AlfalfaGEDB | Msa0224120

M. sativa cultivar zhongmu-4 / Msa0224120

KEGG: K02218
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224120	XP_003597565.1	99.789	475	1	0	1	475	1	475	0.0	979

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224120	sp\|Q9CAI5\|CKL2_ARATH	78.038	469	98	4	1	468	1	465	0.0	738

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224120	G7IS36	99.789	475	1	0	1	475	1	475	0.0	979

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0224120	Msa0265260	0.974181	1.047293e-137	-8.615850e-47
Msa0224120	Msa1089200	0.955108	6.473554e-113	-8.615850e-47
Msa0224120	Msa1364090	0.968610	6.374658e-129	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224120	MtrunA17_Chr2g0329981	99.789	475	1	0	1	475	1	475	0.0	979
Msa0224120	MtrunA17_Chr8g0342821	83.229	477	75	5	1	475	1	474	0.0	808
Msa0224120	MtrunA17_Chr4g0071031	75.681	477	91	5	1	475	1	454	0.0	706
Msa0224120	MtrunA17_Chr2g0299661	69.787	470	138	3	1	470	1	466	0.0	677
Msa0224120	MtrunA17_Chr1g0183631	60.842	475	156	9	1	468	1	452	0.0	554
Msa0224120	MtrunA17_Chr7g0261501	73.639	349	90	1	1	349	1	347	0.0	550
Msa0224120	MtrunA17_Chr1g0166821	74.432	352	80	3	1	349	2	346	0.0	547
Msa0224120	MtrunA17_Chr1g0187571	69.474	380	109	3	1	380	2	374	0.0	536
Msa0224120	MtrunA17_Chr6g0486611	63.007	419	139	7	1	411	1	411	0.0	516
Msa0224120	MtrunA17_Chr8g0390211	63.706	394	132	5	1	392	1	385	9.63e-180	509

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0224120	AT1G72710.1	78.038	469	98	4	1	468	1	465	738
Msa0224120	AT4G26100.1	71.550	471	109	5	1	471	1	446	677
Msa0224120	AT5G57015.1	69.538	476	102	6	1	475	1	434	664
Msa0224120	AT2G19470.1	82.569	327	57	0	1	327	1	327	590
Msa0224120	AT3G23340.1	73.371	353	83	3	1	349	1	346	545
Msa0224120	AT5G44100.1	81.818	297	54	0	1	297	1	297	541
Msa0224120	AT5G44100.2	81.818	297	54	0	1	297	1	297	541
Msa0224120	AT1G03930.1	81.481	297	55	0	1	297	1	297	541
Msa0224120	AT4G14340.1	65.625	416	120	7	1	411	7	404	539
Msa0224120	AT4G28540.1	60.131	459	164	6	1	449	5	454	530

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Find 150 sgRNAs with CRISPR-Local

