AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0327760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0327760 MtrunA17_Chr2g0309241 93.860 114 4 1 1 111 1 114 5.66e-69 202
Msa0327760 MtrunA17_Chr4g0012951 44.348 115 59 3 1 111 1 114 1.51e-24 90.1
Msa0327760 MtrunA17_Chr4g0013001 40.870 115 63 3 1 111 1 114 2.62e-21 81.6
Msa0327760 MtrunA17_Chr4g0012961 40.870 115 63 3 1 111 1 114 2.98e-21 81.6
Msa0327760 MtrunA17_Chr4g0012991 40.870 115 63 3 1 111 56 169 1.24e-20 81.6
Msa0327760 MtrunA17_Chr4g0012891 36.364 121 68 4 1 112 6 126 8.37e-19 75.5
Msa0327760 MtrunA17_Chr4g0013261 37.383 107 62 3 9 111 10 115 3.32e-18 73.9
Msa0327760 MtrunA17_Chr4g0013251 40.000 110 60 4 6 111 8 115 1.07e-17 72.4
Msa0327760 MtrunA17_Chr4g0013211 36.283 113 63 5 6 111 4 114 4.81e-16 68.2
Msa0327760 MtrunA17_Chr7g0244821 33.636 110 73 0 1 110 7 116 7.29e-16 68.2
Msa0327760 MtrunA17_Chr7g0244831 30.000 110 73 2 6 111 9 118 4.04e-15 65.9
Msa0327760 MtrunA17_Chr7g0244841 32.381 105 68 1 10 111 13 117 4.17e-15 65.9
Msa0327760 MtrunA17_Chr7g0233431 32.456 114 70 3 1 111 21 130 2.56e-14 64.3
Msa0327760 MtrunA17_Chr4g0012901 28.704 108 73 2 8 111 13 120 3.46e-14 63.5
Msa0327760 MtrunA17_Chr7g0244641 30.631 111 72 3 6 111 9 119 5.49e-13 60.5
Msa0327760 MtrunA17_Chr7g0244811 31.532 111 74 2 1 109 8 118 6.05e-13 60.5
Msa0327760 MtrunA17_Chr7g0244691 30.476 105 69 2 11 111 14 118 9.30e-13 60.1
Msa0327760 MtrunA17_Chr7g0244681 30.909 110 75 1 1 109 7 116 1.51e-12 59.3
Msa0327760 MtrunA17_Chr1g0165761 33.333 111 73 1 2 111 8 118 1.59e-12 59.3
Msa0327760 MtrunA17_Chr4g0012931 29.060 117 77 3 1 111 1 117 1.59e-11 57.0
Msa0327760 MtrunA17_Chr7g0244721 31.858 113 75 2 1 111 7 119 8.29e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0327760 AT2G18370.1 52.294 109 49 1 6 111 7 115 1.17e-34 115
Msa0327760 AT5G59320.1 41.739 115 62 3 1 111 1 114 1.31e-21 82.8
Msa0327760 AT5G59310.1 35.965 114 67 3 1 111 1 111 2.83e-19 76.6
Msa0327760 AT5G01870.1 38.318 107 59 3 11 111 10 115 8.90e-17 70.5
Msa0327760 AT3G51590.1 37.143 105 61 3 11 111 12 115 1.43e-16 70.1
Msa0327760 AT3G08770.1 37.500 96 53 3 22 111 18 112 1.20e-14 65.1
Msa0327760 AT3G08770.2 38.710 93 50 3 22 108 18 109 2.14e-14 64.3
Msa0327760 AT2G38540.1 33.621 116 69 4 1 111 5 117 8.89e-14 62.8
Msa0327760 AT2G15050.2 32.407 108 64 4 3 101 2 109 1.15e-13 62.4
Msa0327760 AT2G15050.3 32.407 108 64 4 3 101 2 109 1.32e-13 62.4
Msa0327760 AT2G15050.1 32.407 108 64 4 3 101 2 109 1.60e-13 62.4
Msa0327760 AT3G51600.1 32.479 117 70 4 3 111 2 117 7.33e-13 60.5
Msa0327760 AT4G33355.2 34.951 103 61 4 11 109 16 116 8.07e-13 60.5
Msa0327760 AT4G33355.1 32.727 110 68 4 6 111 11 118 9.09e-13 60.1

Find 22 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTAAAGAAGATTTCA+TGG 0.308768 2_4:+39702927 None:intergenic
