AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0261110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261110 MtrunA17_Chr2g0322821 96.212 132 5 0 1 132 12 143 2.63e-89 255
Msa0261110 MtrunA17_Chr7g0267671 83.077 130 22 0 4 133 1 130 1.13e-76 223
Msa0261110 MtrunA17_Chr7g0267651 78.947 133 28 0 1 133 1 133 1.56e-73 215
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261110 AT5G62980.2 63.492 126 45 1 5 129 4 129 6.11e-52 160
Msa0261110 AT5G62980.1 63.492 126 45 1 5 129 4 129 6.11e-52 160
Msa0261110 AT3G11750.1 62.810 121 44 1 10 129 21 141 9.83e-51 158
Msa0261110 AT3G21730.1 52.500 120 56 1 11 129 40 159 5.30e-41 134
Msa0261110 AT3G21730.2 46.032 63 33 1 68 129 13 75 1.54e-13 62.0

Find 63 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGGGGATTGACTTTCTA+TGG 0.199149 2_2:-59235789 Msa0261110:CDS
GAAGGTGTTGATGCATTAAT+AGG 0.255291 2_2:-59235828 Msa0261110:CDS
AAAGCCTCATGTGGCAATTC+AGG 0.258560 2_2:-59235511 Msa0261110:CDS
ATTTGCTGTGATTTGTGTTT+GGG 0.279435 2_2:+59237025 None:intergenic
TATTTGCTGTGATTTGTGTT+TGG 0.293680 2_2:+59237024 None:intergenic
CGTCCACTGTCCAGTTTATC+TGG 0.312392 2_2:+59235279 None:intergenic
AACTTTACTCAAATACTCTT+TGG 0.331088 2_2:+59235206 None:intergenic
GGGGAGACAAACTTATTCTA+AGG 0.345136 2_2:-59235806 Msa0261110:CDS
CAAATTAAAATGTCAACTAT+TGG 0.352360 2_2:-59235121 Msa0261110:three_prime_UTR
GAAACTGTAACCAGATAAAC+TGG 0.360265 2_2:-59235289 Msa0261110:three_prime_UTR
AGGACCAGCTCAAAACCTTC+TGG 0.360872 2_2:-59235610 Msa0261110:CDS
TAAAATTGCAATCGCTACTT+TGG 0.362829 2_2:-59235574 Msa0261110:CDS
AGAGAGAAAGGTTGAGAAGA+TGG 0.380443 2_2:+59236998 None:intergenic
ATTTGATGTTCCTGTATGTA+TGG 0.407203 2_2:+59236966 None:intergenic
AAGGTGTTGATGCATTAATA+GGG 0.411585 2_2:-59235827 Msa0261110:CDS
CGGAGGTCTAATAAATGTTA+TGG 0.427674 2_2:-59234892 None:intergenic
AGTTTATACCTTGAAAAGCT+TGG 0.439205 2_2:+59236942 None:intergenic
GTTTATGTTTCTGTCCCTTA+CGG 0.439598 2_2:-59235045 Msa0261110:three_prime_UTR
AACTTTCATATTTACTATGC+AGG 0.446292 2_2:-59235429 Msa0261110:three_prime_UTR
ATCATATTGCGGTCTTGAAA+TGG 0.456318 2_2:-59234953 Msa0261110:three_prime_UTR
TGGTTGTAGAAGAGAGAGAA+AGG 0.458340 2_2:+59236986 None:intergenic
TGTTATTTCATTTCATATGC+CGG 0.462959 2_2:-59234912 Msa0261110:three_prime_UTR
ATAGCTAAGAAAGTTATTGA+AGG 0.467494 2_2:-59235630 Msa0261110:CDS
TTGTGCGTGGTAAGGCCGTA+AGG 0.479662 2_2:+59235030 None:intergenic
ATTAAGCTTTGTGCGTGGTA+AGG 0.492081 2_2:+59235022 None:intergenic
TCAACGCCTAAGTAATCAAC+CGG 0.493700 2_2:+59235486 None:intergenic
TCATGTGGCAATTCAGGGTC+CGG 0.499391 2_2:-59235505 Msa0261110:CDS
TCTCTATCTCATATAAGTCT+TGG 0.504545 2_2:-59235328 Msa0261110:three_prime_UTR
GAGATATCTAAAAGTAATGA+TGG 0.505822 2_2:+59235347 None:intergenic
CATAACATTTATTAGACCTC+CGG 0.505907 2_2:+59234893 None:intergenic
AAAGTAATGATGGTACGCTC+AGG 0.506318 2_2:+59235357 None:intergenic
TCTTGGTAAAATAATTAGAA+AGG 0.509972 2_2:-59235311 Msa0261110:three_prime_UTR
AAGCCTCATGTGGCAATTCA+GGG 0.514237 2_2:-59235510 Msa0261110:CDS
CAGGGTCCGGTTGATTACTT+AGG 0.521915 2_2:-59235492 Msa0261110:CDS