Find 418 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGTAGCGGATCCTT+CGG	0.514691	2_1:-73881844	Msa0224120:CDS
AAACCAGACAACTTCCTCAT+GGG	0.585296	2_1:-73880434	Msa0224120:CDS
AAACCCTAGAAATTGGGAAT+TGG	0.406920	2_1:+73881928	None:intergenic
AAATATTCAGACAAATGAAG+AGG	0.611556	2_1:-73881704	Msa0224120:CDS
AAATGGATCTAAAGAAGTTA+TGG	0.456366	2_1:-73877374	Msa0224120:intron
AAATTCACCTATCACATCAT+CGG	0.605560	2_1:-73876617	Msa0224120:CDS
AACAACTGCATCACGACTGT+TGG	0.471395	2_1:+73876720	None:intergenic
AACCCTCTCTTCCTCTTGCC+AGG	0.341730	2_1:-73876684	Msa0224120:CDS
AAGATGGTAGACATGCTGGT+TGG	0.660444	2_1:-73877489	Msa0224120:CDS
AAGGAGTTGGTGAAGAGAAA+GGG	0.465579	2_1:+73881964	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAGAGTTTCTCTCAC+TGG	+	chr2_1:73876285-73876304	None:intergenic	30.0%
AATTGGAGATTGGAAATTGA+AGG	+	chr2_1:73876390-73876409	None:intergenic	30.0%
CCTAATAAAACCCTAGAAAT+TGG	+	chr2_1:73876414-73876433	None:intergenic	30.0%
AACAAAACTTGCATGTTTGT+TGG	-	chr2_1:73876728-73876747	Msa0224120:CDS	30.0%
ACATTCATGTAAAAGTGAGT+TGG	-	chr2_1:73876872-73876891	Msa0224120:intron	30.0%
GCTTCAAGTAGAATCAATTA+TGG	-	chr2_1:73876948-73876967	Msa0224120:intron	30.0%
TCAAGTAGAATCAATTATGG+AGG	-	chr2_1:73876951-73876970	Msa0224120:intron	30.0%
AAGCCAAAATCAATTCTAGA+GGG	+	chr2_1:73877027-73877046	None:intergenic	30.0%
AGAGGAATTGATGTATCTAA+AGG	-	chr2_1:73877181-73877200	Msa0224120:intron	30.0%
CGACAAATTAAATTAGACAC+AGG	+	chr2_1:73877239-73877258	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	73876086	73882268	73876086	ID=Msa0224120;Name=Msa0224120
chr2_1	mRNA	73876086	73882268	73876086	ID=Msa0224120-mRNA-1;Parent=Msa0224120;Name=Msa0224120-mRNA-1;_AED=0.15;_eAED=0.15;_QI=363\|1\|1\|1\|1\|1\|14\|418\|475
chr2_1	exon	73876086	73876800	73876086	ID=Msa0224120-mRNA-1:exon:21771;Parent=Msa0224120-mRNA-1
chr2_1	exon	73877375	73877517	73877375	ID=Msa0224120-mRNA-1:exon:21770;Parent=Msa0224120-mRNA-1
chr2_1	exon	73877752	73877815	73877752	ID=Msa0224120-mRNA-1:exon:21769;Parent=Msa0224120-mRNA-1
chr2_1	exon	73877897	73877979	73877897	ID=Msa0224120-mRNA-1:exon:21768;Parent=Msa0224120-mRNA-1
chr2_1	exon	73878108	73878234	73878108	ID=Msa0224120-mRNA-1:exon:21767;Parent=Msa0224120-mRNA-1
chr2_1	exon	73878323	73878407	73878323	ID=Msa0224120-mRNA-1:exon:21766;Parent=Msa0224120-mRNA-1
chr2_1	exon	73878492	73878555	73878492	ID=Msa0224120-mRNA-1:exon:21765;Parent=Msa0224120-mRNA-1
chr2_1	exon	73878651	73878712	73878651	ID=Msa0224120-mRNA-1:exon:21764;Parent=Msa0224120-mRNA-1
chr2_1	exon	73880244	73880314	73880244	ID=Msa0224120-mRNA-1:exon:21763;Parent=Msa0224120-mRNA-1
chr2_1	exon	73880412	73880507	73880412	ID=Msa0224120-mRNA-1:exon:21762;Parent=Msa0224120-mRNA-1
chr2_1	exon	73880592	73880740	73880592	ID=Msa0224120-mRNA-1:exon:21761;Parent=Msa0224120-mRNA-1
chr2_1	exon	73880850	73880919	73880850	ID=Msa0224120-mRNA-1:exon:21760;Parent=Msa0224120-mRNA-1
chr2_1	exon	73881690	73881730	73881690	ID=Msa0224120-mRNA-1:exon:21759;Parent=Msa0224120-mRNA-1
chr2_1	exon	73881830	73882268	73881830	ID=Msa0224120-mRNA-1:exon:21758;Parent=Msa0224120-mRNA-1
chr2_1	five_prime_UTR	73881906	73882268	73881906	ID=Msa0224120-mRNA-1:five_prime_utr;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73881830	73881905	73881830	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73881690	73881730	73881690	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73880850	73880919	73880850	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73880592	73880740	73880592	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73880412	73880507	73880412	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73880244	73880314	73880244	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73878651	73878712	73878651	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73878492	73878555	73878492	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73878323	73878407	73878323	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73878108	73878234	73878108	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73877897	73877979	73877897	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73877752	73877815	73877752	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73877375	73877517	73877375	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	CDS	73876504	73876800	73876504	ID=Msa0224120-mRNA-1:cds;Parent=Msa0224120-mRNA-1
chr2_1	three_prime_UTR	73876086	73876503	73876086	ID=Msa0224120-mRNA-1:three_prime_utr;Parent=Msa0224120-mRNA-1

