AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0210610


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0210610 MtrunA17_Chr2g0309241 94.783 115 3 1 1 112 1 115 1.06e-70 206
Msa0210610 MtrunA17_Chr4g0012951 44.348 115 59 3 1 111 1 114 2.62e-24 89.4
Msa0210610 MtrunA17_Chr4g0013001 40.870 115 63 3 1 111 1 114 4.10e-21 81.3
Msa0210610 MtrunA17_Chr4g0012961 40.870 115 63 3 1 111 1 114 4.62e-21 81.3
Msa0210610 MtrunA17_Chr4g0012991 40.870 115 63 3 1 111 56 169 2.70e-20 80.5
Msa0210610 MtrunA17_Chr4g0012891 35.833 120 68 3 1 111 6 125 8.21e-19 75.5
Msa0210610 MtrunA17_Chr4g0013261 38.793 116 65 4 1 111 1 115 1.94e-18 74.3
Msa0210610 MtrunA17_Chr4g0013251 45.238 84 44 2 29 111 33 115 8.19e-18 72.8
Msa0210610 MtrunA17_Chr7g0244841 35.238 105 65 1 10 111 13 117 6.87e-17 70.5
Msa0210610 MtrunA17_Chr4g0013211 37.168 113 62 5 6 111 4 114 1.45e-16 69.7
Msa0210610 MtrunA17_Chr7g0244821 33.636 110 73 0 1 110 7 116 6.01e-16 68.2
Msa0210610 MtrunA17_Chr4g0012901 30.556 108 71 2 8 111 13 120 1.22e-15 67.4
Msa0210610 MtrunA17_Chr7g0244831 30.000 110 73 2 6 111 9 118 5.36e-15 65.9
Msa0210610 MtrunA17_Chr7g0233431 33.333 114 69 3 1 111 21 130 1.91e-14 64.7
Msa0210610 MtrunA17_Chr7g0244691 31.429 105 68 2 11 111 14 118 1.51e-13 62.0
Msa0210610 MtrunA17_Chr7g0244811 30.702 114 77 2 1 112 8 121 2.07e-13 61.6
Msa0210610 MtrunA17_Chr7g0244641 30.631 111 72 3 6 111 9 119 6.52e-13 60.5
Msa0210610 MtrunA17_Chr1g0165761 33.036 112 74 1 2 112 8 119 6.58e-13 60.5
Msa0210610 MtrunA17_Chr7g0244681 30.631 111 76 1 1 110 7 117 1.25e-12 59.7
Msa0210610 MtrunA17_Chr4g0012931 29.915 117 76 3 1 111 1 117 3.85e-12 58.5
Msa0210610 MtrunA17_Chr7g0244671 32.075 106 67 3 10 110 13 118 1.37e-11 57.4
Msa0210610 MtrunA17_Chr7g0244721 31.858 113 75 2 1 111 7 119 3.00e-11 55.8
Msa0210610 MtrunA17_Chr7g0244791 32.075 106 68 2 10 111 18 123 5.27e-11 55.5
Msa0210610 MtrunA17_Chr3g0107481 27.523 109 72 4 8 111 7 113 5.47e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0210610 AT2G18370.1 53.636 110 48 1 6 112 7 116 4.68e-36 119
Msa0210610 AT5G59320.1 40.870 115 63 3 1 111 1 114 6.62e-21 80.9
Msa0210610 AT5G59310.1 36.842 114 66 3 1 111 1 111 1.22e-19 77.8
Msa0210610 AT3G51590.1 38.095 105 60 3 11 111 12 115 3.82e-17 71.6
Msa0210610 AT5G01870.1 40.777 103 53 4 15 111 15 115 1.78e-16 69.7
Msa0210610 AT3G08770.1 38.542 96 52 3 22 111 18 112 6.20e-15 65.9
Msa0210610 AT3G08770.2 38.710 93 50 3 22 108 18 109 2.09e-14 64.3
Msa0210610 AT2G38540.1 36.458 96 55 3 21 111 23 117 1.13e-13 62.8
Msa0210610 AT4G33355.2 34.615 104 62 4 11 110 16 117 1.79e-13 62.0
Msa0210610 AT4G33355.1 35.238 105 62 4 11 111 16 118 2.02e-13 62.0
Msa0210610 AT3G51600.1 33.962 106 64 3 11 111 13 117 2.53e-13 61.6
Msa0210610 AT2G15050.2 31.481 108 65 4 3 101 2 109 4.17e-13 61.2
Msa0210610 AT2G15050.3 31.481 108 65 4 3 101 2 109 4.63e-13 61.2
Msa0210610 AT2G15050.1 31.481 108 65 4 3 101 2 109 5.87e-13 60.8