ACGGAGCAATAGCGGAAGAA+AGG 0.526769 2_2:-59235761 Msa0261110:CDS
TGGTTGGATCTCAAACCAGC+TGG 0.535894 2_2:-59235702 Msa0261110:CDS
GCGCGTGTGCATAGAATGGA+AGG 0.536353 2_2:-59235846 Msa0261110:intron
TCTTCTACAACCATACATAC+AGG 0.544158 2_2:-59236976 Msa0261110:five_prime_UTR
GGGAGACAAACTTATTCTAA+GGG 0.552621 2_2:-59235805 Msa0261110:CDS
GGTAGACGTGGATGCTTGGT+TGG 0.560167 2_2:-59235718 Msa0261110:CDS
GAGAAGATGAATTTCTGCTA+AGG 0.561918 2_2:+59237069 None:intergenic
AGGTGTTGATGCATTAATAG+GGG 0.577512 2_2:-59235826 Msa0261110:CDS
TTTGCTGTGATTTGTGTTTG+GGG 0.578421 2_2:+59237026 None:intergenic
TCTGCTGTTCGTGTGAAGGT+TGG 0.579288 2_2:-59235534 Msa0261110:CDS
TTGTGGTAGACGTGGATGCT+TGG 0.584289 2_2:-59235722 Msa0261110:CDS
TATGGGCCACTGACTCCAGA+AGG 0.586966 2_2:+59235595 None:intergenic
GACGCTAGGACAGAAGTTTG+TGG 0.591207 2_2:-59235739 Msa0261110:CDS
TCAAAACCTTCTGGAGTCAG+TGG 0.595009 2_2:-59235601 Msa0261110:CDS
AAGTAATGATGGTACGCTCA+GGG 0.598139 2_2:+59235358 None:intergenic
TGTGCGTGGTAAGGCCGTAA+GGG 0.619252 2_2:+59235031 None:intergenic
ATAGCGGAAGAAAGGACGCT+AGG 0.622684 2_2:-59235753 Msa0261110:CDS
GATATCTGCTGTTCGTGTGA+AGG 0.625108 2_2:-59235538 Msa0261110:CDS
AGAAACATAAACCTTCAAGC+TGG 0.627996 2_2:+59235056 None:intergenic
GGACCCTGAATTGCCACATG+AGG 0.645538 2_2:+59235507 None:intergenic
ATAGTTCGGTGATCATATTG+CGG 0.653255 2_2:-59234964 Msa0261110:three_prime_UTR
GGTGTTGATGCATTAATAGG+GGG 0.666097 2_2:-59235825 Msa0261110:CDS
TATTTCATTTCATATGCCGG+AGG 0.671792 2_2:-59234909 Msa0261110:three_prime_UTR
ACAGAAGTTTGTGGTAGACG+TGG 0.680810 2_2:-59235730 Msa0261110:CDS
AGCAGATTAAGCTTTGTGCG+TGG 0.682064 2_2:+59235017 None:intergenic
TAACCAGATAAACTGGACAG+TGG 0.698049 2_2:-59235282 Msa0261110:three_prime_UTR
GTTGAGATTCTGAGACGCAG+AGG 0.704169 2_2:-59235468 Msa0261110:CDS
GAAGGTTGGAAAGCCTCATG+TGG 0.704756 2_2:-59235520 Msa0261110:CDS
GGAGACAAACTTATTCTAAG+GGG 0.705478 2_2:-59235804 Msa0261110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTAATACTTTTTTATTTT+TGG + chr2_2:59235338-59235357 None:intergenic 10.0%
!!! AATTTCATTTTATATTATCT+TGG - chr2_2:59235713-59235732 Msa0261110:CDS 10.0%
!!! TCTTAATACTTTTTTATTTT+TGG + chr2_2:59235338-59235357 None:intergenic 10.0%
!!! AATTTCATTTTATATTATCT+TGG - chr2_2:59235713-59235732 Msa0261110:CDS 10.0%
!! ATACAATAATAAGAAATTAG+AGG + chr2_2:59235098-59235117 None:intergenic 15.0%
!!! TTATTTTCAGATTTTGATTT+TGG - chr2_2:59235117-59235136 Msa0261110:three_prime_UTR 15.0%
!!! TTAAGATTCATTTATTTCTT+AGG + chr2_2:59235601-59235620 None:intergenic 15.0%
!!! CTCATTTTTTTACTTTTAAT+CGG - chr2_2:59235842-59235861 Msa0261110:intron 15.0%
!! ATACAATAATAAGAAATTAG+AGG + chr2_2:59235098-59235117 None:intergenic 15.0%
!!! TTATTTTCAGATTTTGATTT+TGG - chr2_2:59235117-59235136 Msa0261110:three_prime_UTR 15.0%
!!! TTAAGATTCATTTATTTCTT+AGG + chr2_2:59235601-59235620 None:intergenic 15.0%
!!! CTCATTTTTTTACTTTTAAT+CGG - chr2_2:59235842-59235861 Msa0261110:intron 15.0%
!!! TTTTGGATTTAAGATTTCAT+AGG + chr2_2:59235321-59235340 None:intergenic 20.0%
