AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0289960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0289960 MtrunA17_Chr2g0309241 93.860 114 4 1 1 111 1 114 6.53e-69 202
Msa0289960 MtrunA17_Chr4g0012951 43.478 115 60 3 1 111 1 114 7.10e-24 88.2
Msa0289960 MtrunA17_Chr4g0013001 40.000 115 64 3 1 111 1 114 9.85e-21 80.1
Msa0289960 MtrunA17_Chr4g0012961 40.000 115 64 3 1 111 1 114 1.13e-20 80.1
Msa0289960 MtrunA17_Chr4g0012991 40.000 115 64 3 1 111 56 169 4.86e-20 80.1
Msa0289960 MtrunA17_Chr4g0012891 35.537 121 69 3 1 112 6 126 9.33e-19 75.5
Msa0289960 MtrunA17_Chr4g0013261 37.931 116 66 4 1 111 1 115 5.53e-18 73.2
Msa0289960 MtrunA17_Chr4g0013251 38.793 116 65 4 1 111 1 115 1.76e-17 72.0
Msa0289960 MtrunA17_Chr4g0013211 36.283 113 63 5 6 111 4 114 4.76e-16 68.2
Msa0289960 MtrunA17_Chr7g0244841 33.333 105 67 1 10 111 13 117 1.29e-15 67.4
Msa0289960 MtrunA17_Chr7g0244821 32.727 110 74 0 1 110 7 116 2.87e-15 66.6
Msa0289960 MtrunA17_Chr7g0244831 30.000 110 73 2 6 111 9 118 9.48e-15 65.1
Msa0289960 MtrunA17_Chr4g0012901 28.704 108 73 2 8 111 13 120 2.79e-14 63.9
Msa0289960 MtrunA17_Chr7g0233431 32.456 114 70 3 1 111 21 130 6.80e-14 63.2
Msa0289960 MtrunA17_Chr7g0244691 31.429 105 68 2 11 111 14 118 2.88e-13 61.2
Msa0289960 MtrunA17_Chr7g0244641 30.631 111 72 3 6 111 9 119 1.07e-12 59.7
Msa0289960 MtrunA17_Chr7g0244811 30.631 111 75 2 1 109 8 118 3.16e-12 58.5
Msa0289960 MtrunA17_Chr7g0244681 30.000 110 76 1 1 109 7 116 6.55e-12 57.8
Msa0289960 MtrunA17_Chr1g0165761 32.432 111 74 1 2 111 8 118 7.62e-12 57.4
Msa0289960 MtrunA17_Chr7g0244671 31.429 105 67 3 10 109 13 117 6.26e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0289960 AT2G18370.1 53.211 109 48 1 6 111 7 115 6.43e-35 116
Msa0289960 AT5G59320.1 40.870 115 63 3 1 111 1 114 2.30e-21 82.0
Msa0289960 AT5G59310.1 35.965 114 67 3 1 111 1 111 2.59e-19 77.0
Msa0289960 AT3G51590.1 37.143 105 61 3 11 111 12 115 9.58e-17 70.5
Msa0289960 AT5G01870.1 39.806 103 54 4 15 111 15 115 3.09e-16 68.9
Msa0289960 AT3G08770.1 37.500 96 53 3 22 111 18 112 1.18e-14 65.1
Msa0289960 AT3G08770.2 38.710 93 50 3 22 108 18 109 2.09e-14 64.3
Msa0289960 AT2G38540.1 36.458 96 55 3 21 111 23 117 2.08e-13 62.0
Msa0289960 AT2G15050.3 31.481 108 65 4 3 101 2 109 3.58e-13 61.2
Msa0289960 AT2G15050.2 31.481 108 65 4 3 101 2 109 3.96e-13 61.2
Msa0289960 AT2G15050.1 31.481 108 65 4 3 101 2 109 4.39e-13 61.2
Msa0289960 AT3G51600.1 33.962 106 64 3 11 111 13 117 5.25e-13 60.8
Msa0289960 AT4G33355.2 34.951 103 61 4 11 109 16 116 5.42e-13 60.8
Msa0289960 AT4G33355.1 34.286 105 63 4 11 111 16 118 5.85e-13 60.8

Find 22 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTAAAGAAGATTTCA+TGG 0.285915 2_3:+36550794 None:intergenic