Find 28 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTAAAGAAGATTTCA+TGG 0.285915 2_1:-50836139 None:intergenic
CAATTGGCTCAGGCTCTTGC+AGG 0.325545 2_1:+50836402 Msa0210610:CDS
GAAACCTTGTGTGAGCTATT+TGG 0.326436 2_1:+50836242 Msa0210610:CDS
TACTTGGAACAATCAGCATC+TGG 0.351088 2_1:-50836459 None:intergenic
TGAGGCTTCGAAGTGGGAAT+TGG 0.374090 2_1:+50836889 Msa0210610:three_prime_UTR
CCACCAGCTGCATGTTGCTC+TGG 0.412892 2_1:+50836282 Msa0210610:CDS
AGCTATTTGGTCAGTGGAAG+TGG 0.469665 2_1:+50836255 Msa0210610:CDS
TATTAAAATCAACTCACAAT+TGG 0.472842 2_1:+50836386 Msa0210610:CDS
GGCTTCGAAGTGGGAATTGG+AGG 0.493556 2_1:+50836892 Msa0210610:three_prime_UTR
ATTGCAGGATTGGTTAATTG+AGG 0.494673 2_1:+50836871 Msa0210610:intron
CTTTGTTGCTTCAAATTCAG+AGG 0.497380 2_1:+50836185 Msa0210610:CDS
AATCAACTCACAATTGGCTC+AGG 0.514463 2_1:+50836392 Msa0210610:CDS
ACTTGGAACAATCAGCATCT+GGG 0.519962 2_1:-50836458 None:intergenic
GCTCCAGAGCAACATGCAGC+TGG 0.542595 2_1:-50836285 None:intergenic
CAGGCTCTTGCAGGGAACTG+TGG 0.566184 2_1:+50836411 Msa0210610:CDS
GTTAATTGAGGCTTCGAAGT+GGG 0.567258 2_1:+50836883 Msa0210610:exon
AGCATCTGGGGAGATACTGA+TGG 0.572323 2_1:-50836445 None:intergenic
GCATCTGGGGAGATACTGAT+GGG 0.583176 2_1:-50836444 None:intergenic
AATTGGCTCAGGCTCTTGCA+GGG 0.601314 2_1:+50836403 Msa0210610:CDS
CTTGGAACAATCAGCATCTG+GGG 0.606095 2_1:-50836457 None:intergenic
CCAGAGCAACATGCAGCTGG+TGG 0.627457 2_1:-50836282 None:intergenic
ATCAGCTGTATCAACATCTG+AGG 0.627591 2_1:+50836320 Msa0210610:CDS
TGTGTGAGCTATTTGGTCAG+TGG 0.644010 2_1:+50836249 Msa0210610:CDS
GCTTCGAAGTGGGAATTGGA+GGG 0.644556 2_1:+50836893 Msa0210610:three_prime_UTR
CTGACCAAATAGCTCACACA+AGG 0.667347 2_1:-50836246 None:intergenic
GGTTAATTGAGGCTTCGAAG+TGG 0.670085 2_1:+50836882 Msa0210610:exon
ATCTGGGGAGATACTGATGG+GGG 0.675132 2_1:-50836442 None:intergenic
CATCTGGGGAGATACTGATG+GGG 0.712150 2_1:-50836443 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATAAATAAGTAAATAA+AGG - chr2_1:50836837-50836856 None:intergenic 10.0%
!! AAAGATAAATAAGTAAATAA+AGG - chr2_1:50836837-50836856 None:intergenic 10.0%
!!! TTTAATGTATGATTTTTACT+TGG - chr2_1:50836478-50836497 None:intergenic 15.0%
!!! TGAAACAACTTTTTTTTTAT+AGG + chr2_1:50836624-50836643 Msa0210610:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG - chr2_1:50836731-50836750 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG - chr2_1:50836773-50836792 None:intergenic 15.0%
!!! TTTAATGTATGATTTTTACT+TGG - chr2_1:50836478-50836497 None:intergenic 15.0%
!!! TGAAACAACTTTTTTTTTAT+AGG + chr2_1:50836624-50836643 Msa0210610:intron 15.0%
!! TTCATTAATCAATTTGATAT+TGG - chr2_1:50836731-50836750 None:intergenic 15.0%
!! TTTGTAAAGCAAAATATAAA+AGG - chr2_1:50836773-50836792 None:intergenic 15.0%
!! TATTAAAATCAACTCACAAT+TGG + chr2_1:50836386-50836405 Msa0210610:CDS 20.0%
!!! ACTTTTTTTTTATAGGAGAT+TGG + chr2_1:50836631-50836650 Msa0210610:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG + chr2_1:50836795-50836814 Msa0210610:intron 20.0%
