AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0423570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423570 MtrunA17_Chr3g0125261 98.502 267 4 0 1 267 1 267 0.0 547
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423570 AT1G77290.3 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa0423570 AT1G77290.1 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa0423570 AT1G77290.2 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa0423570 AT4G02520.1 24.280 243 143 4 1 237 4 211 7.43e-14 69.3

Find 85 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGCTGAGTATTGGATTT+TGG 0.152263 3_2:-73503860 Msa0423570:CDS
TCCTGTAACTGGCAAGAATT+TGG 0.171996 3_2:-73504818 Msa0423570:CDS
TTTAGCTCGTTTAAAACTTT+TGG 0.180573 3_2:-73503917 Msa0423570:CDS
ACTGAGTGAAACACCTTATT+TGG 0.190936 3_2:-73503983 Msa0423570:CDS
CTCGGTGTTTCTCTGGTATA+TGG 0.211375 3_2:+73504190 None:intergenic
TCCAAATTCTTGCCAGTTAC+AGG 0.236554 3_2:+73504817 None:intergenic
AACTCTTCGCCTGCCAAATA+AGG 0.264517 3_2:+73503970 None:intergenic
TTACTTATTCAACCATTTCT+TGG 0.265920 3_2:+73503689 None:intergenic
AGGCTTCTCTCAACTTTCTA+TGG 0.276841 3_2:+73504094 None:intergenic
TGGTATGCACCTGCTAATTC+AGG 0.285480 3_2:+73504114 None:intergenic
ATATCATCATCCGTTTGATT+TGG 0.293342 3_2:-73504905 Msa0423570:CDS
CGGATTCATCTGAAAGAATA+AGG 0.297726 3_2:+73504792 None:intergenic
CCCTGAACTGTTTCAGTACT+TGG 0.317517 3_2:+73503445 None:intergenic
TACTTGGTCCAATGAAATTC+TGG 0.327240 3_2:+73503461 None:intergenic
TTTACGTTTCCAAGTTCTTA+AGG 0.342669 3_2:-73504167 Msa0423570:CDS
CCAATGCAAGTCTCACCTTC+TGG 0.346297 3_2:+73504878 None:intergenic
TGTGTATTATTGATTGATAT+TGG 0.347592 3_2:-73503342 Msa0423570:three_prime_UTR
AAATGATGTGAGAGCCGTTT+TGG 0.353112 3_2:+73504749 None:intergenic
AGACTTGCATTGGAAGAAAA+AGG 0.354375 3_2:-73504868 Msa0423570:CDS
GTTATGTGTGACACTAGTTT+TGG 0.361960 3_2:-73503289 Msa0423570:three_prime_UTR
TGGAACAATGCCTTCTTGTA+AGG 0.374184 3_2:-73503322 Msa0423570:three_prime_UTR
TTCCATACCAATCCTGTAAC+TGG 0.385351 3_2:-73504829 Msa0423570:CDS
GAGAAGGAGCGAGGAGCATT+TGG 0.386161 3_2:-73504034 Msa0423570:CDS
GTTTGTCTCCAGAATTTCAT+TGG 0.390685 3_2:-73503469 Msa0423570:three_prime_UTR
CACCACACGCCTTAAGAACT+TGG 0.391896 3_2:+73504158 None:intergenic
TTTATTTCTCCCTTACTGCT+TGG 0.399542 3_2:-73503569 Msa0423570:three_prime_UTR
ACCAAGTACTGAAACAGTTC+AGG 0.403387 3_2:-73503446 Msa0423570:three_prime_UTR
CTCTCATGGTTGCCAAGAAA+TGG 0.406293 3_2:-73503701 Msa0423570:three_prime_UTR
ATCCAATACTCAGCAATGTT+TGG 0.413409 3_2:+73503865 None:intergenic
ACGTAAACTCGGTGTTTCTC+TGG 0.433917 3_2:+73504183 None:intergenic
GGCCAAACATTGCTGAGTAT+TGG 0.435036 3_2:-73503867 Msa0423570:CDS
CCACCTAGGAAGTTGCTGAT+AGG 0.448934 3_2:+73504946 None:intergenic
AGAATTGCTGTGGTCTCCGC+AGG 0.450946 3_2:-73504300 Msa0423570:CDS
CTGAGGATATCAGTAGCAAT+AGG 0.470164 3_2:-73504275 Msa0423570:CDS
ATTCTTGCCAGTTACAGGAT+TGG 0.485946 3_2:+73504822 None:intergenic
ACTAACATCACCAAGCAGTA+AGG 0.489289 3_2:+73503559 None:intergenic
GTTGTTGTTTATTGACAACT+TGG 0.490653 3_2:-73505057 Msa0423570:five_prime_UTR
TTCTGGCTGTCCAAATCAAA+CGG 0.493106 3_2:+73504895 None:intergenic
TTTGCTTAAGCGGTACTAGT+AGG 0.493567 3_2:-73503734 Msa0423570:exon
ACAAAACATTGTTCAAAACT+TGG 0.494072 3_2:-73503777 Msa0423570:CDS
TGCCAGTTACAGGATTGGTA+TGG 0.497143 3_2:+73504827 None:intergenic
GCAATCACCTTTCTATAACT+AGG 0.510277 3_2:+73503826 None:intergenic
ATATTAGAACTTTGCTTAAG+CGG 0.513812 3_2:-73503744 Msa0423570:CDS
GGTTAGATTGCTTGATGAAG+CGG 0.515391 3_2:-73504013 Msa0423570:CDS
ATACCTATCAGCAACTTCCT+AGG 0.523035 3_2:-73504949 Msa0423570:five_prime_UTR
GGTATGCACCTGCTAATTCA+GGG 0.526259 3_2:+73504115 None:intergenic
TCGGTGTTTCTCTGGTATAT+GGG 0.528301 3_2:+73504191 None:intergenic
