AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0256710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256710 MtrunA17_Chr2g0309241 93.860 114 4 1 1 111 1 114 6.53e-69 202
Msa0256710 MtrunA17_Chr4g0012951 43.478 115 60 3 1 111 1 114 7.10e-24 88.2
Msa0256710 MtrunA17_Chr4g0013001 40.000 115 64 3 1 111 1 114 9.85e-21 80.1
Msa0256710 MtrunA17_Chr4g0012961 40.000 115 64 3 1 111 1 114 1.13e-20 80.1
Msa0256710 MtrunA17_Chr4g0012991 40.000 115 64 3 1 111 56 169 4.86e-20 80.1
Msa0256710 MtrunA17_Chr4g0012891 35.537 121 69 3 1 112 6 126 9.33e-19 75.5
Msa0256710 MtrunA17_Chr4g0013261 37.931 116 66 4 1 111 1 115 5.53e-18 73.2
Msa0256710 MtrunA17_Chr4g0013251 38.793 116 65 4 1 111 1 115 1.76e-17 72.0
Msa0256710 MtrunA17_Chr4g0013211 36.283 113 63 5 6 111 4 114 4.76e-16 68.2
Msa0256710 MtrunA17_Chr7g0244841 33.333 105 67 1 10 111 13 117 1.29e-15 67.4
Msa0256710 MtrunA17_Chr7g0244821 32.727 110 74 0 1 110 7 116 2.87e-15 66.6
Msa0256710 MtrunA17_Chr7g0244831 30.000 110 73 2 6 111 9 118 9.48e-15 65.1
Msa0256710 MtrunA17_Chr4g0012901 28.704 108 73 2 8 111 13 120 2.79e-14 63.9
Msa0256710 MtrunA17_Chr7g0233431 32.456 114 70 3 1 111 21 130 6.80e-14 63.2
Msa0256710 MtrunA17_Chr7g0244691 31.429 105 68 2 11 111 14 118 2.88e-13 61.2
Msa0256710 MtrunA17_Chr7g0244641 30.631 111 72 3 6 111 9 119 1.07e-12 59.7
Msa0256710 MtrunA17_Chr7g0244811 30.631 111 75 2 1 109 8 118 3.16e-12 58.5
Msa0256710 MtrunA17_Chr7g0244681 30.000 110 76 1 1 109 7 116 6.55e-12 57.8
Msa0256710 MtrunA17_Chr1g0165761 32.432 111 74 1 2 111 8 118 7.62e-12 57.4
Msa0256710 MtrunA17_Chr7g0244671 31.429 105 67 3 10 109 13 117 6.26e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256710 AT2G18370.1 53.211 109 48 1 6 111 7 115 6.43e-35 116
Msa0256710 AT5G59320.1 40.870 115 63 3 1 111 1 114 2.30e-21 82.0
Msa0256710 AT5G59310.1 35.965 114 67 3 1 111 1 111 2.59e-19 77.0
Msa0256710 AT3G51590.1 37.143 105 61 3 11 111 12 115 9.58e-17 70.5
Msa0256710 AT5G01870.1 39.806 103 54 4 15 111 15 115 3.09e-16 68.9
Msa0256710 AT3G08770.1 37.500 96 53 3 22 111 18 112 1.18e-14 65.1
Msa0256710 AT3G08770.2 38.710 93 50 3 22 108 18 109 2.09e-14 64.3
Msa0256710 AT2G38540.1 36.458 96 55 3 21 111 23 117 2.08e-13 62.0
Msa0256710 AT2G15050.3 31.481 108 65 4 3 101 2 109 3.58e-13 61.2
Msa0256710 AT2G15050.2 31.481 108 65 4 3 101 2 109 3.96e-13 61.2
Msa0256710 AT2G15050.1 31.481 108 65 4 3 101 2 109 4.39e-13 61.2
Msa0256710 AT3G51600.1 33.962 106 64 3 11 111 13 117 5.25e-13 60.8
Msa0256710 AT4G33355.2 34.951 103 61 4 11 109 16 116 5.42e-13 60.8
Msa0256710 AT4G33355.1 34.286 105 63 4 11 111 16 118 5.85e-13 60.8

Find 22 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTAAAGAAGATTTCA+TGG 0.285915 2_2:-51693015 None:intergenic
CAATTGGCTCAGGCTCTTGC+AGG 0.325545 2_2:+51693278 Msa0256710:CDS
