AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0313820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313820 MtrunA17_Chr2g0283691 96.145 441 16 1 1 441 1 440 0.0 858
Msa0313820 MtrunA17_Chr3g0137061 32.898 383 227 6 65 419 90 470 5.99e-43 157
Msa0313820 MtrunA17_Chr1g0149941 34.006 347 194 8 105 419 192 535 6.62e-38 145
Msa0313820 MtrunA17_Chr8g0377151 26.984 315 219 4 61 367 402 713 6.15e-20 92.8
Msa0313820 MtrunA17_Chr3g0122721 28.610 374 243 12 55 417 398 758 2.76e-19 90.9
Msa0313820 MtrunA17_Chr1g0183781 28.269 283 198 5 87 368 440 718 7.30e-18 86.3
Msa0313820 MtrunA17_Chr1g0152561 25.342 292 212 5 81 370 55 342 2.33e-13 70.9
Msa0313820 MtrunA17_Chr7g0261371 25.524 286 208 4 87 371 514 795 7.66e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313820 AT2G27430.1 56.720 439 170 7 10 441 13 438 4.18e-154 444
Msa0313820 AT4G31890.3 35.838 346 192 11 97 419 155 493 2.68e-41 154
Msa0313820 AT4G31890.2 35.838 346 192 11 97 419 155 493 2.68e-41 154
Msa0313820 AT4G31890.1 35.838 346 192 11 97 419 155 493 2.68e-41 154
Msa0313820 AT2G25130.1 34.969 326 191 7 108 419 132 450 7.61e-41 152
Msa0313820 AT1G67530.2 29.293 297 180 8 100 379 439 722 2.59e-18 88.2
Msa0313820 AT1G67530.1 29.293 297 180 8 100 379 439 722 2.59e-18 88.2
Msa0313820 AT1G67530.4 29.293 297 180 8 100 379 439 722 2.59e-18 88.2
Msa0313820 AT2G23140.3 25.364 343 248 7 30 371 300 635 1.75e-16 82.4
Msa0313820 AT2G23140.5 25.364 343 248 7 30 371 300 635 1.75e-16 82.4
Msa0313820 AT2G23140.2 25.364 343 248 7 30 371 489 824 2.76e-16 82.0
Msa0313820 AT2G23140.4 25.364 343 248 7 30 371 489 824 2.76e-16 82.0
Msa0313820 AT2G23140.1 25.364 343 248 7 30 371 492 827 2.94e-16 82.0
Msa0313820 AT3G54790.2 28.873 284 196 6 89 370 440 719 4.49e-14 75.1
Msa0313820 AT1G27910.1 27.445 317 215 6 73 377 404 717 5.69e-14 74.7
Msa0313820 AT3G54790.3 28.873 284 196 6 89 370 476 755 5.81e-14 74.7
Msa0313820 AT3G54790.1 28.873 284 196 6 89 370 476 755 5.81e-14 74.7
Msa0313820 AT1G24330.1 25.773 291 189 5 112 387 451 729 3.31e-13 72.4
Msa0313820 AT1G24330.2 25.773 291 189 5 112 387 451 729 3.31e-13 72.4

Find 108 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATCATCTTCTTCTATT+TGG 0.105767 2_4:-9269079 Msa0313820:CDS
GAAACTATTGCAATGGTTTA+AGG 0.113364 2_4:-9266263 Msa0313820:CDS
GTCAATTGTCTTAGGTAATT+TGG 0.115729 2_4:+9266816 None:intergenic
TGAATATAAACGGTTGAATT+TGG 0.154165 2_4:-9265817 Msa0313820:three_prime_UTR
ACCGACAACTCTTGACATTT+TGG 0.155414 2_4:+9266412 None:intergenic
TTTGTATCTCTTTGATTTAA+AGG 0.178789 2_4:+9266175 None:intergenic
ACAAAGCTTGCATAAGAATT+TGG 0.181591 2_4:-9266119 Msa0313820:CDS
TAACTTGTTGGTCACGTTAA+TGG 0.225258 2_4:-9266506 Msa0313820:CDS
TTCATCATCTTCTTCTATTT+GGG 0.225551 2_4:-9269078 Msa0313820:CDS
CGGAGGATTCACAGCGCTTT+CGG 0.245617 2_4:+9268991 None:intergenic
GGAGGATTCACAGCGCTTTC+GGG 0.279689 2_4:+9268992 None:intergenic
TTCATCACAAAGAGATAAAA+TGG 0.283330 2_4:-9266359 Msa0313820:CDS
GCAAGCCCTTTAGTTTCTTA+TGG 0.289989 2_4:-9266598 Msa0313820:CDS
TTCTGAAATTTCTTTGACTA+AGG 0.295883 2_4:+9266549 None:intergenic
CAAAGTTATAGCGTCGATTT+CGG 0.304348 2_4:-9265889 Msa0313820:three_prime_UTR
CTTTAATTCAACAACTTTGT+AGG 0.304965 2_4:-9265793 Msa0313820:three_prime_UTR
