AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0261640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261640 MtrunA17_Chr2g0324021 95.455 264 12 0 1 264 1 264 0.0 515
Msa0261640 MtrunA17_Chr7g0270441 81.579 266 47 2 1 264 1 266 6.26e-155 432
Msa0261640 MtrunA17_Chr7g0270451 69.349 261 79 1 1 261 1 260 1.20e-130 370
Msa0261640 MtrunA17_Chr7g0270471 83.784 148 24 0 112 259 1 148 4.21e-86 253
Msa0261640 MtrunA17_Chr6g0459261 50.598 251 122 2 5 253 24 274 9.57e-86 257
Msa0261640 MtrunA17_Chr7g0266981 50.980 255 123 2 3 256 10 263 9.12e-82 246
Msa0261640 MtrunA17_Chr7g0244061 50.179 279 115 5 2 259 25 300 3.68e-81 246
Msa0261640 MtrunA17_Chr6g0459351 50.593 253 123 2 2 252 9 261 5.88e-81 244
Msa0261640 MtrunA17_Chr6g0459331 50.593 253 123 2 5 255 12 264 9.74e-81 243
Msa0261640 MtrunA17_Chr6g0459281 51.600 250 119 2 5 252 12 261 1.06e-80 244
Msa0261640 MtrunA17_Chr4g0044111 50.588 255 124 2 3 256 10 263 1.65e-80 243
Msa0261640 MtrunA17_Chr6g0459271 49.004 251 126 2 5 253 12 262 1.34e-78 238
Msa0261640 MtrunA17_Chr6g0459301 47.244 254 132 2 2 253 10 263 2.46e-78 238
Msa0261640 MtrunA17_Chr4g0026001 50.588 255 121 3 4 257 12 262 2.70e-78 238
Msa0261640 MtrunA17_Chr7g0266891 50.000 252 124 2 6 256 7 257 7.12e-78 236
Msa0261640 MtrunA17_Chr4g0025971 50.196 255 120 4 4 256 12 261 1.73e-77 236
Msa0261640 MtrunA17_Chr4g0025981 50.598 251 119 4 7 256 42 288 1.76e-75 232
Msa0261640 MtrunA17_Chr6g0463951 63.158 171 63 0 87 257 1 171 2.17e-72 219
Msa0261640 MtrunA17_Chr4g0044131 47.328 262 135 3 4 263 6 266 3.05e-72 222
Msa0261640 MtrunA17_Chr7g0268591 45.312 256 132 4 4 253 31 284 6.82e-71 219
Msa0261640 MtrunA17_Chr7g0252031 45.865 266 134 6 4 261 33 296 3.48e-70 218
Msa0261640 MtrunA17_Chr4g0040371 43.223 273 142 5 1 264 14 282 1.84e-64 202
Msa0261640 MtrunA17_Chr7g0267001 45.211 261 124 4 4 263 6 248 1.06e-62 197
Msa0261640 MtrunA17_Chr3g0084871 43.494 269 141 5 4 263 14 280 4.98e-62 196
Msa0261640 MtrunA17_Chr7g0267011 48.677 189 95 2 4 191 6 193 5.98e-54 173
Msa0261640 MtrunA17_Chr7g0270461 84.946 93 14 0 4 96 44 136 1.08e-50 163
Msa0261640 MtrunA17_Chr7g0266991 42.424 231 114 3 4 233 23 235 5.52e-49 162
Msa0261640 MtrunA17_Chr4g0044121 47.953 171 87 2 87 256 1 170 4.97e-44 147
Msa0261640 MtrunA17_Chr3g0142651 48.718 117 58 2 87 202 1 116 8.66e-31 111
Msa0261640 MtrunA17_Chr4g0028921 30.909 275 152 6 1 251 1 261 9.02e-26 102
Msa0261640 MtrunA17_Chr4g0028911 29.856 278 157 6 1 254 1 264 1.45e-22 93.6
Msa0261640 MtrunA17_Chr3g0122901 27.132 258 167 9 4 252 73 318 3.99e-21 90.5
Msa0261640 MtrunA17_Chr4g0074511 27.863 262 163 8 5 251 39 289 1.93e-20 88.2
Msa0261640 MtrunA17_Chr7g0272961 26.316 266 170 7 5 256 39 292 3.46e-20 87.8
Msa0261640 MtrunA17_Chr1g0191751 29.537 281 142 12 5 256 100 353 9.99e-20 87.4
Msa0261640 MtrunA17_Chr4g0075531 28.175 252 170 5 4 252 5 248 1.56e-19 85.1
Msa0261640 MtrunA17_Chr0c30g0494101 27.757 263 160 9 5 251 39 287 3.77e-18 82.0
Msa0261640 MtrunA17_Chr1g0171831 31.122 196 123 4 4 187 12 207 3.91e-16 75.9
Msa0261640 MtrunA17_Chr3g0112151 26.357 258 178 7 2 254 82 332 4.45e-15 73.9
Msa0261640 MtrunA17_Chr3g0112171 27.341 267 173 8 6 263 15 269 8.73e-15 72.4
Msa0261640 MtrunA17_Chr3g0112141 26.482 253 174 7 6 253 17 262 9.68e-15 72.4
Msa0261640 MtrunA17_Chr8g0357481 28.358 268 172 10 6 260 14 274 1.25e-14 72.4
Msa0261640 MtrunA17_Chr3g0112271 28.839 267 179 9 1 263 12 271 5.88e-14 70.1
Msa0261640 MtrunA17_Chr7g0239491 28.736 261 169 9 2 253 11 263 6.36e-14 69.7
Msa0261640 MtrunA17_Chr4g0045061 27.203 261 149 7 8 248 6 245 1.76e-13 70.1
Msa0261640 MtrunA17_Chr7g0259531 30.078 256 163 8 6 252 11 259 2.21e-13 68.2
Msa0261640 MtrunA17_Chr3g0112261 27.626 257 176 9 3 255 16 266 3.03e-13 68.2
Msa0261640 MtrunA17_Chr4g0045041 27.536 207 128 4 8 197 6 207 9.23e-13 67.8
