AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0299340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299340 MtrunA17_Chr2g0323131 92.769 567 41 0 3 569 1 567 0.0 1104
Msa0299340 MtrunA17_Chr2g0323151 85.208 480 70 1 87 566 1 479 0.0 876
Msa0299340 MtrunA17_Chr4g0023911 40.081 494 276 8 64 546 74 558 1.67e-117 360
Msa0299340 MtrunA17_Chr4g0042111 36.832 505 298 7 57 546 41 539 5.70e-110 340
Msa0299340 MtrunA17_Chr2g0310331 35.943 562 348 6 15 566 26 585 6.71e-109 338
Msa0299340 MtrunA17_Chr4g0048911 33.631 559 343 11 21 561 6 554 1.55e-105 328
Msa0299340 MtrunA17_Chr4g0048921 35.882 510 313 7 62 562 62 566 5.07e-102 319
Msa0299340 MtrunA17_Chr6g0451711 35.070 499 302 8 83 566 65 556 3.02e-99 312
Msa0299340 MtrunA17_Chr4g0024691 34.328 536 325 11 45 566 38 560 1.78e-98 310
Msa0299340 MtrunA17_Chr2g0310401 39.675 431 250 5 142 566 5 431 2.00e-97 303
Msa0299340 MtrunA17_Chr6g0465441 32.745 510 328 8 69 566 46 552 1.43e-96 305
Msa0299340 MtrunA17_Chr8g0357411 34.659 528 332 7 50 566 63 588 3.98e-96 305
Msa0299340 MtrunA17_Chr3g0099031 33.533 501 319 6 74 561 13 512 6.13e-96 302
Msa0299340 MtrunA17_Chr7g0236961 34.664 476 293 6 86 549 9 478 7.52e-96 301
Msa0299340 MtrunA17_Chr7g0237011 34.810 474 291 6 86 547 9 476 1.47e-95 300
Msa0299340 MtrunA17_Chr2g0280581 33.202 506 325 4 69 561 12 517 1.47e-95 301
Msa0299340 MtrunA17_Chr7g0236991 34.146 492 305 7 69 547 46 531 1.96e-95 301
Msa0299340 MtrunA17_Chr7g0237001 34.146 492 305 7 69 547 46 531 3.17e-95 301
Msa0299340 MtrunA17_Chr7g0237041 34.146 492 305 7 69 547 46 531 3.17e-95 301
Msa0299340 MtrunA17_Chr7g0237081 34.146 492 305 7 69 547 46 531 3.17e-95 301
Msa0299340 MtrunA17_Chr7g0237051 34.146 492 305 7 69 547 46 531 3.17e-95 301
Msa0299340 MtrunA17_Chr3g0109001 33.730 504 315 5 74 561 13 513 5.71e-94 297
Msa0299340 MtrunA17_Chr3g0108941 34.051 511 316 7 72 565 11 517 1.08e-93 296
Msa0299340 MtrunA17_Chr5g0425371 33.922 510 316 8 72 566 49 552 2.06e-93 296
Msa0299340 MtrunA17_Chr3g0108981 33.742 489 305 5 89 561 3 488 8.90e-93 293
Msa0299340 MtrunA17_Chr3g0108961 33.742 489 305 5 89 561 3 488 8.90e-93 293
Msa0299340 MtrunA17_Chr6g0477111 34.774 486 297 7 92 561 6 487 5.60e-92 291
Msa0299340 MtrunA17_Chr3g0108991 33.988 509 318 9 72 565 11 516 6.16e-92 291
Msa0299340 MtrunA17_Chr3g0108951 33.988 509 318 9 72 565 11 516 6.16e-92 291
Msa0299340 MtrunA17_Chr3g0109021 33.598 503 316 9 74 561 13 512 7.55e-92 291
Msa0299340 MtrunA17_Chr3g0105071 34.792 480 290 7 83 547 64 535 7.72e-92 292
Msa0299340 MtrunA17_Chr3g0108971 34.756 492 303 9 89 565 3 491 2.46e-91 289
Msa0299340 MtrunA17_Chr3g0082691 33.267 502 310 8 82 566 59 552 6.82e-91 290
Msa0299340 MtrunA17_Chr3g0109031 31.881 505 330 5 74 565 13 516 2.25e-90 288
Msa0299340 MtrunA17_Chr2g0319051 34.325 504 312 9 73 561 9 508 2.51e-90 287
Msa0299340 MtrunA17_Chr3g0105691 34.146 492 310 7 86 564 58 548 5.15e-90 288
Msa0299340 MtrunA17_Chr6g0465851 32.422 512 327 7 69 566 11 517 2.02e-89 285
Msa0299340 MtrunA17_Chr6g0465911 32.422 512 327 7 69 566 46 552 3.77e-89 285
Msa0299340 MtrunA17_Chr6g0466051 32.422 512 327 7 69 566 46 552 4.19e-89 285
Msa0299340 MtrunA17_Chr5g0431651 33.465 505 305 9 82 566 58 551 3.03e-88 283
Msa0299340 MtrunA17_Chr3g0109011 33.129 489 307 7 89 561 3 487 3.22e-88 281
Msa0299340 MtrunA17_Chr2g0319041 32.375 522 329 11 59 561 35 551 1.14e-87 282
Msa0299340 MtrunA17_Chr5g0431571 34.686 493 296 9 72 547 44 527 9.93e-86 276
Msa0299340 MtrunA17_Chr8g0336181 33.529 510 307 11 62 550 39 537 1.15e-84 274
Msa0299340 MtrunA17_Chr6g0477121 35.422 415 256 6 143 549 8 418 3.53e-81 260
Msa0299340 MtrunA17_Chr7g0236951 40.193 311 173 2 86 386 9 316 3.60e-76 244
Msa0299340 MtrunA17_Chr7g0237031 40.193 311 173 2 86 386 9 316 5.34e-76 243
Msa0299340 MtrunA17_Chr7g0236971 34.115 384 248 4 168 549 1 381 1.82e-74 242
Msa0299340 MtrunA17_Chr6g0466111 39.091 330 187 3 69 387 11 337 1.29e-72 235
Msa0299340 MtrunA17_Chr6g0465981 39.091 330 187 3 69 387 46 372 2.44e-72 236
Msa0299340 MtrunA17_Chr6g0465871 39.091 330 187 3 69 387 46 372 2.44e-72 236
Msa0299340 MtrunA17_Chr6g0466081 39.091 330 187 3 69 387 46 372 2.44e-72 236
Msa0299340 MtrunA17_Chr6g0465931 40.260 308 171 2 90 387 33 337 1.55e-71 233
Msa0299340 MtrunA17_Chr4g0008621 35.112 356 219 4 84 428 70 424 1.48e-64 217
Msa0299340 MtrunA17_Chr7g0217361 39.427 279 165 4 270 546 11 287 1.02e-60 203
Msa0299340 MtrunA17_Chr5g0445511 40.336 238 142 0 164 401 2 239 9.93e-54 182
Msa0299340 MtrunA17_Chr2g0319071 27.439 492 295 12 90 567 46 489 6.61e-48 174
Msa0299340 MtrunA17_Chr8g0357231 32.308 260 171 4 310 566 1 258 1.07e-46 164
Msa0299340 MtrunA17_Chr2g0310121 25.911 494 330 12 85 546 271 760 1.00e-39 154
Msa0299340 MtrunA17_Chr7g0237071 38.614 202 108 3 86 274 9 207 8.37e-37 136
Msa0299340 MtrunA17_Chr3g0101681 25.050 503 338 13 90 560 277 772 1.12e-36 145
Msa0299340 MtrunA17_Chr2g0319081 30.872 298 184 9 270 561 140 421 4.00e-36 140
Msa0299340 MtrunA17_Chr6g0466031 25.769 260 188 4 310 566 2 259 2.14e-28 114
Msa0299340 MtrunA17_Chr8g0357221 32.692 260 161 5 18 264 27 285 2.79e-27 111
Msa0299340 MtrunA17_Chr7g0237061 27.801 241 148 6 310 549 2 217 2.88e-27 110
Msa0299340 MtrunA17_Chr2g0281651 26.415 318 220 7 245 555 43 353 3.27e-27 114
Msa0299340 MtrunA17_Chr7g0233861 40.650 123 64 1 220 342 45 158 1.61e-22 95.1
Msa0299340 MtrunA17_Chr6g0466021 37.419 155 83 3 72 215 49 200 1.64e-22 95.9
Msa0299340 MtrunA17_Chr7g0217351 31.628 215 130 6 66 270 66 273 2.12e-20 92.0
Msa0299340 MtrunA17_Chr7g0259881 29.167 288 160 11 92 340 232 514 3.84e-20 94.7