TGTAGGTGCTACACCAATTC+AGG 0.306517 2_4:-9265776 Msa0313820:three_prime_UTR
ACGGTGGACTGTTGCCATTT+TGG 0.315369 2_4:-9265937 Msa0313820:three_prime_UTR
AAGGAAAGTTCTAAGAGAAT+TGG 0.315481 2_4:+9266568 None:intergenic
GGCTCCTAAACATGATCTTT+TGG 0.319278 2_4:+9266651 None:intergenic
GGAGGAGGAGAGGGAGGAGG+TGG 0.327726 2_4:-9268871 Msa0313820:CDS
ATTCCAAATCTTGGTGTTTG+TGG 0.341246 2_4:+9266205 None:intergenic
TGTGCCGGTGTTGGTATCTA+TGG 0.344407 2_4:-9268706 Msa0313820:CDS
AATAACCAATGACTTGAGTT+TGG 0.347977 2_4:+9266057 None:intergenic
AACTTGTTGGTCACGTTAAT+GGG 0.351557 2_4:-9266505 Msa0313820:CDS
TCTACAAGGTCAATTGTCTT+AGG 0.352811 2_4:+9266808 None:intergenic
GGTCCACAAACACCAAGATT+TGG 0.367878 2_4:-9266208 Msa0313820:CDS
GATACCAAAAGATCATGTTT+AGG 0.369581 2_4:-9266655 Msa0313820:CDS
ACAGCGCTTTCGGGTTGCTT+TGG 0.370779 2_4:+9269001 None:intergenic
TAACTCTGCAAACTCACTTA+TGG 0.378473 2_4:+9266777 None:intergenic
TTAAGAATGTCTTTAAGAAA+TGG 0.384957 2_4:+9266694 None:intergenic
CAACTTCGAAGTCTTCAATA+AGG 0.402498 2_4:+9266013 None:intergenic
GTAAAGATGGGACCACATTC+TGG 0.405463 2_4:-9266229 Msa0313820:CDS
AGAAAATCTAATGAAGGAAA+AGG 0.408091 2_4:+9266718 None:intergenic
CGAAATCGACGCTATAACTT+TGG 0.417512 2_4:+9265890 None:intergenic
AGATGCTTCACTTTGGAAGC+TGG 0.420867 2_4:-9268815 Msa0313820:CDS
GGTATCTATGGTGGCTTCCC+AGG 0.423850 2_4:-9268694 Msa0313820:CDS
ACTCAAAGCGCAAATGCAAA+TGG 0.424290 2_4:-9266088 Msa0313820:CDS
TGCTACTTTAGGTAACTTGT+TGG 0.425049 2_4:-9266518 Msa0313820:CDS
GAAGCATAAGGAGGAGGAGA+GGG 0.427827 2_4:-9268880 Msa0313820:CDS
CTCAAAGCGCAAATGCAAAT+GGG 0.429397 2_4:-9266087 Msa0313820:CDS
CCATGAGTTCGACATGATTA+CGG 0.431320 2_4:-9265956 Msa0313820:three_prime_UTR
AGGAAAGTTCTAAGAGAATT+GGG 0.434479 2_4:+9266569 None:intergenic
GCATAAGGAGGAGGAGAGGG+AGG 0.434717 2_4:-9268877 Msa0313820:CDS
TAAGGAGGAGGAGAGGGAGG+AGG 0.439148 2_4:-9268874 Msa0313820:CDS
CATGAATATCTGAATATAAA+CGG 0.450941 2_4:-9265827 Msa0313820:three_prime_UTR
GTGGTTAGTCATCGGAGAGC+AGG 0.452074 2_4:-9268672 Msa0313820:CDS
TATGGAGAAGCATAAGGAGG+AGG 0.454646 2_4:-9268886 Msa0313820:CDS
GATGCTTCACTTTGGAAGCT+GGG 0.458178 2_4:-9268814 Msa0313820:CDS
ACCAAAATGTCAAGAGTTGT+CGG 0.458853 2_4:-9266413 Msa0313820:CDS
CTCTCCGATGACTAACCACC+TGG 0.459532 2_4:+9268676 None:intergenic
GGTGACCCCATTCCAAATCT+TGG 0.459651 2_4:+9266196 None:intergenic
AAAACAACTTCAAGAAGTAT+AGG 0.466913 2_4:+9266319 None:intergenic
GTCCTCCAAACTCAAGTCAT+TGG 0.475448 2_4:-9266062 Msa0313820:CDS
GAAAATCTAATGAAGGAAAA+GGG 0.479557 2_4:+9266719 None:intergenic
GATTGAAACAGTGCAAGTTA+TGG 0.479849 2_4:-9268904 Msa0313820:CDS
TCTTCAATAAGGCAAGCTCT+TGG 0.481042 2_4:+9266024 None:intergenic
AATCAAAGAGATACAAATGA+AGG 0.481857 2_4:-9266169 Msa0313820:CDS
GAAAGTTGATAACAGAACTT+GGG 0.485027 2_4:-9268731 Msa0313820:CDS
CATTGTTGAAAGGTTGTACA+AGG 0.492312 2_4:+9266630 None:intergenic
GGATGAAAGACAAGTAAAGA+TGG 0.494341 2_4:-9266242 Msa0313820:CDS
AGAAAGTTGATAACAGAACT+TGG 0.496648 2_4:-9268732 Msa0313820:CDS
CAAGACCATAAGAAACTAAA+GGG 0.497099 2_4:+9266593 None:intergenic
TTTGCAGCCACACAGAGAAT+CGG 0.497423 2_4:+9265857 None:intergenic
CAGGAACAAGGCTATGATAG+TGG 0.505796 2_4:-9266854 Msa0313820:CDS
AACCAATGACTTGAGTTTGG+AGG 0.512382 2_4:+9266060 None:intergenic
CAAACACCAAGATTTGGAAT+GGG 0.513089 2_4:-9266202 Msa0313820:CDS
