AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0273600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0273600 MtrunA17_Chr2g0283691 96.599 441 14 1 1 441 1 440 0.0 860
Msa0273600 MtrunA17_Chr3g0137061 33.159 383 226 6 65 419 90 470 5.07e-44 160
Msa0273600 MtrunA17_Chr1g0149941 34.294 347 193 8 105 419 192 535 8.83e-39 147
Msa0273600 MtrunA17_Chr8g0377151 26.984 315 219 4 61 367 402 713 7.70e-20 92.4
Msa0273600 MtrunA17_Chr3g0122721 28.382 377 246 12 49 414 392 755 3.75e-19 90.5
Msa0273600 MtrunA17_Chr1g0183781 28.269 283 198 5 87 368 440 718 8.27e-18 86.3
Msa0273600 MtrunA17_Chr1g0152561 25.342 292 212 5 81 370 55 342 2.25e-13 71.2
Msa0273600 MtrunA17_Chr7g0261371 25.524 286 208 4 87 371 514 795 1.04e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0273600 AT2G27430.1 56.393 438 171 7 11 441 14 438 8.58e-154 443
Msa0273600 AT4G31890.3 36.127 346 191 11 97 419 155 493 2.64e-42 157
Msa0273600 AT4G31890.2 36.127 346 191 11 97 419 155 493 2.64e-42 157
Msa0273600 AT4G31890.1 36.127 346 191 11 97 419 155 493 2.64e-42 157
Msa0273600 AT2G25130.1 35.276 326 190 7 108 419 132 450 7.44e-42 154
Msa0273600 AT1G67530.2 29.293 297 180 8 100 379 439 722 2.94e-18 88.2
Msa0273600 AT1G67530.1 29.293 297 180 8 100 379 439 722 2.94e-18 88.2
Msa0273600 AT1G67530.4 29.293 297 180 8 100 379 439 722 2.94e-18 88.2
Msa0273600 AT2G23140.3 25.490 306 223 5 67 371 334 635 6.61e-16 80.5
Msa0273600 AT2G23140.5 25.490 306 223 5 67 371 334 635 6.61e-16 80.5
Msa0273600 AT2G23140.2 25.490 306 223 5 67 371 523 824 1.29e-15 79.7
Msa0273600 AT2G23140.4 25.490 306 223 5 67 371 523 824 1.29e-15 79.7
Msa0273600 AT2G23140.1 25.490 306 223 5 67 371 526 827 1.48e-15 79.7
Msa0273600 AT3G54790.2 28.873 284 196 6 89 370 440 719 4.38e-14 75.1
Msa0273600 AT1G27910.1 27.445 317 215 6 73 377 404 717 5.90e-14 74.7
Msa0273600 AT3G54790.3 28.873 284 196 6 89 370 476 755 6.41e-14 74.3
Msa0273600 AT3G54790.1 28.873 284 196 6 89 370 476 755 6.41e-14 74.3
Msa0273600 AT1G24330.1 25.773 291 189 5 112 387 451 729 3.42e-13 72.4
Msa0273600 AT1G24330.2 25.773 291 189 5 112 387 451 729 3.42e-13 72.4

Find 107 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTATTGCAATGGTTTA+AGG 0.113364 2_3:-5175239 Msa0273600:CDS
GTCAATTGTCTTAGGTAATT+TGG 0.115729 2_3:+5175792 None:intergenic
TGAATATAAACGGTTGAATT+TGG 0.154165 2_3:-5174793 Msa0273600:three_prime_UTR
ACCGACAACTCTTGACATTT+TGG 0.155414 2_3:+5175388 None:intergenic
TTTGTATCTCTTTGATTTAA+AGG 0.178789 2_3:+5175151 None:intergenic
ACAAAGCTTGCATAAGAATT+TGG 0.181591 2_3:-5175095 Msa0273600:CDS
TAACTTGTTGGTCACGTTAA+TGG 0.225258 2_3:-5175482 Msa0273600:CDS
CGGAGGATTCACAGCGCTTT+CGG 0.245617 2_3:+5177967 None:intergenic
GGAGGATTCACAGCGCTTTC+GGG 0.279689 2_3:+5177968 None:intergenic
TTCATCACAAAGAGATAAAA+TGG 0.283330 2_3:-5175335 Msa0273600:CDS
GCAAGCCCTTTAGTTTCTTA+TGG 0.289989 2_3:-5175574 Msa0273600:CDS
TTCTGAAATTTCTTTGACTA+AGG 0.295883 2_3:+5175525 None:intergenic
CTTTAAATCAACAACTTTGT+AGG 0.295907 2_3:-5174769 Msa0273600:three_prime_UTR
CAAAGTTATAGCGTCGATTT+CGG 0.304348 2_3:-5174865 Msa0273600:three_prime_UTR
TGTAGGTGCTACACCAATTC+AGG 0.306517 2_3:-5174752 Msa0273600:three_prime_UTR
ACGGTGGACTGTTGCCATTT+TGG 0.315369 2_3:-5174913 Msa0273600:three_prime_UTR