Msa0261640 MtrunA17_Chr3g0124481 33.094 139 87 3 56 192 59 193 9.54e-13 66.6
Msa0261640 MtrunA17_Chr4g0054341 27.225 191 131 5 7 194 3 188 1.32e-12 66.2
Msa0261640 MtrunA17_Chr7g0239481 29.771 262 164 9 2 252 11 263 3.20e-12 65.1
Msa0261640 MtrunA17_Chr7g0239501 27.376 263 173 9 2 254 12 266 1.17e-11 63.5
Msa0261640 MtrunA17_Chr4g0032181 24.324 222 151 6 38 254 38 247 3.29e-11 62.0
Msa0261640 MtrunA17_Chr4g0032141 26.070 257 178 8 3 254 53 302 5.34e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261640 AT2G47140.1 61.176 255 99 0 1 255 1 255 1.56e-111 322
Msa0261640 AT3G29250.2 58.462 260 100 2 1 256 1 256 2.35e-102 299
Msa0261640 AT2G47130.1 58.687 259 100 2 1 256 1 255 2.61e-102 298
Msa0261640 AT3G29250.3 58.462 260 100 2 1 256 37 292 6.72e-102 299
Msa0261640 AT3G29250.1 58.140 258 100 2 3 256 41 294 5.24e-101 296
Msa0261640 AT2G47120.1 56.538 260 103 4 1 256 1 254 1.26e-100 294
Msa0261640 AT3G29260.1 57.529 259 103 2 1 256 1 255 1.09e-99 292
Msa0261640 AT2G47120.2 56.641 256 101 4 5 256 6 255 1.19e-99 292
Msa0261640 AT3G51680.1 48.529 272 124 3 4 259 30 301 1.43e-76 234
Msa0261640 AT3G26770.1 43.284 268 140 5 4 261 39 304 1.66e-64 204
Msa0261640 AT4G03140.1 43.798 258 137 4 4 255 76 331 8.09e-63 201
Msa0261640 AT1G52340.1 45.693 267 127 6 4 256 16 278 2.88e-62 197
Msa0261640 AT3G26760.1 40.000 270 150 5 2 261 32 299 5.47e-61 195
Msa0261640 AT3G42960.1 44.061 261 134 3 2 253 4 261 8.03e-55 178
Msa0261640 AT2G47150.1 37.052 251 105 7 6 256 1 198 8.30e-38 132
Msa0261640 AT3G55290.2 28.682 258 165 5 6 251 17 267 2.47e-21 90.9
Msa0261640 AT3G55290.1 28.682 258 165 5 6 251 18 268 2.84e-21 90.9
Msa0261640 AT1G62610.3 29.231 260 162 6 6 251 15 266 2.06e-20 88.6
Msa0261640 AT1G62610.1 29.231 260 162 6 6 251 14 265 2.18e-20 88.2
Msa0261640 AT1G62610.2 29.231 260 162 6 6 251 13 264 2.28e-20 88.2
Msa0261640 AT1G07450.1 28.736 261 169 7 1 254 3 253 2.39e-20 87.8
Msa0261640 AT1G62610.4 29.231 260 162 6 6 251 19 270 2.58e-20 88.2
Msa0261640 AT3G55310.1 28.947 266 154 7 6 251 17 267 4.20e-19 84.7
Msa0261640 AT2G29340.2 28.788 264 162 8 3 254 4 253 9.32e-19 83.6
Msa0261640 AT2G29310.1 27.778 270 157 8 3 254 4 253 9.32e-19 83.6
Msa0261640 AT4G05530.1 30.709 254 161 7 4 252 7 250 1.01e-18 83.2
Msa0261640 AT1G63380.3 27.099 262 171 5 6 254 15 269 1.11e-18 83.6
Msa0261640 AT1G63380.2 27.099 262 171 5 6 254 22 276 1.26e-18 83.6
Msa0261640 AT1G63380.1 27.099 262 171 5 6 254 17 271 1.31e-18 83.6
Msa0261640 AT1G63380.4 27.099 262 171 5 6 254 19 273 1.59e-18 83.2
Msa0261640 AT2G29340.1 28.788 264 162 8 3 254 4 253 2.08e-18 83.2
Msa0261640 AT3G46170.1 26.744 258 170 5 6 251 26 276 2.22e-18 83.2
Msa0261640 AT1G24360.1 25.194 258 176 8 4 254 72 319 1.50e-17 81.3
Msa0261640 AT2G17845.1 27.547 265 170 6 6 255 47 304 3.80e-17 79.7
Msa0261640 AT2G29150.1 28.185 259 171 7 2 254 12 261 5.17e-17 79.0
Msa0261640 AT1G54870.2 26.316 266 170 7 5 256 83 336 6.76e-17 79.3
Msa0261640 AT1G54870.1 26.316 266 170 7 5 256 81 334 8.33e-17 79.3
Msa0261640 AT5G06060.1 29.457 258 168 8 3 254 6 255 2.31e-16 77.0
Msa0261640 AT2G30670.1 26.744 258 175 6 3 254 4 253 2.53e-16 77.0
Msa0261640 AT2G29290.2 27.652 264 165 6 3 254 4 253 2.85e-16 76.6
Msa0261640 AT1G07440.1 27.734 256 174 8 3 254 9 257 5.75e-16 75.9
Msa0261640 AT2G29370.1 27.068 266 176 8 3 260 13 268 4.04e-15 73.6
Msa0261640 AT3G05260.1 27.273 264 166 8 5 254 36 287 4.37e-15 73.9
Msa0261640 AT2G29350.1 27.027 259 174 7 2 254 11 260 4.39e-15 73.6
Msa0261640 AT2G29360.1 27.099 262 169 9 3 254 13 262 6.94e-15 73.2
Msa0261640 AT2G29340.3 30.151 199 128 7 3 195 4 197 2.12e-14 70.5
Msa0261640 AT2G29340.4 30.151 199 128 7 3 195 4 197 2.12e-14 70.5
Msa0261640 AT2G29260.1 29.389 262 167 10 2 254 62 314 2.82e-14 72.0