CTTTGGAAGCTGGGAAGAGA+AGG 0.513641 2_4:-9268805 Msa0313820:CDS
GCAAGTTATGGAGAAGCATA+AGG 0.517239 2_4:-9268892 Msa0313820:CDS
GAGATAAAATGGCACAAGCT+AGG 0.518471 2_4:-9266348 Msa0313820:CDS
AATTCTTTACTCGTATCAAA+CGG 0.519898 2_4:-9269034 Msa0313820:CDS
AATGGAAGAAAATCTAATGA+AGG 0.520831 2_4:+9266712 None:intergenic
ACAGTGAAGATGCTTCACTT+TGG 0.523676 2_4:-9268822 Msa0313820:CDS
ACAAGACCATAAGAAACTAA+AGG 0.539120 2_4:+9266592 None:intergenic
ACAAACACCAAGATTTGGAA+TGG 0.539160 2_4:-9266203 Msa0313820:CDS
AACAGAACTTGGGGTTGTGC+CGG 0.544571 2_4:-9268721 Msa0313820:CDS
GAGCAATGAAACTATTGCAA+TGG 0.549239 2_4:-9266270 Msa0313820:CDS
CAAATAGAAGAAGATGATGA+AGG 0.552922 2_4:+9269080 None:intergenic
AGAAGCATAAGGAGGAGGAG+AGG 0.553180 2_4:-9268881 Msa0313820:CDS
ACGTTGATTTACCTAGCTGA+TGG 0.553971 2_4:-9268642 Msa0313820:CDS
GTATTCTTTCTTCAGGAACA+AGG 0.556956 2_4:-9266866 Msa0313820:intron
ACTTGGGGTTGTGCCGGTGT+TGG 0.560426 2_4:-9268715 Msa0313820:CDS
CCGTAATCATGTCGAACTCA+TGG 0.560647 2_4:+9265956 None:intergenic
GATGAAAGACAAGTAAAGAT+GGG 0.560923 2_4:-9266241 Msa0313820:CDS
TTCTTTGTTTAAATAATCGG+AGG 0.562901 2_4:+9268974 None:intergenic
AGCAATAGAGAACAACTCAA+TGG 0.577734 2_4:-9266476 Msa0313820:CDS
CTTCCCAGGTGGTTAGTCAT+CGG 0.577768 2_4:-9268680 Msa0313820:CDS
TGGAAGCTGGGAAGAGAAGG+AGG 0.583399 2_4:-9268802 Msa0313820:CDS
GGTGTTTGTGGACCAGAATG+TGG 0.586193 2_4:+9266217 None:intergenic
AAGGCTATGATAGTGGAGGC+AGG 0.586256 2_4:-9266847 Msa0313820:CDS
ATCAAAGAGATACAAATGAA+GGG 0.587935 2_4:-9266168 Msa0313820:CDS
ATGGTCAATTCAAACATACA+TGG 0.591645 2_4:+9265975 None:intergenic
ATCTATGGTGGCTTCCCAGG+TGG 0.600429 2_4:-9268691 Msa0313820:CDS
TGGTTAGTCATCGGAGAGCA+GGG 0.605588 2_4:-9268671 Msa0313820:CDS
AAGCAGAGCTGAACAGAACA+AGG 0.613079 2_4:-9268949 Msa0313820:CDS
ATCTCAGCCGATTCTCTGTG+TGG 0.614183 2_4:-9265864 Msa0313820:three_prime_UTR
TGATGAAGGAGTTGACATGC+TGG 0.618863 2_4:+9269094 None:intergenic
TTATGGTTGACTCATCTACA+AGG 0.635954 2_4:+9266794 None:intergenic
ACGTGTAAGTTCCATCAGCT+AGG 0.637555 2_4:+9268631 None:intergenic
TCAAAGCGCAAATGCAAATG+GGG 0.642603 2_4:-9266086 Msa0313820:CDS
GCCGGTGTTGGTATCTATGG+TGG 0.644634 2_4:-9268703 Msa0313820:CDS
AAAGTTGATAACAGAACTTG+GGG 0.647620 2_4:-9268730 Msa0313820:CDS
AAACACCAAGATTTGGAATG+GGG 0.656392 2_4:-9266201 Msa0313820:CDS
GAACAAGGCTATGATAGTGG+AGG 0.667903 2_4:-9266851 Msa0313820:CDS
AGTTATGGAGAAGCATAAGG+AGG 0.680310 2_4:-9268889 Msa0313820:CDS
GCCACCATAGATACCAACAC+CGG 0.692048 2_4:+9268702 None:intergenic
TGAGTTCGACATGATTACGG+TGG 0.717362 2_4:-9265953 Msa0313820:three_prime_UTR
TCTCCGATGACTAACCACCT+GGG 0.727974 2_4:+9268677 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCTTTGTTTAAATAAT+CGG + chr2_4:9265906-9265925 None:intergenic 10.0%
!! TTGTATAAAATATAATTAAG+TGG + chr2_4:9267158-9267177 None:intergenic 10.0%
!!! TTATATTTTATACAAGAAAT+TGG - chr2_4:9267163-9267182 Msa0313820:intron 10.0%
!! AATAAAATTAACATTTCAAA+TGG - chr2_4:9267402-9267421 Msa0313820:intron 10.0%
!! TTAATGCAATTTAATTAATT+TGG + chr2_4:9267510-9267529 None:intergenic 10.0%
!!! CTAAAAAACATTGTAATTTT+AGG + chr2_4:9266443-9266462 None:intergenic 15.0%
!! ATGTTATTCCAAAATTATTA+CGG - chr2_4:9267090-9267109 Msa0313820:intron 15.0%