CAATTGGCTCAGGCTCTTGC+AGG 0.325545 2_3:-36550531 Msa0289960:CDS
GAAACCTTGTGTGAGCTATT+TGG 0.326436 2_3:-36550691 Msa0289960:CDS
TACTTGGAACAATCAGCATC+TGG 0.351088 2_3:+36550474 None:intergenic
CCACCAGCTGCATGTTGCTC+TGG 0.412892 2_3:-36550651 Msa0289960:CDS
AGCTATTTGGTCAGTGGAAG+TGG 0.469665 2_3:-36550678 Msa0289960:CDS
TATTAAAATCAACTCACAAT+TGG 0.472842 2_3:-36550547 Msa0289960:CDS
CTTTGTTGCTTCAAATTCAG+AGG 0.497380 2_3:-36550748 Msa0289960:CDS
AATCAACTCACAATTGGCTC+AGG 0.514463 2_3:-36550541 Msa0289960:CDS
ACTTGGAACAATCAGCATCT+GGG 0.519962 2_3:+36550475 None:intergenic
GCTCCAGAGCAACATGCAGC+TGG 0.542595 2_3:+36550648 None:intergenic
CAGGCTCTTGCAGGGAACTG+TGG 0.566184 2_3:-36550522 Msa0289960:CDS
AGCATCTGGGGAGATACTGA+TGG 0.572323 2_3:+36550488 None:intergenic
GCATCTGGGGAGATACTGAT+GGG 0.583176 2_3:+36550489 None:intergenic
AATTGGCTCAGGCTCTTGCA+GGG 0.601314 2_3:-36550530 Msa0289960:CDS
CTTGGAACAATCAGCATCTG+GGG 0.606095 2_3:+36550476 None:intergenic
CCAGAGCAACATGCAGCTGG+TGG 0.627457 2_3:+36550651 None:intergenic
ATCAGCTGTATCAACATCTG+AGG 0.627591 2_3:-36550613 Msa0289960:CDS
TGTGTGAGCTATTTGGTCAG+TGG 0.644010 2_3:-36550684 Msa0289960:CDS
CTGACCAAATAGCTCACACA+AGG 0.667347 2_3:+36550687 None:intergenic
ATCTGGGGAGATACTGATGG+GGG 0.675132 2_3:+36550491 None:intergenic
CATCTGGGGAGATACTGATG+GGG 0.712150 2_3:+36550490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATAAATAAGTAAATAA+AGG + chr2_3:36550667-36550686 None:intergenic 10.0%
!! AAAGATAAATAAGTAAATAA+AGG + chr2_3:36550667-36550686 None:intergenic 10.0%
!!! TTTAATGTATGATTTTTACT+TGG + chr2_3:36550309-36550328 None:intergenic 15.0%
!!! ATGAAACAACTTTTTTTTAT+AGG - chr2_3:36550454-36550473 Msa0289960:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG + chr2_3:36550561-36550580 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG + chr2_3:36550603-36550622 None:intergenic 15.0%
!!! TTTAATGTATGATTTTTACT+TGG + chr2_3:36550309-36550328 None:intergenic 15.0%
!!! ATGAAACAACTTTTTTTTAT+AGG - chr2_3:36550454-36550473 Msa0289960:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG + chr2_3:36550561-36550580 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG + chr2_3:36550603-36550622 None:intergenic 15.0%
!! TATTAAAATCAACTCACAAT+TGG - chr2_3:36550217-36550236 Msa0289960:intron 20.0%
!!! AACTTTTTTTTATAGGAGAT+TGG - chr2_3:36550461-36550480 Msa0289960:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG - chr2_3:36550625-36550644 Msa0289960:CDS 20.0%
!! GAAAGAAAAATTATTAACCT+TGG + chr2_3:36550645-36550664 None:intergenic 20.0%
!! TATTAAAATCAACTCACAAT+TGG - chr2_3:36550217-36550236 Msa0289960:intron 20.0%
!!! AACTTTTTTTTATAGGAGAT+TGG - chr2_3:36550461-36550480 Msa0289960:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG - chr2_3:36550625-36550644 Msa0289960:CDS 20.0%