AAGGAAAGTTCTAAGAGAAT+TGG 0.315481 2_3:+5175544 None:intergenic
GGCTCCTAAACATGATCTTT+TGG 0.319278 2_3:+5175627 None:intergenic
GGAGGAGGAGAGGGAGGAGG+TGG 0.327726 2_3:-5177847 Msa0273600:CDS
ATTCCAAATCTTGGTGTTTG+TGG 0.341246 2_3:+5175181 None:intergenic
TGTGCCGGTGTTGGTATCTA+TGG 0.344407 2_3:-5177682 Msa0273600:CDS
AATAACCAATGACTTGAGTT+TGG 0.347977 2_3:+5175033 None:intergenic
AACTTGTTGGTCACGTTAAT+GGG 0.351557 2_3:-5175481 Msa0273600:CDS
TCTACAAGGTCAATTGTCTT+AGG 0.352811 2_3:+5175784 None:intergenic
GGTCCACAAACACCAAGATT+TGG 0.367878 2_3:-5175184 Msa0273600:CDS
GATACCAAAAGATCATGTTT+AGG 0.369581 2_3:-5175631 Msa0273600:CDS
ACAGCGCTTTCGGGTTGCTT+TGG 0.370779 2_3:+5177977 None:intergenic
TAACTCTGCAAACTCACTTA+TGG 0.378473 2_3:+5175753 None:intergenic
TTAAGAATGTCTTTAAGAAA+TGG 0.384957 2_3:+5175670 None:intergenic
CAACTTCGAAGTCTTCAATA+AGG 0.402498 2_3:+5174989 None:intergenic
GTAAAGATGGGACCACATTC+TGG 0.405463 2_3:-5175205 Msa0273600:CDS
AGAAAATCTAATGAAGGAAA+AGG 0.408091 2_3:+5175694 None:intergenic
ACTCAAAGGGCAAATGCAAA+TGG 0.411229 2_3:-5175064 Msa0273600:CDS
CGAAATCGACGCTATAACTT+TGG 0.417512 2_3:+5174866 None:intergenic
AGATGCTTCACTTTGGAAGC+TGG 0.420867 2_3:-5177791 Msa0273600:CDS
GGTATCTATGGTGGCTTCCC+AGG 0.423850 2_3:-5177670 Msa0273600:CDS
TGCTACTTTAGGTAACTTGT+TGG 0.425049 2_3:-5175494 Msa0273600:CDS
GAAGCATAAGGAGGAGGAGA+GGG 0.427827 2_3:-5177856 Msa0273600:CDS
CTCAAAGGGCAAATGCAAAT+GGG 0.429397 2_3:-5175063 Msa0273600:CDS
CCATGAGTTCGACATGATTA+CGG 0.431320 2_3:-5174932 Msa0273600:three_prime_UTR
AGGAAAGTTCTAAGAGAATT+GGG 0.434479 2_3:+5175545 None:intergenic
GCATAAGGAGGAGGAGAGGG+AGG 0.434717 2_3:-5177853 Msa0273600:CDS
TAAGGAGGAGGAGAGGGAGG+AGG 0.439148 2_3:-5177850 Msa0273600:CDS
CATGAATATCTGAATATAAA+CGG 0.450941 2_3:-5174803 Msa0273600:three_prime_UTR
GTGGTTAGTCATCGGAGAGC+AGG 0.452074 2_3:-5177648 Msa0273600:CDS
TATGGAGAAGCATAAGGAGG+AGG 0.454646 2_3:-5177862 Msa0273600:CDS
GATGCTTCACTTTGGAAGCT+GGG 0.458178 2_3:-5177790 Msa0273600:CDS
ACCAAAATGTCAAGAGTTGT+CGG 0.458853 2_3:-5175389 Msa0273600:CDS
CTCTCCGATGACTAACCACC+TGG 0.459532 2_3:+5177652 None:intergenic
GGTGACCCCATTCCAAATCT+TGG 0.459651 2_3:+5175172 None:intergenic
AAAACAACTTCAAGAAGTAT+AGG 0.466913 2_3:+5175295 None:intergenic
GTCCTCCAAACTCAAGTCAT+TGG 0.475448 2_3:-5175038 Msa0273600:CDS
GAAAATCTAATGAAGGAAAA+GGG 0.479557 2_3:+5175695 None:intergenic
GATTGAAACAGTGCAAGTTA+TGG 0.479849 2_3:-5177880 Msa0273600:CDS
TCTTCAATAAGGCAAGCTCT+TGG 0.481042 2_3:+5175000 None:intergenic
AATCAAAGAGATACAAATGA+AGG 0.481857 2_3:-5175145 Msa0273600:CDS
GAAAGTTGATAACAGAACTT+GGG 0.485027 2_3:-5177707 Msa0273600:CDS
TTTGGAAATAATAACTCAAA+GGG 0.489930 2_3:-5175077 Msa0273600:CDS
CATTGTTGAAAGGTTGTACA+AGG 0.492312 2_3:+5175606 None:intergenic
GGATGAAAGACAAGTAAAGA+TGG 0.494341 2_3:-5175218 Msa0273600:CDS
AGAAAGTTGATAACAGAACT+TGG 0.496648 2_3:-5177708 Msa0273600:CDS
CAAGACCATAAGAAACTAAA+GGG 0.497099 2_3:+5175569 None:intergenic
TTTGCAGCCACACAGAGAAT+CGG 0.497423 2_3:+5174833 None:intergenic
CAGGAACAAGGCTATGATAG+TGG 0.505796 2_3:-5175830 Msa0273600:CDS
AACCAATGACTTGAGTTTGG+AGG 0.512382 2_3:+5175036 None:intergenic
CAAACACCAAGATTTGGAAT+GGG 0.513089 2_3:-5175178 Msa0273600:CDS
CTTTGGAAGCTGGGAAGAGA+AGG 0.513641 2_3:-5177781 Msa0273600:CDS