!! ACATTAAATAAAAAACAGTT+TGG + chr2_4:9267326-9267345 None:intergenic 15.0%
!! GAAAAAAAAAATTAATGTGT+TGG + chr2_4:9267824-9267843 None:intergenic 15.0%
!! TTTAAAGAGTCTAAATTGTT+GGG - chr2_4:9266648-9266667 Msa0313820:CDS 20.0%
!!! GATATAAACCGTAATAATTT+TGG + chr2_4:9267101-9267120 None:intergenic 20.0%
!! CAAGATTTATTAATACAAAG+TGG - chr2_4:9267255-9267274 Msa0313820:intron 20.0%
!! TATTAATACAAAGTGGTTAT+TGG - chr2_4:9267262-9267281 Msa0313820:intron 20.0%
!! AAAATTAACATTTCAAATGG+AGG - chr2_4:9267405-9267424 Msa0313820:intron 20.0%
!!! TTAATTTGATGCCAAAATAT+TGG + chr2_4:9267561-9267580 None:intergenic 20.0%
!! ATATTAAATCACCAAAATGA+TGG - chr2_4:9267651-9267670 Msa0313820:intron 20.0%
!! TATTAAATCACCAAAATGAT+GGG - chr2_4:9267652-9267671 Msa0313820:intron 20.0%
!! TAATAAAATACAGTATAGGT+AGG - chr2_4:9267735-9267754 Msa0313820:intron 20.0%
!!! TTAAGAATGTCTTTAAGAAA+TGG + chr2_4:9268183-9268202 None:intergenic 20.0%
!!! AAAATCTCAACAAAATTTTG+TGG + chr2_4:9268426-9268445 None:intergenic 20.0%
!!! TTTGTATCTCTTTGATTTAA+AGG + chr2_4:9268702-9268721 None:intergenic 20.0%
!! CATGAATATCTGAATATAAA+CGG - chr2_4:9269047-9269066 Msa0313820:CDS 20.0%
!!! AATACTTTAAAAACCTGAAT+TGG + chr2_4:9269114-9269133 None:intergenic 20.0%
! TTCATCATCTTCTTCTATTT+GGG - chr2_4:9265796-9265815 Msa0313820:three_prime_UTR 25.0%
! AATTCTTTACTCGTATCAAA+CGG - chr2_4:9265840-9265859 Msa0313820:three_prime_UTR 25.0%
!! TTCTTTGTTTAAATAATCGG+AGG + chr2_4:9265903-9265922 None:intergenic 25.0%
! TAGAGACATTTCATATATTG+TGG + chr2_4:9266345-9266364 None:intergenic 25.0%
! ATGTGTTCTTAAGATGTTAT+TGG - chr2_4:9266604-9266623 Msa0313820:CDS 25.0%
! TGGTTAAAATGTTGAATTGA+TGG - chr2_4:9266624-9266643 Msa0313820:CDS 25.0%
! GGTTAAAATGTTGAATTGAT+GGG - chr2_4:9266625-9266644 Msa0313820:CDS 25.0%
! GTTTAAAGAGTCTAAATTGT+TGG - chr2_4:9266647-9266666 Msa0313820:CDS 25.0%
! CATCGTAGATGTTTATTTAA+TGG - chr2_4:9266698-9266717 Msa0313820:CDS 25.0%
! AATAAAAAACAGTTTGGTTG+TGG + chr2_4:9267320-9267339 None:intergenic 25.0%
! GAATTTGAAGAATTACACTT+TGG + chr2_4:9267364-9267383 None:intergenic 25.0%
!! ATGGAATTTAGCCAATATTT+TGG - chr2_4:9267547-9267566 Msa0313820:intron 25.0%
! TTTCTATGTGAAGTGTATTA+TGG + chr2_4:9267609-9267628 None:intergenic 25.0%
! CTGCTAATAAAATACAGTAT+AGG - chr2_4:9267731-9267750 Msa0313820:intron 25.0%
! GAAAATCTAATGAAGGAAAA+GGG + chr2_4:9268158-9268177 None:intergenic 25.0%
! AGAAAATCTAATGAAGGAAA+AGG + chr2_4:9268159-9268178 None:intergenic 25.0%
! AATGGAAGAAAATCTAATGA+AGG + chr2_4:9268165-9268184 None:intergenic 25.0%
!! CATTTTCTAACATTGTTGAA+AGG + chr2_4:9268257-9268276 None:intergenic 25.0%
!!! TTCTGAAATTTCTTTGACTA+AGG + chr2_4:9268328-9268347 None:intergenic 25.0%
!! ATTTTGTTGAGATTTTGTCA+TGG - chr2_4:9268430-9268449 Msa0313820:intron 25.0%
!! TTTTGTTGAGATTTTGTCAT+GGG - chr2_4:9268431-9268450 Msa0313820:intron 25.0%
! TTCATCACAAAGAGATAAAA+TGG - chr2_4:9268515-9268534 Msa0313820:intron 25.0%
!! AAAACAACTTCAAGAAGTAT+AGG + chr2_4:9268558-9268577 None:intergenic 25.0%
!!! ACTTCTTGAAGTTGTTTTAT+TGG - chr2_4:9268560-9268579 Msa0313820:intron 25.0%
!!! CTTCTTGAAGTTGTTTTATT+GGG - chr2_4:9268561-9268580 Msa0313820:intron 25.0%
!!! TTCTTGAAGTTGTTTTATTG+GGG - chr2_4:9268562-9268581 Msa0313820:intron 25.0%
! AATCAAAGAGATACAAATGA+AGG - chr2_4:9268705-9268724 Msa0313820:CDS 25.0%