!! ATCCAAAATCAAATTTACAA+AGG + chr2_2:59235414-59235433 None:intergenic 20.0%
!! TTTATGAAGTGATTTATATG+TGG - chr2_2:59235475-59235494 Msa0261110:CDS 20.0%
!! TCTTGGTAAAATAATTAGAA+AGG - chr2_2:59236668-59236687 Msa0261110:intron 20.0%
!!! CTGCATTTTTTTTTGTTATA+TGG + chr2_2:59236729-59236748 None:intergenic 20.0%
!!! CAATAGTTGACATTTTAATT+TGG + chr2_2:59236860-59236879 None:intergenic 20.0%
!! CAAATTAAAATGTCAACTAT+TGG - chr2_2:59236858-59236877 Msa0261110:intron 20.0%
!!! TTTTTTGGTATTTGATAGTT+CGG - chr2_2:59237001-59237020 Msa0261110:five_prime_UTR 20.0%
!!! TTTTGGATTTAAGATTTCAT+AGG + chr2_2:59235321-59235340 None:intergenic 20.0%
!! ATCCAAAATCAAATTTACAA+AGG + chr2_2:59235414-59235433 None:intergenic 20.0%
!! TTTATGAAGTGATTTATATG+TGG - chr2_2:59235475-59235494 Msa0261110:CDS 20.0%
!! TCTTGGTAAAATAATTAGAA+AGG - chr2_2:59236668-59236687 Msa0261110:intron 20.0%
!!! CTGCATTTTTTTTTGTTATA+TGG + chr2_2:59236729-59236748 None:intergenic 20.0%
!!! CAATAGTTGACATTTTAATT+TGG + chr2_2:59236860-59236879 None:intergenic 20.0%
!! CAAATTAAAATGTCAACTAT+TGG - chr2_2:59236858-59236877 Msa0261110:intron 20.0%
!!! TTTTTTGGTATTTGATAGTT+CGG - chr2_2:59237001-59237020 Msa0261110:five_prime_UTR 20.0%
!!! AGCCTTTGTAAATTTGATTT+TGG - chr2_2:59235409-59235428 Msa0261110:three_prime_UTR 25.0%
!! TTTTAATCGGACGTTTATTT+TGG - chr2_2:59235855-59235874 Msa0261110:intron 25.0%
! TTCTCATGATAACAAACAAA+GGG + chr2_2:59236086-59236105 None:intergenic 25.0%
! ATTCTCATGATAACAAACAA+AGG + chr2_2:59236087-59236106 None:intergenic 25.0%
! ATAGCTAAGAAAGTTATTGA+AGG - chr2_2:59236349-59236368 Msa0261110:intron 25.0%
! AACTTTCATATTTACTATGC+AGG - chr2_2:59236550-59236569 Msa0261110:intron 25.0%
! GAGATATCTAAAAGTAATGA+TGG + chr2_2:59236635-59236654 None:intergenic 25.0%
! AACTTTACTCAAATACTCTT+TGG + chr2_2:59236776-59236795 None:intergenic 25.0%
!!! AAGCAGTCCTTTTTTTTAAT+GGG + chr2_2:59236832-59236851 None:intergenic 25.0%
!!! AAAGCAGTCCTTTTTTTTAA+TGG + chr2_2:59236833-59236852 None:intergenic 25.0%
! ATTAAAAAAAAGGACTGCTT+TGG - chr2_2:59236832-59236851 Msa0261110:intron 25.0%
! GAACTATCAAATACCAAAAA+AGG + chr2_2:59237002-59237021 None:intergenic 25.0%
!!! TGTTATTTCATTTCATATGC+CGG - chr2_2:59237067-59237086 Msa0261110:five_prime_UTR 25.0%
!!! AGCCTTTGTAAATTTGATTT+TGG - chr2_2:59235409-59235428 Msa0261110:three_prime_UTR 25.0%
!! TTTTAATCGGACGTTTATTT+TGG - chr2_2:59235855-59235874 Msa0261110:intron 25.0%
! TTCTCATGATAACAAACAAA+GGG + chr2_2:59236086-59236105 None:intergenic 25.0%
! ATTCTCATGATAACAAACAA+AGG + chr2_2:59236087-59236106 None:intergenic 25.0%
! ATAGCTAAGAAAGTTATTGA+AGG - chr2_2:59236349-59236368 Msa0261110:intron 25.0%
! AACTTTCATATTTACTATGC+AGG - chr2_2:59236550-59236569 Msa0261110:intron 25.0%
! GAGATATCTAAAAGTAATGA+TGG + chr2_2:59236635-59236654 None:intergenic 25.0%
! AACTTTACTCAAATACTCTT+TGG + chr2_2:59236776-59236795 None:intergenic 25.0%
!!! AAGCAGTCCTTTTTTTTAAT+GGG + chr2_2:59236832-59236851 None:intergenic 25.0%
!!! AAAGCAGTCCTTTTTTTTAA+TGG + chr2_2:59236833-59236852 None:intergenic 25.0%
