AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0261230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261230 MtrunA17_Chr2g0323271 88.841 233 21 2 1 228 1 233 1.26e-122 347
Msa0261230 MtrunA17_Chr2g0280901 49.296 142 47 2 1 142 1 117 7.77e-32 112
Msa0261230 MtrunA17_Chr2g0322971 52.542 59 28 0 3 61 29 87 1.40e-13 65.5
Msa0261230 MtrunA17_Chr4g0024021 50.847 59 29 0 5 63 36 94 9.26e-13 63.2
Msa0261230 MtrunA17_Chr4g0002211 50.000 66 33 0 3 68 6 71 2.10e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261230 AT4G27310.1 43.103 174 51 4 1 136 1 164 8.71e-36 126
Msa0261230 AT5G54470.1 45.946 185 81 4 2 186 3 168 2.48e-34 122
Msa0261230 AT3G21890.1 56.000 50 22 0 5 54 31 80 8.35e-11 58.2

Find 71 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTTTCTTCTTGTTGCT+CGG 0.346480 2_2:-59500608 None:intergenic
CGGCGGTATAGCGATGTAAA+TGG 0.352357 2_2:+59501083 Msa0261230:CDS
TTCTTCTTGTTGCTCGGCGC+TGG 0.356031 2_2:-59500602 None:intergenic
CTCATCTTCCTCATCGGAAT+CGG 0.374286 2_2:-59500656 None:intergenic
GTGGATGAGGTTGATTTGAT+AGG 0.378300 2_2:+59501026 Msa0261230:CDS
ACGAAACCGCGTTGAGAGAA+AGG 0.398885 2_2:+59500294 Msa0261230:five_prime_UTR
TGAAGAAGATGGTGATAATC+AGG 0.403542 2_2:+59500687 Msa0261230:CDS
CGCATCGCCGCCTTTGACGA+CGG 0.405274 2_2:-59501062 None:intergenic
GATTATGGTTGTGGCCTTGT+GGG 0.422309 2_2:+59501289 Msa0261230:three_prime_UTR
CTCGGCGCTGGCACCGGCGT+TGG 0.429781 2_2:-59500590 None:intergenic
ATGCTGGGACTGCGATTCTA+AGG 0.430981 2_2:+59500405 Msa0261230:CDS
CCACCGGCTTCGAGTTCTTC+CGG 0.435713 2_2:+59500742 Msa0261230:CDS
TTTCTTGTGATGGTTTGGTA+TGG 0.436961 2_2:-59500188 None:intergenic
CGTGGAAAGCCTCCGGTGCT+AGG 0.445561 2_2:+59500500 Msa0261230:CDS
TTGTTGCTCGGCGCTGGCAC+CGG 0.453269 2_2:-59500596 None:intergenic
TGTTTATATAACTTAGGTAA+AGG 0.465810 2_2:+59501361 Msa0261230:three_prime_UTR
CCGACCAGGCTAGCTTATGC+TGG 0.471766 2_2:+59500389 Msa0261230:CDS
CGGTAGCGATGAGTCGGTTA+CGG 0.474621 2_2:+59500762 Msa0261230:CDS
GGTTTGGTATGGATGAAGAA+TGG 0.486262 2_2:-59500177 None:intergenic
GTTTGGTATGGATGAAGAAT+GGG 0.493335 2_2:-59500176 None:intergenic
CGCGTTACCGAGCCTAGCAC+CGG 0.516421 2_2:-59500512 None:intergenic
GCCAACGCTAACTCCAACGC+CGG 0.517678 2_2:+59500577 Msa0261230:CDS
ATAATATCTCTAATATAGTA+CGG 0.521383 2_2:-59501136 None:intergenic
CTCGTCCTCATCTTCCTCAT+CGG 0.523137 2_2:-59500662 None:intergenic
GACTCACAGAAAGTTCTAGC+TGG 0.526494 2_2:-59500367 None:intergenic
AGTCCCAGCATAAGCTAGCC+TGG 0.526991 2_2:-59500393 None:intergenic
ACCGGCGTTGGAGTTAGCGT+TGG 0.527253 2_2:-59500578 None:intergenic
GAGGACGAGGATGAAGAAGA+TGG 0.529527 2_2:+59500676 Msa0261230:CDS
AACTTTCTGTGAGTCCGACC+AGG 0.531925 2_2:+59500375 Msa0261230:CDS
ACCGGAGGCTTTCCACGGCG+TGG 0.539884 2_2:-59500494 None:intergenic
