AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0261130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261130 MtrunA17_Chr2g0323131 92.945 567 40 0 3 569 1 567 0.0 1102
Msa0261130 MtrunA17_Chr2g0323151 85.000 480 71 1 87 566 1 479 0.0 872
Msa0261130 MtrunA17_Chr4g0023911 40.283 494 275 8 64 546 74 558 2.36e-118 362
Msa0261130 MtrunA17_Chr4g0042111 37.177 503 299 7 57 546 41 539 4.89e-111 342
Msa0261130 MtrunA17_Chr2g0310331 36.121 562 347 6 15 566 26 585 1.23e-110 342
Msa0261130 MtrunA17_Chr4g0048911 33.631 559 343 11 21 561 6 554 6.39e-106 330
Msa0261130 MtrunA17_Chr4g0048921 35.882 510 313 7 62 562 62 566 1.31e-101 318
Msa0261130 MtrunA17_Chr6g0451711 35.271 499 301 8 83 566 65 556 2.98e-100 314
Msa0261130 MtrunA17_Chr4g0024691 34.774 532 320 11 49 566 42 560 5.27e-99 311
Msa0261130 MtrunA17_Chr2g0310401 40.187 428 246 5 145 566 8 431 1.02e-98 306
Msa0261130 MtrunA17_Chr8g0357411 34.848 528 331 7 50 566 63 588 1.46e-97 308
Msa0261130 MtrunA17_Chr6g0465441 32.941 510 327 8 69 566 46 552 1.68e-97 307
Msa0261130 MtrunA17_Chr7g0236961 35.021 474 290 6 86 547 9 476 7.89e-97 303
Msa0261130 MtrunA17_Chr7g0237011 35.021 474 290 6 86 547 9 476 1.43e-96 303
Msa0261130 MtrunA17_Chr7g0236991 34.350 492 304 7 69 547 46 531 2.41e-96 304
Msa0261130 MtrunA17_Chr7g0237001 34.350 492 304 7 69 547 46 531 4.37e-96 303
Msa0261130 MtrunA17_Chr7g0237041 34.350 492 304 7 69 547 46 531 4.37e-96 303
Msa0261130 MtrunA17_Chr7g0237081 34.350 492 304 7 69 547 46 531 4.37e-96 303
Msa0261130 MtrunA17_Chr7g0237051 34.350 492 304 7 69 547 46 531 4.37e-96 303
Msa0261130 MtrunA17_Chr2g0280581 33.202 506 325 4 69 561 12 517 6.63e-95 299
Msa0261130 MtrunA17_Chr5g0425371 34.118 510 315 8 72 566 49 552 2.13e-94 299
Msa0261130 MtrunA17_Chr3g0099031 33.333 501 320 6 74 561 13 512 2.86e-94 298
Msa0261130 MtrunA17_Chr3g0109001 33.730 504 315 5 74 561 13 513 4.52e-94 297
Msa0261130 MtrunA17_Chr3g0108941 34.185 509 314 7 74 565 13 517 1.19e-93 296
Msa0261130 MtrunA17_Chr3g0105071 35.000 480 289 7 83 547 64 535 6.42e-93 295
Msa0261130 MtrunA17_Chr6g0477111 34.774 486 297 7 92 561 6 487 5.25e-92 291
Msa0261130 MtrunA17_Chr3g0108991 34.122 507 316 9 74 565 13 516 5.78e-92 291
Msa0261130 MtrunA17_Chr3g0108951 34.122 507 316 9 74 565 13 516 5.78e-92 291
Msa0261130 MtrunA17_Chr3g0108981 33.538 489 306 5 89 561 3 488 6.82e-92 291
Msa0261130 MtrunA17_Chr3g0108961 33.538 489 306 5 89 561 3 488 6.82e-92 291
Msa0261130 MtrunA17_Chr3g0082691 33.466 502 309 8 82 566 59 552 8.59e-92 292
Msa0261130 MtrunA17_Chr3g0109021 33.598 503 316 9 74 561 13 512 1.12e-91 291
Msa0261130 MtrunA17_Chr2g0319051 34.524 504 311 9 73 561 9 508 1.72e-91 290
Msa0261130 MtrunA17_Chr3g0108971 34.756 492 303 9 89 565 3 491 2.26e-91 289
Msa0261130 MtrunA17_Chr6g0465851 32.617 512 326 7 69 566 11 517 1.75e-90 288
Msa0261130 MtrunA17_Chr3g0109031 31.881 505 330 5 74 565 13 516 1.94e-90 288
Msa0261130 MtrunA17_Chr6g0465911 32.617 512 326 7 69 566 46 552 3.89e-90 288
Msa0261130 MtrunA17_Chr6g0466051 32.617 512 326 7 69 566 46 552 3.89e-90 288
Msa0261130 MtrunA17_Chr3g0105691 34.146 492 310 7 86 564 58 548 4.54e-90 288
Msa0261130 MtrunA17_Chr5g0431651 33.663 505 304 9 82 566 58 551 4.21e-89 285
Msa0261130 MtrunA17_Chr2g0319041 32.567 522 328 11 59 561 35 551 6.87e-89 285
Msa0261130 MtrunA17_Chr3g0109011 33.129 489 307 7 89 561 3 487 2.75e-88 281
Msa0261130 MtrunA17_Chr5g0431571 34.888 493 295 9 72 547 44 527 1.54e-86 278
Msa0261130 MtrunA17_Chr8g0336181 33.529 510 307 11 62 550 39 537 7.85e-85 274
Msa0261130 MtrunA17_Chr6g0477121 36.058 416 252 8 143 549 8 418 3.17e-81 261
Msa0261130 MtrunA17_Chr7g0236951 40.514 311 172 2 86 386 9 316 4.87e-77 246
Msa0261130 MtrunA17_Chr7g0237031 40.514 311 172 2 86 386 9 316 8.74e-77 246
Msa0261130 MtrunA17_Chr7g0236971 34.375 384 247 4 168 549 1 381 8.71e-76 246
Msa0261130 MtrunA17_Chr6g0466111 39.394 330 186 3 69 387 11 337 2.16e-73 238
Msa0261130 MtrunA17_Chr6g0465981 39.394 330 186 3 69 387 46 372 3.99e-73 238
Msa0261130 MtrunA17_Chr6g0465871 39.394 330 186 3 69 387 46 372 3.99e-73 238
Msa0261130 MtrunA17_Chr6g0466081 39.394 330 186 3 69 387 46 372 3.99e-73 238
Msa0261130 MtrunA17_Chr6g0465931 40.584 308 170 2 90 387 33 337 2.94e-72 234
Msa0261130 MtrunA17_Chr4g0008621 35.674 356 217 4 84 428 70 424 3.83e-66 221
Msa0261130 MtrunA17_Chr7g0217361 39.427 279 165 4 270 546 11 287 6.97e-62 206
Msa0261130 MtrunA17_Chr5g0445511 40.756 238 141 0 164 401 2 239 4.90e-55 186
Msa0261130 MtrunA17_Chr2g0319071 27.642 492 294 12 90 567 46 489 3.49e-49 177
Msa0261130 MtrunA17_Chr8g0357231 32.692 260 170 4 310 566 1 258 2.20e-47 166
Msa0261130 MtrunA17_Chr2g0310121 25.911 494 330 12 85 546 271 760 2.56e-39 153
Msa0261130 MtrunA17_Chr2g0319081 31.208 298 183 9 270 561 140 421 2.27e-37 143
Msa0261130 MtrunA17_Chr7g0237071 38.614 202 108 3 86 274 9 207 1.46e-36 135
Msa0261130 MtrunA17_Chr3g0101681 25.050 503 338 13 90 560 277 772 2.59e-36 144
Msa0261130 MtrunA17_Chr6g0466031 26.154 260 187 4 310 566 2 259 5.44e-29 115
Msa0261130 MtrunA17_Chr7g0237061 28.216 241 147 6 310 549 2 217 9.38e-28 111
Msa0261130 MtrunA17_Chr2g0281651 26.415 318 220 7 245 555 43 353 2.27e-27 114
Msa0261130 MtrunA17_Chr8g0357221 34.081 223 139 3 50 264 63 285 3.87e-27 111
Msa0261130 MtrunA17_Chr7g0233861 40.650 123 64 1 220 342 45 158 6.36e-23 96.3
Msa0261130 MtrunA17_Chr6g0466021 37.419 155 83 3 72 215 49 200 3.51e-22 94.7
Msa0261130 MtrunA17_Chr7g0259881 29.167 288 160 11 92 340 232 514 4.35e-20 94.4
Msa0261130 MtrunA17_Chr7g0217351 31.163 215 131 6 66 270 66 273 5.02e-19 87.8