! ATTAAAAAAAAGGACTGCTT+TGG - chr2_2:59236832-59236851 Msa0261110:intron 25.0%
! GAACTATCAAATACCAAAAA+AGG + chr2_2:59237002-59237021 None:intergenic 25.0%
!!! TGTTATTTCATTTCATATGC+CGG - chr2_2:59237067-59237086 Msa0261110:five_prime_UTR 25.0%
!! ATTTGCTGTGATTTGTGTTT+GGG + chr2_2:59234957-59234976 None:intergenic 30.0%
!! TATTTGCTGTGATTTGTGTT+TGG + chr2_2:59234958-59234977 None:intergenic 30.0%
ATTTGATGTTCCTGTATGTA+TGG + chr2_2:59235016-59235035 None:intergenic 30.0%
! ATCAAATTCCAAGCTTTTCA+AGG - chr2_2:59235029-59235048 Msa0261110:three_prime_UTR 30.0%
AGTTTATACCTTGAAAAGCT+TGG + chr2_2:59235040-59235059 None:intergenic 30.0%
! GATAAGATCTAAGTTGAATC+AGG + chr2_2:59235153-59235172 None:intergenic 30.0%
! TTATCACTATGCTGTTTGTT+TGG - chr2_2:59235168-59235187 Msa0261110:three_prime_UTR 30.0%
! CTTTTGTCTCTTTCGTTTAT+TGG - chr2_2:59235219-59235238 Msa0261110:three_prime_UTR 30.0%
! TCAGTGGAGATTTTAAATCA+TGG - chr2_2:59235368-59235387 Msa0261110:three_prime_UTR 30.0%
!!! TGTTTTTTTAGTGCCTGTTT+GGG - chr2_2:59235678-59235697 Msa0261110:CDS 30.0%
! GTTTCGAGTGAAATGATTTA+TGG - chr2_2:59235764-59235783 Msa0261110:CDS 30.0%
! ACACTTTTATCGCAAAAAGT+AGG - chr2_2:59235791-59235810 Msa0261110:CDS 30.0%
TTGTGGTAAAAATCAAAGTG+TGG - chr2_2:59235815-59235834 Msa0261110:CDS 30.0%
TCTCATGATAACAAACAAAG+GGG + chr2_2:59236085-59236104 None:intergenic 30.0%
!! AAGGTGTTGATGCATTAATA+GGG - chr2_2:59236152-59236171 Msa0261110:intron 30.0%
! TTGACTTTCTATGGTTTTCA+CGG - chr2_2:59236199-59236218 Msa0261110:intron 30.0%
! AGTAGCGATTGCAATTTTAT+GGG + chr2_2:59236404-59236423 None:intergenic 30.0%
! AAGTAGCGATTGCAATTTTA+TGG + chr2_2:59236405-59236424 None:intergenic 30.0%
TAAAATTGCAATCGCTACTT+TGG - chr2_2:59236405-59236424 Msa0261110:intron 30.0%
TCTCTATCTCATATAAGTCT+TGG - chr2_2:59236651-59236670 Msa0261110:intron 30.0%
ACCACTTCCCATTAAAAAAA+AGG - chr2_2:59236822-59236841 Msa0261110:intron 30.0%
!!! TCCTTTTTTTTAATGGGAAG+TGG + chr2_2:59236826-59236845 None:intergenic 30.0%
!! ATTTGCTGTGATTTGTGTTT+GGG + chr2_2:59234957-59234976 None:intergenic 30.0%
!! TATTTGCTGTGATTTGTGTT+TGG + chr2_2:59234958-59234977 None:intergenic 30.0%
ATTTGATGTTCCTGTATGTA+TGG + chr2_2:59235016-59235035 None:intergenic 30.0%
! ATCAAATTCCAAGCTTTTCA+AGG - chr2_2:59235029-59235048 Msa0261110:three_prime_UTR 30.0%
AGTTTATACCTTGAAAAGCT+TGG + chr2_2:59235040-59235059 None:intergenic 30.0%
! GATAAGATCTAAGTTGAATC+AGG + chr2_2:59235153-59235172 None:intergenic 30.0%
! TTATCACTATGCTGTTTGTT+TGG - chr2_2:59235168-59235187 Msa0261110:three_prime_UTR 30.0%
! CTTTTGTCTCTTTCGTTTAT+TGG - chr2_2:59235219-59235238 Msa0261110:three_prime_UTR 30.0%
! TCAGTGGAGATTTTAAATCA+TGG - chr2_2:59235368-59235387 Msa0261110:three_prime_UTR 30.0%
!!! TGTTTTTTTAGTGCCTGTTT+GGG - chr2_2:59235678-59235697 Msa0261110:CDS 30.0%
! GTTTCGAGTGAAATGATTTA+TGG - chr2_2:59235764-59235783 Msa0261110:CDS 30.0%
! ACACTTTTATCGCAAAAAGT+AGG - chr2_2:59235791-59235810 Msa0261110:CDS 30.0%
TTGTGGTAAAAATCAAAGTG+TGG - chr2_2:59235815-59235834 Msa0261110:CDS 30.0%
TCTCATGATAACAAACAAAG+GGG + chr2_2:59236085-59236104 None:intergenic 30.0%