TGGATGAGGTTGATTTGATA+GGG 0.541461 2_2:+59501027 Msa0261230:CDS
CCCACGCCGTGGAAAGCCTC+CGG 0.541657 2_2:+59500493 Msa0261230:CDS
CGATGAGGAAGATGAGGACG+AGG 0.544127 2_2:+59500663 Msa0261230:CDS
CGACGAGGAACCGTCGTCAA+AGG 0.547277 2_2:+59501052 Msa0261230:CDS
GAAGAACTCGAAGCCGGTGG+TGG 0.549423 2_2:-59500739 None:intergenic
CGTCAAAGGCGGCGATGCGG+CGG 0.550222 2_2:+59501066 Msa0261230:CDS
AACAGCGGCGCAACCACCAC+CGG 0.553693 2_2:+59500726 Msa0261230:CDS
CGACCAGGCTAGCTTATGCT+GGG 0.557426 2_2:+59500390 Msa0261230:CDS
GGATTATGGTTGTGGCCTTG+TGG 0.566677 2_2:+59501288 Msa0261230:three_prime_UTR
CATTCTCAGCCACACGATTG+AGG 0.570452 2_2:+59501113 Msa0261230:CDS
CGTAACCGACTCATCGCTAC+CGG 0.571656 2_2:-59500761 None:intergenic
TCAACGAAGAGAGGTGGATG+AGG 0.575020 2_2:+59501013 Msa0261230:CDS
AGAATGGGTAAGAAGAGGAA+AGG 0.576671 2_2:-59500161 None:intergenic
TTCTTCCGGTAGCGATGAGT+CGG 0.577433 2_2:+59500756 Msa0261230:CDS
CGTTGGAGTTAGCGTTGGCG+TGG 0.586469 2_2:-59500573 None:intergenic
TCGTTGTGTGATAGATGCGC+CGG 0.591718 2_2:+59500538 Msa0261230:CDS
AAAGCCTCCGGTGCTAGGCT+CGG 0.592338 2_2:+59500505 Msa0261230:CDS
CCGGAGGCTTTCCACGGCGT+GGG 0.600004 2_2:-59500493 None:intergenic
GATGAAGAATGGGTAAGAAG+AGG 0.601495 2_2:-59500166 None:intergenic
CTAGCACCGGAGGCTTTCCA+CGG 0.603148 2_2:-59500499 None:intergenic
CCAGCATAAGCTAGCCTGGT+CGG 0.605872 2_2:-59500389 None:intergenic
CGAGGAACCGTCGTCAAAGG+CGG 0.613549 2_2:+59501055 Msa0261230:CDS
CGATTCCGATGAGGAAGATG+AGG 0.619273 2_2:+59500657 Msa0261230:CDS
CTACCGGAAGAACTCGAAGC+CGG 0.624941 2_2:-59500745 None:intergenic
GAGATTGACATGCGTGACAG+AGG 0.627483 2_2:-59500471 None:intergenic
CGTCGTCAAAGGCGGCGATG+CGG 0.629213 2_2:+59501063 Msa0261230:CDS
CATGTCAATCTCCCACGCCG+TGG 0.644127 2_2:+59500482 Msa0261230:CDS
GTTACCGAGCCTAGCACCGG+AGG 0.644615 2_2:-59500509 None:intergenic
ATAGTACGGCCTCAATCGTG+TGG 0.647407 2_2:-59501122 None:intergenic
CCGGAAGAACTCGAAGCCGG+TGG 0.652243 2_2:-59500742 None:intergenic
GTATAGCGATGTAAATGGAA+CGG 0.655447 2_2:+59501088 Msa0261230:CDS
TTGTGTGATAGATGCGCCGG+CGG 0.662123 2_2:+59500541 Msa0261230:CDS
GTGATAATCAGGTTGTGCCG+TGG 0.677277 2_2:+59500698 Msa0261230:CDS
GAGCAACAAGAAGAAAGTGA+AGG 0.680813 2_2:+59500610 Msa0261230:CDS
AGAAGACACCGATTCCGATG+AGG 0.691256 2_2:+59500648 Msa0261230:CDS
TGCGCCGCTGTTGATGACCA+CGG 0.697626 2_2:-59500715 None:intergenic
TGATTTGATAGGGTGCGACG+AGG 0.704245 2_2:+59501037 Msa0261230:CDS
TGTGCCGTGGTCATCAACAG+CGG 0.705571 2_2:+59500711 Msa0261230:CDS
GTGCAACGATAACCACGATG+AGG 0.723607 2_2:+59500792 Msa0261230:CDS
GCAAAGCTACTAAACCCACA+AGG 0.727371 2_2:-59501303 None:intergenic
ACTTAGAAATAACCTCATCG+TGG 0.742114 2_2:-59500804 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACAAAAATAATAATCATA+TGG - chr2_2:59501419-59501438 None:intergenic 10.0%