ATGAGAAGGGAGGAAAATCA+AGG 0.529179 3_2:-73503506 Msa0423570:three_prime_UTR
GGATGAAGAGGATACAAGAG+TGG 0.530840 3_2:-73504236 Msa0423570:CDS
AGTGAAACACCTTATTTGGC+AGG 0.533423 3_2:-73503979 Msa0423570:CDS
TGTGGTGATCGCTCGTATGT+CGG 0.536691 3_2:-73504142 Msa0423570:CDS
TCGGAGTCCCCTGAATTAGC+AGG 0.546117 3_2:-73504123 Msa0423570:CDS
TGTGGTCTCCGCAGGGTCTG+AGG 0.546519 3_2:-73504292 Msa0423570:CDS
CCAGAAGGTGAGACTTGCAT+TGG 0.548807 3_2:-73504878 Msa0423570:CDS
GCAGAGGCCTAGTTATAGAA+AGG 0.552401 3_2:-73503833 Msa0423570:CDS
ATAGGAGTGAAGTGATTGAA+TGG 0.553982 3_2:-73504257 Msa0423570:CDS
AATCAAGGTATGCATGTAAG+TGG 0.555188 3_2:-73503491 Msa0423570:three_prime_UTR
TTCCAAGTTCTTAAGGCGTG+TGG 0.557777 3_2:-73504160 Msa0423570:CDS
GTTTCTCTGGTATATGGGAG+AGG 0.560412 3_2:+73504196 None:intergenic
CACTTATCCTAGAATGAGAA+GGG 0.565199 3_2:-73503519 Msa0423570:three_prime_UTR
CTAACATCACCAAGCAGTAA+GGG 0.567559 3_2:+73503560 None:intergenic
CATTGTCAAAAGAAAAGAAG+TGG 0.576035 3_2:-73503408 Msa0423570:three_prime_UTR
GGGTGAAATAAAGTAGCCAT+TGG 0.581067 3_2:+73503217 None:intergenic
AGAATGAGTACATAACTATG+AGG 0.584947 3_2:-73503888 Msa0423570:CDS
CGCTCCTTCTCAAAACATGA+GGG 0.586987 3_2:+73504046 None:intergenic
CCAAGTACTGAAACAGTTCA+GGG 0.589412 3_2:-73503445 Msa0423570:three_prime_UTR
GCACTTATCCTAGAATGAGA+AGG 0.590458 3_2:-73503520 Msa0423570:three_prime_UTR
TCGCTCCTTCTCAAAACATG+AGG 0.593199 3_2:+73504045 None:intergenic
AAGCAGCAATCCTTACAAGA+AGG 0.594753 3_2:+73503312 None:intergenic
GGCAGGCGAAGAGTTTAGCA+TGG 0.595558 3_2:-73503962 Msa0423570:CDS
GTTTGATTTGGACAGCCAGA+AGG 0.595603 3_2:-73504893 Msa0423570:CDS
TAGGTACATATATACTCTCA+TGG 0.604593 3_2:-73503715 Msa0423570:three_prime_UTR
AAAACAGGAAGGCTTCCACT+CGG 0.612067 3_2:+73504772 None:intergenic
TTTCTCTGGTATATGGGAGA+GGG 0.623180 3_2:+73504197 None:intergenic
GATGAAGAGGATACAAGAGT+GGG 0.626925 3_2:-73504235 Msa0423570:CDS
AGAACTTGGAAACGTAAACT+CGG 0.634065 3_2:+73504172 None:intergenic
GTACATAGAAAGAATTGCTG+TGG 0.635061 3_2:-73504310 Msa0423570:CDS
TAGATCAAGAACGTAACCAA+TGG 0.635563 3_2:-73503233 Msa0423570:three_prime_UTR
AAGTGATTGAATGGATGAAG+AGG 0.651845 3_2:-73504248 Msa0423570:CDS
TTCTTTCAGATGAATCCGAG+TGG 0.664252 3_2:-73504787 Msa0423570:CDS
GAATTGCTGTGGTCTCCGCA+GGG 0.673553 3_2:-73504299 Msa0423570:CDS
GTATGCACCTGCTAATTCAG+GGG 0.678849 3_2:+73504116 None:intergenic
TTATCCTAGAATGAGAAGGG+AGG 0.687206 3_2:-73503516 Msa0423570:three_prime_UTR
CCTATCAGCAACTTCCTAGG+TGG 0.692964 3_2:-73504946 Msa0423570:five_prime_UTR
ACTGATATCCTCAGACCCTG+CGG 0.699635 3_2:+73504284 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATCTTTAAACTACTATA+TGG + chr3_2:73503695-73503714 None:intergenic 15.0%
!! TCAACAAAAATAAAAAGATA+GGG + chr3_2:73504677-73504696 None:intergenic 15.0%
!! TTCAACAAAAATAAAAAGAT+AGG + chr3_2:73504678-73504697 None:intergenic 15.0%
!!! TTTATTTTTGTTGAAATAGA+AGG - chr3_2:73504683-73504702 Msa0423570:intron 15.0%
!!! TTATTTTTGTTGAAATAGAA+GGG - chr3_2:73504684-73504703 Msa0423570:intron 15.0%
!! TTTATCTTTAAACTACTATA+TGG + chr3_2:73503695-73503714 None:intergenic 15.0%
!! TCAACAAAAATAAAAAGATA+GGG + chr3_2:73504677-73504696 None:intergenic 15.0%
!! TTCAACAAAAATAAAAAGAT+AGG + chr3_2:73504678-73504697 None:intergenic 15.0%
!!! TTTATTTTTGTTGAAATAGA+AGG - chr3_2:73504683-73504702 Msa0423570:intron 15.0%
!!! TTATTTTTGTTGAAATAGAA+GGG - chr3_2:73504684-73504703 Msa0423570:intron 15.0%
!! GAAGATTTATACATCATTAT+AGG - chr3_2:73503665-73503684 Msa0423570:three_prime_UTR 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG - chr3_2:73503765-73503784 Msa0423570:CDS 20.0%
!!! TATTTTTGTTGAAATAGAAG+GGG - chr3_2:73504685-73504704 Msa0423570:intron 20.0%
!!! TGTGTATTATTGATTGATAT+TGG - chr3_2:73504943-73504962 Msa0423570:five_prime_UTR 20.0%