!! AAGGTGTTGATGCATTAATA+GGG - chr2_2:59236152-59236171 Msa0261110:intron 30.0%
! TTGACTTTCTATGGTTTTCA+CGG - chr2_2:59236199-59236218 Msa0261110:intron 30.0%
! AGTAGCGATTGCAATTTTAT+GGG + chr2_2:59236404-59236423 None:intergenic 30.0%
! AAGTAGCGATTGCAATTTTA+TGG + chr2_2:59236405-59236424 None:intergenic 30.0%
TAAAATTGCAATCGCTACTT+TGG - chr2_2:59236405-59236424 Msa0261110:intron 30.0%
TCTCTATCTCATATAAGTCT+TGG - chr2_2:59236651-59236670 Msa0261110:intron 30.0%
ACCACTTCCCATTAAAAAAA+AGG - chr2_2:59236822-59236841 Msa0261110:intron 30.0%
!!! TCCTTTTTTTTAATGGGAAG+TGG + chr2_2:59236826-59236845 None:intergenic 30.0%
GAGAAGATGAATTTCTGCTA+AGG + chr2_2:59234913-59234932 None:intergenic 35.0%
! TTTGCTGTGATTTGTGTTTG+GGG + chr2_2:59234956-59234975 None:intergenic 35.0%
TCTTCTACAACCATACATAC+AGG - chr2_2:59235003-59235022 Msa0261110:three_prime_UTR 35.0%
!!! GTGTTTTTTTAGTGCCTGTT+TGG - chr2_2:59235677-59235696 Msa0261110:CDS 35.0%
TATCGCAAAAAGTAGGTTTG+TGG - chr2_2:59235798-59235817 Msa0261110:CDS 35.0%
!! GAAGGTGTTGATGCATTAAT+AGG - chr2_2:59236151-59236170 Msa0261110:intron 35.0%
!! AGGTGTTGATGCATTAATAG+GGG - chr2_2:59236153-59236172 Msa0261110:intron 35.0%
GGGAGACAAACTTATTCTAA+GGG - chr2_2:59236174-59236193 Msa0261110:intron 35.0%
GGAGACAAACTTATTCTAAG+GGG - chr2_2:59236175-59236194 Msa0261110:intron 35.0%
! AAGTTATCAGATTTTCCAGC+TGG + chr2_2:59236295-59236314 None:intergenic 35.0%
GAAACTGTAACCAGATAAAC+TGG - chr2_2:59236690-59236709 Msa0261110:intron 35.0%
AGAAACATAAACCTTCAAGC+TGG + chr2_2:59236926-59236945 None:intergenic 35.0%
! GTTTATGTTTCTGTCCCTTA+CGG - chr2_2:59236934-59236953 Msa0261110:intron 35.0%
!!! GACAATTGACATGCCTTTTT+TGG - chr2_2:59236986-59237005 Msa0261110:five_prime_UTR 35.0%
! ATAGTTCGGTGATCATATTG+CGG - chr2_2:59237015-59237034 Msa0261110:five_prime_UTR 35.0%
ATCATATTGCGGTCTTGAAA+TGG - chr2_2:59237026-59237045 Msa0261110:five_prime_UTR 35.0%
TATTTCATTTCATATGCCGG+AGG - chr2_2:59237070-59237089 Msa0261110:five_prime_UTR 35.0%
GAGAAGATGAATTTCTGCTA+AGG + chr2_2:59234913-59234932 None:intergenic 35.0%
! TTTGCTGTGATTTGTGTTTG+GGG + chr2_2:59234956-59234975 None:intergenic 35.0%
TCTTCTACAACCATACATAC+AGG - chr2_2:59235003-59235022 Msa0261110:three_prime_UTR 35.0%
!!! GTGTTTTTTTAGTGCCTGTT+TGG - chr2_2:59235677-59235696 Msa0261110:CDS 35.0%
TATCGCAAAAAGTAGGTTTG+TGG - chr2_2:59235798-59235817 Msa0261110:CDS 35.0%
!! GAAGGTGTTGATGCATTAAT+AGG - chr2_2:59236151-59236170 Msa0261110:intron 35.0%
!! AGGTGTTGATGCATTAATAG+GGG - chr2_2:59236153-59236172 Msa0261110:intron 35.0%
GGGAGACAAACTTATTCTAA+GGG - chr2_2:59236174-59236193 Msa0261110:intron 35.0%
GGAGACAAACTTATTCTAAG+GGG - chr2_2:59236175-59236194 Msa0261110:intron 35.0%
! AAGTTATCAGATTTTCCAGC+TGG + chr2_2:59236295-59236314 None:intergenic 35.0%
GAAACTGTAACCAGATAAAC+TGG - chr2_2:59236690-59236709 Msa0261110:intron 35.0%
AGAAACATAAACCTTCAAGC+TGG + chr2_2:59236926-59236945 None:intergenic 35.0%
! GTTTATGTTTCTGTCCCTTA+CGG - chr2_2:59236934-59236953 Msa0261110:intron 35.0%