!! TTACAAAAATAATAATCATA+TGG - chr2_2:59501419-59501438 None:intergenic 10.0%
!! ATAATATCTCTAATATAGTA+CGG - chr2_2:59501139-59501158 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG + chr2_2:59501155-59501174 Msa0261230:three_prime_UTR 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG + chr2_2:59501267-59501286 Msa0261230:three_prime_UTR 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG + chr2_2:59501355-59501374 Msa0261230:three_prime_UTR 15.0%
!! ATAATATCTCTAATATAGTA+CGG - chr2_2:59501139-59501158 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG + chr2_2:59501155-59501174 Msa0261230:three_prime_UTR 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG + chr2_2:59501267-59501286 Msa0261230:three_prime_UTR 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG + chr2_2:59501355-59501374 Msa0261230:three_prime_UTR 15.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG - chr2_2:59500265-59500284 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG + chr2_2:59500965-59500984 Msa0261230:intron 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG + chr2_2:59501274-59501293 Msa0261230:three_prime_UTR 20.0%
!! TGTTTATATAACTTAGGTAA+AGG + chr2_2:59501361-59501380 Msa0261230:three_prime_UTR 20.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG - chr2_2:59500265-59500284 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG + chr2_2:59500965-59500984 Msa0261230:intron 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG + chr2_2:59501274-59501293 Msa0261230:three_prime_UTR 20.0%
!! TGTTTATATAACTTAGGTAA+AGG + chr2_2:59501361-59501380 Msa0261230:three_prime_UTR 20.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG + chr2_2:59500971-59500990 Msa0261230:intron 25.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG + chr2_2:59500971-59500990 Msa0261230:intron 25.0%
! TCGTTTAGATCATGATTTTC+AGG + chr2_2:59500855-59500874 Msa0261230:CDS 30.0%
AGGTTACGGATTCAAAAAAT+TGG + chr2_2:59500985-59501004 Msa0261230:intron 30.0%
!! TATTTTGAGGATTATGGTTG+TGG + chr2_2:59501280-59501299 Msa0261230:three_prime_UTR 30.0%
! TCGTTTAGATCATGATTTTC+AGG + chr2_2:59500855-59500874 Msa0261230:CDS 30.0%
AGGTTACGGATTCAAAAAAT+TGG + chr2_2:59500985-59501004 Msa0261230:intron 30.0%
!! TATTTTGAGGATTATGGTTG+TGG + chr2_2:59501280-59501299 Msa0261230:three_prime_UTR 30.0%
GTTTGGTATGGATGAAGAAT+GGG - chr2_2:59500179-59500198 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG - chr2_2:59500191-59500210 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG - chr2_2:59500196-59500215 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG - chr2_2:59500201-59500220 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG - chr2_2:59500334-59500353 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG - chr2_2:59500611-59500630 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG + chr2_2:59500687-59500706 Msa0261230:CDS 35.0%
