AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0219920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0219920 MtrunA17_Chr2g0323271 90.129 233 20 2 1 230 1 233 4.75e-122 346
Msa0219920 MtrunA17_Chr2g0280901 47.917 144 48 2 1 144 1 117 1.85e-31 112
Msa0219920 MtrunA17_Chr2g0322971 52.542 59 28 0 3 61 29 87 1.60e-13 65.1
Msa0219920 MtrunA17_Chr4g0024021 50.847 59 29 0 5 63 36 94 1.01e-12 63.2
Msa0219920 MtrunA17_Chr4g0002211 50.000 66 33 0 3 68 6 71 2.37e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0219920 AT4G27310.1 42.197 173 56 3 1 138 1 164 7.30e-36 126
Msa0219920 AT5G54470.1 47.059 187 78 4 2 188 3 168 5.34e-34 121
Msa0219920 AT3G21890.1 56.000 50 22 0 5 54 31 80 8.33e-11 58.2

Find 73 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCGGTATAGCGACGTAAA+TGG 0.268815 2_1:+67895693 Msa0219920:CDS
TTCACTTTCTTCTTGTTGCT+CGG 0.346480 2_1:-67895215 None:intergenic
ATGCTGGGACTGCGATTCTA+AGG 0.346631 2_1:+67895012 Msa0219920:CDS
TTCTTCTTGTTGCTCGGCGC+TGG 0.356031 2_1:-67895209 None:intergenic
GTGGATGAGGTTGATTTGAT+AGG 0.378300 2_1:+67895636 Msa0219920:CDS
TTACAAAATTAATAATCATA+TGG 0.382752 2_1:-67896031 None:intergenic
ACGAAACCGCGTTGAGAGAA+AGG 0.398885 2_1:+67894901 Msa0219920:five_prime_UTR
TGAAGAAGATGGTGATAATC+AGG 0.403542 2_1:+67895300 Msa0219920:CDS
TTCCTCATCGGAATCGTAAT+CGG 0.408569 2_1:-67895263 None:intergenic
CTCGGCGCTGGCACCGGCGT+TGG 0.429781 2_1:-67895197 None:intergenic
TTTCTTGTGATGGTTTGGTA+TGG 0.436961 2_1:-67894798 None:intergenic
CGTGGAAAGCCTCCGGCGCT+AGG 0.440306 2_1:+67895107 Msa0219920:CDS
TTGTTGCTCGGCGCTGGCAC+CGG 0.453269 2_1:-67895203 None:intergenic
GATTATGGTTGTGGCCTTGT+GGG 0.456916 2_1:+67895897 Msa0219920:three_prime_UTR
CGTCGTCAAAGGCGGCGATT+CGG 0.463533 2_1:+67895673 Msa0219920:CDS
TGTTTATATAACTTAGGTAA+AGG 0.465810 2_1:+67895976 Msa0219920:three_prime_UTR
CGAATCGCCGCCTTTGACGA+CGG 0.469995 2_1:-67895672 None:intergenic
CCGACCAGGCTAGCTTATGC+TGG 0.471766 2_1:+67894996 Msa0219920:CDS
GGTTTGGTATGGATGAAGAA+TGG 0.486262 2_1:-67894787 None:intergenic
GTTTGGTATGGATGAAGAAT+GGG 0.493335 2_1:-67894786 None:intergenic
CGCGTTACCGAGCCTAGCGC+CGG 0.498391 2_1:-67895119 None:intergenic
GCCAACGCTAACTCCAACGC+CGG 0.517678 2_1:+67895184 Msa0219920:CDS
ATAATATCTCTAATATAGTA+CGG 0.521383 2_1:-67895746 None:intergenic
CTCGTCCTCATCTTCCTCAT+CGG 0.523137 2_1:-67895275 None:intergenic
CCCACGCCGTGGAAAGCCTC+CGG 0.526413 2_1:+67895100 Msa0219920:CDS
GACTCACAGAAAGTTCTAGC+TGG 0.526494 2_1:-67894974 None:intergenic
AGTCCCAGCATAAGCTAGCC+TGG 0.526991 2_1:-67895000 None:intergenic
ACCGGCGTTGGAGTTAGCGT+TGG 0.527253 2_1:-67895185 None:intergenic
GAGGACGAGGATGAAGAAGA+TGG 0.529527 2_1:+67895289 Msa0219920:CDS
AACTTTCTGTGAGTCCGACC+AGG 0.531925 2_1:+67894982 Msa0219920:CDS
