AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1380470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380470 MtrunA17_Chr3g0125261 98.502 267 4 0 1 267 1 267 0.0 547
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380470 AT1G77290.3 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa1380470 AT1G77290.1 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa1380470 AT1G77290.2 68.914 267 82 1 1 267 1 266 2.69e-141 398
Msa1380470 AT4G02520.1 24.280 243 143 4 1 237 4 211 7.43e-14 69.3

Find 86 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGCTGAGTATTGGATTT+TGG 0.152263 tig0020363:+21650 Msa1380470:CDS
TCCTGTAACTGGCAAGAATT+TGG 0.171996 tig0020363:+20692 Msa1380470:CDS
TTTAGCTCGTTTAAAACTTT+TGG 0.180573 tig0020363:+21593 Msa1380470:CDS
ACTGAGTGAAACACCTTATT+TGG 0.190936 tig0020363:+21527 Msa1380470:CDS
CTCGGTGTTTCTCTGGTATA+TGG 0.211375 tig0020363:-21320 None:intergenic
TCCAAATTCTTGCCAGTTAC+AGG 0.236554 tig0020363:-20693 None:intergenic
AACTCTTCGCCTGCCAAATA+AGG 0.264517 tig0020363:-21540 None:intergenic
TTACTTATTCAACCATTTCT+TGG 0.265920 tig0020363:-21821 None:intergenic
AGGCTTCTCTCAACTTTCTA+TGG 0.276841 tig0020363:-21416 None:intergenic
TGGTATGCACCTGCTAATTC+AGG 0.285480 tig0020363:-21396 None:intergenic
ATATCATCATCCGTTTGATT+TGG 0.293342 tig0020363:+20605 Msa1380470:CDS
CGGATTCATCTGAAAGAATA+AGG 0.297726 tig0020363:-20718 None:intergenic
AAGTGGAGAGAGTGAGAAAA+AGG 0.302361 tig0020363:-19606 None:intergenic
CCCTGAACTGTTTCAGTACT+TGG 0.317517 tig0020363:-22063 None:intergenic
TACTTGGTCCAATGAAATTC+TGG 0.327240 tig0020363:-22047 None:intergenic
TTTACGTTTCCAAGTTCTTA+AGG 0.342669 tig0020363:+21343 Msa1380470:CDS
CCAATGCAAGTCTCACCTTC+TGG 0.346297 tig0020363:-20632 None:intergenic
TGTGTATTATTGATTGATAT+TGG 0.347592 tig0020363:+22166 Msa1380470:three_prime_UTR
AAATGATGTGAGAGCCGTTT+TGG 0.353112 tig0020363:-20761 None:intergenic
AGACTTGCATTGGAAGAAAA+AGG 0.354375 tig0020363:+20642 Msa1380470:CDS
GTTATGTGTGACACTAGTTT+TGG 0.361960 tig0020363:+22219 Msa1380470:three_prime_UTR
TGGAACAATGCCTTCTTGTA+AGG 0.374184 tig0020363:+22186 Msa1380470:three_prime_UTR
TTCCATACCAATCCTGTAAC+TGG 0.385351 tig0020363:+20681 Msa1380470:CDS
GAGAAGGAGCGAGGAGCATT+TGG 0.386161 tig0020363:+21476 Msa1380470:CDS
GTTTGTCTCCAGAATTTCAT+TGG 0.390685 tig0020363:+22039 Msa1380470:three_prime_UTR
CACCACACGCCTTAAGAACT+TGG 0.391896 tig0020363:-21352 None:intergenic
TTTATTTCTCCCTTACTGCT+TGG 0.399542 tig0020363:+21939 Msa1380470:three_prime_UTR
ACCAAGTACTGAAACAGTTC+AGG 0.403387 tig0020363:+22062 Msa1380470:three_prime_UTR
CTCTCATGGTTGCCAAGAAA+TGG 0.406293 tig0020363:+21809 Msa1380470:three_prime_UTR
ATCCAATACTCAGCAATGTT+TGG 0.413409 tig0020363:-21645 None:intergenic
ACGTAAACTCGGTGTTTCTC+TGG 0.433917 tig0020363:-21327 None:intergenic
GGCCAAACATTGCTGAGTAT+TGG 0.435036 tig0020363:+21643 Msa1380470:CDS
CCACCTAGGAAGTTGCTGAT+AGG 0.448934 tig0020363:-20564 None:intergenic
AGAATTGCTGTGGTCTCCGC+AGG 0.450946 tig0020363:+21210 Msa1380470:CDS
CTGAGGATATCAGTAGCAAT+AGG 0.470164 tig0020363:+21235 Msa1380470:CDS