!! GAAAGAAAAATTATTAACCT+TGG - chr2_1:50836815-50836834 None:intergenic 20.0%
!! TATTAAAATCAACTCACAAT+TGG + chr2_1:50836386-50836405 Msa0210610:CDS 20.0%
!!! ACTTTTTTTTTATAGGAGAT+TGG + chr2_1:50836631-50836650 Msa0210610:intron 20.0%
!!! ATTAGCTTTTATTTTAACCA+AGG + chr2_1:50836795-50836814 Msa0210610:intron 20.0%
!! GAAAGAAAAATTATTAACCT+TGG - chr2_1:50836815-50836834 None:intergenic 20.0%
!!! TGAGTTGATTTTAATACTCT+TGG - chr2_1:50836382-50836401 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG + chr2_1:50836523-50836542 Msa0210610:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG + chr2_1:50836524-50836543 Msa0210610:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG + chr2_1:50836670-50836689 Msa0210610:intron 25.0%
! CAATTTGATATTGGAAAACT+TGG - chr2_1:50836722-50836741 None:intergenic 25.0%
!!! TGAGTTGATTTTAATACTCT+TGG - chr2_1:50836382-50836401 None:intergenic 25.0%
! TATGTTGATCCATAATGAAT+AGG + chr2_1:50836523-50836542 Msa0210610:intron 25.0%
! ATGTTGATCCATAATGAATA+GGG + chr2_1:50836524-50836543 Msa0210610:intron 25.0%
!! GCAACTTTAAGAATAAAACT+GGG + chr2_1:50836670-50836689 Msa0210610:intron 25.0%
! CAATTTGATATTGGAAAACT+TGG - chr2_1:50836722-50836741 None:intergenic 25.0%
!! GATTTTAATACTCTTGGAAG+TGG - chr2_1:50836376-50836395 None:intergenic 30.0%
CTTCGAGACTAATCTTTAAT+CGG + chr2_1:50836598-50836617 Msa0210610:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG + chr2_1:50836669-50836688 Msa0210610:intron 30.0%
!! GATTTTAATACTCTTGGAAG+TGG - chr2_1:50836376-50836395 None:intergenic 30.0%
CTTCGAGACTAATCTTTAAT+CGG + chr2_1:50836598-50836617 Msa0210610:intron 30.0%
! GGCAACTTTAAGAATAAAAC+TGG + chr2_1:50836669-50836688 Msa0210610:intron 30.0%
! CTTTGTTGCTTCAAATTCAG+AGG + chr2_1:50836185-50836204 Msa0210610:CDS 35.0%
TTCATGCAGTGATGTGATTA+AGG + chr2_1:50836215-50836234 Msa0210610:intron 35.0%
AGATTGGACTGCTTATCTAT+TGG + chr2_1:50836647-50836666 Msa0210610:intron 35.0%
GATTGGACTGCTTATCTATT+GGG + chr2_1:50836648-50836667 Msa0210610:intron 35.0%
!! ATTGCAGGATTGGTTAATTG+AGG + chr2_1:50836871-50836890 Msa0210610:intron 35.0%
! CTTTGTTGCTTCAAATTCAG+AGG + chr2_1:50836185-50836204 Msa0210610:CDS 35.0%
TTCATGCAGTGATGTGATTA+AGG + chr2_1:50836215-50836234 Msa0210610:intron 35.0%
AGATTGGACTGCTTATCTAT+TGG + chr2_1:50836647-50836666 Msa0210610:intron 35.0%
GATTGGACTGCTTATCTATT+GGG + chr2_1:50836648-50836667 Msa0210610:intron 35.0%
!! ATTGCAGGATTGGTTAATTG+AGG + chr2_1:50836871-50836890 Msa0210610:intron 35.0%
GAAACCTTGTGTGAGCTATT+TGG + chr2_1:50836242-50836261 Msa0210610:CDS 40.0%
ATCAGCTGTATCAACATCTG+AGG + chr2_1:50836320-50836339 Msa0210610:CDS 40.0%
! AATCAACTCACAATTGGCTC+AGG + chr2_1:50836392-50836411 Msa0210610:CDS 40.0%
ACTTGGAACAATCAGCATCT+GGG - chr2_1:50836461-50836480 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG - chr2_1:50836462-50836481 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG - chr2_1:50836535-50836554 None:intergenic 40.0%