!! GAAGATTTATACATCATTAT+AGG - chr3_2:73503665-73503684 Msa0423570:three_prime_UTR 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG - chr3_2:73503765-73503784 Msa0423570:CDS 20.0%
!!! TATTTTTGTTGAAATAGAAG+GGG - chr3_2:73504685-73504704 Msa0423570:intron 20.0%
!!! TGTGTATTATTGATTGATAT+TGG - chr3_2:73504943-73504962 Msa0423570:five_prime_UTR 20.0%
! AATAATGAAGAAGGTTTGAA+GGG + chr3_2:73503595-73503614 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG - chr3_2:73503821-73503840 Msa0423570:CDS 25.0%
! TTCAACATTTCATTCTTAGT+GGG + chr3_2:73503890-73503909 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG + chr3_2:73504366-73504385 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG - chr3_2:73504368-73504387 Msa0423570:intron 25.0%
! ACAAAACATTGTTCAAAACT+TGG - chr3_2:73504508-73504527 Msa0423570:intron 25.0%
! ATATTAGAACTTTGCTTAAG+CGG - chr3_2:73504541-73504560 Msa0423570:intron 25.0%
! TTACTTATTCAACCATTTCT+TGG + chr3_2:73504599-73504618 None:intergenic 25.0%
!! ATTTTTGTTGAAATAGAAGG+GGG - chr3_2:73504686-73504705 Msa0423570:intron 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG + chr3_2:73504876-73504895 None:intergenic 25.0%
! AATAATGAAGAAGGTTTGAA+GGG + chr3_2:73503595-73503614 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG - chr3_2:73503821-73503840 Msa0423570:CDS 25.0%
! TTCAACATTTCATTCTTAGT+GGG + chr3_2:73503890-73503909 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG + chr3_2:73504366-73504385 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG - chr3_2:73504368-73504387 Msa0423570:intron 25.0%
! ACAAAACATTGTTCAAAACT+TGG - chr3_2:73504508-73504527 Msa0423570:intron 25.0%
! ATATTAGAACTTTGCTTAAG+CGG - chr3_2:73504541-73504560 Msa0423570:intron 25.0%
! TTACTTATTCAACCATTTCT+TGG + chr3_2:73504599-73504618 None:intergenic 25.0%
!! ATTTTTGTTGAAATAGAAGG+GGG - chr3_2:73504686-73504705 Msa0423570:intron 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG + chr3_2:73504876-73504895 None:intergenic 25.0%
!! GTTGTTGTTTATTGACAACT+TGG - chr3_2:73503228-73503247 Msa0423570:three_prime_UTR 30.0%
CAAAAATCACTCAGAACATT+GGG + chr3_2:73503277-73503296 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG - chr3_2:73503380-73503399 Msa0423570:three_prime_UTR 30.0%
GAATAATGAAGAAGGTTTGA+AGG + chr3_2:73503596-73503615 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG + chr3_2:73503604-73503623 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG + chr3_2:73503755-73503774 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG + chr3_2:73503812-73503831 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG + chr3_2:73503850-73503869 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG + chr3_2:73503891-73503910 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG - chr3_2:73504118-73504137 Msa0423570:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG + chr3_2:73504214-73504233 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG - chr3_2:73504397-73504416 Msa0423570:intron 30.0%
! GTGATTGCTAAACATTTTGA+TGG - chr3_2:73504474-73504493 Msa0423570:intron 30.0%
TAGGTACATATATACTCTCA+TGG - chr3_2:73504570-73504589 Msa0423570:intron 30.0%
AAATAAAAAGATAGGGCACA+CGG + chr3_2:73504670-73504689 None:intergenic 30.0%
CATTGTCAAAAGAAAAGAAG+TGG - chr3_2:73504877-73504896 Msa0423570:CDS 30.0%
!! GTTGTTGTTTATTGACAACT+TGG - chr3_2:73503228-73503247 Msa0423570:three_prime_UTR 30.0%
CAAAAATCACTCAGAACATT+GGG + chr3_2:73503277-73503296 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG - chr3_2:73503380-73503399 Msa0423570:three_prime_UTR 30.0%