Msa0261640 AT2G29290.1 28.000 200 138 4 56 254 42 236 3.70e-14 70.5
Msa0261640 AT1G49670.2 30.045 223 125 7 8 210 6 217 3.92e-14 72.4
Msa0261640 AT2G29300.1 28.185 259 171 9 3 254 4 254 4.76e-14 70.5
Msa0261640 AT1G49670.1 30.045 223 125 7 8 210 6 217 5.03e-14 72.0
Msa0261640 AT2G29300.2 27.273 264 177 8 3 259 4 259 5.10e-14 70.9
Msa0261640 AT3G12800.1 28.413 271 171 11 6 261 10 272 6.15e-14 70.5
Msa0261640 AT4G13180.1 31.418 261 157 10 6 254 13 263 1.26e-13 69.3
Msa0261640 AT1G10310.1 33.333 132 86 2 63 194 73 202 1.75e-13 68.6
Msa0261640 AT2G29310.3 28.019 207 122 6 3 195 4 197 6.42e-13 66.2
Msa0261640 AT2G29330.1 26.562 256 178 6 3 254 4 253 9.14e-13 67.0
Msa0261640 AT5G50770.1 28.796 191 128 5 1 187 40 226 2.91e-12 66.2
Msa0261640 AT2G29350.3 26.000 200 141 5 56 254 29 222 5.29e-12 64.3
Msa0261640 AT2G29350.2 27.500 200 134 5 2 195 11 205 1.24e-11 63.2
Msa0261640 AT5G18210.1 28.358 268 163 9 2 252 4 259 1.63e-11 63.2
Msa0261640 AT2G29310.2 25.726 241 141 8 32 254 31 251 1.78e-11 63.2

Find 98 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTGTTGATTTGAAATT+AGG 0.166758 2_2:-60155532 Msa0261640:three_prime_UTR
ATAACACTTCTTATAAAATA+AGG 0.214402 2_2:-60155990 Msa0261640:three_prime_UTR
GTATATTATATTTATAATAT+TGG 0.214598 2_2:-60155112 Msa0261640:three_prime_UTR
TTAATTTGAATAATGATAAA+TGG 0.219286 2_2:-60155509 Msa0261640:three_prime_UTR
GAGACAAAATCTGATTTAAT+TGG 0.223084 2_2:-60155324 Msa0261640:three_prime_UTR
TTCTCTATTTCATGTCGTTT+TGG 0.264368 2_2:-60155846 Msa0261640:three_prime_UTR
TGCAGTGAGCTTGGAGCTTA+TGG 0.278067 2_2:-60156446 Msa0261640:CDS
AGCAGATATCAATGATGAAT+TGG 0.283540 2_2:-60156849 Msa0261640:CDS
TGGTGTTGTCAAATTCATTT+AGG 0.283555 2_2:+60156648 None:intergenic
GCATCAAAACATGGTCTTAT+AGG 0.284802 2_2:-60156485 Msa0261640:CDS
CCTTGATTATGCTAATTTGC+AGG 0.303341 2_2:-60156330 Msa0261640:CDS
CATCATGTTGCAAATTCAAT+AGG 0.308261 2_2:-60156824 Msa0261640:CDS
GTAAAGATATTCGTGGAAAA+TGG 0.310172 2_2:-60156887 Msa0261640:CDS
TAACACTTCTTATAAAATAA+GGG 0.349329 2_2:-60155989 Msa0261640:three_prime_UTR
TACACTGGCAGTGCAAATTA+TGG 0.353051 2_2:+60156547 None:intergenic
GATGAATCTGCTTATGTTAG+TGG 0.370705 2_2:-60156254 Msa0261640:CDS
TTTACAGAGTTTCAAATTGT+AGG 0.375790 2_2:+60155642 None:intergenic
TTTCTGCTTCATTCCTTTCA+CGG 0.389704 2_2:+60155395 None:intergenic
ATGGTCTTATAGGACTTGTT+AGG 0.392057 2_2:-60156475 Msa0261640:CDS
GCTTTCACGTTGGAGAGATA+TGG 0.399630 2_2:-60156737 Msa0261640:CDS
ACTGGGGCAGCAAGCGGAAT+AGG 0.399759 2_2:-60156920 Msa0261640:CDS
AACTGAAATTTAACATATCA+TGG 0.401250 2_2:+60155590 None:intergenic
TTCTGCTTCATTCCTTTCAC+GGG 0.403395 2_2:+60155396 None:intergenic
CAGAAAATTTAACTAGAGAA+TGG 0.410968 2_2:-60155466 Msa0261640:three_prime_UTR
ATTTGCACTGCCAGTGTAGC+TGG 0.419113 2_2:-60156542 Msa0261640:CDS
CAACTTGTTTCTCATCTCTC+AGG 0.421960 2_2:+60156771 None:intergenic
ATTGCTGAACATGATGTCTA+AGG 0.424678 2_2:+60156712 None:intergenic
ATCTATTCACAGGAGGAAAC+AGG 0.428243 2_2:+60155937 None:intergenic
TGATCATAGGTTGGAGGGTA+AGG 0.428592 2_2:-60156954 Msa0261640:intron
ATGATAGAGAAAGCGGTAGA+AGG 0.429802 2_2:+60156149 None:intergenic
AATTCTAGTATGCTAGATAA+AGG 0.435820 2_2:+60156674 None:intergenic
AAATGGTTTGTAAAGAAGAA+TGG 0.436850 2_2:-60155373 Msa0261640:three_prime_UTR
TTATTTAAATGACAAATCAA+TGG 0.437177 2_2:-60155901 Msa0261640:three_prime_UTR
GAATGGGAGAAGGAAGGAAA+AGG 0.439966 2_2:-60155449 Msa0261640:three_prime_UTR
AATGGGAGAAGGAAGGAAAA+GGG 0.440627 2_2:-60155448 Msa0261640:three_prime_UTR
ATCATGTTGCAAATTCAATA+GGG 0.440902 2_2:-60156823 Msa0261640:CDS
AATGGTTTGTAAAGAAGAAT+GGG 0.447185 2_2:-60155372 Msa0261640:three_prime_UTR
GGACACAACTTGGTTGTTGA+TGG 0.453303 2_2:-60156233 Msa0261640:CDS
TTACAGAGTTTCAAATTGTA+GGG 0.454339 2_2:+60155643 None:intergenic
GACATCATGTTCAGCAATGC+TGG 0.459929 2_2:-60156707 Msa0261640:CDS