Gene Sequence

>Msa0224120

ATGGAACCTCGTATTGGTAACAAGTTCCGTCTTGGTCGCAAAATTGGTAGCGGATCCTTCGGCGAGATCTATCTCGGTACAAATATTCAGACAAATGAAGAGGTTGCAATTAAGCTCGAAAATATCAAAACCAAGCATCCTCAGTTGTTGTACGAATCAAAGTTGTATAAAGTACTTCAAGGCGGAACTGGTATCCCGAATGTGAGATGGTTTGGCGTAGAAGGAGAGTACAATGTTCTTGTAATGGATTTACTGGGACCAAGTCTCGAGGATTTATTCAGTTTTTGTAGTCGGAAATTGTCCCTTAAAACTGTACTCATGCTGGCTGATCAGATGATAAATCGAGTTGAATATATTCATTCCAAATCATTTTTACATAGGGACATCAAACCAGACAACTTCCTCATGGGTCTGGGGAGGCGAGCTAATCAAGTGTATGCCATTGACTTCGGTCTTGCTAAAAAATACAGGGACACCTCTACCCATCAGCATATACCTTACAGAGAGAATAAGAACTTGACAGGAACTGCTAGATATGCTAGTATGAATACACATCTTGGAATTGAGCAAAGCCGTAGGGACGACTTAGAATCACTTGGATATGTTCTTATGTACTTTTTGAGAGGAAGCCTTCCATGGCAGGGATTAAAAGCAGGTACTAAAAAGCAGAAGTACGAGAAAATCAGTGAGAAGAAAGTTTCTACTTCTATTGAGTCCTTGTGTCGTGGCTACCCCTCAGAATTCGCTTCGTACTTCCATTACTGTCGGTCATTGCGGTTTGATGATAAACCAGATTATGCATATCTGAAAAGGCTGTTTCGCGACCTTTTCATTCGTGAAGGATTCCAGTTTGATTATGTCTTCGATTGGACCATTTTGAAGTATCAGCAATCTCAAATTGCCACTCCTTCTGCACGTGCCATTGGTTCTGGTGCTGGGCCAAGTTCTGGCTTACCACCAGCTGTTGTGAGTGCTGAGAGACCATCAGGTGGGGAAGATGGTAGACATGCTGGTTGGTCTTCATCAGATCCTGCTCGTAGAAGAAACTCTGGACCTATTGTAAATGATGGAAACTTGTCTAGACAAAAAGCACCGGTACCAAGTGACTCAAATGGATCTAAAGAAGTTATGTTGTCTAGTTCTAATTTCTTCCGGCCTAGTGGATCTACAAGACGAGTAGGTGCTGTTTCCAACAGTCGTGATGCAGTTGTTAGCAGTGAAATTGAACCCTCTCTTCCTCTTGCCAGGGATGGTAGTCCTGGAGCACTCCTTAAAACCTCTGGTGCTCAAAGAAATTCACCTATCACATCATCGGAGCACAAAGCAACAACATCTTCTGGCAGAAACACATCAAACATGAAGAATTTTGAGTCAACTATCCGAGGTATTGAGAGCCTGAATTTCAATGACGAGAAGGTACAGTATTAG

Protein sequence

>Msa0224120

MEPRIGNKFRLGRKIGSGSFGEIYLGTNIQTNEEVAIKLENIKTKHPQLLYESKLYKVLQGGTGIPNVRWFGVEGEYNVLVMDLLGPSLEDLFSFCSRKLSLKTVLMLADQMINRVEYIHSKSFLHRDIKPDNFLMGLGRRANQVYAIDFGLAKKYRDTSTHQHIPYRENKNLTGTARYASMNTHLGIEQSRRDDLESLGYVLMYFLRGSLPWQGLKAGTKKQKYEKISEKKVSTSIESLCRGYPSEFASYFHYCRSLRFDDKPDYAYLKRLFRDLFIREGFQFDYVFDWTILKYQQSQIATPSARAIGSGAGPSSGLPPAVVSAERPSGGEDGRHAGWSSSDPARRRNSGPIVNDGNLSRQKAPVPSDSNGSKEVMLSSSNFFRPSGSTRRVGAVSNSRDAVVSSEIEPSLPLARDGSPGALLKTSGAQRNSPITSSEHKATTSSGRNTSNMKNFESTIRGIESLNFNDEKVQY*