GTTAATTGAGGCTTCGAAGT+GGG + chr2_1:50836883-50836902 Msa0210610:exon 40.0%
GAAACCTTGTGTGAGCTATT+TGG + chr2_1:50836242-50836261 Msa0210610:CDS 40.0%
ATCAGCTGTATCAACATCTG+AGG + chr2_1:50836320-50836339 Msa0210610:CDS 40.0%
! AATCAACTCACAATTGGCTC+AGG + chr2_1:50836392-50836411 Msa0210610:CDS 40.0%
ACTTGGAACAATCAGCATCT+GGG - chr2_1:50836461-50836480 None:intergenic 40.0%
TACTTGGAACAATCAGCATC+TGG - chr2_1:50836462-50836481 None:intergenic 40.0%
TGGCTACACCCTATTCATTA+TGG - chr2_1:50836535-50836554 None:intergenic 40.0%
GTTAATTGAGGCTTCGAAGT+GGG + chr2_1:50836883-50836902 Msa0210610:exon 40.0%
CTGACCAAATAGCTCACACA+AGG - chr2_1:50836249-50836268 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG + chr2_1:50836249-50836268 Msa0210610:CDS 45.0%
AGCTATTTGGTCAGTGGAAG+TGG + chr2_1:50836255-50836274 Msa0210610:CDS 45.0%
CTTGGAACAATCAGCATCTG+GGG - chr2_1:50836460-50836479 None:intergenic 45.0%
!! TGTAGCGATTTTGATACCCG+AGG - chr2_1:50836558-50836577 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG + chr2_1:50836856-50836875 Msa0210610:intron 45.0%
GGTTAATTGAGGCTTCGAAG+TGG + chr2_1:50836882-50836901 Msa0210610:exon 45.0%
CTGACCAAATAGCTCACACA+AGG - chr2_1:50836249-50836268 None:intergenic 45.0%
TGTGTGAGCTATTTGGTCAG+TGG + chr2_1:50836249-50836268 Msa0210610:CDS 45.0%
AGCTATTTGGTCAGTGGAAG+TGG + chr2_1:50836255-50836274 Msa0210610:CDS 45.0%
CTTGGAACAATCAGCATCTG+GGG - chr2_1:50836460-50836479 None:intergenic 45.0%
!! TGTAGCGATTTTGATACCCG+AGG - chr2_1:50836558-50836577 None:intergenic 45.0%
TTTGTTGCTGCGTCTATTGC+AGG + chr2_1:50836856-50836875 Msa0210610:intron 45.0%
GGTTAATTGAGGCTTCGAAG+TGG + chr2_1:50836882-50836901 Msa0210610:exon 45.0%
! AATTGGCTCAGGCTCTTGCA+GGG + chr2_1:50836403-50836422 Msa0210610:CDS 50.0%
ATCTGGGGAGATACTGATGG+GGG - chr2_1:50836445-50836464 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG - chr2_1:50836446-50836465 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG - chr2_1:50836447-50836466 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG - chr2_1:50836448-50836467 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG + chr2_1:50836538-50836557 Msa0210610:intron 50.0%
!! AGCGATTTTGATACCCGAGG+TGG - chr2_1:50836555-50836574 None:intergenic 50.0%
TGCTGCGTCTATTGCAGGAT+TGG + chr2_1:50836861-50836880 Msa0210610:intron 50.0%
TGAGGCTTCGAAGTGGGAAT+TGG + chr2_1:50836889-50836908 Msa0210610:three_prime_UTR 50.0%
GCTTCGAAGTGGGAATTGGA+GGG + chr2_1:50836893-50836912 Msa0210610:three_prime_UTR 50.0%
! AATTGGCTCAGGCTCTTGCA+GGG + chr2_1:50836403-50836422 Msa0210610:CDS 50.0%
ATCTGGGGAGATACTGATGG+GGG - chr2_1:50836445-50836464 None:intergenic 50.0%
CATCTGGGGAGATACTGATG+GGG - chr2_1:50836446-50836465 None:intergenic 50.0%
GCATCTGGGGAGATACTGAT+GGG - chr2_1:50836447-50836466 None:intergenic 50.0%
AGCATCTGGGGAGATACTGA+TGG - chr2_1:50836448-50836467 None:intergenic 50.0%