! ATCAAAGAGATACAAATGAA+GGG - chr2_4:9268706-9268725 Msa0313820:CDS 25.0%
! GAAAAAATTGATGAAGAGTT+TGG - chr2_4:9268728-9268747 Msa0313820:CDS 25.0%
! TGAATATAAACGGTTGAATT+TGG - chr2_4:9269057-9269076 Msa0313820:CDS 25.0%
! CTTTAATTCAACAACTTTGT+AGG - chr2_4:9269081-9269100 Msa0313820:CDS 25.0%
CAAATAGAAGAAGATGATGA+AGG + chr2_4:9265797-9265816 None:intergenic 30.0%
CTTCATCATCTTCTTCTATT+TGG - chr2_4:9265795-9265814 Msa0313820:three_prime_UTR 30.0%
! CTGTTTCAATCTTTTCAACA+TGG + chr2_4:9265961-9265980 None:intergenic 30.0%
!! AGAAAGTTGATAACAGAACT+TGG - chr2_4:9266142-9266161 Msa0313820:CDS 30.0%
!! GAAAGTTGATAACAGAACTT+GGG - chr2_4:9266143-9266162 Msa0313820:CDS 30.0%
!! AAAGTTGATAACAGAACTTG+GGG - chr2_4:9266144-9266163 Msa0313820:CDS 30.0%
TCTACGATGAATATGAACAT+AGG + chr2_4:9266687-9266706 None:intergenic 30.0%
!!! TAACCACTGATTTTGACTTT+CGG - chr2_4:9267221-9267240 Msa0313820:intron 30.0%
! ATACAAAGTGGTTATTGGTT+TGG - chr2_4:9267267-9267286 Msa0313820:intron 30.0%
AATGACATCAAATCTGATGA+TGG - chr2_4:9267528-9267547 Msa0313820:intron 30.0%
ACTTCACATAGAAATTGAGT+TGG - chr2_4:9267615-9267634 Msa0313820:intron 30.0%
! GTATTAACTTGGTCAAAGTT+TGG + chr2_4:9267960-9267979 None:intergenic 30.0%
! AAGGAAAGCATGTATTAACT+TGG + chr2_4:9267971-9267990 None:intergenic 30.0%
GTCAATTGTCTTAGGTAATT+TGG + chr2_4:9268061-9268080 None:intergenic 30.0%
GATACCAAAAGATCATGTTT+AGG - chr2_4:9268219-9268238 Msa0313820:intron 30.0%
CAAGACCATAAGAAACTAAA+GGG + chr2_4:9268284-9268303 None:intergenic 30.0%
ACAAGACCATAAGAAACTAA+AGG + chr2_4:9268285-9268304 None:intergenic 30.0%
!! AGGAAAGTTCTAAGAGAATT+GGG + chr2_4:9268308-9268327 None:intergenic 30.0%
!! AAGGAAAGTTCTAAGAGAAT+TGG + chr2_4:9268309-9268328 None:intergenic 30.0%
GAAACTATTGCAATGGTTTA+AGG - chr2_4:9268611-9268630 Msa0313820:intron 30.0%
GATGAAAGACAAGTAAAGAT+GGG - chr2_4:9268633-9268652 Msa0313820:CDS 30.0%
ACAAAGCTTGCATAAGAATT+TGG - chr2_4:9268755-9268774 Msa0313820:CDS 30.0%
AATAACCAATGACTTGAGTT+TGG + chr2_4:9268820-9268839 None:intergenic 30.0%
ATGGTCAATTCAAACATACA+TGG + chr2_4:9268902-9268921 None:intergenic 30.0%
GATTGAAACAGTGCAAGTTA+TGG - chr2_4:9265970-9265989 Msa0313820:three_prime_UTR 35.0%
! CAAGAAATTGGTGATAGTTG+AGG - chr2_4:9267175-9267194 Msa0313820:intron 35.0%
!!! AACCACTGATTTTGACTTTC+GGG - chr2_4:9267222-9267241 Msa0313820:intron 35.0%
ACACACGTTAGGTATAAAGA+AGG + chr2_4:9267990-9268009 None:intergenic 35.0%
GAAGAAAGAATACACACGTT+AGG + chr2_4:9268001-9268020 None:intergenic 35.0%
AACGTGTGTATTCTTTCTTC+AGG - chr2_4:9268001-9268020 Msa0313820:intron 35.0%
GTATTCTTTCTTCAGGAACA+AGG - chr2_4:9268008-9268027 Msa0313820:intron 35.0%
TCTACAAGGTCAATTGTCTT+AGG + chr2_4:9268069-9268088 None:intergenic 35.0%
! TTATGGTTGACTCATCTACA+AGG + chr2_4:9268083-9268102 None:intergenic 35.0%
TAACTCTGCAAACTCACTTA+TGG + chr2_4:9268100-9268119 None:intergenic 35.0%
CATTGTTGAAAGGTTGTACA+AGG + chr2_4:9268247-9268266 None:intergenic 35.0%
!! GAAAAAGCTCTTGCTACTTT+AGG - chr2_4:9268345-9268364 Msa0313820:intron 35.0%
!! TGCTACTTTAGGTAACTTGT+TGG - chr2_4:9268356-9268375 Msa0313820:intron 35.0%
!! TAACTTGTTGGTCACGTTAA+TGG - chr2_4:9268368-9268387 Msa0313820:intron 35.0%
!! AACTTGTTGGTCACGTTAAT+GGG - chr2_4:9268369-9268388 Msa0313820:intron 35.0%