!! GAAAGAAAAATTATTAACCT+TGG + chr2_3:36550645-36550664 None:intergenic 20.0%
!!! TGAGTTGATTTTAATACTCT+TGG + chr2_3:36550213-36550232 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG - chr2_3:36550354-36550373 Msa0289960:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG - chr2_3:36550355-36550374 Msa0289960:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG - chr2_3:36550500-36550519 Msa0289960:CDS 25.0%
! CAATTTGATATTGGAAAACT+TGG + chr2_3:36550552-36550571 None:intergenic 25.0%
!!! TGAGTTGATTTTAATACTCT+TGG + chr2_3:36550213-36550232 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG - chr2_3:36550354-36550373 Msa0289960:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG - chr2_3:36550355-36550374 Msa0289960:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG - chr2_3:36550500-36550519 Msa0289960:CDS 25.0%
! CAATTTGATATTGGAAAACT+TGG + chr2_3:36550552-36550571 None:intergenic 25.0%
!! GATTTTAATACTCTTGGAAG+TGG + chr2_3:36550207-36550226 None:intergenic 30.0%
CTTCGAGACTAATCTTTAAT+CGG - chr2_3:36550429-36550448 Msa0289960:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG - chr2_3:36550499-36550518 Msa0289960:CDS 30.0%
!! GATTTTAATACTCTTGGAAG+TGG + chr2_3:36550207-36550226 None:intergenic 30.0%
CTTCGAGACTAATCTTTAAT+CGG - chr2_3:36550429-36550448 Msa0289960:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG - chr2_3:36550499-36550518 Msa0289960:CDS 30.0%
! CTTTGTTGCTTCAAATTCAG+AGG - chr2_3:36550016-36550035 Msa0289960:intron 35.0%
TTCATGCAGTGATGTGATTA+AGG - chr2_3:36550046-36550065 Msa0289960:intron 35.0%
AGATTGGACTGCTTATCTAT+TGG - chr2_3:36550477-36550496 Msa0289960:CDS 35.0%
GATTGGACTGCTTATCTATT+GGG - chr2_3:36550478-36550497 Msa0289960:CDS 35.0%
GTTAATTGAGACTTCGAAGT+GGG - chr2_3:36550713-36550732 Msa0289960:intron 35.0%
! CTTTGTTGCTTCAAATTCAG+AGG - chr2_3:36550016-36550035 Msa0289960:intron 35.0%
TTCATGCAGTGATGTGATTA+AGG - chr2_3:36550046-36550065 Msa0289960:intron 35.0%
AGATTGGACTGCTTATCTAT+TGG - chr2_3:36550477-36550496 Msa0289960:CDS 35.0%
GATTGGACTGCTTATCTATT+GGG - chr2_3:36550478-36550497 Msa0289960:CDS 35.0%
GTTAATTGAGACTTCGAAGT+GGG - chr2_3:36550713-36550732 Msa0289960:intron 35.0%
GAAACCTTGTGTGAGCTATT+TGG - chr2_3:36550073-36550092 Msa0289960:intron 40.0%
ATCAGCTGTATCAACATCTG+AGG - chr2_3:36550151-36550170 Msa0289960:intron 40.0%
! AATCAACTCACAATTGGCTC+AGG - chr2_3:36550223-36550242 Msa0289960:intron 40.0%
ACTTGGAACAATCAGCATCT+GGG + chr2_3:36550292-36550311 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG + chr2_3:36550293-36550312 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG + chr2_3:36550366-36550385 None:intergenic 40.0%
GGTTAATTGAGACTTCGAAG+TGG - chr2_3:36550712-36550731 Msa0289960:intron 40.0%
