AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0260900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0260900 MtrunA17_Chr2g0322391 93.058 605 29 4 1 595 1 602 0.0 1040
Msa0260900 MtrunA17_Chr4g0075201 49.597 496 179 15 75 551 36 479 1.21e-122 371
Msa0260900 MtrunA17_Chr2g0288871 48.343 513 187 19 75 551 62 532 2.68e-118 362
Msa0260900 MtrunA17_Chr4g0025051 43.333 570 233 16 80 587 35 576 2.78e-112 347
Msa0260900 MtrunA17_Chr4g0025061 41.156 571 226 19 58 556 36 568 1.32e-110 344
Msa0260900 MtrunA17_Chr4g0024391 41.700 494 208 13 162 583 1 486 3.67e-97 305
Msa0260900 MtrunA17_Chr3g0099641 40.664 482 207 14 75 548 34 444 1.29e-95 300
Msa0260900 MtrunA17_Chr7g0270831 37.209 516 186 18 76 574 26 420 1.96e-77 252
Msa0260900 MtrunA17_Chr2g0302391 35.236 403 187 13 159 547 66 408 5.45e-53 187
Msa0260900 MtrunA17_Chr7g0216891 31.120 482 231 18 166 573 45 499 1.95e-46 172
Msa0260900 MtrunA17_Chr4g0051181 42.748 262 124 7 326 562 227 487 1.62e-42 159
Msa0260900 MtrunA17_Chr4g0060271 37.500 272 134 7 151 402 60 315 4.25e-40 154
Msa0260900 MtrunA17_Chr5g0419241 84.615 65 7 1 308 372 46 107 1.82e-31 117
Msa0260900 MtrunA17_Chr4g0001361 31.140 228 100 8 319 545 139 310 5.26e-26 108
Msa0260900 MtrunA17_Chr3g0101131 38.122 181 83 5 252 404 297 476 7.18e-25 109
Msa0260900 MtrunA17_Chr5g0445311 44.628 121 56 3 294 404 306 425 2.23e-24 107
Msa0260900 MtrunA17_Chr3g0106491 54.430 79 35 1 324 402 347 424 5.27e-24 105
Msa0260900 MtrunA17_Chr3g0106491 47.619 63 31 2 342 404 198 258 4.91e-11 65.5
Msa0260900 MtrunA17_Chr3g0079691 37.423 163 77 3 246 404 415 556 1.19e-23 105
Msa0260900 MtrunA17_Chr3g0079691 42.683 82 44 3 343 423 281 360 3.07e-12 69.7
Msa0260900 MtrunA17_Chr1g0150271 39.516 124 62 3 282 402 48 161 1.64e-23 100
Msa0260900 MtrunA17_Chr1g0150281 48.889 90 46 0 327 416 125 214 3.22e-23 100
Msa0260900 MtrunA17_Chr5g0447701 51.724 87 41 1 316 402 226 311 3.66e-23 102
Msa0260900 MtrunA17_Chr3g0136961 52.632 76 36 0 325 400 121 196 4.43e-23 99.8
Msa0260900 MtrunA17_Chr7g0218081 50.575 87 42 1 318 404 386 471 4.67e-23 103
Msa0260900 MtrunA17_Chr7g0218081 52.459 61 27 2 342 402 228 286 1.85e-12 70.1
Msa0260900 MtrunA17_Chr4g0004911 53.165 79 36 1 324 402 318 395 1.11e-22 101
Msa0260900 MtrunA17_Chr4g0004911 40.000 75 42 2 328 402 172 243 1.04e-11 67.4
Msa0260900 MtrunA17_Chr7g0243891 53.571 84 38 1 321 404 353 435 1.42e-22 101
Msa0260900 MtrunA17_Chr5g0432161 52.381 84 39 1 321 404 400 482 1.45e-22 102
Msa0260900 MtrunA17_Chr5g0432161 35.714 112 60 4 291 402 203 302 4.58e-11 65.9
Msa0260900 MtrunA17_Chr4g0068541 51.948 77 36 1 326 402 192 267 2.04e-22 100
Msa0260900 MtrunA17_Chr7g0270191 46.739 92 42 2 326 417 129 213 6.32e-22 96.7
Msa0260900 MtrunA17_Chr5g0418191 50.617 81 39 1 324 404 302 381 7.71e-22 98.6
Msa0260900 MtrunA17_Chr5g0418191 49.180 61 29 2 342 402 151 209 1.22e-11 67.0
Msa0260900 MtrunA17_Chr5g0418781 47.126 87 45 1 313 399 95 180 1.92e-21 94.7
Msa0260900 MtrunA17_Chr7g0249311 51.250 80 38 1 325 404 375 453 3.37e-21 98.2
Msa0260900 MtrunA17_Chr7g0249311 47.692 65 32 2 338 402 175 237 7.66e-11 65.1
Msa0260900 MtrunA17_Chr2g0294231 49.351 77 38 1 326 402 187 262 3.54e-21 95.9
Msa0260900 MtrunA17_Chr4g0042831 49.383 81 40 1 324 404 260 339 3.98e-21 95.9
Msa0260900 MtrunA17_Chr4g0042831 40.741 81 45 2 342 422 145 222 3.06e-11 65.5
Msa0260900 MtrunA17_Chr7g0249301 50.617 81 39 1 324 404 274 353 4.71e-21 95.5
Msa0260900 MtrunA17_Chr7g0249301 47.692 65 32 2 338 402 58 120 4.06e-11 65.1
Msa0260900 MtrunA17_Chr1g0150801 50.685 73 35 1 327 399 87 158 7.89e-21 89.7
Msa0260900 MtrunA17_Chr7g0224801 50.704 71 34 1 331 401 82 151 2.21e-20 88.6
Msa0260900 MtrunA17_Chr1g0194481 40.000 115 65 2 326 437 142 255 3.09e-20 92.0
Msa0260900 MtrunA17_Chr2g0333851 30.085 236 87 9 320 545 142 309 3.15e-20 92.0
Msa0260900 MtrunA17_Chr4g0051881 51.351 74 35 1 329 402 165 237 6.05e-20 91.7
Msa0260900 MtrunA17_Chr1g0147881 47.561 82 43 0 326 407 246 327 9.97e-20 90.9
Msa0260900 MtrunA17_Chr3g0129361 46.067 89 46 2 325 413 127 213 1.26e-19 88.2
Msa0260900 MtrunA17_Chr8g0383351 45.556 90 46 3 325 413 142 229 7.88e-19 85.9
Msa0260900 MtrunA17_Chr7g0263531 43.529 85 47 1 318 402 337 420 1.34e-18 88.6
Msa0260900 MtrunA17_Chr7g0263531 50.000 60 28 2 343 402 190 247 1.69e-11 66.6
Msa0260900 MtrunA17_Chr8g0379461 54.930 71 31 1 333 402 66 136 1.45e-18 85.5
Msa0260900 MtrunA17_Chr4g0074001 50.649 77 37 1 326 402 343 418 1.79e-18 89.0
Msa0260900 MtrunA17_Chr8g0386241 40.659 91 53 1 315 405 76 165 2.17e-18 83.2
Msa0260900 MtrunA17_Chr5g0443131 48.649 74 37 1 329 402 144 216 2.20e-18 86.3
Msa0260900 MtrunA17_Chr8g0335811 44.444 81 44 1 320 400 132 211 4.22e-18 83.6
Msa0260900 MtrunA17_Chr1g0184691 47.436 78 41 0 326 403 277 354 4.87e-18 86.3
Msa0260900 MtrunA17_Chr8g0383251 44.595 74 40 1 329 402 95 167 5.13e-18 82.4
Msa0260900 MtrunA17_Chr4g0053281 46.835 79 42 0 326 404 250 328 6.75e-18 85.5
Msa0260900 MtrunA17_Chr5g0401821 47.368 76 40 0 326 401 183 258 1.15e-17 83.6
Msa0260900 MtrunA17_Chr7g0270051 38.384 99 55 1 312 404 17 115 1.38e-17 83.2
Msa0260900 MtrunA17_Chr2g0284321 54.839 62 28 0 342 403 35 96 1.52e-17 82.0
Msa0260900 MtrunA17_Chr2g0319961 51.429 70 34 0 333 402 71 140 1.78e-17 82.8
Msa0260900 MtrunA17_Chr4g0071521 48.000 75 39 0 326 400 229 303 2.15e-17 83.6
Msa0260900 MtrunA17_Chr3g0129321 45.946 74 39 1 329 402 105 177 8.19e-17 79.3
Msa0260900 MtrunA17_Chr7g0228941 47.887 71 37 0 329 399 39 109 1.07e-16 76.6
Msa0260900 MtrunA17_Chr2g0289181 44.156 77 43 0 337 413 9 85 1.28e-16 79.7
Msa0260900 MtrunA17_Chr1g0188941 44.156 77 42 1 326 402 35 110 1.42e-16 75.9