Msa0261130 MtrunA17_Chr7g0218001 28.352 261 150 7 92 317 349 607 9.53e-17 84.0
Msa0261130 MtrunA17_Chr2g0281721 35.000 100 63 2 245 343 29 127 1.12e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261130 AT4G16730.1 40.856 514 281 7 73 566 28 538 8.01e-134 400
Msa0261130 AT4G16730.2 41.414 495 267 7 92 566 1 492 1.09e-132 396
Msa0261130 AT4G16740.1 37.214 524 298 8 63 566 51 563 6.66e-112 345
Msa0261130 AT2G24210.1 35.758 495 296 9 90 566 99 589 2.52e-106 332
Msa0261130 AT3G25820.1 35.305 524 303 11 65 566 84 593 3.71e-105 329
Msa0261130 AT3G25830.1 35.305 524 303 11 65 566 84 593 3.71e-105 329
Msa0261130 AT1G61680.1 36.345 487 300 6 82 561 86 569 9.00e-104 324
Msa0261130 AT3G25810.1 35.000 500 298 10 90 566 101 596 6.72e-102 320
Msa0261130 AT1G61680.2 35.729 487 296 7 82 561 86 562 1.41e-99 313
Msa0261130 AT5G23960.2 30.962 520 346 7 58 566 27 544 1.51e-85 276
Msa0261130 AT5G23960.1 31.034 522 345 8 58 566 27 546 2.13e-84 273
Msa0261130 AT1G70080.1 32.653 490 302 11 92 560 122 604 2.34e-79 261
Msa0261130 AT4G16740.2 38.484 343 188 5 63 388 51 387 6.13e-73 238
Msa0261130 AT3G25820.2 36.695 357 198 8 65 406 84 427 7.89e-72 237
Msa0261130 AT3G14490.1 30.769 507 331 10 72 565 95 594 2.22e-68 232
Msa0261130 AT3G29410.2 30.258 466 313 6 90 546 13 475 3.08e-66 224
Msa0261130 AT3G29410.1 30.258 466 313 6 90 546 118 580 3.02e-65 224
Msa0261130 AT1G33750.1 29.198 524 352 9 54 566 87 602 3.68e-65 224
Msa0261130 AT3G14540.1 30.316 475 310 8 90 549 113 581 1.47e-62 216
Msa0261130 AT1G31950.3 28.330 533 359 9 50 566 81 606 2.59e-62 216
Msa0261130 AT1G31950.1 29.323 532 354 11 50 566 81 605 2.62e-62 216
Msa0261130 AT4G15870.1 28.015 539 355 13 20 547 60 576 8.34e-61 212
Msa0261130 AT3G14520.1 27.789 493 335 8 90 566 116 603 3.57e-58 205
Msa0261130 AT5G44630.1 30.252 476 305 11 87 546 70 534 1.08e-57 202
Msa0261130 AT4G16740.4 36.478 318 179 5 63 363 51 362 1.72e-55 192
Msa0261130 AT4G16740.3 36.478 318 179 5 63 363 51 362 1.72e-55 192
Msa0261130 AT3G32030.1 26.419 511 357 7 72 567 98 604 3.72e-55 196
Msa0261130 AT4G13300.1 28.571 511 348 9 66 561 40 548 1.17e-54 194
Msa0261130 AT2G23230.2 29.848 526 324 14 66 561 84 594 1.93e-53 192
Msa0261130 AT4G20230.2 28.200 461 319 7 92 542 131 589 3.10e-53 191
Msa0261130 AT4G20230.1 28.200 461 319 7 92 542 119 577 3.12e-53 191
Msa0261130 AT3G25820.4 36.860 293 157 8 65 342 84 363 3.56e-53 186
Msa0261130 AT3G25820.3 36.860 293 157 8 65 342 84 363 3.56e-53 186
Msa0261130 AT2G23230.1 29.942 521 325 14 66 561 84 589 5.50e-53 191
Msa0261130 AT1G31950.2 29.630 432 288 7 50 470 81 507 1.03e-52 188
Msa0261130 AT3G29110.1 28.968 504 321 11 67 546 84 574 1.80e-52 189
Msa0261130 AT3G14540.2 30.886 395 253 7 90 470 113 501 4.09e-52 186
Msa0261130 AT4G13280.2 28.924 446 308 6 123 561 105 548 4.78e-52 187
Msa0261130 AT3G29190.1 29.710 414 281 6 92 496 116 528 1.80e-51 186
Msa0261130 AT5G48110.1 29.688 320 220 4 228 546 2 317 2.22e-51 180
Msa0261130 AT4G20210.1 28.081 495 324 11 92 565 115 598 1.17e-50 184
Msa0261130 AT1G66020.1 29.247 465 309 9 92 542 113 571 4.64e-50 182
Msa0261130 AT4G20200.1 29.274 468 305 8 92 542 119 577 4.93e-50 182
Msa0261130 AT1G48800.1 29.645 479 311 13 90 552 118 586 5.26e-50 182
Msa0261130 AT1G48820.1 26.994 489 311 12 67 547 92 542 7.89e-44 164
Msa0261130 AT1G66020.2 36.071 280 162 6 92 358 113 388 1.39e-43 161
Msa0261130 AT4G13280.1 27.578 446 284 8 123 561 105 518 1.70e-42 160
Msa0261130 AT1G79460.1 25.341 513 340 13 85 562 270 774 1.37e-33 136
Msa0261130 AT1G61120.1 26.478 423 273 7 84 478 280 692 2.49e-31 130
Msa0261130 AT4G02780.1 30.916 262 142 10 92 317 334 592 1.27e-18 90.5

Find 120 sgRNAs with CRISPR-Local

Find 305 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTTGCTCGTTGTAATTT+TGG 0.081136 2_2:+59295908 Msa0261130:CDS
CGGTTAGTCCATGTTCTTTC+TGG 0.088351 2_2:-59300186 None:intergenic
TCCAAATATGTCTTAAAATT+TGG 0.090930 2_2:-59300386 None:intergenic
TTATATGAGGCATCATATTT+AGG 0.175329 2_2:+59298972 Msa0261130:CDS
ATTCCAAAAGAGTAGGTATT+TGG 0.197928 2_2:-59299181 None:intergenic
CCGGGGAAATTCTTCGACTA+TGG 0.229539 2_2:+59300534 Msa0261130:CDS
TAACCAAATACCTACTCTTT+TGG 0.241157 2_2:+59299178 Msa0261130:CDS
TTTAAGACATATTTGGATAA+TGG 0.251513 2_2:+59300392 Msa0261130:CDS
TATCAAAGCTTCTCTTAATA+TGG 0.262436 2_2:+59301365 Msa0261130:CDS
TATATGAGGCATCATATTTA+GGG 0.283045 2_2:+59298973 Msa0261130:CDS
ATATTTCTGCCAATTCTCTT+TGG 0.285413 2_2:-59299241 None:intergenic
TGAAGATAAGACCAAAAGTT+TGG 0.285905 2_2:+59297365 Msa0261130:CDS
GCCATTGTCATTGTATTTGC+AGG 0.288475 2_2:-59295851 None:intergenic
CAATTTGAATGACGTGGTTC+TGG 0.288793 2_2:-59299863 None:intergenic
ATCACTTACCAATGACATTT+GGG 0.292181 2_2:+59298938 Msa0261130:CDS
GATGATATCTTTGACACTTA+TGG 0.293561 2_2:+59299929 Msa0261130:CDS
TTTGACTTGACCCAAACTTT+TGG 0.296123 2_2:-59297376 None:intergenic
CCAATTTGCCTGCCTACTTC+AGG 0.296197 2_2:-59299074 None:intergenic
CATTTCTTGAAGAAGCTAAA+TGG 0.310663 2_2:+59300345 Msa0261130:CDS
CTTCTAACCTTGCCATTCTT+AGG 0.311069 2_2:-59299121 None:intergenic
TTGCCACATCCCTTCAATTT+CGG 0.335451 2_2:+59297556 Msa0261130:CDS
ATACATGAAAATATGTTATA+TGG 0.345162 2_2:+59300126 Msa0261130:CDS
CTTGCCATTCTTAGGTGCTT+TGG 0.346897 2_2:-59299113 None:intergenic
AATCACTTACCAATGACATT+TGG 0.351614 2_2:+59298937 Msa0261130:CDS
CCTGAAGTAGGCAGGCAAAT+TGG 0.353627 2_2:+59299074 Msa0261130:CDS
ATGAAGGAAGAATACAAATA+AGG 0.356949 2_2:+59301274 Msa0261130:CDS
CCCAGGCTCTTCACTTGCTC+CGG 0.372004 2_2:+59300515 Msa0261130:CDS
GCTGGGGATTGGTCACCATT+TGG 0.377069 2_2:+59297473 Msa0261130:CDS
GACCAACGGAGTGCTTCTTA+TGG 0.384882 2_2:+59299825 Msa0261130:CDS
