AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0299220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299220 MtrunA17_Chr2g0323271 90.558 233 19 2 1 230 1 233 1.73e-121 344
Msa0299220 MtrunA17_Chr2g0280901 47.586 145 49 2 1 145 1 118 1.83e-31 112
Msa0299220 MtrunA17_Chr2g0322971 52.542 59 28 0 3 61 29 87 1.62e-13 65.1
Msa0299220 MtrunA17_Chr4g0024021 50.847 59 29 0 5 63 36 94 1.03e-12 63.2
Msa0299220 MtrunA17_Chr4g0002211 50.000 66 33 0 3 68 6 71 2.32e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299220 AT4G27310.1 42.197 173 56 3 1 138 1 164 9.45e-36 126
Msa0299220 AT5G54470.1 47.059 187 78 4 2 188 3 168 5.11e-34 121
Msa0299220 AT3G21890.1 56.000 50 22 0 5 54 31 80 8.94e-11 58.2

Find 73 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGATGAGGTTGGTTTGAT+AGG 0.262299 2_3:-53817819 Msa0299220:CDS
CGGCGGTATAGCGACGTAAA+TGG 0.305299 2_3:-53817762 Msa0299220:CDS
TTCACTTTCTTCTTGTTGCT+CGG 0.346480 2_3:+53818243 None:intergenic
TTCTTCTTGTTGCTCGGCGC+TGG 0.356031 2_3:+53818249 None:intergenic
TTACAAAATTAATAATCATA+TGG 0.382752 2_3:+53817428 None:intergenic
ACGAAACCGCGTTGAGAGAA+AGG 0.398885 2_3:-53818557 Msa0299220:five_prime_UTR
ATGTTGGGACTGCGATTCTA+AGG 0.399501 2_3:-53818446 Msa0299220:CDS
TGAAGAAGATGGTGATAATC+AGG 0.403542 2_3:-53818158 Msa0299220:CDS
CGCATCGCCGCCTTTGACGA+CGG 0.405274 2_3:+53817783 None:intergenic
TTCCTCATCGGAATCGTAAT+CGG 0.408569 2_3:+53818195 None:intergenic
GATTATGGTTGTGGCCTTGT+GGG 0.422309 2_3:-53817555 Msa0299220:three_prime_UTR
TGGATGAGGTTGGTTTGATA+GGG 0.423166 2_3:-53817818 Msa0299220:CDS
CTCGGCGCTGGCACCGGCGT+TGG 0.429781 2_3:+53818261 None:intergenic
CCACCGGCTTCGAGTTCTTC+CGG 0.435713 2_3:-53818103 Msa0299220:CDS
TTTCTTGTGATGGTTTGGTA+TGG 0.436961 2_3:+53818639 None:intergenic
CGTGGAAAGCCTCCGGTGCT+AGG 0.445561 2_3:-53818351 Msa0299220:CDS
TTGTTGCTCGGCGCTGGCAC+CGG 0.453269 2_3:+53818255 None:intergenic
TGTTTATATAACTTAGGTAA+AGG 0.465810 2_3:-53817483 Msa0299220:three_prime_UTR
CGGTAGCGATGAGTCGGTTA+CGG 0.474621 2_3:-53818083 Msa0299220:CDS
GGTTTGGTATGGATGAAGAA+TGG 0.486262 2_3:+53818650 None:intergenic
GTTTGGTATGGATGAAGAAT+GGG 0.493335 2_3:+53818651 None:intergenic
CGACCAGGCTAGCTTATGTT+GGG 0.515990 2_3:-53818461 Msa0299220:CDS
CGCGTTACCGAGCCTAGCAC+CGG 0.516421 2_3:+53818339 None:intergenic
CGAAGAGAGGTGGATGAGGT+TGG 0.516662 2_3:-53817828 Msa0299220:CDS
GCCAACGCTAACTCCAACGC+CGG 0.517678 2_3:-53818274 Msa0299220:CDS
ATAATATCTCTAATATAGTA+CGG 0.521383 2_3:+53817709 None:intergenic
CTCGTCCTCATCTTCCTCAT+CGG 0.523137 2_3:+53818183 None:intergenic
GACTCACAGAAAGTTCTAGC+TGG 0.526494 2_3:+53818484 None:intergenic
ACCGGCGTTGGAGTTAGCGT+TGG 0.527253 2_3:+53818273 None:intergenic
AGTCCCAACATAAGCTAGCC+TGG 0.529010 2_3:+53818458 None:intergenic
CCGACCAGGCTAGCTTATGT+TGG 0.529135 2_3:-53818462 Msa0299220:CDS
GAGGACGAGGATGAAGAAGA+TGG 0.529527 2_3:-53818169 Msa0299220:CDS