GAATAATGAAGAAGGTTTGA+AGG + chr3_2:73503596-73503615 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG + chr3_2:73503604-73503623 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG + chr3_2:73503755-73503774 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG + chr3_2:73503812-73503831 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG + chr3_2:73503850-73503869 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG + chr3_2:73503891-73503910 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG - chr3_2:73504118-73504137 Msa0423570:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG + chr3_2:73504214-73504233 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG - chr3_2:73504397-73504416 Msa0423570:intron 30.0%
! GTGATTGCTAAACATTTTGA+TGG - chr3_2:73504474-73504493 Msa0423570:intron 30.0%
TAGGTACATATATACTCTCA+TGG - chr3_2:73504570-73504589 Msa0423570:intron 30.0%
AAATAAAAAGATAGGGCACA+CGG + chr3_2:73504670-73504689 None:intergenic 30.0%
CATTGTCAAAAGAAAAGAAG+TGG - chr3_2:73504877-73504896 Msa0423570:CDS 30.0%
CCAAAAATCACTCAGAACAT+TGG + chr3_2:73503278-73503297 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG - chr3_2:73503275-73503294 Msa0423570:three_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG - chr3_2:73503281-73503300 Msa0423570:three_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG + chr3_2:73503356-73503375 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG - chr3_2:73503417-73503436 Msa0423570:three_prime_UTR 35.0%
CGGATTCATCTGAAAGAATA+AGG + chr3_2:73503496-73503515 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG - chr3_2:73503605-73503624 Msa0423570:three_prime_UTR 35.0%
ACAAATGTCTTACAGTGCTA+TGG - chr3_2:73503943-73503962 Msa0423570:CDS 35.0%
! TACAGTGCTATGGATTTTTC+AGG - chr3_2:73503953-73503972 Msa0423570:CDS 35.0%
GTACATAGAAAGAATTGCTG+TGG - chr3_2:73503975-73503994 Msa0423570:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG - chr3_2:73504028-73504047 Msa0423570:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG - chr3_2:73504037-73504056 Msa0423570:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG + chr3_2:73504078-73504097 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG + chr3_2:73504116-73504135 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG - chr3_2:73504302-73504321 Msa0423570:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG + chr3_2:73504423-73504442 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG - chr3_2:73504425-73504444 Msa0423570:intron 35.0%
GCAATCACCTTTCTATAACT+AGG + chr3_2:73504462-73504481 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG - chr3_2:73504478-73504497 Msa0423570:intron 35.0%
! TTTATTTCTCCCTTACTGCT+TGG - chr3_2:73504716-73504735 Msa0423570:intron 35.0%
CACTTATCCTAGAATGAGAA+GGG - chr3_2:73504766-73504785 Msa0423570:CDS 35.0%
AATCAAGGTATGCATGTAAG+TGG - chr3_2:73504794-73504813 Msa0423570:CDS 35.0%
GTTTGTCTCCAGAATTTCAT+TGG - chr3_2:73504816-73504835 Msa0423570:CDS 35.0%
TACTTGGTCCAATGAAATTC+TGG + chr3_2:73504827-73504846 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG + chr3_2:73504869-73504888 None:intergenic 35.0%
!! GTTATGTGTGACACTAGTTT+TGG - chr3_2:73504996-73505015 Msa0423570:five_prime_UTR 35.0%
TAGATCAAGAACGTAACCAA+TGG - chr3_2:73505052-73505071 Msa0423570:five_prime_UTR 35.0%
CCAAAAATCACTCAGAACAT+TGG + chr3_2:73503278-73503297 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG - chr3_2:73503275-73503294 Msa0423570:three_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG - chr3_2:73503281-73503300 Msa0423570:three_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG + chr3_2:73503356-73503375 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG - chr3_2:73503417-73503436 Msa0423570:three_prime_UTR 35.0%
CGGATTCATCTGAAAGAATA+AGG + chr3_2:73503496-73503515 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG - chr3_2:73503605-73503624 Msa0423570:three_prime_UTR 35.0%