GAAACCTTGTGTGAGCTATT+TGG 0.326436 2_4:-39702824 Msa0327760:CDS
CAATTGGCTCAAGCTCTTGC+AGG 0.347675 2_4:-39702664 Msa0327760:CDS
TACTTGGAACAATCAGCATC+TGG 0.351088 2_4:+39702607 None:intergenic
CCACCAGCTGCATGTTGCTC+TGG 0.412892 2_4:-39702784 Msa0327760:CDS
AATTGCAGCCTCTGAATTTG+AGG 0.443900 2_4:+39702873 None:intergenic
AGCTATTTGGTCAGTGGAAG+TGG 0.469665 2_4:-39702811 Msa0327760:CDS
TATTAAAATCAACTCACAAT+TGG 0.472842 2_4:-39702680 Msa0327760:CDS
ACTTGGAACAATCAGCATCT+GGG 0.519962 2_4:+39702608 None:intergenic
CTTTGTTGCCTCAAATTCAG+AGG 0.521553 2_4:-39702881 Msa0327760:CDS
GCTCCAGAGCAACATGCAGC+TGG 0.542595 2_4:+39702781 None:intergenic
CAAGCTCTTGCAGGGAACTG+TGG 0.564691 2_4:-39702655 Msa0327760:CDS
AGCATCTGGGGAGATACTGA+TGG 0.572323 2_4:+39702621 None:intergenic
AATTGGCTCAAGCTCTTGCA+GGG 0.579413 2_4:-39702663 Msa0327760:CDS
GCATCTGGGGAGATACTGAT+GGG 0.583176 2_4:+39702622 None:intergenic
CTTGGAACAATCAGCATCTG+GGG 0.606095 2_4:+39702609 None:intergenic
CCAGAGCAACATGCAGCTGG+TGG 0.627457 2_4:+39702784 None:intergenic
ATCAGCTGTATCAACATCTG+AGG 0.627591 2_4:-39702746 Msa0327760:CDS
TGTGTGAGCTATTTGGTCAG+TGG 0.644010 2_4:-39702817 Msa0327760:CDS
CTGACCAAATAGCTCACACA+AGG 0.667347 2_4:+39702820 None:intergenic
ATCTGGGGAGATACTGATGG+GGG 0.675132 2_4:+39702624 None:intergenic
CATCTGGGGAGATACTGATG+GGG 0.712150 2_4:+39702623 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATAAATAAGTAAATAA+AGG + chr2_4:39702778-39702797 None:intergenic 10.0%
!! AAAGATAAATAAGTAAATAA+AGG + chr2_4:39702778-39702797 None:intergenic 10.0%
!!! TTTAATGTATGATTTTTACT+TGG + chr2_4:39702420-39702439 None:intergenic 15.0%
!!! ATGAAACAACTTTTTTTTAT+AGG - chr2_4:39702565-39702584 Msa0327760:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG + chr2_4:39702672-39702691 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG + chr2_4:39702714-39702733 None:intergenic 15.0%
!!! TTTAATGTATGATTTTTACT+TGG + chr2_4:39702420-39702439 None:intergenic 15.0%
!!! ATGAAACAACTTTTTTTTAT+AGG - chr2_4:39702565-39702584 Msa0327760:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG + chr2_4:39702672-39702691 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG + chr2_4:39702714-39702733 None:intergenic 15.0%
!! TATTAAAATCAACTCACAAT+TGG - chr2_4:39702328-39702347 Msa0327760:intron 20.0%
!!! AACTTTTTTTTATAGGAGAT+TGG - chr2_4:39702572-39702591 Msa0327760:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG - chr2_4:39702736-39702755 Msa0327760:CDS 20.0%
!! GAAAGAAAAATTATTAACCT+TGG + chr2_4:39702756-39702775 None:intergenic 20.0%
!! TATTAAAATCAACTCACAAT+TGG - chr2_4:39702328-39702347 Msa0327760:intron 20.0%
!!! AACTTTTTTTTATAGGAGAT+TGG - chr2_4:39702572-39702591 Msa0327760:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG - chr2_4:39702736-39702755 Msa0327760:CDS 20.0%
