%
!!! TTTTTTATTAGCTGGTAAAT+GGG + chr2_1:58263802-58263821 Msa0214090:intron 20.0%
!! TACAATAAACAATGATAAAC+AGG - chr2_1:58264021-58264040 None:intergenic 20.0%
!! ACTATTAATGAGTAGATATA+AGG - chr2_1:58264178-58264197 None:intergenic 20.0%
!! TACAAATAAACAAAAAGAGA+AGG - chr2_1:58264426-58264445 None:intergenic 20.0%
!! TTTGTATTGTTATAAACCAT+AGG - chr2_1:58264755-58264774 None:intergenic 20.0%
!!! AGTTTCAATTACTTTGTTAT+AGG + chr2_1:58264799-58264818 Msa0214090:intron 20.0%
!!! ACTAAGTTTTTTTTTTCTCT+GGG + chr2_1:58265049-58265068 Msa0214090:intron 20.0%
!!! AATTTTCAAGATGGTTTTAT+TGG + chr2_1:58265168-58265187 Msa0214090:exon 20.0%
!! TATGATAATACTAAACTAGT+AGG - chr2_1:58265401-58265420 None:intergenic 20.0%
!! AGTATTATCATATGAAAAGA+AGG + chr2_1:58265409-58265428 Msa0214090:three_prime_UTR 20.0%
!!! AATGAAGAATTTCAAGATTT+TGG + chr2_1:58265594-58265613 Msa0214090:three_prime_UTR 20.0%
!! TAAATATTCGAGTACTTTAT+CGG + chr2_1:58265705-58265724 Msa0214090:three_prime_UTR 20.0%
!! GTATCTGAATGATATATTAT+TGG + chr2_1:58265750-58265769 Msa0214090:three_prime_UTR 20.0%
!! ATATATTATTGGCAAGTATT+TGG + chr2_1:58265761-58265780 Msa0214090:three_prime_UTR 20.0%
!!! TGGTATGTAATTTTGAATTT+AGG + chr2_1:58265781-58265800 Msa0214090:intron 20.0%
! AAACTCAAATTAAAAGGGAT+TGG - chr2_1:58262631-58262650 None:intergenic 25.0%
!! ATCTGAAGGTATAAAAATGA+GGG - chr2_1:58262679-58262698 None:intergenic 25.0%
! TTACTAAAAAAGTGATCTGA+AGG - chr2_1:58262693-58262712 None:intergenic 25.0%
!!! CAGATCACTTTTTTAGTAAT+AGG + chr2_1:58262694-58262713 Msa0214090:five_prime_UTR 25.0%
!!! TTTAATATGGGTATTGGTTT+TGG + chr2_1:58262789-58262808 Msa0214090:intron 25.0%
! AATTCAATAGGAACTTTATG+AGG - chr2_1:58263047-58263066 None:intergenic 25.0%
! CATAAAGTTCCTATTGAATT+TGG + chr2_1:58263047-58263066 Msa0214090:intron 25.0%
!!! TTTTTTTCCCATTTTCCTTT+TGG + chr2_1:58263134-58263153 Msa0214090:intron 25.0%
! ATTAAATAATGTCTGCATGT+GGG + chr2_1:58263346-58263365 Msa0214090:intron 25.0%
! ATTTCAATTGTATCAATGCA+TGG - chr2_1:58263594-58263613 None:intergenic 25.0%
! ATACACTGCAAAAGAAATAA+CGG + chr2_1:58263619-58263638 Msa0214090:intron 25.0%
!!! TCTTTGAGAAGGTATTTATA+TGG + chr2_1:58263760-58263779 Msa0214090:intron 25.0%
!! TTTTTATTAGCTGGTAAATG+GGG + chr2_1:58263803-58263822 Msa0214090:intron 25.0%
! AAACAAATCAAACCATTCTT+TGG - chr2_1:58263848-58263867 None:intergenic 25.0%