AGAAAAGAAGAAAAGAAGAA+AGG 0.482172 tig0020363:-19642 None:intergenic
ATTCTTGCCAGTTACAGGAT+TGG 0.485946 tig0020363:-20688 None:intergenic
ACTAACATCACCAAGCAGTA+AGG 0.489289 tig0020363:-21949 None:intergenic
TTCTGGCTGTCCAAATCAAA+CGG 0.493106 tig0020363:-20615 None:intergenic
TTTGCTTAAGCGGTACTAGT+AGG 0.493567 tig0020363:+21776 Msa1380470:exon
ACAAAACATTGTTCAAAACT+TGG 0.494072 tig0020363:+21733 Msa1380470:CDS
TGCCAGTTACAGGATTGGTA+TGG 0.497143 tig0020363:-20683 None:intergenic
GCAATCACCTTTCTATAACT+AGG 0.510277 tig0020363:-21684 None:intergenic
ATATTAGAACTTTGCTTAAG+CGG 0.513812 tig0020363:+21766 Msa1380470:CDS
GGTTAGATTGCTTGATGAAG+CGG 0.515391 tig0020363:+21497 Msa1380470:CDS
ATACCTATCAGCAACTTCCT+AGG 0.523035 tig0020363:+20561 Msa1380470:five_prime_UTR
GGTATGCACCTGCTAATTCA+GGG 0.526259 tig0020363:-21395 None:intergenic
TCGGTGTTTCTCTGGTATAT+GGG 0.528301 tig0020363:-21319 None:intergenic
ATGAGAAGGGAGGAAAATCA+AGG 0.529179 tig0020363:+22002 Msa1380470:three_prime_UTR
GGATGAAGAGGATACAAGAG+TGG 0.530840 tig0020363:+21274 Msa1380470:CDS
AGTGAAACACCTTATTTGGC+AGG 0.533423 tig0020363:+21531 Msa1380470:CDS
TGTGGTGATCGCTCGTATGT+CGG 0.536691 tig0020363:+21368 Msa1380470:CDS
TCGGAGTCCCCTGAATTAGC+AGG 0.546117 tig0020363:+21387 Msa1380470:CDS
TGTGGTCTCCGCAGGGTCTG+AGG 0.546519 tig0020363:+21218 Msa1380470:CDS
CCAGAAGGTGAGACTTGCAT+TGG 0.548807 tig0020363:+20632 Msa1380470:CDS
GCAGAGGCCTAGTTATAGAA+AGG 0.552401 tig0020363:+21677 Msa1380470:CDS
ATAGGAGTGAAGTGATTGAA+TGG 0.553982 tig0020363:+21253 Msa1380470:CDS
AATCAAGGTATGCATGTAAG+TGG 0.555188 tig0020363:+22017 Msa1380470:three_prime_UTR
TTCCAAGTTCTTAAGGCGTG+TGG 0.557777 tig0020363:+21350 Msa1380470:CDS
GTTTCTCTGGTATATGGGAG+AGG 0.560412 tig0020363:-21314 None:intergenic
CACTTATCCTAGAATGAGAA+GGG 0.565199 tig0020363:+21989 Msa1380470:three_prime_UTR
CTAACATCACCAAGCAGTAA+GGG 0.567559 tig0020363:-21948 None:intergenic
CATTGTCAAAAGAAAAGAAG+TGG 0.576035 tig0020363:+22100 Msa1380470:three_prime_UTR
AGAATGAGTACATAACTATG+AGG 0.584947 tig0020363:+21622 Msa1380470:CDS
CGGGTGAAATAAAGTAGCCA+TGG 0.585967 tig0020363:-22291 None:intergenic
CGCTCCTTCTCAAAACATGA+GGG 0.586987 tig0020363:-21464 None:intergenic
CCAAGTACTGAAACAGTTCA+GGG 0.589412 tig0020363:+22063 Msa1380470:three_prime_UTR
GCACTTATCCTAGAATGAGA+AGG 0.590458 tig0020363:+21988 Msa1380470:three_prime_UTR
TCGCTCCTTCTCAAAACATG+AGG 0.593199 tig0020363:-21465 None:intergenic
AAGCAGCAATCCTTACAAGA+AGG 0.594753 tig0020363:-22196 None:intergenic
GGCAGGCGAAGAGTTTAGCA+TGG 0.595558 tig0020363:+21548 Msa1380470:CDS
GTTTGATTTGGACAGCCAGA+AGG 0.595603 tig0020363:+20617 Msa1380470:CDS
TAGGTACATATATACTCTCA+TGG 0.604593 tig0020363:+21795 Msa1380470:three_prime_UTR
AAAACAGGAAGGCTTCCACT+CGG 0.612067 tig0020363:-20738 None:intergenic
TTTCTCTGGTATATGGGAGA+GGG 0.623180 tig0020363:-21313 None:intergenic
GATGAAGAGGATACAAGAGT+GGG 0.626925 tig0020363:+21275 Msa1380470:CDS
AGAACTTGGAAACGTAAACT+CGG 0.634065 tig0020363:-21338 None:intergenic
GTACATAGAAAGAATTGCTG+TGG 0.635061 tig0020363:+21200 Msa1380470:CDS
AAGTGATTGAATGGATGAAG+AGG 0.651845 tig0020363:+21262 Msa1380470:CDS
