AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0227180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0227180 MtrunA17_Chr3g0125261 98.502 267 4 0 1 267 1 267 0.0 547
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0227180 AT1G77290.3 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa0227180 AT1G77290.1 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa0227180 AT1G77290.2 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa0227180 AT4G02520.1 24.280 243 143 4 1 237 4 211 7.43e-14 69.3

Find 98 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGTCAAATTTCTGTTAT+TGG 0.115960 2_1:+77871117 Msa0227180:five_prime_UTR
CATTGCTGAGTATTGGATTT+TGG 0.152263 2_1:+77872599 Msa0227180:CDS
TCCTGTAACTGGCAAGAATT+TGG 0.171996 2_1:+77871641 Msa0227180:CDS
TTTAGCTCGTTTAAAACTTT+TGG 0.180573 2_1:+77872542 Msa0227180:CDS
ACTGAGTGAAACACCTTATT+TGG 0.190936 2_1:+77872476 Msa0227180:CDS
TGATATCACTTCTTACTTAT+AGG 0.210386 2_1:+77871227 Msa0227180:five_prime_UTR
CTCGGTGTTTCTCTGGTATA+TGG 0.211375 2_1:-77872269 None:intergenic
AAAATTGATATATTAATAAA+TGG 0.234552 2_1:-77871198 None:intergenic
TCCAAATTCTTGCCAGTTAC+AGG 0.236554 2_1:-77871642 None:intergenic
AACTCTTCGCCTGCCAAATA+AGG 0.264517 2_1:-77872489 None:intergenic
TTACTTATTCAACCATTTCT+TGG 0.265920 2_1:-77872770 None:intergenic
TACTTTAACAAACCCTTTAT+CGG 0.276702 2_1:+77870816 Msa0227180:five_prime_UTR
AGGCTTCTCTCAACTTTCTA+TGG 0.276841 2_1:-77872365 None:intergenic
TAATCAAAGATGTTGAATAT+TGG 0.278227 2_1:+77870940 Msa0227180:five_prime_UTR
TGGTATGCACCTGCTAATTC+AGG 0.285480 2_1:-77872345 None:intergenic
ATATCATCATCCGTTTGATT+TGG 0.293342 2_1:+77871554 Msa0227180:CDS
TATAGGCTTCTGTCACGTTT+TGG 0.294407 2_1:+77871244 Msa0227180:five_prime_UTR
CGGATTCATCTGAAAGAATA+AGG 0.297726 2_1:-77871667 None:intergenic
ACTTCAGTACTCTTTATATT+AGG 0.305891 2_1:+77871011 Msa0227180:five_prime_UTR
CCCTGAACTGTTTCAGTACT+TGG 0.317517 2_1:-77873014 None:intergenic
TACTTGGTCCAATGAAATTC+TGG 0.327240 2_1:-77872998 None:intergenic
TTTACGTTTCCAAGTTCTTA+AGG 0.342669 2_1:+77872292 Msa0227180:CDS
CCAATGCAAGTCTCACCTTC+TGG 0.346297 2_1:-77871581 None:intergenic
TGTGTATTATTGATTGATAT+TGG 0.347592 2_1:+77873117 Msa0227180:three_prime_UTR
AAATGATGTGAGAGCCGTTT+TGG 0.353112 2_1:-77871710 None:intergenic
AGACTTGCATTGGAAGAAAA+AGG 0.354375 2_1:+77871591 Msa0227180:CDS
GTTATGTGTGACACTAGTTT+TGG 0.361960 2_1:+77873170 Msa0227180:three_prime_UTR
TGGAACAATGCCTTCTTGTA+AGG 0.374184 2_1:+77873137 Msa0227180:three_prime_UTR
TTCCATACCAATCCTGTAAC+TGG 0.385351 2_1:+77871630 Msa0227180:CDS
GAGAAGGAGCGAGGAGCATT+TGG 0.386161 2_1:+77872425 Msa0227180:CDS
GTTTGTCTCCAGAATTTCAT+TGG 0.390685 2_1:+77872990 Msa0227180:three_prime_UTR
CACCACACGCCTTAAGAACT+TGG 0.391896 2_1:-77872301 None:intergenic
TTTATTTCTCCCTTACTGCT+TGG 0.399542 2_1:+77872890 Msa0227180:three_prime_UTR
CGAGACAATGTAAGAAAGAT+TGG 0.402140 2_1:-77871284 None:intergenic
ACCAAGTACTGAAACAGTTC+AGG 0.403387 2_1:+77873013 Msa0227180:three_prime_UTR
CTCTCATGGTTGCCAAGAAA+TGG 0.406293 2_1:+77872758 Msa0227180:three_prime_UTR
ATCCAATACTCAGCAATGTT+TGG 0.413409 2_1:-77872594 None:intergenic