AACTTTCTGTGAGTCCGACC+AGG 0.531925 2_3:-53818476 Msa0299220:CDS
ACCGGAGGCTTTCCACGGCG+TGG 0.539884 2_3:+53818357 None:intergenic
CCCACGCCGTGGAAAGCCTC+CGG 0.541657 2_3:-53818358 Msa0299220:CDS
CGATGAGGAAGATGAGGACG+AGG 0.544127 2_3:-53818182 Msa0299220:CDS
CGACGAGGAACCGTCGTCAA+AGG 0.547277 2_3:-53817793 Msa0299220:CDS
GAAGAACTCGAAGCCGGTGG+TGG 0.549423 2_3:+53818106 None:intergenic
CGTCAAAGGCGGCGATGCGG+CGG 0.550222 2_3:-53817779 Msa0299220:CDS
AACAGCGGCGCAACCACCAC+CGG 0.553693 2_3:-53818119 Msa0299220:CDS
GGATTATGGTTGTGGCCTTG+TGG 0.566677 2_3:-53817556 Msa0299220:three_prime_UTR
CATTCTCAGCCACACGATTG+AGG 0.570452 2_3:-53817732 Msa0299220:exon
CGTAACCGACTCATCGCTAC+CGG 0.571656 2_3:+53818084 None:intergenic
TCAACGAAGAGAGGTGGATG+AGG 0.575020 2_3:-53817832 Msa0299220:CDS
AGAATGGGTAAGAAGAGGAA+AGG 0.576671 2_3:+53818666 None:intergenic
TTCTTCCGGTAGCGATGAGT+CGG 0.577433 2_3:-53818089 Msa0299220:CDS
CGTTGGAGTTAGCGTTGGCG+TGG 0.586469 2_3:+53818278 None:intergenic
TCGTTGTGTGATAGATGCGC+CGG 0.591718 2_3:-53818313 Msa0299220:CDS
AAAGCCTCCGGTGCTAGGCT+CGG 0.592338 2_3:-53818346 Msa0299220:CDS
CCGGAGGCTTTCCACGGCGT+GGG 0.600004 2_3:+53818358 None:intergenic
GATGAAGAATGGGTAAGAAG+AGG 0.601495 2_3:+53818661 None:intergenic
CTAGCACCGGAGGCTTTCCA+CGG 0.603148 2_3:+53818352 None:intergenic
CGAGGAACCGTCGTCAAAGG+CGG 0.613549 2_3:-53817790 Msa0299220:CDS
CGATTCCGATGAGGAAGATG+AGG 0.616964 2_3:-53818188 Msa0299220:CDS
CTACCGGAAGAACTCGAAGC+CGG 0.624941 2_3:+53818100 None:intergenic
GAGATTGACATGCGTGACAG+AGG 0.627483 2_3:+53818380 None:intergenic
CCAACATAAGCTAGCCTGGT+CGG 0.628810 2_3:+53818462 None:intergenic
CGTCGTCAAAGGCGGCGATG+CGG 0.629213 2_3:-53817782 Msa0299220:CDS
GTATAGCGACGTAAATGGAA+CGG 0.632422 2_3:-53817757 Msa0299220:CDS
CATGTCAATCTCCCACGCCG+TGG 0.644127 2_3:-53818369 Msa0299220:CDS
GTTACCGAGCCTAGCACCGG+AGG 0.644615 2_3:+53818342 None:intergenic
ATAGTACGGCCTCAATCGTG+TGG 0.647407 2_3:+53817723 None:intergenic
CCGGAAGAACTCGAAGCCGG+TGG 0.652243 2_3:+53818103 None:intergenic
TTGTGTGATAGATGCGCCGG+CGG 0.662123 2_3:-53818310 Msa0299220:CDS
GTGATAATCAGGTTGTGCCG+TGG 0.677277 2_3:-53818147 Msa0299220:CDS
GAGCAACAAGAAGAAAGTGA+AGG 0.680813 2_3:-53818241 Msa0299220:CDS
CACCGATTACGATTCCGATG+AGG 0.696165 2_3:-53818197 Msa0299220:CDS
TGCGCCGCTGTTGATGACCA+CGG 0.697626 2_3:+53818130 None:intergenic
TGGTTTGATAGGGTGCGACG+AGG 0.704245 2_3:-53817808 Msa0299220:CDS
TGTGCCGTGGTCATCAACAG+CGG 0.705571 2_3:-53818134 Msa0299220:CDS
GTGCAACGATAACCACGATG+AGG 0.723607 2_3:-53818053 Msa0299220:CDS
GCAAAGCTACTAAACCCACA+AGG 0.727371 2_3:+53817541 None:intergenic
ACTTAGAAATAACCTCATCG+TGG 0.742114 2_3:+53818041 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACAAAATTAATAATCATA+TGG + chr2_3:53818631-53818650 None:intergenic 10.0%
!! TTACAAAATTAATAATCATA+TGG + chr2_3:53818631-53818650 None:intergenic 10.0%