GAAACCTTGTGTGAGCTATT+TGG - chr2_3:36550073-36550092 Msa0289960:intron 40.0%
ATCAGCTGTATCAACATCTG+AGG - chr2_3:36550151-36550170 Msa0289960:intron 40.0%
! AATCAACTCACAATTGGCTC+AGG - chr2_3:36550223-36550242 Msa0289960:intron 40.0%
ACTTGGAACAATCAGCATCT+GGG + chr2_3:36550292-36550311 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG + chr2_3:36550293-36550312 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG + chr2_3:36550366-36550385 None:intergenic 40.0%
GGTTAATTGAGACTTCGAAG+TGG - chr2_3:36550712-36550731 Msa0289960:intron 40.0%
CTGACCAAATAGCTCACACA+AGG + chr2_3:36550080-36550099 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG - chr2_3:36550080-36550099 Msa0289960:intron 45.0%
AGCTATTTGGTCAGTGGAAG+TGG - chr2_3:36550086-36550105 Msa0289960:intron 45.0%
CTTGGAACAATCAGCATCTG+GGG + chr2_3:36550291-36550310 None:intergenic 45.0%
!! TGTAGCGATTTTGATACCCG+AGG + chr2_3:36550389-36550408 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG - chr2_3:36550686-36550705 Msa0289960:CDS 45.0%
TGAGACTTCGAAGTGGGAAT+TGG - chr2_3:36550719-36550738 Msa0289960:intron 45.0%
ACTTCGAAGTGGGAATTGGA+GGG - chr2_3:36550723-36550742 Msa0289960:intron 45.0%
CTGACCAAATAGCTCACACA+AGG + chr2_3:36550080-36550099 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG - chr2_3:36550080-36550099 Msa0289960:intron 45.0%
AGCTATTTGGTCAGTGGAAG+TGG - chr2_3:36550086-36550105 Msa0289960:intron 45.0%
CTTGGAACAATCAGCATCTG+GGG + chr2_3:36550291-36550310 None:intergenic 45.0%
!! TGTAGCGATTTTGATACCCG+AGG + chr2_3:36550389-36550408 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG - chr2_3:36550686-36550705 Msa0289960:CDS 45.0%
TGAGACTTCGAAGTGGGAAT+TGG - chr2_3:36550719-36550738 Msa0289960:intron 45.0%
ACTTCGAAGTGGGAATTGGA+GGG - chr2_3:36550723-36550742 Msa0289960:intron 45.0%
! AATTGGCTCAGGCTCTTGCA+GGG - chr2_3:36550234-36550253 Msa0289960:intron 50.0%
ATCTGGGGAGATACTGATGG+GGG + chr2_3:36550276-36550295 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG + chr2_3:36550277-36550296 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG + chr2_3:36550278-36550297 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG + chr2_3:36550279-36550298 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG - chr2_3:36550369-36550388 Msa0289960:intron 50.0%
!! AGCGATTTTGATACCCGAGG+TGG + chr2_3:36550386-36550405 None:intergenic 50.0%
TGCTGCGTCTATTGCAGGAT+TGG - chr2_3:36550691-36550710 Msa0289960:CDS 50.0%
GACTTCGAAGTGGGAATTGG+AGG - chr2_3:36550722-36550741 Msa0289960:intron 50.0%
! AATTGGCTCAGGCTCTTGCA+GGG - chr2_3:36550234-36550253 Msa0289960:intron 50.0%