ACTTAGAAATAACCTCATCG+TGG - chr2_2:59500807-59500826 None:intergenic 35.0%
TTGGAAAAATCAACGAAGAG+AGG + chr2_2:59501004-59501023 Msa0261230:CDS 35.0%
!! TGGATGAGGTTGATTTGATA+GGG + chr2_2:59501027-59501046 Msa0261230:CDS 35.0%
GTATAGCGATGTAAATGGAA+CGG + chr2_2:59501088-59501107 Msa0261230:CDS 35.0%
GTTTGGTATGGATGAAGAAT+GGG - chr2_2:59500179-59500198 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG - chr2_2:59500191-59500210 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG - chr2_2:59500196-59500215 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG - chr2_2:59500201-59500220 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG - chr2_2:59500334-59500353 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG - chr2_2:59500611-59500630 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG + chr2_2:59500687-59500706 Msa0261230:CDS 35.0%
ACTTAGAAATAACCTCATCG+TGG - chr2_2:59500807-59500826 None:intergenic 35.0%
TTGGAAAAATCAACGAAGAG+AGG + chr2_2:59501004-59501023 Msa0261230:CDS 35.0%
!! TGGATGAGGTTGATTTGATA+GGG + chr2_2:59501027-59501046 Msa0261230:CDS 35.0%
GTATAGCGATGTAAATGGAA+CGG + chr2_2:59501088-59501107 Msa0261230:CDS 35.0%
GATGAAGAATGGGTAAGAAG+AGG - chr2_2:59500169-59500188 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG - chr2_2:59500180-59500199 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG - chr2_2:59500303-59500322 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG + chr2_2:59500610-59500629 Msa0261230:CDS 40.0%
GAAAAATCAACGAAGAGAGG+TGG + chr2_2:59501007-59501026 Msa0261230:CDS 40.0%
!! GTGGATGAGGTTGATTTGAT+AGG + chr2_2:59501026-59501045 Msa0261230:CDS 40.0%
GATGAAGAATGGGTAAGAAG+AGG - chr2_2:59500169-59500188 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG - chr2_2:59500180-59500199 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG - chr2_2:59500303-59500322 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG + chr2_2:59500610-59500629 Msa0261230:CDS 40.0%
GAAAAATCAACGAAGAGAGG+TGG + chr2_2:59501007-59501026 Msa0261230:CDS 40.0%
!! GTGGATGAGGTTGATTTGAT+AGG + chr2_2:59501026-59501045 Msa0261230:CDS 40.0%
! GACTCACAGAAAGTTCTAGC+TGG - chr2_2:59500370-59500389 None:intergenic 45.0%
CAACCAAAAAGTTAGCAGCG+TGG - chr2_2:59500432-59500451 None:intergenic 45.0%
CTCATCTTCCTCATCGGAAT+CGG - chr2_2:59500659-59500678 None:intergenic 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG + chr2_2:59501289-59501308 Msa0261230:three_prime_UTR 45.0%
GCAAAGCTACTAAACCCACA+AGG - chr2_2:59501306-59501325 None:intergenic 45.0%
! GACTCACAGAAAGTTCTAGC+TGG - chr2_2:59500370-59500389 None:intergenic 45.0%