!!! GACAATTGACATGCCTTTTT+TGG - chr2_2:59236986-59237005 Msa0261110:five_prime_UTR 35.0%
! ATAGTTCGGTGATCATATTG+CGG - chr2_2:59237015-59237034 Msa0261110:five_prime_UTR 35.0%
ATCATATTGCGGTCTTGAAA+TGG - chr2_2:59237026-59237045 Msa0261110:five_prime_UTR 35.0%
TATTTCATTTCATATGCCGG+AGG - chr2_2:59237070-59237089 Msa0261110:five_prime_UTR 35.0%
AGAGAGAAAGGTTGAGAAGA+TGG + chr2_2:59234984-59235003 None:intergenic 40.0%
TGGTTGTAGAAGAGAGAGAA+AGG + chr2_2:59234996-59235015 None:intergenic 40.0%
TTAAGAGCTCAAAAGCTCAG+TGG - chr2_2:59235352-59235371 Msa0261110:three_prime_UTR 40.0%
TCAAGTTCTCCACTGAACTT+GGG + chr2_2:59235653-59235672 None:intergenic 40.0%
! TTCAAGTTCTCCACTGAACT+TGG + chr2_2:59235654-59235673 None:intergenic 40.0%
!!! CGACGTTTTGAAACAGAATC+AGG - chr2_2:59235916-59235935 Msa0261110:intron 40.0%
! TCAGGTTTTTCTTCAGCTGA+TGG - chr2_2:59235934-59235953 Msa0261110:intron 40.0%
! CAGGTTTTTCTTCAGCTGAT+GGG - chr2_2:59235935-59235954 Msa0261110:intron 40.0%
CAGCATCTGAAAAAGATCGA+AGG + chr2_2:59236044-59236063 None:intergenic 40.0%
!! GGTGTTGATGCATTAATAGG+GGG - chr2_2:59236154-59236173 Msa0261110:intron 40.0%
GGGGAGACAAACTTATTCTA+AGG - chr2_2:59236173-59236192 Msa0261110:intron 40.0%
! CTAAGGGGATTGACTTTCTA+TGG - chr2_2:59236190-59236209 Msa0261110:intron 40.0%
TCAACGCCTAAGTAATCAAC+CGG + chr2_2:59236496-59236515 None:intergenic 40.0%
AAGTAATGATGGTACGCTCA+GGG + chr2_2:59236624-59236643 None:intergenic 40.0%
AAAGTAATGATGGTACGCTC+AGG + chr2_2:59236625-59236644 None:intergenic 40.0%
TAACCAGATAAACTGGACAG+TGG - chr2_2:59236697-59236716 Msa0261110:intron 40.0%
!!! GAAGTTTTTTGCCAGCTTGA+AGG - chr2_2:59236912-59236931 Msa0261110:intron 40.0%
! ATTAAGCTTTGTGCGTGGTA+AGG + chr2_2:59236960-59236979 None:intergenic 40.0%
AGAGAGAAAGGTTGAGAAGA+TGG + chr2_2:59234984-59235003 None:intergenic 40.0%
TGGTTGTAGAAGAGAGAGAA+AGG + chr2_2:59234996-59235015 None:intergenic 40.0%
TTAAGAGCTCAAAAGCTCAG+TGG - chr2_2:59235352-59235371 Msa0261110:three_prime_UTR 40.0%
TCAAGTTCTCCACTGAACTT+GGG + chr2_2:59235653-59235672 None:intergenic 40.0%
! TTCAAGTTCTCCACTGAACT+TGG + chr2_2:59235654-59235673 None:intergenic 40.0%
!!! CGACGTTTTGAAACAGAATC+AGG - chr2_2:59235916-59235935 Msa0261110:intron 40.0%
! TCAGGTTTTTCTTCAGCTGA+TGG - chr2_2:59235934-59235953 Msa0261110:intron 40.0%
! CAGGTTTTTCTTCAGCTGAT+GGG - chr2_2:59235935-59235954 Msa0261110:intron 40.0%
CAGCATCTGAAAAAGATCGA+AGG + chr2_2:59236044-59236063 None:intergenic 40.0%
!! GGTGTTGATGCATTAATAGG+GGG - chr2_2:59236154-59236173 Msa0261110:intron 40.0%
GGGGAGACAAACTTATTCTA+AGG - chr2_2:59236173-59236192 Msa0261110:intron 40.0%
! CTAAGGGGATTGACTTTCTA+TGG - chr2_2:59236190-59236209 Msa0261110:intron 40.0%
TCAACGCCTAAGTAATCAAC+CGG + chr2_2:59236496-59236515 None:intergenic 40.0%
AAGTAATGATGGTACGCTCA+GGG + chr2_2:59236624-59236643 None:intergenic 40.0%
AAAGTAATGATGGTACGCTC+AGG + chr2_2:59236625-59236644 None:intergenic 40.0%
TAACCAGATAAACTGGACAG+TGG - chr2_2:59236697-59236716 Msa0261110:intron 40.0%
!!! GAAGTTTTTTGCCAGCTTGA+AGG - chr2_2:59236912-59236931 Msa0261110:intron 40.0%