GCAAGTTATGGAGAAGCATA+AGG 0.517239 2_3:-5177868 Msa0273600:CDS
GAGATAAAATGGCACAAGCT+AGG 0.518471 2_3:-5175324 Msa0273600:CDS
AATTCTTTACTCGTATCAAA+CGG 0.519898 2_3:-5178010 Msa0273600:CDS
AATGGAAGAAAATCTAATGA+AGG 0.520831 2_3:+5175688 None:intergenic
ACAGTGAAGATGCTTCACTT+TGG 0.523676 2_3:-5177798 Msa0273600:CDS
CGATGAAGGATTTGACATGT+TGG 0.532298 2_3:+5178070 None:intergenic
ACAAGACCATAAGAAACTAA+AGG 0.539120 2_3:+5175568 None:intergenic
ACAAACACCAAGATTTGGAA+TGG 0.539160 2_3:-5175179 Msa0273600:CDS
AACAGAACTTGGGGTTGTGC+CGG 0.544571 2_3:-5177697 Msa0273600:CDS
GAGCAATGAAACTATTGCAA+TGG 0.549239 2_3:-5175246 Msa0273600:CDS
AGAAGCATAAGGAGGAGGAG+AGG 0.553180 2_3:-5177857 Msa0273600:CDS
ACGTTGATTTACCTAGCTGA+TGG 0.553971 2_3:-5177618 Msa0273600:CDS
GTATTCTTTCTTCAGGAACA+AGG 0.556956 2_3:-5175842 Msa0273600:intron
ACTTGGGGTTGTGCCGGTGT+TGG 0.560426 2_3:-5177691 Msa0273600:CDS
CCGTAATCATGTCGAACTCA+TGG 0.560647 2_3:+5174932 None:intergenic
GATGAAAGACAAGTAAAGAT+GGG 0.560923 2_3:-5175217 Msa0273600:CDS
TTCTTTGTTTAAATAATCGG+AGG 0.562901 2_3:+5177950 None:intergenic
ATTTGGAAATAATAACTCAA+AGG 0.567717 2_3:-5175078 Msa0273600:CDS
AGCAATAGAGAACAACTCAA+TGG 0.577734 2_3:-5175452 Msa0273600:CDS
CTTCCCAGGTGGTTAGTCAT+CGG 0.577768 2_3:-5177656 Msa0273600:CDS
TGGAAGCTGGGAAGAGAAGG+AGG 0.583399 2_3:-5177778 Msa0273600:CDS
GGTGTTTGTGGACCAGAATG+TGG 0.586193 2_3:+5175193 None:intergenic
AAGGCTATGATAGTGGAGGC+AGG 0.586256 2_3:-5175823 Msa0273600:CDS
ATCAAAGAGATACAAATGAA+GGG 0.587935 2_3:-5175144 Msa0273600:CDS
ATGGTCAATTCAAACATACA+TGG 0.591645 2_3:+5174951 None:intergenic
ATCTATGGTGGCTTCCCAGG+TGG 0.600429 2_3:-5177667 Msa0273600:CDS
TGGTTAGTCATCGGAGAGCA+GGG 0.605588 2_3:-5177647 Msa0273600:CDS
ATCTCAGCCGATTCTCTGTG+TGG 0.614183 2_3:-5174840 Msa0273600:three_prime_UTR
TCAAAGGGCAAATGCAAATG+GGG 0.635611 2_3:-5175062 Msa0273600:CDS
TTATGGTTGACTCATCTACA+AGG 0.635954 2_3:+5175770 None:intergenic
ACGTGTAAGTTCCATCAGCT+AGG 0.637555 2_3:+5177607 None:intergenic
GAGCAAGATTGAACAGAACC+AGG 0.641982 2_3:-5177910 Msa0273600:CDS
GCCGGTGTTGGTATCTATGG+TGG 0.644634 2_3:-5177679 Msa0273600:CDS
AAAGTTGATAACAGAACTTG+GGG 0.647620 2_3:-5177706 Msa0273600:CDS
AAACACCAAGATTTGGAATG+GGG 0.656392 2_3:-5175177 Msa0273600:CDS
GAACAAGGCTATGATAGTGG+AGG 0.667903 2_3:-5175827 Msa0273600:CDS
AGTTATGGAGAAGCATAAGG+AGG 0.680310 2_3:-5177865 Msa0273600:CDS
GCCACCATAGATACCAACAC+CGG 0.692048 2_3:+5177678 None:intergenic
TGAGTTCGACATGATTACGG+TGG 0.717362 2_3:-5174929 Msa0273600:three_prime_UTR
TCTCCGATGACTAACCACCT+GGG 0.727974 2_3:+5177653 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCTTTGTTTAAATAAT+CGG + chr2_3:5174882-5174901 None:intergenic 10.0%
!! TTGTATAAAATATAATTAAG+TGG + chr2_3:5176134-5176153 None:intergenic 10.0%
!!! TTATATTTTATACAAGAAAT+TGG - chr2_3:5176139-5176158 Msa0273600:intron 10.0%
!! AATAAAATTAACATTTCAAA+TGG - chr2_3:5176378-5176397 Msa0273600:intron 10.0%
!! TTAATGCAATTTAATTAATT+TGG + chr2_3:5176486-5176505 None:intergenic 10.0%
!! TATTTAAACAAAGAAAAAAG+CGG - chr2_3:5174884-5174903 Msa0273600:three_prime_UTR 15.0%
!!! CTAAAAAACATTGTAATTTT+AGG + chr2_3:5175419-5175438 None:intergenic 15.0%
!! ATGTTATTCCAAAATTATTA+CGG - chr2_3:5176066-5176085 Msa0273600:intron 15.0%
!! ACATTAAATAAAAAACAGTT+TGG + chr2_3:5176302-5176321 None:intergenic 15.0%