Msa0260900 MtrunA17_Chr7g0248721 45.000 80 42 2 320 399 119 196 2.68e-16 78.2
Msa0260900 MtrunA17_Chr3g0139021 45.946 74 40 0 326 399 298 371 4.23e-16 80.9
Msa0260900 MtrunA17_Chr7g0238921 35.484 155 89 5 258 403 32 184 1.30e-15 78.2
Msa0260900 MtrunA17_Chr6g0485431 36.774 155 79 6 266 402 61 214 3.17e-15 77.8
Msa0260900 MtrunA17_Chr2g0293481 45.833 72 39 0 343 414 268 339 4.96e-15 77.8
Msa0260900 MtrunA17_Chr8g0348311 50.000 64 32 0 339 402 152 215 6.12e-15 76.6
Msa0260900 MtrunA17_Chr7g0248691 42.683 82 35 1 333 402 133 214 1.87e-14 74.7
Msa0260900 MtrunA17_Chr1g0202461 40.659 91 52 1 339 429 258 346 4.72e-14 74.3
Msa0260900 MtrunA17_Chr1g0189391 42.466 73 40 2 329 400 84 155 1.23e-13 72.4
Msa0260900 MtrunA17_Chr3g0126391 48.684 76 32 2 329 397 115 190 1.67e-13 72.0
Msa0260900 MtrunA17_Chr8g0387501 45.161 62 34 0 341 402 128 189 1.21e-12 68.6
Msa0260900 MtrunA17_Chr7g0244971 34.579 107 67 2 318 421 98 204 1.73e-12 68.6
Msa0260900 MtrunA17_Chr7g0244981 36.449 107 65 2 318 421 97 203 3.68e-12 67.4
Msa0260900 MtrunA17_Chr8g0347251 36.036 111 56 3 302 397 83 193 3.84e-12 68.2
Msa0260900 MtrunA17_Chr4g0024401 41.964 112 43 5 58 165 28 121 1.56e-11 62.0
Msa0260900 MtrunA17_Chr2g0315361 40.541 74 42 1 326 397 132 205 3.29e-11 64.7
Msa0260900 MtrunA17_Chr5g0447711 36.782 87 54 1 316 402 204 289 3.43e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0260900 AT1G62300.1 49.455 459 184 10 127 564 121 552 1.73e-120 368
Msa0260900 AT4G04450.1 44.643 504 239 14 68 548 17 503 2.05e-109 338
Msa0260900 AT4G04450.2 48.210 419 193 10 144 548 1 409 2.68e-101 314
Msa0260900 AT4G22070.4 45.279 519 219 14 69 548 22 514 1.64e-100 316
Msa0260900 AT4G22070.3 45.279 519 219 14 69 548 22 514 1.64e-100 316
Msa0260900 AT4G22070.2 45.279 519 219 14 69 548 22 514 1.64e-100 316
Msa0260900 AT4G22070.1 45.279 519 219 14 69 548 22 514 1.64e-100 316
Msa0260900 AT4G01720.1 40.580 414 171 13 150 545 87 443 4.62e-66 224
Msa0260900 AT5G15130.1 32.809 445 230 16 161 550 35 465 5.29e-50 182
Msa0260900 AT5G15130.2 32.494 437 226 16 169 550 1 423 3.62e-48 176
Msa0260900 AT1G68150.1 38.849 278 113 9 165 435 97 324 1.73e-45 165
Msa0260900 AT1G18860.1 37.748 302 122 6 325 561 222 522 9.82e-42 158
Msa0260900 AT1G69810.1 33.739 329 187 9 161 477 25 334 3.80e-38 145
Msa0260900 AT1G69810.2 45.604 182 92 4 298 477 100 276 1.55e-36 139
Msa0260900 AT2G38470.1 44.628 121 65 2 324 444 344 462 1.87e-25 110
Msa0260900 AT2G38470.1 45.205 73 37 3 330 402 173 242 8.10e-11 65.1
Msa0260900 AT1G80840.1 30.603 232 98 7 319 545 118 291 4.05e-24 103
Msa0260900 AT4G26640.5 55.128 78 34 1 325 402 188 264 6.15e-24 104
Msa0260900 AT4G26640.6 55.128 78 34 1 325 402 188 264 6.15e-24 104
Msa0260900 AT4G26640.4 55.128 78 34 1 325 402 188 264 6.15e-24 104
Msa0260900 AT4G26640.3 55.128 78 34 1 325 402 188 264 6.15e-24 104
Msa0260900 AT4G26640.7 55.128 78 34 1 325 402 324 400 1.29e-23 105
Msa0260900 AT4G26640.1 55.128 78 34 1 325 402 292 368 1.51e-23 104
Msa0260900 AT4G26640.2 55.128 78 34 1 325 402 364 440 1.99e-23 104
Msa0260900 AT5G07100.2 41.880 117 61 3 290 402 87 200 1.05e-22 97.1
Msa0260900 AT5G07100.4 41.880 117 61 3 290 402 87 200 1.05e-22 97.1
Msa0260900 AT5G07100.5 41.880 117 61 3 290 402 87 200 1.05e-22 97.1
Msa0260900 AT5G07100.3 41.880 117 61 3 290 402 87 200 1.05e-22 97.1
Msa0260900 AT2G04880.2 47.674 86 44 1 321 406 262 346 1.53e-22 101
Msa0260900 AT2G04880.2 33.103 145 84 5 330 466 92 231 7.55e-11 65.1
Msa0260900 AT1G13960.1 53.165 79 36 1 324 402 391 468 2.00e-22 101
Msa0260900 AT1G13960.1 49.180 61 29 2 342 402 229 287 6.48e-12 68.6
Msa0260900 AT5G07100.1 40.625 128 66 4 279 402 172 293 2.06e-22 98.6
Msa0260900 AT1G13960.2 53.165 79 36 1 324 402 238 315 2.14e-22 99.8
Msa0260900 AT1G13960.2 49.180 61 29 2 342 402 76 134 1.20e-11 67.0
Msa0260900 AT2G04880.1 47.674 86 44 1 321 406 286 370 2.22e-22 101
Msa0260900 AT4G31800.2 58.462 65 27 0 341 405 110 174 5.42e-22 95.9
Msa0260900 AT5G56270.1 53.750 80 36 1 325 404 470 548 6.07e-22 100
Msa0260900 AT4G31800.1 58.462 65 27 0 341 405 175 239 6.37e-22 97.4
Msa0260900 AT1G29860.1 41.964 112 58 2 326 431 120 230 6.42e-22 96.7
Msa0260900 AT5G49520.1 49.351 77 38 1 326 402 205 280 7.50e-22 98.6
Msa0260900 AT5G26170.1 50.685 73 35 1 328 400 99 170 8.08e-22 93.2
Msa0260900 AT2G25000.2 42.708 96 55 0 311 406 72 167 1.02e-21 94.7
Msa0260900 AT2G25000.4 42.708 96 55 0 311 406 72 167 1.02e-21 94.7
Msa0260900 AT2G25000.3 42.708 96 55 0 311 406 94 189 1.09e-21 95.1
Msa0260900 AT2G25000.1 42.708 96 55 0 311 406 115 210 1.83e-21 95.1
Msa0260900 AT2G03340.1 48.276 87 44 1 318 404 391 476 2.67e-21 98.2
Msa0260900 AT1G69310.4 42.157 102 55 2 301 399 103 203 6.86e-21 93.6
Msa0260900 AT1G69310.2 42.157 102 55 2 301 399 103 203 6.86e-21 93.6
Msa0260900 AT1G69310.1 42.157 102 55 2 301 399 103 203 6.86e-21 93.6
Msa0260900 AT4G18170.1 51.948 77 36 1 326 402 156 231 8.71e-21 94.0
Msa0260900 AT3G01080.1 46.512 86 45 1 317 402 281 365 3.01e-20 94.0
Msa0260900 AT3G01080.2 46.512 86 45 1 317 402 319 403 3.02e-20 94.4
Msa0260900 AT2G47260.1 50.649 77 37 1 326 402 158 233 3.44e-20 92.8
Msa0260900 AT2G30250.1 50.000 80 39 1 325 404 311 389 4.13e-20 93.2
Msa0260900 AT4G26440.1 50.633 79 38 1 326 404 356 433 8.77e-20 93.6
Msa0260900 AT4G26440.1 50.000 62 29 2 342 403 178 237 3.89e-12 69.7
Msa0260900 AT3G01970.1 51.351 74 35 1 328 401 51 123 8.95e-20 86.7
Msa0260900 AT1G64000.1 41.346 104 56 2 316 419 88 186 2.17e-19 87.0
Msa0260900 AT5G46350.1 50.000 74 36 1 329 402 170 242 2.23e-19 90.1
Msa0260900 AT5G41570.1 50.000 82 39 2 319 399 74 154 2.87e-19 86.3
