AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0235210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235210 MtrunA17_Chr2g0283691 96.825 441 13 1 1 441 1 440 0.0 861
Msa0235210 MtrunA17_Chr3g0137061 33.943 383 223 6 65 419 90 470 6.32e-46 166
Msa0235210 MtrunA17_Chr1g0149941 34.870 347 191 8 105 419 192 535 7.05e-40 150
Msa0235210 MtrunA17_Chr8g0377151 27.302 315 218 4 61 367 402 713 3.81e-20 93.6
Msa0235210 MtrunA17_Chr3g0122721 29.301 372 237 13 55 414 398 755 5.27e-20 92.8
Msa0235210 MtrunA17_Chr1g0183781 28.269 283 198 5 87 368 440 718 4.08e-18 87.0
Msa0235210 MtrunA17_Chr1g0152561 25.685 292 211 5 81 370 55 342 1.37e-13 71.6
Msa0235210 MtrunA17_Chr7g0261371 25.524 286 208 4 87 371 514 795 1.58e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235210 AT2G27430.1 56.948 439 169 7 10 441 13 438 1.70e-155 447
Msa0235210 AT2G25130.1 35.583 326 189 7 108 419 132 450 1.03e-43 159
Msa0235210 AT4G31890.3 36.705 346 189 11 97 419 155 493 2.41e-43 159
Msa0235210 AT4G31890.2 36.705 346 189 11 97 419 155 493 2.41e-43 159
Msa0235210 AT4G31890.1 36.705 346 189 11 97 419 155 493 2.41e-43 159
Msa0235210 AT1G67530.2 29.293 297 180 8 100 379 439 722 4.94e-18 87.4
Msa0235210 AT1G67530.1 29.293 297 180 8 100 379 439 722 4.94e-18 87.4
Msa0235210 AT1G67530.4 29.293 297 180 8 100 379 439 722 4.94e-18 87.4
Msa0235210 AT2G23140.3 25.364 343 248 7 30 371 300 635 1.44e-16 82.4
Msa0235210 AT2G23140.5 25.364 343 248 7 30 371 300 635 1.44e-16 82.4
Msa0235210 AT2G23140.2 25.364 343 248 7 30 371 489 824 2.23e-16 82.4
Msa0235210 AT2G23140.4 25.364 343 248 7 30 371 489 824 2.23e-16 82.4
Msa0235210 AT2G23140.1 25.364 343 248 7 30 371 492 827 2.29e-16 82.0
Msa0235210 AT1G27910.1 27.760 317 214 6 73 377 404 717 3.44e-14 75.5
Msa0235210 AT3G54790.2 28.873 284 196 6 89 370 440 719 3.93e-14 75.1
Msa0235210 AT3G54790.3 28.873 284 196 6 89 370 476 755 5.56e-14 74.7
Msa0235210 AT3G54790.1 28.873 284 196 6 89 370 476 755 5.56e-14 74.7
Msa0235210 AT1G24330.1 25.773 291 189 5 112 387 451 729 3.42e-13 72.4
Msa0235210 AT1G24330.2 25.773 291 189 5 112 387 451 729 3.42e-13 72.4

Find 109 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTATTGCAATGGTTTA+AGG 0.113364 2_2:-8049921 Msa0235210:CDS
GTCAATTGTCTTAGGTAATT+TGG 0.115729 2_2:+8050474 None:intergenic
TTAATATAAACGGTTGAATT+TGG 0.144794 2_2:-8049475 Msa0235210:three_prime_UTR
CTTCATCGTCTTCTTCTATT+TGG 0.148113 2_2:-8052534 Msa0235210:CDS
ACCGACAACTCTTGACATTT+TGG 0.155414 2_2:+8050070 None:intergenic
TTTGTATCTCTTTGATTTAA+AGG 0.178789 2_2:+8049833 None:intergenic
ACAAAGCTTGCATAAGAATT+TGG 0.181591 2_2:-8049777 Msa0235210:CDS
TAACTTGTTGGTCACGTTAA+TGG 0.225258 2_2:-8050164 Msa0235210:CDS
CGGAGGATTCACAGCGCTTT+CGG 0.245617 2_2:+8052446 None:intergenic
TGTAGGTGCTACAGCAATTC+AGG 0.257515 2_2:-8049434 Msa0235210:three_prime_UTR
GGAGGATTCACAGCGCTTTC+GGG 0.279689 2_2:+8052447 None:intergenic
TTCATCACAAAGAGATAAAA+TGG 0.283330 2_2:-8050017 Msa0235210:CDS
GCAAGCCCTTTAGTTTCTTA+TGG 0.289989 2_2:-8050256 Msa0235210:CDS
ACTTGGGGTGGTGCCGGTTT+TGG 0.292683 2_2:-8052170 Msa0235210:CDS
TTCTGAAATTTCTTTGACTA+AGG 0.295883 2_2:+8050207 None:intergenic
CTTTAATTCAACAACTTTGT+AGG 0.304965 2_2:-8049451 Msa0235210:three_prime_UTR
TTCATCGTCTTCTTCTATTT+GGG 0.313407 2_2:-8052533 Msa0235210:CDS
AAGGAAAGTTCTAAGAGAAT+TGG 0.315481 2_2:+8050226 None:intergenic