!! ATAATATCTCTAATATAGTA+CGG + chr2_3:53818350-53818369 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG - chr2_3:53818366-53818385 Msa0299220:CDS 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG - chr2_3:53818479-53818498 Msa0299220:CDS 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG - chr2_3:53818567-53818586 Msa0299220:five_prime_UTR 15.0%
!! ATAATATCTCTAATATAGTA+CGG + chr2_3:53818350-53818369 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG - chr2_3:53818366-53818385 Msa0299220:CDS 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG - chr2_3:53818479-53818498 Msa0299220:CDS 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG - chr2_3:53818567-53818586 Msa0299220:five_prime_UTR 15.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG + chr2_3:53817470-53817489 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG - chr2_3:53818176-53818195 Msa0299220:CDS 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG - chr2_3:53818486-53818505 Msa0299220:CDS 20.0%
!! TGTTTATATAACTTAGGTAA+AGG - chr2_3:53818573-53818592 Msa0299220:five_prime_UTR 20.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG + chr2_3:53817470-53817489 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG - chr2_3:53818176-53818195 Msa0299220:CDS 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG - chr2_3:53818486-53818505 Msa0299220:CDS 20.0%
!! TGTTTATATAACTTAGGTAA+AGG - chr2_3:53818573-53818592 Msa0299220:five_prime_UTR 20.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG - chr2_3:53818182-53818201 Msa0299220:CDS 25.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG - chr2_3:53818182-53818201 Msa0299220:CDS 25.0%
! TCGTTTAGATCATGATTTTC+AGG - chr2_3:53818066-53818085 Msa0299220:CDS 30.0%
AGGTTACGGATTCAAAAAAT+TGG - chr2_3:53818196-53818215 Msa0299220:CDS 30.0%
!! TATTTTGAGGATTATGGTTG+TGG - chr2_3:53818492-53818511 Msa0299220:CDS 30.0%
! TCGTTTAGATCATGATTTTC+AGG - chr2_3:53818066-53818085 Msa0299220:CDS 30.0%
AGGTTACGGATTCAAAAAAT+TGG - chr2_3:53818196-53818215 Msa0299220:CDS 30.0%
!! TATTTTGAGGATTATGGTTG+TGG - chr2_3:53818492-53818511 Msa0299220:CDS 30.0%
GTTTGGTATGGATGAAGAAT+GGG + chr2_3:53817408-53817427 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG + chr2_3:53817420-53817439 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG + chr2_3:53817425-53817444 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG + chr2_3:53817430-53817449 None:intergenic 35.0%
! TTTGCGGAATTTTCGTGTTT+GGG + chr2_3:53817454-53817473 None:intergenic 35.0%
! TTTTGCGGAATTTTCGTGTT+TGG + chr2_3:53817455-53817474 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG + chr2_3:53817539-53817558 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG + chr2_3:53817816-53817835 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG - chr2_3:53817898-53817917 Msa0299220:intron 35.0%