CAACCAAAAAGTTAGCAGCG+TGG - chr2_2:59500432-59500451 None:intergenic 45.0%
CTCATCTTCCTCATCGGAAT+CGG - chr2_2:59500659-59500678 None:intergenic 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG + chr2_2:59501289-59501308 Msa0261230:three_prime_UTR 45.0%
GCAAAGCTACTAAACCCACA+AGG - chr2_2:59501306-59501325 None:intergenic 45.0%
ACGAAACCGCGTTGAGAGAA+AGG + chr2_2:59500294-59500313 Msa0261230:five_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG + chr2_2:59500375-59500394 Msa0261230:CDS 50.0%
ATGCTGGGACTGCGATTCTA+AGG + chr2_2:59500405-59500424 Msa0261230:CDS 50.0%
! GGTCCACGCTGCTAACTTTT+TGG + chr2_2:59500426-59500445 Msa0261230:CDS 50.0%
GAGATTGACATGCGTGACAG+AGG - chr2_2:59500474-59500493 None:intergenic 50.0%
TCGTTGTGTGATAGATGCGC+CGG + chr2_2:59500538-59500557 Msa0261230:CDS 50.0%
!! AGAAGACACCGATTCCGATG+AGG + chr2_2:59500648-59500667 Msa0261230:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG + chr2_2:59500657-59500676 Msa0261230:CDS 50.0%
CTCGTCCTCATCTTCCTCAT+CGG - chr2_2:59500665-59500684 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG + chr2_2:59500676-59500695 Msa0261230:CDS 50.0%
! GTGATAATCAGGTTGTGCCG+TGG + chr2_2:59500698-59500717 Msa0261230:CDS 50.0%
TTCTTCCGGTAGCGATGAGT+CGG + chr2_2:59500756-59500775 Msa0261230:CDS 50.0%
GTGCAACGATAACCACGATG+AGG + chr2_2:59500792-59500811 Msa0261230:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG + chr2_2:59501013-59501032 Msa0261230:CDS 50.0%
TGATTTGATAGGGTGCGACG+AGG + chr2_2:59501037-59501056 Msa0261230:CDS 50.0%
CGGCGGTATAGCGATGTAAA+TGG + chr2_2:59501083-59501102 Msa0261230:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG + chr2_2:59501113-59501132 Msa0261230:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG - chr2_2:59501125-59501144 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG + chr2_2:59501288-59501307 Msa0261230:three_prime_UTR 50.0%
ACGAAACCGCGTTGAGAGAA+AGG + chr2_2:59500294-59500313 Msa0261230:five_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG + chr2_2:59500375-59500394 Msa0261230:CDS 50.0%
ATGCTGGGACTGCGATTCTA+AGG + chr2_2:59500405-59500424 Msa0261230:CDS 50.0%
! GGTCCACGCTGCTAACTTTT+TGG + chr2_2:59500426-59500445 Msa0261230:CDS 50.0%
GAGATTGACATGCGTGACAG+AGG - chr2_2:59500474-59500493 None:intergenic 50.0%
TCGTTGTGTGATAGATGCGC+CGG + chr2_2:59500538-59500557 Msa0261230:CDS 50.0%
!! AGAAGACACCGATTCCGATG+AGG + chr2_2:59500648-59500667 Msa0261230:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG + chr2_2:59500657-59500676 Msa0261230:CDS 50.0%
CTCGTCCTCATCTTCCTCAT+CGG - chr2_2:59500665-59500684 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG + chr2_2:59500676-59500695 Msa0261230:CDS 50.0%
! GTGATAATCAGGTTGTGCCG+TGG + chr2_2:59500698-59500717 Msa0261230:CDS 50.0%