ATTAGGTGGTGGAAAGAGTT+GGG 0.386750 2_2:+59299770 Msa0261130:intron
GAAGCTAAATGGTTCAATAA+TGG 0.388219 2_2:+59300356 Msa0261130:CDS
GTCATTACACCAAAGAGAAT+TGG 0.404125 2_2:+59299232 Msa0261130:CDS
TTGAATTTAGCAATGCTTCT+CGG 0.408026 2_2:-59297407 None:intergenic
TTTCGGTTACTGAGGCATAA+TGG 0.414627 2_2:+59297573 Msa0261130:CDS
AACTTGGGAGGATATGTGAT+TGG 0.414855 2_2:+59297514 Msa0261130:CDS
ATACAAATAAGGATGCTACT+AGG 0.418565 2_2:+59301285 Msa0261130:CDS
CCATTCTTAGGTGCTTTGGA+AGG 0.418608 2_2:-59299109 None:intergenic
TCTATATGCAATTTCATTTG+TGG 0.420807 2_2:-59300161 None:intergenic
GGGTATGTTGAGTTTATATG+AGG 0.434234 2_2:+59298959 Msa0261130:CDS
ATCATCTTCAATGTTACAAT+AGG 0.441194 2_2:-59297435 None:intergenic
GGATGCTACTAGGGAATTTG+TGG 0.444878 2_2:+59301295 Msa0261130:CDS
GAAGATAAGACCAAAAGTTT+GGG 0.446624 2_2:+59297366 Msa0261130:CDS
ATTCTCTTTGGTGTAATGAC+TGG 0.447027 2_2:-59299229 None:intergenic
ATGCATGCCACCTTCCTCAT+TGG 0.449603 2_2:+59300446 Msa0261130:CDS
TCAATCCAAGGGCTGGGGAT+TGG 0.453647 2_2:+59297462 Msa0261130:CDS
TAGGTGCTTTGGAAGGGTTA+AGG 0.456059 2_2:-59299102 None:intergenic
GCCTGCCTACTTCAGGACTA+AGG 0.459721 2_2:-59299067 None:intergenic
CAACATACCCCAAATGTCAT+TGG 0.463701 2_2:-59298946 None:intergenic
CATAACTAGCAAAATACATA+TGG 0.467636 2_2:-59299906 None:intergenic
CCAGGCTCTTCACTTGCTCC+GGG 0.468250 2_2:+59300516 Msa0261130:CDS
GATAATGGAGTGATTTCTGC+AGG 0.473286 2_2:+59300407 Msa0261130:CDS
ATTGACTCAATCCAAGGGCT+GGG 0.473312 2_2:+59297456 Msa0261130:CDS
TTGGGACTTGTTGACAAACT+TGG 0.473750 2_2:+59299788 Msa0261130:CDS
GATTGACTCAATCCAAGGGC+TGG 0.474173 2_2:+59297455 Msa0261130:CDS
GTCTTATCTTCAGTTTGTAA+AGG 0.481471 2_2:-59297354 None:intergenic
GATTAGGTGGTGGAAAGAGT+TGG 0.483866 2_2:+59299769 Msa0261130:intron
TACAAATAAGGATGCTACTA+GGG 0.488175 2_2:+59301286 Msa0261130:CDS
CTTCGACTATGGGATGACTT+AGG 0.488396 2_2:+59300545 Msa0261130:CDS
CGGGGAAATTCTTCGACTAT+GGG 0.489879 2_2:+59300535 Msa0261130:CDS
AATGGCTGGCCCACATGTCC+TGG 0.494407 2_2:+59297591 Msa0261130:CDS
CATTGATCTCATCTTCCAAA+TGG 0.495809 2_2:-59297488 None:intergenic
ACTTGGGAGGATATGTGATT+GGG 0.499523 2_2:+59297515 Msa0261130:CDS
GGTGACCAATCCCCAGCCCT+TGG 0.500245 2_2:-59297467 None:intergenic
GCCACCTTCCTCATTGGTGA+TGG 0.504456 2_2:+59300452 Msa0261130:CDS
CATTCTTAGGTGCTTTGGAA+GGG 0.508082 2_2:-59299108 None:intergenic
GCCTCAGTAACCGAAATTGA+AGG 0.515765 2_2:-59297566 None:intergenic
AATATTTCAGATGAAAATGA+AGG 0.518222 2_2:+59301258 Msa0261130:CDS
GATTTCTGCAGGATCATGTA+TGG 0.522815 2_2:+59300418 Msa0261130:CDS
GAGTGCTTCTTATGGACAGT+AGG 0.523662 2_2:+59299833 Msa0261130:CDS
CCTTCCAAAGCACCTAAGAA+TGG 0.525082 2_2:+59299109 Msa0261130:CDS
GGGATATGACTTCATCATCA+TGG 0.527240 2_2:-59300495 None:intergenic
ACCTTAGTCCTGAAGTAGGC+AGG 0.530056 2_2:+59299066 Msa0261130:CDS
GGTTACTGAGGCATAATGGC+TGG 0.530808 2_2:+59297577 Msa0261130:CDS
GAGAATTGGCAGAAATATGC+AGG 0.535303 2_2:+59299246 Msa0261130:CDS
CAGATGGGACCTTGATGCAA+TGG 0.543941 2_2:+59300090 Msa0261130:intron
GAAGTTGTTCCATTGCATCA+AGG 0.549004 2_2:-59300099 None:intergenic
CCCTTCAATTTCGGTTACTG+AGG 0.554070 2_2:+59297565 Msa0261130:CDS
ACGAGCCCTTGAAAATTCCA+TGG 0.555738 2_2:-59299024 None:intergenic
GTTCAGGAGGAACAAGAAAG+AGG 0.555777 2_2:+59301195 Msa0261130:intron
CCCGGAGCAAGTGAAGAGCC+TGG 0.558035 2_2:-59300516 None:intergenic
TAACCGAAATTGAAGGGATG+TGG 0.558085 2_2:-59297559 None:intergenic
AGGTAATGATGTGAAGATCA+AGG 0.564747 2_2:-59301460 None:intergenic
TTTGCCAATTCCAAAAGAGT+AGG 0.571866 2_2:-59299188 None:intergenic
GATGAGATCAATGTTCAACT+TGG 0.572324 2_2:+59297498 Msa0261130:CDS
AAGATGATTGACTCAATCCA+AGG 0.578634 2_2:+59297450 Msa0261130:CDS
GTCCATTGCTCTCAATGAGA+AGG 0.579260 2_2:+59296009 Msa0261130:CDS
CCACACCTTAGTCCTGAAGT+AGG 0.581171 2_2:+59299062 Msa0261130:CDS
CCTCAGTAACCGAAATTGAA+GGG 0.583119 2_2:-59297565 None:intergenic
CCGGAGCAAGTGAAGAGCCT+GGG 0.583946 2_2:-59300515 None:intergenic
ATGCAATATACCAGGACATG+TGG 0.584364 2_2:-59297601 None:intergenic
TGATGATGAAGTCATATCCC+AGG 0.589646 2_2:+59300498 Msa0261130:CDS
CTACTTCAGGACTAAGGTGT+GGG 0.590174 2_2:-59299061 None:intergenic
TACCTTCTCATTGAGAGCAA+TGG 0.593656 2_2:-59296011 None:intergenic
CATTGGTGATGGTCTCTCAA+AGG 0.594573 2_2:+59300463 Msa0261130:CDS
ATGAGATCAATGTTCAACTT+GGG 0.595916 2_2:+59297499 Msa0261130:CDS
TGCAATATACCAGGACATGT+GGG 0.599170 2_2:-59297600 None:intergenic
TATAGAATCCAGAAAGAACA+TGG 0.603675 2_2:+59300178 Msa0261130:CDS
CCATAGTCGAAGAATTTCCC+CGG 0.608178 2_2:-59300534 None:intergenic
GTCCATAAGAAGCACTCCGT+TGG 0.609668 2_2:-59299827 None:intergenic
TTGACTCAATCCAAGGGCTG+GGG 0.610758 2_2:+59297457 Msa0261130:CDS
ATGCGGCAATTTGAATGACG+TGG 0.612524 2_2:-59299869 None:intergenic
TCAAATTCAAGGATTATGCA+TGG 0.614482 2_2:+59295971 Msa0261130:CDS
AGTGCTTCTTATGGACAGTA+GGG 0.615566 2_2:+59299834 Msa0261130:CDS
AGATCAATGTTCAACTTGGG+AGG 0.619212 2_2:+59297502 Msa0261130:CDS
GTGGCAAAGAGGTCTTGAGA+AGG 0.619717 2_2:-59297540 None:intergenic
GCTCAAGTTATTTATCAACA+TGG 0.621098 2_2:+59301396 Msa0261130:CDS
GAGACCATCACCAATGAGGA+AGG 0.621909 2_2:-59300456 None:intergenic
CCTACTTCAGGACTAAGGTG+TGG 0.623254 2_2:-59299062 None:intergenic
CAAAGCACCTAAGAATGGCA+AGG 0.625330 2_2:+59299114 Msa0261130:CDS
TGGCAAAGAGGTCTTGAGAA+GGG 0.625651 2_2:-59297539 None:intergenic
GCCTGCAAATACAATGACAA+TGG 0.627016 2_2:+59295850 Msa0261130:exon
GGATGACTTAGGAACTTCAA+CGG 0.635407 2_2:+59300556 Msa0261130:CDS
TTGAGAGACCATCACCAATG+AGG 0.655213 2_2:-59300460 None:intergenic
AGATGATTGACTCAATCCAA+GGG 0.655644 2_2:+59297451 Msa0261130:CDS
ATTGAAGGGATGTGGCAAAG+AGG 0.660331 2_2:-59297551 None:intergenic
TCACTTACCAATGACATTTG+GGG 0.667503 2_2:+59298939 Msa0261130:CDS