Msa0299340 MtrunA17_Chr7g0218001 28.352 261 150 7 92 317 349 607 8.66e-17 84.3
Msa0299340 MtrunA17_Chr2g0281721 35.000 100 63 2 245 343 29 127 1.32e-12 65.5
Msa0299340 MtrunA17_Chr7g0218041 30.660 212 121 6 130 317 1 210 1.32e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299340 AT4G16730.1 40.661 514 282 7 73 566 28 538 1.13e-131 395
Msa0299340 AT4G16730.2 41.212 495 268 7 92 566 1 492 1.19e-130 391
Msa0299340 AT4G16740.1 37.023 524 299 8 63 566 51 563 1.33e-110 342
Msa0299340 AT1G61680.1 36.550 487 299 6 82 561 86 569 2.27e-105 328
Msa0299340 AT2G24210.1 35.758 495 296 9 90 566 99 589 3.58e-104 326
Msa0299340 AT3G25820.1 35.115 524 304 11 65 566 84 593 7.97e-103 323
Msa0299340 AT3G25830.1 35.115 524 304 11 65 566 84 593 7.97e-103 323
Msa0299340 AT3G25810.1 34.800 500 299 10 90 566 101 596 1.13e-99 315
Msa0299340 AT1G61680.2 35.729 487 296 7 82 561 86 562 2.99e-99 312
Msa0299340 AT5G23960.2 31.154 520 345 7 58 566 27 544 1.53e-86 279
Msa0299340 AT5G23960.1 31.226 522 344 8 58 566 27 546 2.19e-85 276
Msa0299340 AT1G70080.1 32.449 490 303 11 92 560 122 604 8.32e-79 260
Msa0299340 AT4G16740.2 38.192 343 189 5 63 388 51 387 4.57e-72 236
Msa0299340 AT3G25820.2 36.415 357 199 8 65 406 84 427 1.23e-69 231
Msa0299340 AT3G14490.1 30.572 507 332 10 72 565 95 594 3.09e-67 229
Msa0299340 AT1G33750.1 29.198 524 352 9 54 566 87 602 1.35e-65 225
Msa0299340 AT3G29410.2 30.128 468 311 7 90 546 13 475 1.54e-65 222
Msa0299340 AT3G29410.1 30.128 468 311 7 90 546 118 580 1.19e-64 222
Msa0299340 AT1G31950.3 28.330 533 359 9 50 566 81 606 9.86e-63 217
Msa0299340 AT1G31950.1 29.401 534 351 11 50 566 81 605 1.07e-62 217
Msa0299340 AT3G14540.1 30.316 475 310 8 90 549 113 581 3.59e-62 216
Msa0299340 AT4G15870.1 28.015 539 355 13 20 547 60 576 1.42e-60 211
Msa0299340 AT3G14520.1 28.481 474 320 7 90 549 116 584 2.08e-58 205
Msa0299340 AT5G44630.1 30.398 477 303 12 87 546 70 534 2.30e-56 199
Msa0299340 AT4G16740.4 36.478 318 179 5 63 363 51 362 3.08e-55 191
Msa0299340 AT4G16740.3 36.478 318 179 5 63 363 51 362 3.08e-55 191
Msa0299340 AT3G32030.1 26.419 511 357 7 72 567 98 604 3.13e-55 197
Msa0299340 AT4G13300.1 28.571 511 348 9 66 561 40 548 3.70e-55 196
Msa0299340 AT1G31950.2 29.724 434 285 7 50 470 81 507 3.01e-53 190
Msa0299340 AT2G23230.2 29.848 526 324 14 66 561 84 594 4.63e-53 191
Msa0299340 AT3G29110.1 28.968 504 321 11 67 546 84 574 9.34e-53 190
Msa0299340 AT4G20230.1 27.983 461 320 7 92 542 119 577 1.26e-52 189
Msa0299340 AT2G23230.1 29.942 521 325 14 66 561 84 589 1.27e-52 189
Msa0299340 AT4G20230.2 27.983 461 320 7 92 542 131 589 1.29e-52 190
Msa0299340 AT4G13280.2 28.924 446 308 6 123 561 105 548 2.45e-52 188
Msa0299340 AT3G25820.4 36.860 293 157 8 65 342 84 363 7.36e-52 182
Msa0299340 AT3G25820.3 36.860 293 157 8 65 342 84 363 7.36e-52 182
Msa0299340 AT3G14540.2 30.886 395 253 7 90 470 113 501 8.67e-52 185
Msa0299340 AT3G29190.1 29.710 414 281 6 92 496 116 528 1.50e-51 186
Msa0299340 AT4G20210.1 28.081 495 324 11 92 565 115 598 1.74e-50 184
Msa0299340 AT5G48110.1 29.375 320 221 4 228 546 2 317 1.90e-50 177
Msa0299340 AT1G66020.1 29.247 465 309 9 92 542 113 571 4.20e-50 182
Msa0299340 AT1G48800.1 29.645 479 311 13 90 552 118 586 8.33e-50 182
Msa0299340 AT4G20200.1 28.664 464 313 6 92 542 119 577 8.88e-50 182
Msa0299340 AT1G66020.2 36.071 280 162 6 92 358 113 388 1.51e-43 161
Msa0299340 AT1G48820.1 26.994 489 311 12 67 547 92 542 3.67e-43 162
Msa0299340 AT4G13280.1 27.578 446 284 8 123 561 105 518 6.89e-43 161
Msa0299340 AT1G79460.1 25.536 513 339 13 85 562 270 774 4.73e-34 138
Msa0299340 AT1G61120.1 26.714 423 272 7 84 478 280 692 5.51e-32 132
Msa0299340 AT4G02780.1 30.268 261 145 8 92 317 334 592 2.41e-19 92.8

Find 121 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTTGCTCGTTGTAATTT+TGG 0.081136 2_3:+54171147 Msa0299340:CDS
TCCAAATATGTCTTAAAATT+TGG 0.090930 2_3:-54175593 None:intergenic
TTATATGAGGCATCATATTT+AGG 0.175329 2_3:+54174177 Msa0299340:CDS
ATTCCAAAAGAGTTGGTATT+TGG 0.197928 2_3:-54174386 None:intergenic
CCGGGGAAATTCTTCGACTA+TGG 0.229539 2_3:+54175741 Msa0299340:CDS
TTTAAGACATATTTGGATAA+TGG 0.251513 2_3:+54175599 Msa0299340:CDS
TAACCAAATACCAACTCTTT+TGG 0.266679 2_3:+54174383 Msa0299340:CDS
TATATGAGGCATCATATTTA+GGG 0.283045 2_3:+54174178 Msa0299340:CDS
ATATTTCTGCCAATTCTCTT+TGG 0.285413 2_3:-54174446 None:intergenic
TGAAGATAAGACCAAAAGTT+TGG 0.285905 2_3:+54172588 Msa0299340:CDS
GCCATTGTCATTGTATTTGC+AGG 0.288475 2_3:-54171090 None:intergenic
ATCACTTACCAATGACATTT+GGG 0.292181 2_3:+54174143 Msa0299340:CDS
GATGATATCTTTGACACTTA+TGG 0.293561 2_3:+54175136 Msa0299340:CDS
TTTGACTTGACCCAAACTTT+TGG 0.296123 2_3:-54172599 None:intergenic
CCAATTTGCCTGCCTACTTC+AGG 0.296197 2_3:-54174279 None:intergenic
TAGCATATCTTCATGTATTC+AGG 0.305968 2_3:-54175328 None:intergenic
CATTTCTTGAAGAAGCTAAA+TGG 0.310663 2_3:+54175552 Msa0299340:CDS
CTTCTAACCTTGCCATTCTT+AGG 0.311069 2_3:-54174326 None:intergenic
CAATTTGAATGACATGGTTC+TGG 0.331181 2_3:-54175070 None:intergenic
TTGCCACATCCCTTCAATTT+CGG 0.335451 2_3:+54172779 Msa0299340:CDS
CTTGCCATTCTTAGGTGCTT+TGG 0.346897 2_3:-54174318 None:intergenic
AATCACTTACCAATGACATT+TGG 0.351614 2_3:+54174142 Msa0299340:CDS
CCTGAAGTAGGCAGGCAAAT+TGG 0.353627 2_3:+54174279 Msa0299340:CDS
ATGAAGGAAGAATACAAATA+AGG 0.356949 2_3:+54176497 Msa0299340:CDS
TCAATCCAAGGGCTGGGAAT+TGG 0.367737 2_3:+54172685 Msa0299340:CDS
CCCAGGCTCTTCACTTGCTC+CGG 0.372004 2_3:+54175722 Msa0299340:CDS
ATACATGAAGATATGCTATA+TGG 0.378469 2_3:+54175333 Msa0299340:CDS
TCTATATGCAATTTCATTTG+TGG 0.378965 2_3:-54175368 None:intergenic
