AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0336680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336680 MtrunA17_Chr2g0323131 92.945 567 40 0 3 569 1 567 0.0 1103
Msa0336680 MtrunA17_Chr2g0323151 85.000 480 71 1 87 566 1 479 0.0 872
Msa0336680 MtrunA17_Chr4g0023911 39.879 494 277 8 64 546 74 558 3.13e-116 357
Msa0336680 MtrunA17_Chr4g0042111 36.634 505 299 7 57 546 41 539 5.58e-109 337
Msa0336680 MtrunA17_Chr2g0310331 35.943 562 348 6 15 566 26 585 3.07e-108 336
Msa0336680 MtrunA17_Chr4g0048911 33.453 559 344 11 21 561 6 554 2.75e-104 325
Msa0336680 MtrunA17_Chr4g0048921 35.686 510 314 7 62 562 62 566 5.03e-101 317
Msa0336680 MtrunA17_Chr6g0451711 34.870 499 303 8 83 566 65 556 6.24e-98 308
Msa0336680 MtrunA17_Chr2g0310401 39.675 431 250 5 142 566 5 431 1.67e-97 303
Msa0336680 MtrunA17_Chr4g0024691 34.398 532 322 11 49 566 42 560 2.27e-96 304
Msa0336680 MtrunA17_Chr6g0465441 32.549 510 329 8 69 566 46 552 4.75e-95 301
Msa0336680 MtrunA17_Chr8g0357411 34.470 528 333 7 50 566 63 588 7.25e-95 301
Msa0336680 MtrunA17_Chr7g0236961 34.454 476 294 6 86 549 9 478 1.75e-94 298
Msa0336680 MtrunA17_Chr3g0099031 33.333 501 320 6 74 561 13 512 1.81e-94 298
Msa0336680 MtrunA17_Chr7g0237011 34.454 476 294 6 86 549 9 478 2.84e-94 297
Msa0336680 MtrunA17_Chr2g0280581 33.004 506 326 4 69 561 12 517 3.61e-94 297
Msa0336680 MtrunA17_Chr7g0236991 33.943 492 306 7 69 547 46 531 5.27e-94 298
Msa0336680 MtrunA17_Chr7g0237001 33.943 492 306 7 69 547 46 531 8.33e-94 297
Msa0336680 MtrunA17_Chr7g0237041 33.943 492 306 7 69 547 46 531 8.33e-94 297
Msa0336680 MtrunA17_Chr7g0237081 33.943 492 306 7 69 547 46 531 8.33e-94 297
Msa0336680 MtrunA17_Chr7g0237051 33.943 492 306 7 69 547 46 531 8.33e-94 297
Msa0336680 MtrunA17_Chr3g0109001 33.399 506 318 5 72 561 11 513 6.77e-93 294
Msa0336680 MtrunA17_Chr3g0108941 33.855 511 317 7 72 565 11 517 2.33e-92 293
Msa0336680 MtrunA17_Chr5g0425371 34.247 511 313 9 72 566 49 552 4.44e-92 293
Msa0336680 MtrunA17_Chr3g0108981 33.538 489 306 5 89 561 3 488 2.00e-91 290
Msa0336680 MtrunA17_Chr3g0108961 33.538 489 306 5 89 561 3 488 2.00e-91 290
Msa0336680 MtrunA17_Chr3g0108991 33.792 509 319 9 72 565 11 516 7.85e-91 289
Msa0336680 MtrunA17_Chr3g0108951 33.792 509 319 9 72 565 11 516 7.85e-91 289
Msa0336680 MtrunA17_Chr3g0109021 33.400 503 317 9 74 561 13 512 1.18e-90 288
Msa0336680 MtrunA17_Chr6g0477111 34.568 486 298 7 92 561 6 487 1.66e-90 287
Msa0336680 MtrunA17_Chr3g0105071 34.583 480 291 7 83 547 64 535 1.70e-90 289
Msa0336680 MtrunA17_Chr3g0108971 34.553 492 304 9 89 565 3 491 6.62e-90 285
Msa0336680 MtrunA17_Chr3g0082691 33.068 502 311 8 82 566 59 552 1.35e-89 286
Msa0336680 MtrunA17_Chr3g0109031 31.683 505 331 5 74 565 13 516 3.65e-89 284
Msa0336680 MtrunA17_Chr2g0319051 34.127 504 313 9 73 561 9 508 5.85e-89 284
Msa0336680 MtrunA17_Chr3g0105691 33.943 492 311 7 86 564 58 548 8.76e-89 284
Msa0336680 MtrunA17_Chr6g0465851 32.227 512 328 7 69 566 11 517 4.55e-88 281
Msa0336680 MtrunA17_Chr6g0465911 32.227 512 328 7 69 566 46 552 8.70e-88 282
Msa0336680 MtrunA17_Chr6g0466051 32.227 512 328 7 69 566 46 552 9.47e-88 281
Msa0336680 MtrunA17_Chr5g0431651 32.738 504 310 8 82 566 58 551 4.07e-87 280
Msa0336680 MtrunA17_Chr3g0109011 32.924 489 308 7 89 561 3 487 4.65e-87 278
Msa0336680 MtrunA17_Chr2g0319041 32.184 522 330 11 59 561 35 551 2.59e-86 278
Msa0336680 MtrunA17_Chr5g0431571 34.343 495 299 9 72 549 44 529 2.45e-84 273
Msa0336680 MtrunA17_Chr8g0336181 33.333 510 308 11 62 550 39 537 1.83e-83 271
Msa0336680 MtrunA17_Chr6g0477121 35.422 415 256 6 143 549 8 418 3.84e-81 260
Msa0336680 MtrunA17_Chr7g0236951 39.871 311 174 2 86 386 9 316 5.26e-75 241
Msa0336680 MtrunA17_Chr7g0237031 39.871 311 174 2 86 386 9 316 1.04e-74 240
Msa0336680 MtrunA17_Chr7g0236971 34.115 384 248 4 168 549 1 381 1.82e-74 242
Msa0336680 MtrunA17_Chr6g0466111 38.788 330 188 3 69 387 11 337 1.82e-71 232
Msa0336680 MtrunA17_Chr6g0465981 39.683 315 177 2 83 387 61 372 4.52e-71 233
Msa0336680 MtrunA17_Chr6g0465871 39.683 315 177 2 83 387 61 372 4.52e-71 233
Msa0336680 MtrunA17_Chr6g0466081 39.683 315 177 2 83 387 61 372 4.52e-71 233
Msa0336680 MtrunA17_Chr6g0465931 39.935 308 172 2 90 387 33 337 2.98e-70 229
Msa0336680 MtrunA17_Chr4g0008621 34.831 356 220 4 84 428 70 424 2.48e-63 214
Msa0336680 MtrunA17_Chr7g0217361 39.007 282 168 4 267 546 8 287 1.16e-60 203
Msa0336680 MtrunA17_Chr5g0445511 40.336 238 142 0 164 401 2 239 8.76e-54 182
Msa0336680 MtrunA17_Chr8g0357231 32.308 260 171 4 310 566 1 258 8.59e-47 164
Msa0336680 MtrunA17_Chr2g0319071 27.236 492 296 12 90 567 46 489 1.47e-46 170
Msa0336680 MtrunA17_Chr2g0310121 25.709 494 331 12 85 546 271 760 1.41e-38 151
Msa0336680 MtrunA17_Chr2g0319081 30.872 298 184 9 270 561 140 421 4.24e-36 140
Msa0336680 MtrunA17_Chr7g0237071 38.119 202 109 3 86 274 9 207 1.41e-35 133
Msa0336680 MtrunA17_Chr3g0101681 24.851 503 339 13 90 560 277 772 1.76e-35 142
Msa0336680 MtrunA17_Chr6g0466031 25.769 260 188 4 310 566 2 259 1.98e-28 114
Msa0336680 MtrunA17_Chr2g0281651 26.415 318 220 7 245 555 43 353 2.49e-27 114
Msa0336680 MtrunA17_Chr7g0237061 27.801 241 148 6 310 549 2 217 2.88e-27 110
Msa0336680 MtrunA17_Chr8g0357221 33.632 223 140 3 50 264 63 285 1.34e-25 107
Msa0336680 MtrunA17_Chr7g0233861 40.650 123 64 1 220 342 45 158 1.51e-22 95.1
Msa0336680 MtrunA17_Chr6g0466021 36.774 155 84 3 72 215 49 200 2.09e-21 92.4
Msa0336680 MtrunA17_Chr7g0217351 31.163 215 131 6 66 270 66 273 3.48e-19 88.6
Msa0336680 MtrunA17_Chr7g0259881 28.819 288 161 11 92 340 232 514 3.79e-19 91.3