ACCATCACCAATGAGGAAGG+TGG 0.672270 2_2:-59300453 None:intergenic
CAGGCTCTTCACTTGCTCCG+GGG 0.678340 2_2:+59300517 Msa0261130:CDS
AATTTGTGGAGAGAACTCAA+TGG 0.695889 2_2:+59301309 Msa0261130:CDS
GAAGCTTTGATAACAGACGA+AGG 0.721487 2_2:-59301354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTAATAAATAAA+GGG + chr2_2:59300599-59300618 Msa0261130:intron 0.0%
!! AAATTAGTTACTATAATTTA+GGG - chr2_2:59296893-59296912 None:intergenic 10.0%
!! AAAATTAGTTACTATAATTT+AGG - chr2_2:59296894-59296913 None:intergenic 10.0%
!!! AATCAGTTTTTAAAATAATA+TGG + chr2_2:59297227-59297246 Msa0261130:intron 10.0%
!! ACTCAATAAAAAAATAAATT+TGG - chr2_2:59297778-59297797 None:intergenic 10.0%
!! TTAAATATACATTATATAAG+AGG + chr2_2:59297941-59297960 Msa0261130:intron 10.0%
!!! TTTAATTAAATATTTTGTTG+TGG - chr2_2:59299293-59299312 None:intergenic 10.0%
!! ACAACAAAATATTTAATTAA+AGG + chr2_2:59299292-59299311 Msa0261130:intron 10.0%
!! AAAATTTCATCTTTAAATAT+CGG - chr2_2:59299573-59299592 None:intergenic 10.0%
!!! TTATTAATTAATTTTCAGTA+GGG - chr2_2:59300594-59300613 None:intergenic 10.0%
!!! TTTATTAATTAATTTTCAGT+AGG - chr2_2:59300595-59300614 None:intergenic 10.0%
!!! CAAATTTAAATTTTTATTAG+TGG + chr2_2:59300987-59301006 Msa0261130:intron 10.0%
!!! TTTTTCAAATAAAATACTAT+AGG - chr2_2:59301521-59301540 None:intergenic 10.0%
!!! TAGTATTTTATTTGAAAAAT+AGG + chr2_2:59301522-59301541 Msa0261130:three_prime_UTR 10.0%
!!! AGTATTTTATTTGAAAAATA+GGG + chr2_2:59301523-59301542 Msa0261130:three_prime_UTR 10.0%
!!! ATTGATGATTATATAAGATA+GGG - chr2_2:59296055-59296074 None:intergenic 15.0%
!!! TATTGATGATTATATAAGAT+AGG - chr2_2:59296056-59296075 None:intergenic 15.0%
!! TAAATTTAAATTCATTGTTG+TGG + chr2_2:59296290-59296309 Msa0261130:intron 15.0%
!! TTATTTGTAAAATTGTTTGA+AGG + chr2_2:59296599-59296618 Msa0261130:intron 15.0%
!!! AATTATAGTAACTAATTTTG+CGG + chr2_2:59296895-59296914 Msa0261130:intron 15.0%
!! AAATAAAAAGATGTGATTTA+CGG - chr2_2:59297185-59297204 None:intergenic 15.0%
!!! TCTTTTTATTTAGAAGTAAA+TGG + chr2_2:59297194-59297213 Msa0261130:intron 15.0%
!!! TCTAAGACAATATTTTATAA+GGG - chr2_2:59297275-59297294 None:intergenic 15.0%
!!! TTCTAAGACAATATTTTATA+AGG - chr2_2:59297276-59297295 None:intergenic 15.0%
!! AGAAATAAAAAGAATGAATT+TGG - chr2_2:59297888-59297907 None:intergenic 15.0%
!! TTAAGAAAATGTTTATTCTA+TGG - chr2_2:59298252-59298271 None:intergenic 15.0%
!!! CATTATATTTTTTTTTGACA+AGG + chr2_2:59298591-59298610 Msa0261130:intron 15.0%
!! ACAAAATATTTAATTAAAGG+TGG + chr2_2:59299295-59299314 Msa0261130:intron 15.0%
!!! ATTCAATATTGATTTAGATT+TGG - chr2_2:59299516-59299535 None:intergenic 15.0%
!! ATACATGAAAATATGTTATA+TGG + chr2_2:59300126-59300145 Msa0261130:CDS 15.0%
!!! CAAATTTAAATTTTTATCAG+TGG + chr2_2:59300747-59300766 Msa0261130:intron 15.0%
!!! AATATTTTTATTAGTGGTAA+AGG + chr2_2:59300814-59300833 Msa0261130:intron 15.0%
!!! TTTATTACAATTTGTTTCAT+AGG - chr2_2:59301129-59301148 None:intergenic 15.0%
!!! GATCATTTTTTTTTTTTTGA+AGG + chr2_2:59301622-59301641 Msa0261130:three_prime_UTR 15.0%
!!! TAATCATCAATATGAAGTTT+TGG + chr2_2:59296064-59296083 Msa0261130:intron 20.0%
!!! AATCATCAATATGAAGTTTT+GGG + chr2_2:59296065-59296084 Msa0261130:intron 20.0%
!!! ACTTGTCAAAATCAATTTTA+AGG - chr2_2:59296174-59296193 None:intergenic 20.0%
!!! ATATCAAACTGATTTATGTT+TGG - chr2_2:59296385-59296404 None:intergenic 20.0%
!! AAGTTGAATATAAAAGTTCT+AGG + chr2_2:59296436-59296455 Msa0261130:intron 20.0%
!! AGCATCTTCTTAAAAAATAA+AGG + chr2_2:59296498-59296517 Msa0261130:intron 20.0%
!! GCATCTTCTTAAAAAATAAA+GGG + chr2_2:59296499-59296518 Msa0261130:intron 20.0%
!! TAAATCTTATGTAGAAATGA+AGG + chr2_2:59296544-59296563 Msa0261130:intron 20.0%
!!! ACGATTTTTTATAGACAAAA+TGG - chr2_2:59296737-59296756 None:intergenic 20.0%
!!! TGTTTGTTTTAAGATGTTAT+TGG + chr2_2:59297097-59297116 Msa0261130:intron 20.0%
!!! GTTTGTTTTAAGATGTTATT+GGG + chr2_2:59297098-59297117 Msa0261130:intron 20.0%
!! TTATTGGGAATATTTGTATA+GGG - chr2_2:59298206-59298225 None:intergenic 20.0%
!! TTTATTGGGAATATTTGTAT+AGG - chr2_2:59298207-59298226 None:intergenic 20.0%
!!! CTGTATATGTTTGTTTTATT+GGG - chr2_2:59298221-59298240 None:intergenic 20.0%
!!! ACTGTATATGTTTGTTTTAT+TGG - chr2_2:59298222-59298241 None:intergenic 20.0%
!! TTTGTTCTCAAATAGTTTAA+TGG + chr2_2:59298293-59298312 Msa0261130:intron 20.0%
!! TGTAAGTATAATTCACTTAA+TGG - chr2_2:59298401-59298420 None:intergenic 20.0%
!!! TTTATAAGTATTGTATAAGC+AGG + chr2_2:59298778-59298797 Msa0261130:intron 20.0%
!! AGAAATATTAAAAAAAGCCA+TGG + chr2_2:59299007-59299026 Msa0261130:CDS 20.0%
!!! CTAGTTTATTTGATTTGATT+AGG + chr2_2:59299753-59299772 Msa0261130:intron 20.0%
!!! TAAACTTTATGTTTTACAGA+TGG + chr2_2:59300074-59300093 Msa0261130:intron 20.0%
!!! AAACTTTATGTTTTACAGAT+GGG + chr2_2:59300075-59300094 Msa0261130:intron 20.0%
!!! TAACATATTTTCATGTATTC+AGG - chr2_2:59300124-59300143 None:intergenic 20.0%
!! TCCAAATATGTCTTAAAATT+TGG - chr2_2:59300389-59300408 None:intergenic 20.0%
!!! TTTAAGACATATTTGGATAA+TGG + chr2_2:59300392-59300411 Msa0261130:CDS 20.0%
!! TATAAATTTGTATCTACTCA+AGG - chr2_2:59301039-59301058 None:intergenic 20.0%
!!! TCTTACTAATTTTTATGTTC+AGG + chr2_2:59301179-59301198 Msa0261130:intron 20.0%
!! AATATTTCAGATGAAAATGA+AGG + chr2_2:59301258-59301277 Msa0261130:CDS 20.0%
!!! TTTTCAACTCAATTTTTAGT+AGG - chr2_2:59301483-59301502 None:intergenic 20.0%
!!! TAAATGACATATTTTTGTCA+TGG + chr2_2:59301600-59301619 Msa0261130:three_prime_UTR 20.0%
!!! AGTGTGTTTGTTTTTACTAT+AGG + chr2_2:59296128-59296147 Msa0261130:intron 25.0%
!!! GTGTGTTTGTTTTTACTATA+GGG + chr2_2:59296129-59296148 Msa0261130:intron 25.0%