GACCAACGGAGTGCTTCTTA+TGG 0.384882 2_3:+54175032 Msa0299340:CDS
ATTAGGTGGTGGAAAGAGTT+GGG 0.386750 2_3:+54174977 Msa0299340:intron
GAAGCTAAATGGTTCAATAA+TGG 0.388219 2_3:+54175563 Msa0299340:CDS
GCTGGGAATTGGTCACCATT+TGG 0.393146 2_3:+54172696 Msa0299340:CDS
GTCATTACACCAAAGAGAAT+TGG 0.404125 2_3:+54174437 Msa0299340:CDS
TTGAATTTAGCAATGCTTCT+CGG 0.408026 2_3:-54172630 None:intergenic
TTTCGGTTACTGAGGCATAA+TGG 0.414627 2_3:+54172796 Msa0299340:CDS
AACTTGGGAGGATATGTGAT+TGG 0.414855 2_3:+54172737 Msa0299340:CDS
ATACAAATAAGGATGCTACT+AGG 0.418565 2_3:+54176508 Msa0299340:CDS
CCATTCTTAGGTGCTTTGGA+AGG 0.418608 2_3:-54174314 None:intergenic
GGGTATGTTGAGTTTATATG+AGG 0.434234 2_3:+54174164 Msa0299340:CDS
ATCATCTTCAATGTTACAAT+AGG 0.441194 2_3:-54172658 None:intergenic
GGATGCTACTAGGGAATTTG+TGG 0.444878 2_3:+54176518 Msa0299340:CDS
GAAGATAAGACCAAAAGTTT+GGG 0.446624 2_3:+54172589 Msa0299340:CDS
ATTCTCTTTGGTGTAATGAC+TGG 0.447027 2_3:-54174434 None:intergenic
TGTGTTATCACAAATATAGA+AGG 0.448145 2_3:+54171056 None:intergenic
ATGCATGCCACCTTCCTCAT+TGG 0.449603 2_3:+54175653 Msa0299340:CDS
TAGGTGCTTTGGAAGGGTTA+AGG 0.456059 2_3:-54174307 None:intergenic
GCCTGCCTACTTCAGGACTA+AGG 0.459721 2_3:-54174272 None:intergenic
CAACATACCCCAAATGTCAT+TGG 0.463701 2_3:-54174151 None:intergenic
CATAACTAGCAAAATACATA+TGG 0.467636 2_3:-54175113 None:intergenic
CCAGGCTCTTCACTTGCTCC+GGG 0.468250 2_3:+54175723 Msa0299340:CDS
GGTGACCAATTCCCAGCCCT+TGG 0.469761 2_3:-54172690 None:intergenic
GATAATGGAGTGATTTCTGC+AGG 0.473286 2_3:+54175614 Msa0299340:CDS
TTGGGACTTGTTGACAAACT+TGG 0.473750 2_3:+54174995 Msa0299340:CDS
GATTGACTCAATCCAAGGGC+TGG 0.474173 2_3:+54172678 Msa0299340:CDS
GTCTTATCTTCAGTTTGTAA+AGG 0.481471 2_3:-54172577 None:intergenic
GATTAGGTGGTGGAAAGAGT+TGG 0.483866 2_3:+54174976 Msa0299340:intron
TACAAATAAGGATGCTACTA+GGG 0.488175 2_3:+54176509 Msa0299340:CDS
CTTCGACTATGGGATGACTT+AGG 0.488396 2_3:+54175752 Msa0299340:CDS
CGGGGAAATTCTTCGACTAT+GGG 0.489879 2_3:+54175742 Msa0299340:CDS
AATGGCTGGCCCACATGTCC+TGG 0.494407 2_3:+54172814 Msa0299340:CDS
CATTGATCTCATCTTCCAAA+TGG 0.495809 2_3:-54172711 None:intergenic
ACTTGGGAGGATATGTGATT+GGG 0.499523 2_3:+54172738 Msa0299340:CDS
GCCACCTTCCTCATTGGTGA+TGG 0.504456 2_3:+54175659 Msa0299340:CDS
CATTCTTAGGTGCTTTGGAA+GGG 0.508082 2_3:-54174313 None:intergenic
TTTGCCAATTCCAAAAGAGT+TGG 0.511202 2_3:-54174393 None:intergenic
GCCTCAGTAACCGAAATTGA+AGG 0.515765 2_3:-54172789 None:intergenic
AATATTTCAGATGAAAATGA+AGG 0.518222 2_3:+54176481 Msa0299340:CDS
AGATACAGATTCTATGCCTA+TGG 0.518901 2_3:+54176725 Msa0299340:three_prime_UTR
ATTGACTCAATCCAAGGGCT+GGG 0.520399 2_3:+54172679 Msa0299340:CDS
GATTTCTGCAGGATCATGTA+TGG 0.522815 2_3:+54175625 Msa0299340:CDS
GAGTGCTTCTTATGGACAGT+AGG 0.523662 2_3:+54175040 Msa0299340:CDS
CCTTCCAAAGCACCTAAGAA+TGG 0.525082 2_3:+54174314 Msa0299340:CDS
GGGATATGACTTCATCATCA+TGG 0.527240 2_3:-54175702 None:intergenic
ACCTTAGTCCTGAAGTAGGC+AGG 0.530056 2_3:+54174271 Msa0299340:CDS
GGTTACTGAGGCATAATGGC+TGG 0.530808 2_3:+54172800 Msa0299340:CDS
GAGAATTGGCAGAAATATGC+AGG 0.535303 2_3:+54174451 Msa0299340:CDS
CAGATGGGACCTTGATGCAA+TGG 0.538910 2_3:+54175297 Msa0299340:intron
CCCTTCAATTTCGGTTACTG+AGG 0.554070 2_3:+54172788 Msa0299340:CDS
ACGAGCCCTTGAAAATTCCA+TGG 0.555738 2_3:-54174229 None:intergenic
GTTCAGGAGGAACAAGAAAG+AGG 0.555777 2_3:+54176418 Msa0299340:intron
CCCGGAGCAAGTGAAGAGCC+TGG 0.558035 2_3:-54175723 None:intergenic
TAACCGAAATTGAAGGGATG+TGG 0.558085 2_3:-54172782 None:intergenic
AGGTAATGATGTGAAGATCA+AGG 0.564747 2_3:-54176683 None:intergenic
GATGAGATCAATGTTCAACT+TGG 0.572324 2_3:+54172721 Msa0299340:CDS
ATGCGGCAATTTGAATGACA+TGG 0.572463 2_3:-54175076 None:intergenic
AAGATGATTGACTCAATCCA+AGG 0.578634 2_3:+54172673 Msa0299340:CDS
GTCCATTGCTCTCAATGAGA+AGG 0.579260 2_3:+54171248 Msa0299340:CDS
CCACACCTTAGTCCTGAAGT+AGG 0.581171 2_3:+54174267 Msa0299340:CDS
CCTCAGTAACCGAAATTGAA+GGG 0.583119 2_3:-54172788 None:intergenic
CCGGAGCAAGTGAAGAGCCT+GGG 0.583946 2_3:-54175722 None:intergenic
TGATGATGAAGTCATATCCC+AGG 0.589646 2_3:+54175705 Msa0299340:CDS
CTACTTCAGGACTAAGGTGT+GGG 0.590174 2_3:-54174266 None:intergenic
TACCTTCTCATTGAGAGCAA+TGG 0.593656 2_3:-54171250 None:intergenic
TGCAATATACCAGGACATGT+GGG 0.593982 2_3:-54172823 None:intergenic
CATTGGTGATGGTCTCTCAA+AGG 0.594573 2_3:+54175670 Msa0299340:CDS
ATGAGATCAATGTTCAACTT+GGG 0.595916 2_3:+54172722 Msa0299340:CDS
GAAGTTGCTCCATTGCATCA+AGG 0.597358 2_3:-54175306 None:intergenic
TATCAAAGCTTCTCTTAACA+TGG 0.598838 2_3:+54176588 Msa0299340:CDS
ATGCAATATACCAGGACATG+TGG 0.603297 2_3:-54172824 None:intergenic
CCATAGTCGAAGAATTTCCC+CGG 0.608178 2_3:-54175741 None:intergenic
GTCCATAAGAAGCACTCCGT+TGG 0.609668 2_3:-54175034 None:intergenic
TCAAATTCAAGGATTATGCA+TGG 0.614482 2_3:+54171210 Msa0299340:CDS
AGTGCTTCTTATGGACAGTA+GGG 0.615566 2_3:+54175041 Msa0299340:CDS
AGATCAATGTTCAACTTGGG+AGG 0.619212 2_3:+54172725 Msa0299340:CDS
GTGGCAAAGAGGTCTTGAGA+AGG 0.619717 2_3:-54172763 None:intergenic
GCTCAAGTTATTTATCAACA+TGG 0.621098 2_3:+54176619 Msa0299340:CDS
GAGACCATCACCAATGAGGA+AGG 0.621909 2_3:-54175663 None:intergenic
CCTACTTCAGGACTAAGGTG+TGG 0.623254 2_3:-54174267 None:intergenic
CAAAGCACCTAAGAATGGCA+AGG 0.625330 2_3:+54174319 Msa0299340:CDS
TGGCAAAGAGGTCTTGAGAA+GGG 0.625651 2_3:-54172762 None:intergenic
GCCTGCAAATACAATGACAA+TGG 0.627016 2_3:+54171089 Msa0299340:exon
TATAGAATTCAGAAAGAACA+TGG 0.628836 2_3:+54175385 Msa0299340:CDS
GGATGACTTAGGAACTTCAA+CGG 0.635407 2_3:+54175763 Msa0299340:CDS
TTGAGAGACCATCACCAATG+AGG 0.655213 2_3:-54175667 None:intergenic
AGATGATTGACTCAATCCAA+GGG 0.655644 2_3:+54172674 Msa0299340:CDS