CCACCGGCTTCTAGTTCCTC+CGG 0.538845 2_1:+67895355 Msa0219920:CDS
TGGATGAGGTTGATTTGATA+GGG 0.541461 2_1:+67895637 Msa0219920:CDS
GCCGGAGGCTTTCCACGGCG+TGG 0.543692 2_1:-67895101 None:intergenic
CGATGAGGAAGATGAGGACG+AGG 0.544127 2_1:+67895276 Msa0219920:CDS
CGACGAGGAACCGTCGTCAA+AGG 0.547277 2_1:+67895662 Msa0219920:CDS
TTCCTCCGGTAGCGAAGAGT+CGG 0.547879 2_1:+67895369 Msa0219920:CDS
AAAGCCTCCGGCGCTAGGCT+CGG 0.551924 2_1:+67895112 Msa0219920:CDS
AACAGCGGCGCAACCACCAC+CGG 0.553693 2_1:+67895339 Msa0219920:CDS
CGACCAGGCTAGCTTATGCT+GGG 0.557426 2_1:+67894997 Msa0219920:CDS
GTTACCGAGCCTAGCGCCGG+AGG 0.559866 2_1:-67895116 None:intergenic
CGTCAAAGGCGGCGATTCGG+CGG 0.566085 2_1:+67895676 Msa0219920:CDS
GGATTATGGTTGTGGCCTTG+TGG 0.566677 2_1:+67895896 Msa0219920:three_prime_UTR
CCGGAGGCTTTCCACGGCGT+GGG 0.569147 2_1:-67895100 None:intergenic
CATTCTCAGCCACACGATTG+AGG 0.570452 2_1:+67895723 Msa0219920:CDS
CTAGCGCCGGAGGCTTTCCA+CGG 0.572274 2_1:-67895106 None:intergenic
CGTAACCGACTCTTCGCTAC+CGG 0.573854 2_1:-67895374 None:intergenic
TCAACGAAGAGAGGTGGATG+AGG 0.575020 2_1:+67895623 Msa0219920:CDS
AGAATGGGTAAGAAGAGGAA+AGG 0.576671 2_1:-67894771 None:intergenic
CGTTGGAGTTAGCGTTGGCG+TGG 0.586469 2_1:-67895180 None:intergenic
TCGTTGTGTGATAGATGCGC+CGG 0.591718 2_1:+67895145 Msa0219920:CDS
GATGAAGAATGGGTAAGAAG+AGG 0.601495 2_1:-67894776 None:intergenic
CCAGCATAAGCTAGCCTGGT+CGG 0.605872 2_1:-67894996 None:intergenic
CGGTAGCGAAGAGTCGGTTA+CGG 0.606860 2_1:+67895375 Msa0219920:CDS
CGAGGAACCGTCGTCAAAGG+CGG 0.613549 2_1:+67895665 Msa0219920:CDS
CTACCGGAGGAACTAGAAGC+CGG 0.616312 2_1:-67895358 None:intergenic
CGATTCCGATGAGGAAGATG+AGG 0.616964 2_1:+67895270 Msa0219920:CDS
GAGGAACTAGAAGCCGGTGG+TGG 0.618905 2_1:-67895352 None:intergenic
GAGATTGACATGCGTGACAG+AGG 0.627483 2_1:-67895078 None:intergenic
GTATAGCGACGTAAATGGAA+CGG 0.632422 2_1:+67895698 Msa0219920:CDS
AACCGACTCTTCGCTACCGG+AGG 0.640395 2_1:-67895371 None:intergenic
CATGTCAATCTCCCACGCCG+TGG 0.644127 2_1:+67895089 Msa0219920:CDS
ATAGTACGGCCTCAATCGTG+TGG 0.647407 2_1:-67895732 None:intergenic
GTGATAATCAGGTTGTTCCG+TGG 0.650569 2_1:+67895311 Msa0219920:CDS
TACTAAGCTAATCACCCACA+AGG 0.661266 2_1:-67895911 None:intergenic
TTGTGTGATAGATGCGCCGG+CGG 0.662123 2_1:+67895148 Msa0219920:CDS
GAGCAACAAGAAGAAAGTGA+AGG 0.680813 2_1:+67895217 Msa0219920:CDS
TGCGCCGCTGTTGATGACCA+CGG 0.694509 2_1:-67895328 None:intergenic
CACCGATTACGATTCCGATG+AGG 0.696165 2_1:+67895261 Msa0219920:CDS
CCGGAGGAACTAGAAGCCGG+TGG 0.696282 2_1:-67895355 None:intergenic
TGATTTGATAGGGTGCGACG+AGG 0.704245 2_1:+67895647 Msa0219920:CDS
TGTTCCGTGGTCATCAACAG+CGG 0.708011 2_1:+67895324 Msa0219920:CDS
GTGCAACGATAACCACGATG+AGG 0.723607 2_1:+67895405 Msa0219920:CDS
ACTTAGAAATAACCTCATCG+TGG 0.742114 2_1:-67895417 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACAAAATTAATAATCATA+TGG - chr2_1:67896034-67896053 None:intergenic 10.0%