!!! GTCAAAATCAATTTTAAGGT+TGG - chr2_2:59296170-59296189 None:intergenic 25.0%
!!! AATTGATTTTGACAAGTTTG+TGG + chr2_2:59296177-59296196 Msa0261130:intron 25.0%
! AATTGCAAGTGATTTATGTT+TGG - chr2_2:59296332-59296351 None:intergenic 25.0%
! TTGCAATTCAATCAATTTCA+TGG + chr2_2:59296344-59296363 Msa0261130:intron 25.0%
! AATTCAATCAATTTCATGGT+TGG + chr2_2:59296348-59296367 Msa0261130:intron 25.0%
! CAAATAACACACTGTATAAT+TGG - chr2_2:59296586-59296605 None:intergenic 25.0%
! AATTTAGGGAAACTTGTATA+TGG - chr2_2:59296879-59296898 None:intergenic 25.0%
! ATCATCTTCAATGTTACAAT+AGG - chr2_2:59297438-59297457 None:intergenic 25.0%
! AAATAGTAATGCAATATACC+AGG - chr2_2:59297612-59297631 None:intergenic 25.0%
! TGGTATATTGCATTACTATT+TGG + chr2_2:59297611-59297630 Msa0261130:intron 25.0%
! CAATCAAAACATAATAGCAT+AGG + chr2_2:59297705-59297724 Msa0261130:intron 25.0%
!!! AAATCAGAGTTTTGATTTTG+TGG + chr2_2:59297966-59297985 Msa0261130:intron 25.0%
!!! AATCAGAGTTTTGATTTTGT+GGG + chr2_2:59297967-59297986 Msa0261130:intron 25.0%
! TATTGGGAATATTTGTATAG+GGG - chr2_2:59298205-59298224 None:intergenic 25.0%
!! TCTACACTTTTCTATATTCA+GGG - chr2_2:59298332-59298351 None:intergenic 25.0%
!! TTCTACACTTTTCTATATTC+AGG - chr2_2:59298333-59298352 None:intergenic 25.0%
! TCATGGATATTGAATATGTT+GGG - chr2_2:59298376-59298395 None:intergenic 25.0%
! TTCATGGATATTGAATATGT+TGG - chr2_2:59298377-59298396 None:intergenic 25.0%
!!! TTCTACTAAGTCTATTTTGA+TGG - chr2_2:59298493-59298512 None:intergenic 25.0%
! AAATAGACTTAGTAGAAATC+AGG + chr2_2:59298496-59298515 Msa0261130:intron 25.0%
!!! ACTAGTTTTTTACTGAAAGA+AGG - chr2_2:59298665-59298684 None:intergenic 25.0%
!!! AGATGTTTTCAGGATTTTTT+TGG + chr2_2:59298893-59298912 Msa0261130:intron 25.0%
!! TTATATGAGGCATCATATTT+AGG + chr2_2:59298972-59298991 Msa0261130:CDS 25.0%
!! TATATGAGGCATCATATTTA+GGG + chr2_2:59298973-59298992 Msa0261130:CDS 25.0%
!! TATGCAGGTATTTTATACTT+TGG + chr2_2:59299261-59299280 Msa0261130:intron 25.0%
! TCATAATTTCACACATTCAT+CGG + chr2_2:59299344-59299363 Msa0261130:intron 25.0%
!!! CGGTTGCTAATTTAATTTTT+TGG - chr2_2:59299553-59299572 None:intergenic 25.0%
!!! GTTTATTTGATTTGATTAGG+TGG + chr2_2:59299756-59299775 Msa0261130:intron 25.0%
! CATAACTAGCAAAATACATA+TGG - chr2_2:59299909-59299928 None:intergenic 25.0%
!! CATATGTATTTTGCTAGTTA+TGG + chr2_2:59299907-59299926 Msa0261130:CDS 25.0%
!! ACATAAAGTTTAGTTTAGCA+TGG - chr2_2:59300066-59300085 None:intergenic 25.0%
! TCTATATGCAATTTCATTTG+TGG - chr2_2:59300164-59300183 None:intergenic 25.0%
! ATTATTAACATGTCACTCTT+TGG - chr2_2:59300279-59300298 None:intergenic 25.0%
! CATGTTAATAATTGTAGACT+TGG + chr2_2:59300287-59300306 Msa0261130:intron 25.0%
!!! GCCAAATTTTAAGACATATT+TGG + chr2_2:59300385-59300404 Msa0261130:CDS 25.0%
!! AATTTGTTTCATAGGAATCA+TGG - chr2_2:59301121-59301140 None:intergenic 25.0%
!! TACTAATTTTTATGTTCAGG+AGG + chr2_2:59301182-59301201 Msa0261130:intron 25.0%
! ATGAAGGAAGAATACAAATA+AGG + chr2_2:59301274-59301293 Msa0261130:CDS 25.0%
! TATCAAAGCTTCTCTTAATA+TGG + chr2_2:59301365-59301384 Msa0261130:CDS 25.0%
! ACATTTGCTCGTTGTAATTT+TGG + chr2_2:59295908-59295927 Msa0261130:CDS 30.0%
! CATTTGCTCGTTGTAATTTT+GGG + chr2_2:59295909-59295928 Msa0261130:CDS 30.0%
CGCTACAAAAATCAAATTCA+AGG + chr2_2:59295960-59295979 Msa0261130:CDS 30.0%
TCAAATTCAAGGATTATGCA+TGG + chr2_2:59295971-59295990 Msa0261130:CDS 30.0%
AAAATAAAGGGTTATGCTAG+AGG + chr2_2:59296511-59296530 Msa0261130:intron 30.0%
! TTTTGAACTCAACTCTTACA+CGG - chr2_2:59296950-59296969 None:intergenic 30.0%
GTCTTATCTTCAGTTTGTAA+AGG - chr2_2:59297357-59297376 None:intergenic 30.0%
TGAAGATAAGACCAAAAGTT+TGG + chr2_2:59297365-59297384 Msa0261130:CDS 30.0%
GAAGATAAGACCAAAAGTTT+GGG + chr2_2:59297366-59297385 Msa0261130:CDS 30.0%
TTGAATTTAGCAATGCTTCT+CGG - chr2_2:59297410-59297429 None:intergenic 30.0%
ATGAGATCAATGTTCAACTT+GGG + chr2_2:59297499-59297518 Msa0261130:CDS 30.0%
TTCAAAGCATATACATGAAG+TGG - chr2_2:59297639-59297658 None:intergenic 30.0%
! CTTTGAATTTTCTCATGCTT+TGG + chr2_2:59297652-59297671 Msa0261130:intron 30.0%
AGAGGTCTAGGTTTATATAT+GGG - chr2_2:59297845-59297864 None:intergenic 30.0%
TAGAGGTCTAGGTTTATATA+TGG - chr2_2:59297846-59297865 None:intergenic 30.0%
!!! ATCAGAGTTTTGATTTTGTG+GGG + chr2_2:59297968-59297987 Msa0261130:intron 30.0%
! CACTTAAGTGATATACCTTT+TGG - chr2_2:59298001-59298020 None:intergenic 30.0%
ATATCACTTAAGTGAGTGTT+TGG + chr2_2:59298007-59298026 Msa0261130:intron 30.0%
!! TAATGACTTGTTTGGTTATG+CGG - chr2_2:59298152-59298171 None:intergenic 30.0%
!!! GTTTTGACTAATGACTTGTT+TGG - chr2_2:59298160-59298179 None:intergenic 30.0%
ATTGGGAATATTTGTATAGG+GGG - chr2_2:59298204-59298223 None:intergenic 30.0%
CATGGATATTGAATATGTTG+GGG - chr2_2:59298375-59298394 None:intergenic 30.0%
TAATTCACTTAATGGCTTCA+TGG - chr2_2:59298393-59298412 None:intergenic 30.0%
TACATGACATAATGACCTTA+TGG + chr2_2:59298417-59298436 Msa0261130:intron 30.0%
!! TTTTACTGAAAGAAGGTACA+AGG - chr2_2:59298658-59298677 None:intergenic 30.0%
CCAATAATACATGTCAGTAT+AGG - chr2_2:59298719-59298738 None:intergenic 30.0%
CCTATACTGACATGTATTAT+TGG + chr2_2:59298716-59298735 Msa0261130:intron 30.0%
! ACTTGATGATTTGATTAGTG+TGG + chr2_2:59298858-59298877 Msa0261130:intron 30.0%
ATTTGATTAGTGTGGTTGTT+TGG + chr2_2:59298866-59298885 Msa0261130:intron 30.0%
! GTTTGGTTATAGATGTTTTC+AGG + chr2_2:59298883-59298902 Msa0261130:intron 30.0%
AATCACTTACCAATGACATT+TGG + chr2_2:59298937-59298956 Msa0261130:CDS 30.0%
ATCACTTACCAATGACATTT+GGG + chr2_2:59298938-59298957 Msa0261130:CDS 30.0%
! AAAAAGCCATGGAATTTTCA+AGG + chr2_2:59299018-59299037 Msa0261130:CDS 30.0%
! AAAAGCCATGGAATTTTCAA+GGG + chr2_2:59299019-59299038 Msa0261130:CDS 30.0%