ACTTAGAAATAACCTCATCG+TGG + chr2_3:53818018-53818037 None:intergenic 35.0%
TTGGAAAAATCAACGAAGAG+AGG - chr2_3:53818215-53818234 Msa0299220:CDS 35.0%
GTTTGGTATGGATGAAGAAT+GGG + chr2_3:53817408-53817427 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG + chr2_3:53817420-53817439 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG + chr2_3:53817425-53817444 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG + chr2_3:53817430-53817449 None:intergenic 35.0%
! TTTGCGGAATTTTCGTGTTT+GGG + chr2_3:53817454-53817473 None:intergenic 35.0%
! TTTTGCGGAATTTTCGTGTT+TGG + chr2_3:53817455-53817474 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG + chr2_3:53817539-53817558 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG + chr2_3:53817816-53817835 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG - chr2_3:53817898-53817917 Msa0299220:intron 35.0%
ACTTAGAAATAACCTCATCG+TGG + chr2_3:53818018-53818037 None:intergenic 35.0%
TTGGAAAAATCAACGAAGAG+AGG - chr2_3:53818215-53818234 Msa0299220:CDS 35.0%
GATGAAGAATGGGTAAGAAG+AGG + chr2_3:53817398-53817417 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG + chr2_3:53817409-53817428 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG + chr2_3:53817508-53817527 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG - chr2_3:53817815-53817834 Msa0299220:CDS 40.0%
TTCCTCATCGGAATCGTAAT+CGG + chr2_3:53817864-53817883 None:intergenic 40.0%
GAAAAATCAACGAAGAGAGG+TGG - chr2_3:53818218-53818237 Msa0299220:CDS 40.0%
!! TGGATGAGGTTGGTTTGATA+GGG - chr2_3:53818238-53818257 Msa0299220:CDS 40.0%
GTATAGCGACGTAAATGGAA+CGG - chr2_3:53818299-53818318 Msa0299220:CDS 40.0%
GATGAAGAATGGGTAAGAAG+AGG + chr2_3:53817398-53817417 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG + chr2_3:53817409-53817428 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG + chr2_3:53817508-53817527 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG - chr2_3:53817815-53817834 Msa0299220:CDS 40.0%
TTCCTCATCGGAATCGTAAT+CGG + chr2_3:53817864-53817883 None:intergenic 40.0%
GAAAAATCAACGAAGAGAGG+TGG - chr2_3:53818218-53818237 Msa0299220:CDS 40.0%
!! TGGATGAGGTTGGTTTGATA+GGG - chr2_3:53818238-53818257 Msa0299220:CDS 40.0%
GTATAGCGACGTAAATGGAA+CGG - chr2_3:53818299-53818318 Msa0299220:CDS 40.0%
! GACTCACAGAAAGTTCTAGC+TGG + chr2_3:53817575-53817594 None:intergenic 45.0%
ATGTTGGGACTGCGATTCTA+AGG - chr2_3:53817610-53817629 Msa0299220:three_prime_UTR 45.0%
CAACCAAAAAGTTAGCAGCG+TGG + chr2_3:53817637-53817656 None:intergenic 45.0%
!! GTGGATGAGGTTGGTTTGAT+AGG - chr2_3:53818237-53818256 Msa0299220:CDS 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG - chr2_3:53818501-53818520 Msa0299220:CDS 45.0%
GCAAAGCTACTAAACCCACA+AGG + chr2_3:53818518-53818537 None:intergenic 45.0%
! GACTCACAGAAAGTTCTAGC+TGG + chr2_3:53817575-53817594 None:intergenic 45.0%