GAAACCTTGTGTGAGCTATT+TGG 0.326436 2_2:+51693118 Msa0256710:CDS
TACTTGGAACAATCAGCATC+TGG 0.351088 2_2:-51693335 None:intergenic
CCACCAGCTGCATGTTGCTC+TGG 0.412892 2_2:+51693158 Msa0256710:CDS
AGCTATTTGGTCAGTGGAAG+TGG 0.469665 2_2:+51693131 Msa0256710:CDS
TATTAAAATAAACTCACAAT+TGG 0.472842 2_2:+51693262 Msa0256710:CDS
CTTTGTTGCTTCAAATTCAG+AGG 0.497380 2_2:+51693061 Msa0256710:CDS
AATAAACTCACAATTGGCTC+AGG 0.514463 2_2:+51693268 Msa0256710:CDS
ACTTGGAACAATCAGCATCT+GGG 0.519962 2_2:-51693334 None:intergenic
GCTCCAGAGCAACATGCAGC+TGG 0.542595 2_2:-51693161 None:intergenic
CAGGCTCTTGCAGGGAACTG+TGG 0.566184 2_2:+51693287 Msa0256710:CDS
AGCATCTGGGGAGATACTGA+TGG 0.572323 2_2:-51693321 None:intergenic
GCATCTGGGGAGATACTGAT+GGG 0.583176 2_2:-51693320 None:intergenic
AATTGGCTCAGGCTCTTGCA+GGG 0.601314 2_2:+51693279 Msa0256710:CDS
CTTGGAACAATCAGCATCTG+GGG 0.606095 2_2:-51693333 None:intergenic
CCAGAGCAACATGCAGCTGG+TGG 0.627457 2_2:-51693158 None:intergenic
ATCAGCTGTATCAACATCTG+AGG 0.627591 2_2:+51693196 Msa0256710:CDS
TGTGTGAGCTATTTGGTCAG+TGG 0.644010 2_2:+51693125 Msa0256710:CDS
CTGACCAAATAGCTCACACA+AGG 0.667347 2_2:-51693122 None:intergenic
ATCTGGGGAGATACTGATGG+GGG 0.675132 2_2:-51693318 None:intergenic
CATCTGGGGAGATACTGATG+GGG 0.712150 2_2:-51693319 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATAAATAAGTAAATAA+AGG - chr2_2:51693713-51693732 None:intergenic 10.0%
!! AAAGATAAATAAGTAAATAA+AGG - chr2_2:51693713-51693732 None:intergenic 10.0%
!! TATTAAAATAAACTCACAAT+TGG + chr2_2:51693262-51693281 Msa0256710:CDS 15.0%
!!! TTTAATGTATGATTTTTACT+TGG - chr2_2:51693354-51693373 None:intergenic 15.0%
!!! TGAAACAACTTTTTTTTTAT+AGG + chr2_2:51693500-51693519 Msa0256710:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG - chr2_2:51693607-51693626 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG - chr2_2:51693649-51693668 None:intergenic 15.0%
!! TATTAAAATAAACTCACAAT+TGG + chr2_2:51693262-51693281 Msa0256710:CDS 15.0%
!!! TTTAATGTATGATTTTTACT+TGG - chr2_2:51693354-51693373 None:intergenic 15.0%
!!! TGAAACAACTTTTTTTTTAT+AGG + chr2_2:51693500-51693519 Msa0256710:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG - chr2_2:51693607-51693626 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG - chr2_2:51693649-51693668 None:intergenic 15.0%
!!! TGAGTTTATTTTAATACTCT+TGG - chr2_2:51693258-51693277 None:intergenic 20.0%
!!! ACTTTTTTTTTATAGGAGAT+TGG + chr2_2:51693507-51693526 Msa0256710:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG + chr2_2:51693671-51693690 Msa0256710:intron 20.0%
!! GAAAGAAAAATTATTAACCT+TGG - chr2_2:51693691-51693710 None:intergenic 20.0%
!!! TGAGTTTATTTTAATACTCT+TGG - chr2_2:51693258-51693277 None:intergenic 20.0%