! TACAAGAGGAATTAGAAATA+TGG - chr2_1:58264072-58264091 None:intergenic 25.0%
! ACTATTAACTATCTTACAAG+AGG - chr2_1:58264086-58264105 None:intergenic 25.0%
!! AAACTTTAGAAAATCACTCT+AGG + chr2_1:58264773-58264792 Msa0214090:intron 25.0%
! GCTTAATCACTTAATATTAG+TGG + chr2_1:58264910-58264929 Msa0214090:intron 25.0%
! TGTAGATCAACAAAACTATA+TGG + chr2_1:58265001-58265020 Msa0214090:intron 25.0%
!!! GACTAAGTTTTTTTTTTCTC+TGG + chr2_1:58265048-58265067 Msa0214090:intron 25.0%
!!! TCTACTAGTAATTTTCAAGA+TGG + chr2_1:58265159-58265178 Msa0214090:exon 25.0%
!!! TGGTTTTATTGGTAGTTATA+AGG + chr2_1:58265179-58265198 Msa0214090:CDS 25.0%
! AAATTTAATAACGTGTGTTG+TGG + chr2_1:58265289-58265308 Msa0214090:three_prime_UTR 25.0%
! AGTAGAGAAATATTCTATGT+AGG + chr2_1:58265352-58265371 Msa0214090:three_prime_UTR 25.0%
AAATTAAAAGGGATTGGATC+AGG - chr2_1:58262625-58262644 None:intergenic 30.0%
! GATCTGAAGGTATAAAAATG+AGG - chr2_1:58262680-58262699 None:intergenic 30.0%
! TAATAGGACTTTGTTTCCTT+TGG + chr2_1:58262710-58262729 Msa0214090:five_prime_UTR 30.0%
!!! TTGTGGTGTGTTTTGTTTAT+TGG + chr2_1:58262745-58262764 Msa0214090:five_prime_UTR 30.0%
AAATCTAGCAGCTAATACAA+AGG - chr2_1:58262840-58262859 None:intergenic 30.0%
AGAAGAAATACAAAGCAAAC+GGG - chr2_1:58262934-58262953 None:intergenic 30.0%
CAGAAGAAATACAAAGCAAA+CGG - chr2_1:58262935-58262954 None:intergenic 30.0%
ATAAAACAGAATCAGCCAAA+AGG - chr2_1:58263152-58263171 None:intergenic 30.0%
!!! AACTAGCTTTGATTCCTTTT+CGG + chr2_1:58263207-58263226 Msa0214090:intron 30.0%
TAACCACCTAATTACTTTAG+TGG + chr2_1:58263242-58263261 Msa0214090:intron 30.0%
TAACCACTAAAGTAATTAGG+TGG - chr2_1:58263248-58263267 None:intergenic 30.0%
AATCTTGAAGAATAGATGTG+GGG - chr2_1:58263312-58263331 None:intergenic 30.0%
CAATCTTGAAGAATAGATGT+GGG - chr2_1:58263313-58263332 None:intergenic 30.0%
ACAATCTTGAAGAATAGATG+TGG - chr2_1:58263314-58263333 None:intergenic 30.0%
CATTAAATAATGTCTGCATG+TGG + chr2_1:58263345-58263364 Msa0214090:intron 30.0%
TAGTTTCAATCTTCTCACTT+TGG - chr2_1:58263674-58263693 None:intergenic 30.0%
! TTTTATTAGCTGGTAAATGG+GGG + chr2_1:58263804-58263823 Msa0214090:intron 30.0%
! TTTTTGTTGCATCCAAAGAA+TGG + chr2_1:58263833-58263852 Msa0214090:intron 30.0%
!! AGAATGGTTTGATTTGTTTG+AGG + chr2_1:58263849-58263868 Msa0214090:intron 30.0%