ACGTAAACTCGGTGTTTCTC+TGG 0.433917 2_1:-77872276 None:intergenic
ATATTTGATATTTCAATATG+TGG 0.434883 2_1:+77871158 Msa0227180:five_prime_UTR
GGCCAAACATTGCTGAGTAT+TGG 0.435036 2_1:+77872592 Msa0227180:CDS
CCACCTAGGAAGTTGCTGAT+AGG 0.448934 2_1:-77871513 None:intergenic
AGAATTGCTGTGGTCTCCGC+AGG 0.450946 2_1:+77872159 Msa0227180:CDS
CTGAGGATATCAGTAGCAAT+AGG 0.470164 2_1:+77872184 Msa0227180:CDS
TTCACATTATTCTGTCGTAA+AGG 0.472557 2_1:-77871327 None:intergenic
ATTCTTGCCAGTTACAGGAT+TGG 0.485946 2_1:-77871637 None:intergenic
ACTAACATCACCAAGCAGTA+AGG 0.489289 2_1:-77872900 None:intergenic
GTTGTTGTTTATTGACAACT+TGG 0.490653 2_1:+77871402 Msa0227180:five_prime_UTR
TTCTGGCTGTCCAAATCAAA+CGG 0.493106 2_1:-77871564 None:intergenic
TTTGCTTAAGCGGTACTAGT+AGG 0.493567 2_1:+77872725 Msa0227180:exon
ACAAAACATTGTTCAAAACT+TGG 0.494072 2_1:+77872682 Msa0227180:CDS
TGCCAGTTACAGGATTGGTA+TGG 0.497143 2_1:-77871632 None:intergenic
AGCACTAAAATGAAATGAAA+GGG 0.508133 2_1:-77871071 None:intergenic
GCAATCACCTTTCTATAACT+AGG 0.510277 2_1:-77872633 None:intergenic
ATATTAGAACTTTGCTTAAG+CGG 0.513812 2_1:+77872715 Msa0227180:CDS
GGTTAGATTGCTTGATGAAG+CGG 0.515391 2_1:+77872446 Msa0227180:CDS
TAGCACTAAAATGAAATGAA+AGG 0.516270 2_1:-77871072 None:intergenic
ATACCTATCAGCAACTTCCT+AGG 0.523035 2_1:+77871510 Msa0227180:five_prime_UTR
GGTATGCACCTGCTAATTCA+GGG 0.526259 2_1:-77872344 None:intergenic
TCGGTGTTTCTCTGGTATAT+GGG 0.528301 2_1:-77872268 None:intergenic
ATGAGAAGGGAGGAAAATCA+AGG 0.529179 2_1:+77872953 Msa0227180:three_prime_UTR
GGATGAAGAGGATACAAGAG+TGG 0.530840 2_1:+77872223 Msa0227180:CDS
AGTGAAACACCTTATTTGGC+AGG 0.533423 2_1:+77872480 Msa0227180:CDS
TGTGGTGATCGCTCGTATGT+CGG 0.536691 2_1:+77872317 Msa0227180:CDS
ACAATGTAAGAAAGATTGGC+AGG 0.541598 2_1:-77871280 None:intergenic
TCGGAGTCCCCTGAATTAGC+AGG 0.546117 2_1:+77872336 Msa0227180:CDS
TGTGGTCTCCGCAGGGTCTG+AGG 0.546519 2_1:+77872167 Msa0227180:CDS
CCAGAAGGTGAGACTTGCAT+TGG 0.548807 2_1:+77871581 Msa0227180:CDS
GCAGAGGCCTAGTTATAGAA+AGG 0.552401 2_1:+77872626 Msa0227180:CDS
ATAGGAGTGAAGTGATTGAA+TGG 0.553982 2_1:+77872202 Msa0227180:CDS
AATCAAGGTATGCATGTAAG+TGG 0.555188 2_1:+77872968 Msa0227180:three_prime_UTR
TTCCAAGTTCTTAAGGCGTG+TGG 0.557777 2_1:+77872299 Msa0227180:CDS
GTTTCTCTGGTATATGGGAG+AGG 0.560412 2_1:-77872263 None:intergenic
CACTTATCCTAGAATGAGAA+GGG 0.565199 2_1:+77872940 Msa0227180:three_prime_UTR
CTAACATCACCAAGCAGTAA+GGG 0.567559 2_1:-77872899 None:intergenic
CATTGTCAAAAGAAAAGAAG+TGG 0.576035 2_1:+77873051 Msa0227180:three_prime_UTR
GGGTGAAATAAAGTAGCCAT+TGG 0.581067 2_1:-77873242 None:intergenic
AGAATGAGTACATAACTATG+AGG 0.584947 2_1:+77872571 Msa0227180:CDS
CGCTCCTTCTCAAAACATGA+GGG 0.586987 2_1:-77872413 None:intergenic
CCAAGTACTGAAACAGTTCA+GGG 0.589412 2_1:+77873014 Msa0227180:three_prime_UTR
GCACTTATCCTAGAATGAGA+AGG 0.590458 2_1:+77872939 Msa0227180:three_prime_UTR
TCGCTCCTTCTCAAAACATG+AGG 0.593199 2_1:-77872414 None:intergenic
AAGCAGCAATCCTTACAAGA+AGG 0.594753 2_1:-77873147 None:intergenic
GGCAGGCGAAGAGTTTAGCA+TGG 0.595558 2_1:+77872497 Msa0227180:CDS
GTTTGATTTGGACAGCCAGA+AGG 0.595603 2_1:+77871566 Msa0227180:CDS
TAGGTACATATATACTCTCA+TGG 0.604593 2_1:+77872744 Msa0227180:three_prime_UTR