!! TTACAAAATTAATAATCATA+TGG - chr2_1:67896034-67896053 None:intergenic 10.0%
!! ATAATATCTCTAATATAGTA+CGG - chr2_1:67895749-67895768 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG + chr2_1:67895765-67895784 Msa0219920:three_prime_UTR 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG + chr2_1:67895875-67895894 Msa0219920:three_prime_UTR 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG + chr2_1:67895970-67895989 Msa0219920:three_prime_UTR 15.0%
!! ATAATATCTCTAATATAGTA+CGG - chr2_1:67895749-67895768 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG + chr2_1:67895765-67895784 Msa0219920:three_prime_UTR 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG + chr2_1:67895875-67895894 Msa0219920:three_prime_UTR 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG + chr2_1:67895970-67895989 Msa0219920:three_prime_UTR 15.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG - chr2_1:67894872-67894891 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG + chr2_1:67895575-67895594 Msa0219920:intron 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG + chr2_1:67895882-67895901 Msa0219920:three_prime_UTR 20.0%
!! TGTTTATATAACTTAGGTAA+AGG + chr2_1:67895976-67895995 Msa0219920:three_prime_UTR 20.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG - chr2_1:67894872-67894891 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG + chr2_1:67895575-67895594 Msa0219920:intron 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG + chr2_1:67895882-67895901 Msa0219920:three_prime_UTR 20.0%
!! TGTTTATATAACTTAGGTAA+AGG + chr2_1:67895976-67895995 Msa0219920:three_prime_UTR 20.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG + chr2_1:67895581-67895600 Msa0219920:intron 25.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG + chr2_1:67895581-67895600 Msa0219920:intron 25.0%
! TCGTTTAGATCATGATTTTC+AGG + chr2_1:67895468-67895487 Msa0219920:CDS 30.0%
AGGTTACGGATTCAAAAAAT+TGG + chr2_1:67895595-67895614 Msa0219920:intron 30.0%
!! TATTTTGAGGATTATGGTTG+TGG + chr2_1:67895888-67895907 Msa0219920:three_prime_UTR 30.0%
! TCGTTTAGATCATGATTTTC+AGG + chr2_1:67895468-67895487 Msa0219920:CDS 30.0%
AGGTTACGGATTCAAAAAAT+TGG + chr2_1:67895595-67895614 Msa0219920:intron 30.0%
!! TATTTTGAGGATTATGGTTG+TGG + chr2_1:67895888-67895907 Msa0219920:three_prime_UTR 30.0%
GTTTGGTATGGATGAAGAAT+GGG - chr2_1:67894789-67894808 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG - chr2_1:67894801-67894820 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG - chr2_1:67894806-67894825 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG - chr2_1:67894811-67894830 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG - chr2_1:67894941-67894960 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG - chr2_1:67895218-67895237 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG + chr2_1:67895300-67895319 Msa0219920:CDS 35.0%