ATTGAAGGGATGTGGCAAAG+AGG 0.660331 2_3:-54172774 None:intergenic
TCACTTACCAATGACATTTG+GGG 0.667503 2_3:+54174144 Msa0299340:CDS
ACCATCACCAATGAGGAAGG+TGG 0.672270 2_3:-54175660 None:intergenic
CAGGCTCTTCACTTGCTCCG+GGG 0.678340 2_3:+54175724 Msa0299340:CDS
AATTTGTGGAGAGAACTCAA+TGG 0.695889 2_3:+54176532 Msa0299340:CDS
GAAGCTTTGATAACAGACGA+AGG 0.721487 2_3:-54176577 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTAATAAATAAA+GGG + chr2_3:54175806-54175825 Msa0299340:intron 0.0%
!! AAATTAGTTACTATAATTTA+GGG - chr2_3:54172116-54172135 None:intergenic 10.0%
!! AAAATTAGTTACTATAATTT+AGG - chr2_3:54172117-54172136 None:intergenic 10.0%
!!! AATCAGTTTTTAAAATAATA+TGG + chr2_3:54172450-54172469 Msa0299340:intron 10.0%
!! ACTCAATAAAAAAATAAATT+TGG - chr2_3:54172984-54173003 None:intergenic 10.0%
!! TTAAATATACATTATATAAG+AGG + chr2_3:54173147-54173166 Msa0299340:intron 10.0%
!!! TTTAATTAAATATTTTGTTG+TGG - chr2_3:54174498-54174517 None:intergenic 10.0%
!! ACAACAAAATATTTAATTAA+AGG + chr2_3:54174497-54174516 Msa0299340:intron 10.0%
!! AAAATTTCATCTTTAAATAT+CGG - chr2_3:54174780-54174799 None:intergenic 10.0%
!!! TTATTAATTAATTTTCAGTA+GGG - chr2_3:54175801-54175820 None:intergenic 10.0%
!!! TTTATTAATTAATTTTCAGT+AGG - chr2_3:54175802-54175821 None:intergenic 10.0%
!!! CAAATTTAAATTTTTATTAG+TGG + chr2_3:54176211-54176230 Msa0299340:intron 10.0%
!!! ATTGATGATTATATAAGATA+GGG - chr2_3:54171294-54171313 None:intergenic 15.0%
!!! TATTGATGATTATATAAGAT+AGG - chr2_3:54171295-54171314 None:intergenic 15.0%
!! TAAATTTAAATTCATTGTTG+TGG + chr2_3:54171529-54171548 Msa0299340:intron 15.0%
!!! AATTATAGTAACTAATTTTG+CGG + chr2_3:54172118-54172137 Msa0299340:intron 15.0%
!! AAATAAAAAGATGTGATTTA+CGG - chr2_3:54172408-54172427 None:intergenic 15.0%
!!! TCTTTTTATTTAGAAGTAAA+TGG + chr2_3:54172417-54172436 Msa0299340:intron 15.0%
!!! TCTAAGACAATATTTTATAA+GGG - chr2_3:54172498-54172517 None:intergenic 15.0%
!!! TTCTAAGACAATATTTTATA+AGG - chr2_3:54172499-54172518 None:intergenic 15.0%
!! AGAAATAAAAAGAATGAATT+TGG - chr2_3:54173094-54173113 None:intergenic 15.0%
!!! TTTTTATTTCTAACTTTAGT+GGG + chr2_3:54173103-54173122 Msa0299340:intron 15.0%
!!! CATTATATTTTTTTTTGACA+AGG + chr2_3:54173797-54173816 Msa0299340:intron 15.0%
!! ACAAAATATTTAATTAAAGG+TGG + chr2_3:54174500-54174519 Msa0299340:intron 15.0%
!!! ATTCAATATTGATTTAGATT+TGG - chr2_3:54174723-54174742 None:intergenic 15.0%
!!! CAAATTTAAATTTTTATCAG+TGG + chr2_3:54175954-54175973 Msa0299340:intron 15.0%
!!! AATATTTTTATTAGTGGTAA+AGG + chr2_3:54176021-54176040 Msa0299340:intron 15.0%
!!! TTTATTACAATTTGTTTCAT+AGG - chr2_3:54176352-54176371 None:intergenic 15.0%
!!! TTTTTCAAATAAAATACCAT+AGG - chr2_3:54176744-54176763 None:intergenic 15.0%
!!! TGGTATTTTATTTGAAAAAT+AGG + chr2_3:54176745-54176764 Msa0299340:three_prime_UTR 15.0%
!!! GGTATTTTATTTGAAAAATA+GGG + chr2_3:54176746-54176765 Msa0299340:three_prime_UTR 15.0%
!!! ATCATTTTTTTTTTTGTTGA+AGG + chr2_3:54176845-54176864 Msa0299340:three_prime_UTR 15.0%
!!! TAATCATCAATATGAAGTTT+TGG + chr2_3:54171303-54171322 Msa0299340:intron 20.0%
!!! AATCATCAATATGAAGTTTT+GGG + chr2_3:54171304-54171323 Msa0299340:intron 20.0%
!!! ACTTGTCAAAATCAATTTTA+AGG - chr2_3:54171413-54171432 None:intergenic 20.0%
!!! ATATCAAACTGATTTATGTT+TGG - chr2_3:54171624-54171643 None:intergenic 20.0%
!! AAGTTGAATATAAAAGTTCT+AGG + chr2_3:54171675-54171694 Msa0299340:intron 20.0%
!! AGCATCTTCTTAAAAAATAA+AGG + chr2_3:54171737-54171756 Msa0299340:intron 20.0%
!! GCATCTTCTTAAAAAATAAA+GGG + chr2_3:54171738-54171757 Msa0299340:intron 20.0%
!! TAAATCTTATGTAGAAATGA+AGG + chr2_3:54171783-54171802 Msa0299340:intron 20.0%
!!! TGTTTGTTTTAAGATGTTAT+TGG + chr2_3:54172320-54172339 Msa0299340:intron 20.0%
!!! GTTTGTTTTAAGATGTTATT+GGG + chr2_3:54172321-54172340 Msa0299340:intron 20.0%
!!! CTTTTTATTTCTAACTTTAG+TGG + chr2_3:54173102-54173121 Msa0299340:intron 20.0%
!! TTAAGAAAATGTTTCTTCTA+TGG - chr2_3:54173458-54173477 None:intergenic 20.0%
!!! TTTGTTGTCAAATAGTTTAA+TGG + chr2_3:54173499-54173518 Msa0299340:intron 20.0%
!! TGTAAGTATAATTCACTTAA+TGG - chr2_3:54173607-54173626 None:intergenic 20.0%
!! AATGTATTACTACTATATTC+AGG + chr2_3:54173962-54173981 Msa0299340:intron 20.0%
!!! TTTATAAGTATTGTATAAGC+AGG + chr2_3:54173985-54174004 Msa0299340:intron 20.0%
!! AGAAATATTAAAAAAAGCCA+TGG + chr2_3:54174212-54174231 Msa0299340:CDS 20.0%
!!! CTAGTTTATTTGATTTGATT+AGG + chr2_3:54174960-54174979 Msa0299340:intron 20.0%
!!! TAAACTTTATGTTTTACAGA+TGG + chr2_3:54175281-54175300 Msa0299340:intron 20.0%
!!! AAACTTTATGTTTTACAGAT+GGG + chr2_3:54175282-54175301 Msa0299340:intron 20.0%
!! TCCAAATATGTCTTAAAATT+TGG - chr2_3:54175596-54175615 None:intergenic 20.0%
!!! TTTAAGACATATTTGGATAA+TGG + chr2_3:54175599-54175618 Msa0299340:CDS 20.0%
!! TATAAATTTGTATCTACTCA+AGG - chr2_3:54176262-54176281 None:intergenic 20.0%
!!! TCTTACTAATTTTTATGTTC+AGG + chr2_3:54176402-54176421 Msa0299340:intron 20.0%
!! AATATTTCAGATGAAAATGA+AGG + chr2_3:54176481-54176500 Msa0299340:CDS 20.0%
!!! TAAATGACATATTTTTGTCA+TGG + chr2_3:54176822-54176841 Msa0299340:three_prime_UTR 20.0%
!!! AGTGTGTTTGTTTTTACTAT+AGG + chr2_3:54171367-54171386 Msa0299340:intron 25.0%
!!! GTGTGTTTGTTTTTACTATA+GGG + chr2_3:54171368-54171387 Msa0299340:intron 25.0%
!!! GTCAAAATCAATTTTAAGGT+TGG - chr2_3:54171409-54171428 None:intergenic 25.0%
!!! AATTGATTTTGACAAGTTTG+TGG + chr2_3:54171416-54171435 Msa0299340:intron 25.0%
! AATTGCAAGTGATTTATGTT+TGG - chr2_3:54171571-54171590 None:intergenic 25.0%
! TTGCAATTCAATCAATTTCA+TGG + chr2_3:54171583-54171602 Msa0299340:intron 25.0%