AAAACAGGAAGGCTTCCACT+CGG 0.612067 2_1:-77871687 None:intergenic
TTTCTCTGGTATATGGGAGA+GGG 0.623180 2_1:-77872262 None:intergenic
GATGAAGAGGATACAAGAGT+GGG 0.626925 2_1:+77872224 Msa0227180:CDS
AGAACTTGGAAACGTAAACT+CGG 0.634065 2_1:-77872287 None:intergenic
GTACATAGAAAGAATTGCTG+TGG 0.635061 2_1:+77872149 Msa0227180:CDS
TAGATCAAGAACGTAACCAA+TGG 0.635563 2_1:+77873226 Msa0227180:three_prime_UTR
AAGTGATTGAATGGATGAAG+AGG 0.651845 2_1:+77872211 Msa0227180:CDS
TTCTTTCAGATGAATCCGAG+TGG 0.664252 2_1:+77871672 Msa0227180:CDS
GAATTGCTGTGGTCTCCGCA+GGG 0.673553 2_1:+77872160 Msa0227180:CDS
GTATGCACCTGCTAATTCAG+GGG 0.678849 2_1:-77872343 None:intergenic
TTATCCTAGAATGAGAAGGG+AGG 0.687206 2_1:+77872943 Msa0227180:three_prime_UTR
CCTATCAGCAACTTCCTAGG+TGG 0.692964 2_1:+77871513 Msa0227180:five_prime_UTR
ACTGATATCCTCAGACCCTG+CGG 0.699635 2_1:-77872175 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGATATTTCAATATG+TGG + chr2_1:77871158-77871177 Msa0227180:five_prime_UTR 15.0%
!! TTTATCTTTAAACTACTATA+TGG - chr2_1:77871869-77871888 None:intergenic 15.0%
!! TCAACAAAAATAAAAAGATA+GGG - chr2_1:77872851-77872870 None:intergenic 15.0%
!! TTCAACAAAAATAAAAAGAT+AGG - chr2_1:77872852-77872871 None:intergenic 15.0%
!!! TTTATTTTTGTTGAAATAGA+AGG + chr2_1:77872857-77872876 Msa0227180:three_prime_UTR 15.0%
!!! TTATTTTTGTTGAAATAGAA+GGG + chr2_1:77872858-77872877 Msa0227180:three_prime_UTR 15.0%
!! AAAAATCAATAAATTCAGTG+AGG - chr2_1:77870774-77870793 None:intergenic 20.0%
!!! AGATAATTTTATCCGATAAA+GGG - chr2_1:77870831-77870850 None:intergenic 20.0%
!!! AAGATAATTTTATCCGATAA+AGG - chr2_1:77870832-77870851 None:intergenic 20.0%
!! TTCAATTTACAAAAACAAGA+CGG - chr2_1:77870893-77870912 None:intergenic 20.0%
!!! ATTGAAATTACTTTTTCGAT+TGG + chr2_1:77870907-77870926 Msa0227180:five_prime_UTR 20.0%
!! TAATCAAAGATGTTGAATAT+TGG + chr2_1:77870940-77870959 Msa0227180:five_prime_UTR 20.0%
!! GAAGATTTATACATCATTAT+AGG + chr2_1:77871839-77871858 Msa0227180:intron 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG + chr2_1:77871939-77871958 Msa0227180:intron 20.0%
!!! TATTTTTGTTGAAATAGAAG+GGG + chr2_1:77872859-77872878 Msa0227180:three_prime_UTR 20.0%
!!! TGTGTATTATTGATTGATAT+TGG + chr2_1:77873117-77873136 Msa0227180:three_prime_UTR 20.0%
! TACTTTAACAAACCCTTTAT+CGG + chr2_1:77870816-77870835 Msa0227180:five_prime_UTR 25.0%
! ACTTCAGTACTCTTTATATT+AGG + chr2_1:77871011-77871030 Msa0227180:five_prime_UTR 25.0%
! AGCACTAAAATGAAATGAAA+GGG - chr2_1:77871074-77871093 None:intergenic 25.0%
! TAGCACTAAAATGAAATGAA+AGG - chr2_1:77871075-77871094 None:intergenic 25.0%
! TGATATCACTTCTTACTTAT+AGG + chr2_1:77871227-77871246 Msa0227180:five_prime_UTR 25.0%
! AATAATGAAGAAGGTTTGAA+GGG - chr2_1:77871769-77871788 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG + chr2_1:77871995-77872014 Msa0227180:intron 25.0%
! TTCAACATTTCATTCTTAGT+GGG - chr2_1:77872064-77872083 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG - chr2_1:77872540-77872559 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG + chr2_1:77872542-77872561 Msa0227180:CDS 25.0%