GGCTCCTAAACATGATCTTT+TGG 0.319278 2_2:+8050309 None:intergenic
GGAGGAGGAGAGGGAGGAAG+TGG 0.329855 2_2:-8052326 Msa0235210:CDS
ACGGTGGACCGTTGCCATTT+TGG 0.339704 2_2:-8049595 Msa0235210:three_prime_UTR
ATTCCAAATCTTGGTGTTTG+TGG 0.341246 2_2:+8049863 None:intergenic
CAAAGTTATAGTGTCGATTT+CGG 0.346294 2_2:-8049547 Msa0235210:three_prime_UTR
AATAACCAATGACTTGAGTT+TGG 0.347977 2_2:+8049715 None:intergenic
AACTTGTTGGTCACGTTAAT+GGG 0.351557 2_2:-8050163 Msa0235210:CDS
TCTACAAGGTCAATTGTCTT+AGG 0.352811 2_2:+8050466 None:intergenic
GGTCCACAAACACCAAGATT+TGG 0.367878 2_2:-8049866 Msa0235210:CDS
GATACCAAAAGATCATGTTT+AGG 0.369581 2_2:-8050313 Msa0235210:CDS
ACAGCGCTTTCGGGTTGCTT+TGG 0.370779 2_2:+8052456 None:intergenic
TAACTCTGCAAACTCACTTA+TGG 0.378473 2_2:+8050435 None:intergenic
TTAAGAATGTCTTTAAGAAA+TGG 0.384957 2_2:+8050352 None:intergenic
CGAAATCGACACTATAACTT+TGG 0.389649 2_2:+8049548 None:intergenic
GTAAAGATGGGACCACATTC+TGG 0.405463 2_2:-8049887 Msa0235210:CDS
AGAAAATCTAATGAAGGAAA+AGG 0.408091 2_2:+8050376 None:intergenic
AAATGCTTCACTTTGGAAGC+TGG 0.408100 2_2:-8052270 Msa0235210:CDS
CTAATGAAGGAAAAGGGTAT+TGG 0.408170 2_2:+8050383 None:intergenic
TAATGAAGGAAAAGGGTATT+GGG 0.409389 2_2:+8050384 None:intergenic
ACTCAAAGGGCAAATGCAAA+TGG 0.411229 2_2:-8049746 Msa0235210:CDS
CATGAATATCTTAATATAAA+CGG 0.413463 2_2:-8049485 Msa0235210:three_prime_UTR
TGCTACTTTAGGTAACTTGT+TGG 0.425049 2_2:-8050176 Msa0235210:CDS
GAAGCATAAGGAGGAGGAGA+GGG 0.427827 2_2:-8052335 Msa0235210:CDS
CTCAAAGGGCAAATGCAAAT+GGG 0.429397 2_2:-8049745 Msa0235210:CDS
CCATGAGTTCGACATGATTA+CGG 0.431320 2_2:-8049614 Msa0235210:three_prime_UTR
GGTATCTATGGTGGCGTCGC+AGG 0.433254 2_2:-8052149 Msa0235210:CDS
AGGAAAGTTCTAAGAGAATT+GGG 0.434479 2_2:+8050227 None:intergenic
GCGGTTAGTCACCGGAGAGC+AGG 0.438079 2_2:-8052127 Msa0235210:CDS
TATGGAGAAGCATAAGGAGG+AGG 0.454646 2_2:-8052341 Msa0235210:CDS
TTTGCAGCCACACAGAGATT+CGG 0.456423 2_2:+8049515 None:intergenic
ACCAAAATGTCAAGAGTTGT+CGG 0.458853 2_2:-8050071 Msa0235210:CDS
GGTGACCCCATTCCAAATCT+TGG 0.459651 2_2:+8049854 None:intergenic
AAAACAACTTCAAGAAGTAT+AGG 0.466913 2_2:+8049977 None:intergenic
AACAGAACTTGGGGTGGTGC+CGG 0.471789 2_2:-8052176 Msa0235210:CDS
AATGCTTCACTTTGGAAGCT+GGG 0.475011 2_2:-8052269 Msa0235210:CDS
GTCCTCCAAACTCAAGTCAT+TGG 0.475448 2_2:-8049720 Msa0235210:CDS
GCATAAGGAGGAGGAGAGGG+AGG 0.477154 2_2:-8052332 Msa0235210:CDS
GAAAATCTAATGAAGGAAAA+GGG 0.479557 2_2:+8050377 None:intergenic
GATTGAAACAGTGCAAGTTA+TGG 0.479849 2_2:-8052359 Msa0235210:CDS
AATCAAAGAGATACAAATGA+AGG 0.481857 2_2:-8049827 Msa0235210:CDS
GAAAGTTGATAACAGAACTT+GGG 0.489408 2_2:-8052186 Msa0235210:CDS
TTTGGAAATAATAACTCAAA+GGG 0.489930 2_2:-8049759 Msa0235210:CDS
CATTGTTGAAAGGTTGTACA+AGG 0.492312 2_2:+8050288 None:intergenic
GGATGAAAGACAAGTAAAGA+TGG 0.494341 2_2:-8049900 Msa0235210:CDS
AGAAAGTTGATAACAGAACT+TGG 0.494673 2_2:-8052187 Msa0235210:CDS
CAAGACCATAAGAAACTAAA+GGG 0.497099 2_2:+8050251 None:intergenic
CAGGAACAAGGCCATGATAG+TGG 0.500895 2_2:-8050512 Msa0235210:CDS
ACAGTGAAAATGCTTCACTT+TGG 0.503088 2_2:-8052277 Msa0235210:CDS
TCTTCAATAAGACAAGCTCT+TGG 0.504288 2_2:+8049682 None:intergenic
AACCAATGACTTGAGTTTGG+AGG 0.512382 2_2:+8049718 None:intergenic
CAAACACCAAGATTTGGAAT+GGG 0.513089 2_2:-8049860 Msa0235210:CDS