GCAGATATCAATGATGAATT+GGG 0.463215 2_2:-60156848 Msa0261640:CDS
AGAAAATTTAACTAGAGAAT+GGG 0.466721 2_2:-60155465 Msa0261640:three_prime_UTR
CTAAAATAAGCTATGTGAGT+TGG 0.466968 2_2:-60155203 Msa0261640:three_prime_UTR
ATGATATGTTAAATTTCAGT+TGG 0.474780 2_2:-60155588 Msa0261640:three_prime_UTR
GTGGCTTCTCAGTCATTAAT+CGG 0.474841 2_2:-60156211 Msa0261640:CDS
TTTGTCATTTAAATAAATGA+AGG 0.477552 2_2:+60155908 None:intergenic
CAGAGTTTCAATCTTGAGAT+GGG 0.483383 2_2:+60155783 None:intergenic
GGGAAAATAGAGAAGGAAGA+AGG 0.485572 2_2:-60155428 Msa0261640:three_prime_UTR
TTCAGCAATGCTGGTAATGC+TGG 0.487409 2_2:-60156698 Msa0261640:CDS
TTTGCAGGGAATTACATTGA+AGG 0.494365 2_2:-60156315 Msa0261640:CDS
CACAACTTGGTTGTTGATGG+TGG 0.495208 2_2:-60156230 Msa0261640:CDS
ACAGAGTTTCAATCTTGAGA+TGG 0.500305 2_2:+60155782 None:intergenic
ACTAGAGAATGGGAGAAGGA+AGG 0.500853 2_2:-60155455 Msa0261640:three_prime_UTR
AGAGGTGTAGCAACAGCATA+AGG 0.501265 2_2:+60156401 None:intergenic
GATTAAACTTAGCAAGCAAA+AGG 0.503429 2_2:-60157136 None:intergenic
GGTAAGGTAGCTATAGTGAC+TGG 0.506112 2_2:-60156938 Msa0261640:CDS
CTTGATTATGCTAATTTGCA+GGG 0.512554 2_2:-60156329 Msa0261640:CDS
AGGTCAACATGCAGTGAGCT+TGG 0.516394 2_2:-60156455 Msa0261640:CDS
ACCTCTTGCATGTAGAGCAC+TGG 0.521993 2_2:-60156384 Msa0261640:CDS
AGAGTGAATGATAGAGAAAG+CGG 0.526417 2_2:+60156142 None:intergenic
CAAGCATGCTGCACGTGTAA+TGG 0.527529 2_2:-60156591 Msa0261640:CDS
TTCCTCCTGTGAATAGATCT+CGG 0.528397 2_2:-60155932 Msa0261640:three_prime_UTR
TTGTCATTTAAATAAATGAA+GGG 0.528806 2_2:+60155909 None:intergenic
TAAAGAAGAATGGGATAGTG+AGG 0.534204 2_2:-60155363 Msa0261640:three_prime_UTR
ATCATGGTTGAAATTAGCAT+TGG 0.534963 2_2:+60155606 None:intergenic
CCTGCAAATTAGCATAATCA+AGG 0.541425 2_2:+60156330 None:intergenic
GATAGTGAGGGATGAGTGAA+AGG 0.545912 2_2:-60155350 Msa0261640:three_prime_UTR
ACCAACAAAACCAGCTACAC+TGG 0.550665 2_2:+60156532 None:intergenic
ACCATGGCTGTGAACGTTCG+TGG 0.565494 2_2:-60156629 Msa0261640:CDS
AGGAAAAGGGAAAATAGAGA+AGG 0.567078 2_2:-60155435 Msa0261640:three_prime_UTR
CCGAGTGAAGTCGAAGCTGT+TGG 0.568288 2_2:-60156353 Msa0261640:CDS
TTCTTATAAAATAAGGGGAG+AGG 0.573343 2_2:-60155983 Msa0261640:three_prime_UTR
TTATGTTAGTGGACACAACT+TGG 0.574606 2_2:-60156243 Msa0261640:CDS
ACCACGAACGTTCACAGCCA+TGG 0.577592 2_2:+60156628 None:intergenic
AGAAGCAGTAAAGATATTCG+TGG 0.578218 2_2:-60156894 Msa0261640:CDS
GTAAGGTAGCTATAGTGACT+GGG 0.582532 2_2:-60156937 Msa0261640:CDS
AACACTTCTTATAAAATAAG+GGG 0.583472 2_2:-60155988 Msa0261640:three_prime_UTR
AAGGGCCGAGATCTATTCAC+AGG 0.591917 2_2:+60155927 None:intergenic
TTTAACTAGAGAATGGGAGA+AGG 0.592306 2_2:-60155459 Msa0261640:three_prime_UTR
ATGAAAGGCTGAGATTGCAC+AGG 0.602699 2_2:-60155266 Msa0261640:three_prime_UTR
AGGACATGTGAACCCGTGAA+AGG 0.602781 2_2:-60155408 Msa0261640:three_prime_UTR
AAGGTTCATAACTCAAAGCA+AGG 0.606203 2_2:-60157117 Msa0261640:five_prime_UTR
TCCAGTGCTCTACATGCAAG+AGG 0.608317 2_2:+60156383 None:intergenic
ATAGTGACTGGGGCAGCAAG+CGG 0.610587 2_2:-60156926 Msa0261640:CDS
CATCTCTCAGGTCACAGTGG+TGG 0.620723 2_2:+60156783 None:intergenic
GGAAATTGTTGCTTTCACGT+TGG 0.626510 2_2:-60156747 Msa0261640:CDS
TGCATGTAGAGCACTGGACA+TGG 0.628198 2_2:-60156378 Msa0261640:CDS
CCAACAGCTTCGACTTCACT+CGG 0.628321 2_2:+60156353 None:intergenic
AATCATCAATGTCAAAACAA+AGG 0.629421 2_2:-60157083 Msa0261640:intron
GCATGCTGCACGTGTAATGG+TGG 0.633944 2_2:-60156588 Msa0261640:CDS
AAAGAAGAATGGGATAGTGA+GGG 0.635120 2_2:-60155362 Msa0261640:three_prime_UTR
GAGAGATGAGAAACAAGTTG+AGG 0.635217 2_2:-60156768 Msa0261640:CDS
GGTTGAAATTAGCATTGGTG+TGG 0.645926 2_2:+60155611 None:intergenic
GATTATACAGCATCAAAACA+TGG 0.649473 2_2:-60156494 Msa0261640:CDS
GGCCGAGATCTATTCACAGG+AGG 0.665372 2_2:+60155930 None:intergenic
TCTCATCTCTCAGGTCACAG+TGG 0.665905 2_2:+60156780 None:intergenic