! ACAAAACATTGTTCAAAACT+TGG + chr2_1:77872682-77872701 Msa0227180:CDS 25.0%
! ATATTAGAACTTTGCTTAAG+CGG + chr2_1:77872715-77872734 Msa0227180:CDS 25.0%
! TTACTTATTCAACCATTTCT+TGG - chr2_1:77872773-77872792 None:intergenic 25.0%
!! ATTTTTGTTGAAATAGAAGG+GGG + chr2_1:77872860-77872879 Msa0227180:three_prime_UTR 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG - chr2_1:77873050-77873069 None:intergenic 25.0%
! TGCTTTCACTTGTGTTTTAT+CGG + chr2_1:77870700-77870719 Msa0227180:five_prime_UTR 30.0%
GCATGTCAAATTTCTGTTAT+TGG + chr2_1:77871117-77871136 Msa0227180:five_prime_UTR 30.0%
TTCACATTATTCTGTCGTAA+AGG - chr2_1:77871330-77871349 None:intergenic 30.0%
!! GTTGTTGTTTATTGACAACT+TGG + chr2_1:77871402-77871421 Msa0227180:five_prime_UTR 30.0%
CAAAAATCACTCAGAACATT+GGG - chr2_1:77871451-77871470 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG + chr2_1:77871554-77871573 Msa0227180:CDS 30.0%
GAATAATGAAGAAGGTTTGA+AGG - chr2_1:77871770-77871789 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG - chr2_1:77871778-77871797 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG - chr2_1:77871929-77871948 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG - chr2_1:77871986-77872005 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG - chr2_1:77872024-77872043 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG - chr2_1:77872065-77872084 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG + chr2_1:77872292-77872311 Msa0227180:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG - chr2_1:77872388-77872407 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG + chr2_1:77872571-77872590 Msa0227180:CDS 30.0%
! GTGATTGCTAAACATTTTGA+TGG + chr2_1:77872648-77872667 Msa0227180:CDS 30.0%
TAGGTACATATATACTCTCA+TGG + chr2_1:77872744-77872763 Msa0227180:three_prime_UTR 30.0%
AAATAAAAAGATAGGGCACA+CGG - chr2_1:77872844-77872863 None:intergenic 30.0%
CATTGTCAAAAGAAAAGAAG+TGG + chr2_1:77873051-77873070 Msa0227180:three_prime_UTR 30.0%
! TCACTTGTGTTTTATCGGAT+TGG + chr2_1:77870705-77870724 Msa0227180:five_prime_UTR 35.0%
ACAATGTAAGAAAGATTGGC+AGG - chr2_1:77871283-77871302 None:intergenic 35.0%
CGAGACAATGTAAGAAAGAT+TGG - chr2_1:77871287-77871306 None:intergenic 35.0%
CCAAAAATCACTCAGAACAT+TGG - chr2_1:77871452-77871471 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG + chr2_1:77871449-77871468 Msa0227180:five_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG + chr2_1:77871455-77871474 Msa0227180:five_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG - chr2_1:77871530-77871549 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG + chr2_1:77871591-77871610 Msa0227180:CDS 35.0%
CGGATTCATCTGAAAGAATA+AGG - chr2_1:77871670-77871689 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG + chr2_1:77871779-77871798 Msa0227180:intron 35.0%
ACAAATGTCTTACAGTGCTA+TGG + chr2_1:77872117-77872136 Msa0227180:intron 35.0%
! TACAGTGCTATGGATTTTTC+AGG + chr2_1:77872127-77872146 Msa0227180:intron 35.0%