AATGAGTAGTGTTAAATCAG+TGG - chr2_1:58263897-58263916 None:intergenic 30.0%
TTCCCTTAACTTATGAGTAT+AGG + chr2_1:58263954-58263973 Msa0214090:intron 30.0%
CTCCTATACTCATAAGTTAA+GGG - chr2_1:58263959-58263978 None:intergenic 30.0%
GACAAGAGTCAATTAATTCT+CGG + chr2_1:58263987-58264006 Msa0214090:intron 30.0%
GGCTATTATAGTTCATTTGA+TGG - chr2_1:58264152-58264171 None:intergenic 30.0%
TAATGAGTAGATATAAGGCT+AGG - chr2_1:58264173-58264192 None:intergenic 30.0%
TGTATTACAATCAGAGACAA+TGG + chr2_1:58264282-58264301 Msa0214090:intron 30.0%
!! TTTCCCATTTAACTTGTTGT+TGG + chr2_1:58264387-58264406 Msa0214090:intron 30.0%
!! TTCCCATTTAACTTGTTGTT+GGG + chr2_1:58264388-58264407 Msa0214090:intron 30.0%
!! GAGAAGTATTTGAATTTGTC+TGG + chr2_1:58264640-58264659 Msa0214090:intron 30.0%
GAACCTACATACTAATAAGT+TGG + chr2_1:58264722-58264741 Msa0214090:intron 30.0%
CATCCAACTTATTAGTATGT+AGG - chr2_1:58264728-58264747 None:intergenic 30.0%
! CATACTAATAAGTTGGATGA+AGG + chr2_1:58264729-58264748 Msa0214090:intron 30.0%
!! TTACTAGTAGAAGTACTCAT+TGG - chr2_1:58265150-58265169 None:intergenic 30.0%
GGACAATATAGTTATGTTGA+TGG + chr2_1:58265231-58265250 Msa0214090:CDS 30.0%
AGAGAAATATTCTATGTAGG+TGG + chr2_1:58265355-58265374 Msa0214090:three_prime_UTR 30.0%
ACTTTATTGTTGTGTCTCTT+TGG + chr2_1:58265465-58265484 Msa0214090:three_prime_UTR 30.0%
ATTGTATATGTCAAACACTG+AGG - chr2_1:58265515-58265534 None:intergenic 30.0%
TAAACAAAACACACCACAAC+AGG - chr2_1:58262744-58262763 None:intergenic 35.0%
!!! TGGTGTGTTTTGTTTATTGG+TGG + chr2_1:58262748-58262767 Msa0214090:five_prime_UTR 35.0%
!! AGCTGTTCTGCGTTTTAATA+TGG + chr2_1:58262776-58262795 Msa0214090:five_prime_UTR 35.0%
!! GCTGTTCTGCGTTTTAATAT+GGG + chr2_1:58262777-58262796 Msa0214090:five_prime_UTR 35.0%
!! CTGCGTTTTAATATGGGTAT+TGG + chr2_1:58262783-58262802 Msa0214090:intron 35.0%
!! TTTGGTGCATAACTGTGTTT+TGG + chr2_1:58262807-58262826 Msa0214090:intron 35.0%
!! AGCTGCTAGATTTATTTTCG+TGG + chr2_1:58262847-58262866 Msa0214090:intron 35.0%
!! GCTAGATTTATTTTCGTGGA+TGG + chr2_1:58262851-58262870 Msa0214090:intron 35.0%
! TGTCTTTGTAGAAGCTTGTT+TGG + chr2_1:58262877-58262896 Msa0214090:intron 35.0%
GCTTTGTATTTCTTCTGTTG+AGG + chr2_1:58262938-58262957 Msa0214090:intron 35.0%
TTCCTATTGAATTTGGCACT+TGG + chr2_1:58263054-58263073 Msa0214090:intron 35.0%
TTCCAAGTGCCAAATTCAAT+AGG - chr2_1:58263059-58263078 None:intergenic 35.0%