! ATTAAGCTTTGTGCGTGGTA+AGG + chr2_2:59236960-59236979 None:intergenic 40.0%
! TGGTTTTCACGGAGCAATAG+CGG - chr2_2:59236210-59236229 Msa0261110:intron 45.0%
ACAGAAGTTTGTGGTAGACG+TGG - chr2_2:59236249-59236268 Msa0261110:intron 45.0%
!! TCAAAACCTTCTGGAGTCAG+TGG - chr2_2:59236378-59236397 Msa0261110:intron 45.0%
GATATCTGCTGTTCGTGTGA+AGG - chr2_2:59236441-59236460 Msa0261110:intron 45.0%
AAAGCCTCATGTGGCAATTC+AGG - chr2_2:59236468-59236487 Msa0261110:intron 45.0%
AAGCCTCATGTGGCAATTCA+GGG - chr2_2:59236469-59236488 Msa0261110:intron 45.0%
! AGCAGATTAAGCTTTGTGCG+TGG + chr2_2:59236965-59236984 None:intergenic 45.0%
! TGGTTTTCACGGAGCAATAG+CGG - chr2_2:59236210-59236229 Msa0261110:intron 45.0%
ACAGAAGTTTGTGGTAGACG+TGG - chr2_2:59236249-59236268 Msa0261110:intron 45.0%
!! TCAAAACCTTCTGGAGTCAG+TGG - chr2_2:59236378-59236397 Msa0261110:intron 45.0%
GATATCTGCTGTTCGTGTGA+AGG - chr2_2:59236441-59236460 Msa0261110:intron 45.0%
AAAGCCTCATGTGGCAATTC+AGG - chr2_2:59236468-59236487 Msa0261110:intron 45.0%
AAGCCTCATGTGGCAATTCA+GGG - chr2_2:59236469-59236488 Msa0261110:intron 45.0%
! AGCAGATTAAGCTTTGTGCG+TGG + chr2_2:59236965-59236984 None:intergenic 45.0%
TCAGTAGCTCCCAAGTTCAG+TGG - chr2_2:59235641-59235660 Msa0261110:CDS 50.0%
TTAGTGCCTGTTTGGGTGAG+TGG - chr2_2:59235685-59235704 Msa0261110:CDS 50.0%
TTGCAACCACTCACCCAAAC+AGG + chr2_2:59235694-59235713 None:intergenic 50.0%
! ACGGAGCAATAGCGGAAGAA+AGG - chr2_2:59236218-59236237 Msa0261110:intron 50.0%
! ATAGCGGAAGAAAGGACGCT+AGG - chr2_2:59236226-59236245 Msa0261110:intron 50.0%
GACGCTAGGACAGAAGTTTG+TGG - chr2_2:59236240-59236259 Msa0261110:intron 50.0%
TTGTGGTAGACGTGGATGCT+TGG - chr2_2:59236257-59236276 Msa0261110:intron 50.0%
TGGTTGGATCTCAAACCAGC+TGG - chr2_2:59236277-59236296 Msa0261110:intron 50.0%
AGGACCAGCTCAAAACCTTC+TGG - chr2_2:59236369-59236388 Msa0261110:intron 50.0%
!!! GACTCCAGAAGGTTTTGAGC+TGG + chr2_2:59236376-59236395 None:intergenic 50.0%
TCTGCTGTTCGTGTGAAGGT+TGG - chr2_2:59236445-59236464 Msa0261110:intron 50.0%
GAAGGTTGGAAAGCCTCATG+TGG - chr2_2:59236459-59236478 Msa0261110:intron 50.0%
TCATGTGGCAATTCAGGGTC+CGG - chr2_2:59236474-59236493 Msa0261110:intron 50.0%
CAGGGTCCGGTTGATTACTT+AGG - chr2_2:59236487-59236506 Msa0261110:intron 50.0%
!! GTTGAGATTCTGAGACGCAG+AGG - chr2_2:59236511-59236530 Msa0261110:intron 50.0%
CGTCCACTGTCCAGTTTATC+TGG + chr2_2:59236703-59236722 None:intergenic 50.0%
TCAGTAGCTCCCAAGTTCAG+TGG - chr2_2:59235641-59235660 Msa0261110:CDS 50.0%
TTAGTGCCTGTTTGGGTGAG+TGG - chr2_2:59235685-59235704 Msa0261110:CDS 50.0%
TTGCAACCACTCACCCAAAC+AGG + chr2_2:59235694-59235713 None:intergenic 50.0%
! ACGGAGCAATAGCGGAAGAA+AGG - chr2_2:59236218-59236237 Msa0261110:intron 50.0%
! ATAGCGGAAGAAAGGACGCT+AGG - chr2_2:59236226-59236245 Msa0261110:intron 50.0%
GACGCTAGGACAGAAGTTTG+TGG - chr2_2:59236240-59236259 Msa0261110:intron 50.0%
TTGTGGTAGACGTGGATGCT+TGG - chr2_2:59236257-59236276 Msa0261110:intron 50.0%
TGGTTGGATCTCAAACCAGC+TGG - chr2_2:59236277-59236296 Msa0261110:intron 50.0%