TTCTTCCGGTAGCGATGAGT+CGG + chr2_2:59500756-59500775 Msa0261230:CDS 50.0%
GTGCAACGATAACCACGATG+AGG + chr2_2:59500792-59500811 Msa0261230:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG + chr2_2:59501013-59501032 Msa0261230:CDS 50.0%
TGATTTGATAGGGTGCGACG+AGG + chr2_2:59501037-59501056 Msa0261230:CDS 50.0%
CGGCGGTATAGCGATGTAAA+TGG + chr2_2:59501083-59501102 Msa0261230:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG + chr2_2:59501113-59501132 Msa0261230:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG - chr2_2:59501125-59501144 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG + chr2_2:59501288-59501307 Msa0261230:three_prime_UTR 50.0%
! CCAGCATAAGCTAGCCTGGT+CGG - chr2_2:59500392-59500411 None:intergenic 55.0%
!! CGACCAGGCTAGCTTATGCT+GGG + chr2_2:59500390-59500409 Msa0261230:CDS 55.0%
AGTCCCAGCATAAGCTAGCC+TGG - chr2_2:59500396-59500415 None:intergenic 55.0%
TTGTGTGATAGATGCGCCGG+CGG + chr2_2:59500541-59500560 Msa0261230:CDS 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG - chr2_2:59500605-59500624 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG + chr2_2:59500663-59500682 Msa0261230:CDS 55.0%
TGTGCCGTGGTCATCAACAG+CGG + chr2_2:59500711-59500730 Msa0261230:CDS 55.0%
CTACCGGAAGAACTCGAAGC+CGG - chr2_2:59500748-59500767 None:intergenic 55.0%
! CGTAACCGACTCATCGCTAC+CGG - chr2_2:59500764-59500783 None:intergenic 55.0%
CGGTAGCGATGAGTCGGTTA+CGG + chr2_2:59500762-59500781 Msa0261230:CDS 55.0%
! CCAGCATAAGCTAGCCTGGT+CGG - chr2_2:59500392-59500411 None:intergenic 55.0%
!! CGACCAGGCTAGCTTATGCT+GGG + chr2_2:59500390-59500409 Msa0261230:CDS 55.0%
AGTCCCAGCATAAGCTAGCC+TGG - chr2_2:59500396-59500415 None:intergenic 55.0%
TTGTGTGATAGATGCGCCGG+CGG + chr2_2:59500541-59500560 Msa0261230:CDS 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG - chr2_2:59500605-59500624 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG + chr2_2:59500663-59500682 Msa0261230:CDS 55.0%
TGTGCCGTGGTCATCAACAG+CGG + chr2_2:59500711-59500730 Msa0261230:CDS 55.0%
CTACCGGAAGAACTCGAAGC+CGG - chr2_2:59500748-59500767 None:intergenic 55.0%
! CGTAACCGACTCATCGCTAC+CGG - chr2_2:59500764-59500783 None:intergenic 55.0%
CGGTAGCGATGAGTCGGTTA+CGG + chr2_2:59500762-59500781 Msa0261230:CDS 55.0%
!! CCGACCAGGCTAGCTTATGC+TGG + chr2_2:59500389-59500408 Msa0261230:CDS 60.0%
CATGTCAATCTCCCACGCCG+TGG + chr2_2:59500482-59500501 Msa0261230:CDS 60.0%
! CTAGCACCGGAGGCTTTCCA+CGG - chr2_2:59500502-59500521 None:intergenic 60.0%
! AAAGCCTCCGGTGCTAGGCT+CGG + chr2_2:59500505-59500524 Msa0261230:CDS 60.0%
! GGCGTGGAGTTTTCTTCCGC+CGG - chr2_2:59500560-59500579 None:intergenic 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG - chr2_2:59500576-59500595 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG + chr2_2:59500577-59500596 Msa0261230:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG - chr2_2:59500581-59500600 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG - chr2_2:59500718-59500737 None:intergenic 60.0%