GAATCAGCCAAAAGGAAAAT+GGG - chr2_1:58263144-58263163 None:intergenic 35.0%
AGAATCAGCCAAAAGGAAAA+TGG - chr2_1:58263145-58263164 None:intergenic 35.0%
AGAAATAACGGTAACTCTTG+TGG + chr2_1:58263631-58263650 Msa0214090:intron 35.0%
AGTGAGAAGATTGAAACTAC+TGG + chr2_1:58263675-58263694 Msa0214090:intron 35.0%
TGAGATGAGAGTAGATTTCA+AGG + chr2_1:58263716-58263735 Msa0214090:intron 35.0%
! GTGCTTTAATCTCTTTGAGA+AGG + chr2_1:58263749-58263768 Msa0214090:intron 35.0%
TAGTGTTAAATCAGTGGTCA+TGG - chr2_1:58263891-58263910 None:intergenic 35.0%
GCTCCTATACTCATAAGTTA+AGG - chr2_1:58263960-58263979 None:intergenic 35.0%
TATGAGTATAGGAGCTAGTT+TGG + chr2_1:58263965-58263984 Msa0214090:intron 35.0%
AACAGTGTTGTGATTGACAA+TGG + chr2_1:58264338-58264357 Msa0214090:intron 35.0%
GACCCAACAACAAGTTAAAT+GGG - chr2_1:58264393-58264412 None:intergenic 35.0%
TGACCCAACAACAAGTTAAA+TGG - chr2_1:58264394-58264413 None:intergenic 35.0%
!! CTGGTTCTAGATTTCAAGAA+TGG + chr2_1:58264659-58264678 Msa0214090:intron 35.0%
TGTTGTGATATCAGCATTGT+TGG + chr2_1:58264837-58264856 Msa0214090:intron 35.0%
AACTCCAATAGTGCTAAAAG+TGG - chr2_1:58264953-58264972 None:intergenic 35.0%
!! TTTTTCTCTGGGAGTGTATA+TGG + chr2_1:58265060-58265079 Msa0214090:intron 35.0%
!! AGAAGTACGAGTTCTGAAAA+AGG + chr2_1:58265109-58265128 Msa0214090:five_prime_UTR 35.0%
!! CGAGTTCTGAAAAAGGAAAT+TGG + chr2_1:58265116-58265135 Msa0214090:five_prime_UTR 35.0%
!! GTTCTGAAAAAGGAAATTGG+AGG + chr2_1:58265119-58265138 Msa0214090:five_prime_UTR 35.0%
TATTGTTGTGTCTCTTTGGA+AGG + chr2_1:58265469-58265488 Msa0214090:three_prime_UTR 35.0%
! ATTGTTGTGTCTCTTTGGAA+GGG + chr2_1:58265470-58265489 Msa0214090:three_prime_UTR 35.0%
CTATCCTTTCTAACCATGAG+TGG + chr2_1:58262560-58262579 Msa0214090:five_prime_UTR 40.0%
! TAGGACTTTGTTTCCTTTGG+TGG + chr2_1:58262713-58262732 Msa0214090:five_prime_UTR 40.0%
! TTGTAGAAGCTTGTTTGGCT+TGG + chr2_1:58262882-58262901 Msa0214090:intron 40.0%
!! AAGCTTGTTTGGCTTGGTTT+GGG + chr2_1:58262888-58262907 Msa0214090:intron 40.0%
ATCTCTGTACTTCTTGTGCA+GGG + chr2_1:58262962-58262981 Msa0214090:intron 40.0%
AGAGTGGTGAAGTTAGAGTA+AGG - chr2_1:58263495-58263514 None:intergenic 40.0%
AAATGTCCTGATTGGTAGAG+TGG - chr2_1:58263511-58263530 None:intergenic 40.0%
GCAACAACAAATGTCCTGAT+TGG - chr2_1:58263519-58263538 None:intergenic 40.0%