ATGTTGGGACTGCGATTCTA+AGG - chr2_3:53817610-53817629 Msa0299220:three_prime_UTR 45.0%
CAACCAAAAAGTTAGCAGCG+TGG + chr2_3:53817637-53817656 None:intergenic 45.0%
!! GTGGATGAGGTTGGTTTGAT+AGG - chr2_3:53818237-53818256 Msa0299220:CDS 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG - chr2_3:53818501-53818520 Msa0299220:CDS 45.0%
GCAAAGCTACTAAACCCACA+AGG + chr2_3:53818518-53818537 None:intergenic 45.0%
ACGAAACCGCGTTGAGAGAA+AGG - chr2_3:53817499-53817518 Msa0299220:three_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG - chr2_3:53817580-53817599 Msa0299220:three_prime_UTR 50.0%
! CCAACATAAGCTAGCCTGGT+CGG + chr2_3:53817597-53817616 None:intergenic 50.0%
!! CGACCAGGCTAGCTTATGTT+GGG - chr2_3:53817595-53817614 Msa0299220:three_prime_UTR 50.0%
AGTCCCAACATAAGCTAGCC+TGG + chr2_3:53817601-53817620 None:intergenic 50.0%
! GGTCCACGCTGCTAACTTTT+TGG - chr2_3:53817631-53817650 Msa0299220:three_prime_UTR 50.0%
GAGATTGACATGCGTGACAG+AGG + chr2_3:53817679-53817698 None:intergenic 50.0%
TCGTTGTGTGATAGATGCGC+CGG - chr2_3:53817743-53817762 Msa0299220:CDS 50.0%
! CACCGATTACGATTCCGATG+AGG - chr2_3:53817859-53817878 Msa0299220:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG - chr2_3:53817868-53817887 Msa0299220:intron 50.0%
CTCGTCCTCATCTTCCTCAT+CGG + chr2_3:53817876-53817895 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG - chr2_3:53817887-53817906 Msa0299220:intron 50.0%
! GTGATAATCAGGTTGTGCCG+TGG - chr2_3:53817909-53817928 Msa0299220:intron 50.0%
TTCTTCCGGTAGCGATGAGT+CGG - chr2_3:53817967-53817986 Msa0299220:intron 50.0%
GTGCAACGATAACCACGATG+AGG - chr2_3:53818003-53818022 Msa0299220:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG - chr2_3:53818224-53818243 Msa0299220:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG - chr2_3:53818324-53818343 Msa0299220:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG + chr2_3:53818336-53818355 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG - chr2_3:53818500-53818519 Msa0299220:CDS 50.0%
ACGAAACCGCGTTGAGAGAA+AGG - chr2_3:53817499-53817518 Msa0299220:three_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG - chr2_3:53817580-53817599 Msa0299220:three_prime_UTR 50.0%
! CCAACATAAGCTAGCCTGGT+CGG + chr2_3:53817597-53817616 None:intergenic 50.0%
!! CGACCAGGCTAGCTTATGTT+GGG - chr2_3:53817595-53817614 Msa0299220:three_prime_UTR 50.0%
AGTCCCAACATAAGCTAGCC+TGG + chr2_3:53817601-53817620 None:intergenic 50.0%
! GGTCCACGCTGCTAACTTTT+TGG - chr2_3:53817631-53817650 Msa0299220:three_prime_UTR 50.0%
GAGATTGACATGCGTGACAG+AGG + chr2_3:53817679-53817698 None:intergenic 50.0%
TCGTTGTGTGATAGATGCGC+CGG - chr2_3:53817743-53817762 Msa0299220:CDS 50.0%
! CACCGATTACGATTCCGATG+AGG - chr2_3:53817859-53817878 Msa0299220:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG - chr2_3:53817868-53817887 Msa0299220:intron 50.0%