ACTTAGAAATAACCTCATCG+TGG - chr2_1:67895420-67895439 None:intergenic 35.0%
TTGGAAAAATCAACGAAGAG+AGG + chr2_1:67895614-67895633 Msa0219920:CDS 35.0%
!! TGGATGAGGTTGATTTGATA+GGG + chr2_1:67895637-67895656 Msa0219920:CDS 35.0%
GTTTGGTATGGATGAAGAAT+GGG - chr2_1:67894789-67894808 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG - chr2_1:67894801-67894820 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG - chr2_1:67894806-67894825 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG - chr2_1:67894811-67894830 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG - chr2_1:67894941-67894960 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG - chr2_1:67895218-67895237 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG + chr2_1:67895300-67895319 Msa0219920:CDS 35.0%
ACTTAGAAATAACCTCATCG+TGG - chr2_1:67895420-67895439 None:intergenic 35.0%
TTGGAAAAATCAACGAAGAG+AGG + chr2_1:67895614-67895633 Msa0219920:CDS 35.0%
!! TGGATGAGGTTGATTTGATA+GGG + chr2_1:67895637-67895656 Msa0219920:CDS 35.0%
GATGAAGAATGGGTAAGAAG+AGG - chr2_1:67894779-67894798 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG - chr2_1:67894790-67894809 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG - chr2_1:67894910-67894929 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG + chr2_1:67895217-67895236 Msa0219920:CDS 40.0%
TTCCTCATCGGAATCGTAAT+CGG - chr2_1:67895266-67895285 None:intergenic 40.0%
GAAAAATCAACGAAGAGAGG+TGG + chr2_1:67895617-67895636 Msa0219920:CDS 40.0%
!! GTGGATGAGGTTGATTTGAT+AGG + chr2_1:67895636-67895655 Msa0219920:CDS 40.0%
GTATAGCGACGTAAATGGAA+CGG + chr2_1:67895698-67895717 Msa0219920:CDS 40.0%
TACTAAGCTAATCACCCACA+AGG - chr2_1:67895914-67895933 None:intergenic 40.0%
GATGAAGAATGGGTAAGAAG+AGG - chr2_1:67894779-67894798 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG - chr2_1:67894790-67894809 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG - chr2_1:67894910-67894929 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG + chr2_1:67895217-67895236 Msa0219920:CDS 40.0%
TTCCTCATCGGAATCGTAAT+CGG - chr2_1:67895266-67895285 None:intergenic 40.0%
GAAAAATCAACGAAGAGAGG+TGG + chr2_1:67895617-67895636 Msa0219920:CDS 40.0%
!! GTGGATGAGGTTGATTTGAT+AGG + chr2_1:67895636-67895655 Msa0219920:CDS 40.0%
GTATAGCGACGTAAATGGAA+CGG + chr2_1:67895698-67895717 Msa0219920:CDS 40.0%
TACTAAGCTAATCACCCACA+AGG - chr2_1:67895914-67895933 None:intergenic 40.0%
! GACTCACAGAAAGTTCTAGC+TGG - chr2_1:67894977-67894996 None:intergenic 45.0%
! GGTTCACGCTGCTAACTTTT+TGG + chr2_1:67895033-67895052 Msa0219920:CDS 45.0%