AGCAATAGAGAACAACTCAA+TGG - chr2_4:9268398-9268417 Msa0313820:intron 35.0%
ACCAAAATGTCAAGAGTTGT+CGG - chr2_4:9268461-9268480 Msa0313820:intron 35.0%
! ATTGCTCTCTTTTGAGCTAA+AGG + chr2_4:9268591-9268610 None:intergenic 35.0%
GAGCAATGAAACTATTGCAA+TGG - chr2_4:9268604-9268623 Msa0313820:intron 35.0%
GGATGAAAGACAAGTAAAGA+TGG - chr2_4:9268632-9268651 Msa0313820:intron 35.0%
!! ATTCCAAATCTTGGTGTTTG+TGG + chr2_4:9268672-9268691 None:intergenic 35.0%
ACAAACACCAAGATTTGGAA+TGG - chr2_4:9268671-9268690 Msa0313820:CDS 35.0%
! CAAACACCAAGATTTGGAAT+GGG - chr2_4:9268672-9268691 Msa0313820:CDS 35.0%
! AAACACCAAGATTTGGAATG+GGG - chr2_4:9268673-9268692 Msa0313820:CDS 35.0%
CAACTTCGAAGTCTTCAATA+AGG + chr2_4:9268864-9268883 None:intergenic 35.0%
! CACTGTATGTTTTGCCAAAA+TGG + chr2_4:9268954-9268973 None:intergenic 35.0%
!! CAAAGTTATAGCGTCGATTT+CGG - chr2_4:9268985-9269004 Msa0313820:CDS 35.0%
! GCAAGTTATGGAGAAGCATA+AGG - chr2_4:9265982-9266001 Msa0313820:three_prime_UTR 40.0%
! AGTTATGGAGAAGCATAAGG+AGG - chr2_4:9265985-9266004 Msa0313820:three_prime_UTR 40.0%
ACAGTGAAGATGCTTCACTT+TGG - chr2_4:9266052-9266071 Msa0313820:CDS 40.0%
!! ACGTTGATTTACCTAGCTGA+TGG - chr2_4:9266232-9266251 Msa0313820:CDS 40.0%
!!! ACCACTGATTTTGACTTTCG+GGG - chr2_4:9267223-9267242 Msa0313820:intron 40.0%
!!! TTTTGACTTTCGGGGGAATA+AGG - chr2_4:9267231-9267250 Msa0313820:intron 40.0%
!! CAAAGTGGTTATTGGTTTGG+CGG - chr2_4:9267270-9267289 Msa0313820:intron 40.0%
!! AAAGTGGTTATTGGTTTGGC+GGG - chr2_4:9267271-9267290 Msa0313820:intron 40.0%
TACACTTTGGTTGTGACTTG+TGG + chr2_4:9267351-9267370 None:intergenic 40.0%
!! ACCAAAATGATGGGCTACTA+GGG - chr2_4:9267661-9267680 Msa0313820:intron 40.0%
CAAGAGCTGAACAATGCTAT+AGG + chr2_4:9267891-9267910 None:intergenic 40.0%
! GGCTCCTAAACATGATCTTT+TGG + chr2_4:9268226-9268245 None:intergenic 40.0%
! GCAAGCCCTTTAGTTTCTTA+TGG - chr2_4:9268276-9268295 Msa0313820:intron 40.0%
! ACCGACAACTCTTGACATTT+TGG + chr2_4:9268465-9268484 None:intergenic 40.0%
GAGATAAAATGGCACAAGCT+AGG - chr2_4:9268526-9268545 Msa0313820:intron 40.0%
ACTCAAAGCGCAAATGCAAA+TGG - chr2_4:9268786-9268805 Msa0313820:CDS 40.0%
CTCAAAGCGCAAATGCAAAT+GGG - chr2_4:9268787-9268806 Msa0313820:CDS 40.0%
TCAAAGCGCAAATGCAAATG+GGG - chr2_4:9268788-9268807 Msa0313820:CDS 40.0%
AACCAATGACTTGAGTTTGG+AGG + chr2_4:9268817-9268836 None:intergenic 40.0%
TCTTCAATAAGGCAAGCTCT+TGG + chr2_4:9268853-9268872 None:intergenic 40.0%
CCATGAGTTCGACATGATTA+CGG - chr2_4:9268918-9268937 Msa0313820:CDS 40.0%
CGAAATCGACGCTATAACTT+TGG + chr2_4:9268987-9269006 None:intergenic 40.0%
TGATGAAGGAGTTGACATGC+TGG + chr2_4:9265783-9265802 None:intergenic 45.0%
AAGCAGAGCTGAACAGAACA+AGG - chr2_4:9265925-9265944 Msa0313820:three_prime_UTR 45.0%
! TATGGAGAAGCATAAGGAGG+AGG - chr2_4:9265988-9266007 Msa0313820:three_prime_UTR 45.0%
! AGATGCTTCACTTTGGAAGC+TGG - chr2_4:9266059-9266078 Msa0313820:CDS 45.0%
! GATGCTTCACTTTGGAAGCT+GGG - chr2_4:9266060-9266079 Msa0313820:CDS 45.0%
ACGTGTAAGTTCCATCAGCT+AGG + chr2_4:9266246-9266265 None:intergenic 45.0%
! TGGTGATAGTTGAGGCATCA+TGG - chr2_4:9267183-9267202 Msa0313820:intron 45.0%
! GGTGATAGTTGAGGCATCAT+GGG - chr2_4:9267184-9267203 Msa0313820:intron 45.0%