TTCTTTCAGATGAATCCGAG+TGG 0.664252 tig0020363:+20723 Msa1380470:CDS
GAATTGCTGTGGTCTCCGCA+GGG 0.673553 tig0020363:+21211 Msa1380470:CDS
TTAGATCAAGAACGTAACCA+TGG 0.677073 tig0020363:+22274 Msa1380470:three_prime_UTR
GTATGCACCTGCTAATTCAG+GGG 0.678849 tig0020363:-21394 None:intergenic
TTATCCTAGAATGAGAAGGG+AGG 0.687206 tig0020363:+21992 Msa1380470:three_prime_UTR
CCTATCAGCAACTTCCTAGG+TGG 0.692964 tig0020363:+20564 Msa1380470:five_prime_UTR
ACTGATATCCTCAGACCCTG+CGG 0.699635 tig0020363:-21226 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGATATTTCAATATG+TGG + tig0020363:20227-20246 Msa1380470:intron 15.0%
!! TTTATCTTTAAACTACTATA+TGG - tig0020363:20920-20939 None:intergenic 15.0%
!! TCAACAAAAATAAAAAGATA+GGG - tig0020363:21900-21919 None:intergenic 15.0%
!! TTCAACAAAAATAAAAAGAT+AGG - tig0020363:21901-21920 None:intergenic 15.0%
!!! TTTATTTTTGTTGAAATAGA+AGG + tig0020363:21906-21925 Msa1380470:three_prime_UTR 15.0%
!!! TTATTTTTGTTGAAATAGAA+GGG + tig0020363:21907-21926 Msa1380470:three_prime_UTR 15.0%
!! AAAAATCAATAAATTCAGTG+AGG - tig0020363:19844-19863 None:intergenic 20.0%
!!! TATTTTAACAAACCCTTTAT+CGG + tig0020363:19886-19905 Msa1380470:intron 20.0%
!!! AGATAATTTTATCCGATAAA+GGG - tig0020363:19901-19920 None:intergenic 20.0%
!!! AAGATAATTTTATCCGATAA+AGG - tig0020363:19902-19921 None:intergenic 20.0%
!! TTCAATTTACAAAAACAAGA+CGG - tig0020363:19962-19981 None:intergenic 20.0%
!!! ATTGAAATTACTTTTTCGAT+TGG + tig0020363:19976-19995 Msa1380470:intron 20.0%
!! TAATCAAAGATGTTGAATAT+TGG + tig0020363:20009-20028 Msa1380470:intron 20.0%
!! GAAGATTTATACATCATTAT+AGG + tig0020363:20890-20909 Msa1380470:intron 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG + tig0020363:20990-21009 Msa1380470:intron 20.0%
!!! TATTTTTGTTGAAATAGAAG+GGG + tig0020363:21908-21927 Msa1380470:three_prime_UTR 20.0%
!!! TGTGTATTATTGATTGATAT+TGG + tig0020363:22166-22185 Msa1380470:three_prime_UTR 20.0%
!! AGAAAAGAAGAAAGGTTTTA+GGG - tig0020363:19637-19656 None:intergenic 25.0%
!! AAGAAAAGAAGAAAGGTTTT+AGG - tig0020363:19638-19657 None:intergenic 25.0%
! AGAAAAGAAGAAAAGAAGAA+AGG - tig0020363:19645-19664 None:intergenic 25.0%
! ACTTCAGTACTCTTTATATT+AGG + tig0020363:20080-20099 Msa1380470:intron 25.0%
! AGCACTAAAATGAAATGAAA+GGG - tig0020363:20143-20162 None:intergenic 25.0%
! TAGCACTAAAATGAAATGAA+AGG - tig0020363:20144-20163 None:intergenic 25.0%
! TGATATCACTTCTTACTTAT+AGG + tig0020363:20296-20315 Msa1380470:intron 25.0%
! AATAATGAAGAAGGTTTGAA+GGG - tig0020363:20820-20839 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG + tig0020363:21046-21065 Msa1380470:intron 25.0%
! TTCAACATTTCATTCTTAGT+GGG - tig0020363:21115-21134 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG - tig0020363:21591-21610 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG + tig0020363:21593-21612 Msa1380470:CDS 25.0%
! ACAAAACATTGTTCAAAACT+TGG + tig0020363:21733-21752 Msa1380470:CDS 25.0%
! ATATTAGAACTTTGCTTAAG+CGG + tig0020363:21766-21785 Msa1380470:CDS 25.0%
! TTACTTATTCAACCATTTCT+TGG - tig0020363:21824-21843 None:intergenic 25.0%