Msa0260900 AT4G39410.1 44.444 81 44 1 320 400 201 280 3.25e-19 89.0
Msa0260900 AT1G55600.1 43.820 89 49 1 316 404 281 368 7.99e-19 90.1
Msa0260900 AT5G13080.1 50.685 73 35 1 329 401 54 125 9.94e-19 83.6
Msa0260900 AT4G30935.1 50.649 77 37 1 326 402 315 390 2.15e-18 88.6
Msa0260900 AT2G46130.1 48.718 78 39 1 322 399 10 86 3.13e-18 80.9
Msa0260900 AT4G24240.1 50.000 76 38 0 326 401 265 340 3.87e-18 86.7
Msa0260900 AT2G23320.1 57.627 59 25 0 343 401 241 299 4.76e-18 85.9
Msa0260900 AT3G62340.1 50.000 78 38 1 329 406 105 181 7.11e-18 84.7
Msa0260900 AT2G30590.1 48.352 91 40 3 323 406 288 378 7.90e-18 86.3
Msa0260900 AT5G28650.1 49.383 81 35 2 326 400 240 320 8.54e-18 85.5
Msa0260900 AT2G44745.1 46.667 75 39 1 325 399 128 201 8.86e-18 83.2
Msa0260900 AT5G64810.1 47.887 71 36 1 329 399 97 166 9.97e-18 82.0
Msa0260900 AT2G24570.1 50.000 74 37 0 326 399 227 300 1.02e-17 85.1
Msa0260900 AT4G31550.2 48.649 74 38 0 326 399 229 302 2.15e-17 84.0
Msa0260900 AT4G31550.1 48.649 74 38 0 326 399 230 303 2.20e-17 84.0
Msa0260900 AT1G29280.1 47.143 70 37 0 333 402 65 134 3.78e-17 82.0
Msa0260900 AT3G04670.1 48.148 81 36 2 326 400 240 320 4.68e-17 83.2
Msa0260900 AT3G58710.1 43.820 89 49 1 315 402 43 131 3.65e-16 79.3
Msa0260900 AT3G58710.2 43.820 89 49 1 315 402 42 130 3.73e-16 79.3
Msa0260900 AT2G37260.3 49.333 75 34 2 337 408 264 337 4.94e-16 80.5
Msa0260900 AT2G37260.3 48.438 64 31 2 343 406 86 147 7.12e-13 70.9
Msa0260900 AT2G37260.2 49.333 75 34 2 337 408 262 335 5.12e-16 80.1
Msa0260900 AT2G37260.2 48.438 64 31 2 343 406 84 145 7.54e-13 70.5
Msa0260900 AT1G69310.3 39.216 102 54 3 301 399 103 199 5.37e-16 79.3
Msa0260900 AT2G37260.1 49.333 75 34 2 337 408 344 417 9.57e-16 80.1
Msa0260900 AT2G37260.1 48.438 64 31 2 343 406 166 227 1.06e-12 70.9
Msa0260900 AT4G01250.1 37.143 105 53 1 342 433 128 232 1.23e-15 78.6
Msa0260900 AT5G52830.1 50.746 67 33 0 336 402 159 225 1.47e-15 79.0
Msa0260900 AT4G23550.1 48.485 66 34 0 337 402 135 200 2.17e-15 77.8
Msa0260900 AT2G34830.1 44.737 76 41 1 328 402 200 275 4.18e-15 78.2
Msa0260900 AT1G30650.1 44.737 76 41 1 328 402 202 277 4.64e-15 78.2
Msa0260900 AT2G34830.2 44.737 76 41 1 328 402 209 284 5.47e-15 77.8
Msa0260900 AT2G46130.2 42.391 92 44 4 322 413 10 92 1.09e-14 70.9
Msa0260900 AT5G43290.1 43.421 76 41 2 329 403 100 174 2.66e-14 73.9
Msa0260900 AT4G31800.3 61.538 39 15 0 341 379 175 213 2.72e-12 67.4
Msa0260900 AT2G46400.1 41.772 79 44 1 321 397 83 161 8.10e-12 67.0
Msa0260900 AT2G21900.1 40.909 66 38 1 337 402 104 168 1.32e-11 64.7
Msa0260900 AT5G45050.2 47.541 61 32 0 342 402 1152 1212 1.92e-11 67.8
Msa0260900 AT5G45050.4 47.541 61 32 0 342 402 1041 1101 1.92e-11 67.8
Msa0260900 AT5G45050.1 47.541 61 32 0 342 402 1180 1240 1.98e-11 67.8

Find 120 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTTCCAAGACAATTTA+TGG 0.128951 2_2:-58872612 Msa0260900:CDS
ATTGCTCTTGTGAGGTAATT+TGG 0.146915 2_2:+58870173 None:intergenic
TTTGACTGATCTTGACTTCT+TGG 0.186141 2_2:+58872548 None:intergenic
GGTTATTAGGGAACTGGTTT+TGG 0.212904 2_2:+58870066 None:intergenic
CTGCTATTACTTCAATTATT+GGG 0.212910 2_2:-58869845 Msa0260900:CDS
GCTGCTATTACTTCAATTAT+TGG 0.246668 2_2:-58869846 Msa0260900:CDS
TGGTATAAGAAGCTTGAATT+TGG 0.269953 2_2:+58873841 None:intergenic
CTTGATGAGGAGAAACAAAA+TGG 0.281238 2_2:-58872647 Msa0260900:CDS
TTGAGAATTCTATTTGCTTA+TGG 0.285405 2_2:+58873821 None:intergenic
AAATGGGAATGGTGGAGTTT+TGG 0.297182 2_2:-58872630 Msa0260900:CDS
TAGCTACTCTACTGAGGAAT+TGG 0.308792 2_2:+58869701 None:intergenic
TTGCTCTTGTGAGGTAATTT+GGG 0.310844 2_2:+58870174 None:intergenic
CTAGTACCGTTATTATTGTT+TGG 0.316751 2_2:+58869813 None:intergenic
TTTGGTTTGGTGACTGAGTT+AGG 0.325584 2_2:+58870084 None:intergenic
AGGGAAATTGGAACTGGTTA+GGG 0.327267 2_2:+58870042 None:intergenic
ACATGCTTGATGAGGGTAAT+AGG 0.328005 2_2:-58872766 Msa0260900:CDS
ATTTCATGGTTGACTTGAAA+TGG 0.347073 2_2:+58873709 None:intergenic
GTTGCTATGCTTGAAGAATA+AGG 0.353526 2_2:+58870146 None:intergenic
AATAACGGTACTAGCAATAA+TGG 0.353919 2_2:-58869804 Msa0260900:CDS
GGGAAATTGGAACTGGTTAG+GGG 0.369219 2_2:+58870043 None:intergenic
AAACTTATATAGTCACAAAA+TGG 0.380131 2_2:-58873758 Msa0260900:CDS
GCTAAAGCTGCAGTGAAGTT+AGG 0.387680 2_2:+58869870 None:intergenic
AGGGTTGATGAGATATTTCA+TGG 0.388556 2_2:+58873695 None:intergenic
CCAAGACAATTTATGGAGCT+TGG 0.403211 2_2:-58872605 Msa0260900:CDS
AAGGGAAATTGGAACTGGTT+AGG 0.406882 2_2:+58870041 None:intergenic
TTTATGGCCAGTGGAGGGCT+TGG 0.407903 2_2:-58873883 Msa0260900:CDS
GGATGTCAATGGAGAAAGTA+TGG 0.409848 2_2:-58872189 Msa0260900:CDS
TTACACCGATCCTTCTCTCT+TGG 0.412872 2_2:-58873533 Msa0260900:CDS
TGCTATGTCTTCCTCCATCA+TGG 0.419633 2_2:+58872983 None:intergenic
TATCGATGCACCATGGCCTT+AGG 0.425405 2_2:-58872126 Msa0260900:CDS
GAAATTGGAACTGGTTAGGG+GGG 0.429542 2_2:+58870045 None:intergenic
GAGGGAAGGGAAATTGGAAC+TGG 0.429543 2_2:+58870036 None:intergenic
TGTACCATTTCCATAGCTGC+TGG 0.431979 2_2:+58870263 None:intergenic
TGCTTTGCCTCCAGCAGCTA+TGG 0.435611 2_2:-58870273 Msa0260900:CDS
GCTTGTTCAACATTTGTTTG+AGG 0.439934 2_2:+58872368 None:intergenic
AGTCCATCAGCACTTGTCAT+TGG 0.447523 2_2:+58870203 None:intergenic
TCCAATGTAACAGTTGGAAA+TGG 0.451153 2_2:+58870107 None:intergenic
AGACACAAGTTCTTCAACCT+TGG 0.451540 2_2:+58872496 None:intergenic
CGATAATAAGCTCGAGGACA+CGG 0.453138 2_2:+58872144 None:intergenic
ACAATAGCTAACAACACTAA+TGG 0.455086 2_2:-58869774 Msa0260900:CDS
TCCATTTCCAACTGTTACAT+TGG 0.455513 2_2:-58870108 Msa0260900:CDS
GCCTCCAGCAGCTATGGAAA+TGG 0.457149 2_2:-58870267 Msa0260900:CDS
ACAATTTATGGAGCTTGGAT+TGG 0.459747 2_2:-58872600 Msa0260900:CDS
TGAGGGAGGTGTAGAGGTAG+AGG 0.463067 2_2:+58873594 None:intergenic