Msa0336680 MtrunA17_Chr7g0218001 27.969 261 151 7 92 317 349 607 7.50e-16 81.3
Msa0336680 MtrunA17_Chr2g0281721 35.000 100 63 2 245 343 29 127 1.16e-12 65.5
Msa0336680 MtrunA17_Chr5g0411111 34.921 126 62 4 130 240 1 121 3.94e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336680 AT4G16730.1 40.467 514 283 7 73 566 28 538 1.97e-130 392
Msa0336680 AT4G16730.2 41.010 495 269 7 92 566 1 492 2.89e-129 387
Msa0336680 AT4G16740.1 36.832 524 300 8 63 566 51 563 1.51e-109 339
Msa0336680 AT1G61680.1 36.345 487 300 6 82 561 86 569 3.79e-104 325
Msa0336680 AT2G24210.1 35.556 495 297 9 90 566 99 589 5.99e-103 323
Msa0336680 AT3G25820.1 34.924 524 305 11 65 566 84 593 1.59e-101 319
Msa0336680 AT3G25830.1 34.924 524 305 11 65 566 84 593 1.59e-101 319
Msa0336680 AT3G25810.1 34.600 500 300 10 90 566 101 596 1.73e-98 311
Msa0336680 AT1G61680.2 35.524 487 297 7 82 561 86 562 5.20e-98 309
Msa0336680 AT5G23960.2 30.962 520 346 7 58 566 27 544 2.85e-85 275
Msa0336680 AT5G23960.1 31.034 522 345 8 58 566 27 546 3.81e-84 272
Msa0336680 AT1G70080.1 32.245 490 304 11 92 560 122 604 1.66e-77 257
Msa0336680 AT4G16740.2 37.901 343 190 5 63 388 51 387 5.30e-71 233
Msa0336680 AT3G25820.2 36.134 357 200 8 65 406 84 427 2.60e-68 228
Msa0336680 AT3G14490.1 30.375 507 333 10 72 565 95 594 5.99e-66 226
Msa0336680 AT3G29410.2 29.915 468 312 7 90 546 13 475 2.21e-64 219
Msa0336680 AT1G33750.1 29.008 524 353 9 54 566 87 602 3.41e-64 221
Msa0336680 AT3G29410.1 29.915 468 312 7 90 546 118 580 1.79e-63 219
Msa0336680 AT1G31950.3 28.143 533 360 9 50 566 81 606 1.95e-61 214
Msa0336680 AT1G31950.1 29.213 534 352 11 50 566 81 605 2.12e-61 214
Msa0336680 AT3G14540.1 30.105 475 311 8 90 549 113 581 4.12e-61 213
Msa0336680 AT4G15870.1 27.829 539 356 13 20 547 60 576 9.92e-60 209
Msa0336680 AT3G14520.1 28.270 474 321 7 90 549 116 584 2.57e-57 202
Msa0336680 AT5G44630.1 30.189 477 304 12 87 546 70 534 2.47e-55 196
Msa0336680 AT3G32030.1 26.419 511 357 7 72 567 98 604 4.74e-55 196
Msa0336680 AT4G16740.4 36.164 318 180 5 63 363 51 362 5.00e-54 188
Msa0336680 AT4G16740.3 36.164 318 180 5 63 363 51 362 5.00e-54 188
Msa0336680 AT4G13300.1 28.376 511 349 9 66 561 40 548 7.13e-54 192
Msa0336680 AT2G23230.2 29.981 527 322 15 66 561 84 594 3.16e-53 191
Msa0336680 AT2G23230.1 30.077 522 323 15 66 561 84 589 6.66e-53 190
Msa0336680 AT1G31950.2 29.493 434 286 7 50 470 81 507 5.44e-52 186
Msa0336680 AT3G29110.1 28.770 504 322 11 67 546 84 574 1.23e-51 187
Msa0336680 AT4G20230.1 27.766 461 321 7 92 542 119 577 2.16e-51 186
Msa0336680 AT4G20230.2 27.766 461 321 7 92 542 131 589 2.29e-51 186
Msa0336680 AT4G13280.2 28.700 446 309 6 123 561 105 548 2.98e-51 185
Msa0336680 AT3G25820.4 36.519 293 158 8 65 342 84 363 1.07e-50 179
Msa0336680 AT3G25820.3 36.519 293 158 8 65 342 84 363 1.07e-50 179
Msa0336680 AT3G14540.2 30.633 395 254 7 90 470 113 501 1.18e-50 182
Msa0336680 AT3G29190.1 29.469 414 282 6 92 496 116 528 1.34e-50 184
Msa0336680 AT5G48110.1 29.375 320 221 4 228 546 2 317 2.13e-50 177
Msa0336680 AT4G20210.1 27.879 495 325 11 92 565 115 598 2.23e-49 181
Msa0336680 AT1G66020.1 29.032 465 310 9 92 542 113 571 1.00e-48 179
Msa0336680 AT4G20200.1 28.448 464 314 6 92 542 119 577 1.07e-48 179
Msa0336680 AT1G48800.1 29.436 479 312 13 90 552 118 586 1.51e-48 178
Msa0336680 AT1G48820.1 26.994 489 311 12 67 547 92 542 6.96e-43 162
Msa0336680 AT1G66020.2 35.714 280 163 6 92 358 113 388 1.83e-42 158
Msa0336680 AT4G13280.1 27.354 446 285 8 123 561 105 518 1.35e-41 157
Msa0336680 AT1G79460.1 25.341 513 340 13 85 562 270 774 6.03e-33 134
Msa0336680 AT1G61120.1 26.478 423 273 7 84 478 280 692 7.23e-31 128
Msa0336680 AT4G02780.1 29.885 261 146 8 92 317 334 592 1.87e-18 89.7

Find 119 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAATATGTCTTAAAATT+TGG 0.090930 2_4:-56150976 None:intergenic
ACACTTGCTCGTTGTAATTT+TGG 0.108263 2_4:+56146514 Msa0336680:CDS
TTATATGAGGCATCATATTT+AGG 0.175329 2_4:+56149559 Msa0336680:CDS
ATTCCAAAAGAGTTGGTATT+TGG 0.197928 2_4:-56149768 None:intergenic
CCGGGGAAATTCTTCGACTA+TGG 0.229539 2_4:+56151124 Msa0336680:CDS
TTTAAGACATATTTGGATAA+TGG 0.251513 2_4:+56150982 Msa0336680:CDS
TAACCAAATACCAACTCTTT+TGG 0.266679 2_4:+56149765 Msa0336680:CDS
TATATGAGGCATCATATTTA+GGG 0.283045 2_4:+56149560 Msa0336680:CDS
ATATTTCTGCCAATTCTCTT+TGG 0.285413 2_4:-56149828 None:intergenic
TGAAGATAAGACCAAAAGTT+TGG 0.285905 2_4:+56147970 Msa0336680:CDS
GCCATTGTCATTGTATTTGC+AGG 0.288475 2_4:-56146457 None:intergenic
ATCACTTACCAATGACATTT+GGG 0.292181 2_4:+56149525 Msa0336680:CDS
GATGATATCTTTGACACTTA+TGG 0.293561 2_4:+56150519 Msa0336680:CDS
TTTGACTTGACCCAAACTTT+TGG 0.296123 2_4:-56147981 None:intergenic
CCAATTTGCCTGCCTACTTC+AGG 0.296197 2_4:-56149661 None:intergenic
TAGCATATCTTCATGTATTC+AGG 0.305968 2_4:-56150711 None:intergenic
CATTTCTTGAAGAAGCTAAA+TGG 0.310663 2_4:+56150935 Msa0336680:CDS
CTTCTAACCTTGCCATTCTT+AGG 0.311069 2_4:-56149708 None:intergenic
CAATTTGAATGACATGGTTC+TGG 0.331181 2_4:-56150453 None:intergenic
TTGCCACATCCCTTCAATTT+CGG 0.335451 2_4:+56148161 Msa0336680:CDS
CTTGCCATTCTTAGGTGCTT+TGG 0.346897 2_4:-56149700 None:intergenic
AATCACTTACCAATGACATT+TGG 0.351614 2_4:+56149524 Msa0336680:CDS
CCTGAAGTAGGCAGGCAAAT+TGG 0.353627 2_4:+56149661 Msa0336680:CDS
ATGAAGGAAGAATACAAATA+AGG 0.356949 2_4:+56151872 Msa0336680:CDS
TCAATCCAAGGGCTGGGAAT+TGG 0.367737 2_4:+56148067 Msa0336680:CDS
CCCAGGCTCTTCACTTGCTC+CGG 0.372004 2_4:+56151105 Msa0336680:CDS
ATACATGAAGATATGCTATA+TGG 0.378469 2_4:+56150716 Msa0336680:CDS
TCTATATGCAATTTCATTTG+TGG 0.378965 2_4:-56150751 None:intergenic