! GTGATAATCAGGTTGTTCCG+TGG + chr2_1:67895311-67895330 Msa0219920:CDS 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG + chr2_1:67895897-67895916 Msa0219920:three_prime_UTR 45.0%
! GACTCACAGAAAGTTCTAGC+TGG - chr2_1:67894977-67894996 None:intergenic 45.0%
! GGTTCACGCTGCTAACTTTT+TGG + chr2_1:67895033-67895052 Msa0219920:CDS 45.0%
! GTGATAATCAGGTTGTTCCG+TGG + chr2_1:67895311-67895330 Msa0219920:CDS 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG + chr2_1:67895897-67895916 Msa0219920:three_prime_UTR 45.0%
ACGAAACCGCGTTGAGAGAA+AGG + chr2_1:67894901-67894920 Msa0219920:five_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG + chr2_1:67894982-67895001 Msa0219920:CDS 50.0%
ATGCTGGGACTGCGATTCTA+AGG + chr2_1:67895012-67895031 Msa0219920:CDS 50.0%
GAGATTGACATGCGTGACAG+AGG - chr2_1:67895081-67895100 None:intergenic 50.0%
TCGTTGTGTGATAGATGCGC+CGG + chr2_1:67895145-67895164 Msa0219920:CDS 50.0%
! CACCGATTACGATTCCGATG+AGG + chr2_1:67895261-67895280 Msa0219920:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG + chr2_1:67895270-67895289 Msa0219920:CDS 50.0%
CTCGTCCTCATCTTCCTCAT+CGG - chr2_1:67895278-67895297 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG + chr2_1:67895289-67895308 Msa0219920:CDS 50.0%
TGTTCCGTGGTCATCAACAG+CGG + chr2_1:67895324-67895343 Msa0219920:CDS 50.0%
GTGCAACGATAACCACGATG+AGG + chr2_1:67895405-67895424 Msa0219920:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG + chr2_1:67895623-67895642 Msa0219920:CDS 50.0%
TGATTTGATAGGGTGCGACG+AGG + chr2_1:67895647-67895666 Msa0219920:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG + chr2_1:67895723-67895742 Msa0219920:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG - chr2_1:67895735-67895754 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG + chr2_1:67895896-67895915 Msa0219920:three_prime_UTR 50.0%
ACGAAACCGCGTTGAGAGAA+AGG + chr2_1:67894901-67894920 Msa0219920:five_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG + chr2_1:67894982-67895001 Msa0219920:CDS 50.0%
ATGCTGGGACTGCGATTCTA+AGG + chr2_1:67895012-67895031 Msa0219920:CDS 50.0%
GAGATTGACATGCGTGACAG+AGG - chr2_1:67895081-67895100 None:intergenic 50.0%
TCGTTGTGTGATAGATGCGC+CGG + chr2_1:67895145-67895164 Msa0219920:CDS 50.0%
! CACCGATTACGATTCCGATG+AGG + chr2_1:67895261-67895280 Msa0219920:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG + chr2_1:67895270-67895289 Msa0219920:CDS 50.0%
CTCGTCCTCATCTTCCTCAT+CGG - chr2_1:67895278-67895297 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG + chr2_1:67895289-67895308 Msa0219920:CDS 50.0%
TGTTCCGTGGTCATCAACAG+CGG + chr2_1:67895324-67895343 Msa0219920:CDS 50.0%
GTGCAACGATAACCACGATG+AGG + chr2_1:67895405-67895424 Msa0219920:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG + chr2_1:67895623-67895642 Msa0219920:CDS 50.0%