GTACATAGAAAGAATTGCTG+TGG + chr2_1:77872149-77872168 Msa0227180:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG + chr2_1:77872202-77872221 Msa0227180:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG + chr2_1:77872211-77872230 Msa0227180:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG - chr2_1:77872252-77872271 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG - chr2_1:77872290-77872309 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG + chr2_1:77872476-77872495 Msa0227180:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG - chr2_1:77872597-77872616 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG + chr2_1:77872599-77872618 Msa0227180:CDS 35.0%
GCAATCACCTTTCTATAACT+AGG - chr2_1:77872636-77872655 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG + chr2_1:77872652-77872671 Msa0227180:CDS 35.0%
! TTTATTTCTCCCTTACTGCT+TGG + chr2_1:77872890-77872909 Msa0227180:three_prime_UTR 35.0%
CACTTATCCTAGAATGAGAA+GGG + chr2_1:77872940-77872959 Msa0227180:three_prime_UTR 35.0%
AATCAAGGTATGCATGTAAG+TGG + chr2_1:77872968-77872987 Msa0227180:three_prime_UTR 35.0%
GTTTGTCTCCAGAATTTCAT+TGG + chr2_1:77872990-77873009 Msa0227180:three_prime_UTR 35.0%
TACTTGGTCCAATGAAATTC+TGG - chr2_1:77873001-77873020 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG - chr2_1:77873043-77873062 None:intergenic 35.0%
!! GTTATGTGTGACACTAGTTT+TGG + chr2_1:77873170-77873189 Msa0227180:three_prime_UTR 35.0%
TAGATCAAGAACGTAACCAA+TGG + chr2_1:77873226-77873245 Msa0227180:three_prime_UTR 35.0%
! TATAGGCTTCTGTCACGTTT+TGG + chr2_1:77871244-77871263 Msa0227180:five_prime_UTR 40.0%
ATACCTATCAGCAACTTCCT+AGG + chr2_1:77871510-77871529 Msa0227180:five_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG - chr2_1:77871567-77871586 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG + chr2_1:77871630-77871649 Msa0227180:CDS 40.0%
ATTCTTGCCAGTTACAGGAT+TGG - chr2_1:77871640-77871659 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG + chr2_1:77871641-77871660 Msa0227180:CDS 40.0%
TCCAAATTCTTGCCAGTTAC+AGG - chr2_1:77871645-77871664 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG + chr2_1:77871672-77871691 Msa0227180:CDS 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG + chr2_1:77871696-77871715 Msa0227180:CDS 40.0%
! AAATGATGTGAGAGCCGTTT+TGG - chr2_1:77871713-77871732 None:intergenic 40.0%
TATACACAATAGGCTGCTTC+AGG - chr2_1:77871976-77871995 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG + chr2_1:77872184-77872203 Msa0227180:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG + chr2_1:77872224-77872243 Msa0227180:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG - chr2_1:77872265-77872284 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG - chr2_1:77872271-77872290 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG - chr2_1:77872368-77872387 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG + chr2_1:77872409-77872428 Msa0227180:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG + chr2_1:77872446-77872465 Msa0227180:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG + chr2_1:77872480-77872499 Msa0227180:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG + chr2_1:77872610-77872629 Msa0227180:CDS 40.0%
TTTGCTTAAGCGGTACTAGT+AGG + chr2_1:77872725-77872744 Msa0227180:exon 40.0%