GACCAACGGAGTGCTTCTTA+TGG 0.384882 2_4:+56150415 Msa0336680:CDS
ATTAGGTGGTGGAAAGAGTT+GGG 0.386750 2_4:+56150360 Msa0336680:intron
GGTTACTGAGGCATAATTGC+TGG 0.386853 2_4:+56148182 Msa0336680:CDS
GAAGCTAAATGGTTCAATAA+TGG 0.388219 2_4:+56150946 Msa0336680:CDS
GCTGGGAATTGGTCACCATT+TGG 0.393146 2_4:+56148078 Msa0336680:CDS
GTCATTACACCAAAGAGAAT+TGG 0.404125 2_4:+56149819 Msa0336680:CDS
TTGAATTTAGCAATGCTTCT+CGG 0.408026 2_4:-56148012 None:intergenic
AACTTGGGAGGATATGTGAT+TGG 0.414855 2_4:+56148119 Msa0336680:CDS
ATACAAATAAGGATGCTACT+AGG 0.418565 2_4:+56151883 Msa0336680:CDS
CCATTCTTAGGTGCTTTGGA+AGG 0.418608 2_4:-56149696 None:intergenic
GGGTATGTTGAGTTTATATG+AGG 0.434234 2_4:+56149546 Msa0336680:CDS
ATCATCTTCAATGTTACAAT+AGG 0.441194 2_4:-56148040 None:intergenic
GGATGCTACTAGGGAATTTG+TGG 0.444878 2_4:+56151893 Msa0336680:CDS
GAAGATAAGACCAAAAGTTT+GGG 0.446624 2_4:+56147971 Msa0336680:CDS
ATTCTCTTTGGTGTAATGAC+TGG 0.447027 2_4:-56149816 None:intergenic
ATGCATGCCACCTTCCTCAT+TGG 0.449603 2_4:+56151036 Msa0336680:CDS
TGTGTTATCACAAACATAGA+AGG 0.454963 2_4:+56146423 None:intergenic
TAGGTGCTTTGGAAGGGTTA+AGG 0.456059 2_4:-56149689 None:intergenic
GCCTGCCTACTTCAGGACTA+AGG 0.459721 2_4:-56149654 None:intergenic
CAACATACCCCAAATGTCAT+TGG 0.463701 2_4:-56149533 None:intergenic
CATAACTAGCAAAATACATA+TGG 0.467636 2_4:-56150496 None:intergenic
CCAGGCTCTTCACTTGCTCC+GGG 0.468250 2_4:+56151106 Msa0336680:CDS
GGTGACCAATTCCCAGCCCT+TGG 0.469761 2_4:-56148072 None:intergenic
GATAATGGAGTGATTTCTGC+AGG 0.473286 2_4:+56150997 Msa0336680:CDS
TTGGGACTTGTTGACAAACT+TGG 0.473750 2_4:+56150378 Msa0336680:CDS
GATTGACTCAATCCAAGGGC+TGG 0.474173 2_4:+56148060 Msa0336680:CDS
GTCTTATCTTCAGTTTGTAA+AGG 0.481471 2_4:-56147959 None:intergenic
GATTAGGTGGTGGAAAGAGT+TGG 0.483866 2_4:+56150359 Msa0336680:intron
TACAAATAAGGATGCTACTA+GGG 0.488175 2_4:+56151884 Msa0336680:CDS
CTTCGACTATGGGATGACTT+AGG 0.488396 2_4:+56151135 Msa0336680:CDS
CGGGGAAATTCTTCGACTAT+GGG 0.489879 2_4:+56151125 Msa0336680:CDS
AATTGCTGGCCCACATGTCC+TGG 0.494407 2_4:+56148196 Msa0336680:CDS
CATTGATCTCATCTTCCAAA+TGG 0.495809 2_4:-56148093 None:intergenic
ACTTGGGAGGATATGTGATT+GGG 0.499523 2_4:+56148120 Msa0336680:CDS
GCCACCTTCCTCATTGGTGA+TGG 0.504456 2_4:+56151042 Msa0336680:CDS
CATTCTTAGGTGCTTTGGAA+GGG 0.508082 2_4:-56149695 None:intergenic
TTTGCCAATTCCAAAAGAGT+TGG 0.511202 2_4:-56149775 None:intergenic
GCCTCAGTAACCGAAATTGA+AGG 0.515765 2_4:-56148171 None:intergenic
AATATTTCAGATGAAAATGA+AGG 0.518222 2_4:+56151856 Msa0336680:CDS
ATTGACTCAATCCAAGGGCT+GGG 0.520399 2_4:+56148061 Msa0336680:CDS
GATTTCTGCAGGATCATGTA+TGG 0.522815 2_4:+56151008 Msa0336680:CDS
GAGTGCTTCTTATGGACAGT+AGG 0.523662 2_4:+56150423 Msa0336680:CDS
CCTTCCAAAGCACCTAAGAA+TGG 0.525082 2_4:+56149696 Msa0336680:CDS
GGGATATGACTTCATCATCA+TGG 0.527240 2_4:-56151085 None:intergenic
ACCTTAGTCCTGAAGTAGGC+AGG 0.530056 2_4:+56149653 Msa0336680:CDS
GAGAATTGGCAGAAATATGC+AGG 0.535303 2_4:+56149833 Msa0336680:CDS
CAGATGGGACCTTGATGCAA+TGG 0.538910 2_4:+56150680 Msa0336680:intron
CCCTTCAATTTCGGTTACTG+AGG 0.554070 2_4:+56148170 Msa0336680:CDS
ACGAGCCCTTGAAAATTCCA+TGG 0.555738 2_4:-56149611 None:intergenic
GTTCAGGAGGAACAAGAAAG+AGG 0.555777 2_4:+56151793 Msa0336680:intron
CCCGGAGCAAGTGAAGAGCC+TGG 0.558035 2_4:-56151106 None:intergenic
TAACCGAAATTGAAGGGATG+TGG 0.558085 2_4:-56148164 None:intergenic
AGGTAATGATGTGAAGATCA+AGG 0.564747 2_4:-56152058 None:intergenic
GATGAGATCAATGTTCAACT+TGG 0.572324 2_4:+56148103 Msa0336680:CDS
ATGCGGCAATTTGAATGACA+TGG 0.572463 2_4:-56150459 None:intergenic
AAGATGATTGACTCAATCCA+AGG 0.578634 2_4:+56148055 Msa0336680:CDS
GTCCATTGCTCTCAATGAGA+AGG 0.579260 2_4:+56146615 Msa0336680:CDS
CCACACCTTAGTCCTGAAGT+AGG 0.581171 2_4:+56149649 Msa0336680:CDS
CCTCAGTAACCGAAATTGAA+GGG 0.583119 2_4:-56148170 None:intergenic
CCGGAGCAAGTGAAGAGCCT+GGG 0.583946 2_4:-56151105 None:intergenic
TGATGATGAAGTCATATCCC+AGG 0.589646 2_4:+56151088 Msa0336680:CDS
CTACTTCAGGACTAAGGTGT+GGG 0.590174 2_4:-56149648 None:intergenic
TACCTTCTCATTGAGAGCAA+TGG 0.593656 2_4:-56146617 None:intergenic
TGCAATATACCAGGACATGT+GGG 0.593982 2_4:-56148205 None:intergenic
CATTGGTGATGGTCTCTCAA+AGG 0.594573 2_4:+56151053 Msa0336680:CDS
ATGAGATCAATGTTCAACTT+GGG 0.595916 2_4:+56148104 Msa0336680:CDS
GAAGTTGCTCCATTGCATCA+AGG 0.597358 2_4:-56150689 None:intergenic
TATCAAAGCTTCTCTTAACA+TGG 0.598838 2_4:+56151963 Msa0336680:CDS
ATGCAATATACCAGGACATG+TGG 0.603297 2_4:-56148206 None:intergenic
CCATAGTCGAAGAATTTCCC+CGG 0.608178 2_4:-56151124 None:intergenic
GTCCATAAGAAGCACTCCGT+TGG 0.609668 2_4:-56150417 None:intergenic
TCAAATTCAAGGATTATGCA+TGG 0.614482 2_4:+56146577 Msa0336680:CDS
AGTGCTTCTTATGGACAGTA+GGG 0.615566 2_4:+56150424 Msa0336680:CDS
AGATCAATGTTCAACTTGGG+AGG 0.619212 2_4:+56148107 Msa0336680:CDS
GTGGCAAAGAGGTCTTGAGA+AGG 0.619717 2_4:-56148145 None:intergenic
GCTCAAGTTATTTATCAACA+TGG 0.621098 2_4:+56151994 Msa0336680:CDS
GAGACCATCACCAATGAGGA+AGG 0.621909 2_4:-56151046 None:intergenic
CCTACTTCAGGACTAAGGTG+TGG 0.623254 2_4:-56149649 None:intergenic
CAAAGCACCTAAGAATGGCA+AGG 0.625330 2_4:+56149701 Msa0336680:CDS
TGGCAAAGAGGTCTTGAGAA+GGG 0.625651 2_4:-56148144 None:intergenic
GCCTGCAAATACAATGACAA+TGG 0.627016 2_4:+56146456 Msa0336680:exon
TATAGAATTCAGAAAGAACA+TGG 0.628836 2_4:+56150768 Msa0336680:CDS
GGATGACTTAGGAACTTCAA+CGG 0.635407 2_4:+56151146 Msa0336680:CDS
TTGAGAGACCATCACCAATG+AGG 0.655213 2_4:-56151050 None:intergenic
AGATGATTGACTCAATCCAA+GGG 0.655644 2_4:+56148056 Msa0336680:CDS
ATTGAAGGGATGTGGCAAAG+AGG 0.660331 2_4:-56148156 None:intergenic