ACAAATGTCTTACAGTGCTA+TGG - chr3_2:73503943-73503962 Msa0423570:CDS 35.0%
! TACAGTGCTATGGATTTTTC+AGG - chr3_2:73503953-73503972 Msa0423570:CDS 35.0%
GTACATAGAAAGAATTGCTG+TGG - chr3_2:73503975-73503994 Msa0423570:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG - chr3_2:73504028-73504047 Msa0423570:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG - chr3_2:73504037-73504056 Msa0423570:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG + chr3_2:73504078-73504097 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG + chr3_2:73504116-73504135 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG - chr3_2:73504302-73504321 Msa0423570:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG + chr3_2:73504423-73504442 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG - chr3_2:73504425-73504444 Msa0423570:intron 35.0%
GCAATCACCTTTCTATAACT+AGG + chr3_2:73504462-73504481 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG - chr3_2:73504478-73504497 Msa0423570:intron 35.0%
! TTTATTTCTCCCTTACTGCT+TGG - chr3_2:73504716-73504735 Msa0423570:intron 35.0%
CACTTATCCTAGAATGAGAA+GGG - chr3_2:73504766-73504785 Msa0423570:CDS 35.0%
AATCAAGGTATGCATGTAAG+TGG - chr3_2:73504794-73504813 Msa0423570:CDS 35.0%
GTTTGTCTCCAGAATTTCAT+TGG - chr3_2:73504816-73504835 Msa0423570:CDS 35.0%
TACTTGGTCCAATGAAATTC+TGG + chr3_2:73504827-73504846 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG + chr3_2:73504869-73504888 None:intergenic 35.0%
!! GTTATGTGTGACACTAGTTT+TGG - chr3_2:73504996-73505015 Msa0423570:five_prime_UTR 35.0%
TAGATCAAGAACGTAACCAA+TGG - chr3_2:73505052-73505071 Msa0423570:five_prime_UTR 35.0%
ATACCTATCAGCAACTTCCT+AGG - chr3_2:73503336-73503355 Msa0423570:three_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG + chr3_2:73503393-73503412 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG - chr3_2:73503456-73503475 Msa0423570:three_prime_UTR 40.0%
ATTCTTGCCAGTTACAGGAT+TGG + chr3_2:73503466-73503485 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG - chr3_2:73503467-73503486 Msa0423570:three_prime_UTR 40.0%
TCCAAATTCTTGCCAGTTAC+AGG + chr3_2:73503471-73503490 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG - chr3_2:73503498-73503517 Msa0423570:three_prime_UTR 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG - chr3_2:73503522-73503541 Msa0423570:three_prime_UTR 40.0%
! AAATGATGTGAGAGCCGTTT+TGG + chr3_2:73503539-73503558 None:intergenic 40.0%
TATACACAATAGGCTGCTTC+AGG + chr3_2:73503802-73503821 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG - chr3_2:73504010-73504029 Msa0423570:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG - chr3_2:73504050-73504069 Msa0423570:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG + chr3_2:73504091-73504110 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG + chr3_2:73504097-73504116 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG + chr3_2:73504194-73504213 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG - chr3_2:73504235-73504254 Msa0423570:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG - chr3_2:73504272-73504291 Msa0423570:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG - chr3_2:73504306-73504325 Msa0423570:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG - chr3_2:73504436-73504455 Msa0423570:intron 40.0%
TTTGCTTAAGCGGTACTAGT+AGG - chr3_2:73504551-73504570 Msa0423570:intron 40.0%
CTAACATCACCAAGCAGTAA+GGG + chr3_2:73504728-73504747 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG + chr3_2:73504729-73504748 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG - chr3_2:73504765-73504784 Msa0423570:CDS 40.0%