! TAACCAAATACCTACTCTTT+TGG + chr2_2:59299178-59299197 Msa0261130:CDS 30.0%
ATTCCAAAAGAGTAGGTATT+TGG - chr2_2:59299184-59299203 None:intergenic 30.0%
!!! AATACCTACTCTTTTGGAAT+TGG + chr2_2:59299184-59299203 Msa0261130:CDS 30.0%
! ATATTTCTGCCAATTCTCTT+TGG - chr2_2:59299244-59299263 None:intergenic 30.0%
AAAGGTGGAAGAAATTGATT+TGG + chr2_2:59299310-59299329 Msa0261130:intron 30.0%
TTCATGGCAGAAATTAATGT+TGG - chr2_2:59299670-59299689 None:intergenic 30.0%
! AGTAGACAAAAAGAAGTATG+AGG - chr2_2:59299702-59299721 None:intergenic 30.0%
!! TATTTGATTTGATTAGGTGG+TGG + chr2_2:59299759-59299778 Msa0261130:intron 30.0%
! TGGTTTTTGTAAGTTCAATG+CGG - chr2_2:59299889-59299908 None:intergenic 30.0%
GATGATATCTTTGACACTTA+TGG + chr2_2:59299929-59299948 Msa0261130:CDS 30.0%
!! TGAACTTGTTCTTTTCACAA+AGG + chr2_2:59299961-59299980 Msa0261130:CDS 30.0%
! TTTTCACAAAGGCAATCAAA+AGG + chr2_2:59299972-59299991 Msa0261130:CDS 30.0%
TATAGAATCCAGAAAGAACA+TGG + chr2_2:59300178-59300197 Msa0261130:CDS 30.0%
!!! TCTTTTCAGGTAAGAAACAA+CGG - chr2_2:59300209-59300228 None:intergenic 30.0%
! AGATTCTTACTGTTCTTTTC+AGG - chr2_2:59300222-59300241 None:intergenic 30.0%
CATTTCTTGAAGAAGCTAAA+TGG + chr2_2:59300345-59300364 Msa0261130:CDS 30.0%
GAAGCTAAATGGTTCAATAA+TGG + chr2_2:59300356-59300375 Msa0261130:CDS 30.0%
ATACAAATAAGGATGCTACT+AGG + chr2_2:59301285-59301304 Msa0261130:CDS 30.0%
TACAAATAAGGATGCTACTA+GGG + chr2_2:59301286-59301305 Msa0261130:CDS 30.0%
GCTCAAGTTATTTATCAACA+TGG + chr2_2:59301396-59301415 Msa0261130:CDS 30.0%
AGTTTGTGGAATGTTGTAGT+TGG + chr2_2:59296191-59296210 Msa0261130:intron 35.0%
AGAAGATGCTAAAAGACACT+TGG - chr2_2:59296488-59296507 None:intergenic 35.0%
TGAGACAAAAAGATGTGTTG+TGG - chr2_2:59296684-59296703 None:intergenic 35.0%
GTGTGAGTGCTACATTTAAT+TGG + chr2_2:59296923-59296942 Msa0261130:intron 35.0%
TAGTAAAACAAAACCAAGCC+AGG - chr2_2:59297004-59297023 None:intergenic 35.0%
! ATTTTCTCCACATGTGTTGT+AGG - chr2_2:59297039-59297058 None:intergenic 35.0%
!!! TTTGACTTGACCCAAACTTT+TGG - chr2_2:59297379-59297398 None:intergenic 35.0%
! AAGATGATTGACTCAATCCA+AGG + chr2_2:59297450-59297469 Msa0261130:CDS 35.0%
! AGATGATTGACTCAATCCAA+GGG + chr2_2:59297451-59297470 Msa0261130:CDS 35.0%
CATTGATCTCATCTTCCAAA+TGG - chr2_2:59297491-59297510 None:intergenic 35.0%
GATGAGATCAATGTTCAACT+TGG + chr2_2:59297498-59297517 Msa0261130:CDS 35.0%
ACTCTCTTGACATGACAAAA+TGG - chr2_2:59297679-59297698 None:intergenic 35.0%
GTTCTAATATGTAGAGGTCT+AGG - chr2_2:59297857-59297876 None:intergenic 35.0%
!!! TCAGAGTTTTGATTTTGTGG+GGG + chr2_2:59297969-59297988 Msa0261130:intron 35.0%
! TGAGTGTTTGGATCAACTTT+TGG + chr2_2:59298019-59298038 Msa0261130:intron 35.0%
TTCCACGTTGAAATTGCATT+GGG - chr2_2:59298061-59298080 None:intergenic 35.0%
AGCTCATATTTGTAGCTTCA+TGG - chr2_2:59298084-59298103 None:intergenic 35.0%
AGCTTCATCTTGTATCCATA+AGG - chr2_2:59298435-59298454 None:intergenic 35.0%
!! ATTTTGATGGCTAAGTGCAA+GGG - chr2_2:59298480-59298499 None:intergenic 35.0%
!!! TATTTTGATGGCTAAGTGCA+AGG - chr2_2:59298481-59298500 None:intergenic 35.0%
AATACATGTCAGTATAGGTG+TGG - chr2_2:59298714-59298733 None:intergenic 35.0%
!!! AGGATTTTTTTGGACAAGAG+TGG + chr2_2:59298903-59298922 Msa0261130:CDS 35.0%
TCACTTACCAATGACATTTG+GGG + chr2_2:59298939-59298958 Msa0261130:CDS 35.0%
GGGTATGTTGAGTTTATATG+AGG + chr2_2:59298959-59298978 Msa0261130:CDS 35.0%
TTTGCCAATTCCAAAAGAGT+AGG - chr2_2:59299191-59299210 None:intergenic 35.0%
! ATTCTCTTTGGTGTAATGAC+TGG - chr2_2:59299232-59299251 None:intergenic 35.0%
GTCATTACACCAAAGAGAAT+TGG + chr2_2:59299232-59299251 Msa0261130:CDS 35.0%
ACACATTCATCGGTAGTAAT+AGG + chr2_2:59299354-59299373 Msa0261130:intron 35.0%
AGGATGATGATTGTAGAGTT+CGG + chr2_2:59299374-59299393 Msa0261130:intron 35.0%
TTTGTCTATTCTCGATCCAA+TGG + chr2_2:59299452-59299471 Msa0261130:intron 35.0%
ATAAATGTCACGCATTCACA+TGG - chr2_2:59299486-59299505 None:intergenic 35.0%
TTCCTTGTCGCTAAAATTAG+TGG - chr2_2:59299617-59299636 None:intergenic 35.0%
TATGAGGTTTGTGATGTTCA+TGG - chr2_2:59299686-59299705 None:intergenic 35.0%
! TTTTGCAAGAGATAGACCAA+CGG + chr2_2:59299811-59299830 Msa0261130:CDS 35.0%
TGTCACTCTTTGGTATACTT+TGG - chr2_2:59300269-59300288 None:intergenic 35.0%
!! TTGGTGATTAATTTGTGCAG+TGG + chr2_2:59300306-59300325 Msa0261130:intron 35.0%
AATTTGTGGAGAGAACTCAA+TGG + chr2_2:59301309-59301328 Msa0261130:CDS 35.0%
!! AACAGACGAAGGTATGTTTT+TGG - chr2_2:59301346-59301365 None:intergenic 35.0%
AGGTAATGATGTGAAGATCA+AGG - chr2_2:59301463-59301482 None:intergenic 35.0%
GCCTGCAAATACAATGACAA+TGG + chr2_2:59295850-59295869 Msa0261130:exon 40.0%
GCCATTGTCATTGTATTTGC+AGG - chr2_2:59295854-59295873 None:intergenic 40.0%
TACCTTCTCATTGAGAGCAA+TGG - chr2_2:59296014-59296033 None:intergenic 40.0%
! AGTAACTAATTTTGCGGCCT+AGG + chr2_2:59296901-59296920 Msa0261130:intron 40.0%
TAAATGTAGCACTCACACCT+AGG - chr2_2:59296921-59296940 None:intergenic 40.0%
TAAAACAAAACCAAGCCAGG+TGG - chr2_2:59297001-59297020 None:intergenic 40.0%
GAGAATTCCTACAACACATG+TGG + chr2_2:59297029-59297048 Msa0261130:intron 40.0%
AGATCAATGTTCAACTTGGG+AGG + chr2_2:59297502-59297521 Msa0261130:CDS 40.0%
AACTTGGGAGGATATGTGAT+TGG + chr2_2:59297514-59297533 Msa0261130:CDS 40.0%
ACTTGGGAGGATATGTGATT+GGG + chr2_2:59297515-59297534 Msa0261130:CDS 40.0%
TTGCCACATCCCTTCAATTT+CGG + chr2_2:59297556-59297575 Msa0261130:CDS 40.0%
TAACCGAAATTGAAGGGATG+TGG - chr2_2:59297562-59297581 None:intergenic 40.0%
CCTCAGTAACCGAAATTGAA+GGG - chr2_2:59297568-59297587 None:intergenic 40.0%
TTTCGGTTACTGAGGCATAA+TGG + chr2_2:59297573-59297592 Msa0261130:CDS 40.0%