TCACTTACCAATGACATTTG+GGG 0.667503 2_4:+56149526 Msa0336680:CDS
ACCATCACCAATGAGGAAGG+TGG 0.672270 2_4:-56151043 None:intergenic
CAGGCTCTTCACTTGCTCCG+GGG 0.678340 2_4:+56151107 Msa0336680:CDS
AATTTGTGGAGAGAACTCAA+TGG 0.695889 2_4:+56151907 Msa0336680:CDS
GAAGCTTTGATAACAGACGA+AGG 0.721487 2_4:-56151952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTAATAAATAAA+GGG + chr2_4:56151189-56151208 Msa0336680:intron 0.0%
!! AAATTAGTTACTATAATTTA+GGG - chr2_4:56147498-56147517 None:intergenic 10.0%
!! AAAATTAGTTACTATAATTT+AGG - chr2_4:56147499-56147518 None:intergenic 10.0%
!!! AATCAGTTTTTAAAATAATA+TGG + chr2_4:56147832-56147851 Msa0336680:intron 10.0%
!! ACTCAATAAAAAAATAAATT+TGG - chr2_4:56148366-56148385 None:intergenic 10.0%
!! TTAAATATACATTATATAAG+AGG + chr2_4:56148529-56148548 Msa0336680:intron 10.0%
!!! TTTAATTAAATATTTTGTTG+TGG - chr2_4:56149880-56149899 None:intergenic 10.0%
!! ACAACAAAATATTTAATTAA+AGG + chr2_4:56149879-56149898 Msa0336680:intron 10.0%
!! AAAATTTCATCTTTAAATAT+CGG - chr2_4:56150163-56150182 None:intergenic 10.0%
!!! TTATTAATTAATTTTCAGTA+GGG - chr2_4:56151184-56151203 None:intergenic 10.0%
!!! TTTATTAATTAATTTTCAGT+AGG - chr2_4:56151185-56151204 None:intergenic 10.0%
!!! CAAATTTAAATTTTTATTAG+TGG + chr2_4:56151586-56151605 Msa0336680:intron 10.0%
!!! TTTTTCAAATAAAATACTAT+AGG - chr2_4:56152119-56152138 None:intergenic 10.0%
!!! TAGTATTTTATTTGAAAAAT+AGG + chr2_4:56152120-56152139 Msa0336680:three_prime_UTR 10.0%
!!! AGTATTTTATTTGAAAAATA+GGG + chr2_4:56152121-56152140 Msa0336680:three_prime_UTR 10.0%
!!! ATTGATGATTATATAAGATA+GGG - chr2_4:56146661-56146680 None:intergenic 15.0%
!!! TATTGATGATTATATAAGAT+AGG - chr2_4:56146662-56146681 None:intergenic 15.0%
!! TAAATTTAAATTCATTGTTG+TGG + chr2_4:56146896-56146915 Msa0336680:intron 15.0%
!! TTATTTGTAAAATTGTTTGA+AGG + chr2_4:56147204-56147223 Msa0336680:intron 15.0%
!!! AATTATAGTAACTAATTTTG+CGG + chr2_4:56147500-56147519 Msa0336680:intron 15.0%
!! AAATAAAAAGATGTGATTTA+CGG - chr2_4:56147790-56147809 None:intergenic 15.0%
!!! TCTTTTTATTTAGAAGTAAA+TGG + chr2_4:56147799-56147818 Msa0336680:intron 15.0%
!!! TCTAAGACAATATTTTATAA+GGG - chr2_4:56147880-56147899 None:intergenic 15.0%
!!! TTCTAAGACAATATTTTATA+AGG - chr2_4:56147881-56147900 None:intergenic 15.0%
!! AGAAATAAAAAGAATGAATT+TGG - chr2_4:56148476-56148495 None:intergenic 15.0%
!!! TTTTTATTTCTAACTTTAGT+GGG + chr2_4:56148485-56148504 Msa0336680:intron 15.0%
!!! CATTATATTTTTTTTTGACA+AGG + chr2_4:56149179-56149198 Msa0336680:intron 15.0%
!! ACAAAATATTTAATTAAAGG+TGG + chr2_4:56149882-56149901 Msa0336680:intron 15.0%
!!! ATTCAATATTGATTTAGATT+TGG - chr2_4:56150106-56150125 None:intergenic 15.0%
!!! CAAATTTAAATTTTTATCAG+TGG + chr2_4:56151331-56151350 Msa0336680:intron 15.0%
!!! AATATTTTTATTAGTGGTAA+AGG + chr2_4:56151397-56151416 Msa0336680:intron 15.0%
!!! TTTATTACAATTTGTTTCAT+AGG - chr2_4:56151727-56151746 None:intergenic 15.0%
!!! ATCATTTTTTTTTTTGTTGA+AGG + chr2_4:56152220-56152239 Msa0336680:three_prime_UTR 15.0%
!!! TAATCATCAATATGAAGTTT+TGG + chr2_4:56146670-56146689 Msa0336680:intron 20.0%
!!! AATCATCAATATGAAGTTTT+GGG + chr2_4:56146671-56146690 Msa0336680:intron 20.0%
!!! ACTTGTCAAAATCAATTTTA+AGG - chr2_4:56146780-56146799 None:intergenic 20.0%
!!! ATATCAAACTGATTTATGTT+TGG - chr2_4:56146990-56147009 None:intergenic 20.0%
!! AAGTTGAATATAAAAGTTCT+AGG + chr2_4:56147041-56147060 Msa0336680:intron 20.0%
!! AGCATCTTCTTAAAAAATAA+AGG + chr2_4:56147103-56147122 Msa0336680:intron 20.0%
!! GCATCTTCTTAAAAAATAAA+GGG + chr2_4:56147104-56147123 Msa0336680:intron 20.0%
!! TAAATCTTATGTAGAAATGA+AGG + chr2_4:56147149-56147168 Msa0336680:intron 20.0%
!!! TGTTTGTTTTAAGATGTTAT+TGG + chr2_4:56147702-56147721 Msa0336680:intron 20.0%
!!! GTTTGTTTTAAGATGTTATT+GGG + chr2_4:56147703-56147722 Msa0336680:intron 20.0%
!!! CTTTTTATTTCTAACTTTAG+TGG + chr2_4:56148484-56148503 Msa0336680:intron 20.0%
!! TTAAGAAAATGTTTCTTCTA+TGG - chr2_4:56148840-56148859 None:intergenic 20.0%
!!! ATTTTCACCATTAAACTATT+TGG - chr2_4:56148891-56148910 None:intergenic 20.0%
!! TGTAAGTATAATTCACTTAA+TGG - chr2_4:56148989-56149008 None:intergenic 20.0%
!! AATGTATTACTACTATATTC+AGG + chr2_4:56149344-56149363 Msa0336680:intron 20.0%
!!! TTTATAAGTATTGTATAAGC+AGG + chr2_4:56149367-56149386 Msa0336680:intron 20.0%
!! AGAAATATTAAAAAAAGCCA+TGG + chr2_4:56149594-56149613 Msa0336680:CDS 20.0%
!!! CTAGTTTATTTGATTTGATT+AGG + chr2_4:56150343-56150362 Msa0336680:intron 20.0%
!!! TAAACTTTATGTTTTACAGA+TGG + chr2_4:56150664-56150683 Msa0336680:intron 20.0%
!!! AAACTTTATGTTTTACAGAT+GGG + chr2_4:56150665-56150684 Msa0336680:intron 20.0%
!! TCCAAATATGTCTTAAAATT+TGG - chr2_4:56150979-56150998 None:intergenic 20.0%
!!! TTTAAGACATATTTGGATAA+TGG + chr2_4:56150982-56151001 Msa0336680:CDS 20.0%
!! TATAAATTTGTATCTACTCA+AGG - chr2_4:56151637-56151656 None:intergenic 20.0%
!!! TCTTACTAATTTTTATGTTC+AGG + chr2_4:56151777-56151796 Msa0336680:intron 20.0%
!! AATATTTCAGATGAAAATGA+AGG + chr2_4:56151856-56151875 Msa0336680:CDS 20.0%
!!! TAAATGACATATTTTTGTCA+TGG + chr2_4:56152197-56152216 Msa0336680:three_prime_UTR 20.0%
!!! AGTGTGTTTGTTTTTACTAT+AGG + chr2_4:56146734-56146753 Msa0336680:intron 25.0%
!!! GTGTGTTTGTTTTTACTATA+GGG + chr2_4:56146735-56146754 Msa0336680:intron 25.0%
!!! GTCAAAATCAATTTTAAGGT+TGG - chr2_4:56146776-56146795 None:intergenic 25.0%
!!! AATTGATTTTGACAAGTTTG+TGG + chr2_4:56146783-56146802 Msa0336680:intron 25.0%
! AATTGCAAGTGATTTATGTT+TGG - chr2_4:56146938-56146957 None:intergenic 25.0%
! CAAATAACACACTGTATAAT+TGG - chr2_4:56147191-56147210 None:intergenic 25.0%