!!! ACTTTTTTTTTATAGGAGAT+TGG + chr2_2:51693507-51693526 Msa0256710:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG + chr2_2:51693671-51693690 Msa0256710:intron 20.0%
!! GAAAGAAAAATTATTAACCT+TGG - chr2_2:51693691-51693710 None:intergenic 20.0%
!!! TATTTTAATACTCTTGGAAG+TGG - chr2_2:51693252-51693271 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG + chr2_2:51693399-51693418 Msa0256710:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG + chr2_2:51693400-51693419 Msa0256710:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG + chr2_2:51693546-51693565 Msa0256710:intron 25.0%
! CAATTTGATATTGGAAAACT+TGG - chr2_2:51693598-51693617 None:intergenic 25.0%
!!! TATTTTAATACTCTTGGAAG+TGG - chr2_2:51693252-51693271 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG + chr2_2:51693399-51693418 Msa0256710:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG + chr2_2:51693400-51693419 Msa0256710:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG + chr2_2:51693546-51693565 Msa0256710:intron 25.0%
! CAATTTGATATTGGAAAACT+TGG - chr2_2:51693598-51693617 None:intergenic 25.0%
CTTCGAGACTAATCTTTAAT+CGG + chr2_2:51693474-51693493 Msa0256710:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG + chr2_2:51693545-51693564 Msa0256710:intron 30.0%
CTTCGAGACTAATCTTTAAT+CGG + chr2_2:51693474-51693493 Msa0256710:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG + chr2_2:51693545-51693564 Msa0256710:intron 30.0%
! CTTTGTTGCTTCAAATTCAG+AGG + chr2_2:51693061-51693080 Msa0256710:CDS 35.0%
TTCATGCAGTGATGTGATTA+AGG + chr2_2:51693091-51693110 Msa0256710:intron 35.0%
! AATAAACTCACAATTGGCTC+AGG + chr2_2:51693268-51693287 Msa0256710:CDS 35.0%
AGATTGGACTGCTTATCTAT+TGG + chr2_2:51693523-51693542 Msa0256710:intron 35.0%
GATTGGACTGCTTATCTATT+GGG + chr2_2:51693524-51693543 Msa0256710:intron 35.0%
!! ATTGCAGGATTGGTTAATTG+AGG + chr2_2:51693747-51693766 Msa0256710:intron 35.0%
! CTTTGTTGCTTCAAATTCAG+AGG + chr2_2:51693061-51693080 Msa0256710:CDS 35.0%
TTCATGCAGTGATGTGATTA+AGG + chr2_2:51693091-51693110 Msa0256710:intron 35.0%
! AATAAACTCACAATTGGCTC+AGG + chr2_2:51693268-51693287 Msa0256710:CDS 35.0%
AGATTGGACTGCTTATCTAT+TGG + chr2_2:51693523-51693542 Msa0256710:intron 35.0%
GATTGGACTGCTTATCTATT+GGG + chr2_2:51693524-51693543 Msa0256710:intron 35.0%
!! ATTGCAGGATTGGTTAATTG+AGG + chr2_2:51693747-51693766 Msa0256710:intron 35.0%
GAAACCTTGTGTGAGCTATT+TGG + chr2_2:51693118-51693137 Msa0256710:CDS 40.0%
ATCAGCTGTATCAACATCTG+AGG + chr2_2:51693196-51693215 Msa0256710:CDS 40.0%
ACTTGGAACAATCAGCATCT+GGG - chr2_2:51693337-51693356 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG - chr2_2:51693338-51693357 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG - chr2_2:51693411-51693430 None:intergenic 40.0%
GTTAATTGAGGCTTCGAAGT+GGG + chr2_2:51693759-51693778 Msa0256710:intron 40.0%