TGATTTGATAGGGTGCGACG+AGG + chr2_1:67895647-67895666 Msa0219920:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG + chr2_1:67895723-67895742 Msa0219920:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG - chr2_1:67895735-67895754 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG + chr2_1:67895896-67895915 Msa0219920:three_prime_UTR 50.0%
! CCAGCATAAGCTAGCCTGGT+CGG - chr2_1:67894999-67895018 None:intergenic 55.0%
!! CGACCAGGCTAGCTTATGCT+GGG + chr2_1:67894997-67895016 Msa0219920:CDS 55.0%
AGTCCCAGCATAAGCTAGCC+TGG - chr2_1:67895003-67895022 None:intergenic 55.0%
TTGTGTGATAGATGCGCCGG+CGG + chr2_1:67895148-67895167 Msa0219920:CDS 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG - chr2_1:67895212-67895231 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG + chr2_1:67895276-67895295 Msa0219920:CDS 55.0%
CTACCGGAGGAACTAGAAGC+CGG - chr2_1:67895361-67895380 None:intergenic 55.0%
TTCCTCCGGTAGCGAAGAGT+CGG + chr2_1:67895369-67895388 Msa0219920:CDS 55.0%
! CGTAACCGACTCTTCGCTAC+CGG - chr2_1:67895377-67895396 None:intergenic 55.0%
CGGTAGCGAAGAGTCGGTTA+CGG + chr2_1:67895375-67895394 Msa0219920:CDS 55.0%
CGGCGGTATAGCGACGTAAA+TGG + chr2_1:67895693-67895712 Msa0219920:CDS 55.0%
! CCAGCATAAGCTAGCCTGGT+CGG - chr2_1:67894999-67895018 None:intergenic 55.0%
!! CGACCAGGCTAGCTTATGCT+GGG + chr2_1:67894997-67895016 Msa0219920:CDS 55.0%
AGTCCCAGCATAAGCTAGCC+TGG - chr2_1:67895003-67895022 None:intergenic 55.0%
TTGTGTGATAGATGCGCCGG+CGG + chr2_1:67895148-67895167 Msa0219920:CDS 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG - chr2_1:67895212-67895231 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG + chr2_1:67895276-67895295 Msa0219920:CDS 55.0%
CTACCGGAGGAACTAGAAGC+CGG - chr2_1:67895361-67895380 None:intergenic 55.0%
TTCCTCCGGTAGCGAAGAGT+CGG + chr2_1:67895369-67895388 Msa0219920:CDS 55.0%
! CGTAACCGACTCTTCGCTAC+CGG - chr2_1:67895377-67895396 None:intergenic 55.0%
CGGTAGCGAAGAGTCGGTTA+CGG + chr2_1:67895375-67895394 Msa0219920:CDS 55.0%
CGGCGGTATAGCGACGTAAA+TGG + chr2_1:67895693-67895712 Msa0219920:CDS 55.0%
!! CCGACCAGGCTAGCTTATGC+TGG + chr2_1:67894996-67895015 Msa0219920:CDS 60.0%
CATGTCAATCTCCCACGCCG+TGG + chr2_1:67895089-67895108 Msa0219920:CDS 60.0%
! GGCGTGGAGTTTTCTTCCGC+CGG - chr2_1:67895167-67895186 None:intergenic 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG - chr2_1:67895183-67895202 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG + chr2_1:67895184-67895203 Msa0219920:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG - chr2_1:67895188-67895207 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG - chr2_1:67895331-67895350 None:intergenic 60.0%
GAGGAACTAGAAGCCGGTGG+TGG - chr2_1:67895355-67895374 None:intergenic 60.0%