!! GAAAGAAAAATTATTAACCT+TGG + chr2_4:39702756-39702775 None:intergenic 20.0%
!!! TGAGTTGATTTTAATACTCT+TGG + chr2_4:39702324-39702343 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG - chr2_4:39702465-39702484 Msa0327760:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG - chr2_4:39702466-39702485 Msa0327760:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG - chr2_4:39702611-39702630 Msa0327760:CDS 25.0%
! CAATTTGATATTGGAAAACT+TGG + chr2_4:39702663-39702682 None:intergenic 25.0%
!!! TGAGTTGATTTTAATACTCT+TGG + chr2_4:39702324-39702343 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG - chr2_4:39702465-39702484 Msa0327760:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG - chr2_4:39702466-39702485 Msa0327760:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG - chr2_4:39702611-39702630 Msa0327760:CDS 25.0%
! CAATTTGATATTGGAAAACT+TGG + chr2_4:39702663-39702682 None:intergenic 25.0%
!! GATTTTAATACTCTTGGAAG+TGG + chr2_4:39702318-39702337 None:intergenic 30.0%
CTTCGAGACTAATCTTTAAT+CGG - chr2_4:39702540-39702559 Msa0327760:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG - chr2_4:39702610-39702629 Msa0327760:CDS 30.0%
!! GATTTTAATACTCTTGGAAG+TGG + chr2_4:39702318-39702337 None:intergenic 30.0%
CTTCGAGACTAATCTTTAAT+CGG - chr2_4:39702540-39702559 Msa0327760:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG - chr2_4:39702610-39702629 Msa0327760:CDS 30.0%
TTCATGCAGTGATGTGATTA+AGG - chr2_4:39702157-39702176 Msa0327760:intron 35.0%
AGATTGGACTGCTTATCTAT+TGG - chr2_4:39702588-39702607 Msa0327760:intron 35.0%
GATTGGACTGCTTATCTATT+GGG - chr2_4:39702589-39702608 Msa0327760:intron 35.0%
!! ATTGCAGGATTGGTTAATTG+AGG - chr2_4:39702812-39702831 Msa0327760:CDS 35.0%
TTCATGCAGTGATGTGATTA+AGG - chr2_4:39702157-39702176 Msa0327760:intron 35.0%
AGATTGGACTGCTTATCTAT+TGG - chr2_4:39702588-39702607 Msa0327760:intron 35.0%
GATTGGACTGCTTATCTATT+GGG - chr2_4:39702589-39702608 Msa0327760:intron 35.0%
!! ATTGCAGGATTGGTTAATTG+AGG - chr2_4:39702812-39702831 Msa0327760:CDS 35.0%
CTTTGTTGCCTCAAATTCAG+AGG - chr2_4:39702127-39702146 Msa0327760:intron 40.0%
AATTGCAGCCTCTGAATTTG+AGG + chr2_4:39702138-39702157 None:intergenic 40.0%
GAAACCTTGTGTGAGCTATT+TGG - chr2_4:39702184-39702203 Msa0327760:intron 40.0%
ATCAGCTGTATCAACATCTG+AGG - chr2_4:39702262-39702281 Msa0327760:intron 40.0%
ACTTGGAACAATCAGCATCT+GGG + chr2_4:39702403-39702422 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG + chr2_4:39702404-39702423 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG + chr2_4:39702477-39702496 None:intergenic 40.0%
!! TGTAGCGATTTTGATATCCG+AGG + chr2_4:39702500-39702519 None:intergenic 40.0%