! AATTCAATCAATTTCATGGT+TGG + chr2_3:54171587-54171606 Msa0299340:intron 25.0%
! CAAATAACACACTGTATAAT+TGG - chr2_3:54171825-54171844 None:intergenic 25.0%
! AATTTAGGGAAACTTGTATA+TGG - chr2_3:54172102-54172121 None:intergenic 25.0%
! ATCATCTTCAATGTTACAAT+AGG - chr2_3:54172661-54172680 None:intergenic 25.0%
!! CTTTGAATTTTCTTATGCTT+TGG + chr2_3:54172858-54172877 Msa0299340:intron 25.0%
! CAATCAAAACATAATAGCAT+AGG + chr2_3:54172911-54172930 Msa0299340:intron 25.0%
!!! AAATCAGAGTTTTGATTTTG+TGG + chr2_3:54173172-54173191 Msa0299340:intron 25.0%
!!! AATCAGAGTTTTGATTTTGT+GGG + chr2_3:54173173-54173192 Msa0299340:intron 25.0%
! TCATTGGGAATATTTGTATA+GGG - chr2_3:54173412-54173431 None:intergenic 25.0%
! TTCATTGGGAATATTTGTAT+AGG - chr2_3:54173413-54173432 None:intergenic 25.0%
! CTGTATATGTTTGTTTCATT+GGG - chr2_3:54173427-54173446 None:intergenic 25.0%
! ACTGTATATGTTTGTTTCAT+TGG - chr2_3:54173428-54173447 None:intergenic 25.0%
!! TCTACACTTTTCTATATTCA+GGG - chr2_3:54173538-54173557 None:intergenic 25.0%
!! TTCTACACTTTTCTATATTC+AGG - chr2_3:54173539-54173558 None:intergenic 25.0%
! TCATGGATATTGAATATGTT+GGG - chr2_3:54173582-54173601 None:intergenic 25.0%
! TTCATGGATATTGAATATGT+TGG - chr2_3:54173583-54173602 None:intergenic 25.0%
!!! TTCTACTAAGTCTATTTTGA+TGG - chr2_3:54173699-54173718 None:intergenic 25.0%
! AAATAGACTTAGTAGAAATC+AGG + chr2_3:54173702-54173721 Msa0299340:intron 25.0%
!!! CTAGTTTTTTTACTGAAAGA+AGG - chr2_3:54173871-54173890 None:intergenic 25.0%
!!! AGATGTTTTCAGGAATTTTT+TGG + chr2_3:54174098-54174117 Msa0299340:intron 25.0%
!! TTATATGAGGCATCATATTT+AGG + chr2_3:54174177-54174196 Msa0299340:CDS 25.0%
!! TATATGAGGCATCATATTTA+GGG + chr2_3:54174178-54174197 Msa0299340:CDS 25.0%
! TCATAATTTCACACATTCAT+CGG + chr2_3:54174549-54174568 Msa0299340:intron 25.0%
!!! CGGTTGCTAATTTAATTTTT+TGG - chr2_3:54174760-54174779 None:intergenic 25.0%
! TTCATGGCAGAAATTAATAT+TGG - chr2_3:54174877-54174896 None:intergenic 25.0%
!!! GTTTATTTGATTTGATTAGG+TGG + chr2_3:54174963-54174982 Msa0299340:intron 25.0%
! CATAACTAGCAAAATACATA+TGG - chr2_3:54175116-54175135 None:intergenic 25.0%
!! CATATGTATTTTGCTAGTTA+TGG + chr2_3:54175114-54175133 Msa0299340:CDS 25.0%
!! ACATAAAGTTTAGTTTAGCA+TGG - chr2_3:54175273-54175292 None:intergenic 25.0%
! ATACATGAAGATATGCTATA+TGG + chr2_3:54175333-54175352 Msa0299340:CDS 25.0%
! TCTATATGCAATTTCATTTG+TGG - chr2_3:54175371-54175390 None:intergenic 25.0%
! TATAGAATTCAGAAAGAACA+TGG + chr2_3:54175385-54175404 Msa0299340:CDS 25.0%
! ATTATTAACATGTCACTCTT+TGG - chr2_3:54175486-54175505 None:intergenic 25.0%
! CATGTTAATAATTGTAGACT+TGG + chr2_3:54175494-54175513 Msa0299340:intron 25.0%
!!! GCCAAATTTTAAGACATATT+TGG + chr2_3:54175592-54175611 Msa0299340:CDS 25.0%
!! AATTTGTTTCATAGGAATCA+TGG - chr2_3:54176344-54176363 None:intergenic 25.0%
!! TACTAATTTTTATGTTCAGG+AGG + chr2_3:54176405-54176424 Msa0299340:intron 25.0%
! ATGAAGGAAGAATACAAATA+AGG + chr2_3:54176497-54176516 Msa0299340:CDS 25.0%
!!! TCTTCAACTCAATTTTTAGT+AGG - chr2_3:54176706-54176725 None:intergenic 25.0%
! ACATTTGCTCGTTGTAATTT+TGG + chr2_3:54171147-54171166 Msa0299340:CDS 30.0%
! CATTTGCTCGTTGTAATTTT+GGG + chr2_3:54171148-54171167 Msa0299340:CDS 30.0%
CGCTACAAAAATCAAATTCA+AGG + chr2_3:54171199-54171218 Msa0299340:CDS 30.0%
TCAAATTCAAGGATTATGCA+TGG + chr2_3:54171210-54171229 Msa0299340:CDS 30.0%
AAAATAAAGGGTTATGCTAG+AGG + chr2_3:54171750-54171769 Msa0299340:intron 30.0%
GTCTTATCTTCAGTTTGTAA+AGG - chr2_3:54172580-54172599 None:intergenic 30.0%
TGAAGATAAGACCAAAAGTT+TGG + chr2_3:54172588-54172607 Msa0299340:CDS 30.0%
GAAGATAAGACCAAAAGTTT+GGG + chr2_3:54172589-54172608 Msa0299340:CDS 30.0%
TTGAATTTAGCAATGCTTCT+CGG - chr2_3:54172633-54172652 None:intergenic 30.0%
ATGAGATCAATGTTCAACTT+GGG + chr2_3:54172722-54172741 Msa0299340:CDS 30.0%
ATACATGAATGCAATATACC+AGG - chr2_3:54172835-54172854 None:intergenic 30.0%
AGAGGTCTAGGTTTATATAT+GGG - chr2_3:54173051-54173070 None:intergenic 30.0%
TAGAGGTCTAGGTTTATATA+TGG - chr2_3:54173052-54173071 None:intergenic 30.0%
!!! ATCAGAGTTTTGATTTTGTG+GGG + chr2_3:54173174-54173193 Msa0299340:intron 30.0%
! CACTTAAGTGATATACCTTT+TGG - chr2_3:54173207-54173226 None:intergenic 30.0%
ATATCACTTAAGTGAGTGTT+TGG + chr2_3:54173213-54173232 Msa0299340:intron 30.0%
!! TAATGACTTGTTTGGTTATG+CGG - chr2_3:54173358-54173377 None:intergenic 30.0%
!!! GTTTTGACTAATGACTTGTT+TGG - chr2_3:54173366-54173385 None:intergenic 30.0%
ATTGGGAATATTTGTATAGG+GGG - chr2_3:54173410-54173429 None:intergenic 30.0%
CATTGGGAATATTTGTATAG+GGG - chr2_3:54173411-54173430 None:intergenic 30.0%
CATGGATATTGAATATGTTG+GGG - chr2_3:54173581-54173600 None:intergenic 30.0%
TAATTCACTTAATGGCTTCA+TGG - chr2_3:54173599-54173618 None:intergenic 30.0%
TACATGACATTATGACCTTA+TGG + chr2_3:54173623-54173642 Msa0299340:intron 30.0%
!! ATTTTGATGGCTAAATGCAA+GGG - chr2_3:54173686-54173705 None:intergenic 30.0%
!!! TATTTTGATGGCTAAATGCA+AGG - chr2_3:54173687-54173706 None:intergenic 30.0%
!! TTTTACTGAAAGAAGGTACA+AGG - chr2_3:54173864-54173883 None:intergenic 30.0%
CCAATAATACATGTCAGTAT+AGG - chr2_3:54173926-54173945 None:intergenic 30.0%
CCTATACTGACATGTATTAT+TGG + chr2_3:54173923-54173942 Msa0299340:intron 30.0%
! ACTTGATGATTTGATTAGTG+TGG + chr2_3:54174063-54174082 Msa0299340:intron 30.0%
ATTTGATTAGTGTGGTTGTT+TGG + chr2_3:54174071-54174090 Msa0299340:intron 30.0%
AATCACTTACCAATGACATT+TGG + chr2_3:54174142-54174161 Msa0299340:CDS 30.0%
ATCACTTACCAATGACATTT+GGG + chr2_3:54174143-54174162 Msa0299340:CDS 30.0%
! AAAAAGCCATGGAATTTTCA+AGG + chr2_3:54174223-54174242 Msa0299340:CDS 30.0%
! AAAAGCCATGGAATTTTCAA+GGG + chr2_3:54174224-54174243 Msa0299340:CDS 30.0%