TATTCCTGCCTCCACTATCA+TGG 0.515130 2_2:+8050501 None:intergenic
GCAAGTTATGGAGAAGCATA+AGG 0.517239 2_2:-8052347 Msa0235210:CDS
ACGCCGTCAACCCTGCTCTC+CGG 0.518209 2_2:+8052116 None:intergenic
GAGATAAAATGGCACAAGCT+AGG 0.518471 2_2:-8050006 Msa0235210:CDS
AATTCTTTACTCGTATCAAA+CGG 0.519898 2_2:-8052489 Msa0235210:CDS
TCACCGGAGAGCAGGGTTGA+CGG 0.520564 2_2:-8052119 Msa0235210:CDS
AATGGAAGAAAATCTAATGA+AGG 0.520831 2_2:+8050370 None:intergenic
TGGAAGCTGGGAAGAGAAAG+AGG 0.523058 2_2:-8052257 Msa0235210:CDS
CGATGAAGGATTTGACATGT+TGG 0.532298 2_2:+8052549 None:intergenic
CAAATAGAAGAAGACGATGA+AGG 0.536497 2_2:+8052535 None:intergenic
ACAAGACCATAAGAAACTAA+AGG 0.539120 2_2:+8050250 None:intergenic
ACAAACACCAAGATTTGGAA+TGG 0.539160 2_2:-8049861 Msa0235210:CDS
GTGTTCTTTCTTCAGGAACA+AGG 0.540830 2_2:-8050524 Msa0235210:intron
GAGCAATGAAACTATTGCAA+TGG 0.549239 2_2:-8049928 Msa0235210:CDS
GCCACCATAGATACCAAAAC+CGG 0.549653 2_2:+8052157 None:intergenic
CGGTTAGTCACCGGAGAGCA+GGG 0.553101 2_2:-8052126 Msa0235210:CDS
AGAAGCATAAGGAGGAGGAG+AGG 0.553180 2_2:-8052336 Msa0235210:CDS
GCGTTGATTTACCTAGCTGA+TGG 0.553971 2_2:-8052097 Msa0235210:CDS
CCGTAATCATGTCGAACTCA+TGG 0.560647 2_2:+8049614 None:intergenic
GATGAAAGACAAGTAAAGAT+GGG 0.560923 2_2:-8049899 Msa0235210:CDS
TTCTTTGTTTAAATAATCGG+AGG 0.562901 2_2:+8052429 None:intergenic
CGTCGCAGGCGGTTAGTCAC+CGG 0.566256 2_2:-8052135 Msa0235210:CDS
ATTTGGAAATAATAACTCAA+AGG 0.567717 2_2:-8049760 Msa0235210:CDS
ATCTATGGTGGCGTCGCAGG+CGG 0.569326 2_2:-8052146 Msa0235210:CDS
AGCAATAGAGAACAACTCAA+TGG 0.577734 2_2:-8050134 Msa0235210:CDS
GGTGTTTGTGGACCAGAATG+TGG 0.586193 2_2:+8049875 None:intergenic
AAGGCCATGATAGTGGAGGC+AGG 0.586256 2_2:-8050505 Msa0235210:CDS
ATCAAAGAGATACAAATGAA+GGG 0.587935 2_2:-8049826 Msa0235210:CDS
ATGGTCAATTCAAACATACA+TGG 0.591645 2_2:+8049633 None:intergenic
TCAAAGGGCAAATGCAAATG+GGG 0.635611 2_2:-8049744 Msa0235210:CDS
TTATGGTTGACTCATCTACA+AGG 0.635954 2_2:+8050452 None:intergenic
ACGTGTAAGTTCCATCAGCT+AGG 0.637555 2_2:+8052086 None:intergenic
GAGCAAGATTGAACAGAACC+AGG 0.641982 2_2:-8052389 Msa0235210:CDS
AAAGTTGATAACAGAACTTG+GGG 0.654440 2_2:-8052185 Msa0235210:CDS
AAACACCAAGATTTGGAATG+GGG 0.656392 2_2:-8049859 Msa0235210:CDS
GAACAAGGCCATGATAGTGG+AGG 0.670199 2_2:-8050509 Msa0235210:CDS
ATCTCAGCCGAATCTCTGTG+TGG 0.678767 2_2:-8049522 Msa0235210:three_prime_UTR
AGTTATGGAGAAGCATAAGG+AGG 0.680310 2_2:-8052344 Msa0235210:CDS
TGAGTTCGACATGATTACGG+TGG 0.717362 2_2:-8049611 Msa0235210:three_prime_UTR
GTTGATAACAGAACTTGGGG+TGG 0.719305 2_2:-8052182 Msa0235210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCTTTGTTTAAATAAT+CGG + chr2_2:8049564-8049583 None:intergenic 10.0%
!!! ATATTTCTTTTATTCTTTAT+AGG - chr2_2:8050430-8050449 Msa0235210:CDS 10.0%
!!! CTCAAAAAAAAATATTATTT+TGG + chr2_2:8050487-8050506 None:intergenic 10.0%
!!! CAAAATAATATTTTTTTTTG+AGG - chr2_2:8050485-8050504 Msa0235210:CDS 10.0%
!! ATTATATTTATACAAGAAAT+TGG - chr2_2:8050680-8050699 Msa0235210:intron 10.0%
!! TTATATTTATACAAGAAATT+GGG - chr2_2:8050681-8050700 Msa0235210:intron 10.0%
!! TTAATGCAATTTAATTAATT+TGG + chr2_2:8050994-8051013 None:intergenic 10.0%
!! ATGTTATTCCAAAATTAATA+CGG - chr2_2:8050608-8050627 Msa0235210:intron 15.0%
!! ACTCTTAAATTTATTATACT+CGG - chr2_2:8050639-8050658 Msa0235210:intron 15.0%
!! CTTGTATAAATATAATTAAG+TGG + chr2_2:8050676-8050695 None:intergenic 15.0%