GAAGAACTCGAAGCCGGTGG+TGG - chr2_2:59500742-59500761 None:intergenic 60.0%
!! CCACCGGCTTCGAGTTCTTC+CGG + chr2_2:59500742-59500761 Msa0261230:CDS 60.0%
CGACGAGGAACCGTCGTCAA+AGG + chr2_2:59501052-59501071 Msa0261230:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG + chr2_2:59501055-59501074 Msa0261230:CDS 60.0%
!! CCGACCAGGCTAGCTTATGC+TGG + chr2_2:59500389-59500408 Msa0261230:CDS 60.0%
CATGTCAATCTCCCACGCCG+TGG + chr2_2:59500482-59500501 Msa0261230:CDS 60.0%
! CTAGCACCGGAGGCTTTCCA+CGG - chr2_2:59500502-59500521 None:intergenic 60.0%
! AAAGCCTCCGGTGCTAGGCT+CGG + chr2_2:59500505-59500524 Msa0261230:CDS 60.0%
! GGCGTGGAGTTTTCTTCCGC+CGG - chr2_2:59500560-59500579 None:intergenic 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG - chr2_2:59500576-59500595 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG + chr2_2:59500577-59500596 Msa0261230:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG - chr2_2:59500581-59500600 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG - chr2_2:59500718-59500737 None:intergenic 60.0%
GAAGAACTCGAAGCCGGTGG+TGG - chr2_2:59500742-59500761 None:intergenic 60.0%
!! CCACCGGCTTCGAGTTCTTC+CGG + chr2_2:59500742-59500761 Msa0261230:CDS 60.0%
CGACGAGGAACCGTCGTCAA+AGG + chr2_2:59501052-59501071 Msa0261230:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG + chr2_2:59501055-59501074 Msa0261230:CDS 60.0%
CGTGGAAAGCCTCCGGTGCT+AGG + chr2_2:59500500-59500519 Msa0261230:CDS 65.0%
! GTTACCGAGCCTAGCACCGG+AGG - chr2_2:59500512-59500531 None:intergenic 65.0%
CGCGTTACCGAGCCTAGCAC+CGG - chr2_2:59500515-59500534 None:intergenic 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG - chr2_2:59500599-59500618 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG + chr2_2:59500726-59500745 Msa0261230:CDS 65.0%
CCGGAAGAACTCGAAGCCGG+TGG - chr2_2:59500745-59500764 None:intergenic 65.0%
CGCATCGCCGCCTTTGACGA+CGG - chr2_2:59501065-59501084 None:intergenic 65.0%
CGTCGTCAAAGGCGGCGATG+CGG + chr2_2:59501063-59501082 Msa0261230:CDS 65.0%
CGTGGAAAGCCTCCGGTGCT+AGG + chr2_2:59500500-59500519 Msa0261230:CDS 65.0%
! GTTACCGAGCCTAGCACCGG+AGG - chr2_2:59500512-59500531 None:intergenic 65.0%
CGCGTTACCGAGCCTAGCAC+CGG - chr2_2:59500515-59500534 None:intergenic 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG - chr2_2:59500599-59500618 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG + chr2_2:59500726-59500745 Msa0261230:CDS 65.0%
CCGGAAGAACTCGAAGCCGG+TGG - chr2_2:59500745-59500764 None:intergenic 65.0%
CGCATCGCCGCCTTTGACGA+CGG - chr2_2:59501065-59501084 None:intergenic 65.0%