TGAATAGGGTGTAGCCACCT+CGG + chr2_1:50836538-50836557 Msa0210610:intron 50.0%
!! AGCGATTTTGATACCCGAGG+TGG - chr2_1:50836555-50836574 None:intergenic 50.0%
TGCTGCGTCTATTGCAGGAT+TGG + chr2_1:50836861-50836880 Msa0210610:intron 50.0%
TGAGGCTTCGAAGTGGGAAT+TGG + chr2_1:50836889-50836908 Msa0210610:three_prime_UTR 50.0%
GCTTCGAAGTGGGAATTGGA+GGG + chr2_1:50836893-50836912 Msa0210610:three_prime_UTR 50.0%
! CAATTGGCTCAGGCTCTTGC+AGG + chr2_1:50836402-50836421 Msa0210610:CDS 55.0%
GAATAGGGTGTAGCCACCTC+GGG + chr2_1:50836539-50836558 Msa0210610:intron 55.0%
GGCTTCGAAGTGGGAATTGG+AGG + chr2_1:50836892-50836911 Msa0210610:three_prime_UTR 55.0%
! CAATTGGCTCAGGCTCTTGC+AGG + chr2_1:50836402-50836421 Msa0210610:CDS 55.0%
GAATAGGGTGTAGCCACCTC+GGG + chr2_1:50836539-50836558 Msa0210610:intron 55.0%
GGCTTCGAAGTGGGAATTGG+AGG + chr2_1:50836892-50836911 Msa0210610:three_prime_UTR 55.0%
CCAGAGCAACATGCAGCTGG+TGG - chr2_1:50836285-50836304 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG + chr2_1:50836282-50836301 Msa0210610:CDS 60.0%
GCTCCAGAGCAACATGCAGC+TGG - chr2_1:50836288-50836307 None:intergenic 60.0%
CAGGCTCTTGCAGGGAACTG+TGG + chr2_1:50836411-50836430 Msa0210610:CDS 60.0%
CCAGAGCAACATGCAGCTGG+TGG - chr2_1:50836285-50836304 None:intergenic 60.0%
CCACCAGCTGCATGTTGCTC+TGG + chr2_1:50836282-50836301 Msa0210610:CDS 60.0%
GCTCCAGAGCAACATGCAGC+TGG - chr2_1:50836288-50836307 None:intergenic 60.0%
CAGGCTCTTGCAGGGAACTG+TGG + chr2_1:50836411-50836430 Msa0210610:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 50836141 50836933 50836141 ID=Msa0210610;Name=Msa0210610
chr2_1 mRNA 50836141 50836933 50836141 ID=Msa0210610-mRNA-1;Parent=Msa0210610;Name=Msa0210610-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.5|0.33|1|0|0|3|46|112
chr2_1 exon 50836141 50836227 50836141 ID=Msa0210610-mRNA-1:exon:14097;Parent=Msa0210610-mRNA-1
chr2_1 exon 50836237 50836478 50836237 ID=Msa0210610-mRNA-1:exon:14098;Parent=Msa0210610-mRNA-1
chr2_1 exon 50836878 50836933 50836878 ID=Msa0210610-mRNA-1:exon:14099;Parent=Msa0210610-mRNA-1
chr2_1 CDS 50836141 50836227 50836141 ID=Msa0210610-mRNA-1:cds;Parent=Msa0210610-mRNA-1
chr2_1 CDS 50836237 50836478 50836237 ID=Msa0210610-mRNA-1:cds;Parent=Msa0210610-mRNA-1
chr2_1 CDS 50836878 50836887 50836878 ID=Msa0210610-mRNA-1:cds;Parent=Msa0210610-mRNA-1
chr2_1 three_prime_UTR 50836888 50836933 50836888 ID=Msa0210610-mRNA-1:three_prime_utr;Parent=Msa0210610-mRNA-1
Gene Sequence

>Msa0210610

ATGAAATCTTCTTTAATTGCATTTTCTGCTGTTGTGCTTCTCATCTTTGTTGCTTCAAATTCAGAGGCTGCAATTTCATGCAGTGATGACTTGAAACCTTGTGTGAGCTATTTGGTCAGTGGAAGTGGACAACCACCAGCTGCATGTTGCTCTGGAGCTAAAGCTCTAGCATCAGCTGTATCAACATCTGAGGATAAAAAAGCTGCTTGCAACTGCATCAAATCCACTTCCAAGAGTATTAAAATCAACTCACAATTGGCTCAGGCTCTTGCAGGGAACTGTGGAATTAACACCCCCATCAGTATCTCCCCAGATGCTGATTGTTCCAAGATTGGTTAA

Protein sequence

>Msa0210610

MKSSLIAFSAVVLLIFVASNSEAAISCSDDLKPCVSYLVSGSGQPPAACCSGAKALASAVSTSEDKKAACNCIKSTSKSIKINSQLAQALAGNCGINTPISISPDADCSKIG*