AGTGAGTACTCTTCAACCAA+AGG + chr2_1:58263542-58263561 Msa0214090:five_prime_UTR 40.0%
TGGACAATGGTTCATACCTT+TGG - chr2_1:58263561-58263580 None:intergenic 40.0%
TGGCTATGACTATTGGACAA+TGG - chr2_1:58263574-58263593 None:intergenic 40.0%
CAATGCATGGCTATGACTAT+TGG - chr2_1:58263581-58263600 None:intergenic 40.0%
! GAAACTACTGGCTGATTCAA+TGG + chr2_1:58263687-58263706 Msa0214090:intron 40.0%
CATTTCAGTCTAGCCATTTC+TGG + chr2_1:58263913-58263932 Msa0214090:intron 40.0%
! TTGACAATGGCTAGTCAACA+AGG + chr2_1:58264351-58264370 Msa0214090:intron 40.0%
ACAAGCATGTGATTGATGTG+TGG + chr2_1:58264462-58264481 Msa0214090:intron 40.0%
TGTGATTGATGTGTGGTTCT+CGG + chr2_1:58264469-58264488 Msa0214090:intron 40.0%
! ATAAGTTGGATGAAGGCCTA+TGG + chr2_1:58264736-58264755 Msa0214090:intron 40.0%
!! GCTTCCACTTTTAGCACTAT+TGG + chr2_1:58264946-58264965 Msa0214090:intron 40.0%
TGAAAAAGGAAATTGGAGGC+TGG + chr2_1:58265123-58265142 Msa0214090:five_prime_UTR 40.0%
ATCTCCACAGTTGCATAGTA+TGG + chr2_1:58265207-58265226 Msa0214090:CDS 40.0%
!! AGTTATGTTGATGGTTCAGC+AGG + chr2_1:58265240-58265259 Msa0214090:CDS 40.0%
ATATTCTATGTAGGTGGCCT+AGG + chr2_1:58265361-58265380 Msa0214090:three_prime_UTR 40.0%
GATGCCACTCATGGTTAGAA+AGG - chr2_1:58262567-58262586 None:intergenic 45.0%
CAAAAACGAGATGCCACTCA+TGG - chr2_1:58262576-58262595 None:intergenic 45.0%
! CATGAGTGGCATCTCGTTTT+TGG + chr2_1:58262574-58262593 Msa0214090:five_prime_UTR 45.0%
ACAACAGGTACATCCACCAA+AGG - chr2_1:58262729-58262748 None:intergenic 45.0%
! TTTGGTGGATGTACCTGTTG+TGG + chr2_1:58262728-58262747 Msa0214090:five_prime_UTR 45.0%
TTAAAACGCAGAACAGCTGC+AGG - chr2_1:58262774-58262793 None:intergenic 45.0%
!! GAAGCTTGTTTGGCTTGGTT+TGG + chr2_1:58262887-58262906 Msa0214090:intron 45.0%
CATCTCTGTACTTCTTGTGC+AGG + chr2_1:58262961-58262980 Msa0214090:intron 45.0%
ACTTCACCACTCTACCAATC+AGG + chr2_1:58263502-58263521 Msa0214090:five_prime_UTR 45.0%
TCCACAGTTGCATAGTATGG+AGG + chr2_1:58265210-58265229 Msa0214090:CDS 45.0%
TCCTCCATACTATGCAACTG+TGG - chr2_1:58265214-58265233 None:intergenic 45.0%
AGGTCCTCAATCAAGATCCT+AGG - chr2_1:58265381-58265400 None:intergenic 45.0%
AAACGGGCACCAAACAAAGG+CGG - chr2_1:58262918-58262937 None:intergenic 50.0%
AGCAAACGGGCACCAAACAA+AGG - chr2_1:58262921-58262940 None:intergenic 50.0%
TAGCTGGTAAATGGGGGAGT+TGG + chr2_1:58263810-58263829 Msa0214090:intron 50.0%