CTCGTCCTCATCTTCCTCAT+CGG + chr2_3:53817876-53817895 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG - chr2_3:53817887-53817906 Msa0299220:intron 50.0%
! GTGATAATCAGGTTGTGCCG+TGG - chr2_3:53817909-53817928 Msa0299220:intron 50.0%
TTCTTCCGGTAGCGATGAGT+CGG - chr2_3:53817967-53817986 Msa0299220:intron 50.0%
GTGCAACGATAACCACGATG+AGG - chr2_3:53818003-53818022 Msa0299220:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG - chr2_3:53818224-53818243 Msa0299220:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG - chr2_3:53818324-53818343 Msa0299220:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG + chr2_3:53818336-53818355 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG - chr2_3:53818500-53818519 Msa0299220:CDS 50.0%
!! CCGACCAGGCTAGCTTATGT+TGG - chr2_3:53817594-53817613 Msa0299220:three_prime_UTR 55.0%
TTGTGTGATAGATGCGCCGG+CGG - chr2_3:53817746-53817765 Msa0299220:CDS 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG + chr2_3:53817810-53817829 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG - chr2_3:53817874-53817893 Msa0299220:intron 55.0%
TGTGCCGTGGTCATCAACAG+CGG - chr2_3:53817922-53817941 Msa0299220:intron 55.0%
CTACCGGAAGAACTCGAAGC+CGG + chr2_3:53817959-53817978 None:intergenic 55.0%
! CGTAACCGACTCATCGCTAC+CGG + chr2_3:53817975-53817994 None:intergenic 55.0%
CGGTAGCGATGAGTCGGTTA+CGG - chr2_3:53817973-53817992 Msa0299220:intron 55.0%
CGAAGAGAGGTGGATGAGGT+TGG - chr2_3:53818228-53818247 Msa0299220:CDS 55.0%
TGGTTTGATAGGGTGCGACG+AGG - chr2_3:53818248-53818267 Msa0299220:CDS 55.0%
CGGCGGTATAGCGACGTAAA+TGG - chr2_3:53818294-53818313 Msa0299220:CDS 55.0%
!! CCGACCAGGCTAGCTTATGT+TGG - chr2_3:53817594-53817613 Msa0299220:three_prime_UTR 55.0%
TTGTGTGATAGATGCGCCGG+CGG - chr2_3:53817746-53817765 Msa0299220:CDS 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG + chr2_3:53817810-53817829 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG - chr2_3:53817874-53817893 Msa0299220:intron 55.0%
TGTGCCGTGGTCATCAACAG+CGG - chr2_3:53817922-53817941 Msa0299220:intron 55.0%
CTACCGGAAGAACTCGAAGC+CGG + chr2_3:53817959-53817978 None:intergenic 55.0%
! CGTAACCGACTCATCGCTAC+CGG + chr2_3:53817975-53817994 None:intergenic 55.0%
CGGTAGCGATGAGTCGGTTA+CGG - chr2_3:53817973-53817992 Msa0299220:intron 55.0%
CGAAGAGAGGTGGATGAGGT+TGG - chr2_3:53818228-53818247 Msa0299220:CDS 55.0%
TGGTTTGATAGGGTGCGACG+AGG - chr2_3:53818248-53818267 Msa0299220:CDS 55.0%
CGGCGGTATAGCGACGTAAA+TGG - chr2_3:53818294-53818313 Msa0299220:CDS 55.0%
CATGTCAATCTCCCACGCCG+TGG - chr2_3:53817687-53817706 Msa0299220:three_prime_UTR 60.0%
! CTAGCACCGGAGGCTTTCCA+CGG + chr2_3:53817707-53817726 None:intergenic 60.0%
! AAAGCCTCCGGTGCTAGGCT+CGG - chr2_3:53817710-53817729 Msa0299220:three_prime_UTR 60.0%