!! CCACCGGCTTCTAGTTCCTC+CGG + chr2_1:67895355-67895374 Msa0219920:CDS 60.0%
! AACCGACTCTTCGCTACCGG+AGG - chr2_1:67895374-67895393 None:intergenic 60.0%
CGACGAGGAACCGTCGTCAA+AGG + chr2_1:67895662-67895681 Msa0219920:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG + chr2_1:67895665-67895684 Msa0219920:CDS 60.0%
CGAATCGCCGCCTTTGACGA+CGG - chr2_1:67895675-67895694 None:intergenic 60.0%
CGTCGTCAAAGGCGGCGATT+CGG + chr2_1:67895673-67895692 Msa0219920:CDS 60.0%
!! CCGACCAGGCTAGCTTATGC+TGG + chr2_1:67894996-67895015 Msa0219920:CDS 60.0%
CATGTCAATCTCCCACGCCG+TGG + chr2_1:67895089-67895108 Msa0219920:CDS 60.0%
! GGCGTGGAGTTTTCTTCCGC+CGG - chr2_1:67895167-67895186 None:intergenic 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG - chr2_1:67895183-67895202 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG + chr2_1:67895184-67895203 Msa0219920:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG - chr2_1:67895188-67895207 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG - chr2_1:67895331-67895350 None:intergenic 60.0%
GAGGAACTAGAAGCCGGTGG+TGG - chr2_1:67895355-67895374 None:intergenic 60.0%
!! CCACCGGCTTCTAGTTCCTC+CGG + chr2_1:67895355-67895374 Msa0219920:CDS 60.0%
! AACCGACTCTTCGCTACCGG+AGG - chr2_1:67895374-67895393 None:intergenic 60.0%
CGACGAGGAACCGTCGTCAA+AGG + chr2_1:67895662-67895681 Msa0219920:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG + chr2_1:67895665-67895684 Msa0219920:CDS 60.0%
CGAATCGCCGCCTTTGACGA+CGG - chr2_1:67895675-67895694 None:intergenic 60.0%
CGTCGTCAAAGGCGGCGATT+CGG + chr2_1:67895673-67895692 Msa0219920:CDS 60.0%
! CTAGCGCCGGAGGCTTTCCA+CGG - chr2_1:67895109-67895128 None:intergenic 65.0%
AAAGCCTCCGGCGCTAGGCT+CGG + chr2_1:67895112-67895131 Msa0219920:CDS 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG - chr2_1:67895206-67895225 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG + chr2_1:67895339-67895358 Msa0219920:CDS 65.0%
CCGGAGGAACTAGAAGCCGG+TGG - chr2_1:67895358-67895377 None:intergenic 65.0%
! CGTCAAAGGCGGCGATTCGG+CGG + chr2_1:67895676-67895695 Msa0219920:CDS 65.0%
! CTAGCGCCGGAGGCTTTCCA+CGG - chr2_1:67895109-67895128 None:intergenic 65.0%
AAAGCCTCCGGCGCTAGGCT+CGG + chr2_1:67895112-67895131 Msa0219920:CDS 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG - chr2_1:67895206-67895225 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG + chr2_1:67895339-67895358 Msa0219920:CDS 65.0%
CCGGAGGAACTAGAAGCCGG+TGG - chr2_1:67895358-67895377 None:intergenic 65.0%
! CGTCAAAGGCGGCGATTCGG+CGG + chr2_1:67895676-67895695 Msa0219920:CDS 65.0%
CCGGAGGCTTTCCACGGCGT+GGG - chr2_1:67895103-67895122 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG + chr2_1:67895100-67895119 Msa0219920:CDS 70.0%