CGTCGTCAAAGGCGGCGATG+CGG + chr2_2:59501063-59501082 Msa0261230:CDS 65.0%
CCGGAGGCTTTCCACGGCGT+GGG - chr2_2:59500496-59500515 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG + chr2_2:59500493-59500512 Msa0261230:CDS 70.0%
ACCGGAGGCTTTCCACGGCG+TGG - chr2_2:59500497-59500516 None:intergenic 70.0%
CGTCAAAGGCGGCGATGCGG+CGG + chr2_2:59501066-59501085 Msa0261230:CDS 70.0%
CCGGAGGCTTTCCACGGCGT+GGG - chr2_2:59500496-59500515 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG + chr2_2:59500493-59500512 Msa0261230:CDS 70.0%
ACCGGAGGCTTTCCACGGCG+TGG - chr2_2:59500497-59500516 None:intergenic 70.0%
CGTCAAAGGCGGCGATGCGG+CGG + chr2_2:59501066-59501085 Msa0261230:CDS 70.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG - chr2_2:59500593-59500612 None:intergenic 80.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG - chr2_2:59500593-59500612 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 59500163 59501474 59500163 ID=Msa0261230;Name=Msa0261230
chr2_2 mRNA 59500163 59501474 59500163 ID=Msa0261230-mRNA-1;Parent=Msa0261230;Name=Msa0261230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=174|1|1|1|1|1|2|341|228
chr2_2 exon 59500163 59500876 59500163 ID=Msa0261230-mRNA-1:exon:18337;Parent=Msa0261230-mRNA-1
chr2_2 exon 59500987 59501474 59500987 ID=Msa0261230-mRNA-1:exon:18338;Parent=Msa0261230-mRNA-1
chr2_2 five_prime_UTR 59500163 59500336 59500163 ID=Msa0261230-mRNA-1:five_prime_utr;Parent=Msa0261230-mRNA-1
chr2_2 CDS 59500337 59500876 59500337 ID=Msa0261230-mRNA-1:cds;Parent=Msa0261230-mRNA-1
chr2_2 CDS 59500987 59501133 59500987 ID=Msa0261230-mRNA-1:cds;Parent=Msa0261230-mRNA-1
chr2_2 three_prime_UTR 59501134 59501474 59501134 ID=Msa0261230-mRNA-1:three_prime_utr;Parent=Msa0261230-mRNA-1
Gene Sequence

>Msa0261230

ATGAAAAAGAACTGCGAACTCTGCAAGCTTCCAGCTAGAACTTTCTGTGAGTCCGACCAGGCTAGCTTATGCTGGGACTGCGATTCTAAGGTCCACGCTGCTAACTTTTTGGTTGAGAGACACATGAGAACTCTCCTCTGTCACGCATGTCAATCTCCCACGCCGTGGAAAGCCTCCGGTGCTAGGCTCGGTAACGCGCTTTCGTTGTGTGATAGATGCGCCGGCGGAAGAAAACTCCACGCCAACGCTAACTCCAACGCCGGTGCCAGCGCCGAGCAACAAGAAGAAAGTGAAGGTGACAATGACAATGAAGAAGACACCGATTCCGATGAGGAAGATGAGGACGAGGATGAAGAAGATGGTGATAATCAGGTTGTGCCGTGGTCATCAACAGCGGCGCAACCACCACCGGCTTCGAGTTCTTCCGGTAGCGATGAGTCGGTTACGGTTAGCAAGTGCAACGATAACCACGATGAGGTTATTTCTAAGTTAGTTACAACGATTTCTTTGAAACGACGTCGTTTAGATCATGATTTTCAGGTTACGGATTCAAAAAATTGGAAAAATCAACGAAGAGAGGTGGATGAGGTTGATTTGATAGGGTGCGACGAGGAACCGTCGTCAAAGGCGGCGATGCGGCGGTATAGCGATGTAAATGGAACGGAGCATTCTCAGCCACACGATTGA
Protein sequence

>Msa0261230

MKKNCELCKLPARTFCESDQASLCWDCDSKVHAANFLVERHMRTLLCHACQSPTPWKASGARLGNALSLCDRCAGGRKLHANANSNAGASAEQQEESEGDNDNEEDTDSDEEDEDEDEEDGDNQVVPWSSTAAQPPPASSSSGSDESVTVSKCNDNHDEVISKLVTTISLKRRRLDHDFQVTDSKNWKNQRREVDEVDLIGCDEEPSSKAAMRRYSDVNGTEHSQPHD*