TTATCCTAGAATGAGAAGGG+AGG - chr3_2:73504769-73504788 Msa0423570:CDS 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG + chr3_2:73504776-73504795 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG - chr3_2:73504779-73504798 Msa0423570:CDS 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG - chr3_2:73504839-73504858 Msa0423570:CDS 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG - chr3_2:73504840-73504859 Msa0423570:CDS 40.0%
TGGAACAATGCCTTCTTGTA+AGG - chr3_2:73504963-73504982 Msa0423570:five_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG + chr3_2:73504976-73504995 None:intergenic 40.0%
ATACCTATCAGCAACTTCCT+AGG - chr3_2:73503336-73503355 Msa0423570:three_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG + chr3_2:73503393-73503412 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG - chr3_2:73503456-73503475 Msa0423570:three_prime_UTR 40.0%
ATTCTTGCCAGTTACAGGAT+TGG + chr3_2:73503466-73503485 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG - chr3_2:73503467-73503486 Msa0423570:three_prime_UTR 40.0%
TCCAAATTCTTGCCAGTTAC+AGG + chr3_2:73503471-73503490 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG - chr3_2:73503498-73503517 Msa0423570:three_prime_UTR 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG - chr3_2:73503522-73503541 Msa0423570:three_prime_UTR 40.0%
! AAATGATGTGAGAGCCGTTT+TGG + chr3_2:73503539-73503558 None:intergenic 40.0%
TATACACAATAGGCTGCTTC+AGG + chr3_2:73503802-73503821 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG - chr3_2:73504010-73504029 Msa0423570:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG - chr3_2:73504050-73504069 Msa0423570:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG + chr3_2:73504091-73504110 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG + chr3_2:73504097-73504116 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG + chr3_2:73504194-73504213 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG - chr3_2:73504235-73504254 Msa0423570:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG - chr3_2:73504272-73504291 Msa0423570:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG - chr3_2:73504306-73504325 Msa0423570:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG - chr3_2:73504436-73504455 Msa0423570:intron 40.0%
TTTGCTTAAGCGGTACTAGT+AGG - chr3_2:73504551-73504570 Msa0423570:intron 40.0%
CTAACATCACCAAGCAGTAA+GGG + chr3_2:73504728-73504747 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG + chr3_2:73504729-73504748 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG - chr3_2:73504765-73504784 Msa0423570:CDS 40.0%
TTATCCTAGAATGAGAAGGG+AGG - chr3_2:73504769-73504788 Msa0423570:CDS 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG + chr3_2:73504776-73504795 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG - chr3_2:73504779-73504798 Msa0423570:CDS 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG - chr3_2:73504839-73504858 Msa0423570:CDS 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG - chr3_2:73504840-73504859 Msa0423570:CDS 40.0%
TGGAACAATGCCTTCTTGTA+AGG - chr3_2:73504963-73504982 Msa0423570:five_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG + chr3_2:73504976-73504995 None:intergenic 40.0%
!! GTTTGATTTGGACAGCCAGA+AGG - chr3_2:73503392-73503411 Msa0423570:three_prime_UTR 45.0%
! TGCCAGTTACAGGATTGGTA+TGG + chr3_2:73503461-73503480 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG + chr3_2:73503516-73503535 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG + chr3_2:73503527-73503546 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG + chr3_2:73503531-73503550 None:intergenic 45.0%
! ATAGGCTGCTTCAGGTTCAT+GGG + chr3_2:73503794-73503813 None:intergenic 45.0%