! GGCGTGGAGTTTTCTTCCGC+CGG + chr2_3:53817765-53817784 None:intergenic 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG + chr2_3:53817781-53817800 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG - chr2_3:53817782-53817801 Msa0299220:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG + chr2_3:53817786-53817805 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG + chr2_3:53817929-53817948 None:intergenic 60.0%
GAAGAACTCGAAGCCGGTGG+TGG + chr2_3:53817953-53817972 None:intergenic 60.0%
!! CCACCGGCTTCGAGTTCTTC+CGG - chr2_3:53817953-53817972 Msa0299220:intron 60.0%
CGACGAGGAACCGTCGTCAA+AGG - chr2_3:53818263-53818282 Msa0299220:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG - chr2_3:53818266-53818285 Msa0299220:CDS 60.0%
CATGTCAATCTCCCACGCCG+TGG - chr2_3:53817687-53817706 Msa0299220:three_prime_UTR 60.0%
! CTAGCACCGGAGGCTTTCCA+CGG + chr2_3:53817707-53817726 None:intergenic 60.0%
! AAAGCCTCCGGTGCTAGGCT+CGG - chr2_3:53817710-53817729 Msa0299220:three_prime_UTR 60.0%
! GGCGTGGAGTTTTCTTCCGC+CGG + chr2_3:53817765-53817784 None:intergenic 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG + chr2_3:53817781-53817800 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG - chr2_3:53817782-53817801 Msa0299220:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG + chr2_3:53817786-53817805 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG + chr2_3:53817929-53817948 None:intergenic 60.0%
GAAGAACTCGAAGCCGGTGG+TGG + chr2_3:53817953-53817972 None:intergenic 60.0%
!! CCACCGGCTTCGAGTTCTTC+CGG - chr2_3:53817953-53817972 Msa0299220:intron 60.0%
CGACGAGGAACCGTCGTCAA+AGG - chr2_3:53818263-53818282 Msa0299220:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG - chr2_3:53818266-53818285 Msa0299220:CDS 60.0%
CGTGGAAAGCCTCCGGTGCT+AGG - chr2_3:53817705-53817724 Msa0299220:three_prime_UTR 65.0%
! GTTACCGAGCCTAGCACCGG+AGG + chr2_3:53817717-53817736 None:intergenic 65.0%
CGCGTTACCGAGCCTAGCAC+CGG + chr2_3:53817720-53817739 None:intergenic 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG + chr2_3:53817804-53817823 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG - chr2_3:53817937-53817956 Msa0299220:intron 65.0%
CCGGAAGAACTCGAAGCCGG+TGG + chr2_3:53817956-53817975 None:intergenic 65.0%
CGCATCGCCGCCTTTGACGA+CGG + chr2_3:53818276-53818295 None:intergenic 65.0%
CGTCGTCAAAGGCGGCGATG+CGG - chr2_3:53818274-53818293 Msa0299220:CDS 65.0%
CGTGGAAAGCCTCCGGTGCT+AGG - chr2_3:53817705-53817724 Msa0299220:three_prime_UTR 65.0%
! GTTACCGAGCCTAGCACCGG+AGG + chr2_3:53817717-53817736 None:intergenic 65.0%
CGCGTTACCGAGCCTAGCAC+CGG + chr2_3:53817720-53817739 None:intergenic 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG + chr2_3:53817804-53817823 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG - chr2_3:53817937-53817956 Msa0299220:intron 65.0%
CCGGAAGAACTCGAAGCCGG+TGG + chr2_3:53817956-53817975 None:intergenic 65.0%