!! GAAAAAAAAAATTAATGTGT+TGG + chr2_3:5176800-5176819 None:intergenic 15.0%
!! TTTAAAGAGTCTAAATTGTT+GGG - chr2_3:5175624-5175643 Msa0273600:CDS 20.0%
!! CATAGTAGATGTTTATTTAA+TGG - chr2_3:5175674-5175693 Msa0273600:CDS 20.0%
!!! GATATAAACCGTAATAATTT+TGG + chr2_3:5176077-5176096 None:intergenic 20.0%
!! CAAGATTTATTAATACAAAG+TGG - chr2_3:5176231-5176250 Msa0273600:intron 20.0%
!! TATTAATACAAAGTGGTTAT+TGG - chr2_3:5176238-5176257 Msa0273600:intron 20.0%
!! AAAATTAACATTTCAAATGG+AGG - chr2_3:5176381-5176400 Msa0273600:intron 20.0%
!!! TTAATTTGATGCCAAAATAT+TGG + chr2_3:5176537-5176556 None:intergenic 20.0%
!! ATATTAAATCACCAAAATGA+TGG - chr2_3:5176627-5176646 Msa0273600:intron 20.0%
!! TATTAAATCACCAAAATGAT+GGG - chr2_3:5176628-5176647 Msa0273600:intron 20.0%
!! TAATAAAATACAGTATAGGT+AGG - chr2_3:5176711-5176730 Msa0273600:intron 20.0%
!!! TTAAGAATGTCTTTAAGAAA+TGG + chr2_3:5177159-5177178 None:intergenic 20.0%
!!! AAAATCTCAACAAAATTTTG+TGG + chr2_3:5177402-5177421 None:intergenic 20.0%
!!! TTTGTATCTCTTTGATTTAA+AGG + chr2_3:5177678-5177697 None:intergenic 20.0%
!! ATTTGGAAATAATAACTCAA+AGG - chr2_3:5177748-5177767 Msa0273600:CDS 20.0%
!! TTTGGAAATAATAACTCAAA+GGG - chr2_3:5177749-5177768 Msa0273600:CDS 20.0%
!! CATGAATATCTGAATATAAA+CGG - chr2_3:5178023-5178042 Msa0273600:CDS 20.0%
!!! AATACTTTAAAAACCTGAAT+TGG + chr2_3:5178090-5178109 None:intergenic 20.0%
! AATTCTTTACTCGTATCAAA+CGG - chr2_3:5174816-5174835 Msa0273600:three_prime_UTR 25.0%
!! TTCTTTGTTTAAATAATCGG+AGG + chr2_3:5174879-5174898 None:intergenic 25.0%
! TAGAGACATTTCATATATTG+TGG + chr2_3:5175321-5175340 None:intergenic 25.0%
! ATGTGTTCTTAAGATGTTAT+TGG - chr2_3:5175580-5175599 Msa0273600:CDS 25.0%
! TGGTTAAAATGTTGAATTGA+TGG - chr2_3:5175600-5175619 Msa0273600:CDS 25.0%
! GGTTAAAATGTTGAATTGAT+GGG - chr2_3:5175601-5175620 Msa0273600:CDS 25.0%
! GTTTAAAGAGTCTAAATTGT+TGG - chr2_3:5175623-5175642 Msa0273600:CDS 25.0%
! TCTACTATGAATATGAACAT+AGG + chr2_3:5175663-5175682 None:intergenic 25.0%
! AATAAAAAACAGTTTGGTTG+TGG + chr2_3:5176296-5176315 None:intergenic 25.0%
!! ATGGAATTTAGCCAATATTT+TGG - chr2_3:5176523-5176542 Msa0273600:intron 25.0%
! TTTCTATGTGAAGTGTATTA+TGG + chr2_3:5176585-5176604 None:intergenic 25.0%
! CTGCTAATAAAATACAGTAT+AGG - chr2_3:5176707-5176726 Msa0273600:intron 25.0%
! GAAAATCTAATGAAGGAAAA+GGG + chr2_3:5177134-5177153 None:intergenic 25.0%
! AGAAAATCTAATGAAGGAAA+AGG + chr2_3:5177135-5177154 None:intergenic 25.0%
! AATGGAAGAAAATCTAATGA+AGG + chr2_3:5177141-5177160 None:intergenic 25.0%
!! CATTTTCTAACATTGTTGAA+AGG + chr2_3:5177233-5177252 None:intergenic 25.0%
!!! TTCTGAAATTTCTTTGACTA+AGG + chr2_3:5177304-5177323 None:intergenic 25.0%
!! ATTTTGTTGAGATTTTGTCA+TGG - chr2_3:5177406-5177425 Msa0273600:intron 25.0%
!! TTTTGTTGAGATTTTGTCAT+GGG - chr2_3:5177407-5177426 Msa0273600:intron 25.0%
! TTCATCACAAAGAGATAAAA+TGG - chr2_3:5177491-5177510 Msa0273600:intron 25.0%
!! AAAACAACTTCAAGAAGTAT+AGG + chr2_3:5177534-5177553 None:intergenic 25.0%
!!! ACTTCTTGAAGTTGTTTTAT+TGG - chr2_3:5177536-5177555 Msa0273600:intron 25.0%
!!! CTTCTTGAAGTTGTTTTATT+GGG - chr2_3:5177537-5177556 Msa0273600:intron 25.0%
!!! TTCTTGAAGTTGTTTTATTG+GGG - chr2_3:5177538-5177557 Msa0273600:intron 25.0%