GTTAATTGAGGCTTCGAAGT+GGG - chr2_4:39702824-39702843 Msa0327760:CDS 40.0%
CTTTGTTGCCTCAAATTCAG+AGG - chr2_4:39702127-39702146 Msa0327760:intron 40.0%
AATTGCAGCCTCTGAATTTG+AGG + chr2_4:39702138-39702157 None:intergenic 40.0%
GAAACCTTGTGTGAGCTATT+TGG - chr2_4:39702184-39702203 Msa0327760:intron 40.0%
ATCAGCTGTATCAACATCTG+AGG - chr2_4:39702262-39702281 Msa0327760:intron 40.0%
ACTTGGAACAATCAGCATCT+GGG + chr2_4:39702403-39702422 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG + chr2_4:39702404-39702423 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG + chr2_4:39702477-39702496 None:intergenic 40.0%
!! TGTAGCGATTTTGATATCCG+AGG + chr2_4:39702500-39702519 None:intergenic 40.0%
GTTAATTGAGGCTTCGAAGT+GGG - chr2_4:39702824-39702843 Msa0327760:CDS 40.0%
CTGACCAAATAGCTCACACA+AGG + chr2_4:39702191-39702210 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG - chr2_4:39702191-39702210 Msa0327760:intron 45.0%
AGCTATTTGGTCAGTGGAAG+TGG - chr2_4:39702197-39702216 Msa0327760:intron 45.0%
! AATTGGCTCAAGCTCTTGCA+GGG - chr2_4:39702345-39702364 Msa0327760:intron 45.0%
CTTGGAACAATCAGCATCTG+GGG + chr2_4:39702402-39702421 None:intergenic 45.0%
!! AGCGATTTTGATATCCGAGG+TGG + chr2_4:39702497-39702516 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG - chr2_4:39702797-39702816 Msa0327760:CDS 45.0%
GGTTAATTGAGGCTTCGAAG+TGG - chr2_4:39702823-39702842 Msa0327760:CDS 45.0%
CTGACCAAATAGCTCACACA+AGG + chr2_4:39702191-39702210 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG - chr2_4:39702191-39702210 Msa0327760:intron 45.0%
AGCTATTTGGTCAGTGGAAG+TGG - chr2_4:39702197-39702216 Msa0327760:intron 45.0%
! AATTGGCTCAAGCTCTTGCA+GGG - chr2_4:39702345-39702364 Msa0327760:intron 45.0%
CTTGGAACAATCAGCATCTG+GGG + chr2_4:39702402-39702421 None:intergenic 45.0%
!! AGCGATTTTGATATCCGAGG+TGG + chr2_4:39702497-39702516 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG - chr2_4:39702797-39702816 Msa0327760:CDS 45.0%
GGTTAATTGAGGCTTCGAAG+TGG - chr2_4:39702823-39702842 Msa0327760:CDS 45.0%
! CAATTGGCTCAAGCTCTTGC+AGG - chr2_4:39702344-39702363 Msa0327760:intron 50.0%
ATCTGGGGAGATACTGATGG+GGG + chr2_4:39702387-39702406 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG + chr2_4:39702388-39702407 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG + chr2_4:39702389-39702408 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG + chr2_4:39702390-39702409 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG - chr2_4:39702480-39702499 Msa0327760:intron 50.0%
TGCTGCGTCTATTGCAGGAT+TGG - chr2_4:39702802-39702821 Msa0327760:CDS 50.0%
TGAGGCTTCGAAGTGGGAAT+TGG - chr2_4:39702830-39702849 Msa0327760:CDS 50.0%
GCTTCGAAGTGGGAATTGGA+GGG - chr2_4:39702834-39702853 Msa0327760:intron 50.0%
! CAATTGGCTCAAGCTCTTGC+AGG - chr2_4:39702344-39702363 Msa0327760:intron 50.0%