CGCATCGCCGCCTTTGACGA+CGG + chr2_3:53818276-53818295 None:intergenic 65.0%
CGTCGTCAAAGGCGGCGATG+CGG - chr2_3:53818274-53818293 Msa0299220:CDS 65.0%
CCGGAGGCTTTCCACGGCGT+GGG + chr2_3:53817701-53817720 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG - chr2_3:53817698-53817717 Msa0299220:three_prime_UTR 70.0%
ACCGGAGGCTTTCCACGGCG+TGG + chr2_3:53817702-53817721 None:intergenic 70.0%
CGTCAAAGGCGGCGATGCGG+CGG - chr2_3:53818277-53818296 Msa0299220:CDS 70.0%
CCGGAGGCTTTCCACGGCGT+GGG + chr2_3:53817701-53817720 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG - chr2_3:53817698-53817717 Msa0299220:three_prime_UTR 70.0%
ACCGGAGGCTTTCCACGGCG+TGG + chr2_3:53817702-53817721 None:intergenic 70.0%
CGTCAAAGGCGGCGATGCGG+CGG - chr2_3:53818277-53818296 Msa0299220:CDS 70.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG + chr2_3:53817798-53817817 None:intergenic 80.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG + chr2_3:53817798-53817817 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 53817392 53818686 53817392 ID=Msa0299220;Name=Msa0299220
chr2_3 mRNA 53817392 53818686 53817392 ID=Msa0299220-mRNA-1;Parent=Msa0299220;Name=Msa0299220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=150|1|1|1|1|1|2|342|230
chr2_3 exon 53817392 53817880 53817392 ID=Msa0299220-mRNA-1:exon:17219;Parent=Msa0299220-mRNA-1
chr2_3 exon 53817991 53818686 53817991 ID=Msa0299220-mRNA-1:exon:17218;Parent=Msa0299220-mRNA-1
chr2_3 five_prime_UTR 53818537 53818686 53818537 ID=Msa0299220-mRNA-1:five_prime_utr;Parent=Msa0299220-mRNA-1
chr2_3 CDS 53817991 53818536 53817991 ID=Msa0299220-mRNA-1:cds;Parent=Msa0299220-mRNA-1
chr2_3 CDS 53817734 53817880 53817734 ID=Msa0299220-mRNA-1:cds;Parent=Msa0299220-mRNA-1
chr2_3 three_prime_UTR 53817392 53817733 53817392 ID=Msa0299220-mRNA-1:three_prime_utr;Parent=Msa0299220-mRNA-1
Gene Sequence

>Msa0299220

ATGAAAAAGAACTGCGAACTCTGCAAGCTTCCAGCTAGAACTTTCTGTGAGTCCGACCAGGCTAGCTTATGTTGGGACTGCGATTCTAAGGTCCACGCTGCTAACTTTTTGGTTGAGAGACACATGAGAACTCTCCTCTGTCACGCATGTCAATCTCCCACGCCGTGGAAAGCCTCCGGTGCTAGGCTCGGTAACGCGCTTTCGTTGTGTGATAGATGCGCCGGCGGAAGAAAACTCCACGCCAACGCTAACTCCAACGCCGGTGCCAGCGCCGAGCAACAAGAAGAAAGTGAAGGTGACAATGACAATGAAGAAGACACCGATTACGATTCCGATGAGGAAGATGAGGACGAGGATGAAGAAGATGGTGATAATCAGGTTGTGCCGTGGTCATCAACAGCGGCGCAACCACCACCGGCTTCGAGTTCTTCCGGTAGCGATGAGTCGGTTACGGTTAGCAAGTGCAACGATAACCACGATGAGGTTATTTCTAAGTTAGTTACAACGATTTCTTTGAAACGACGTCGTTTAGATCATGATTTTCAGGTTACGGATTCAAAAAATTGGAAAAATCAACGAAGAGAGGTGGATGAGGTTGGTTTGATAGGGTGCGACGAGGAACCGTCGTCAAAGGCGGCGATGCGGCGGTATAGCGACGTAAATGGAACGGAGCATTCTCAGCCACACGATTGA
Protein sequence

>Msa0299220

MKKNCELCKLPARTFCESDQASLCWDCDSKVHAANFLVERHMRTLLCHACQSPTPWKASGARLGNALSLCDRCAGGRKLHANANSNAGASAEQQEESEGDNDNEEDTDYDSDEEDEDEDEEDGDNQVVPWSSTAAQPPPASSSSGSDESVTVSKCNDNHDEVISKLVTTISLKRRRLDHDFQVTDSKNWKNQRREVDEVGLIGCDEEPSSKAAMRRYSDVNGTEHSQPHD*