!!! CCACTGATTTTGACTTTCGG+GGG - chr2_4:9267224-9267243 Msa0313820:intron 45.0%
CAGTTTGGTTGTGGTTAGTG+TGG + chr2_4:9267311-9267330 None:intergenic 45.0%
AATGGAGGCAGAGAGAATCA+TGG - chr2_4:9267420-9267439 Msa0313820:intron 45.0%
TTCACGTTCTACTGCCTGTA+GGG + chr2_4:9267482-9267501 None:intergenic 45.0%
TTTCACGTTCTACTGCCTGT+AGG + chr2_4:9267483-9267502 None:intergenic 45.0%
!! CACCAAAATGATGGGCTACT+AGG - chr2_4:9267660-9267679 Msa0313820:intron 45.0%
!! CCAAAATGATGGGCTACTAG+GGG - chr2_4:9267662-9267681 Msa0313820:intron 45.0%
CAGGAACAAGGCTATGATAG+TGG - chr2_4:9268020-9268039 Msa0313820:intron 45.0%
!! GAACAAGGCTATGATAGTGG+AGG - chr2_4:9268023-9268042 Msa0313820:intron 45.0%
GTAAAGATGGGACCACATTC+TGG - chr2_4:9268645-9268664 Msa0313820:CDS 45.0%
GGTCCACAAACACCAAGATT+TGG - chr2_4:9268666-9268685 Msa0313820:CDS 45.0%
GTCCTCCAAACTCAAGTCAT+TGG - chr2_4:9268812-9268831 Msa0313820:CDS 45.0%
CCGTAATCATGTCGAACTCA+TGG + chr2_4:9268921-9268940 None:intergenic 45.0%
TGAGTTCGACATGATTACGG+TGG - chr2_4:9268921-9268940 Msa0313820:CDS 45.0%
TTTGCAGCCACACAGAGAAT+CGG + chr2_4:9269020-9269039 None:intergenic 45.0%
! TGTAGGTGCTACACCAATTC+AGG - chr2_4:9269098-9269117 Msa0313820:exon 45.0%
!!! AAATAATTTATTTTTTTGAA+AGG - chr2_4:9266971-9266990 Msa0313820:intron 5.0%
! AGAAGCATAAGGAGGAGGAG+AGG - chr2_4:9265993-9266012 Msa0313820:three_prime_UTR 50.0%
GAAGCATAAGGAGGAGGAGA+GGG - chr2_4:9265994-9266013 Msa0313820:three_prime_UTR 50.0%
! CTTTGGAAGCTGGGAAGAGA+AGG - chr2_4:9266069-9266088 Msa0313820:CDS 50.0%
AACAGAACTTGGGGTTGTGC+CGG - chr2_4:9266153-9266172 Msa0313820:CDS 50.0%
!! TGTGCCGGTGTTGGTATCTA+TGG - chr2_4:9266168-9266187 Msa0313820:CDS 50.0%
GCCACCATAGATACCAACAC+CGG + chr2_4:9266175-9266194 None:intergenic 50.0%
! CTTCCCAGGTGGTTAGTCAT+CGG - chr2_4:9266194-9266213 Msa0313820:CDS 50.0%
TCTCCGATGACTAACCACCT+GGG + chr2_4:9266200-9266219 None:intergenic 50.0%
! TGGTTAGTCATCGGAGAGCA+GGG - chr2_4:9266203-9266222 Msa0313820:CDS 50.0%
ATTGTGGCACGAGCTTGAAC+TGG + chr2_4:9266329-9266348 None:intergenic 50.0%
CCCCCGAAAGTCAAAATCAG+TGG + chr2_4:9267227-9267246 None:intergenic 50.0%
GAGGCAGAGAGAATCATGGT+GGG - chr2_4:9267424-9267443 Msa0313820:intron 50.0%
TCACGTTCTACTGCCTGTAG+GGG + chr2_4:9267481-9267500 None:intergenic 50.0%
! CCCCTAGTAGCCCATCATTT+TGG + chr2_4:9267665-9267684 None:intergenic 50.0%
!! AAGGCTATGATAGTGGAGGC+AGG - chr2_4:9268027-9268046 Msa0313820:intron 50.0%
GGTGTTTGTGGACCAGAATG+TGG + chr2_4:9268660-9268679 None:intergenic 50.0%
GGTGACCCCATTCCAAATCT+TGG + chr2_4:9268681-9268700 None:intergenic 50.0%
!!! ACGGTGGACTGTTGCCATTT+TGG - chr2_4:9268937-9268956 Msa0313820:CDS 50.0%
! ATCTCAGCCGATTCTCTGTG+TGG - chr2_4:9269010-9269029 Msa0313820:CDS 50.0%
!! ACAGCGCTTTCGGGTTGCTT+TGG + chr2_4:9265876-9265895 None:intergenic 55.0%
GGAGGATTCACAGCGCTTTC+GGG + chr2_4:9265885-9265904 None:intergenic 55.0%
CGGAGGATTCACAGCGCTTT+CGG + chr2_4:9265886-9265905 None:intergenic 55.0%
TGGAAGCTGGGAAGAGAAGG+AGG - chr2_4:9266072-9266091 Msa0313820:CDS 55.0%
!! GCCGGTGTTGGTATCTATGG+TGG - chr2_4:9266171-9266190 Msa0313820:CDS 55.0%
GGTATCTATGGTGGCTTCCC+AGG - chr2_4:9266180-9266199 Msa0313820:CDS 55.0%
ATCTATGGTGGCTTCCCAGG+TGG - chr2_4:9266183-9266202 Msa0313820:CDS 55.0%
CTCTCCGATGACTAACCACC+TGG + chr2_4:9266201-9266220 None:intergenic 55.0%