! AATAGGCTGCTTCAGGTTCA+TGG + chr3_2:73503795-73503814 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG - chr3_2:73504049-73504068 Msa0423570:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG + chr3_2:73504092-73504111 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG + chr3_2:73504098-73504117 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG + chr3_2:73504105-73504124 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG - chr3_2:73504125-73504144 Msa0423570:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG + chr3_2:73504172-73504191 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG + chr3_2:73504173-73504192 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG + chr3_2:73504174-73504193 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG + chr3_2:73504242-73504261 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG + chr3_2:73504243-73504262 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG - chr3_2:73504242-73504261 Msa0423570:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG + chr3_2:73504318-73504337 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG - chr3_2:73504418-73504437 Msa0423570:intron 45.0%
GCAGAGGCCTAGTTATAGAA+AGG - chr3_2:73504452-73504471 Msa0423570:intron 45.0%
CTCTCATGGTTGCCAAGAAA+TGG - chr3_2:73504584-73504603 Msa0423570:intron 45.0%
CCCTGAACTGTTTCAGTACT+TGG + chr3_2:73504843-73504862 None:intergenic 45.0%
!! GTTTGATTTGGACAGCCAGA+AGG - chr3_2:73503392-73503411 Msa0423570:three_prime_UTR 45.0%
! TGCCAGTTACAGGATTGGTA+TGG + chr3_2:73503461-73503480 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG + chr3_2:73503516-73503535 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG + chr3_2:73503527-73503546 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG + chr3_2:73503531-73503550 None:intergenic 45.0%
! ATAGGCTGCTTCAGGTTCAT+GGG + chr3_2:73503794-73503813 None:intergenic 45.0%
! AATAGGCTGCTTCAGGTTCA+TGG + chr3_2:73503795-73503814 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG - chr3_2:73504049-73504068 Msa0423570:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG + chr3_2:73504092-73504111 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG + chr3_2:73504098-73504117 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG + chr3_2:73504105-73504124 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG - chr3_2:73504125-73504144 Msa0423570:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG + chr3_2:73504172-73504191 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG + chr3_2:73504173-73504192 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG + chr3_2:73504174-73504193 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG + chr3_2:73504242-73504261 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG + chr3_2:73504243-73504262 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG - chr3_2:73504242-73504261 Msa0423570:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG + chr3_2:73504318-73504337 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG - chr3_2:73504418-73504437 Msa0423570:intron 45.0%
GCAGAGGCCTAGTTATAGAA+AGG - chr3_2:73504452-73504471 Msa0423570:intron 45.0%
CTCTCATGGTTGCCAAGAAA+TGG - chr3_2:73504584-73504603 Msa0423570:intron 45.0%
CCCTGAACTGTTTCAGTACT+TGG + chr3_2:73504843-73504862 None:intergenic 45.0%
CCACCTAGGAAGTTGCTGAT+AGG + chr3_2:73503342-73503361 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG - chr3_2:73503339-73503358 Msa0423570:three_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG + chr3_2:73503410-73503429 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG - chr3_2:73503407-73503426 Msa0423570:three_prime_UTR 50.0%
ACTGATATCCTCAGACCCTG+CGG + chr3_2:73504004-73504023 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG + chr3_2:73504130-73504149 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG - chr3_2:73504143-73504162 Msa0423570:CDS 50.0%