!! AGTCTAGCCATTTCTGGCCA+AGG + chr2_1:58263919-58263938 Msa0214090:intron 50.0%
!! GTCTAGCCATTTCTGGCCAA+GGG + chr2_1:58263920-58263939 Msa0214090:intron 50.0%
TGATGTGTGGTTCTCGGTGA+AGG + chr2_1:58264475-58264494 Msa0214090:intron 50.0%
TGGTTCTCGGTGAAGGAAGA+TGG + chr2_1:58264482-58264501 Msa0214090:intron 50.0%
GGTGAAGGAAGATGGAGTGT+TGG + chr2_1:58264490-58264509 Msa0214090:intron 50.0%
GTGGCCTAGGATCTTGATTG+AGG + chr2_1:58265374-58265393 Msa0214090:three_prime_UTR 50.0%
! TGTGTCTCTTTGGAAGGGAG+TGG + chr2_1:58265475-58265494 Msa0214090:three_prime_UTR 50.0%
TTGGGTGTGCCGCCTTTGTT+TGG + chr2_1:58262906-58262925 Msa0214090:intron 55.0%
GCATGTGGGTCTACACACAC+TGG + chr2_1:58263360-58263379 Msa0214090:intron 55.0%
TGTGCGCCCTTGGCCAGAAA+TGG - chr2_1:58263929-58263948 None:intergenic 60.0%
!! GGGAAAGTGTTGTGCGCCCT+TGG - chr2_1:58263939-58263958 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 58262467 58265871 58262467 ID=Msa0214090;Name=Msa0214090
chr2_1 mRNA 58262467 58265871 58262467 ID=Msa0214090-mRNA-1;Parent=Msa0214090;Name=Msa0214090-mRNA-1;_AED=0.27;_eAED=0.27;_QI=539|0.66|1|1|0|0.25|4|569|34
chr2_1 exon 58262467 58262798 58262467 ID=Msa0214090-mRNA-1:exon:16037;Parent=Msa0214090-mRNA-1
chr2_1 exon 58263461 58263563 58263461 ID=Msa0214090-mRNA-1:exon:16038;Parent=Msa0214090-mRNA-1
chr2_1 exon 58265074 58265782 58265074 ID=Msa0214090-mRNA-1:exon:16039;Parent=Msa0214090-mRNA-1
chr2_1 exon 58265803 58265871 58265803 ID=Msa0214090-mRNA-1:exon:16040;Parent=Msa0214090-mRNA-1
chr2_1 five_prime_UTR 58262467 58262798 58262467 ID=Msa0214090-mRNA-1:five_prime_utr;Parent=Msa0214090-mRNA-1
chr2_1 five_prime_UTR 58263461 58263563 58263461 ID=Msa0214090-mRNA-1:five_prime_utr;Parent=Msa0214090-mRNA-1
chr2_1 five_prime_UTR 58265074 58265177 58265074 ID=Msa0214090-mRNA-1:five_prime_utr;Parent=Msa0214090-mRNA-1
chr2_1 CDS 58265178 58265282 58265178 ID=Msa0214090-mRNA-1:cds;Parent=Msa0214090-mRNA-1
chr2_1 three_prime_UTR 58265283 58265782 58265283 ID=Msa0214090-mRNA-1:three_prime_utr;Parent=Msa0214090-mRNA-1
chr2_1 three_prime_UTR 58265803 58265871 58265803 ID=Msa0214090-mRNA-1:three_prime_utr;Parent=Msa0214090-mRNA-1
Gene Sequence

>Msa0214090

ATGGTTTTATTGGTAGTTATAAGGCTCATATCTCCACAGTTGCATAGTATGGAGGACAATATAGTTATGTTGATGGTTCAGCAGGTATGTTATAATATTTTGTAG
Protein sequence

>Msa0214090

MVLLVVIRLISPQLHSMEDNIVMLMVQQVCYNIL*