TGCAATATACCAGGACATGT+GGG - chr2_2:59297603-59297622 None:intergenic 40.0%
ATGCAATATACCAGGACATG+TGG - chr2_2:59297604-59297623 None:intergenic 40.0%
GACCTCTACATATTAGAACG+TGG + chr2_2:59297858-59297877 Msa0261130:intron 40.0%
CTCCACGTTCTAATATGTAG+AGG - chr2_2:59297863-59297882 None:intergenic 40.0%
GTTCCACGTTGAAATTGCAT+TGG - chr2_2:59298062-59298081 None:intergenic 40.0%
TGGATACAAGATGAAGCTCT+AGG + chr2_2:59298437-59298456 Msa0261130:intron 40.0%
! TGGACAAGAGTGGCAATTTT+AGG + chr2_2:59298913-59298932 Msa0261130:CDS 40.0%
! GGACAAGAGTGGCAATTTTA+GGG + chr2_2:59298914-59298933 Msa0261130:CDS 40.0%
CAACATACCCCAAATGTCAT+TGG - chr2_2:59298949-59298968 None:intergenic 40.0%
!! CATTCTTAGGTGCTTTGGAA+GGG - chr2_2:59299111-59299130 None:intergenic 40.0%
CTTCTAACCTTGCCATTCTT+AGG - chr2_2:59299124-59299143 None:intergenic 40.0%
GAGAATTGGCAGAAATATGC+AGG + chr2_2:59299246-59299265 Msa0261130:CDS 40.0%
!! TGCCACTAATTTTAGCGACA+AGG + chr2_2:59299612-59299631 Msa0261130:intron 40.0%
!! ATTAGGTGGTGGAAAGAGTT+GGG + chr2_2:59299770-59299789 Msa0261130:intron 40.0%
!! TTGGGACTTGTTGACAAACT+TGG + chr2_2:59299788-59299807 Msa0261130:CDS 40.0%
AGTGCTTCTTATGGACAGTA+GGG + chr2_2:59299834-59299853 Msa0261130:CDS 40.0%
CAATTTGAATGACGTGGTTC+TGG - chr2_2:59299866-59299885 None:intergenic 40.0%
CACAAAGGCAATCAAAAGGT+TGG + chr2_2:59299976-59299995 Msa0261130:intron 40.0%
GAAGTTGTTCCATTGCATCA+AGG - chr2_2:59300102-59300121 None:intergenic 40.0%
!! GATAATGGAGTGATTTCTGC+AGG + chr2_2:59300407-59300426 Msa0261130:CDS 40.0%
GATTTCTGCAGGATCATGTA+TGG + chr2_2:59300418-59300437 Msa0261130:CDS 40.0%
GGGATATGACTTCATCATCA+TGG - chr2_2:59300498-59300517 None:intergenic 40.0%
! TGATGATGAAGTCATATCCC+AGG + chr2_2:59300498-59300517 Msa0261130:CDS 40.0%
GGATGACTTAGGAACTTCAA+CGG + chr2_2:59300556-59300575 Msa0261130:CDS 40.0%
!!! CGAAGGTATGTTTTTGGTCA+TGG - chr2_2:59301340-59301359 None:intergenic 40.0%
! GAAGCTTTGATAACAGACGA+AGG - chr2_2:59301357-59301376 None:intergenic 40.0%
GTCCATTGCTCTCAATGAGA+AGG + chr2_2:59296009-59296028 Msa0261130:CDS 45.0%
TAGAGTGAATTGCAACCACC+TGG + chr2_2:59296983-59297002 Msa0261130:intron 45.0%
! TGGGCAGCAGCAACATTTTA+CGG + chr2_2:59297117-59297136 Msa0261130:intron 45.0%
! ATTGACTCAATCCAAGGGCT+GGG + chr2_2:59297456-59297475 Msa0261130:CDS 45.0%
TGGCAAAGAGGTCTTGAGAA+GGG - chr2_2:59297542-59297561 None:intergenic 45.0%
ATTGAAGGGATGTGGCAAAG+AGG - chr2_2:59297554-59297573 None:intergenic 45.0%
CCCTTCAATTTCGGTTACTG+AGG + chr2_2:59297565-59297584 Msa0261130:CDS 45.0%
GCCTCAGTAACCGAAATTGA+AGG - chr2_2:59297569-59297588 None:intergenic 45.0%
TTGTGGGGGACAAAACCAAA+AGG + chr2_2:59297983-59298002 Msa0261130:intron 45.0%
GTCCCAATGCAATTTCAACG+TGG + chr2_2:59298056-59298075 Msa0261130:intron 45.0%
AGTGCAAGGGTGGAAACATT+GGG - chr2_2:59298467-59298486 None:intergenic 45.0%
AAGTGCAAGGGTGGAAACAT+TGG - chr2_2:59298468-59298487 None:intergenic 45.0%
ACGAGCCCTTGAAAATTCCA+TGG - chr2_2:59299027-59299046 None:intergenic 45.0%
CTACTTCAGGACTAAGGTGT+GGG - chr2_2:59299064-59299083 None:intergenic 45.0%
!! TAGGTGCTTTGGAAGGGTTA+AGG - chr2_2:59299105-59299124 None:intergenic 45.0%
! CCATTCTTAGGTGCTTTGGA+AGG - chr2_2:59299112-59299131 None:intergenic 45.0%
! CCTTCCAAAGCACCTAAGAA+TGG + chr2_2:59299109-59299128 Msa0261130:CDS 45.0%
CTTGCCATTCTTAGGTGCTT+TGG - chr2_2:59299116-59299135 None:intergenic 45.0%
! CAAAGCACCTAAGAATGGCA+AGG + chr2_2:59299114-59299133 Msa0261130:CDS 45.0%
TCACATGGTCAAGAGACCAT+TGG - chr2_2:59299471-59299490 None:intergenic 45.0%
!! GATTAGGTGGTGGAAAGAGT+TGG + chr2_2:59299769-59299788 Msa0261130:intron 45.0%
GAGTGCTTCTTATGGACAGT+AGG + chr2_2:59299833-59299852 Msa0261130:CDS 45.0%
ATGCGGCAATTTGAATGACG+TGG - chr2_2:59299872-59299891 None:intergenic 45.0%
! CGGTTAGTCCATGTTCTTTC+TGG - chr2_2:59300189-59300208 None:intergenic 45.0%
TTGAGAGACCATCACCAATG+AGG - chr2_2:59300463-59300482 None:intergenic 45.0%
!! CATTGGTGATGGTCTCTCAA+AGG + chr2_2:59300463-59300482 Msa0261130:CDS 45.0%
CCATAGTCGAAGAATTTCCC+CGG - chr2_2:59300537-59300556 None:intergenic 45.0%
CGGGGAAATTCTTCGACTAT+GGG + chr2_2:59300535-59300554 Msa0261130:CDS 45.0%
CTTCGACTATGGGATGACTT+AGG + chr2_2:59300545-59300564 Msa0261130:CDS 45.0%
! GTCATGCTAACTTGTGCTCT+GGG + chr2_2:59300621-59300640 Msa0261130:intron 45.0%
! TCATGCTAACTTGTGCTCTG+GGG + chr2_2:59300622-59300641 Msa0261130:intron 45.0%
GTTCAGGAGGAACAAGAAAG+AGG + chr2_2:59301195-59301214 Msa0261130:intron 45.0%
GGATGCTACTAGGGAATTTG+TGG + chr2_2:59301295-59301314 Msa0261130:CDS 45.0%
!!! ATTTCATATATTTAATTTAA+CGG - chr2_2:59297822-59297841 None:intergenic 5.0%
!! GAAAATTAATTAATAAATAA+AGG + chr2_2:59300598-59300617 Msa0261130:intron 5.0%
!!! AATTTAAATATTTTTATTAG+TGG + chr2_2:59300808-59300827 Msa0261130:intron 5.0%
TGAATTGCAACCACCTGGCT+TGG + chr2_2:59296988-59297007 Msa0261130:intron 50.0%
! GATTGACTCAATCCAAGGGC+TGG + chr2_2:59297455-59297474 Msa0261130:CDS 50.0%
TTGACTCAATCCAAGGGCTG+GGG + chr2_2:59297457-59297476 Msa0261130:CDS 50.0%
GTGGCAAAGAGGTCTTGAGA+AGG - chr2_2:59297543-59297562 None:intergenic 50.0%
GGTTACTGAGGCATAATGGC+TGG + chr2_2:59297577-59297596 Msa0261130:CDS 50.0%
GTGCAAGGGTGGAAACATTG+GGG - chr2_2:59298466-59298485 None:intergenic 50.0%
! TTGATGGCTAAGTGCAAGGG+TGG - chr2_2:59298477-59298496 None:intergenic 50.0%
CCTACTTCAGGACTAAGGTG+TGG - chr2_2:59299065-59299084 None:intergenic 50.0%
CCACACCTTAGTCCTGAAGT+AGG + chr2_2:59299062-59299081 Msa0261130:CDS 50.0%
ACCTTAGTCCTGAAGTAGGC+AGG + chr2_2:59299066-59299085 Msa0261130:CDS 50.0%
CCAATTTGCCTGCCTACTTC+AGG - chr2_2:59299077-59299096 None:intergenic 50.0%
CCTGAAGTAGGCAGGCAAAT+TGG + chr2_2:59299074-59299093 Msa0261130:CDS 50.0%