CCACCTAGGAAGTTGCTGAT+AGG + chr3_2:73503342-73503361 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG - chr3_2:73503339-73503358 Msa0423570:three_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG + chr3_2:73503410-73503429 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG - chr3_2:73503407-73503426 Msa0423570:three_prime_UTR 50.0%
ACTGATATCCTCAGACCCTG+CGG + chr3_2:73504004-73504023 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG + chr3_2:73504130-73504149 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG - chr3_2:73504143-73504162 Msa0423570:CDS 50.0%
AGAATTGCTGTGGTCTCCGC+AGG - chr3_2:73503985-73504004 Msa0423570:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG - chr3_2:73503986-73504005 Msa0423570:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG - chr3_2:73504162-73504181 Msa0423570:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG - chr3_2:73504251-73504270 Msa0423570:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG - chr3_2:73504323-73504342 Msa0423570:intron 55.0%
AGAATTGCTGTGGTCTCCGC+AGG - chr3_2:73503985-73504004 Msa0423570:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG - chr3_2:73503986-73504005 Msa0423570:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG - chr3_2:73504162-73504181 Msa0423570:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG - chr3_2:73504251-73504270 Msa0423570:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG - chr3_2:73504323-73504342 Msa0423570:intron 55.0%
TGTGGTCTCCGCAGGGTCTG+AGG - chr3_2:73503993-73504012 Msa0423570:CDS 65.0%
TGTGGTCTCCGCAGGGTCTG+AGG - chr3_2:73503993-73504012 Msa0423570:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 73503222 73505085 73503222 ID=Msa0423570;Name=Msa0423570
chr3_2 mRNA 73503222 73505085 73503222 ID=Msa0423570-mRNA-1;Parent=Msa0423570;Name=Msa0423570-mRNA-1;_AED=0.01;_eAED=0.01;_QI=150|1|1|1|1|1|2|516|267
chr3_2 exon 73503222 73504332 73503222 ID=Msa0423570-mRNA-1:exon:18364;Parent=Msa0423570-mRNA-1
chr3_2 exon 73504727 73505085 73504727 ID=Msa0423570-mRNA-1:exon:18363;Parent=Msa0423570-mRNA-1
chr3_2 five_prime_UTR 73504936 73505085 73504936 ID=Msa0423570-mRNA-1:five_prime_utr;Parent=Msa0423570-mRNA-1
chr3_2 CDS 73504727 73504935 73504727 ID=Msa0423570-mRNA-1:cds;Parent=Msa0423570-mRNA-1
chr3_2 CDS 73503738 73504332 73503738 ID=Msa0423570-mRNA-1:cds;Parent=Msa0423570-mRNA-1
chr3_2 three_prime_UTR 73503222 73503737 73503222 ID=Msa0423570-mRNA-1:three_prime_utr;Parent=Msa0423570-mRNA-1
Gene Sequence

>Msa0423570

ATGCAGCTATATCATCATCCGTTTGATTTGGACAGCCAGAAGGTGAGACTTGCATTGGAAGAAAAAGGCATTGATTATACATCTTTCCATACCAATCCTGTAACTGGCAAGAATTTGGATTCCTTATTCTTTCAGATGAATCCGAGTGGAAGCCTTCCTGTTTTCCAAAACGGCTCTCACATCATTTACAAGACTATTGATATAATTCAGTACATAGAAAGAATTGCTGTGGTCTCCGCAGGGTCTGAGGATATCAGTAGCAATAGGAGTGAAGTGATTGAATGGATGAAGAGGATACAAGAGTGGGATCCTAAATATTTTTCCCTCTCCCATATACCAGAGAAACACCGAGTTTACGTTTCCAAGTTCTTAAGGCGTGTGGTGATCGCTCGTATGTCGGAGTCCCCTGAATTAGCAGGTGCATACCATAGAAAGTTGAGAGAAGCCTATCAAACTGAAGAAAAGTTGAAAGACCCTCATGTTTTGAGAAGGAGCGAGGAGCATTTGGTTAGATTGCTTGATGAAGCGGAAAGACAACTGAGTGAAACACCTTATTTGGCAGGCGAAGAGTTTAGCATGGCTGATGTGATGCTCATTCCGATTTTAGCTCGTTTAAAACTTTTGGATTTAGAGAATGAGTACATAACTATGAGGCCAAACATTGCTGAGTATTGGATTTTGGTTCAGCAGAGGCCTAGTTATAGAAAGGTGATTGCTAAACATTTTGATGGCTGGAGAAAGCACAAAACATTGTTCAAAACTTGGTGCTTTGTTCATATTAGAACTTTGCTTAAGCGGTACTAG
Protein sequence

>Msa0423570

MQLYHHPFDLDSQKVRLALEEKGIDYTSFHTNPVTGKNLDSLFFQMNPSGSLPVFQNGSHIIYKTIDIIQYIERIAVVSAGSEDISSNRSEVIEWMKRIQEWDPKYFSLSHIPEKHRVYVSKFLRRVVIARMSESPELAGAYHRKLREAYQTEEKLKDPHVLRRSEEHLVRLLDEAERQLSETPYLAGEEFSMADVMLIPILARLKLLDLENEYITMRPNIAEYWILVQQRPSYRKVIAKHFDGWRKHKTLFKTWCFVHIRTLLKRY*