AAATGAATTTGACAACACCA+TGG 0.674818 2_2:-60156645 Msa0261640:CDS
TAAGGTAGCTATAGTGACTG+GGG 0.695873 2_2:-60156936 Msa0261640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTATTTTATGATCAT+AGG - chr2_2:60155270-60155289 Msa0261640:three_prime_UTR 10.0%
!! ATCAGATTAATAAAAAATAA+GGG + chr2_2:60156678-60156697 None:intergenic 10.0%
!! AATCAGATTAATAAAAAATA+AGG + chr2_2:60156679-60156698 None:intergenic 10.0%
!!! TTAATTTGAATAATGATAAA+TGG - chr2_2:60156728-60156747 Msa0261640:CDS 10.0%
!!! ATATTTTATTTTATGATCAT+AGG - chr2_2:60155270-60155289 Msa0261640:three_prime_UTR 10.0%
!! ATCAGATTAATAAAAAATAA+GGG + chr2_2:60156678-60156697 None:intergenic 10.0%
!! AATCAGATTAATAAAAAATA+AGG + chr2_2:60156679-60156698 None:intergenic 10.0%
!!! TTAATTTGAATAATGATAAA+TGG - chr2_2:60156728-60156747 Msa0261640:CDS 10.0%
!!! AGTTATCATTTTTTAATACT+AGG + chr2_2:60156058-60156077 None:intergenic 15.0%
!!! TTAATATTTTTTCTCTTATG+CGG + chr2_2:60156202-60156221 None:intergenic 15.0%
!! ATAACACTTCTTATAAAATA+AGG - chr2_2:60156247-60156266 Msa0261640:CDS 15.0%
!! TAACACTTCTTATAAAATAA+GGG - chr2_2:60156248-60156267 Msa0261640:CDS 15.0%
!!! TTGTCATTTAAATAAATGAA+GGG + chr2_2:60156331-60156350 None:intergenic 15.0%
!!! TTTGTCATTTAAATAAATGA+AGG + chr2_2:60156332-60156351 None:intergenic 15.0%
!! TTATTTAAATGACAAATCAA+TGG - chr2_2:60156336-60156355 Msa0261640:CDS 15.0%
!!! AGTTATCATTTTTTAATACT+AGG + chr2_2:60156058-60156077 None:intergenic 15.0%
!!! TTAATATTTTTTCTCTTATG+CGG + chr2_2:60156202-60156221 None:intergenic 15.0%
!! ATAACACTTCTTATAAAATA+AGG - chr2_2:60156247-60156266 Msa0261640:CDS 15.0%
!! TAACACTTCTTATAAAATAA+GGG - chr2_2:60156248-60156267 Msa0261640:CDS 15.0%
!!! TTGTCATTTAAATAAATGAA+GGG + chr2_2:60156331-60156350 None:intergenic 15.0%
!!! TTTGTCATTTAAATAAATGA+AGG + chr2_2:60156332-60156351 None:intergenic 15.0%
!! TTATTTAAATGACAAATCAA+TGG - chr2_2:60156336-60156355 Msa0261640:CDS 15.0%
!!! TTTATTTTATGATCATAGGT+TGG - chr2_2:60155274-60155293 Msa0261640:three_prime_UTR 20.0%
!! AACACTTCTTATAAAATAAG+GGG - chr2_2:60156249-60156268 Msa0261640:CDS 20.0%
!!! TTTTTTTAGGGAAAATTGTA+GGG + chr2_2:60156566-60156585 None:intergenic 20.0%
!!! TTTTTTTTAGGGAAAATTGT+AGG + chr2_2:60156567-60156586 None:intergenic 20.0%
!! AACTGAAATTTAACATATCA+TGG + chr2_2:60156650-60156669 None:intergenic 20.0%
!! ATGATATGTTAAATTTCAGT+TGG - chr2_2:60156649-60156668 Msa0261640:CDS 20.0%
!!! TTTCTTGTTGATTTGAAATT+AGG - chr2_2:60156705-60156724 Msa0261640:CDS 20.0%
!!! TTTTCTGATATTGTTTAAGA+TGG + chr2_2:60156758-60156777 None:intergenic 20.0%
!! AGAAAATTTAACTAGAGAAT+GGG - chr2_2:60156772-60156791 Msa0261640:CDS 20.0%
!!! TCATTTTTTATAAAGACAGA+TGG + chr2_2:60156955-60156974 None:intergenic 20.0%
!! CTGTCTTTATAAAAAATGAA+AGG - chr2_2:60156956-60156975 Msa0261640:intron 20.0%
!!! TTTATTTTATGATCATAGGT+TGG - chr2_2:60155274-60155293 Msa0261640:three_prime_UTR 20.0%
!! AACACTTCTTATAAAATAAG+GGG - chr2_2:60156249-60156268 Msa0261640:CDS 20.0%
!!! TTTTTTTAGGGAAAATTGTA+GGG + chr2_2:60156566-60156585 None:intergenic 20.0%
!!! TTTTTTTTAGGGAAAATTGT+AGG + chr2_2:60156567-60156586 None:intergenic 20.0%
!! AACTGAAATTTAACATATCA+TGG + chr2_2:60156650-60156669 None:intergenic 20.0%
!! ATGATATGTTAAATTTCAGT+TGG - chr2_2:60156649-60156668 Msa0261640:CDS 20.0%
!!! TTTCTTGTTGATTTGAAATT+AGG - chr2_2:60156705-60156724 Msa0261640:CDS 20.0%
!!! TTTTCTGATATTGTTTAAGA+TGG + chr2_2:60156758-60156777 None:intergenic 20.0%
!! AGAAAATTTAACTAGAGAAT+GGG - chr2_2:60156772-60156791 Msa0261640:CDS 20.0%
!!! TCATTTTTTATAAAGACAGA+TGG + chr2_2:60156955-60156974 None:intergenic 20.0%
!! CTGTCTTTATAAAAAATGAA+AGG - chr2_2:60156956-60156975 Msa0261640:intron 20.0%
! AATCATCAATGTCAAAACAA+AGG - chr2_2:60155154-60155173 Msa0261640:three_prime_UTR 25.0%