! AATCAAAGAGATACAAATGA+AGG - chr2_3:5177681-5177700 Msa0273600:CDS 25.0%
! ATCAAAGAGATACAAATGAA+GGG - chr2_3:5177682-5177701 Msa0273600:CDS 25.0%
! GAAAAAATTGATGAAGAGTT+TGG - chr2_3:5177704-5177723 Msa0273600:CDS 25.0%
! TGAATATAAACGGTTGAATT+TGG - chr2_3:5178033-5178052 Msa0273600:CDS 25.0%
! CTTTAAATCAACAACTTTGT+AGG - chr2_3:5178057-5178076 Msa0273600:CDS 25.0%
! TTCATCGTCTTCTTCTTTTT+GGG - chr2_3:5174772-5174791 Msa0273600:three_prime_UTR 30.0%
!! AGAAAGTTGATAACAGAACT+TGG - chr2_3:5175118-5175137 Msa0273600:CDS 30.0%
!! GAAAGTTGATAACAGAACTT+GGG - chr2_3:5175119-5175138 Msa0273600:CDS 30.0%
!! AAAGTTGATAACAGAACTTG+GGG - chr2_3:5175120-5175139 Msa0273600:CDS 30.0%
!!! TAACCACTGATTTTGACTTT+CGG - chr2_3:5176197-5176216 Msa0273600:intron 30.0%
! ATACAAAGTGGTTATTGGTT+TGG - chr2_3:5176243-5176262 Msa0273600:intron 30.0%
GAATTTGAAGAACTACACTT+TGG + chr2_3:5176340-5176359 None:intergenic 30.0%
AATGACATCAAATCTGATGA+TGG - chr2_3:5176504-5176523 Msa0273600:intron 30.0%
ACTTCACATAGAAATTGAGT+TGG - chr2_3:5176591-5176610 Msa0273600:intron 30.0%
! GTATTAACTTGGTCAAAGTT+TGG + chr2_3:5176936-5176955 None:intergenic 30.0%
! AAGGAAAGCATGTATTAACT+TGG + chr2_3:5176947-5176966 None:intergenic 30.0%
GTCAATTGTCTTAGGTAATT+TGG + chr2_3:5177037-5177056 None:intergenic 30.0%
GATACCAAAAGATCATGTTT+AGG - chr2_3:5177195-5177214 Msa0273600:intron 30.0%
CAAGACCATAAGAAACTAAA+GGG + chr2_3:5177260-5177279 None:intergenic 30.0%
ACAAGACCATAAGAAACTAA+AGG + chr2_3:5177261-5177280 None:intergenic 30.0%
!! AGGAAAGTTCTAAGAGAATT+GGG + chr2_3:5177284-5177303 None:intergenic 30.0%
!! AAGGAAAGTTCTAAGAGAAT+TGG + chr2_3:5177285-5177304 None:intergenic 30.0%
GAAACTATTGCAATGGTTTA+AGG - chr2_3:5177587-5177606 Msa0273600:intron 30.0%
GATGAAAGACAAGTAAAGAT+GGG - chr2_3:5177609-5177628 Msa0273600:CDS 30.0%
ACAAAGCTTGCATAAGAATT+TGG - chr2_3:5177731-5177750 Msa0273600:CDS 30.0%
AATAACCAATGACTTGAGTT+TGG + chr2_3:5177796-5177815 None:intergenic 30.0%
ATGGTCAATTCAAACATACA+TGG + chr2_3:5177878-5177897 None:intergenic 30.0%
CAAAAAGAAGAAGACGATGA+AGG + chr2_3:5174773-5174792 None:intergenic 35.0%
! CTTCATCGTCTTCTTCTTTT+TGG - chr2_3:5174771-5174790 Msa0273600:three_prime_UTR 35.0%
! CTGTTTCAATCTTTTCAACC+TGG + chr2_3:5174937-5174956 None:intergenic 35.0%
GATTGAAACAGTGCAAGTTA+TGG - chr2_3:5174946-5174965 Msa0273600:three_prime_UTR 35.0%
! CAAGAAATTGGTGATAGTTG+AGG - chr2_3:5176151-5176170 Msa0273600:intron 35.0%
!!! AACCACTGATTTTGACTTTC+GGG - chr2_3:5176198-5176217 Msa0273600:intron 35.0%
ACACACGTTAGGTATAAAGA+AGG + chr2_3:5176966-5176985 None:intergenic 35.0%
GAAGAAAGAATACACACGTT+AGG + chr2_3:5176977-5176996 None:intergenic 35.0%
AACGTGTGTATTCTTTCTTC+AGG - chr2_3:5176977-5176996 Msa0273600:intron 35.0%
GTATTCTTTCTTCAGGAACA+AGG - chr2_3:5176984-5177003 Msa0273600:intron 35.0%
TCTACAAGGTCAATTGTCTT+AGG + chr2_3:5177045-5177064 None:intergenic 35.0%
! TTATGGTTGACTCATCTACA+AGG + chr2_3:5177059-5177078 None:intergenic 35.0%
TAACTCTGCAAACTCACTTA+TGG + chr2_3:5177076-5177095 None:intergenic 35.0%
CATTGTTGAAAGGTTGTACA+AGG + chr2_3:5177223-5177242 None:intergenic 35.0%
!! GAAAAAGCTCTTGCTACTTT+AGG - chr2_3:5177321-5177340 Msa0273600:intron 35.0%
!! TGCTACTTTAGGTAACTTGT+TGG - chr2_3:5177332-5177351 Msa0273600:intron 35.0%