! TAACCAAATACCAACTCTTT+TGG + chr2_3:54174383-54174402 Msa0299340:CDS 30.0%
!! ATTCCAAAAGAGTTGGTATT+TGG - chr2_3:54174389-54174408 None:intergenic 30.0%
!!! AATACCAACTCTTTTGGAAT+TGG + chr2_3:54174389-54174408 Msa0299340:CDS 30.0%
! ATATTTCTGCCAATTCTCTT+TGG - chr2_3:54174449-54174468 None:intergenic 30.0%
AAAGGTGGAAGAAATTGATT+TGG + chr2_3:54174515-54174534 Msa0299340:intron 30.0%
! AGTAGACAAAAAGAAGTATG+AGG - chr2_3:54174909-54174928 None:intergenic 30.0%
!! TATTTGATTTGATTAGGTGG+TGG + chr2_3:54174966-54174985 Msa0299340:intron 30.0%
! TGGTTTTTGTAAGTTCAATG+CGG - chr2_3:54175096-54175115 None:intergenic 30.0%
GATGATATCTTTGACACTTA+TGG + chr2_3:54175136-54175155 Msa0299340:CDS 30.0%
!! TGAACTTGTTCTTTTCACAA+AGG + chr2_3:54175168-54175187 Msa0299340:CDS 30.0%
! TTTTCACAAAGGCAATCAAA+AGG + chr2_3:54175179-54175198 Msa0299340:CDS 30.0%
TAGCATATCTTCATGTATTC+AGG - chr2_3:54175331-54175350 None:intergenic 30.0%
! AGATTCTTACTGTTCTTTTC+AGG - chr2_3:54175429-54175448 None:intergenic 30.0%
CATTTCTTGAAGAAGCTAAA+TGG + chr2_3:54175552-54175571 Msa0299340:CDS 30.0%
GAAGCTAAATGGTTCAATAA+TGG + chr2_3:54175563-54175582 Msa0299340:CDS 30.0%
ATTCTTGTAGACATCATCAA+AGG + chr2_3:54176117-54176136 Msa0299340:intron 30.0%
ATACAAATAAGGATGCTACT+AGG + chr2_3:54176508-54176527 Msa0299340:CDS 30.0%
TACAAATAAGGATGCTACTA+GGG + chr2_3:54176509-54176528 Msa0299340:CDS 30.0%
TATCAAAGCTTCTCTTAACA+TGG + chr2_3:54176588-54176607 Msa0299340:CDS 30.0%
GCTCAAGTTATTTATCAACA+TGG + chr2_3:54176619-54176638 Msa0299340:CDS 30.0%
AGTTTGTGGAATGTTGTAGT+TGG + chr2_3:54171430-54171449 Msa0299340:intron 35.0%
AGAAGATGCTAAAAGACACT+TGG - chr2_3:54171727-54171746 None:intergenic 35.0%
TGAGACAAAAAGATGTGTTG+TGG - chr2_3:54171907-54171926 None:intergenic 35.0%
GTGTGAGTGCTACATTTAAT+TGG + chr2_3:54172146-54172165 Msa0299340:intron 35.0%
TTCTGAACTCAACTCTTACA+CGG - chr2_3:54172173-54172192 None:intergenic 35.0%
TAGTAAAACAAAACCAAGCC+AGG - chr2_3:54172227-54172246 None:intergenic 35.0%
! ATTTTCTCCACATGTGTTGT+AGG - chr2_3:54172262-54172281 None:intergenic 35.0%
!!! TTTGACTTGACCCAAACTTT+TGG - chr2_3:54172602-54172621 None:intergenic 35.0%
! AAGATGATTGACTCAATCCA+AGG + chr2_3:54172673-54172692 Msa0299340:CDS 35.0%
! AGATGATTGACTCAATCCAA+GGG + chr2_3:54172674-54172693 Msa0299340:CDS 35.0%
CATTGATCTCATCTTCCAAA+TGG - chr2_3:54172714-54172733 None:intergenic 35.0%
GATGAGATCAATGTTCAACT+TGG + chr2_3:54172721-54172740 Msa0299340:CDS 35.0%
ACTCTCTTGACATGACAAAA+TGG - chr2_3:54172885-54172904 None:intergenic 35.0%
GTTCTAATATGTAGAGGTCT+AGG - chr2_3:54173063-54173082 None:intergenic 35.0%
!!! TCAGAGTTTTGATTTTGTGG+GGG + chr2_3:54173175-54173194 Msa0299340:intron 35.0%
! TGAGTGTTTGGATCAACTTT+TGG + chr2_3:54173225-54173244 Msa0299340:intron 35.0%
TTCCACGTTGAAATTGCATT+GGG - chr2_3:54173267-54173286 None:intergenic 35.0%
AGCTCATATTTGTAGCTTCA+TGG - chr2_3:54173290-54173309 None:intergenic 35.0%
AGCTTCATCTTGTATCCATA+AGG - chr2_3:54173641-54173660 None:intergenic 35.0%
AATACATGTCAGTATAGGTG+TGG - chr2_3:54173921-54173940 None:intergenic 35.0%
! GTTTGGTTGTAGATGTTTTC+AGG + chr2_3:54174088-54174107 Msa0299340:intron 35.0%
!!! AGGAATTTTTTGGACAAGAG+TGG + chr2_3:54174108-54174127 Msa0299340:CDS 35.0%
TCACTTACCAATGACATTTG+GGG + chr2_3:54174144-54174163 Msa0299340:CDS 35.0%
GGGTATGTTGAGTTTATATG+AGG + chr2_3:54174164-54174183 Msa0299340:CDS 35.0%
TTTGCCAATTCCAAAAGAGT+TGG - chr2_3:54174396-54174415 None:intergenic 35.0%
! ATTCTCTTTGGTGTAATGAC+TGG - chr2_3:54174437-54174456 None:intergenic 35.0%
GTCATTACACCAAAGAGAAT+TGG + chr2_3:54174437-54174456 Msa0299340:CDS 35.0%
ACACATTCATCGGTAGTAAT+AGG + chr2_3:54174559-54174578 Msa0299340:intron 35.0%
TTTGTCTATTCTCGATCCAA+TGG + chr2_3:54174659-54174678 Msa0299340:intron 35.0%
ATAAATGTCACGCATTCACA+TGG - chr2_3:54174693-54174712 None:intergenic 35.0%
TTCCTTGTCGCTAAAATTAG+TGG - chr2_3:54174824-54174843 None:intergenic 35.0%
TATGAGGTTTGTGATGTTCA+TGG - chr2_3:54174893-54174912 None:intergenic 35.0%
! TTTTGCAAGAGATAGACCAA+CGG + chr2_3:54175018-54175037 Msa0299340:CDS 35.0%
CAATTTGAATGACATGGTTC+TGG - chr2_3:54175073-54175092 None:intergenic 35.0%
!!! TCTTTTCAGGTGAGAAACAA+CGG - chr2_3:54175416-54175435 None:intergenic 35.0%
TGTCACTCTTTGGTATACTT+TGG - chr2_3:54175476-54175495 None:intergenic 35.0%
!! TTGGTGATTAATTTGTGCAG+TGG + chr2_3:54175513-54175532 Msa0299340:intron 35.0%
AATTTGTGGAGAGAACTCAA+TGG + chr2_3:54176532-54176551 Msa0299340:CDS 35.0%
!! AACAGACGAAGGTATGTTTT+TGG - chr2_3:54176569-54176588 None:intergenic 35.0%
AGGTAATGATGTGAAGATCA+AGG - chr2_3:54176686-54176705 None:intergenic 35.0%
AGATACAGATTCTATGCCTA+TGG + chr2_3:54176725-54176744 Msa0299340:three_prime_UTR 35.0%
GCCTGCAAATACAATGACAA+TGG + chr2_3:54171089-54171108 Msa0299340:exon 40.0%
GCCATTGTCATTGTATTTGC+AGG - chr2_3:54171093-54171112 None:intergenic 40.0%
TACCTTCTCATTGAGAGCAA+TGG - chr2_3:54171253-54171272 None:intergenic 40.0%
GATGCTAAAAGACACTTGGT+AGG - chr2_3:54171723-54171742 None:intergenic 40.0%
! AGTAACTAATTTTGCGGCCT+AGG + chr2_3:54172124-54172143 Msa0299340:intron 40.0%
TAAATGTAGCACTCACACCT+AGG - chr2_3:54172144-54172163 None:intergenic 40.0%
TAAAACAAAACCAAGCCAGG+TGG - chr2_3:54172224-54172243 None:intergenic 40.0%
GAGAATTCCTACAACACATG+TGG + chr2_3:54172252-54172271 Msa0299340:intron 40.0%
AGATCAATGTTCAACTTGGG+AGG + chr2_3:54172725-54172744 Msa0299340:CDS 40.0%
AACTTGGGAGGATATGTGAT+TGG + chr2_3:54172737-54172756 Msa0299340:CDS 40.0%
ACTTGGGAGGATATGTGATT+GGG + chr2_3:54172738-54172757 Msa0299340:CDS 40.0%
TTGCCACATCCCTTCAATTT+CGG + chr2_3:54172779-54172798 Msa0299340:CDS 40.0%