! ATCATGTTGCAAATTCAATA+GGG - chr2_2:60155414-60155433 Msa0261640:three_prime_UTR 25.0%
! AATTCTAGTATGCTAGATAA+AGG + chr2_2:60155566-60155585 None:intergenic 25.0%
! TTACAGAGTTTCAAATTGTA+GGG + chr2_2:60156597-60156616 None:intergenic 25.0%
! TTTACAGAGTTTCAAATTGT+AGG + chr2_2:60156598-60156617 None:intergenic 25.0%
! CAGAAAATTTAACTAGAGAA+TGG - chr2_2:60156771-60156790 Msa0261640:CDS 25.0%
!! AAATGGTTTGTAAAGAAGAA+TGG - chr2_2:60156864-60156883 Msa0261640:CDS 25.0%
!! AATGGTTTGTAAAGAAGAAT+GGG - chr2_2:60156865-60156884 Msa0261640:CDS 25.0%
! GAGACAAAATCTGATTTAAT+TGG - chr2_2:60156913-60156932 Msa0261640:CDS 25.0%
!!! TCTGATTTAATTGGTTTTAC+TGG - chr2_2:60156922-60156941 Msa0261640:CDS 25.0%
!! TTTTTATAAAGACAGATGGA+CGG + chr2_2:60156951-60156970 None:intergenic 25.0%
! AAACTGTAGAAAAACTAAAC+AGG - chr2_2:60156997-60157016 Msa0261640:intron 25.0%
!!! AACTCACATAGCTTATTTTA+GGG + chr2_2:60157035-60157054 None:intergenic 25.0%
! AATCATCAATGTCAAAACAA+AGG - chr2_2:60155154-60155173 Msa0261640:three_prime_UTR 25.0%
! ATCATGTTGCAAATTCAATA+GGG - chr2_2:60155414-60155433 Msa0261640:three_prime_UTR 25.0%
! AATTCTAGTATGCTAGATAA+AGG + chr2_2:60155566-60155585 None:intergenic 25.0%
! TTACAGAGTTTCAAATTGTA+GGG + chr2_2:60156597-60156616 None:intergenic 25.0%
! TTTACAGAGTTTCAAATTGT+AGG + chr2_2:60156598-60156617 None:intergenic 25.0%
! CAGAAAATTTAACTAGAGAA+TGG - chr2_2:60156771-60156790 Msa0261640:CDS 25.0%
!! AAATGGTTTGTAAAGAAGAA+TGG - chr2_2:60156864-60156883 Msa0261640:CDS 25.0%
!! AATGGTTTGTAAAGAAGAAT+GGG - chr2_2:60156865-60156884 Msa0261640:CDS 25.0%
! GAGACAAAATCTGATTTAAT+TGG - chr2_2:60156913-60156932 Msa0261640:CDS 25.0%
!!! TCTGATTTAATTGGTTTTAC+TGG - chr2_2:60156922-60156941 Msa0261640:CDS 25.0%
!! TTTTTATAAAGACAGATGGA+CGG + chr2_2:60156951-60156970 None:intergenic 25.0%
! AAACTGTAGAAAAACTAAAC+AGG - chr2_2:60156997-60157016 Msa0261640:intron 25.0%
!!! AACTCACATAGCTTATTTTA+GGG + chr2_2:60157035-60157054 None:intergenic 25.0%
TGTTATAAGCCAATAGAAAC+TGG + chr2_2:60155236-60155255 None:intergenic 30.0%
!! ATTTTATGATCATAGGTTGG+AGG - chr2_2:60155277-60155296 Msa0261640:three_prime_UTR 30.0%
!! TTTTATGATCATAGGTTGGA+GGG - chr2_2:60155278-60155297 Msa0261640:three_prime_UTR 30.0%
GTAAAGATATTCGTGGAAAA+TGG - chr2_2:60155350-60155369 Msa0261640:three_prime_UTR 30.0%
AGCAGATATCAATGATGAAT+TGG - chr2_2:60155388-60155407 Msa0261640:three_prime_UTR 30.0%
GCAGATATCAATGATGAATT+GGG - chr2_2:60155389-60155408 Msa0261640:three_prime_UTR 30.0%
CATCATGTTGCAAATTCAAT+AGG - chr2_2:60155413-60155432 Msa0261640:three_prime_UTR 30.0%
!! TGGTGTTGTCAAATTCATTT+AGG + chr2_2:60155592-60155611 None:intergenic 30.0%
AAATGAATTTGACAACACCA+TGG - chr2_2:60155592-60155611 Msa0261640:three_prime_UTR 30.0%
GATTATACAGCATCAAAACA+TGG - chr2_2:60155743-60155762 Msa0261640:three_prime_UTR 30.0%
! CTTGATTATGCTAATTTGCA+GGG - chr2_2:60155908-60155927 Msa0261640:three_prime_UTR 30.0%
TTCTTATAAAATAAGGGGAG+AGG - chr2_2:60156254-60156273 Msa0261640:CDS 30.0%
!! TTCTCTATTTCATGTCGTTT+TGG - chr2_2:60156391-60156410 Msa0261640:CDS 30.0%
GTGACTTTGTGAGAAAAAAA+GGG + chr2_2:60156431-60156450 None:intergenic 30.0%
TGTGACTTTGTGAGAAAAAA+AGG + chr2_2:60156432-60156451 None:intergenic 30.0%
!!! AGGGATGCATCTTTTTTTTA+GGG + chr2_2:60156578-60156597 None:intergenic 30.0%
!!! TAGGGATGCATCTTTTTTTT+AGG + chr2_2:60156579-60156598 None:intergenic 30.0%
ATCATGGTTGAAATTAGCAT+TGG + chr2_2:60156634-60156653 None:intergenic 30.0%
AAAGGAATGAAGCAGAAAAA+TGG - chr2_2:60156847-60156866 Msa0261640:CDS 30.0%
!!! ACATAGCTTATTTTAGGGAA+TGG + chr2_2:60157030-60157049 None:intergenic 30.0%
!! CAACTCACATAGCTTATTTT+AGG + chr2_2:60157036-60157055 None:intergenic 30.0%
CTAAAATAAGCTATGTGAGT+TGG - chr2_2:60157034-60157053 Msa0261640:intron 30.0%