TGAATTTGGATCTGAATCAA+TGG 0.468905 2_2:+58873855 None:intergenic
GGAAATTGGAACTGGTTAGG+GGG 0.470023 2_2:+58870044 None:intergenic
CACCTTCATTCTCTCAAGCT+CGG 0.471926 2_2:+58872808 None:intergenic
CCAAGCTCCATAAATTGTCT+TGG 0.475471 2_2:+58872605 None:intergenic
GAAGAGCAAAAGCAAGTGAT+TGG 0.479656 2_2:-58872677 Msa0260900:CDS
AACCCTTGCTTTCCTCATAG+TGG 0.482051 2_2:+58872340 None:intergenic
GCAGATGCATTGGATGAGTA+TGG 0.482506 2_2:-58872729 Msa0260900:CDS
TGAAAAGAAGGAATCCGATC+GGG 0.482638 2_2:-58872468 Msa0260900:CDS
AGCTACTCTACTGAGGAATT+GGG 0.491290 2_2:+58869702 None:intergenic
ATTTAGTGAGGGAGGTGTAG+AGG 0.493533 2_2:+58873588 None:intergenic
TGCTATTACTTCAATTATTG+GGG 0.494301 2_2:-58869844 Msa0260900:CDS
AGGTCTCATTTCATGATCAA+AGG 0.497698 2_2:+58873675 None:intergenic
AAGTCTAGTTCATTGATGAT+AGG 0.502804 2_2:+58873655 None:intergenic
TGTGTCTGATCATGAAAAGA+AGG 0.508518 2_2:-58872480 Msa0260900:CDS
GGAGAGTCCAAGCCCTCCAC+TGG 0.521274 2_2:+58873876 None:intergenic
GGTCTCATTTCATGATCAAA+GGG 0.522587 2_2:+58873676 None:intergenic
GCAGCTAAAACTCGATCTGC+AGG 0.523186 2_2:+58872425 None:intergenic
AGGTTGTCCTGTGAGAAAAC+AGG 0.526028 2_2:-58872106 Msa0260900:intron
AGCTCGATCCGAGGCTAACA+TGG 0.526714 2_2:-58872309 Msa0260900:intron
ATACTTATCACAACATATGA+AGG 0.527047 2_2:-58870308 Msa0260900:CDS
GATAATAAGCTCGAGGACAC+GGG 0.530098 2_2:+58872145 None:intergenic
TTGAAATTTATGGCCAGTGG+AGG 0.532791 2_2:-58873889 None:intergenic
GGATTGATCATTGCTTGTGT+TGG 0.535532 2_2:+58873004 None:intergenic
ACAACACATTGCAGATGCAT+TGG 0.536029 2_2:-58872739 Msa0260900:CDS
CATAATTTGCACAGGTACAA+AGG 0.536458 2_2:-58870349 Msa0260900:intron
ACTTTGAATTCCAAGAGAGA+AGG 0.536469 2_2:+58873523 None:intergenic
GAGAAAGTATGGACAGAAGA+TGG 0.537071 2_2:-58872178 Msa0260900:CDS
GGACCAATGACAAGTGCTGA+TGG 0.540715 2_2:-58870206 Msa0260900:CDS
GCTGCAAGAATGTTACTGTC+AGG 0.544674 2_2:-58870227 Msa0260900:CDS
TTGATGAGGAGAAACAAAAT+GGG 0.547963 2_2:-58872646 Msa0260900:CDS
GAGAAACAAAATGGGAATGG+TGG 0.550957 2_2:-58872638 Msa0260900:CDS
AGATCAATGATGGATGTCAA+TGG 0.557548 2_2:-58872200 Msa0260900:CDS
TCATTGAAATTTATGGCCAG+TGG 0.559553 2_2:-58873892 None:intergenic
CTGAGGAACATGCTTGATGA+GGG 0.560805 2_2:-58872773 Msa0260900:CDS
GAGTATGGTGCAAGACAAGA+AGG 0.561536 2_2:-58872714 Msa0260900:CDS
GAGCAAAAGCAAGTGATTGG+TGG 0.565607 2_2:-58872674 Msa0260900:CDS
GTTAGGTCCAATGTAACAGT+TGG 0.567146 2_2:+58870101 None:intergenic
ATGAAAAGAAGGAATCCGAT+CGG 0.570044 2_2:-58872469 Msa0260900:CDS
AGGCCACTATGAGGAAAGCA+AGG 0.570684 2_2:-58872343 Msa0260900:CDS
ACATCAAGTAACAATACCAT+TGG 0.571515 2_2:-58869744 Msa0260900:CDS
TAACAATGAGGAAGATTCCA+AGG 0.572334 2_2:-58872513 Msa0260900:CDS
TGAAATTTATGGCCAGTGGA+GGG 0.583495 2_2:-58873888 None:intergenic
GCACAGCCAAACAATAATAA+CGG 0.583839 2_2:-58869819 Msa0260900:CDS
CACAGGACAACCTAAGGCCA+TGG 0.587201 2_2:+58872116 None:intergenic
AAGCAATGATCAATCCATGA+TGG 0.591359 2_2:-58872997 Msa0260900:CDS
CTATGCTTGAAGAATAAGGA+AGG 0.594569 2_2:+58870150 None:intergenic
GGTGCAAGACAAGAAGGTAG+AGG 0.597094 2_2:-58872708 Msa0260900:CDS
GAATTCCAAGAGAGAAGGAT+CGG 0.597121 2_2:+58873528 None:intergenic
GAAGTGTACCATGTTAGCCT+CGG 0.600264 2_2:+58872301 None:intergenic
AGGCAGATGCAAGGTTGTGA+TGG 0.603220 2_2:+58873614 None:intergenic
AACAAGCTGAGGCCACTATG+AGG 0.607043 2_2:-58872352 Msa0260900:CDS
ATGATGATGAAGATTTAGTG+AGG 0.611103 2_2:+58873576 None:intergenic
ACTGAGGAACATGCTTGATG+AGG 0.611969 2_2:-58872774 Msa0260900:CDS
AGCCGAGCTTGAGAGAATGA+AGG 0.613065 2_2:-58872810 Msa0260900:CDS
AACAAATGTTGAACAAGCTG+AGG 0.613373 2_2:-58872363 Msa0260900:CDS
AAGGAAGGATTGCTCTTGTG+AGG 0.621106 2_2:+58870165 None:intergenic
TAGCATTAACTGTGTCAGCA+AGG 0.623284 2_2:+58869904 None:intergenic
TAGAGGTAGAGGCAGATGCA+AGG 0.626052 2_2:+58873605 None:intergenic
TGATGAAGATTTAGTGAGGG+AGG 0.630220 2_2:+58873580 None:intergenic
CGAGCTTGAGAGAATGAAGG+TGG 0.635577 2_2:-58872807 Msa0260900:CDS
TGATGATGAAGATTTAGTGA+GGG 0.636759 2_2:+58873577 None:intergenic
ACCATTTCCATAGCTGCTGG+AGG 0.637423 2_2:+58870266 None:intergenic
GAGGAGAAACAAAATGGGAA+TGG 0.637776 2_2:-58872641 Msa0260900:CDS
TATGGACAGAAGATGGCGAA+AGG 0.642826 2_2:-58872171 Msa0260900:CDS
CAATGATCAATCCATGATGG+AGG 0.645307 2_2:-58872994 Msa0260900:CDS
AGACAAAAGAGCTAAGCTTG+AGG 0.649723 2_2:-58872949 Msa0260900:intron
TTCAGTTAGAGCTCGATCCG+AGG 0.654330 2_2:-58872318 Msa0260900:CDS
GGCCACTATGAGGAAAGCAA+GGG 0.654709 2_2:-58872342 Msa0260900:CDS
ATTACTTGCAAATAACAATG+AGG 0.658433 2_2:-58872525 Msa0260900:CDS
AGCTTATTATCGATGCACCA+TGG 0.661870 2_2:-58872133 Msa0260900:CDS
GAAAAGAAGGAATCCGATCG+GGG 0.662543 2_2:-58872467 Msa0260900:CDS
ACTGTGTCAGCAAGGTTCTG+TGG 0.667152 2_2:+58869912 None:intergenic
GATTGGTGGAAAACTTGATG+AGG 0.667331 2_2:-58872660 Msa0260900:CDS
GTGCATCGATAATAAGCTCG+AGG 0.678750 2_2:+58872138 None:intergenic
AGGTGGAGAATCATCAACTG+AGG 0.706865 2_2:-58872790 Msa0260900:CDS
AATGTCTGACCAAATACCTG+AGG 0.730527 2_2:+58870002 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAGTGATATTATATATA+TGG + chr2_2:58870277-58870296 None:intergenic 10.0%
!! TTTCAAAAAAAAAATGAAAT+TGG + chr2_2:58872531-58872550 None:intergenic 10.0%
!! ACTATTAGAAAACATTAAAT+TGG - chr2_2:58870290-58870309 Msa0260900:CDS 15.0%
!!! ATTTTCTCATTACTAAATTT+TGG - chr2_2:58870374-58870393 Msa0260900:intron 15.0%
!!! TAATTTCTATAAACTTTTTC+AGG + chr2_2:58871935-58871954 None:intergenic 15.0%
!! AATAAAGACAAAAGATAATT+TGG - chr2_2:58872327-58872346 Msa0260900:CDS 15.0%
!! CATCTTAAAAATAAAAATGA+CGG + chr2_2:58872504-58872523 None:intergenic 15.0%
!!! GTCATTTTTATTTTTAAGAT+GGG - chr2_2:58872503-58872522 Msa0260900:CDS 15.0%
!!! ACTTTTTTTTGTAAACATTT+TGG - chr2_2:58872767-58872786 Msa0260900:CDS 15.0%