CTAACATCACCAAGCAGTAA+GGG - chr2_1:77872902-77872921 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG - chr2_1:77872903-77872922 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG + chr2_1:77872939-77872958 Msa0227180:three_prime_UTR 40.0%
TTATCCTAGAATGAGAAGGG+AGG + chr2_1:77872943-77872962 Msa0227180:three_prime_UTR 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG - chr2_1:77872950-77872969 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG + chr2_1:77872953-77872972 Msa0227180:three_prime_UTR 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG + chr2_1:77873013-77873032 Msa0227180:three_prime_UTR 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG + chr2_1:77873014-77873033 Msa0227180:three_prime_UTR 40.0%
TGGAACAATGCCTTCTTGTA+AGG + chr2_1:77873137-77873156 Msa0227180:three_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG - chr2_1:77873150-77873169 None:intergenic 40.0%
GGGTGAAATAAAGTAGCCAT+TGG - chr2_1:77873245-77873264 None:intergenic 40.0%
!! GTTTGATTTGGACAGCCAGA+AGG + chr2_1:77871566-77871585 Msa0227180:CDS 45.0%
! TGCCAGTTACAGGATTGGTA+TGG - chr2_1:77871635-77871654 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG - chr2_1:77871690-77871709 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG - chr2_1:77871701-77871720 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG - chr2_1:77871705-77871724 None:intergenic 45.0%
! ATAGGCTGCTTCAGGTTCAT+GGG - chr2_1:77871968-77871987 None:intergenic 45.0%
! AATAGGCTGCTTCAGGTTCA+TGG - chr2_1:77871969-77871988 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG + chr2_1:77872223-77872242 Msa0227180:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG - chr2_1:77872266-77872285 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG - chr2_1:77872272-77872291 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG - chr2_1:77872279-77872298 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG + chr2_1:77872299-77872318 Msa0227180:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG - chr2_1:77872346-77872365 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG - chr2_1:77872347-77872366 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG - chr2_1:77872348-77872367 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG - chr2_1:77872416-77872435 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG - chr2_1:77872417-77872436 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG + chr2_1:77872416-77872435 Msa0227180:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG - chr2_1:77872492-77872511 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG + chr2_1:77872592-77872611 Msa0227180:CDS 45.0%
GCAGAGGCCTAGTTATAGAA+AGG + chr2_1:77872626-77872645 Msa0227180:CDS 45.0%
CTCTCATGGTTGCCAAGAAA+TGG + chr2_1:77872758-77872777 Msa0227180:three_prime_UTR 45.0%
CCCTGAACTGTTTCAGTACT+TGG - chr2_1:77873017-77873036 None:intergenic 45.0%
!! AAAATTGATATATTAATAAA+TGG - chr2_1:77871201-77871220 None:intergenic 5.0%
CCACCTAGGAAGTTGCTGAT+AGG - chr2_1:77871516-77871535 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG + chr2_1:77871513-77871532 Msa0227180:five_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG - chr2_1:77871584-77871603 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG + chr2_1:77871581-77871600 Msa0227180:CDS 50.0%