ATCTGGGGAGATACTGATGG+GGG + chr2_4:39702387-39702406 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG + chr2_4:39702388-39702407 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG + chr2_4:39702389-39702408 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG + chr2_4:39702390-39702409 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG - chr2_4:39702480-39702499 Msa0327760:intron 50.0%
TGCTGCGTCTATTGCAGGAT+TGG - chr2_4:39702802-39702821 Msa0327760:CDS 50.0%
TGAGGCTTCGAAGTGGGAAT+TGG - chr2_4:39702830-39702849 Msa0327760:CDS 50.0%
GCTTCGAAGTGGGAATTGGA+GGG - chr2_4:39702834-39702853 Msa0327760:intron 50.0%
CAAGCTCTTGCAGGGAACTG+TGG - chr2_4:39702353-39702372 Msa0327760:intron 55.0%
GGCTTCGAAGTGGGAATTGG+AGG - chr2_4:39702833-39702852 Msa0327760:intron 55.0%
CAAGCTCTTGCAGGGAACTG+TGG - chr2_4:39702353-39702372 Msa0327760:intron 55.0%
GGCTTCGAAGTGGGAATTGG+AGG - chr2_4:39702833-39702852 Msa0327760:intron 55.0%
CCAGAGCAACATGCAGCTGG+TGG + chr2_4:39702227-39702246 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG - chr2_4:39702224-39702243 Msa0327760:intron 60.0%
GCTCCAGAGCAACATGCAGC+TGG + chr2_4:39702230-39702249 None:intergenic 60.0%
CCAGAGCAACATGCAGCTGG+TGG + chr2_4:39702227-39702246 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG - chr2_4:39702224-39702243 Msa0327760:intron 60.0%
GCTCCAGAGCAACATGCAGC+TGG + chr2_4:39702230-39702249 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 39702083 39702947 39702083 ID=Msa0327760;Name=Msa0327760
chr2_4 mRNA 39702083 39702947 39702083 ID=Msa0327760-mRNA-1;Parent=Msa0327760;Name=Msa0327760-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|0.66|0|0|3|0|112
chr2_4 exon 39702861 39702947 39702861 ID=Msa0327760-mRNA-1:exon:12036;Parent=Msa0327760-mRNA-1
chr2_4 exon 39702610 39702851 39702610 ID=Msa0327760-mRNA-1:exon:12035;Parent=Msa0327760-mRNA-1
chr2_4 exon 39702083 39702089 39702083 ID=Msa0327760-mRNA-1:exon:12034;Parent=Msa0327760-mRNA-1
chr2_4 CDS 39702861 39702947 39702861 ID=Msa0327760-mRNA-1:cds;Parent=Msa0327760-mRNA-1
chr2_4 CDS 39702610 39702851 39702610 ID=Msa0327760-mRNA-1:cds;Parent=Msa0327760-mRNA-1
chr2_4 CDS 39702083 39702089 39702083 ID=Msa0327760-mRNA-1:cds;Parent=Msa0327760-mRNA-1
Gene Sequence

>Msa0327760

ATGAAATCTTCTTTAATTGCATTCGCTGCTCTTGTGCTTCTCATCTTTGTTGCCTCAAATTCAGAGGCTGCAATTTCATGCAGTGATGACTTGAAACCTTGTGTGAGCTATTTGGTCAGTGGAAGTGGACAACCACCAGCTGCATGTTGCTCTGGAGCTAAAGCTCTAGCATCAGCTGTATCAACATCTGAGGATAAAAAAGCTGCTTGCAACTGCATCAAATCCACTTCCAAGAGTATTAAAATCAACTCACAATTGGCTCAAGCTCTTGCAGGGAACTGTGGAATTAACACCCCCATCAGTATCTCCCCAGATGCTGATTGTTCCAACATGTAT

Protein sequence

>Msa0327760

MKSSLIAFAALVLLIFVASNSEAAISCSDDLKPCVSYLVSGSGQPPAACCSGAKALASAVSTSEDKKAACNCIKSTSKSIKINSQLAQALAGNCGINTPISISPDADCSNMY