!!! TAATTTCAGTAAACTTTTTT+AGG + chr2_2:58872876-58872895 None:intergenic 15.0%
!! AAACTTATATAGTCACAAAA+TGG - chr2_2:58869826-58869845 Msa0260900:CDS 20.0%
!! ATTGCAATATCAATAACATA+TGG + chr2_2:58870167-58870186 None:intergenic 20.0%
!! TATATATATGGAATACCAAA+GGG + chr2_2:58870265-58870284 None:intergenic 20.0%
!! TTATATATATGGAATACCAA+AGG + chr2_2:58870266-58870285 None:intergenic 20.0%
!!! AACAAATTTTCCCATAAAAA+AGG - chr2_2:58870324-58870343 Msa0260900:CDS 20.0%
!!! ACAAATTTTCCCATAAAAAA+GGG - chr2_2:58870325-58870344 Msa0260900:CDS 20.0%
!!! TTTTTACTTCCCTTTTTTAT+GGG + chr2_2:58870337-58870356 None:intergenic 20.0%
!!! TTTTTTACTTCCCTTTTTTA+TGG + chr2_2:58870338-58870357 None:intergenic 20.0%
!!! TATTTAATTTGTTTGGTGAA+TGG - chr2_2:58870401-58870420 Msa0260900:intron 20.0%
!! TAAATGTTAGCTATAATAGT+TGG - chr2_2:58871515-58871534 Msa0260900:intron 20.0%
!!! CTGTTTTATATTGACTTATT+TGG + chr2_2:58872031-58872050 None:intergenic 20.0%
!!! GTTACTGTAGTTTAATATTT+TGG + chr2_2:58872077-58872096 None:intergenic 20.0%
!!! TTATATCTTCCATTTTATTC+TGG + chr2_2:58872149-58872168 None:intergenic 20.0%
!! TGTTTATATTGTCATTAAAC+TGG - chr2_2:58872193-58872212 Msa0260900:CDS 20.0%
!! AAAAGATAATTTGGACATTA+GGG - chr2_2:58872336-58872355 Msa0260900:CDS 20.0%
!!! CGTCATTTTTATTTTTAAGA+TGG - chr2_2:58872502-58872521 Msa0260900:CDS 20.0%
!!! TAACTTTTTATTTAGAGCTT+AGG - chr2_2:58872555-58872574 Msa0260900:CDS 20.0%
!! TATACAAACACGTTTAAAAA+AGG + chr2_2:58872667-58872686 None:intergenic 20.0%
!! TTTAAACGTGTTTGTATAAA+TGG - chr2_2:58872669-58872688 Msa0260900:CDS 20.0%
!!! TTTTTTTGTAAACATTTTGG+TGG - chr2_2:58872770-58872789 Msa0260900:CDS 20.0%
!!! TAATTCGGGTTTTATAAAAA+TGG + chr2_2:58873165-58873184 None:intergenic 20.0%
! TTGAGAATTCTATTTGCTTA+TGG + chr2_2:58869766-58869785 None:intergenic 25.0%
!!! AGATTTGTTTTTGTTGTTTG+TGG + chr2_2:58869855-58869874 None:intergenic 25.0%
!!! TGGTTGCTATTTAATTTGTT+TGG - chr2_2:58870394-58870413 Msa0260900:intron 25.0%
! GCACATATTATATATGACAT+TGG - chr2_2:58870500-58870519 Msa0260900:intron 25.0%
! TTAAATGAATAATCATCGAG+TGG - chr2_2:58870712-58870731 Msa0260900:intron 25.0%
! ATTACTTGCAAATAACAATG+AGG - chr2_2:58871059-58871078 Msa0260900:intron 25.0%
!! GAAGAATGAAATGTTTGTTT+TGG - chr2_2:58871324-58871343 Msa0260900:intron 25.0%
! CAATAATATCCAATCAATCA+TGG - chr2_2:58871745-58871764 Msa0260900:intron 25.0%
! TAAAACTGTTTATCCAAACA+TGG + chr2_2:58871805-58871824 None:intergenic 25.0%
! AAAGACTTATGAGACTATTT+GGG + chr2_2:58871992-58872011 None:intergenic 25.0%
!! GACAATATAAACATGCTTTT+AGG + chr2_2:58872186-58872205 None:intergenic 25.0%
! TTATATTGTCATTAAACTGG+AGG - chr2_2:58872196-58872215 Msa0260900:CDS 25.0%
! TCGATATAATCAAACTCTTA+AGG + chr2_2:58872279-58872298 None:intergenic 25.0%
! TTATATCGATGCTTGTTTAA+CGG - chr2_2:58872290-58872309 Msa0260900:intron 25.0%
! CAAAAGATAATTTGGACATT+AGG - chr2_2:58872335-58872354 Msa0260900:CDS 25.0%
! AAAGATAATTTGGACATTAG+GGG - chr2_2:58872337-58872356 Msa0260900:CDS 25.0%
! TCAAAAAAATTGAGCAATGT+GGG + chr2_2:58872390-58872409 None:intergenic 25.0%
! ATCAAAAAAATTGAGCAATG+TGG + chr2_2:58872391-58872410 None:intergenic 25.0%
!!! GCTCAATTTTTTTGATTGTT+TGG - chr2_2:58872395-58872414 Msa0260900:CDS 25.0%
! TTAGGTTTATACGCATTATT+TGG - chr2_2:58872573-58872592 Msa0260900:CDS 25.0%
! TAGGTTTATACGCATTATTT+GGG - chr2_2:58872574-58872593 Msa0260900:CDS 25.0%
! ACGTTTAAAAAAGGTAGAAA+AGG + chr2_2:58872658-58872677 None:intergenic 25.0%
!! GGATTACTACAATTGTATTT+TGG + chr2_2:58872728-58872747 None:intergenic 25.0%
! AGTTGAAATTCGCTTATTAT+GGG + chr2_2:58872750-58872769 None:intergenic 25.0%
! AAGTTGAAATTCGCTTATTA+TGG + chr2_2:58872751-58872770 None:intergenic 25.0%
! TTTATAAAAATGGTTCACGA+CGG + chr2_2:58873155-58873174 None:intergenic 25.0%
!! ACAGCTTGATAACTTTAATT+CGG + chr2_2:58873180-58873199 None:intergenic 25.0%
! ATACTTATCACAACATATGA+AGG - chr2_2:58873276-58873295 Msa0260900:intron 25.0%
!! TTGAACCAATCAAAATTTTC+AGG - chr2_2:58873606-58873625 Msa0260900:CDS 25.0%
! CTGCTATTACTTCAATTATT+GGG - chr2_2:58873739-58873758 Msa0260900:CDS 25.0%
! TGCTATTACTTCAATTATTG+GGG - chr2_2:58873740-58873759 Msa0260900:CDS 25.0%
!!! AATTGGGATTGTTGATTTTA+GGG + chr2_2:58873869-58873888 None:intergenic 25.0%
TGAATTTGGATCTGAATCAA+TGG + chr2_2:58869732-58869751 None:intergenic 30.0%
TGGTATAAGAAGCTTGAATT+TGG + chr2_2:58869746-58869765 None:intergenic 30.0%
!! ATTTCATGGTTGACTTGAAA+TGG + chr2_2:58869878-58869897 None:intergenic 30.0%
! AAGTCTAGTTCATTGATGAT+AGG + chr2_2:58869932-58869951 None:intergenic 30.0%
! TGATGATGAAGATTTAGTGA+GGG + chr2_2:58870010-58870029 None:intergenic 30.0%
! ATGATGATGAAGATTTAGTG+AGG + chr2_2:58870011-58870030 None:intergenic 30.0%
TCACTATTGTATAAACCATG+TGG - chr2_2:58870126-58870145 Msa0260900:CDS 30.0%
TTGATGAGGAGAAACAAAAT+GGG - chr2_2:58870938-58870957 Msa0260900:intron 30.0%
! TTTGGTTCCAAGACAATTTA+TGG - chr2_2:58870972-58870991 Msa0260900:intron 30.0%
! CTTTTGTATCAGATCAATGA+TGG - chr2_2:58871374-58871393 Msa0260900:intron 30.0%
AGCATGCATATAAGATCAAT+TGG + chr2_2:58871628-58871647 None:intergenic 30.0%
! TAGTAAAAGTACATGAGCTA+TGG + chr2_2:58871677-58871696 None:intergenic 30.0%
AATTACCTGAAAAGAGCTTA+TGG - chr2_2:58871950-58871969 Msa0260900:intron 30.0%
ATTACCTGAAAAGAGCTTAT+GGG - chr2_2:58871951-58871970 Msa0260900:intron 30.0%
CAAAGACTTATGAGACTATT+TGG + chr2_2:58871993-58872012 None:intergenic 30.0%
ACACTCAAACCAGAATAAAA+TGG - chr2_2:58872137-58872156 Msa0260900:CDS 30.0%
AAGATAATTTGGACATTAGG+GGG - chr2_2:58872338-58872357 Msa0260900:CDS 30.0%
GTTGAAATTCGCTTATTATG+GGG + chr2_2:58872749-58872768 None:intergenic 30.0%