!! TAACTTGTTGGTCACGTTAA+TGG - chr2_3:5177344-5177363 Msa0273600:intron 35.0%
!! AACTTGTTGGTCACGTTAAT+GGG - chr2_3:5177345-5177364 Msa0273600:intron 35.0%
AGCAATAGAGAACAACTCAA+TGG - chr2_3:5177374-5177393 Msa0273600:intron 35.0%
ACCAAAATGTCAAGAGTTGT+CGG - chr2_3:5177437-5177456 Msa0273600:intron 35.0%
! ATTGCTCTCTTTTGAGCTAA+AGG + chr2_3:5177567-5177586 None:intergenic 35.0%
GAGCAATGAAACTATTGCAA+TGG - chr2_3:5177580-5177599 Msa0273600:intron 35.0%
GGATGAAAGACAAGTAAAGA+TGG - chr2_3:5177608-5177627 Msa0273600:intron 35.0%
!! ATTCCAAATCTTGGTGTTTG+TGG + chr2_3:5177648-5177667 None:intergenic 35.0%
ACAAACACCAAGATTTGGAA+TGG - chr2_3:5177647-5177666 Msa0273600:CDS 35.0%
! CAAACACCAAGATTTGGAAT+GGG - chr2_3:5177648-5177667 Msa0273600:CDS 35.0%
! AAACACCAAGATTTGGAATG+GGG - chr2_3:5177649-5177668 Msa0273600:CDS 35.0%
CAACTTCGAAGTCTTCAATA+AGG + chr2_3:5177840-5177859 None:intergenic 35.0%
! CACTGTATGTTTTGCCAAAA+TGG + chr2_3:5177930-5177949 None:intergenic 35.0%
!! CAAAGTTATAGCGTCGATTT+CGG - chr2_3:5177961-5177980 Msa0273600:CDS 35.0%
CGATGAAGGATTTGACATGT+TGG + chr2_3:5174759-5174778 None:intergenic 40.0%
! GCAAGTTATGGAGAAGCATA+AGG - chr2_3:5174958-5174977 Msa0273600:three_prime_UTR 40.0%
! AGTTATGGAGAAGCATAAGG+AGG - chr2_3:5174961-5174980 Msa0273600:three_prime_UTR 40.0%
ACAGTGAAGATGCTTCACTT+TGG - chr2_3:5175028-5175047 Msa0273600:CDS 40.0%
!! ACGTTGATTTACCTAGCTGA+TGG - chr2_3:5175208-5175227 Msa0273600:CDS 40.0%
!!! ACCACTGATTTTGACTTTCG+GGG - chr2_3:5176199-5176218 Msa0273600:intron 40.0%
!!! TTTTGACTTTCGGGGCAATA+AGG - chr2_3:5176207-5176226 Msa0273600:intron 40.0%
!! CAAAGTGGTTATTGGTTTGG+CGG - chr2_3:5176246-5176265 Msa0273600:intron 40.0%
!! AAAGTGGTTATTGGTTTGGC+GGG - chr2_3:5176247-5176266 Msa0273600:intron 40.0%
TACACTTTGGTTGTGACTTG+TGG + chr2_3:5176327-5176346 None:intergenic 40.0%
!! ACCAAAATGATGGGCTACTA+GGG - chr2_3:5176637-5176656 Msa0273600:intron 40.0%
CAAGAGCTGAACAATGCTAT+AGG + chr2_3:5176867-5176886 None:intergenic 40.0%
! GGCTCCTAAACATGATCTTT+TGG + chr2_3:5177202-5177221 None:intergenic 40.0%
! GCAAGCCCTTTAGTTTCTTA+TGG - chr2_3:5177252-5177271 Msa0273600:intron 40.0%
! ACCGACAACTCTTGACATTT+TGG + chr2_3:5177441-5177460 None:intergenic 40.0%
GAGATAAAATGGCACAAGCT+AGG - chr2_3:5177502-5177521 Msa0273600:intron 40.0%
ACTCAAAGGGCAAATGCAAA+TGG - chr2_3:5177762-5177781 Msa0273600:CDS 40.0%
CTCAAAGGGCAAATGCAAAT+GGG - chr2_3:5177763-5177782 Msa0273600:CDS 40.0%
TCAAAGGGCAAATGCAAATG+GGG - chr2_3:5177764-5177783 Msa0273600:CDS 40.0%
AACCAATGACTTGAGTTTGG+AGG + chr2_3:5177793-5177812 None:intergenic 40.0%
TCTTCAATAAGGCAAGCTCT+TGG + chr2_3:5177829-5177848 None:intergenic 40.0%
CCATGAGTTCGACATGATTA+CGG - chr2_3:5177894-5177913 Msa0273600:CDS 40.0%
CGAAATCGACGCTATAACTT+TGG + chr2_3:5177963-5177982 None:intergenic 40.0%
GAGCAAGATTGAACAGAACC+AGG - chr2_3:5174916-5174935 Msa0273600:three_prime_UTR 45.0%
! TATGGAGAAGCATAAGGAGG+AGG - chr2_3:5174964-5174983 Msa0273600:three_prime_UTR 45.0%
! AGATGCTTCACTTTGGAAGC+TGG - chr2_3:5175035-5175054 Msa0273600:CDS 45.0%
! GATGCTTCACTTTGGAAGCT+GGG - chr2_3:5175036-5175055 Msa0273600:CDS 45.0%
ACGTGTAAGTTCCATCAGCT+AGG + chr2_3:5175222-5175241 None:intergenic 45.0%
! TGGTGATAGTTGAGGCATCA+TGG - chr2_3:5176159-5176178 Msa0273600:intron 45.0%