ATCTGGGGAGATACTGATGG+GGG + chr2_3:36550276-36550295 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG + chr2_3:36550277-36550296 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG + chr2_3:36550278-36550297 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG + chr2_3:36550279-36550298 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG - chr2_3:36550369-36550388 Msa0289960:intron 50.0%
!! AGCGATTTTGATACCCGAGG+TGG + chr2_3:36550386-36550405 None:intergenic 50.0%
TGCTGCGTCTATTGCAGGAT+TGG - chr2_3:36550691-36550710 Msa0289960:CDS 50.0%
GACTTCGAAGTGGGAATTGG+AGG - chr2_3:36550722-36550741 Msa0289960:intron 50.0%
! CAATTGGCTCAGGCTCTTGC+AGG - chr2_3:36550233-36550252 Msa0289960:intron 55.0%
GAATAGGGTGTAGCCACCTC+GGG - chr2_3:36550370-36550389 Msa0289960:intron 55.0%
! CAATTGGCTCAGGCTCTTGC+AGG - chr2_3:36550233-36550252 Msa0289960:intron 55.0%
GAATAGGGTGTAGCCACCTC+GGG - chr2_3:36550370-36550389 Msa0289960:intron 55.0%
CCAGAGCAACATGCAGCTGG+TGG + chr2_3:36550116-36550135 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG - chr2_3:36550113-36550132 Msa0289960:intron 60.0%
GCTCCAGAGCAACATGCAGC+TGG + chr2_3:36550119-36550138 None:intergenic 60.0%
CAGGCTCTTGCAGGGAACTG+TGG - chr2_3:36550242-36550261 Msa0289960:intron 60.0%
CCAGAGCAACATGCAGCTGG+TGG + chr2_3:36550116-36550135 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG - chr2_3:36550113-36550132 Msa0289960:intron 60.0%
GCTCCAGAGCAACATGCAGC+TGG + chr2_3:36550119-36550138 None:intergenic 60.0%
CAGGCTCTTGCAGGGAACTG+TGG - chr2_3:36550242-36550261 Msa0289960:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 36549972 36550814 36549972 ID=Msa0289960;Name=Msa0289960
chr2_3 mRNA 36549972 36550814 36549972 ID=Msa0289960-mRNA-1;Parent=Msa0289960;Name=Msa0289960-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|0.66|0|0|3|0|112
chr2_3 exon 36550728 36550814 36550728 ID=Msa0289960-mRNA-1:exon:11975;Parent=Msa0289960-mRNA-1
chr2_3 exon 36550477 36550718 36550477 ID=Msa0289960-mRNA-1:exon:11974;Parent=Msa0289960-mRNA-1
chr2_3 exon 36549972 36549978 36549972 ID=Msa0289960-mRNA-1:exon:11973;Parent=Msa0289960-mRNA-1
chr2_3 CDS 36550728 36550814 36550728 ID=Msa0289960-mRNA-1:cds;Parent=Msa0289960-mRNA-1
chr2_3 CDS 36550477 36550718 36550477 ID=Msa0289960-mRNA-1:cds;Parent=Msa0289960-mRNA-1
chr2_3 CDS 36549972 36549978 36549972 ID=Msa0289960-mRNA-1:cds;Parent=Msa0289960-mRNA-1
Gene Sequence

>Msa0289960

ATGAAATCTTCTTTAATTGCATTTTCTGCTGTTGTGCTTCTCATCTTTGTTGCTTCAAATTCAGAGGCTGCAATTTCATGCAGTGATGACTTGAAACCTTGTGTGAGCTATTTGGTCAGTGGAAGTGGACAACCACCAGCTGCATGTTGCTCTGGAGCTAAAGCTCTAGCATCAGCTGTATCAACATCTGAGGATAAAAAAGCTGCTTGCAACTGCATCAAATCCACTTCCAAGAGTATTAAAATCAACTCACAATTGGCTCAGGCTCTTGCAGGGAACTGTGGAATTAACACCCCCATCAGTATCTCCCCAGATGCTGATTGTTCCAACATGTAT

Protein sequence

>Msa0289960

MKSSLIAFSAVVLLIFVASNSEAAISCSDDLKPCVSYLVSGSGQPPAACCSGAKALASAVSTSEDKKAACNCIKSTSKSIKINSQLAQALAGNCGINTPISISPDADCSNMY