ACTGATATCCTCAGACCCTG+CGG - chr2_1:77872178-77872197 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG - chr2_1:77872304-77872323 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG + chr2_1:77872317-77872336 Msa0227180:CDS 50.0%
AGAATTGCTGTGGTCTCCGC+AGG + chr2_1:77872159-77872178 Msa0227180:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG + chr2_1:77872160-77872179 Msa0227180:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG + chr2_1:77872336-77872355 Msa0227180:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG + chr2_1:77872425-77872444 Msa0227180:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG + chr2_1:77872497-77872516 Msa0227180:CDS 55.0%
TGTGGTCTCCGCAGGGTCTG+AGG + chr2_1:77872167-77872186 Msa0227180:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 77870639 77873268 77870639 ID=Msa0227180;Name=Msa0227180
chr2_1 mRNA 77870639 77873268 77870639 ID=Msa0227180-mRNA-1;Parent=Msa0227180;Name=Msa0227180-mRNA-1;_AED=0.01;_eAED=0.01;_QI=887|1|1|1|0.5|0.33|3|525|267
chr2_1 exon 77870639 77871365 77870639 ID=Msa0227180-mRNA-1:exon:23704;Parent=Msa0227180-mRNA-1
chr2_1 exon 77871386 77871754 77871386 ID=Msa0227180-mRNA-1:exon:23705;Parent=Msa0227180-mRNA-1
chr2_1 exon 77872149 77873268 77872149 ID=Msa0227180-mRNA-1:exon:23706;Parent=Msa0227180-mRNA-1
chr2_1 five_prime_UTR 77870639 77871365 77870639 ID=Msa0227180-mRNA-1:five_prime_utr;Parent=Msa0227180-mRNA-1
chr2_1 five_prime_UTR 77871386 77871545 77871386 ID=Msa0227180-mRNA-1:five_prime_utr;Parent=Msa0227180-mRNA-1
chr2_1 CDS 77871546 77871754 77871546 ID=Msa0227180-mRNA-1:cds;Parent=Msa0227180-mRNA-1
chr2_1 CDS 77872149 77872743 77872149 ID=Msa0227180-mRNA-1:cds;Parent=Msa0227180-mRNA-1
chr2_1 three_prime_UTR 77872744 77873268 77872744 ID=Msa0227180-mRNA-1:three_prime_utr;Parent=Msa0227180-mRNA-1
Gene Sequence

>Msa0227180

ATGCAGCTATATCATCATCCGTTTGATTTGGACAGCCAGAAGGTGAGACTTGCATTGGAAGAAAAAGGCATTGATTATACATCTTTCCATACCAATCCTGTAACTGGCAAGAATTTGGATTCCTTATTCTTTCAGATGAATCCGAGTGGAAGCCTTCCTGTTTTCCAAAACGGCTCTCACATCATTTACAAGACTATTGATATAATTCAGTACATAGAAAGAATTGCTGTGGTCTCCGCAGGGTCTGAGGATATCAGTAGCAATAGGAGTGAAGTGATTGAATGGATGAAGAGGATACAAGAGTGGGATCCTAAATATTTTTCCCTCTCCCATATACCAGAGAAACACCGAGTTTACGTTTCCAAGTTCTTAAGGCGTGTGGTGATCGCTCGTATGTCGGAGTCCCCTGAATTAGCAGGTGCATACCATAGAAAGTTGAGAGAAGCCTATCAAACTGAAGAAAAGTTGAAAGACCCTCATGTTTTGAGAAGGAGCGAGGAGCATTTGGTTAGATTGCTTGATGAAGCGGAAAGACAACTGAGTGAAACACCTTATTTGGCAGGCGAAGAGTTTAGCATGGCTGATGTGATGCTCATTCCGATTTTAGCTCGTTTAAAACTTTTGGATTTAGAGAATGAGTACATAACTATGAGGCCAAACATTGCTGAGTATTGGATTTTGGTTCAGCAGAGGCCTAGTTATAGAAAGGTGATTGCTAAACATTTTGATGGCTGGAGAAAGCACAAAACATTGTTCAAAACTTGGTGCTTTGTTCATATTAGAACTTTGCTTAAGCGGTACTAG
Protein sequence

>Msa0227180

MQLYHHPFDLDSQKVRLALEEKGIDYTSFHTNPVTGKNLDSLFFQMNPSGSLPVFQNGSHIIYKTIDIIQYIERIAVVSAGSEDISSNRSEVIEWMKRIQEWDPKYFSLSHIPEKHRVYVSKFLRRVVIARMSESPELAGAYHRKLREAYQTEEKLKDPHVLRRSEEHLVRLLDEAERQLSETPYLAGEEFSMADVMLIPILARLKLLDLENEYITMRPNIAEYWILVQQRPSYRKVIAKHFDGWRKHKTLFKTWCFVHIRTLLKRY*