!!! AAACGTTCTTCTTTTTTGAG+AGG - chr2_2:60157071-60157090 Msa0261640:intron 30.0%
TGTTATAAGCCAATAGAAAC+TGG + chr2_2:60155236-60155255 None:intergenic 30.0%
!! ATTTTATGATCATAGGTTGG+AGG - chr2_2:60155277-60155296 Msa0261640:three_prime_UTR 30.0%
!! TTTTATGATCATAGGTTGGA+GGG - chr2_2:60155278-60155297 Msa0261640:three_prime_UTR 30.0%
GTAAAGATATTCGTGGAAAA+TGG - chr2_2:60155350-60155369 Msa0261640:three_prime_UTR 30.0%
AGCAGATATCAATGATGAAT+TGG - chr2_2:60155388-60155407 Msa0261640:three_prime_UTR 30.0%
GCAGATATCAATGATGAATT+GGG - chr2_2:60155389-60155408 Msa0261640:three_prime_UTR 30.0%
CATCATGTTGCAAATTCAAT+AGG - chr2_2:60155413-60155432 Msa0261640:three_prime_UTR 30.0%
!! TGGTGTTGTCAAATTCATTT+AGG + chr2_2:60155592-60155611 None:intergenic 30.0%
AAATGAATTTGACAACACCA+TGG - chr2_2:60155592-60155611 Msa0261640:three_prime_UTR 30.0%
GATTATACAGCATCAAAACA+TGG - chr2_2:60155743-60155762 Msa0261640:three_prime_UTR 30.0%
! CTTGATTATGCTAATTTGCA+GGG - chr2_2:60155908-60155927 Msa0261640:three_prime_UTR 30.0%
TTCTTATAAAATAAGGGGAG+AGG - chr2_2:60156254-60156273 Msa0261640:CDS 30.0%
!! TTCTCTATTTCATGTCGTTT+TGG - chr2_2:60156391-60156410 Msa0261640:CDS 30.0%
GTGACTTTGTGAGAAAAAAA+GGG + chr2_2:60156431-60156450 None:intergenic 30.0%
TGTGACTTTGTGAGAAAAAA+AGG + chr2_2:60156432-60156451 None:intergenic 30.0%
!!! AGGGATGCATCTTTTTTTTA+GGG + chr2_2:60156578-60156597 None:intergenic 30.0%
!!! TAGGGATGCATCTTTTTTTT+AGG + chr2_2:60156579-60156598 None:intergenic 30.0%
ATCATGGTTGAAATTAGCAT+TGG + chr2_2:60156634-60156653 None:intergenic 30.0%
AAAGGAATGAAGCAGAAAAA+TGG - chr2_2:60156847-60156866 Msa0261640:CDS 30.0%
!!! ACATAGCTTATTTTAGGGAA+TGG + chr2_2:60157030-60157049 None:intergenic 30.0%
!! CAACTCACATAGCTTATTTT+AGG + chr2_2:60157036-60157055 None:intergenic 30.0%
CTAAAATAAGCTATGTGAGT+TGG - chr2_2:60157034-60157053 Msa0261640:intron 30.0%
!!! AAACGTTCTTCTTTTTTGAG+AGG - chr2_2:60157071-60157090 Msa0261640:intron 30.0%
AAGGTTCATAACTCAAAGCA+AGG - chr2_2:60155120-60155139 Msa0261640:three_prime_UTR 35.0%
AGAAGCAGTAAAGATATTCG+TGG - chr2_2:60155343-60155362 Msa0261640:three_prime_UTR 35.0%
ATTGCTGAACATGATGTCTA+AGG + chr2_2:60155528-60155547 None:intergenic 35.0%
! ATGGTGGAAAGAAAAATACG+TGG - chr2_2:60155665-60155684 Msa0261640:three_prime_UTR 35.0%
!! GCATCAAAACATGGTCTTAT+AGG - chr2_2:60155752-60155771 Msa0261640:three_prime_UTR 35.0%
!! ATGGTCTTATAGGACTTGTT+AGG - chr2_2:60155762-60155781 Msa0261640:three_prime_UTR 35.0%
CCTGCAAATTAGCATAATCA+AGG + chr2_2:60155910-60155929 None:intergenic 35.0%
! CCTTGATTATGCTAATTTGC+AGG - chr2_2:60155907-60155926 Msa0261640:three_prime_UTR 35.0%
TTTGCAGGGAATTACATTGA+AGG - chr2_2:60155922-60155941 Msa0261640:three_prime_UTR 35.0%
GATGAATCTGCTTATGTTAG+TGG - chr2_2:60155983-60156002 Msa0261640:three_prime_UTR 35.0%
! TTATGTTAGTGGACACAACT+TGG - chr2_2:60155994-60156013 Msa0261640:three_prime_UTR 35.0%
AGAGTGAATGATAGAGAAAG+CGG + chr2_2:60156098-60156117 None:intergenic 35.0%
AAACAGGAGAGAAAAAACAC+TGG + chr2_2:60156287-60156306 None:intergenic 35.0%
CAGAGTTTCAATCTTGAGAT+GGG + chr2_2:60156457-60156476 None:intergenic 35.0%
ACAGAGTTTCAATCTTGAGA+TGG + chr2_2:60156458-60156477 None:intergenic 35.0%
TTTAACTAGAGAATGGGAGA+AGG - chr2_2:60156778-60156797 Msa0261640:CDS 35.0%
AGGAAAAGGGAAAATAGAGA+AGG - chr2_2:60156802-60156821 Msa0261640:CDS 35.0%
TTTCTGCTTCATTCCTTTCA+CGG + chr2_2:60156845-60156864 None:intergenic 35.0%
TAAAGAAGAATGGGATAGTG+AGG - chr2_2:60156874-60156893 Msa0261640:CDS 35.0%
AAAGAAGAATGGGATAGTGA+GGG - chr2_2:60156875-60156894 Msa0261640:CDS 35.0%
AAGGTTCATAACTCAAAGCA+AGG - chr2_2:60155120-60155139 Msa0261640:three_prime_UTR 35.0%
AGAAGCAGTAAAGATATTCG+TGG - chr2_2:60155343-60155362 Msa0261640:three_prime_UTR 35.0%
ATTGCTGAACATGATGTCTA+AGG + chr2_2:60155528-60155547 None:intergenic 35.0%