AGGACCAGCTCAAAACCTTC+TGG - chr2_2:59236369-59236388 Msa0261110:intron 50.0%
!!! GACTCCAGAAGGTTTTGAGC+TGG + chr2_2:59236376-59236395 None:intergenic 50.0%
TCTGCTGTTCGTGTGAAGGT+TGG - chr2_2:59236445-59236464 Msa0261110:intron 50.0%
GAAGGTTGGAAAGCCTCATG+TGG - chr2_2:59236459-59236478 Msa0261110:intron 50.0%
TCATGTGGCAATTCAGGGTC+CGG - chr2_2:59236474-59236493 Msa0261110:intron 50.0%
CAGGGTCCGGTTGATTACTT+AGG - chr2_2:59236487-59236506 Msa0261110:intron 50.0%
!! GTTGAGATTCTGAGACGCAG+AGG - chr2_2:59236511-59236530 Msa0261110:intron 50.0%
CGTCCACTGTCCAGTTTATC+TGG + chr2_2:59236703-59236722 None:intergenic 50.0%
GCGCGTGTGCATAGAATGGA+AGG - chr2_2:59236133-59236152 Msa0261110:intron 55.0%
GGTAGACGTGGATGCTTGGT+TGG - chr2_2:59236261-59236280 Msa0261110:intron 55.0%
!! TATGGGCCACTGACTCCAGA+AGG + chr2_2:59236387-59236406 None:intergenic 55.0%
GGACCCTGAATTGCCACATG+AGG + chr2_2:59236475-59236494 None:intergenic 55.0%
TGTGCGTGGTAAGGCCGTAA+GGG + chr2_2:59236951-59236970 None:intergenic 55.0%
TTGTGCGTGGTAAGGCCGTA+AGG + chr2_2:59236952-59236971 None:intergenic 55.0%
GCGCGTGTGCATAGAATGGA+AGG - chr2_2:59236133-59236152 Msa0261110:intron 55.0%
GGTAGACGTGGATGCTTGGT+TGG - chr2_2:59236261-59236280 Msa0261110:intron 55.0%
!! TATGGGCCACTGACTCCAGA+AGG + chr2_2:59236387-59236406 None:intergenic 55.0%
GGACCCTGAATTGCCACATG+AGG + chr2_2:59236475-59236494 None:intergenic 55.0%
TGTGCGTGGTAAGGCCGTAA+GGG + chr2_2:59236951-59236970 None:intergenic 55.0%
TTGTGCGTGGTAAGGCCGTA+AGG + chr2_2:59236952-59236971 None:intergenic 55.0%
GCGCGCGCGTGTGCATAGAA+TGG - chr2_2:59236129-59236148 Msa0261110:intron 65.0%
GCGCGCGCGTGTGCATAGAA+TGG - chr2_2:59236129-59236148 Msa0261110:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 59234894 59237107 59234894 ID=Msa0261110;Name=Msa0261110
chr2_2 mRNA 59234894 59237107 59234894 ID=Msa0261110-mRNA-1;Parent=Msa0261110;Name=Msa0261110-mRNA-1;_AED=0.33;_eAED=0.33;_QI=157|0|0.5|1|0|0.5|2|558|133
chr2_2 exon 59234894 59235853 59234894 ID=Msa0261110-mRNA-1:exon:18282;Parent=Msa0261110-mRNA-1
chr2_2 exon 59236951 59237107 59236951 ID=Msa0261110-mRNA-1:exon:18281;Parent=Msa0261110-mRNA-1
chr2_2 five_prime_UTR 59236951 59237107 59236951 ID=Msa0261110-mRNA-1:five_prime_utr;Parent=Msa0261110-mRNA-1
chr2_2 CDS 59235452 59235853 59235452 ID=Msa0261110-mRNA-1:cds;Parent=Msa0261110-mRNA-1
chr2_2 three_prime_UTR 59234894 59235451 59234894 ID=Msa0261110-mRNA-1:three_prime_utr;Parent=Msa0261110-mRNA-1
Gene Sequence

>Msa0261110

ATGGAAGGTGTTGATGCATTAATAGGGGGAGACAAACTTATTCTAAGGGGATTGACTTTCTATGGTTTTCACGGAGCAATAGCGGAAGAAAGGACGCTAGGACAGAAGTTTGTGGTAGACGTGGATGCTTGGTTGGATCTCAAACCAGCTGGAAAATCTGATAACTTGTCTGATTCTTTTAGTTACGCTGAAATATATCTTATAGCTAAGAAAGTTATTGAAGGACCAGCTCAAAACCTTCTGGAGTCAGTGGCCCATAAAATTGCAATCGCTACTTTGGAAGTTCAGAAAAAGATATCTGCTGTTCGTGTGAAGGTTGGAAAGCCTCATGTGGCAATTCAGGGTCCGGTTGATTACTTAGGCGTTGAGATTCTGAGACGCAGAGGCGATTTGACAGACTAG
Protein sequence

>Msa0261110

MEGVDALIGGDKLILRGLTFYGFHGAIAEERTLGQKFVVDVDAWLDLKPAGKSDNLSDSFSYAEIYLIAKKVIEGPAQNLLESVAHKIAIATLEVQKKISAVRVKVGKPHVAIQGPVDYLGVEILRRRGDLTD*