GAAACCTTGTGTGAGCTATT+TGG + chr2_2:51693118-51693137 Msa0256710:CDS 40.0%
ATCAGCTGTATCAACATCTG+AGG + chr2_2:51693196-51693215 Msa0256710:CDS 40.0%
ACTTGGAACAATCAGCATCT+GGG - chr2_2:51693337-51693356 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG - chr2_2:51693338-51693357 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG - chr2_2:51693411-51693430 None:intergenic 40.0%
GTTAATTGAGGCTTCGAAGT+GGG + chr2_2:51693759-51693778 Msa0256710:intron 40.0%
CTGACCAAATAGCTCACACA+AGG - chr2_2:51693125-51693144 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG + chr2_2:51693125-51693144 Msa0256710:CDS 45.0%
AGCTATTTGGTCAGTGGAAG+TGG + chr2_2:51693131-51693150 Msa0256710:CDS 45.0%
CTTGGAACAATCAGCATCTG+GGG - chr2_2:51693336-51693355 None:intergenic 45.0%
!! TGTAGCGATTTTGATACCCG+AGG - chr2_2:51693434-51693453 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG + chr2_2:51693732-51693751 Msa0256710:intron 45.0%
GGTTAATTGAGGCTTCGAAG+TGG + chr2_2:51693758-51693777 Msa0256710:intron 45.0%
CTGACCAAATAGCTCACACA+AGG - chr2_2:51693125-51693144 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG + chr2_2:51693125-51693144 Msa0256710:CDS 45.0%
AGCTATTTGGTCAGTGGAAG+TGG + chr2_2:51693131-51693150 Msa0256710:CDS 45.0%
CTTGGAACAATCAGCATCTG+GGG - chr2_2:51693336-51693355 None:intergenic 45.0%
!! TGTAGCGATTTTGATACCCG+AGG - chr2_2:51693434-51693453 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG + chr2_2:51693732-51693751 Msa0256710:intron 45.0%
GGTTAATTGAGGCTTCGAAG+TGG + chr2_2:51693758-51693777 Msa0256710:intron 45.0%
! AATTGGCTCAGGCTCTTGCA+GGG + chr2_2:51693279-51693298 Msa0256710:CDS 50.0%
ATCTGGGGAGATACTGATGG+GGG - chr2_2:51693321-51693340 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG - chr2_2:51693322-51693341 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG - chr2_2:51693323-51693342 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG - chr2_2:51693324-51693343 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG + chr2_2:51693414-51693433 Msa0256710:intron 50.0%
!! AGCGATTTTGATACCCGAGG+TGG - chr2_2:51693431-51693450 None:intergenic 50.0%
TGCTGCGTCTATTGCAGGAT+TGG + chr2_2:51693737-51693756 Msa0256710:intron 50.0%
TGAGGCTTCGAAGTGGGAAT+TGG + chr2_2:51693765-51693784 Msa0256710:intron 50.0%
GCTTCGAAGTGGGAATTGGA+GGG + chr2_2:51693769-51693788 Msa0256710:intron 50.0%
! AATTGGCTCAGGCTCTTGCA+GGG + chr2_2:51693279-51693298 Msa0256710:CDS 50.0%
ATCTGGGGAGATACTGATGG+GGG - chr2_2:51693321-51693340 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG - chr2_2:51693322-51693341 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG - chr2_2:51693323-51693342 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG - chr2_2:51693324-51693343 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG + chr2_2:51693414-51693433 Msa0256710:intron 50.0%