! GGTGATAGTTGAGGCATCAT+GGG - chr2_3:5176160-5176179 Msa0273600:intron 45.0%
CAGTTTGGTTGTGGTTAGTG+TGG + chr2_3:5176287-5176306 None:intergenic 45.0%
AATGGAGGCAGAGAGAATCA+TGG - chr2_3:5176396-5176415 Msa0273600:intron 45.0%
TTCACGTTCTACTGCCTGTA+GGG + chr2_3:5176458-5176477 None:intergenic 45.0%
TTTCACGTTCTACTGCCTGT+AGG + chr2_3:5176459-5176478 None:intergenic 45.0%
!! CACCAAAATGATGGGCTACT+AGG - chr2_3:5176636-5176655 Msa0273600:intron 45.0%
!! CCAAAATGATGGGCTACTAG+GGG - chr2_3:5176638-5176657 Msa0273600:intron 45.0%
CAGGAACAAGGCTATGATAG+TGG - chr2_3:5176996-5177015 Msa0273600:intron 45.0%
!! GAACAAGGCTATGATAGTGG+AGG - chr2_3:5176999-5177018 Msa0273600:intron 45.0%
GTAAAGATGGGACCACATTC+TGG - chr2_3:5177621-5177640 Msa0273600:CDS 45.0%
GGTCCACAAACACCAAGATT+TGG - chr2_3:5177642-5177661 Msa0273600:CDS 45.0%
GTCCTCCAAACTCAAGTCAT+TGG - chr2_3:5177788-5177807 Msa0273600:CDS 45.0%
CCGTAATCATGTCGAACTCA+TGG + chr2_3:5177897-5177916 None:intergenic 45.0%
TGAGTTCGACATGATTACGG+TGG - chr2_3:5177897-5177916 Msa0273600:CDS 45.0%
TTTGCAGCCACACAGAGAAT+CGG + chr2_3:5177996-5178015 None:intergenic 45.0%
! TGTAGGTGCTACACCAATTC+AGG - chr2_3:5178074-5178093 Msa0273600:exon 45.0%
!!! AAATAATTTATTTTTTTGAA+AGG - chr2_3:5175947-5175966 Msa0273600:intron 5.0%
! AGAAGCATAAGGAGGAGGAG+AGG - chr2_3:5174969-5174988 Msa0273600:three_prime_UTR 50.0%
GAAGCATAAGGAGGAGGAGA+GGG - chr2_3:5174970-5174989 Msa0273600:three_prime_UTR 50.0%
! CTTTGGAAGCTGGGAAGAGA+AGG - chr2_3:5175045-5175064 Msa0273600:CDS 50.0%
AACAGAACTTGGGGTTGTGC+CGG - chr2_3:5175129-5175148 Msa0273600:CDS 50.0%
!! TGTGCCGGTGTTGGTATCTA+TGG - chr2_3:5175144-5175163 Msa0273600:CDS 50.0%
GCCACCATAGATACCAACAC+CGG + chr2_3:5175151-5175170 None:intergenic 50.0%
! CTTCCCAGGTGGTTAGTCAT+CGG - chr2_3:5175170-5175189 Msa0273600:CDS 50.0%
TCTCCGATGACTAACCACCT+GGG + chr2_3:5175176-5175195 None:intergenic 50.0%
! TGGTTAGTCATCGGAGAGCA+GGG - chr2_3:5175179-5175198 Msa0273600:CDS 50.0%
ATTGTGGCACGAGCTTGAAC+TGG + chr2_3:5175305-5175324 None:intergenic 50.0%
GCCCCGAAAGTCAAAATCAG+TGG + chr2_3:5176203-5176222 None:intergenic 50.0%
GAGGCAGAGAGAATCATGGT+GGG - chr2_3:5176400-5176419 Msa0273600:intron 50.0%
TCACGTTCTACTGCCTGTAG+GGG + chr2_3:5176457-5176476 None:intergenic 50.0%
! CCCCTAGTAGCCCATCATTT+TGG + chr2_3:5176641-5176660 None:intergenic 50.0%
!! AAGGCTATGATAGTGGAGGC+AGG - chr2_3:5177003-5177022 Msa0273600:intron 50.0%
GGTGTTTGTGGACCAGAATG+TGG + chr2_3:5177636-5177655 None:intergenic 50.0%
GGTGACCCCATTCCAAATCT+TGG + chr2_3:5177657-5177676 None:intergenic 50.0%
!!! ACGGTGGACTGTTGCCATTT+TGG - chr2_3:5177913-5177932 Msa0273600:CDS 50.0%
! ATCTCAGCCGATTCTCTGTG+TGG - chr2_3:5177986-5178005 Msa0273600:CDS 50.0%
!! ACAGCGCTTTCGGGTTGCTT+TGG + chr2_3:5174852-5174871 None:intergenic 55.0%
GGAGGATTCACAGCGCTTTC+GGG + chr2_3:5174861-5174880 None:intergenic 55.0%
CGGAGGATTCACAGCGCTTT+CGG + chr2_3:5174862-5174881 None:intergenic 55.0%
TGGAAGCTGGGAAGAGAAGG+AGG - chr2_3:5175048-5175067 Msa0273600:CDS 55.0%
!! GCCGGTGTTGGTATCTATGG+TGG - chr2_3:5175147-5175166 Msa0273600:CDS 55.0%
GGTATCTATGGTGGCTTCCC+AGG - chr2_3:5175156-5175175 Msa0273600:CDS 55.0%
ATCTATGGTGGCTTCCCAGG+TGG - chr2_3:5175159-5175178 Msa0273600:CDS 55.0%
CTCTCCGATGACTAACCACC+TGG + chr2_3:5175177-5175196 None:intergenic 55.0%