! AATTTAGGGAAACTTGTATA+TGG - chr2_4:56147484-56147503 None:intergenic 25.0%
! ATCATCTTCAATGTTACAAT+AGG - chr2_4:56148043-56148062 None:intergenic 25.0%
!! CTTTGAATTTTCTTATGCTT+TGG + chr2_4:56148240-56148259 Msa0336680:intron 25.0%
! CAATCAAAACATAATAGCAT+AGG + chr2_4:56148293-56148312 Msa0336680:intron 25.0%
!!! AAATCAGAGTTTTGATTTTG+TGG + chr2_4:56148554-56148573 Msa0336680:intron 25.0%
!!! AATCAGAGTTTTGATTTTGT+GGG + chr2_4:56148555-56148574 Msa0336680:intron 25.0%
! TCATTGGGAATATTTGTATA+GGG - chr2_4:56148794-56148813 None:intergenic 25.0%
! TTCATTGGGAATATTTGTAT+AGG - chr2_4:56148795-56148814 None:intergenic 25.0%
! CTGTATATGTTTGTTTCATT+GGG - chr2_4:56148809-56148828 None:intergenic 25.0%
! ACTGTATATGTTTGTTTCAT+TGG - chr2_4:56148810-56148829 None:intergenic 25.0%
! TTTGTTGCCAAATAGTTTAA+TGG + chr2_4:56148881-56148900 Msa0336680:intron 25.0%
!! TCTACACTTTTCTATATTCA+GGG - chr2_4:56148920-56148939 None:intergenic 25.0%
!! TTCTACACTTTTCTATATTC+AGG - chr2_4:56148921-56148940 None:intergenic 25.0%
! TCATGGATATTGAATATGTT+GGG - chr2_4:56148964-56148983 None:intergenic 25.0%
! TTCATGGATATTGAATATGT+TGG - chr2_4:56148965-56148984 None:intergenic 25.0%
!!! TTCTACTAAGTCTATTTTGA+TGG - chr2_4:56149081-56149100 None:intergenic 25.0%
! AAATAGACTTAGTAGAAATC+AGG + chr2_4:56149084-56149103 Msa0336680:intron 25.0%
!!! CTAGTTTTTTTACTGAAAGA+AGG - chr2_4:56149253-56149272 None:intergenic 25.0%
!!! AGATGTTTTCAGGAATTTTT+TGG + chr2_4:56149480-56149499 Msa0336680:intron 25.0%
!! TTATATGAGGCATCATATTT+AGG + chr2_4:56149559-56149578 Msa0336680:CDS 25.0%
!! TATATGAGGCATCATATTTA+GGG + chr2_4:56149560-56149579 Msa0336680:CDS 25.0%
! TCATAATTTCACACATTCAT+CGG + chr2_4:56149931-56149950 Msa0336680:intron 25.0%
!!! CGGTTGCTAATTTAATTTTT+TGG - chr2_4:56150143-56150162 None:intergenic 25.0%
! TTCATGGCAGAAATTAATAT+TGG - chr2_4:56150260-56150279 None:intergenic 25.0%
!!! GTTTATTTGATTTGATTAGG+TGG + chr2_4:56150346-56150365 Msa0336680:intron 25.0%
! CATAACTAGCAAAATACATA+TGG - chr2_4:56150499-56150518 None:intergenic 25.0%
!! CATATGTATTTTGCTAGTTA+TGG + chr2_4:56150497-56150516 Msa0336680:CDS 25.0%
!! ACATAAAGTTTAGTTTAGCA+TGG - chr2_4:56150656-56150675 None:intergenic 25.0%
! ATACATGAAGATATGCTATA+TGG + chr2_4:56150716-56150735 Msa0336680:CDS 25.0%
! TCTATATGCAATTTCATTTG+TGG - chr2_4:56150754-56150773 None:intergenic 25.0%
! TATAGAATTCAGAAAGAACA+TGG + chr2_4:56150768-56150787 Msa0336680:CDS 25.0%
! ATTATTAACATGTCACTCTT+TGG - chr2_4:56150869-56150888 None:intergenic 25.0%
! CATGTTAATAATTGTAGACT+TGG + chr2_4:56150877-56150896 Msa0336680:intron 25.0%
!!! GCCAAATTTTAAGACATATT+TGG + chr2_4:56150975-56150994 Msa0336680:CDS 25.0%
!! AATTTGTTTCATAGGAATCA+TGG - chr2_4:56151719-56151738 None:intergenic 25.0%
!! TACTAATTTTTATGTTCAGG+AGG + chr2_4:56151780-56151799 Msa0336680:intron 25.0%
! ATGAAGGAAGAATACAAATA+AGG + chr2_4:56151872-56151891 Msa0336680:CDS 25.0%
!!! TCTTCAACTCAATTTTTAGT+AGG - chr2_4:56152081-56152100 None:intergenic 25.0%
CGCTACAAAAATCAAATTCA+AGG + chr2_4:56146566-56146585 Msa0336680:CDS 30.0%
TCAAATTCAAGGATTATGCA+TGG + chr2_4:56146577-56146596 Msa0336680:CDS 30.0%
CTTGCAATTCAATCAATTCA+TGG + chr2_4:56146949-56146968 Msa0336680:intron 30.0%
CAATTCAATCAATTCATGGT+TGG + chr2_4:56146953-56146972 Msa0336680:intron 30.0%
AAAATAAAGGGTTATGCTAG+AGG + chr2_4:56147116-56147135 Msa0336680:intron 30.0%
! TTTTGAACTCAACTCTTACA+CGG - chr2_4:56147555-56147574 None:intergenic 30.0%
GTCTTATCTTCAGTTTGTAA+AGG - chr2_4:56147962-56147981 None:intergenic 30.0%
TGAAGATAAGACCAAAAGTT+TGG + chr2_4:56147970-56147989 Msa0336680:CDS 30.0%
GAAGATAAGACCAAAAGTTT+GGG + chr2_4:56147971-56147990 Msa0336680:CDS 30.0%
TTGAATTTAGCAATGCTTCT+CGG - chr2_4:56148015-56148034 None:intergenic 30.0%
ATGAGATCAATGTTCAACTT+GGG + chr2_4:56148104-56148123 Msa0336680:CDS 30.0%
ATACATGAATGCAATATACC+AGG - chr2_4:56148217-56148236 None:intergenic 30.0%
AGAGGTCTAGGTTTATATAT+GGG - chr2_4:56148433-56148452 None:intergenic 30.0%
TAGAGGTCTAGGTTTATATA+TGG - chr2_4:56148434-56148453 None:intergenic 30.0%
!!! ATCAGAGTTTTGATTTTGTG+GGG + chr2_4:56148556-56148575 Msa0336680:intron 30.0%
! CACTTAAGTGATATACCTTT+TGG - chr2_4:56148589-56148608 None:intergenic 30.0%
ATATCACTTAAGTGAGTGTT+TGG + chr2_4:56148595-56148614 Msa0336680:intron 30.0%
!! TAATGACTTGTTTGGTTATG+CGG - chr2_4:56148740-56148759 None:intergenic 30.0%
!!! GTTTTGACTAATGACTTGTT+TGG - chr2_4:56148748-56148767 None:intergenic 30.0%
ATTGGGAATATTTGTATAGG+GGG - chr2_4:56148792-56148811 None:intergenic 30.0%
CATTGGGAATATTTGTATAG+GGG - chr2_4:56148793-56148812 None:intergenic 30.0%
CATGGATATTGAATATGTTG+GGG - chr2_4:56148963-56148982 None:intergenic 30.0%
TAATTCACTTAATGGCTTCA+TGG - chr2_4:56148981-56149000 None:intergenic 30.0%
TACATGACATAATGACCTTA+TGG + chr2_4:56149005-56149024 Msa0336680:intron 30.0%
!! ATTTTGATGGCTAAATGCAA+GGG - chr2_4:56149068-56149087 None:intergenic 30.0%
!!! TATTTTGATGGCTAAATGCA+AGG - chr2_4:56149069-56149088 None:intergenic 30.0%
!! TTTTACTGAAAGAAGGTACA+AGG - chr2_4:56149246-56149265 None:intergenic 30.0%
CCAATAATACATGTCAGTAT+AGG - chr2_4:56149308-56149327 None:intergenic 30.0%
CCTATACTGACATGTATTAT+TGG + chr2_4:56149305-56149324 Msa0336680:intron 30.0%
! ACTTGATGATTTGATTAGTG+TGG + chr2_4:56149445-56149464 Msa0336680:intron 30.0%
ATTTGATTAGTGTGGTTGTT+TGG + chr2_4:56149453-56149472 Msa0336680:intron 30.0%
AATCACTTACCAATGACATT+TGG + chr2_4:56149524-56149543 Msa0336680:CDS 30.0%
ATCACTTACCAATGACATTT+GGG + chr2_4:56149525-56149544 Msa0336680:CDS 30.0%
! AAAAAGCCATGGAATTTTCA+AGG + chr2_4:56149605-56149624 Msa0336680:CDS 30.0%