! AAAAATAAAAAGATAGGGCA+CGG - tig0020363:21895-21914 None:intergenic 25.0%
!! ATTTTTGTTGAAATAGAAGG+GGG + tig0020363:21909-21928 Msa1380470:three_prime_UTR 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG - tig0020363:22099-22118 None:intergenic 25.0%
! TCTTTTCTTCTTTCTTCTTC+TGG + tig0020363:19657-19676 Msa1380470:five_prime_UTR 30.0%
! CTTTTCTTCTTTCTTCTTCT+GGG + tig0020363:19658-19677 Msa1380470:five_prime_UTR 30.0%
! TGCTTTCACTTGTGTTTTAT+CGG + tig0020363:19770-19789 Msa1380470:intron 30.0%
GCATGTCAAATTTCTGTTAT+TGG + tig0020363:20186-20205 Msa1380470:intron 30.0%
TTCACATTATTCTGTCGTAA+AGG - tig0020363:20399-20418 None:intergenic 30.0%
!! GTTGTTGTTTATTGACAACT+TGG + tig0020363:20453-20472 Msa1380470:intron 30.0%
CAAAAATCACTCAGAACATT+GGG - tig0020363:20502-20521 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG + tig0020363:20605-20624 Msa1380470:CDS 30.0%
GAATAATGAAGAAGGTTTGA+AGG - tig0020363:20821-20840 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG - tig0020363:20829-20848 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG - tig0020363:20980-20999 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG - tig0020363:21037-21056 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG - tig0020363:21075-21094 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG - tig0020363:21116-21135 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG + tig0020363:21343-21362 Msa1380470:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG - tig0020363:21439-21458 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG + tig0020363:21622-21641 Msa1380470:CDS 30.0%
! GTGATTGCTAAACATTTTGA+TGG + tig0020363:21699-21718 Msa1380470:CDS 30.0%
TAGGTACATATATACTCTCA+TGG + tig0020363:21795-21814 Msa1380470:three_prime_UTR 30.0%
CATTGTCAAAAGAAAAGAAG+TGG + tig0020363:22100-22119 Msa1380470:three_prime_UTR 30.0%
!!! AAGGTTTTAGGGATTGAAAG+TGG - tig0020363:19626-19645 None:intergenic 35.0%
! TCACTTGTGTTTTATCGGAT+TGG + tig0020363:19775-19794 Msa1380470:intron 35.0%
ACAATGTAAGAAAGATTGGC+AGG - tig0020363:20352-20371 None:intergenic 35.0%
CGAGACAATGTAAGAAAGAT+TGG - tig0020363:20356-20375 None:intergenic 35.0%
CCAAAAATCACTCAGAACAT+TGG - tig0020363:20503-20522 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG + tig0020363:20500-20519 Msa1380470:five_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG + tig0020363:20506-20525 Msa1380470:five_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG - tig0020363:20581-20600 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG + tig0020363:20642-20661 Msa1380470:CDS 35.0%
CGGATTCATCTGAAAGAATA+AGG - tig0020363:20721-20740 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG + tig0020363:20830-20849 Msa1380470:intron 35.0%
ACAAATGTCTTACAGTGCTA+TGG + tig0020363:21168-21187 Msa1380470:intron 35.0%
! TACAGTGCTATGGATTTTTC+AGG + tig0020363:21178-21197 Msa1380470:intron 35.0%