!! TATATTTATACAAGAAATTG+GGG - chr2_2:8050682-8050701 Msa0235210:intron 15.0%
!!! GAAATTAACATTTAAAATAG+AGG - chr2_2:8050888-8050907 Msa0235210:intron 15.0%
!! ATTAATTTCTTGTATAATTG+AGG + chr2_2:8051269-8051288 None:intergenic 15.0%
!! CATGAATATCTTAATATAAA+CGG - chr2_2:8052502-8052521 Msa0235210:CDS 15.0%
!! ATTACTTCTACTAAATTTAC+AGG - chr2_2:8050063-8050082 Msa0235210:CDS 20.0%
!! TTACTTCTACTAAATTTACA+GGG - chr2_2:8050064-8050083 Msa0235210:CDS 20.0%
!!! GATATAAACCGTATTAATTT+TGG + chr2_2:8050619-8050638 None:intergenic 20.0%
!! CAAGATTTATTAATACAAAG+TGG - chr2_2:8050772-8050791 Msa0235210:intron 20.0%
!! TTATTAATACAAAGTGGTTA+TGG - chr2_2:8050778-8050797 Msa0235210:intron 20.0%
!! TATTAATACAAAGTGGTTAT+GGG - chr2_2:8050779-8050798 Msa0235210:intron 20.0%
!! GAATTTGAAGAATAATAGTT+TGG + chr2_2:8050843-8050862 None:intergenic 20.0%
!! ATATTAAATCACCAAAATGA+TGG - chr2_2:8051132-8051151 Msa0235210:intron 20.0%
!! TATTAAATCACCAAAATGAT+GGG - chr2_2:8051133-8051152 Msa0235210:intron 20.0%
!! AATTGTCATAAATATAGTGT+AGG - chr2_2:8051190-8051209 Msa0235210:intron 20.0%
!!! TTTAATTTGGTCAAAAAGTT+AGG + chr2_2:8051411-8051430 None:intergenic 20.0%
!!! TTAAGAATGTCTTTAAGAAA+TGG + chr2_2:8051638-8051657 None:intergenic 20.0%
!!! AAAATCTCAACAAAATTTTG+TGG + chr2_2:8051881-8051900 None:intergenic 20.0%
!!! TTTGTATCTCTTTGATTTAA+AGG + chr2_2:8052157-8052176 None:intergenic 20.0%
!! ATTTGGAAATAATAACTCAA+AGG - chr2_2:8052227-8052246 Msa0235210:CDS 20.0%
!! TTTGGAAATAATAACTCAAA+GGG - chr2_2:8052228-8052247 Msa0235210:CDS 20.0%
!! TTAATATAAACGGTTGAATT+TGG - chr2_2:8052512-8052531 Msa0235210:CDS 20.0%
! AATTCTTTACTCGTATCAAA+CGG - chr2_2:8049498-8049517 Msa0235210:three_prime_UTR 25.0%
!! TTCTTTGTTTAAATAATCGG+AGG + chr2_2:8049561-8049580 None:intergenic 25.0%
! TAGAGACATTTCATATATTG+TGG + chr2_2:8050004-8050023 None:intergenic 25.0%
!! GGAATATTTAGCCAATATTT+TGG - chr2_2:8051033-8051052 Msa0235210:intron 25.0%
! GAAATTTGATGCCAAAATAT+TGG + chr2_2:8051047-8051066 None:intergenic 25.0%
!!! GAAATAGGCATGTTTTAATT+TGG + chr2_2:8051424-8051443 None:intergenic 25.0%
! GTTAGATAAAGAAAGGAAAT+AGG + chr2_2:8051439-8051458 None:intergenic 25.0%
! GAAAATCTAATGAAGGAAAA+GGG + chr2_2:8051613-8051632 None:intergenic 25.0%
! AGAAAATCTAATGAAGGAAA+AGG + chr2_2:8051614-8051633 None:intergenic 25.0%
! AATGGAAGAAAATCTAATGA+AGG + chr2_2:8051620-8051639 None:intergenic 25.0%
!! CATTTTCTAACATTGTTGAA+AGG + chr2_2:8051712-8051731 None:intergenic 25.0%
!!! TTCTGAAATTTCTTTGACTA+AGG + chr2_2:8051783-8051802 None:intergenic 25.0%
!! ATTTTGTTGAGATTTTGTCA+TGG - chr2_2:8051885-8051904 Msa0235210:intron 25.0%
!! TTTTGTTGAGATTTTGTCAT+GGG - chr2_2:8051886-8051905 Msa0235210:intron 25.0%
! TTCATCACAAAGAGATAAAA+TGG - chr2_2:8051970-8051989 Msa0235210:intron 25.0%
!! AAAACAACTTCAAGAAGTAT+AGG + chr2_2:8052013-8052032 None:intergenic 25.0%
!!! ACTTCTTGAAGTTGTTTTAT+TGG - chr2_2:8052015-8052034 Msa0235210:intron 25.0%
!!! CTTCTTGAAGTTGTTTTATT+GGG - chr2_2:8052016-8052035 Msa0235210:intron 25.0%
!!! TTCTTGAAGTTGTTTTATTG+GGG - chr2_2:8052017-8052036 Msa0235210:intron 25.0%
! AATCAAAGAGATACAAATGA+AGG - chr2_2:8052160-8052179 Msa0235210:CDS 25.0%
! ATCAAAGAGATACAAATGAA+GGG - chr2_2:8052161-8052180 Msa0235210:CDS 25.0%
! GAAAAAATTGATGAAGAGTT+TGG - chr2_2:8052183-8052202 Msa0235210:CDS 25.0%