! AAAAGCCATGGAATTTTCAA+GGG + chr2_4:56149606-56149625 Msa0336680:CDS 30.0%
! TAACCAAATACCAACTCTTT+TGG + chr2_4:56149765-56149784 Msa0336680:CDS 30.0%
!! ATTCCAAAAGAGTTGGTATT+TGG - chr2_4:56149771-56149790 None:intergenic 30.0%
!!! AATACCAACTCTTTTGGAAT+TGG + chr2_4:56149771-56149790 Msa0336680:CDS 30.0%
! ATATTTCTGCCAATTCTCTT+TGG - chr2_4:56149831-56149850 None:intergenic 30.0%
AAAGGTGGAAGAAATTGATT+TGG + chr2_4:56149897-56149916 Msa0336680:intron 30.0%
! AGTAGACAAAAAGAAGTATG+AGG - chr2_4:56150292-56150311 None:intergenic 30.0%
!! TATTTGATTTGATTAGGTGG+TGG + chr2_4:56150349-56150368 Msa0336680:intron 30.0%
! TGGTTTTTGTAAGTTCAATG+CGG - chr2_4:56150479-56150498 None:intergenic 30.0%
GATGATATCTTTGACACTTA+TGG + chr2_4:56150519-56150538 Msa0336680:CDS 30.0%
!! TGAACTTGTTCTTTTCACAA+AGG + chr2_4:56150551-56150570 Msa0336680:CDS 30.0%
! TTTTCACAAAGGCAATCAAA+AGG + chr2_4:56150562-56150581 Msa0336680:CDS 30.0%
TAGCATATCTTCATGTATTC+AGG - chr2_4:56150714-56150733 None:intergenic 30.0%
! AGATTCTTACTGTTCTTTTC+AGG - chr2_4:56150812-56150831 None:intergenic 30.0%
CATTTCTTGAAGAAGCTAAA+TGG + chr2_4:56150935-56150954 Msa0336680:CDS 30.0%
GAAGCTAAATGGTTCAATAA+TGG + chr2_4:56150946-56150965 Msa0336680:CDS 30.0%
ATTCTTGTAGACATCATCAA+AGG + chr2_4:56151493-56151512 Msa0336680:intron 30.0%
ATACAAATAAGGATGCTACT+AGG + chr2_4:56151883-56151902 Msa0336680:CDS 30.0%
TACAAATAAGGATGCTACTA+GGG + chr2_4:56151884-56151903 Msa0336680:CDS 30.0%
TATCAAAGCTTCTCTTAACA+TGG + chr2_4:56151963-56151982 Msa0336680:CDS 30.0%
GCTCAAGTTATTTATCAACA+TGG + chr2_4:56151994-56152013 Msa0336680:CDS 30.0%
! ACACTTGCTCGTTGTAATTT+TGG + chr2_4:56146514-56146533 Msa0336680:CDS 35.0%
! CACTTGCTCGTTGTAATTTT+GGG + chr2_4:56146515-56146534 Msa0336680:CDS 35.0%
AGTTTGTGGAATGTTGTAGT+TGG + chr2_4:56146797-56146816 Msa0336680:intron 35.0%
AGAAGATGCTAAAAGACACT+TGG - chr2_4:56147093-56147112 None:intergenic 35.0%
TGAGACAAAAAGATGTGTTG+TGG - chr2_4:56147289-56147308 None:intergenic 35.0%
GTGTGAGTGCTACATTTAAT+TGG + chr2_4:56147528-56147547 Msa0336680:intron 35.0%
TAGTAAAACAAAACCAAGCC+AGG - chr2_4:56147609-56147628 None:intergenic 35.0%
! ATTTTCTCCACATGTGTTGT+AGG - chr2_4:56147644-56147663 None:intergenic 35.0%
!!! TTTGACTTGACCCAAACTTT+TGG - chr2_4:56147984-56148003 None:intergenic 35.0%
! AAGATGATTGACTCAATCCA+AGG + chr2_4:56148055-56148074 Msa0336680:CDS 35.0%
! AGATGATTGACTCAATCCAA+GGG + chr2_4:56148056-56148075 Msa0336680:CDS 35.0%
CATTGATCTCATCTTCCAAA+TGG - chr2_4:56148096-56148115 None:intergenic 35.0%
GATGAGATCAATGTTCAACT+TGG + chr2_4:56148103-56148122 Msa0336680:CDS 35.0%
ACTCTCTTGACATGACAAAA+TGG - chr2_4:56148267-56148286 None:intergenic 35.0%
GTTCTAATATGTAGAGGTCT+AGG - chr2_4:56148445-56148464 None:intergenic 35.0%
!!! TCAGAGTTTTGATTTTGTGG+GGG + chr2_4:56148557-56148576 Msa0336680:intron 35.0%
! TGAGTGTTTGGATCAACTTT+TGG + chr2_4:56148607-56148626 Msa0336680:intron 35.0%
TTCCACGTTGAAATTGCATT+GGG - chr2_4:56148649-56148668 None:intergenic 35.0%
AGCTCATATTTGTAGCTTCA+TGG - chr2_4:56148672-56148691 None:intergenic 35.0%
AGCTTCATCTTGTATCCATA+AGG - chr2_4:56149023-56149042 None:intergenic 35.0%
AATACATGTCAGTATAGGTG+TGG - chr2_4:56149303-56149322 None:intergenic 35.0%
! GTTTGGTTGTAGATGTTTTC+AGG + chr2_4:56149470-56149489 Msa0336680:intron 35.0%
!!! AGGAATTTTTTGGACAAGAG+TGG + chr2_4:56149490-56149509 Msa0336680:CDS 35.0%
TCACTTACCAATGACATTTG+GGG + chr2_4:56149526-56149545 Msa0336680:CDS 35.0%
GGGTATGTTGAGTTTATATG+AGG + chr2_4:56149546-56149565 Msa0336680:CDS 35.0%
TTTGCCAATTCCAAAAGAGT+TGG - chr2_4:56149778-56149797 None:intergenic 35.0%
! ATTCTCTTTGGTGTAATGAC+TGG - chr2_4:56149819-56149838 None:intergenic 35.0%
GTCATTACACCAAAGAGAAT+TGG + chr2_4:56149819-56149838 Msa0336680:CDS 35.0%
ACACATTCATCGGTAGTAAT+AGG + chr2_4:56149941-56149960 Msa0336680:intron 35.0%
TTTGTCTATTCTCGATCCAA+TGG + chr2_4:56150042-56150061 Msa0336680:intron 35.0%
ATAAATGTCACGCATTCACA+TGG - chr2_4:56150076-56150095 None:intergenic 35.0%
TTCCTTGTCGCTAAAATTAG+TGG - chr2_4:56150207-56150226 None:intergenic 35.0%
TATGAGGTTTGTGATGTTCA+TGG - chr2_4:56150276-56150295 None:intergenic 35.0%
! TTTTGCAAGAGATAGACCAA+CGG + chr2_4:56150401-56150420 Msa0336680:CDS 35.0%
CAATTTGAATGACATGGTTC+TGG - chr2_4:56150456-56150475 None:intergenic 35.0%
!!! TCTTTTCAGGTGAGAAACAA+CGG - chr2_4:56150799-56150818 None:intergenic 35.0%
TGTCACTCTTTGGTATACTT+TGG - chr2_4:56150859-56150878 None:intergenic 35.0%
!! TTGGTGATTAATTTGTGCAG+TGG + chr2_4:56150896-56150915 Msa0336680:intron 35.0%
AATTTGTGGAGAGAACTCAA+TGG + chr2_4:56151907-56151926 Msa0336680:CDS 35.0%
!! AACAGACGAAGGTATGTTTT+TGG - chr2_4:56151944-56151963 None:intergenic 35.0%
AGGTAATGATGTGAAGATCA+AGG - chr2_4:56152061-56152080 None:intergenic 35.0%
GCCTGCAAATACAATGACAA+TGG + chr2_4:56146456-56146475 Msa0336680:exon 40.0%
GCCATTGTCATTGTATTTGC+AGG - chr2_4:56146460-56146479 None:intergenic 40.0%
TACCTTCTCATTGAGAGCAA+TGG - chr2_4:56146620-56146639 None:intergenic 40.0%
GATGCTAAAAGACACTTGGT+AGG - chr2_4:56147089-56147108 None:intergenic 40.0%
! AGTAACTAATTTTGCGGCCT+AGG + chr2_4:56147506-56147525 Msa0336680:intron 40.0%
TAAATGTAGCACTCACACCT+AGG - chr2_4:56147526-56147545 None:intergenic 40.0%
TAAAACAAAACCAAGCCAGG+TGG - chr2_4:56147606-56147625 None:intergenic 40.0%
GAGAATTCCTACAACACATG+TGG + chr2_4:56147634-56147653 Msa0336680:intron 40.0%
AGATCAATGTTCAACTTGGG+AGG + chr2_4:56148107-56148126 Msa0336680:CDS 40.0%
AACTTGGGAGGATATGTGAT+TGG + chr2_4:56148119-56148138 Msa0336680:CDS 40.0%
ACTTGGGAGGATATGTGATT+GGG + chr2_4:56148120-56148139 Msa0336680:CDS 40.0%
TTGCCACATCCCTTCAATTT+CGG + chr2_4:56148161-56148180 Msa0336680:CDS 40.0%