CGTGGAAAGCCTCCGGCGCT+AGG + chr2_1:67895107-67895126 Msa0219920:CDS 70.0%
! GTTACCGAGCCTAGCGCCGG+AGG - chr2_1:67895119-67895138 None:intergenic 70.0%
CGCGTTACCGAGCCTAGCGC+CGG - chr2_1:67895122-67895141 None:intergenic 70.0%
CCGGAGGCTTTCCACGGCGT+GGG - chr2_1:67895103-67895122 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG + chr2_1:67895100-67895119 Msa0219920:CDS 70.0%
CGTGGAAAGCCTCCGGCGCT+AGG + chr2_1:67895107-67895126 Msa0219920:CDS 70.0%
! GTTACCGAGCCTAGCGCCGG+AGG - chr2_1:67895119-67895138 None:intergenic 70.0%
CGCGTTACCGAGCCTAGCGC+CGG - chr2_1:67895122-67895141 None:intergenic 70.0%
GCCGGAGGCTTTCCACGGCG+TGG - chr2_1:67895104-67895123 None:intergenic 75.0%
GCCGGAGGCTTTCCACGGCG+TGG - chr2_1:67895104-67895123 None:intergenic 75.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG - chr2_1:67895200-67895219 None:intergenic 80.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG - chr2_1:67895200-67895219 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 67894773 67896089 67894773 ID=Msa0219920;Name=Msa0219920
chr2_1 mRNA 67894773 67896089 67894773 ID=Msa0219920-mRNA-1;Parent=Msa0219920;Name=Msa0219920-mRNA-1;_AED=0.00;_eAED=0.00;_QI=171|1|1|1|1|1|2|346|230
chr2_1 exon 67894773 67895489 67894773 ID=Msa0219920-mRNA-1:exon:19221;Parent=Msa0219920-mRNA-1
chr2_1 exon 67895597 67896089 67895597 ID=Msa0219920-mRNA-1:exon:19222;Parent=Msa0219920-mRNA-1
chr2_1 five_prime_UTR 67894773 67894943 67894773 ID=Msa0219920-mRNA-1:five_prime_utr;Parent=Msa0219920-mRNA-1
chr2_1 CDS 67894944 67895489 67894944 ID=Msa0219920-mRNA-1:cds;Parent=Msa0219920-mRNA-1
chr2_1 CDS 67895597 67895743 67895597 ID=Msa0219920-mRNA-1:cds;Parent=Msa0219920-mRNA-1
chr2_1 three_prime_UTR 67895744 67896089 67895744 ID=Msa0219920-mRNA-1:three_prime_utr;Parent=Msa0219920-mRNA-1
Gene Sequence

>Msa0219920

ATGAAAAAGAACTGCGAACTCTGCAAGCTTCCAGCTAGAACTTTCTGTGAGTCCGACCAGGCTAGCTTATGCTGGGACTGCGATTCTAAGGTTCACGCTGCTAACTTTTTGGTTGAGAGACACATGAGAACTCTCCTCTGTCACGCATGTCAATCTCCCACGCCGTGGAAAGCCTCCGGCGCTAGGCTCGGTAACGCGCTTTCGTTGTGTGATAGATGCGCCGGCGGAAGAAAACTCCACGCCAACGCTAACTCCAACGCCGGTGCCAGCGCCGAGCAACAAGAAGAAAGTGAAGGTGACAATGACAATGAAGAAGACACCGATTACGATTCCGATGAGGAAGATGAGGACGAGGATGAAGAAGATGGTGATAATCAGGTTGTTCCGTGGTCATCAACAGCGGCGCAACCACCACCGGCTTCTAGTTCCTCCGGTAGCGAAGAGTCGGTTACGGTTAGCAAGTGCAACGATAACCACGATGAGGTTATTTCTAAGTTAGTTACAACGATTTCTTTGAAACGACGTCGTTTAGATCATGATTTTCAGGTTACGGATTCAAAAAATTGGAAAAATCAACGAAGAGAGGTGGATGAGGTTGATTTGATAGGGTGCGACGAGGAACCGTCGTCAAAGGCGGCGATTCGGCGGTATAGCGACGTAAATGGAACGGAGCATTCTCAGCCACACGATTGA
Protein sequence

>Msa0219920

MKKNCELCKLPARTFCESDQASLCWDCDSKVHAANFLVERHMRTLLCHACQSPTPWKASGARLGNALSLCDRCAGGRKLHANANSNAGASAEQQEESEGDNDNEEDTDYDSDEEDEDEDEEDGDNQVVPWSSTAAQPPPASSSSGSEESVTVSKCNDNHDEVISKLVTTISLKRRRLDHDFQVTDSKNWKNQRREVDEVDLIGCDEEPSSKAAIRRYSDVNGTEHSQPHD*