! CTTTAATTCAACAACTTTGT+AGG - chr2_2:8052536-8052555 Msa0235210:CDS 25.0%
TTCATCGTCTTCTTCTATTT+GGG - chr2_2:8049454-8049473 Msa0235210:three_prime_UTR 30.0%
!! AGAAAGTTGATAACAGAACT+TGG - chr2_2:8049800-8049819 Msa0235210:CDS 30.0%
!! GAAAGTTGATAACAGAACTT+GGG - chr2_2:8049801-8049820 Msa0235210:CDS 30.0%
!! AAAGTTGATAACAGAACTTG+GGG - chr2_2:8049802-8049821 Msa0235210:CDS 30.0%
! ATTAGTGCTGAATTTGAACA+TGG - chr2_2:8050313-8050332 Msa0235210:CDS 30.0%
! TTAGTGCTGAATTTGAACAT+GGG - chr2_2:8050314-8050333 Msa0235210:CDS 30.0%
!!! TAACCACTGATTTTGACTTT+AGG - chr2_2:8050738-8050757 Msa0235210:intron 30.0%
!!! AACCACTGATTTTGACTTTA+GGG - chr2_2:8050739-8050758 Msa0235210:intron 30.0%
GAAGAATAATAGTTTGGTTG+TGG + chr2_2:8050837-8050856 None:intergenic 30.0%
AATGACATCAAATCTGATGA+TGG - chr2_2:8051012-8051031 Msa0235210:intron 30.0%
TTTCTATGTGAAGTGTACTA+TGG + chr2_2:8051090-8051109 None:intergenic 30.0%
ACTTCACATAGAAATTGAGT+TGG - chr2_2:8051096-8051115 Msa0235210:intron 30.0%
TCATTCAGAGTAAAGTCAAT+GGG + chr2_2:8051302-8051321 None:intergenic 30.0%
ATCATTCAGAGTAAAGTCAA+TGG + chr2_2:8051303-8051322 None:intergenic 30.0%
GTCAATTGTCTTAGGTAATT+TGG + chr2_2:8051516-8051535 None:intergenic 30.0%
!! TAATGAAGGAAAAGGGTATT+GGG + chr2_2:8051606-8051625 None:intergenic 30.0%
GATACCAAAAGATCATGTTT+AGG - chr2_2:8051674-8051693 Msa0235210:intron 30.0%
CAAGACCATAAGAAACTAAA+GGG + chr2_2:8051739-8051758 None:intergenic 30.0%
ACAAGACCATAAGAAACTAA+AGG + chr2_2:8051740-8051759 None:intergenic 30.0%
!! AGGAAAGTTCTAAGAGAATT+GGG + chr2_2:8051763-8051782 None:intergenic 30.0%
!! AAGGAAAGTTCTAAGAGAAT+TGG + chr2_2:8051764-8051783 None:intergenic 30.0%
!! GAAAAAGTTCTTGCTACTTT+AGG - chr2_2:8051800-8051819 Msa0235210:intron 30.0%
GAAACTATTGCAATGGTTTA+AGG - chr2_2:8052066-8052085 Msa0235210:intron 30.0%
GATGAAAGACAAGTAAAGAT+GGG - chr2_2:8052088-8052107 Msa0235210:CDS 30.0%
ACAAAGCTTGCATAAGAATT+TGG - chr2_2:8052210-8052229 Msa0235210:CDS 30.0%
AATAACCAATGACTTGAGTT+TGG + chr2_2:8052275-8052294 None:intergenic 30.0%
ATGGTCAATTCAAACATACA+TGG + chr2_2:8052357-8052376 None:intergenic 30.0%
!! CAAAGTTATAGTGTCGATTT+CGG - chr2_2:8052440-8052459 Msa0235210:CDS 30.0%
CAAATAGAAGAAGACGATGA+AGG + chr2_2:8049455-8049474 None:intergenic 35.0%
CTTCATCGTCTTCTTCTATT+TGG - chr2_2:8049453-8049472 Msa0235210:three_prime_UTR 35.0%
! CTGTTTCAATCTTTTCAACC+TGG + chr2_2:8049619-8049638 None:intergenic 35.0%
GATTGAAACAGTGCAAGTTA+TGG - chr2_2:8049628-8049647 Msa0235210:three_prime_UTR 35.0%
ACAGTGAAAATGCTTCACTT+TGG - chr2_2:8049710-8049729 Msa0235210:CDS 35.0%
CAGCACTAATTCATGCAATT+TGG + chr2_2:8050356-8050375 None:intergenic 35.0%
CTTTATAGGTCTCTCTTGTA+CGG - chr2_2:8050444-8050463 Msa0235210:CDS 35.0%
!!! ACCACTGATTTTGACTTTAG+GGG - chr2_2:8050740-8050759 Msa0235210:intron 35.0%
!!! TTTTGACTTTAGGGGGAATA+AGG - chr2_2:8050748-8050767 Msa0235210:intron 35.0%
ATACAAAGTGGTTATGGGTT+TGG - chr2_2:8050784-8050803 Msa0235210:intron 35.0%
CACACACGTTAGATAAAGAA+AGG + chr2_2:8051446-8051465 None:intergenic 35.0%
TCTACAAGGTCAATTGTCTT+AGG + chr2_2:8051524-8051543 None:intergenic 35.0%
! TTATGGTTGACTCATCTACA+AGG + chr2_2:8051538-8051557 None:intergenic 35.0%
TAACTCTGCAAACTCACTTA+TGG + chr2_2:8051555-8051574 None:intergenic 35.0%
! CTAATGAAGGAAAAGGGTAT+TGG + chr2_2:8051607-8051626 None:intergenic 35.0%