AATTACCTGAAATGAGCTTA+TGG - chr2_2:58872891-58872910 Msa0260900:intron 30.0%
AAGCACTATTGAGACTATTT+GGG + chr2_2:58872932-58872951 None:intergenic 30.0%
TAAGCACTATTGAGACTATT+TGG + chr2_2:58872933-58872952 None:intergenic 30.0%
!!! TTTTTTACGTCTCTATGCTA+AGG + chr2_2:58873103-58873122 None:intergenic 30.0%
! CAGCTTGATAACTTTAATTC+GGG + chr2_2:58873179-58873198 None:intergenic 30.0%
! ACATGTAACATAATTTGCAC+AGG - chr2_2:58873227-58873246 Msa0260900:intron 30.0%
!! GAAGTCCTGAAAATTTTGAT+TGG + chr2_2:58873614-58873633 None:intergenic 30.0%
GCTGCTATTACTTCAATTAT+TGG - chr2_2:58873738-58873757 Msa0260900:CDS 30.0%
CTAGTACCGTTATTATTGTT+TGG + chr2_2:58873774-58873793 None:intergenic 30.0%
AATAACGGTACTAGCAATAA+TGG - chr2_2:58873780-58873799 Msa0260900:CDS 30.0%
ACAATAGCTAACAACACTAA+TGG - chr2_2:58873810-58873829 Msa0260900:CDS 30.0%
ACATCAAGTAACAATACCAT+TGG - chr2_2:58873840-58873859 Msa0260900:CDS 30.0%
!!! GAATTGGGATTGTTGATTTT+AGG + chr2_2:58873870-58873889 None:intergenic 30.0%
!!! TTTTTGAGGTTGTTGTGTTG+AGG + chr2_2:58869796-58869815 None:intergenic 35.0%
ACAACCTCAAAAACTTCAAG+TGG - chr2_2:58869803-58869822 Msa0260900:CDS 35.0%
! GTTTCCACTTGAAGTTTTTG+AGG + chr2_2:58869810-58869829 None:intergenic 35.0%
!! AGGGTTGATGAGATATTTCA+TGG + chr2_2:58869892-58869911 None:intergenic 35.0%
GGTCTCATTTCATGATCAAA+GGG + chr2_2:58869911-58869930 None:intergenic 35.0%
AGGTCTCATTTCATGATCAA+AGG + chr2_2:58869912-58869931 None:intergenic 35.0%
ACTTTGAATTCCAAGAGAGA+AGG + chr2_2:58870064-58870083 None:intergenic 35.0%
AACATATGGCCACAATATCA+AGG + chr2_2:58870153-58870172 None:intergenic 35.0%
CAAATGAGTTAATCACCCTT+TGG - chr2_2:58870247-58870266 Msa0260900:CDS 35.0%
AAGCAATGATCAATCCATGA+TGG - chr2_2:58870587-58870606 Msa0260900:intron 35.0%
CTTGATGAGGAGAAACAAAA+TGG - chr2_2:58870937-58870956 Msa0260900:intron 35.0%
ACAATTTATGGAGCTTGGAT+TGG - chr2_2:58870984-58871003 Msa0260900:intron 35.0%
TTTGACTGATCTTGACTTCT+TGG + chr2_2:58871039-58871058 None:intergenic 35.0%
TAACAATGAGGAAGATTCCA+AGG - chr2_2:58871071-58871090 Msa0260900:intron 35.0%
TGTGTCTGATCATGAAAAGA+AGG - chr2_2:58871104-58871123 Msa0260900:intron 35.0%
ATGAAAAGAAGGAATCCGAT+CGG - chr2_2:58871115-58871134 Msa0260900:intron 35.0%
GCTTGTTCAACATTTGTTTG+AGG + chr2_2:58871219-58871238 None:intergenic 35.0%
AACAAATGTTGAACAAGCTG+AGG - chr2_2:58871221-58871240 Msa0260900:intron 35.0%
!! AGATCAATGATGGATGTCAA+TGG - chr2_2:58871384-58871403 Msa0260900:intron 35.0%
! TAACATACCTGTTTTCTCAC+AGG + chr2_2:58871488-58871507 None:intergenic 35.0%
GGCACTGTTATAATATGTCA+TGG - chr2_2:58871545-58871564 Msa0260900:intron 35.0%
! AGCTTGAGATTGATTTAGCT+AGG - chr2_2:58871709-58871728 Msa0260900:intron 35.0%
TATAAAACAGACCCCTAATG+AGG - chr2_2:58872041-58872060 Msa0260900:intron 35.0%
ACTACAGTAACAACCAAAGT+CGG - chr2_2:58872086-58872105 Msa0260900:intron 35.0%
ATTGTCATTAAACTGGAGGT+TGG - chr2_2:58872200-58872219 Msa0260900:CDS 35.0%
! TCAAACTCTTAAGGTGCTTT+CGG + chr2_2:58872270-58872289 None:intergenic 35.0%
ACCATTTGAGTCCAATGATT+TGG - chr2_2:58872802-58872821 Msa0260900:CDS 35.0%
ACCAAATCATTGGACTCAAA+TGG + chr2_2:58872806-58872825 None:intergenic 35.0%
!! TTTGAGTCCAATGATTTGGT+TGG - chr2_2:58872806-58872825 Msa0260900:CDS 35.0%
ACTTTGACCAACCAAATCAT+TGG + chr2_2:58872816-58872835 None:intergenic 35.0%
! CATAATTTGCACAGGTACAA+AGG - chr2_2:58873235-58873254 Msa0260900:intron 35.0%
TTGCTCTTGTGAGGTAATTT+GGG + chr2_2:58873413-58873432 None:intergenic 35.0%
ATTGCTCTTGTGAGGTAATT+TGG + chr2_2:58873414-58873433 None:intergenic 35.0%
CTATGCTTGAAGAATAAGGA+AGG + chr2_2:58873437-58873456 None:intergenic 35.0%
GTTGCTATGCTTGAAGAATA+AGG + chr2_2:58873441-58873460 None:intergenic 35.0%
TCCATTTCCAACTGTTACAT+TGG - chr2_2:58873476-58873495 Msa0260900:intron 35.0%
TCCAATGTAACAGTTGGAAA+TGG + chr2_2:58873480-58873499 None:intergenic 35.0%
!! AATTTTGAGGGAAGGGAAAT+TGG + chr2_2:58873557-58873576 None:intergenic 35.0%
! AATTTTCTGCCTCAGGTATT+TGG - chr2_2:58873573-58873592 Msa0260900:CDS 35.0%
GCACAGCCAAACAATAATAA+CGG - chr2_2:58873765-58873784 Msa0260900:CDS 35.0%
TGATGAAGATTTAGTGAGGG+AGG + chr2_2:58870007-58870026 None:intergenic 40.0%
GAATTCCAAGAGAGAAGGAT+CGG + chr2_2:58870059-58870078 None:intergenic 40.0%
GGATTGATCATTGCTTGTGT+TGG + chr2_2:58870583-58870602 None:intergenic 40.0%
CAATGATCAATCCATGATGG+AGG - chr2_2:58870590-58870609 Msa0260900:intron 40.0%
AGACAAAAGAGCTAAGCTTG+AGG - chr2_2:58870635-58870654 Msa0260900:intron 40.0%
ACATGCTTGATGAGGGTAAT+AGG - chr2_2:58870818-58870837 Msa0260900:intron 40.0%
ACAACACATTGCAGATGCAT+TGG - chr2_2:58870845-58870864 Msa0260900:intron 40.0%
GAAGAGCAAAAGCAAGTGAT+TGG - chr2_2:58870907-58870926 Msa0260900:intron 40.0%
GATTGGTGGAAAACTTGATG+AGG - chr2_2:58870924-58870943 Msa0260900:intron 40.0%
GAGGAGAAACAAAATGGGAA+TGG - chr2_2:58870943-58870962 Msa0260900:intron 40.0%
GAGAAACAAAATGGGAATGG+TGG - chr2_2:58870946-58870965 Msa0260900:intron 40.0%
!! AAATGGGAATGGTGGAGTTT+TGG - chr2_2:58870954-58870973 Msa0260900:intron 40.0%
CCAAGCTCCATAAATTGTCT+TGG + chr2_2:58870982-58871001 None:intergenic 40.0%
CCAAGACAATTTATGGAGCT+TGG - chr2_2:58870979-58870998 Msa0260900:intron 40.0%
AGACACAAGTTCTTCAACCT+TGG + chr2_2:58871091-58871110 None:intergenic 40.0%
TGAAAAGAAGGAATCCGATC+GGG - chr2_2:58871116-58871135 Msa0260900:intron 40.0%
GGATGTCAATGGAGAAAGTA+TGG - chr2_2:58871395-58871414 Msa0260900:intron 40.0%
! GAGAAAGTATGGACAGAAGA+TGG - chr2_2:58871406-58871425 Msa0260900:intron 40.0%
AGCTTATTATCGATGCACCA+TGG - chr2_2:58871451-58871470 Msa0260900:intron 40.0%
! TTTTCTCACAGGACAACCTA+AGG + chr2_2:58871477-58871496 None:intergenic 40.0%
!! GCTATAATAGTTGGTGACAC+AGG - chr2_2:58871524-58871543 Msa0260900:intron 40.0%