TAACCGAAATTGAAGGGATG+TGG - chr2_4:56148167-56148186 None:intergenic 40.0%
CCTCAGTAACCGAAATTGAA+GGG - chr2_4:56148173-56148192 None:intergenic 40.0%
TGCAATATACCAGGACATGT+GGG - chr2_4:56148208-56148227 None:intergenic 40.0%
ATGCAATATACCAGGACATG+TGG - chr2_4:56148209-56148228 None:intergenic 40.0%
GACCTCTACATATTAGAACG+TGG + chr2_4:56148446-56148465 Msa0336680:intron 40.0%
CTCCACGTTCTAATATGTAG+AGG - chr2_4:56148451-56148470 None:intergenic 40.0%
GTTCCACGTTGAAATTGCAT+TGG - chr2_4:56148650-56148669 None:intergenic 40.0%
TGGATACAAGATGAAGCTCT+AGG + chr2_4:56149025-56149044 Msa0336680:intron 40.0%
AATGCAAGGGTGGAAACATT+GGG - chr2_4:56149055-56149074 None:intergenic 40.0%
AAATGCAAGGGTGGAAACAT+TGG - chr2_4:56149056-56149075 None:intergenic 40.0%
! TGGACAAGAGTGGCAATTTT+AGG + chr2_4:56149500-56149519 Msa0336680:CDS 40.0%
! GGACAAGAGTGGCAATTTTA+GGG + chr2_4:56149501-56149520 Msa0336680:CDS 40.0%
CAACATACCCCAAATGTCAT+TGG - chr2_4:56149536-56149555 None:intergenic 40.0%
!! CATTCTTAGGTGCTTTGGAA+GGG - chr2_4:56149698-56149717 None:intergenic 40.0%
CTTCTAACCTTGCCATTCTT+AGG - chr2_4:56149711-56149730 None:intergenic 40.0%
GAGAATTGGCAGAAATATGC+AGG + chr2_4:56149833-56149852 Msa0336680:CDS 40.0%
!! TGCCACTAATTTTAGCGACA+AGG + chr2_4:56150202-56150221 Msa0336680:intron 40.0%
!! ATTAGGTGGTGGAAAGAGTT+GGG + chr2_4:56150360-56150379 Msa0336680:intron 40.0%
!! TTGGGACTTGTTGACAAACT+TGG + chr2_4:56150378-56150397 Msa0336680:CDS 40.0%
AGTGCTTCTTATGGACAGTA+GGG + chr2_4:56150424-56150443 Msa0336680:CDS 40.0%
ATGCGGCAATTTGAATGACA+TGG - chr2_4:56150462-56150481 None:intergenic 40.0%
CACAAAGGCAATCAAAAGGT+TGG + chr2_4:56150566-56150585 Msa0336680:intron 40.0%
!! GATAATGGAGTGATTTCTGC+AGG + chr2_4:56150997-56151016 Msa0336680:CDS 40.0%
GATTTCTGCAGGATCATGTA+TGG + chr2_4:56151008-56151027 Msa0336680:CDS 40.0%
GGGATATGACTTCATCATCA+TGG - chr2_4:56151088-56151107 None:intergenic 40.0%
! TGATGATGAAGTCATATCCC+AGG + chr2_4:56151088-56151107 Msa0336680:CDS 40.0%
GGATGACTTAGGAACTTCAA+CGG + chr2_4:56151146-56151165 Msa0336680:CDS 40.0%
!!! CGAAGGTATGTTTTTGGTCA+TGG - chr2_4:56151938-56151957 None:intergenic 40.0%
! GAAGCTTTGATAACAGACGA+AGG - chr2_4:56151955-56151974 None:intergenic 40.0%
GTCCATTGCTCTCAATGAGA+AGG + chr2_4:56146615-56146634 Msa0336680:CDS 45.0%
TAGAGTGAATTGCAACCACC+TGG + chr2_4:56147588-56147607 Msa0336680:intron 45.0%
! TGGGCAGCAGCAACATTTTA+CGG + chr2_4:56147722-56147741 Msa0336680:intron 45.0%
! ATTGACTCAATCCAAGGGCT+GGG + chr2_4:56148061-56148080 Msa0336680:CDS 45.0%
TGGCAAAGAGGTCTTGAGAA+GGG - chr2_4:56148147-56148166 None:intergenic 45.0%
ATTGAAGGGATGTGGCAAAG+AGG - chr2_4:56148159-56148178 None:intergenic 45.0%
CCCTTCAATTTCGGTTACTG+AGG + chr2_4:56148170-56148189 Msa0336680:CDS 45.0%
GCCTCAGTAACCGAAATTGA+AGG - chr2_4:56148174-56148193 None:intergenic 45.0%
GGTTACTGAGGCATAATTGC+TGG + chr2_4:56148182-56148201 Msa0336680:CDS 45.0%
TTGTGGGGGACAAAACCAAA+AGG + chr2_4:56148571-56148590 Msa0336680:intron 45.0%
GTCCCAATGCAATTTCAACG+TGG + chr2_4:56148644-56148663 Msa0336680:intron 45.0%
ATGCAAGGGTGGAAACATTG+GGG - chr2_4:56149054-56149073 None:intergenic 45.0%
! TTGATGGCTAAATGCAAGGG+TGG - chr2_4:56149065-56149084 None:intergenic 45.0%
ACGAGCCCTTGAAAATTCCA+TGG - chr2_4:56149614-56149633 None:intergenic 45.0%
CTACTTCAGGACTAAGGTGT+GGG - chr2_4:56149651-56149670 None:intergenic 45.0%
!! TAGGTGCTTTGGAAGGGTTA+AGG - chr2_4:56149692-56149711 None:intergenic 45.0%
! CCATTCTTAGGTGCTTTGGA+AGG - chr2_4:56149699-56149718 None:intergenic 45.0%
! CCTTCCAAAGCACCTAAGAA+TGG + chr2_4:56149696-56149715 Msa0336680:CDS 45.0%
CTTGCCATTCTTAGGTGCTT+TGG - chr2_4:56149703-56149722 None:intergenic 45.0%
! CAAAGCACCTAAGAATGGCA+AGG + chr2_4:56149701-56149720 Msa0336680:CDS 45.0%
TCACATGGTCAAGAGACCAT+TGG - chr2_4:56150061-56150080 None:intergenic 45.0%
!! GATTAGGTGGTGGAAAGAGT+TGG + chr2_4:56150359-56150378 Msa0336680:intron 45.0%
GAGTGCTTCTTATGGACAGT+AGG + chr2_4:56150423-56150442 Msa0336680:CDS 45.0%
GAAGTTGCTCCATTGCATCA+AGG - chr2_4:56150692-56150711 None:intergenic 45.0%
TTGAGAGACCATCACCAATG+AGG - chr2_4:56151053-56151072 None:intergenic 45.0%
!! CATTGGTGATGGTCTCTCAA+AGG + chr2_4:56151053-56151072 Msa0336680:CDS 45.0%
CCATAGTCGAAGAATTTCCC+CGG - chr2_4:56151127-56151146 None:intergenic 45.0%
CGGGGAAATTCTTCGACTAT+GGG + chr2_4:56151125-56151144 Msa0336680:CDS 45.0%
CTTCGACTATGGGATGACTT+AGG + chr2_4:56151135-56151154 Msa0336680:CDS 45.0%
! GTCATGCTAACTTGTGCTCT+GGG + chr2_4:56151211-56151230 Msa0336680:intron 45.0%
! TCATGCTAACTTGTGCTCTG+GGG + chr2_4:56151212-56151231 Msa0336680:intron 45.0%
GTTCAGGAGGAACAAGAAAG+AGG + chr2_4:56151793-56151812 Msa0336680:intron 45.0%
GGATGCTACTAGGGAATTTG+TGG + chr2_4:56151893-56151912 Msa0336680:CDS 45.0%
!!! ATTTCATATATTTAATTTAA+CGG - chr2_4:56148410-56148429 None:intergenic 5.0%
!! GAAAATTAATTAATAAATAA+AGG + chr2_4:56151188-56151207 Msa0336680:intron 5.0%
!!! AATTTAAATATTTTTATTAG+TGG + chr2_4:56151391-56151410 Msa0336680:intron 5.0%
TGAATTGCAACCACCTGGCT+TGG + chr2_4:56147593-56147612 Msa0336680:intron 50.0%
! GATTGACTCAATCCAAGGGC+TGG + chr2_4:56148060-56148079 Msa0336680:CDS 50.0%
TCAATCCAAGGGCTGGGAAT+TGG + chr2_4:56148067-56148086 Msa0336680:CDS 50.0%
GCTGGGAATTGGTCACCATT+TGG + chr2_4:56148078-56148097 Msa0336680:CDS 50.0%
GTGGCAAAGAGGTCTTGAGA+AGG - chr2_4:56148148-56148167 None:intergenic 50.0%
CCTACTTCAGGACTAAGGTG+TGG - chr2_4:56149652-56149671 None:intergenic 50.0%
CCACACCTTAGTCCTGAAGT+AGG + chr2_4:56149649-56149668 Msa0336680:CDS 50.0%
ACCTTAGTCCTGAAGTAGGC+AGG + chr2_4:56149653-56149672 Msa0336680:CDS 50.0%