CATTGTTGAAAGGTTGTACA+AGG + chr2_2:8051702-8051721 None:intergenic 35.0%
!! TGCTACTTTAGGTAACTTGT+TGG - chr2_2:8051811-8051830 Msa0235210:intron 35.0%
!! TAACTTGTTGGTCACGTTAA+TGG - chr2_2:8051823-8051842 Msa0235210:intron 35.0%
!! AACTTGTTGGTCACGTTAAT+GGG - chr2_2:8051824-8051843 Msa0235210:intron 35.0%
AGCAATAGAGAACAACTCAA+TGG - chr2_2:8051853-8051872 Msa0235210:intron 35.0%
ACCAAAATGTCAAGAGTTGT+CGG - chr2_2:8051916-8051935 Msa0235210:intron 35.0%
! ATTGCTCTCTTTTGAGCTAA+AGG + chr2_2:8052046-8052065 None:intergenic 35.0%
GAGCAATGAAACTATTGCAA+TGG - chr2_2:8052059-8052078 Msa0235210:intron 35.0%
GGATGAAAGACAAGTAAAGA+TGG - chr2_2:8052087-8052106 Msa0235210:intron 35.0%
!! ATTCCAAATCTTGGTGTTTG+TGG + chr2_2:8052127-8052146 None:intergenic 35.0%
ACAAACACCAAGATTTGGAA+TGG - chr2_2:8052126-8052145 Msa0235210:CDS 35.0%
! CAAACACCAAGATTTGGAAT+GGG - chr2_2:8052127-8052146 Msa0235210:CDS 35.0%
! AAACACCAAGATTTGGAATG+GGG - chr2_2:8052128-8052147 Msa0235210:CDS 35.0%
TCTTCAATAAGACAAGCTCT+TGG + chr2_2:8052308-8052327 None:intergenic 35.0%
! ATGTTTTGCCAAAATGGCAA+CGG + chr2_2:8052403-8052422 None:intergenic 35.0%
! CACTGTATGTTTTGCCAAAA+TGG + chr2_2:8052409-8052428 None:intergenic 35.0%
CGAAATCGACACTATAACTT+TGG + chr2_2:8052442-8052461 None:intergenic 35.0%
CGATGAAGGATTTGACATGT+TGG + chr2_2:8049441-8049460 None:intergenic 40.0%
! GCAAGTTATGGAGAAGCATA+AGG - chr2_2:8049640-8049659 Msa0235210:three_prime_UTR 40.0%
! AGTTATGGAGAAGCATAAGG+AGG - chr2_2:8049643-8049662 Msa0235210:three_prime_UTR 40.0%
! AAATGCTTCACTTTGGAAGC+TGG - chr2_2:8049717-8049736 Msa0235210:CDS 40.0%
! AATGCTTCACTTTGGAAGCT+GGG - chr2_2:8049718-8049737 Msa0235210:CDS 40.0%
CAAGAAATTGGGGATAGTTG+AGG - chr2_2:8050692-8050711 Msa0235210:intron 40.0%
!!! CCACTGATTTTGACTTTAGG+GGG - chr2_2:8050741-8050760 Msa0235210:intron 40.0%
TAGTTTGGTTGTGGTTAGTG+TGG + chr2_2:8050828-8050847 None:intergenic 40.0%
AATAGAGGCAGAGACAATCA+TGG - chr2_2:8050903-8050922 Msa0235210:intron 40.0%
! GACACTCAACTCTAAAAGCT+AGG + chr2_2:8050940-8050959 None:intergenic 40.0%
! TCATGTTTCTACTGCCTGTA+GGG + chr2_2:8050965-8050984 None:intergenic 40.0%
! TTCATGTTTCTACTGCCTGT+AGG + chr2_2:8050966-8050985 None:intergenic 40.0%
!! ACCAAAATGATGGGCTACTA+GGG - chr2_2:8051142-8051161 Msa0235210:intron 40.0%
CAAGAGCTGAACAATGCTAT+AGG + chr2_2:8051342-8051361 None:intergenic 40.0%
AACGTGTGTGTTCTTTCTTC+AGG - chr2_2:8051456-8051475 Msa0235210:intron 40.0%
GTGTTCTTTCTTCAGGAACA+AGG - chr2_2:8051463-8051482 Msa0235210:intron 40.0%
! GGCTCCTAAACATGATCTTT+TGG + chr2_2:8051681-8051700 None:intergenic 40.0%
! GCAAGCCCTTTAGTTTCTTA+TGG - chr2_2:8051731-8051750 Msa0235210:intron 40.0%
! ACCGACAACTCTTGACATTT+TGG + chr2_2:8051920-8051939 None:intergenic 40.0%
GAGATAAAATGGCACAAGCT+AGG - chr2_2:8051981-8052000 Msa0235210:intron 40.0%
ACTCAAAGGGCAAATGCAAA+TGG - chr2_2:8052241-8052260 Msa0235210:CDS 40.0%
CTCAAAGGGCAAATGCAAAT+GGG - chr2_2:8052242-8052261 Msa0235210:CDS 40.0%
TCAAAGGGCAAATGCAAATG+GGG - chr2_2:8052243-8052262 Msa0235210:CDS 40.0%
AACCAATGACTTGAGTTTGG+AGG + chr2_2:8052272-8052291 None:intergenic 40.0%
CCATGAGTTCGACATGATTA+CGG - chr2_2:8052373-8052392 Msa0235210:CDS 40.0%
GAGCAAGATTGAACAGAACC+AGG - chr2_2:8049598-8049617 Msa0235210:three_prime_UTR 45.0%
! TATGGAGAAGCATAAGGAGG+AGG - chr2_2:8049646-8049665 Msa0235210:three_prime_UTR 45.0%