GTACATAGAAAGAATTGCTG+TGG + tig0020363:21200-21219 Msa1380470:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG + tig0020363:21253-21272 Msa1380470:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG + tig0020363:21262-21281 Msa1380470:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG - tig0020363:21303-21322 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG - tig0020363:21341-21360 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG + tig0020363:21527-21546 Msa1380470:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG - tig0020363:21648-21667 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG + tig0020363:21650-21669 Msa1380470:CDS 35.0%
GCAATCACCTTTCTATAACT+AGG - tig0020363:21687-21706 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG + tig0020363:21703-21722 Msa1380470:CDS 35.0%
! TTTATTTCTCCCTTACTGCT+TGG + tig0020363:21939-21958 Msa1380470:three_prime_UTR 35.0%
CACTTATCCTAGAATGAGAA+GGG + tig0020363:21989-22008 Msa1380470:three_prime_UTR 35.0%
AATCAAGGTATGCATGTAAG+TGG + tig0020363:22017-22036 Msa1380470:three_prime_UTR 35.0%
GTTTGTCTCCAGAATTTCAT+TGG + tig0020363:22039-22058 Msa1380470:three_prime_UTR 35.0%
TACTTGGTCCAATGAAATTC+TGG - tig0020363:22050-22069 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG - tig0020363:22092-22111 None:intergenic 35.0%
!! GTTATGTGTGACACTAGTTT+TGG + tig0020363:22219-22238 Msa1380470:three_prime_UTR 35.0%
TTAGATCAAGAACGTAACCA+TGG + tig0020363:22274-22293 Msa1380470:three_prime_UTR 35.0%
AAGTGGAGAGAGTGAGAAAA+AGG - tig0020363:19609-19628 None:intergenic 40.0%
! TATAGGCTTCTGTCACGTTT+TGG + tig0020363:20313-20332 Msa1380470:intron 40.0%
ATACCTATCAGCAACTTCCT+AGG + tig0020363:20561-20580 Msa1380470:five_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG - tig0020363:20618-20637 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG + tig0020363:20681-20700 Msa1380470:CDS 40.0%
ATTCTTGCCAGTTACAGGAT+TGG - tig0020363:20691-20710 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG + tig0020363:20692-20711 Msa1380470:CDS 40.0%
TCCAAATTCTTGCCAGTTAC+AGG - tig0020363:20696-20715 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG + tig0020363:20723-20742 Msa1380470:CDS 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG + tig0020363:20747-20766 Msa1380470:CDS 40.0%
! AAATGATGTGAGAGCCGTTT+TGG - tig0020363:20764-20783 None:intergenic 40.0%
TATACACAATAGGCTGCTTC+AGG - tig0020363:21027-21046 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG + tig0020363:21235-21254 Msa1380470:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG + tig0020363:21275-21294 Msa1380470:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG - tig0020363:21316-21335 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG - tig0020363:21322-21341 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG - tig0020363:21419-21438 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG + tig0020363:21460-21479 Msa1380470:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG + tig0020363:21497-21516 Msa1380470:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG + tig0020363:21531-21550 Msa1380470:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG + tig0020363:21661-21680 Msa1380470:CDS 40.0%
TTTGCTTAAGCGGTACTAGT+AGG + tig0020363:21776-21795 Msa1380470:exon 40.0%