TAACCGAAATTGAAGGGATG+TGG - chr2_3:54172785-54172804 None:intergenic 40.0%
CCTCAGTAACCGAAATTGAA+GGG - chr2_3:54172791-54172810 None:intergenic 40.0%
TTTCGGTTACTGAGGCATAA+TGG + chr2_3:54172796-54172815 Msa0299340:CDS 40.0%
TGCAATATACCAGGACATGT+GGG - chr2_3:54172826-54172845 None:intergenic 40.0%
ATGCAATATACCAGGACATG+TGG - chr2_3:54172827-54172846 None:intergenic 40.0%
GACCTCTACATATTAGAACG+TGG + chr2_3:54173064-54173083 Msa0299340:intron 40.0%
CTCCACGTTCTAATATGTAG+AGG - chr2_3:54173069-54173088 None:intergenic 40.0%
GTTCCACGTTGAAATTGCAT+TGG - chr2_3:54173268-54173287 None:intergenic 40.0%
TGGATACAAGATGAAGCTCT+AGG + chr2_3:54173643-54173662 Msa0299340:intron 40.0%
AATGCAAGGGTGGAAACATT+GGG - chr2_3:54173673-54173692 None:intergenic 40.0%
AAATGCAAGGGTGGAAACAT+TGG - chr2_3:54173674-54173693 None:intergenic 40.0%
! TGGACAAGAGTGGCAATTTT+AGG + chr2_3:54174118-54174137 Msa0299340:CDS 40.0%
! GGACAAGAGTGGCAATTTTA+GGG + chr2_3:54174119-54174138 Msa0299340:CDS 40.0%
CAACATACCCCAAATGTCAT+TGG - chr2_3:54174154-54174173 None:intergenic 40.0%
!! CATTCTTAGGTGCTTTGGAA+GGG - chr2_3:54174316-54174335 None:intergenic 40.0%
CTTCTAACCTTGCCATTCTT+AGG - chr2_3:54174329-54174348 None:intergenic 40.0%
GAGAATTGGCAGAAATATGC+AGG + chr2_3:54174451-54174470 Msa0299340:CDS 40.0%
!! TGCCACTAATTTTAGCGACA+AGG + chr2_3:54174819-54174838 Msa0299340:intron 40.0%
!! ATTAGGTGGTGGAAAGAGTT+GGG + chr2_3:54174977-54174996 Msa0299340:intron 40.0%
!! TTGGGACTTGTTGACAAACT+TGG + chr2_3:54174995-54175014 Msa0299340:CDS 40.0%
AGTGCTTCTTATGGACAGTA+GGG + chr2_3:54175041-54175060 Msa0299340:CDS 40.0%
ATGCGGCAATTTGAATGACA+TGG - chr2_3:54175079-54175098 None:intergenic 40.0%
CACAAAGGCAATCAAAAGGT+TGG + chr2_3:54175183-54175202 Msa0299340:intron 40.0%
!! GATAATGGAGTGATTTCTGC+AGG + chr2_3:54175614-54175633 Msa0299340:CDS 40.0%
GATTTCTGCAGGATCATGTA+TGG + chr2_3:54175625-54175644 Msa0299340:CDS 40.0%
GGGATATGACTTCATCATCA+TGG - chr2_3:54175705-54175724 None:intergenic 40.0%
! TGATGATGAAGTCATATCCC+AGG + chr2_3:54175705-54175724 Msa0299340:CDS 40.0%
GGATGACTTAGGAACTTCAA+CGG + chr2_3:54175763-54175782 Msa0299340:CDS 40.0%
!!! CGAAGGTATGTTTTTGGTCA+TGG - chr2_3:54176563-54176582 None:intergenic 40.0%
! GAAGCTTTGATAACAGACGA+AGG - chr2_3:54176580-54176599 None:intergenic 40.0%
GTCCATTGCTCTCAATGAGA+AGG + chr2_3:54171248-54171267 Msa0299340:CDS 45.0%
TAGAGTGAATTGCAACCACC+TGG + chr2_3:54172206-54172225 Msa0299340:intron 45.0%
! TGGGCAGCAGCAACATTTTA+CGG + chr2_3:54172340-54172359 Msa0299340:intron 45.0%
! ATTGACTCAATCCAAGGGCT+GGG + chr2_3:54172679-54172698 Msa0299340:CDS 45.0%
TGGCAAAGAGGTCTTGAGAA+GGG - chr2_3:54172765-54172784 None:intergenic 45.0%
ATTGAAGGGATGTGGCAAAG+AGG - chr2_3:54172777-54172796 None:intergenic 45.0%
CCCTTCAATTTCGGTTACTG+AGG + chr2_3:54172788-54172807 Msa0299340:CDS 45.0%
GCCTCAGTAACCGAAATTGA+AGG - chr2_3:54172792-54172811 None:intergenic 45.0%
TTGTGGGGGACAAAACCAAA+AGG + chr2_3:54173189-54173208 Msa0299340:intron 45.0%
GTCCCAATGCAATTTCAACG+TGG + chr2_3:54173262-54173281 Msa0299340:intron 45.0%
ATGCAAGGGTGGAAACATTG+GGG - chr2_3:54173672-54173691 None:intergenic 45.0%
! TTGATGGCTAAATGCAAGGG+TGG - chr2_3:54173683-54173702 None:intergenic 45.0%
ACGAGCCCTTGAAAATTCCA+TGG - chr2_3:54174232-54174251 None:intergenic 45.0%
CTACTTCAGGACTAAGGTGT+GGG - chr2_3:54174269-54174288 None:intergenic 45.0%
!! TAGGTGCTTTGGAAGGGTTA+AGG - chr2_3:54174310-54174329 None:intergenic 45.0%
! CCATTCTTAGGTGCTTTGGA+AGG - chr2_3:54174317-54174336 None:intergenic 45.0%
! CCTTCCAAAGCACCTAAGAA+TGG + chr2_3:54174314-54174333 Msa0299340:CDS 45.0%
CTTGCCATTCTTAGGTGCTT+TGG - chr2_3:54174321-54174340 None:intergenic 45.0%
! CAAAGCACCTAAGAATGGCA+AGG + chr2_3:54174319-54174338 Msa0299340:CDS 45.0%
TCACATGGTCAAGAGACCAT+TGG - chr2_3:54174678-54174697 None:intergenic 45.0%
!! GATTAGGTGGTGGAAAGAGT+TGG + chr2_3:54174976-54174995 Msa0299340:intron 45.0%
GAGTGCTTCTTATGGACAGT+AGG + chr2_3:54175040-54175059 Msa0299340:CDS 45.0%
GAAGTTGCTCCATTGCATCA+AGG - chr2_3:54175309-54175328 None:intergenic 45.0%
TTGAGAGACCATCACCAATG+AGG - chr2_3:54175670-54175689 None:intergenic 45.0%
!! CATTGGTGATGGTCTCTCAA+AGG + chr2_3:54175670-54175689 Msa0299340:CDS 45.0%
CCATAGTCGAAGAATTTCCC+CGG - chr2_3:54175744-54175763 None:intergenic 45.0%
CGGGGAAATTCTTCGACTAT+GGG + chr2_3:54175742-54175761 Msa0299340:CDS 45.0%
CTTCGACTATGGGATGACTT+AGG + chr2_3:54175752-54175771 Msa0299340:CDS 45.0%
! GTCATGCTAACTTGTGCTCT+GGG + chr2_3:54175828-54175847 Msa0299340:intron 45.0%
! TCATGCTAACTTGTGCTCTG+GGG + chr2_3:54175829-54175848 Msa0299340:intron 45.0%
GTTCAGGAGGAACAAGAAAG+AGG + chr2_3:54176418-54176437 Msa0299340:intron 45.0%
GGATGCTACTAGGGAATTTG+TGG + chr2_3:54176518-54176537 Msa0299340:CDS 45.0%
!!! ATTTCATATATTTAATTTAA+CGG - chr2_3:54173028-54173047 None:intergenic 5.0%
!! GAAAATTAATTAATAAATAA+AGG + chr2_3:54175805-54175824 Msa0299340:intron 5.0%
!!! AATTTAAATATTTTTATTAG+TGG + chr2_3:54176015-54176034 Msa0299340:intron 5.0%
TGAATTGCAACCACCTGGCT+TGG + chr2_3:54172211-54172230 Msa0299340:intron 50.0%
! GATTGACTCAATCCAAGGGC+TGG + chr2_3:54172678-54172697 Msa0299340:CDS 50.0%
TCAATCCAAGGGCTGGGAAT+TGG + chr2_3:54172685-54172704 Msa0299340:CDS 50.0%
GCTGGGAATTGGTCACCATT+TGG + chr2_3:54172696-54172715 Msa0299340:CDS 50.0%
GTGGCAAAGAGGTCTTGAGA+AGG - chr2_3:54172766-54172785 None:intergenic 50.0%
GGTTACTGAGGCATAATGGC+TGG + chr2_3:54172800-54172819 Msa0299340:CDS 50.0%
CCTACTTCAGGACTAAGGTG+TGG - chr2_3:54174270-54174289 None:intergenic 50.0%
CCACACCTTAGTCCTGAAGT+AGG + chr2_3:54174267-54174286 Msa0299340:CDS 50.0%
ACCTTAGTCCTGAAGTAGGC+AGG + chr2_3:54174271-54174290 Msa0299340:CDS 50.0%