!! GTTGATAACAGAACTTGGGG+TGG - chr2_2:8049805-8049824 Msa0235210:CDS 45.0%
GCCACCATAGATACCAAAAC+CGG + chr2_2:8049833-8049852 None:intergenic 45.0%
!! GCGTTGATTTACCTAGCTGA+TGG - chr2_2:8049890-8049909 Msa0235210:CDS 45.0%
ACGTGTAAGTTCCATCAGCT+AGG + chr2_2:8049904-8049923 None:intergenic 45.0%
TATAGCGAATTTCACGCACC+AGG - chr2_2:8049966-8049985 Msa0235210:CDS 45.0%
CCCCCTAAAGTCAAAATCAG+TGG + chr2_2:8050744-8050763 None:intergenic 45.0%
CAAAGTGGTTATGGGTTTGG+CGG - chr2_2:8050787-8050806 Msa0235210:intron 45.0%
AAAGTGGTTATGGGTTTGGC+GGG - chr2_2:8050788-8050807 Msa0235210:intron 45.0%
! CATGTTTCTACTGCCTGTAG+GGG + chr2_2:8050964-8050983 None:intergenic 45.0%
!! CACCAAAATGATGGGCTACT+AGG - chr2_2:8051141-8051160 Msa0235210:intron 45.0%
!! CCAAAATGATGGGCTACTAG+GGG - chr2_2:8051143-8051162 Msa0235210:intron 45.0%
TATTCCTGCCTCCACTATCA+TGG + chr2_2:8051489-8051508 None:intergenic 45.0%
GTAAAGATGGGACCACATTC+TGG - chr2_2:8052100-8052119 Msa0235210:CDS 45.0%
GGTCCACAAACACCAAGATT+TGG - chr2_2:8052121-8052140 Msa0235210:CDS 45.0%
GTCCTCCAAACTCAAGTCAT+TGG - chr2_2:8052267-8052286 Msa0235210:CDS 45.0%
CCGTAATCATGTCGAACTCA+TGG + chr2_2:8052376-8052395 None:intergenic 45.0%
TGAGTTCGACATGATTACGG+TGG - chr2_2:8052376-8052395 Msa0235210:CDS 45.0%
TTTGCAGCCACACAGAGATT+CGG + chr2_2:8052475-8052494 None:intergenic 45.0%
! TGTAGGTGCTACAGCAATTC+AGG - chr2_2:8052553-8052572 Msa0235210:exon 45.0%
!!! ATTTTAAGAAATTTTTATTT+TGG + chr2_2:8050514-8050533 None:intergenic 5.0%
! AGAAGCATAAGGAGGAGGAG+AGG - chr2_2:8049651-8049670 Msa0235210:three_prime_UTR 50.0%
GAAGCATAAGGAGGAGGAGA+GGG - chr2_2:8049652-8049671 Msa0235210:three_prime_UTR 50.0%
TGGAAGCTGGGAAGAGAAAG+AGG - chr2_2:8049730-8049749 Msa0235210:CDS 50.0%
!!! GGTGCCGGTTTTGGTATCTA+TGG - chr2_2:8049826-8049845 Msa0235210:CDS 50.0%
!! GCCGGTTTTGGTATCTATGG+TGG - chr2_2:8049829-8049848 Msa0235210:CDS 50.0%
TTGTGGCACGAGTTTGAACC+TGG + chr2_2:8049987-8050006 None:intergenic 50.0%
! TGGGGATAGTTGAGGCATCA+TGG - chr2_2:8050700-8050719 Msa0235210:intron 50.0%
! GGGGATAGTTGAGGCATCAT+GGG - chr2_2:8050701-8050720 Msa0235210:intron 50.0%
AGAGGCAGAGACAATCATGG+TGG - chr2_2:8050906-8050925 Msa0235210:intron 50.0%
GAGGCAGAGACAATCATGGT+GGG - chr2_2:8050907-8050926 Msa0235210:intron 50.0%
! CCCCTAGTAGCCCATCATTT+TGG + chr2_2:8051146-8051165 None:intergenic 50.0%
CAGGAACAAGGCCATGATAG+TGG - chr2_2:8051475-8051494 Msa0235210:intron 50.0%
!! GAACAAGGCCATGATAGTGG+AGG - chr2_2:8051478-8051497 Msa0235210:intron 50.0%
GGTGTTTGTGGACCAGAATG+TGG + chr2_2:8052115-8052134 None:intergenic 50.0%
GGTGACCCCATTCCAAATCT+TGG + chr2_2:8052136-8052155 None:intergenic 50.0%
ATCTCAGCCGAATCTCTGTG+TGG - chr2_2:8052465-8052484 Msa0235210:CDS 50.0%
!! ACAGCGCTTTCGGGTTGCTT+TGG + chr2_2:8049534-8049553 None:intergenic 55.0%
GGAGGATTCACAGCGCTTTC+GGG + chr2_2:8049543-8049562 None:intergenic 55.0%
CGGAGGATTCACAGCGCTTT+CGG + chr2_2:8049544-8049563 None:intergenic 55.0%
AACAGAACTTGGGGTGGTGC+CGG - chr2_2:8049811-8049830 Msa0235210:CDS 55.0%
GAGTTGAGTGTCTCCCCTAC+AGG - chr2_2:8050948-8050967 Msa0235210:intron 55.0%
!! AAGGCCATGATAGTGGAGGC+AGG - chr2_2:8051482-8051501 Msa0235210:intron 55.0%
!! ACGGTGGACCGTTGCCATTT+TGG - chr2_2:8052392-8052411 Msa0235210:CDS 55.0%
GCATAAGGAGGAGGAGAGGG+AGG - chr2_2:8049655-8049674 Msa0235210:three_prime_UTR 60.0%