CCAATTTGCCTGCCTACTTC+AGG - chr2_4:56149664-56149683 None:intergenic 50.0%
CCTGAAGTAGGCAGGCAAAT+TGG + chr2_4:56149661-56149680 Msa0336680:CDS 50.0%
! GACCAACGGAGTGCTTCTTA+TGG + chr2_4:56150415-56150434 Msa0336680:CDS 50.0%
!! GTCCATAAGAAGCACTCCGT+TGG - chr2_4:56150420-56150439 None:intergenic 50.0%
CAGATGGGACCTTGATGCAA+TGG + chr2_4:56150680-56150699 Msa0336680:intron 50.0%
ATGCATGCCACCTTCCTCAT+TGG + chr2_4:56151036-56151055 Msa0336680:CDS 50.0%
ACCATCACCAATGAGGAAGG+TGG - chr2_4:56151046-56151065 None:intergenic 50.0%
GAGACCATCACCAATGAGGA+AGG - chr2_4:56151049-56151068 None:intergenic 50.0%
CCGGGGAAATTCTTCGACTA+TGG + chr2_4:56151124-56151143 Msa0336680:CDS 50.0%
! GGTCATGCTAACTTGTGCTC+TGG + chr2_4:56151210-56151229 Msa0336680:intron 50.0%
AATTGCTGGCCCACATGTCC+TGG + chr2_4:56148196-56148215 Msa0336680:CDS 55.0%
GCCTGCCTACTTCAGGACTA+AGG - chr2_4:56149657-56149676 None:intergenic 55.0%
GCCACCTTCCTCATTGGTGA+TGG + chr2_4:56151042-56151061 Msa0336680:CDS 55.0%
GGTGACCAATTCCCAGCCCT+TGG - chr2_4:56148075-56148094 None:intergenic 60.0%
CCGGAGCAAGTGAAGAGCCT+GGG - chr2_4:56151108-56151127 None:intergenic 60.0%
CCCAGGCTCTTCACTTGCTC+CGG + chr2_4:56151105-56151124 Msa0336680:CDS 60.0%
CCAGGCTCTTCACTTGCTCC+GGG + chr2_4:56151106-56151125 Msa0336680:CDS 60.0%
CAGGCTCTTCACTTGCTCCG+GGG + chr2_4:56151107-56151126 Msa0336680:CDS 60.0%
CCCGGAGCAAGTGAAGAGCC+TGG - chr2_4:56151109-56151128 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 56146432 56152252 56146432 ID=Msa0336680;Name=Msa0336680
chr2_4 mRNA 56146432 56152252 56146432 ID=Msa0336680-mRNA-1;Parent=Msa0336680;Name=Msa0336680-mRNA-1;_AED=0.04;_eAED=0.04;_QI=37|1|1|1|1|1|7|160|569
chr2_4 exon 56146432 56146636 56146432 ID=Msa0336680-mRNA-1:exon:17108;Parent=Msa0336680-mRNA-1
chr2_4 exon 56147953 56148217 56147953 ID=Msa0336680-mRNA-1:exon:17109;Parent=Msa0336680-mRNA-1
chr2_4 exon 56149482 56149854 56149482 ID=Msa0336680-mRNA-1:exon:17110;Parent=Msa0336680-mRNA-1
chr2_4 exon 56150365 56150583 56150365 ID=Msa0336680-mRNA-1:exon:17111;Parent=Msa0336680-mRNA-1
chr2_4 exon 56150683 56150821 56150683 ID=Msa0336680-mRNA-1:exon:17112;Parent=Msa0336680-mRNA-1
chr2_4 exon 56150916 56151167 56150916 ID=Msa0336680-mRNA-1:exon:17113;Parent=Msa0336680-mRNA-1
chr2_4 exon 56151799 56152252 56151799 ID=Msa0336680-mRNA-1:exon:17114;Parent=Msa0336680-mRNA-1
chr2_4 five_prime_UTR 56146432 56146468 56146432 ID=Msa0336680-mRNA-1:five_prime_utr;Parent=Msa0336680-mRNA-1
chr2_4 CDS 56146469 56146636 56146469 ID=Msa0336680-mRNA-1:cds;Parent=Msa0336680-mRNA-1
chr2_4 CDS 56147953 56148217 56147953 ID=Msa0336680-mRNA-1:cds;Parent=Msa0336680-mRNA-1
chr2_4 CDS 56149482 56149854 56149482 ID=Msa0336680-mRNA-1:cds;Parent=Msa0336680-mRNA-1
chr2_4 CDS 56150365 56150583 56150365 ID=Msa0336680-mRNA-1:cds;Parent=Msa0336680-mRNA-1
chr2_4 CDS 56150683 56150821 56150683 ID=Msa0336680-mRNA-1:cds;Parent=Msa0336680-mRNA-1
chr2_4 CDS 56150916 56151167 56150916 ID=Msa0336680-mRNA-1:cds;Parent=Msa0336680-mRNA-1
chr2_4 CDS 56151799 56152092 56151799 ID=Msa0336680-mRNA-1:cds;Parent=Msa0336680-mRNA-1
chr2_4 three_prime_UTR 56152093 56152252 56152093 ID=Msa0336680-mRNA-1:three_prime_utr;Parent=Msa0336680-mRNA-1
Gene Sequence

>Msa0336680

ATGACAATGGCTGTTTCTGAACTTAGAGTTTCATATCTAACAAGCACACTTGCTCGTTGTAATTTTGGGACTTCTACATATCAATCACGCATCTTATCGCTACAAAAATCAAATTCAAGGATTATGCATGGTAAATTATCGATCAAGTCCATTGCTCTCAATGAGAAGTTGTCACCTTTACAAACTGAAGATAAGACCAAAAGTTTGGGTCAAGTCAAAAGAAGAAGCCGAGAAGCATTGCTAAATTCAAATGATCCTATTGTAACATTGAAGATGATTGACTCAATCCAAGGGCTGGGAATTGGTCACCATTTGGAAGATGAGATCAATGTTCAACTTGGGAGGATATGTGATTGGGACCCTTCTCAAGACCTCTTTGCCACATCCCTTCAATTTCGGTTACTGAGGCATAATTGCTGGCCCACATGTCCTGATGTTTTCAGGAATTTTTTGGACAAGAGTGGCAATTTTAGGGAATCACTTACCAATGACATTTGGGGTATGTTGAGTTTATATGAGGCATCATATTTAGGGACAGAAGATGAAGAAATATTAAAAAAAGCCATGGAATTTTCAAGGGCTCGTCTAAGTGAATTAATCCCACACCTTAGTCCTGAAGTAGGCAGGCAAATTGGAAGAGCCTTAACCCTTCCAAAGCACCTAAGAATGGCAAGGTTAGAAGCTAGAAACTACATTGAAGAATATAGTCAAGAAAGTAACCAAATACCAACTCTTTTGGAATTGGCAAAGTTAGACTCTGAGATGATCCAGTCATTACACCAAAGAGAATTGGCAGAAATATGCAGGTGGTGGAAAGAGTTGGGACTTGTTGACAAACTTGGTTTTGCAAGAGATAGACCAACGGAGTGCTTCTTATGGACAGTAGGGATATTTCCAGAACCATGTCATTCAAATTGCCGCATTGAACTTACAAAAACCATATGTATTTTGCTAGTTATGGATGATATCTTTGACACTTATGGCACATTAGATGAACTTGTTCTTTTCACAAAGGCAATCAAAAGATGGGACCTTGATGCAATGGAGCAACTTCCTGAATACATGAAGATATGCTATATGGCATTATATAACACCACAAATGAAATTGCATATAGAATTCAGAAAGAACATGGACTAACCGTTGTTTCTCACCTGAAAAGAACATGGATTGACATATTTGAAGCATTTCTTGAAGAAGCTAAATGGTTCAATAATGGAAGTGTGCCAAATTTTAAGACATATTTGGATAATGGAGTGATTTCTGCAGGATCATGTATGGCATTGATGCATGCCACCTTCCTCATTGGTGATGGTCTCTCAAAGGAAACTATTTCCATGATGATGAAGTCATATCCCAGGCTCTTCACTTGCTCCGGGGAAATTCTTCGACTATGGGATGACTTAGGAACTTCAACGGAGGAACAAGAAAGAGGAGATAATGCTAGCAGTATACAATGCATGATGAGAGAGAATAATATTTCAGATGAAAATGAAGGAAGAATACAAATAAGGATGCTACTAGGGAATTTGTGGAGAGAACTCAATGGTCTTGCCATGACCAAAAACATACCTTCGTCTGTTATCAAAGCTTCTCTTAACATGGCTAGAACTGCTCAAGTTATTTATCAACATGGAGATGATCAAAGCACATTTACTGTTGATGACTATGTGCAAACCTTGATCTTCACATCATTACCTACTAAAAATTGA
Protein sequence

>Msa0336680

MTMAVSELRVSYLTSTLARCNFGTSTYQSRILSLQKSNSRIMHGKLSIKSIALNEKLSPLQTEDKTKSLGQVKRRSREALLNSNDPIVTLKMIDSIQGLGIGHHLEDEINVQLGRICDWDPSQDLFATSLQFRLLRHNCWPTCPDVFRNFLDKSGNFRESLTNDIWGMLSLYEASYLGTEDEEILKKAMEFSRARLSELIPHLSPEVGRQIGRALTLPKHLRMARLEARNYIEEYSQESNQIPTLLELAKLDSEMIQSLHQRELAEICRWWKELGLVDKLGFARDRPTECFLWTVGIFPEPCHSNCRIELTKTICILLVMDDIFDTYGTLDELVLFTKAIKRWDLDAMEQLPEYMKICYMALYNTTNEIAYRIQKEHGLTVVSHLKRTWIDIFEAFLEEAKWFNNGSVPNFKTYLDNGVISAGSCMALMHATFLIGDGLSKETISMMMKSYPRLFTCSGEILRLWDDLGTSTEEQERGDNASSIQCMMRENNISDENEGRIQIRMLLGNLWRELNGLAMTKNIPSSVIKASLNMARTAQVIYQHGDDQSTFTVDDYVQTLIFTSLPTKN*