CTAACATCACCAAGCAGTAA+GGG - tig0020363:21951-21970 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG - tig0020363:21952-21971 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG + tig0020363:21988-22007 Msa1380470:three_prime_UTR 40.0%
TTATCCTAGAATGAGAAGGG+AGG + tig0020363:21992-22011 Msa1380470:three_prime_UTR 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG - tig0020363:21999-22018 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG + tig0020363:22002-22021 Msa1380470:three_prime_UTR 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG + tig0020363:22062-22081 Msa1380470:three_prime_UTR 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG + tig0020363:22063-22082 Msa1380470:three_prime_UTR 40.0%
TGGAACAATGCCTTCTTGTA+AGG + tig0020363:22186-22205 Msa1380470:three_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG - tig0020363:22199-22218 None:intergenic 40.0%
!! GTTTGATTTGGACAGCCAGA+AGG + tig0020363:20617-20636 Msa1380470:CDS 45.0%
! TGCCAGTTACAGGATTGGTA+TGG - tig0020363:20686-20705 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG - tig0020363:20741-20760 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG - tig0020363:20752-20771 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG - tig0020363:20756-20775 None:intergenic 45.0%
! ATAGGCTGCTTCAGGTTCAT+GGG - tig0020363:21019-21038 None:intergenic 45.0%
! AATAGGCTGCTTCAGGTTCA+TGG - tig0020363:21020-21039 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG + tig0020363:21274-21293 Msa1380470:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG - tig0020363:21317-21336 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG - tig0020363:21323-21342 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG - tig0020363:21330-21349 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG + tig0020363:21350-21369 Msa1380470:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG - tig0020363:21397-21416 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG - tig0020363:21398-21417 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG - tig0020363:21399-21418 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG - tig0020363:21467-21486 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG - tig0020363:21468-21487 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG + tig0020363:21467-21486 Msa1380470:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG - tig0020363:21543-21562 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG + tig0020363:21643-21662 Msa1380470:CDS 45.0%
GCAGAGGCCTAGTTATAGAA+AGG + tig0020363:21677-21696 Msa1380470:CDS 45.0%
CTCTCATGGTTGCCAAGAAA+TGG + tig0020363:21809-21828 Msa1380470:three_prime_UTR 45.0%
CCCTGAACTGTTTCAGTACT+TGG - tig0020363:22066-22085 None:intergenic 45.0%
CGGGTGAAATAAAGTAGCCA+TGG - tig0020363:22294-22313 None:intergenic 45.0%
!! AAAATTGATATATTAATAAA+TGG - tig0020363:20270-20289 None:intergenic 5.0%