! GACCAACGGAGTGCTTCTTA+TGG + chr2_2:59299825-59299844 Msa0261130:CDS 50.0%
!! GTCCATAAGAAGCACTCCGT+TGG - chr2_2:59299830-59299849 None:intergenic 50.0%
CAGATGGGACCTTGATGCAA+TGG + chr2_2:59300090-59300109 Msa0261130:intron 50.0%
ATGCATGCCACCTTCCTCAT+TGG + chr2_2:59300446-59300465 Msa0261130:CDS 50.0%
ACCATCACCAATGAGGAAGG+TGG - chr2_2:59300456-59300475 None:intergenic 50.0%
GAGACCATCACCAATGAGGA+AGG - chr2_2:59300459-59300478 None:intergenic 50.0%
CCGGGGAAATTCTTCGACTA+TGG + chr2_2:59300534-59300553 Msa0261130:CDS 50.0%
! GGTCATGCTAACTTGTGCTC+TGG + chr2_2:59300620-59300639 Msa0261130:intron 50.0%
TCAATCCAAGGGCTGGGGAT+TGG + chr2_2:59297462-59297481 Msa0261130:CDS 55.0%
!! GCTGGGGATTGGTCACCATT+TGG + chr2_2:59297473-59297492 Msa0261130:CDS 55.0%
GCCTGCCTACTTCAGGACTA+AGG - chr2_2:59299070-59299089 None:intergenic 55.0%
GCCACCTTCCTCATTGGTGA+TGG + chr2_2:59300452-59300471 Msa0261130:CDS 55.0%
! AATGGCTGGCCCACATGTCC+TGG + chr2_2:59297591-59297610 Msa0261130:CDS 60.0%
CCGGAGCAAGTGAAGAGCCT+GGG - chr2_2:59300518-59300537 None:intergenic 60.0%
CCCAGGCTCTTCACTTGCTC+CGG + chr2_2:59300515-59300534 Msa0261130:CDS 60.0%
CCAGGCTCTTCACTTGCTCC+GGG + chr2_2:59300516-59300535 Msa0261130:CDS 60.0%
CAGGCTCTTCACTTGCTCCG+GGG + chr2_2:59300517-59300536 Msa0261130:CDS 60.0%
GGTGACCAATCCCCAGCCCT+TGG - chr2_2:59297470-59297489 None:intergenic 65.0%
CCCGGAGCAAGTGAAGAGCC+TGG - chr2_2:59300519-59300538 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 59295840 59301654 59295840 ID=Msa0261130;Name=Msa0261130
chr2_2 mRNA 59295840 59301654 59295840 ID=Msa0261130-mRNA-1;Parent=Msa0261130;Name=Msa0261130-mRNA-1;_AED=0.29;_eAED=0.29;_QI=23|1|1|1|1|1|7|160|569
chr2_2 exon 59295840 59296030 59295840 ID=Msa0261130-mRNA-1:exon:18294;Parent=Msa0261130-mRNA-1
chr2_2 exon 59297348 59297612 59297348 ID=Msa0261130-mRNA-1:exon:18295;Parent=Msa0261130-mRNA-1
chr2_2 exon 59298895 59299267 59298895 ID=Msa0261130-mRNA-1:exon:18296;Parent=Msa0261130-mRNA-1
chr2_2 exon 59299775 59299993 59299775 ID=Msa0261130-mRNA-1:exon:18297;Parent=Msa0261130-mRNA-1
chr2_2 exon 59300093 59300231 59300093 ID=Msa0261130-mRNA-1:exon:18298;Parent=Msa0261130-mRNA-1
chr2_2 exon 59300326 59300577 59300326 ID=Msa0261130-mRNA-1:exon:18299;Parent=Msa0261130-mRNA-1
chr2_2 exon 59301201 59301654 59301201 ID=Msa0261130-mRNA-1:exon:18300;Parent=Msa0261130-mRNA-1
chr2_2 five_prime_UTR 59295840 59295862 59295840 ID=Msa0261130-mRNA-1:five_prime_utr;Parent=Msa0261130-mRNA-1
chr2_2 CDS 59295863 59296030 59295863 ID=Msa0261130-mRNA-1:cds;Parent=Msa0261130-mRNA-1
chr2_2 CDS 59297348 59297612 59297348 ID=Msa0261130-mRNA-1:cds;Parent=Msa0261130-mRNA-1
chr2_2 CDS 59298895 59299267 59298895 ID=Msa0261130-mRNA-1:cds;Parent=Msa0261130-mRNA-1
chr2_2 CDS 59299775 59299993 59299775 ID=Msa0261130-mRNA-1:cds;Parent=Msa0261130-mRNA-1
chr2_2 CDS 59300093 59300231 59300093 ID=Msa0261130-mRNA-1:cds;Parent=Msa0261130-mRNA-1
chr2_2 CDS 59300326 59300577 59300326 ID=Msa0261130-mRNA-1:cds;Parent=Msa0261130-mRNA-1
chr2_2 CDS 59301201 59301494 59301201 ID=Msa0261130-mRNA-1:cds;Parent=Msa0261130-mRNA-1
chr2_2 three_prime_UTR 59301495 59301654 59301495 ID=Msa0261130-mRNA-1:three_prime_utr;Parent=Msa0261130-mRNA-1
Gene Sequence

>Msa0261130

ATGACAATGGCTGTTTCTGAACTTAGAGTTTCATATCTAACAAGCACATTTGCTCGTTGTAATTTTGGGACTTCTACATATCAATCACGCATCTTATCGCTACAAAAATCAAATTCAAGGATTATGCATGGTAAATTATCGATCAAGTCCATTGCTCTCAATGAGAAGTTGTCACCTTTACAAACTGAAGATAAGACCAAAAGTTTGGGTCAAGTCAAAAGAAGAAGCCGAGAAGCATTGCTAAATTCAAATGATCCTATTGTAACATTGAAGATGATTGACTCAATCCAAGGGCTGGGGATTGGTCACCATTTGGAAGATGAGATCAATGTTCAACTTGGGAGGATATGTGATTGGGACCCTTCTCAAGACCTCTTTGCCACATCCCTTCAATTTCGGTTACTGAGGCATAATGGCTGGCCCACATGTCCTGATGTTTTCAGGATTTTTTTGGACAAGAGTGGCAATTTTAGGGAATCACTTACCAATGACATTTGGGGTATGTTGAGTTTATATGAGGCATCATATTTAGGGACAGAAGATGAAGAAATATTAAAAAAAGCCATGGAATTTTCAAGGGCTCGTCTAAGTGAATTAATCCCACACCTTAGTCCTGAAGTAGGCAGGCAAATTGGAAGAGCCTTAACCCTTCCAAAGCACCTAAGAATGGCAAGGTTAGAAGCTAGAAACTACATTGAAGAATATAGTCAAGAAAGTAACCAAATACCTACTCTTTTGGAATTGGCAAAGTTAGACTCTGAGATGATCCAGTCATTACACCAAAGAGAATTGGCAGAAATATGCAGGTGGTGGAAAGAGTTGGGACTTGTTGACAAACTTGGTTTTGCAAGAGATAGACCAACGGAGTGCTTCTTATGGACAGTAGGGATATTTCCAGAACCACGTCATTCAAATTGCCGCATTGAACTTACAAAAACCATATGTATTTTGCTAGTTATGGATGATATCTTTGACACTTATGGCACATTAGATGAACTTGTTCTTTTCACAAAGGCAATCAAAAGATGGGACCTTGATGCAATGGAACAACTTCCTGAATACATGAAAATATGTTATATGGCATTATATAACACCACAAATGAAATTGCATATAGAATCCAGAAAGAACATGGACTAACCGTTGTTTCTTACCTGAAAAGAACATGGATTGACATATTTGAAGCATTTCTTGAAGAAGCTAAATGGTTCAATAATGGAAGTGTGCCAAATTTTAAGACATATTTGGATAATGGAGTGATTTCTGCAGGATCATGTATGGCATTGATGCATGCCACCTTCCTCATTGGTGATGGTCTCTCAAAGGAAACTATTTCCATGATGATGAAGTCATATCCCAGGCTCTTCACTTGCTCCGGGGAAATTCTTCGACTATGGGATGACTTAGGAACTTCAACGGAGGAACAAGAAAGAGGAGATAATGCTAGCAGTATACAATGCATGATGAGAGAGAATAATATTTCAGATGAAAATGAAGGAAGAATACAAATAAGGATGCTACTAGGGAATTTGTGGAGAGAACTCAATGGTCTTGCCATGACCAAAAACATACCTTCGTCTGTTATCAAAGCTTCTCTTAATATGGCTAGAACTGCTCAAGTTATTTATCAACATGGAGATGATCAAAGCACATTTACTGTTGATGACTATGTGCAAACCTTGATCTTCACATCATTACCTACTAAAAATTGA
Protein sequence

>Msa0261130

MTMAVSELRVSYLTSTFARCNFGTSTYQSRILSLQKSNSRIMHGKLSIKSIALNEKLSPLQTEDKTKSLGQVKRRSREALLNSNDPIVTLKMIDSIQGLGIGHHLEDEINVQLGRICDWDPSQDLFATSLQFRLLRHNGWPTCPDVFRIFLDKSGNFRESLTNDIWGMLSLYEASYLGTEDEEILKKAMEFSRARLSELIPHLSPEVGRQIGRALTLPKHLRMARLEARNYIEEYSQESNQIPTLLELAKLDSEMIQSLHQRELAEICRWWKELGLVDKLGFARDRPTECFLWTVGIFPEPRHSNCRIELTKTICILLVMDDIFDTYGTLDELVLFTKAIKRWDLDAMEQLPEYMKICYMALYNTTNEIAYRIQKEHGLTVVSYLKRTWIDIFEAFLEEAKWFNNGSVPNFKTYLDNGVISAGSCMALMHATFLIGDGLSKETISMMMKSYPRLFTCSGEILRLWDDLGTSTEEQERGDNASSIQCMMRENNISDENEGRIQIRMLLGNLWRELNGLAMTKNIPSSVIKASLNMARTAQVIYQHGDDQSTFTVDDYVQTLIFTSLPTKN*