CAGAGTTGAGAACTCAAAAC+AGG + chr2_2:58871590-58871609 None:intergenic 40.0%
AAACGTGTGCCATGATTGAT+TGG + chr2_2:58871757-58871776 None:intergenic 40.0%
GTCTTCAACATTTCGATGCT+AGG - chr2_2:58871779-58871798 Msa0260900:intron 40.0%
CTACAGTAACAACCAAAGTC+GGG - chr2_2:58872087-58872106 Msa0260900:intron 40.0%
! AACTGGAGGTTGGAGTTTTA+AGG - chr2_2:58872210-58872229 Msa0260900:intron 40.0%
ACATGCCATAAGCTCATTTC+AGG + chr2_2:58872899-58872918 None:intergenic 40.0%
ACTAAAACAGACCCCTAATG+AGG - chr2_2:58872981-58873000 Msa0260900:CDS 40.0%
ACTTCAACCTACCTCATTAG+GGG + chr2_2:58872995-58873014 None:intergenic 40.0%
GACTTCAACCTACCTCATTA+GGG + chr2_2:58872996-58873015 None:intergenic 40.0%
TGACTTCAACCTACCTCATT+AGG + chr2_2:58872997-58873016 None:intergenic 40.0%
ACTGAAGTAACGACCAAAGT+CGG - chr2_2:58873026-58873045 Msa0260900:CDS 40.0%
GTTAGGTCCAATGTAACAGT+TGG + chr2_2:58873486-58873505 None:intergenic 40.0%
!! TTTGGTTTGGTGACTGAGTT+AGG + chr2_2:58873503-58873522 None:intergenic 40.0%
!!! TTAGGGAACTGGTTTTGGTT+TGG + chr2_2:58873516-58873535 None:intergenic 40.0%
!! GGTTATTAGGGAACTGGTTT+TGG + chr2_2:58873521-58873540 None:intergenic 40.0%
AGGGAAATTGGAACTGGTTA+GGG + chr2_2:58873545-58873564 None:intergenic 40.0%
AAGGGAAATTGGAACTGGTT+AGG + chr2_2:58873546-58873565 None:intergenic 40.0%
!! GGCAGAAAATTTTGAGGGAA+GGG + chr2_2:58873564-58873583 None:intergenic 40.0%
!! AGGCAGAAAATTTTGAGGGA+AGG + chr2_2:58873565-58873584 None:intergenic 40.0%
! CCTGAGGCAGAAAATTTTGA+GGG + chr2_2:58873569-58873588 None:intergenic 40.0%
! ACCTGAGGCAGAAAATTTTG+AGG + chr2_2:58873570-58873589 None:intergenic 40.0%
AATGTCTGACCAAATACCTG+AGG + chr2_2:58873585-58873604 None:intergenic 40.0%
TAGCATTAACTGTGTCAGCA+AGG + chr2_2:58873683-58873702 None:intergenic 40.0%
!!! GTTGATTTTAGGGCTTCCAA+TGG + chr2_2:58873859-58873878 None:intergenic 40.0%
! ATTTAGTGAGGGAGGTGTAG+AGG + chr2_2:58869999-58870018 None:intergenic 45.0%
TTACACCGATCCTTCTCTCT+TGG - chr2_2:58870051-58870070 Msa0260900:CDS 45.0%
CCACAATATCAAGGTCCACA+TGG + chr2_2:58870144-58870163 None:intergenic 45.0%
CCATGTGGACCTTGATATTG+TGG - chr2_2:58870141-58870160 Msa0260900:CDS 45.0%
TGCTATGTCTTCCTCCATCA+TGG + chr2_2:58870604-58870623 None:intergenic 45.0%
CACCTTCATTCTCTCAAGCT+CGG + chr2_2:58870779-58870798 None:intergenic 45.0%
AGGTGGAGAATCATCAACTG+AGG - chr2_2:58870794-58870813 Msa0260900:intron 45.0%
ACTGAGGAACATGCTTGATG+AGG - chr2_2:58870810-58870829 Msa0260900:intron 45.0%
CTGAGGAACATGCTTGATGA+GGG - chr2_2:58870811-58870830 Msa0260900:intron 45.0%
GCAGATGCATTGGATGAGTA+TGG - chr2_2:58870855-58870874 Msa0260900:intron 45.0%
GAGTATGGTGCAAGACAAGA+AGG - chr2_2:58870870-58870889 Msa0260900:intron 45.0%
GAGCAAAAGCAAGTGATTGG+TGG - chr2_2:58870910-58870929 Msa0260900:intron 45.0%
GAAAAGAAGGAATCCGATCG+GGG - chr2_2:58871117-58871136 Msa0260900:intron 45.0%
! GCTTCTTTCTTTTCCCCGAT+CGG + chr2_2:58871133-58871152 None:intergenic 45.0%
AACCCTTGCTTTCCTCATAG+TGG + chr2_2:58871247-58871266 None:intergenic 45.0%
! GAAGTGTACCATGTTAGCCT+CGG + chr2_2:58871286-58871305 None:intergenic 45.0%
TATGGACAGAAGATGGCGAA+AGG - chr2_2:58871413-58871432 Msa0260900:intron 45.0%
GATAATAAGCTCGAGGACAC+GGG + chr2_2:58871442-58871461 None:intergenic 45.0%
CGATAATAAGCTCGAGGACA+CGG + chr2_2:58871443-58871462 None:intergenic 45.0%
! GTGCATCGATAATAAGCTCG+AGG + chr2_2:58871449-58871468 None:intergenic 45.0%
AGGTTGTCCTGTGAGAAAAC+AGG - chr2_2:58871478-58871497 Msa0260900:intron 45.0%
TTTCGATGCTAGGCCATGTT+TGG - chr2_2:58871789-58871808 Msa0260900:intron 45.0%
ACCTGAAAAGAGCTTATGGG+TGG - chr2_2:58871954-58871973 Msa0260900:intron 45.0%
CCTGAAAAGAGCTTATGGGT+GGG - chr2_2:58871955-58871974 Msa0260900:intron 45.0%
AAACAGACCCCTAATGAGGT+AGG - chr2_2:58872045-58872064 Msa0260900:intron 45.0%
GCTTCAACCTACCTCATTAG+GGG + chr2_2:58872055-58872074 None:intergenic 45.0%
GGCTTCAACCTACCTCATTA+GGG + chr2_2:58872056-58872075 None:intergenic 45.0%
TGGCTTCAACCTACCTCATT+AGG + chr2_2:58872057-58872076 None:intergenic 45.0%
AAACAGACCCCTAATGAGGT+AGG - chr2_2:58872045-58872064 Msa0260900:intron 45.0%
CTGAAGTAACGACCAAAGTC+GGG - chr2_2:58873027-58873046 Msa0260900:CDS 45.0%
CAAGAATTGTGACCCGACTT+TGG + chr2_2:58873042-58873061 None:intergenic 45.0%
TGTACCATTTCCATAGCTGC+TGG + chr2_2:58873324-58873343 None:intergenic 45.0%
GCTGCAAGAATGTTACTGTC+AGG - chr2_2:58873357-58873376 Msa0260900:intron 45.0%
AGTCCATCAGCACTTGTCAT+TGG + chr2_2:58873384-58873403 None:intergenic 45.0%
AAGGAAGGATTGCTCTTGTG+AGG + chr2_2:58873422-58873441 None:intergenic 45.0%
ACTGGTTAGGGGGGTTATTA+GGG + chr2_2:58873533-58873552 None:intergenic 45.0%
AACTGGTTAGGGGGGTTATT+AGG + chr2_2:58873534-58873553 None:intergenic 45.0%
GAAATTGGAACTGGTTAGGG+GGG + chr2_2:58873542-58873561 None:intergenic 45.0%
GGAAATTGGAACTGGTTAGG+GGG + chr2_2:58873543-58873562 None:intergenic 45.0%
GGGAAATTGGAACTGGTTAG+GGG + chr2_2:58873544-58873563 None:intergenic 45.0%
! CCCTCAAAATTTTCTGCCTC+AGG - chr2_2:58873566-58873585 Msa0260900:CDS 45.0%
GCTAAAGCTGCAGTGAAGTT+AGG + chr2_2:58873717-58873736 None:intergenic 45.0%
!! AAATTTATAAAGTAAAAATA+TGG + chr2_2:58870434-58870453 None:intergenic 5.0%
!!! TATTTTTACTTTATAAATTT+TGG - chr2_2:58870434-58870453 Msa0260900:intron 5.0%
AGGCAGATGCAAGGTTGTGA+TGG + chr2_2:58869973-58869992 None:intergenic 50.0%
TAGAGGTAGAGGCAGATGCA+AGG + chr2_2:58869982-58870001 None:intergenic 50.0%
AGCCGAGCTTGAGAGAATGA+AGG - chr2_2:58870774-58870793 Msa0260900:intron 50.0%
CGAGCTTGAGAGAATGAAGG+TGG - chr2_2:58870777-58870796 Msa0260900:intron 50.0%
GGTGCAAGACAAGAAGGTAG+AGG - chr2_2:58870876-58870895 Msa0260900:intron 50.0%