CCACCTAGGAAGTTGCTGAT+AGG - tig0020363:20567-20586 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG + tig0020363:20564-20583 Msa1380470:five_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG - tig0020363:20635-20654 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG + tig0020363:20632-20651 Msa1380470:CDS 50.0%
ACTGATATCCTCAGACCCTG+CGG - tig0020363:21229-21248 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG - tig0020363:21355-21374 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG + tig0020363:21368-21387 Msa1380470:CDS 50.0%
AGAATTGCTGTGGTCTCCGC+AGG + tig0020363:21210-21229 Msa1380470:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG + tig0020363:21211-21230 Msa1380470:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG + tig0020363:21387-21406 Msa1380470:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG + tig0020363:21476-21495 Msa1380470:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG + tig0020363:21548-21567 Msa1380470:CDS 55.0%
TGTGGTCTCCGCAGGGTCTG+AGG + tig0020363:21218-21237 Msa1380470:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0020363 gene 19563 22316 19563 ID=Msa1380470;Name=Msa1380470
tig0020363 mRNA 19563 22316 19563 ID=Msa1380470-mRNA-1;Parent=Msa1380470;Name=Msa1380470-mRNA-1;_AED=0.17;_eAED=0.17;_QI=217|1|1|1|0.5|0.33|3|522|267
tig0020363 exon 19563 19679 19563 ID=Msa1380470-mRNA-1:exon:7139;Parent=Msa1380470-mRNA-1
tig0020363 exon 20497 20805 20497 ID=Msa1380470-mRNA-1:exon:7140;Parent=Msa1380470-mRNA-1
tig0020363 exon 21200 22316 21200 ID=Msa1380470-mRNA-1:exon:7141;Parent=Msa1380470-mRNA-1
tig0020363 five_prime_UTR 19563 19679 19563 ID=Msa1380470-mRNA-1:five_prime_utr;Parent=Msa1380470-mRNA-1
tig0020363 five_prime_UTR 20497 20596 20497 ID=Msa1380470-mRNA-1:five_prime_utr;Parent=Msa1380470-mRNA-1
tig0020363 CDS 20597 20805 20597 ID=Msa1380470-mRNA-1:cds;Parent=Msa1380470-mRNA-1
tig0020363 CDS 21200 21794 21200 ID=Msa1380470-mRNA-1:cds;Parent=Msa1380470-mRNA-1
tig0020363 three_prime_UTR 21795 22316 21795 ID=Msa1380470-mRNA-1:three_prime_utr;Parent=Msa1380470-mRNA-1
Gene Sequence

>Msa1380470

ATGCAGCTATATCATCATCCGTTTGATTTGGACAGCCAGAAGGTGAGACTTGCATTGGAAGAAAAAGGCATTGATTATACATCTTTCCATACCAATCCTGTAACTGGCAAGAATTTGGATTCCTTATTCTTTCAGATGAATCCGAGTGGAAGCCTTCCTGTTTTCCAAAACGGCTCTCACATCATTTACAAGACTATTGATATAATTCAGTACATAGAAAGAATTGCTGTGGTCTCCGCAGGGTCTGAGGATATCAGTAGCAATAGGAGTGAAGTGATTGAATGGATGAAGAGGATACAAGAGTGGGATCCTAAATATTTTTCCCTCTCCCATATACCAGAGAAACACCGAGTTTACGTTTCCAAGTTCTTAAGGCGTGTGGTGATCGCTCGTATGTCGGAGTCCCCTGAATTAGCAGGTGCATACCATAGAAAGTTGAGAGAAGCCTATCAAACTGAAGAAAAGTTGAAAGACCCTCATGTTTTGAGAAGGAGCGAGGAGCATTTGGTTAGATTGCTTGATGAAGCGGAAAGACAACTGAGTGAAACACCTTATTTGGCAGGCGAAGAGTTTAGCATGGCTGATGTGATGCTCATTCCGATTTTAGCTCGTTTAAAACTTTTGGATTTAGAGAATGAGTACATAACTATGAGGCCAAACATTGCTGAGTATTGGATTTTGGTTCAGCAGAGGCCTAGTTATAGAAAGGTGATTGCTAAACATTTTGATGGCTGGAGAAAGCACAAAACATTGTTCAAAACTTGGTGCTTTGTTCATATTAGAACTTTGCTTAAGCGGTACTAG
Protein sequence

>Msa1380470

MQLYHHPFDLDSQKVRLALEEKGIDYTSFHTNPVTGKNLDSLFFQMNPSGSLPVFQNGSHIIYKTIDIIQYIERIAVVSAGSEDISSNRSEVIEWMKRIQEWDPKYFSLSHIPEKHRVYVSKFLRRVVIARMSESPELAGAYHRKLREAYQTEEKLKDPHVLRRSEEHLVRLLDEAERQLSETPYLAGEEFSMADVMLIPILARLKLLDLENEYITMRPNIAEYWILVQQRPSYRKVIAKHFDGWRKHKTLFKTWCFVHIRTLLKRY*