!! AGCGATTTTGATACCCGAGG+TGG - chr2_2:51693431-51693450 None:intergenic 50.0%
TGCTGCGTCTATTGCAGGAT+TGG + chr2_2:51693737-51693756 Msa0256710:intron 50.0%
TGAGGCTTCGAAGTGGGAAT+TGG + chr2_2:51693765-51693784 Msa0256710:intron 50.0%
GCTTCGAAGTGGGAATTGGA+GGG + chr2_2:51693769-51693788 Msa0256710:intron 50.0%
! CAATTGGCTCAGGCTCTTGC+AGG + chr2_2:51693278-51693297 Msa0256710:CDS 55.0%
GAATAGGGTGTAGCCACCTC+GGG + chr2_2:51693415-51693434 Msa0256710:intron 55.0%
GGCTTCGAAGTGGGAATTGG+AGG + chr2_2:51693768-51693787 Msa0256710:intron 55.0%
! CAATTGGCTCAGGCTCTTGC+AGG + chr2_2:51693278-51693297 Msa0256710:CDS 55.0%
GAATAGGGTGTAGCCACCTC+GGG + chr2_2:51693415-51693434 Msa0256710:intron 55.0%
GGCTTCGAAGTGGGAATTGG+AGG + chr2_2:51693768-51693787 Msa0256710:intron 55.0%
CCAGAGCAACATGCAGCTGG+TGG - chr2_2:51693161-51693180 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG + chr2_2:51693158-51693177 Msa0256710:CDS 60.0%
GCTCCAGAGCAACATGCAGC+TGG - chr2_2:51693164-51693183 None:intergenic 60.0%
CAGGCTCTTGCAGGGAACTG+TGG + chr2_2:51693287-51693306 Msa0256710:CDS 60.0%
CCAGAGCAACATGCAGCTGG+TGG - chr2_2:51693161-51693180 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG + chr2_2:51693158-51693177 Msa0256710:CDS 60.0%
GCTCCAGAGCAACATGCAGC+TGG - chr2_2:51693164-51693183 None:intergenic 60.0%
CAGGCTCTTGCAGGGAACTG+TGG + chr2_2:51693287-51693306 Msa0256710:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 51693017 51693898 51693017 ID=Msa0256710;Name=Msa0256710
chr2_2 mRNA 51693017 51693898 51693017 ID=Msa0256710-mRNA-1;Parent=Msa0256710;Name=Msa0256710-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|0.66|0|0|3|0|112
chr2_2 exon 51693017 51693103 51693017 ID=Msa0256710-mRNA-1:exon:15597;Parent=Msa0256710-mRNA-1
chr2_2 exon 51693113 51693354 51693113 ID=Msa0256710-mRNA-1:exon:15598;Parent=Msa0256710-mRNA-1
chr2_2 exon 51693892 51693898 51693892 ID=Msa0256710-mRNA-1:exon:15599;Parent=Msa0256710-mRNA-1
chr2_2 CDS 51693017 51693103 51693017 ID=Msa0256710-mRNA-1:cds;Parent=Msa0256710-mRNA-1
chr2_2 CDS 51693113 51693354 51693113 ID=Msa0256710-mRNA-1:cds;Parent=Msa0256710-mRNA-1
chr2_2 CDS 51693892 51693898 51693892 ID=Msa0256710-mRNA-1:cds;Parent=Msa0256710-mRNA-1
Gene Sequence

>Msa0256710

ATGAAATCTTCTTTAATTGCATTTTCTGCTGTTGTGCTTCTCATCTTTGTTGCTTCAAATTCAGAGGCTGCAATTTCATGCAGTGATGACTTGAAACCTTGTGTGAGCTATTTGGTCAGTGGAAGTGGACAACCACCAGCTGCATGTTGCTCTGGAGCTAAAGCTCTAGCATCAGCTGTATCAACATCTGAGGATAAAAAAGCTGCTTGCAACTGCATCAAATCCACTTCCAAGAGTATTAAAATAAACTCACAATTGGCTCAGGCTCTTGCAGGGAACTGTGGAATTAACACCCCCATCAGTATCTCCCCAGATGCTGATTGTTCCAACATGTAT

Protein sequence

>Msa0256710

MKSSLIAFSAVVLLIFVASNSEAAISCSDDLKPCVSYLVSGSGQPPAACCSGAKALASAVSTSEDKKAACNCIKSTSKSIKINSQLAQALAGNCGINTPISISPDADCSNMY