! GTGGTTAGTCATCGGAGAGC+AGG - chr2_4:9266202-9266221 Msa0313820:CDS 55.0%
GGAGGCAGAGAGAATCATGG+TGG - chr2_4:9267423-9267442 Msa0313820:intron 55.0%
GAGTTGAGTGTCTCCCCTAC+AGG - chr2_4:9267465-9267484 Msa0313820:intron 55.0%
GCATAAGGAGGAGGAGAGGG+AGG - chr2_4:9265997-9266016 Msa0313820:three_prime_UTR 60.0%
TAAGGAGGAGGAGAGGGAGG+AGG - chr2_4:9266000-9266019 Msa0313820:three_prime_UTR 60.0%
! ACTTGGGGTTGTGCCGGTGT+TGG - chr2_4:9266159-9266178 Msa0313820:CDS 60.0%
GGAGGAGGAGAGGGAGGAGG+TGG - chr2_4:9266003-9266022 Msa0313820:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 9265757 9269139 9265757 ID=Msa0313820;Name=Msa0313820
chr2_4 mRNA 9265757 9269139 9265757 ID=Msa0313820-mRNA-1;Parent=Msa0313820;Name=Msa0313820-mRNA-1;_AED=0.00;_eAED=0.00;_QI=28|1|1|1|1|1|2|270|441
chr2_4 exon 9265757 9266873 9265757 ID=Msa0313820-mRNA-1:exon:3567;Parent=Msa0313820-mRNA-1
chr2_4 exon 9268633 9269139 9268633 ID=Msa0313820-mRNA-1:exon:3566;Parent=Msa0313820-mRNA-1
chr2_4 five_prime_UTR 9269112 9269139 9269112 ID=Msa0313820-mRNA-1:five_prime_utr;Parent=Msa0313820-mRNA-1
chr2_4 CDS 9268633 9269111 9268633 ID=Msa0313820-mRNA-1:cds;Parent=Msa0313820-mRNA-1
chr2_4 CDS 9266027 9266873 9266027 ID=Msa0313820-mRNA-1:cds;Parent=Msa0313820-mRNA-1
chr2_4 three_prime_UTR 9265757 9266026 9265757 ID=Msa0313820-mRNA-1:three_prime_utr;Parent=Msa0313820-mRNA-1
Gene Sequence

>Msa0313820

ATGTCAACTCCTTCATCATCTTCTTCTATTTGGGTACTGTCTAACATAAAGCTTCAATTCTTTACTCGTATCAAACGGTTTCTTCAATCCAAAGCAACCCGAAAGCGCTGTGAATCCTCCGATTATTTAAACAAAGAAAAAAGCAGAGCTGAACAGAACAAGGTTGAACAGAACCATGTTGAAAAGATTGAAACAGTGCAAGTTATGGAGAAGCATAAGGAGGAGGAGAGGGAGGAGGTGGAAGAAACTGAAATTTTGTTGCAAAAGACAGTGAAGATGCTTCACTTTGGAAGCTGGGAAGAGAAGGAGGTAGCTGCAAAAGAGATTGAAAGTTTAGTTAAACAAGATGTTAAAGTAAGAAAGTTGATAACAGAACTTGGGGTTGTGCCGGTGTTGGTATCTATGGTGGCTTCCCAGGTGGTTAGTCATCGGAGAGCAGGGTTGACGACGTTGATTTACCTAGCTGATGGAACTTACACGAACAAGGCTATGATAGTGGAGGCAGGAATATTATCCAAATTACCTAAGACAATTGACCTTGTAGATGAGTCAACCATAAGTGAGTTTGCAGAGTTACTTTTATCATTATCATCACTTGCAAACACACAATACCCTTTTCCTTCATTAGATTTTCTTCCATTTCTTAAAGACATTCTTAAAAAAGACATGACTTTTGATACCAAAAGATCATGTTTAGGAGCCTTGTACAACCTTTCAACAATGTTAGAAAATGCAAGCCCTTTAGTTTCTTATGGTCTTGTCCCAATTCTCTTAGAACTTTCCTTAGTCAAAGAAATTTCAGAAAAAGCTCTTGCTACTTTAGGTAACTTGTTGGTCACGTTAATGGGTAAAAAAGCAATAGAGAACAACTCAATGGTGCCACAAAATTTTGTTGAGATTTTGTCATGGGAAGATAAACCAAAATGTCAAGAGTTGTCGGTTTATATTTTGATGATTTTAGCACATCAAAGTTCATCACAAAGAGATAAAATGGCACAAGCTAGGATTGTTCCTATACTTCTTGAAGTTGTTTTATTGGGGAGTCCTTTAGCTCAAAAGAGAGCAATGAAACTATTGCAATGGTTTAAGGATGAAAGACAAGTAAAGATGGGACCACATTCTGGTCCACAAACACCAAGATTTGGAATGGGGTCACCTTTAAATCAAAGAGATACAAATGAAGGGAAAAAATTGATGAAGAGTTTGGTGAAACAAAGCTTGCATAAGAATTTGGAAATAATAACTCAAAGCGCAAATGCAAATGGGGAGTCCTCCAAACTCAAGTCATTGGTTATTAGCACAAGTTCCAAGAGCTTGCCTTATTGA
Protein sequence

>Msa0313820

MSTPSSSSSIWVLSNIKLQFFTRIKRFLQSKATRKRCESSDYLNKEKSRAEQNKVEQNHVEKIETVQVMEKHKEEEREEVEETEILLQKTVKMLHFGSWEEKEVAAKEIESLVKQDVKVRKLITELGVVPVLVSMVASQVVSHRRAGLTTLIYLADGTYTNKAMIVEAGILSKLPKTIDLVDESTISEFAELLLSLSSLANTQYPFPSLDFLPFLKDILKKDMTFDTKRSCLGALYNLSTMLENASPLVSYGLVPILLELSLVKEISEKALATLGNLLVTLMGKKAIENNSMVPQNFVEILSWEDKPKCQELSVYILMILAHQSSSQRDKMAQARIVPILLEVVLLGSPLAQKRAMKLLQWFKDERQVKMGPHSGPQTPRFGMGSPLNQRDTNEGKKLMKSLVKQSLHKNLEIITQSANANGESSKLKSLVISTSSKSLPY*