! GTGGTTAGTCATCGGAGAGC+AGG - chr2_3:5175178-5175197 Msa0273600:CDS 55.0%
GGAGGCAGAGAGAATCATGG+TGG - chr2_3:5176399-5176418 Msa0273600:intron 55.0%
GAGTTGAGTGTCTCCCCTAC+AGG - chr2_3:5176441-5176460 Msa0273600:intron 55.0%
GCATAAGGAGGAGGAGAGGG+AGG - chr2_3:5174973-5174992 Msa0273600:three_prime_UTR 60.0%
TAAGGAGGAGGAGAGGGAGG+AGG - chr2_3:5174976-5174995 Msa0273600:three_prime_UTR 60.0%
! ACTTGGGGTTGTGCCGGTGT+TGG - chr2_3:5175135-5175154 Msa0273600:CDS 60.0%
GGAGGAGGAGAGGGAGGAGG+TGG - chr2_3:5174979-5174998 Msa0273600:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 5174733 5178115 5174733 ID=Msa0273600;Name=Msa0273600
chr2_3 mRNA 5174733 5178115 5174733 ID=Msa0273600-mRNA-1;Parent=Msa0273600;Name=Msa0273600-mRNA-1;_AED=0.00;_eAED=0.00;_QI=28|1|1|1|1|1|2|270|441
chr2_3 exon 5174733 5175849 5174733 ID=Msa0273600-mRNA-1:exon:2511;Parent=Msa0273600-mRNA-1
chr2_3 exon 5177609 5178115 5177609 ID=Msa0273600-mRNA-1:exon:2510;Parent=Msa0273600-mRNA-1
chr2_3 five_prime_UTR 5178088 5178115 5178088 ID=Msa0273600-mRNA-1:five_prime_utr;Parent=Msa0273600-mRNA-1
chr2_3 CDS 5177609 5178087 5177609 ID=Msa0273600-mRNA-1:cds;Parent=Msa0273600-mRNA-1
chr2_3 CDS 5175003 5175849 5175003 ID=Msa0273600-mRNA-1:cds;Parent=Msa0273600-mRNA-1
chr2_3 three_prime_UTR 5174733 5175002 5174733 ID=Msa0273600-mRNA-1:three_prime_utr;Parent=Msa0273600-mRNA-1
Gene Sequence

>Msa0273600

ATGTCAAATCCTTCATCGTCTTCTTCTTTTTGGGTACTGTCTAACATAAAGCTTCAATTCTTTACTCGTATCAAACGGTTTCTTCAATCCAAAGCAACCCGAAAGCGCTGTGAATCCTCCGATTATTTAAACAAAGAAAAAAGCGGAGCTGAACAGAGCAAGATTGAACAGAACCAGGTTGAAAAGATTGAAACAGTGCAAGTTATGGAGAAGCATAAGGAGGAGGAGAGGGAGGAGGTGGAAGAAACTGAAATTTTGTTGCAAAAGACAGTGAAGATGCTTCACTTTGGAAGCTGGGAAGAGAAGGAGGTAGCTGCAAAAGAGATTGAAAGTTTAGTTAAACAAGATGTTAAAGTAAGAAAGTTGATAACAGAACTTGGGGTTGTGCCGGTGTTGGTATCTATGGTGGCTTCCCAGGTGGTTAGTCATCGGAGAGCAGGGTTGACGACGTTGATTTACCTAGCTGATGGAACTTACACGAACAAGGCTATGATAGTGGAGGCAGGAATATTATCCAAATTACCTAAGACAATTGACCTTGTAGATGAGTCAACCATAAGTGAGTTTGCAGAGTTACTTTTATCATTATCATCACTTGCAAACACACAATACCCTTTTCCTTCATTAGATTTTCTTCCATTTCTTAAAGACATTCTTAAAAAAGACATGACTTTTGATACCAAAAGATCATGTTTAGGAGCCTTGTACAACCTTTCAACAATGTTAGAAAATGCAAGCCCTTTAGTTTCTTATGGTCTTGTCCCAATTCTCTTAGAACTTTCCTTAGTCAAAGAAATTTCAGAAAAAGCTCTTGCTACTTTAGGTAACTTGTTGGTCACGTTAATGGGTAAAAAAGCAATAGAGAACAACTCAATGGTGCCACAAAATTTTGTTGAGATTTTGTCATGGGAAGATAAACCAAAATGTCAAGAGTTGTCGGTTTATATTTTGATGATTTTAGCACATCAAAGTTCATCACAAAGAGATAAAATGGCACAAGCTAGGATTGTTCCTATACTTCTTGAAGTTGTTTTATTGGGGAGTCCTTTAGCTCAAAAGAGAGCAATGAAACTATTGCAATGGTTTAAGGATGAAAGACAAGTAAAGATGGGACCACATTCTGGTCCACAAACACCAAGATTTGGAATGGGGTCACCTTTAAATCAAAGAGATACAAATGAAGGGAAAAAATTGATGAAGAGTTTGGTGAAACAAAGCTTGCATAAGAATTTGGAAATAATAACTCAAAGGGCAAATGCAAATGGGGAGTCCTCCAAACTCAAGTCATTGGTTATTAGCACAAGTTCCAAGAGCTTGCCTTATTGA
Protein sequence

>Msa0273600

MSNPSSSSSFWVLSNIKLQFFTRIKRFLQSKATRKRCESSDYLNKEKSGAEQSKIEQNQVEKIETVQVMEKHKEEEREEVEETEILLQKTVKMLHFGSWEEKEVAAKEIESLVKQDVKVRKLITELGVVPVLVSMVASQVVSHRRAGLTTLIYLADGTYTNKAMIVEAGILSKLPKTIDLVDESTISEFAELLLSLSSLANTQYPFPSLDFLPFLKDILKKDMTFDTKRSCLGALYNLSTMLENASPLVSYGLVPILLELSLVKEISEKALATLGNLLVTLMGKKAIENNSMVPQNFVEILSWEDKPKCQELSVYILMILAHQSSSQRDKMAQARIVPILLEVVLLGSPLAQKRAMKLLQWFKDERQVKMGPHSGPQTPRFGMGSPLNQRDTNEGKKLMKSLVKQSLHKNLEIITQRANANGESSKLKSLVISTSSKSLPY*