CCAATTTGCCTGCCTACTTC+AGG - chr2_3:54174282-54174301 None:intergenic 50.0%
CCTGAAGTAGGCAGGCAAAT+TGG + chr2_3:54174279-54174298 Msa0299340:CDS 50.0%
! GACCAACGGAGTGCTTCTTA+TGG + chr2_3:54175032-54175051 Msa0299340:CDS 50.0%
!! GTCCATAAGAAGCACTCCGT+TGG - chr2_3:54175037-54175056 None:intergenic 50.0%
CAGATGGGACCTTGATGCAA+TGG + chr2_3:54175297-54175316 Msa0299340:intron 50.0%
ATGCATGCCACCTTCCTCAT+TGG + chr2_3:54175653-54175672 Msa0299340:CDS 50.0%
ACCATCACCAATGAGGAAGG+TGG - chr2_3:54175663-54175682 None:intergenic 50.0%
GAGACCATCACCAATGAGGA+AGG - chr2_3:54175666-54175685 None:intergenic 50.0%
CCGGGGAAATTCTTCGACTA+TGG + chr2_3:54175741-54175760 Msa0299340:CDS 50.0%
! GGTCATGCTAACTTGTGCTC+TGG + chr2_3:54175827-54175846 Msa0299340:intron 50.0%
GCCTGCCTACTTCAGGACTA+AGG - chr2_3:54174275-54174294 None:intergenic 55.0%
GCCACCTTCCTCATTGGTGA+TGG + chr2_3:54175659-54175678 Msa0299340:CDS 55.0%
GGTGACCAATTCCCAGCCCT+TGG - chr2_3:54172693-54172712 None:intergenic 60.0%
! AATGGCTGGCCCACATGTCC+TGG + chr2_3:54172814-54172833 Msa0299340:CDS 60.0%
CCGGAGCAAGTGAAGAGCCT+GGG - chr2_3:54175725-54175744 None:intergenic 60.0%
CCCAGGCTCTTCACTTGCTC+CGG + chr2_3:54175722-54175741 Msa0299340:CDS 60.0%
CCAGGCTCTTCACTTGCTCC+GGG + chr2_3:54175723-54175742 Msa0299340:CDS 60.0%
CAGGCTCTTCACTTGCTCCG+GGG + chr2_3:54175724-54175743 Msa0299340:CDS 60.0%
CCCGGAGCAAGTGAAGAGCC+TGG - chr2_3:54175726-54175745 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 54171065 54176877 54171065 ID=Msa0299340;Name=Msa0299340
chr2_3 mRNA 54171065 54176877 54171065 ID=Msa0299340-mRNA-1;Parent=Msa0299340;Name=Msa0299340-mRNA-1;_AED=0.04;_eAED=0.04;_QI=37|1|1|1|1|1|7|160|569
chr2_3 exon 54171065 54171269 54171065 ID=Msa0299340-mRNA-1:exon:17286;Parent=Msa0299340-mRNA-1
chr2_3 exon 54172571 54172835 54172571 ID=Msa0299340-mRNA-1:exon:17287;Parent=Msa0299340-mRNA-1
chr2_3 exon 54174100 54174472 54174100 ID=Msa0299340-mRNA-1:exon:17288;Parent=Msa0299340-mRNA-1
chr2_3 exon 54174982 54175200 54174982 ID=Msa0299340-mRNA-1:exon:17289;Parent=Msa0299340-mRNA-1
chr2_3 exon 54175300 54175438 54175300 ID=Msa0299340-mRNA-1:exon:17290;Parent=Msa0299340-mRNA-1
chr2_3 exon 54175533 54175784 54175533 ID=Msa0299340-mRNA-1:exon:17291;Parent=Msa0299340-mRNA-1
chr2_3 exon 54176424 54176877 54176424 ID=Msa0299340-mRNA-1:exon:17292;Parent=Msa0299340-mRNA-1
chr2_3 five_prime_UTR 54171065 54171101 54171065 ID=Msa0299340-mRNA-1:five_prime_utr;Parent=Msa0299340-mRNA-1
chr2_3 CDS 54171102 54171269 54171102 ID=Msa0299340-mRNA-1:cds;Parent=Msa0299340-mRNA-1
chr2_3 CDS 54172571 54172835 54172571 ID=Msa0299340-mRNA-1:cds;Parent=Msa0299340-mRNA-1
chr2_3 CDS 54174100 54174472 54174100 ID=Msa0299340-mRNA-1:cds;Parent=Msa0299340-mRNA-1
chr2_3 CDS 54174982 54175200 54174982 ID=Msa0299340-mRNA-1:cds;Parent=Msa0299340-mRNA-1
chr2_3 CDS 54175300 54175438 54175300 ID=Msa0299340-mRNA-1:cds;Parent=Msa0299340-mRNA-1
chr2_3 CDS 54175533 54175784 54175533 ID=Msa0299340-mRNA-1:cds;Parent=Msa0299340-mRNA-1
chr2_3 CDS 54176424 54176717 54176424 ID=Msa0299340-mRNA-1:cds;Parent=Msa0299340-mRNA-1
chr2_3 three_prime_UTR 54176718 54176877 54176718 ID=Msa0299340-mRNA-1:three_prime_utr;Parent=Msa0299340-mRNA-1
Gene Sequence

>Msa0299340

ATGACAATGGCTGTTTCTGAACTTAGAGTTTCATATCTAACAAGCACATTTGCTCGTTGTAATTTTGGGACTTCTACATATCAATCACGCATCTTATCGCTACAAAAATCAAATTCAAGGATTATGCATGGTAAATTTTCGATCAAGTCCATTGCTCTCAATGAGAAGTTGTCACCTTTACAAACTGAAGATAAGACCAAAAGTTTGGGTCAAGTCAAAAGAAGAAGCCGAGAAGCATTGCTAAATTCAAATGATCCTATTGTAACATTGAAGATGATTGACTCAATCCAAGGGCTGGGAATTGGTCACCATTTGGAAGATGAGATCAATGTTCAACTTGGGAGGATATGTGATTGGGACCCTTCTCAAGACCTCTTTGCCACATCCCTTCAATTTCGGTTACTGAGGCATAATGGCTGGCCCACATGTCCTGATGTTTTCAGGAATTTTTTGGACAAGAGTGGCAATTTTAGGGAATCACTTACCAATGACATTTGGGGTATGTTGAGTTTATATGAGGCATCATATTTAGGGACAGAAGATGAAGAAATATTAAAAAAAGCCATGGAATTTTCAAGGGCTCGTCTAAGTGAATTAATCCCACACCTTAGTCCTGAAGTAGGCAGGCAAATTGGAAGAGCCTTAACCCTTCCAAAGCACCTAAGAATGGCAAGGTTAGAAGCTAGAAACTACATTGAAGAATATAGTCAAGAAAGTAACCAAATACCAACTCTTTTGGAATTGGCAAAGTTAGACTCTGAGATGATCCAGTCATTACACCAAAGAGAATTGGCAGAAATATGCAGGTGGTGGAAAGAGTTGGGACTTGTTGACAAACTTGGTTTTGCAAGAGATAGACCAACGGAGTGCTTCTTATGGACAGTAGGGATATTTCCAGAACCATGTCATTCAAATTGCCGCATTGAACTTACAAAAACCATATGTATTTTGCTAGTTATGGATGATATCTTTGACACTTATGGCACATTAGATGAACTTGTTCTTTTCACAAAGGCAATCAAAAGATGGGACCTTGATGCAATGGAGCAACTTCCTGAATACATGAAGATATGCTATATGGCATTATATAACACCACAAATGAAATTGCATATAGAATTCAGAAAGAACATGGACTAACCGTTGTTTCTCACCTGAAAAGAACATGGATTGACATATTTGAAGCATTTCTTGAAGAAGCTAAATGGTTCAATAATGGAAGTGTGCCAAATTTTAAGACATATTTGGATAATGGAGTGATTTCTGCAGGATCATGTATGGCATTGATGCATGCCACCTTCCTCATTGGTGATGGTCTCTCAAAGGAAACTATTTCCATGATGATGAAGTCATATCCCAGGCTCTTCACTTGCTCCGGGGAAATTCTTCGACTATGGGATGACTTAGGAACTTCAACGGAGGAACAAGAAAGAGGAGATAATGCTAGCAGTATACAATGCATGATGAGAGAGAATAATATTTCAGATGAAAATGAAGGAAGAATACAAATAAGGATGCTACTAGGGAATTTGTGGAGAGAACTCAATGGTCTTGCCATGACCAAAAACATACCTTCGTCTGTTATCAAAGCTTCTCTTAACATGGCTAGAACTGCTCAAGTTATTTATCAACATGGAGATGATCAAAGCACATTTACTGTTGATGACTATGTGCAAACCTTGATCTTCACATCATTACCTACTAAAAATTGA
Protein sequence

>Msa0299340

MTMAVSELRVSYLTSTFARCNFGTSTYQSRILSLQKSNSRIMHGKFSIKSIALNEKLSPLQTEDKTKSLGQVKRRSREALLNSNDPIVTLKMIDSIQGLGIGHHLEDEINVQLGRICDWDPSQDLFATSLQFRLLRHNGWPTCPDVFRNFLDKSGNFRESLTNDIWGMLSLYEASYLGTEDEEILKKAMEFSRARLSELIPHLSPEVGRQIGRALTLPKHLRMARLEARNYIEEYSQESNQIPTLLELAKLDSEMIQSLHQRELAEICRWWKELGLVDKLGFARDRPTECFLWTVGIFPEPCHSNCRIELTKTICILLVMDDIFDTYGTLDELVLFTKAIKRWDLDAMEQLPEYMKICYMALYNTTNEIAYRIQKEHGLTVVSHLKRTWIDIFEAFLEEAKWFNNGSVPNFKTYLDNGVISAGSCMALMHATFLIGDGLSKETISMMMKSYPRLFTCSGEILRLWDDLGTSTEEQERGDNASSIQCMMRENNISDENEGRIQIRMLLGNLWRELNGLAMTKNIPSSVIKASLNMARTAQVIYQHGDDQSTFTVDDYVQTLIFTSLPTKN*