GCAGCTAAAACTCGATCTGC+AGG + chr2_2:58871162-58871181 None:intergenic 50.0%
AACAAGCTGAGGCCACTATG+AGG - chr2_2:58871232-58871251 Msa0260900:intron 50.0%
AGGCCACTATGAGGAAAGCA+AGG - chr2_2:58871241-58871260 Msa0260900:intron 50.0%
GGCCACTATGAGGAAAGCAA+GGG - chr2_2:58871242-58871261 Msa0260900:intron 50.0%
TTCAGTTAGAGCTCGATCCG+AGG - chr2_2:58871266-58871285 Msa0260900:intron 50.0%
TATCGATGCACCATGGCCTT+AGG - chr2_2:58871458-58871477 Msa0260900:intron 50.0%
! CCCACCCATAAGCTCTTTTC+AGG + chr2_2:58871958-58871977 None:intergenic 50.0%
! CAAGAAGTACGACCCGACTT+TGG + chr2_2:58872102-58872121 None:intergenic 50.0%
!! ACATTAGGGGGTGCTTGTAG+CGG - chr2_2:58872350-58872369 Msa0260900:CDS 50.0%
ACCATTTCCATAGCTGCTGG+AGG + chr2_2:58873321-58873340 None:intergenic 50.0%
!! GGACCAATGACAAGTGCTGA+TGG - chr2_2:58873378-58873397 Msa0260900:intron 50.0%
TAGGGGGGTTATTAGGGAAC+TGG + chr2_2:58873527-58873546 None:intergenic 50.0%
GAGGGAAGGGAAATTGGAAC+TGG + chr2_2:58873551-58873570 None:intergenic 50.0%
ACTGTGTCAGCAAGGTTCTG+TGG + chr2_2:58873675-58873694 None:intergenic 50.0%
! TGAGGGAGGTGTAGAGGTAG+AGG + chr2_2:58869993-58870012 None:intergenic 55.0%
AGCTCGATCCGAGGCTAACA+TGG - chr2_2:58871275-58871294 Msa0260900:intron 55.0%
CACAGGACAACCTAAGGCCA+TGG + chr2_2:58871471-58871490 None:intergenic 55.0%
AGGATGCGAGGGTGACATCA+TGG + chr2_2:58873126-58873145 None:intergenic 55.0%
TGATGTCACCCTCGCATCCT+AGG - chr2_2:58873126-58873145 Msa0260900:intron 55.0%
ATGGTTCACGACGGTGACCT+AGG + chr2_2:58873146-58873165 None:intergenic 55.0%
TGCTTTGCCTCCAGCAGCTA+TGG - chr2_2:58873311-58873330 Msa0260900:intron 55.0%
GCCTCCAGCAGCTATGGAAA+TGG - chr2_2:58873317-58873336 Msa0260900:intron 55.0%
!! AGGGGGTGCTTGTAGCGGAT+AGG - chr2_2:58872355-58872374 Msa0260900:CDS 60.0%
GACGGTGACCTAGGATGCGA+GGG + chr2_2:58873137-58873156 None:intergenic 60.0%
GGAGAGTCCAAGCCCTCCAC+TGG + chr2_2:58869711-58869730 None:intergenic 65.0%
CGACGGTGACCTAGGATGCG+AGG + chr2_2:58873138-58873157 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 58869704 58873902 58869704 ID=Msa0260900;Name=Msa0260900
chr2_2 mRNA 58869704 58873902 58869704 ID=Msa0260900-mRNA-1;Parent=Msa0260900;Name=Msa0260900-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.4|1|1|1|5|0|595
chr2_2 exon 58873519 58873902 58873519 ID=Msa0260900-mRNA-1:exon:18128;Parent=Msa0260900-mRNA-1
chr2_2 exon 58872950 58873048 58872950 ID=Msa0260900-mRNA-1:exon:18127;Parent=Msa0260900-mRNA-1
chr2_2 exon 58872310 58872846 58872310 ID=Msa0260900-mRNA-1:exon:18126;Parent=Msa0260900-mRNA-1
chr2_2 exon 58872107 58872220 58872107 ID=Msa0260900-mRNA-1:exon:18125;Parent=Msa0260900-mRNA-1
chr2_2 exon 58869704 58870357 58869704 ID=Msa0260900-mRNA-1:exon:18124;Parent=Msa0260900-mRNA-1
chr2_2 CDS 58873519 58873902 58873519 ID=Msa0260900-mRNA-1:cds;Parent=Msa0260900-mRNA-1
chr2_2 CDS 58872950 58873048 58872950 ID=Msa0260900-mRNA-1:cds;Parent=Msa0260900-mRNA-1
chr2_2 CDS 58872310 58872846 58872310 ID=Msa0260900-mRNA-1:cds;Parent=Msa0260900-mRNA-1
chr2_2 CDS 58872107 58872220 58872107 ID=Msa0260900-mRNA-1:cds;Parent=Msa0260900-mRNA-1
chr2_2 CDS 58869704 58870357 58869704 ID=Msa0260900-mRNA-1:cds;Parent=Msa0260900-mRNA-1
Gene Sequence

>Msa0260900

ATGGCCAGTGGAGGGCTTGGACTCTCCATTGATTCAGATCCAAATTCAAGCTTCTTATACCATAAGCAAATAGAATTCTCAACTCTTTTCCTCAACACAACAACCTCAAAAACTTCAAGTGGAAACTTATATAGTCACAAAATGGATACCACAAACAACAAAAACAAATCTCCATTTCAAGTCAACCATGAAATATCTCATCAACCCTTTGATCATGAAATGAGACCTATCATCAATGAACTAGACTTTTTCTCTCAAAACAACAACCATCACAACCTTGCATCTGCCTCTACCTCTACACCTCCCTCACTAAATCTTCATCATCATCATCATCATATACATGATCATTACACCGATCCTTCTCTCTTGGAATTCAAAGTCAACACTAGTTTGAATCTTCTTACGACCAACACAAGCAATGATCAATCCATGATGGAGGAAGACATAGCAAGTAATTCAGAAGACAAAAGAGCTAAGCTTGAGCTCGTTGTTCTTCAAGCCGAGCTTGAGAGAATGAAGGTGGAGAATCATCAACTGAGGAACATGCTTGATGAGGGTAATAGGAAGTACAACACATTGCAGATGCATTGGATGAGTATGGTGCAAGACAAGAAGGTAGAGGATTGTAATGAAGAGCAAAAGCAAGTGATTGGTGGAAAACTTGATGAGGAGAAACAAAATGGGAATGGTGGAGTTTTGGTTCCAAGACAATTTATGGAGCTTGGATTGGCTGCAAATAATTCTGATGCTATTGATGAACCAAGAAGTCAAGATCAGTCAAAATTACTTGCAAATAACAATGAGGAAGATTCCAAGGTTGAAGAACTTGTGTCTGATCATGAAAAGAAGGAATCCGATCGGGGAAAAGAAAGAAGCAGTAGTCCTGCAGATCGAGTTTTAGCTGCTAATAATACTAATAATAATGTTGCAAACTTTAGTCCTCAAACAAATGTTGAACAAGCTGAGGCCACTATGAGGAAAGCAAGGGTTTCAGTTAGAGCTCGATCCGAGGCTAACATGATCAATGATGGATGTCAATGGAGAAAGTATGGACAGAAGATGGCGAAAGGAAACCCGTGTCCTCGAGCTTATTATCGATGCACCATGGCCTTAGGTTGTCCTGTGAGAAAACAGGTACAAAGGTGTGCTGAAGACAAAACAATACTTATCACAACATATGAAGGACATCACATTCATGCTTTGCCTCCAGCAGCTATGGAAATGGTACAAACAACATCATCAGCTGCAAGAATGTTACTGTCAGGACCAATGACAAGTGCTGATGGACTAATGAACCCAAATTACCTCACAAGAGCAATCCTTCCTTATTCTTCAAGCATAGCAACTATCTCAGCATCAGCTCCATTTCCAACTGTTACATTGGACCTAACTCAGTCACCAAACCAAAACCAGTTCCCTAATAACCCCCCTAACCAGTTCCAATTTCCCTTCCCTCAAAATTTTCTGCCTCAGGTATTTGGTCAGACATTGTTGAACCAATCAAAATTTTCAGGACTTCAAATGTCTCAAGATGCAGCAAACAGTTCTCAGCAGACACCACAGAACCTTGCTGACACAGTTAATGCTATTGCAGCTGATCCTAACTTCACTGCAGCTTTAGCAGCTGCTATTACTTCAATTATTGGGGCTGCACAGCCAAACAATAATAACGGTACTAGCAATAATGGTAACAGTACAATAGCTAACAACACTAATGGCAATGTTACATCAAGTAACAATACCATTGGAAGCCCTAAAATCAACAATCCCAATTCCTCAGTAGAGTAG
Protein sequence

>Msa0260900

MASGGLGLSIDSDPNSSFLYHKQIEFSTLFLNTTTSKTSSGNLYSHKMDTTNNKNKSPFQVNHEISHQPFDHEMRPIINELDFFSQNNNHHNLASASTSTPPSLNLHHHHHHIHDHYTDPSLLEFKVNTSLNLLTTNTSNDQSMMEEDIASNSEDKRAKLELVVLQAELERMKVENHQLRNMLDEGNRKYNTLQMHWMSMVQDKKVEDCNEEQKQVIGGKLDEEKQNGNGGVLVPRQFMELGLAANNSDAIDEPRSQDQSKLLANNNEEDSKVEELVSDHEKKESDRGKERSSSPADRVLAANNTNNNVANFSPQTNVEQAEATMRKARVSVRARSEANMINDGCQWRKYGQKMAKGNPCPRAYYRCTMALGCPVRKQVQRCAEDKTILITTYEGHHIHALPPAAMEMVQTTSSAARMLLSGPMTSADGLMNPNYLTRAILPYSSSIATISASAPFPTVTLDLTQSPNQNQFPNNPPNQFQFPFPQNFLPQVFGQTLLNQSKFSGLQMSQDAANSSQQTPQNLADTVNAIAADPNFTAALAAAITSIIGAAQPNNNNGTSNNGNSTIANNTNGNVTSSNNTIGSPKINNPNSSVE*