!!! ACTTGGGGTGGTGCCGGTTT+TGG - chr2_2:8049817-8049836 Msa0235210:CDS 60.0%
! GGTATCTATGGTGGCGTCGC+AGG - chr2_2:8049838-8049857 Msa0235210:CDS 60.0%
! ATCTATGGTGGCGTCGCAGG+CGG - chr2_2:8049841-8049860 Msa0235210:CDS 60.0%
! CGGTTAGTCACCGGAGAGCA+GGG - chr2_2:8049861-8049880 Msa0235210:CDS 60.0%
TCACCGGAGAGCAGGGTTGA+CGG - chr2_2:8049868-8049887 Msa0235210:CDS 60.0%
GGAGGAGGAGAGGGAGGAAG+TGG - chr2_2:8049661-8049680 Msa0235210:three_prime_UTR 65.0%
! CGTCGCAGGCGGTTAGTCAC+CGG - chr2_2:8049852-8049871 Msa0235210:CDS 65.0%
! GCGGTTAGTCACCGGAGAGC+AGG - chr2_2:8049860-8049879 Msa0235210:CDS 65.0%
ACGCCGTCAACCCTGCTCTC+CGG + chr2_2:8049874-8049893 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 8049415 8052594 8049415 ID=Msa0235210;Name=Msa0235210
chr2_2 mRNA 8049415 8052594 8049415 ID=Msa0235210-mRNA-1;Parent=Msa0235210;Name=Msa0235210-mRNA-1;_AED=0.02;_eAED=0.02;_QI=28|1|1|1|1|1|2|270|441
chr2_2 exon 8049415 8050531 8049415 ID=Msa0235210-mRNA-1:exon:3491;Parent=Msa0235210-mRNA-1
chr2_2 exon 8052088 8052594 8052088 ID=Msa0235210-mRNA-1:exon:3490;Parent=Msa0235210-mRNA-1
chr2_2 five_prime_UTR 8052567 8052594 8052567 ID=Msa0235210-mRNA-1:five_prime_utr;Parent=Msa0235210-mRNA-1
chr2_2 CDS 8052088 8052566 8052088 ID=Msa0235210-mRNA-1:cds;Parent=Msa0235210-mRNA-1
chr2_2 CDS 8049685 8050531 8049685 ID=Msa0235210-mRNA-1:cds;Parent=Msa0235210-mRNA-1
chr2_2 three_prime_UTR 8049415 8049684 8049415 ID=Msa0235210-mRNA-1:three_prime_utr;Parent=Msa0235210-mRNA-1
Gene Sequence

>Msa0235210

ATGTCAAATCCTTCATCGTCTTCTTCTATTTGGGTACTGTCTAACATAAAGCTTCAATTCTTTACTCGTATCAAACGGTTTCTTCAATCCAAAGCAACCCGAAAGCGCTGTGAATCCTCCGATTATTTAAACAAAGAAAAAAGCAGAGCTGAACAGAGCAAGATTGAACAGAACCAGGTTGAAAAGATTGAAACAGTGCAAGTTATGGAGAAGCATAAGGAGGAGGAGAGGGAGGAAGTGGAAGAAACTGAAATTTTGCTGCAAAAGACAGTGAAAATGCTTCACTTTGGAAGCTGGGAAGAGAAAGAGGTAGCTGCAAAAGAGATTGAAAGTTTAGTTAAAGAAGATGTTAAAGTTAGAAAGTTGATAACAGAACTTGGGGTGGTGCCGGTTTTGGTATCTATGGTGGCGTCGCAGGCGGTTAGTCACCGGAGAGCAGGGTTGACGGCGTTGATTTACCTAGCTGATGGAACTTACACGAACAAGGCCATGATAGTGGAGGCAGGAATATTATCCAAATTACCTAAGACAATTGACCTTGTAGATGAGTCAACCATAAGTGAGTTTGCAGAGTTACTTTTATCATTATCATCACTTGCAAACACCCAATACCCTTTTCCTTCATTAGATTTTCTTCCATTTCTTAAAGACATTCTTAAAAAAGACATGACTTTTGATACCAAAAGATCATGTTTAGGAGCCTTGTACAACCTTTCAACAATGTTAGAAAATGCAAGCCCTTTAGTTTCTTATGGTCTTGTCCCAATTCTCTTAGAACTTTCCTTAGTCAAAGAAATTTCAGAAAAAGTTCTTGCTACTTTAGGTAACTTGTTGGTCACGTTAATGGGTAAAAAAGCAATAGAGAACAACTCAATGGTGCCACAAAATTTTGTTGAGATTTTGTCATGGGAAGATAAACCAAAATGTCAAGAGTTGTCGGTTTATATTTTGATGATTTTAGCACATCAAAGTTCATCACAAAGAGATAAAATGGCACAAGCTAGGATTGTTCCTATACTTCTTGAAGTTGTTTTATTGGGGAGTCCTTTAGCTCAAAAGAGAGCAATGAAACTATTGCAATGGTTTAAGGATGAAAGACAAGTAAAGATGGGACCACATTCTGGTCCACAAACACCAAGATTTGGAATGGGGTCACCTTTAAATCAAAGAGATACAAATGAAGGGAAAAAATTGATGAAGAGTTTGGTGAAACAAAGCTTGCATAAGAATTTGGAAATAATAACTCAAAGGGCAAATGCAAATGGGGAGTCCTCCAAACTCAAGTCATTGGTTATTAGCACAAGTTCCAAGAGCTTGTCTTATTGA
Protein sequence

>Msa0235210

MSNPSSSSSIWVLSNIKLQFFTRIKRFLQSKATRKRCESSDYLNKEKSRAEQSKIEQNQVEKIETVQVMEKHKEEEREEVEETEILLQKTVKMLHFGSWEEKEVAAKEIESLVKEDVKVRKLITELGVVPVLVSMVASQAVSHRRAGLTALIYLADGTYTNKAMIVEAGILSKLPKTIDLVDESTISEFAELLLSLSSLANTQYPFPSLDFLPFLKDILKKDMTFDTKRSCLGALYNLSTMLENASPLVSYGLVPILLELSLVKEISEKVLATLGNLLVTLMGKKAIENNSMVPQNFVEILSWEDKPKCQELSVYILMILAHQSSSQRDKMAQARIVPILLEVVLLGSPLAQKRAMKLLQWFKDERQVKMGPHSGPQTPRFGMGSPLNQRDTNEGKKLMKSLVKQSLHKNLEIITQRANANGESSKLKSLVISTSSKSLSY*