! ATGGTGGAAAGAAAAATACG+TGG - chr2_2:60155665-60155684 Msa0261640:three_prime_UTR 35.0%
!! GCATCAAAACATGGTCTTAT+AGG - chr2_2:60155752-60155771 Msa0261640:three_prime_UTR 35.0%
!! ATGGTCTTATAGGACTTGTT+AGG - chr2_2:60155762-60155781 Msa0261640:three_prime_UTR 35.0%
CCTGCAAATTAGCATAATCA+AGG + chr2_2:60155910-60155929 None:intergenic 35.0%
! CCTTGATTATGCTAATTTGC+AGG - chr2_2:60155907-60155926 Msa0261640:three_prime_UTR 35.0%
TTTGCAGGGAATTACATTGA+AGG - chr2_2:60155922-60155941 Msa0261640:three_prime_UTR 35.0%
GATGAATCTGCTTATGTTAG+TGG - chr2_2:60155983-60156002 Msa0261640:three_prime_UTR 35.0%
! TTATGTTAGTGGACACAACT+TGG - chr2_2:60155994-60156013 Msa0261640:three_prime_UTR 35.0%
AGAGTGAATGATAGAGAAAG+CGG + chr2_2:60156098-60156117 None:intergenic 35.0%
AAACAGGAGAGAAAAAACAC+TGG + chr2_2:60156287-60156306 None:intergenic 35.0%
CAGAGTTTCAATCTTGAGAT+GGG + chr2_2:60156457-60156476 None:intergenic 35.0%
ACAGAGTTTCAATCTTGAGA+TGG + chr2_2:60156458-60156477 None:intergenic 35.0%
TTTAACTAGAGAATGGGAGA+AGG - chr2_2:60156778-60156797 Msa0261640:CDS 35.0%
AGGAAAAGGGAAAATAGAGA+AGG - chr2_2:60156802-60156821 Msa0261640:CDS 35.0%
TTTCTGCTTCATTCCTTTCA+CGG + chr2_2:60156845-60156864 None:intergenic 35.0%
TAAAGAAGAATGGGATAGTG+AGG - chr2_2:60156874-60156893 Msa0261640:CDS 35.0%
AAAGAAGAATGGGATAGTGA+GGG - chr2_2:60156875-60156894 Msa0261640:CDS 35.0%
CACTGTACTAGCTAGAAAGA+GGG + chr2_2:60155209-60155228 None:intergenic 40.0%
ACACTGTACTAGCTAGAAAG+AGG + chr2_2:60155210-60155229 None:intergenic 40.0%
CAGTGTCTACCAGTTTCTAT+TGG - chr2_2:60155224-60155243 Msa0261640:three_prime_UTR 40.0%
GTAAGGTAGCTATAGTGACT+GGG - chr2_2:60155300-60155319 Msa0261640:three_prime_UTR 40.0%
TAAGGTAGCTATAGTGACTG+GGG - chr2_2:60155301-60155320 Msa0261640:three_prime_UTR 40.0%
! CAACTTGTTTCTCATCTCTC+AGG + chr2_2:60155469-60155488 None:intergenic 40.0%
GAGAGATGAGAAACAAGTTG+AGG - chr2_2:60155469-60155488 Msa0261640:three_prime_UTR 40.0%
!! GGAAATTGTTGCTTTCACGT+TGG - chr2_2:60155490-60155509 Msa0261640:three_prime_UTR 40.0%
TACACTGGCAGTGCAAATTA+TGG + chr2_2:60155693-60155712 None:intergenic 40.0%
GTGGCTTCTCAGTCATTAAT+CGG - chr2_2:60156026-60156045 Msa0261640:three_prime_UTR 40.0%
ATGATAGAGAAAGCGGTAGA+AGG + chr2_2:60156091-60156110 None:intergenic 40.0%
ATCTATTCACAGGAGGAAAC+AGG + chr2_2:60156303-60156322 None:intergenic 40.0%
TTCCTCCTGTGAATAGATCT+CGG - chr2_2:60156305-60156324 Msa0261640:CDS 40.0%
GTGAGAAAAAAAGGGAACAC+TGG + chr2_2:60156423-60156442 None:intergenic 40.0%
!! GGTTGAAATTAGCATTGGTG+TGG + chr2_2:60156629-60156648 None:intergenic 40.0%
AATGGGAGAAGGAAGGAAAA+GGG - chr2_2:60156789-60156808 Msa0261640:CDS 40.0%
GGGAAAATAGAGAAGGAAGA+AGG - chr2_2:60156809-60156828 Msa0261640:CDS 40.0%
TTCTGCTTCATTCCTTTCAC+GGG + chr2_2:60156844-60156863 None:intergenic 40.0%
CACTGTACTAGCTAGAAAGA+GGG + chr2_2:60155209-60155228 None:intergenic 40.0%
ACACTGTACTAGCTAGAAAG+AGG + chr2_2:60155210-60155229 None:intergenic 40.0%
CAGTGTCTACCAGTTTCTAT+TGG - chr2_2:60155224-60155243 Msa0261640:three_prime_UTR 40.0%
GTAAGGTAGCTATAGTGACT+GGG - chr2_2:60155300-60155319 Msa0261640:three_prime_UTR 40.0%
TAAGGTAGCTATAGTGACTG+GGG - chr2_2:60155301-60155320 Msa0261640:three_prime_UTR 40.0%
! CAACTTGTTTCTCATCTCTC+AGG + chr2_2:60155469-60155488 None:intergenic 40.0%
GAGAGATGAGAAACAAGTTG+AGG - chr2_2:60155469-60155488 Msa0261640:three_prime_UTR 40.0%
!! GGAAATTGTTGCTTTCACGT+TGG - chr2_2:60155490-60155509 Msa0261640:three_prime_UTR 40.0%
TACACTGGCAGTGCAAATTA+TGG + chr2_2:60155693-60155712 None:intergenic 40.0%
GTGGCTTCTCAGTCATTAAT+CGG - chr2_2:60156026-60156045 Msa0261640:three_prime_UTR 40.0%
ATGATAGAGAAAGCGGTAGA+AGG + chr2_2:60156091-60156110 None:intergenic 40.0%
ATCTATTCACAGGAGGAAAC+AGG + chr2_2:60156303-60156322 None:intergenic 40.0%
TTCCTCCTGTGAATAGATCT+CGG - chr2_2:60156305-60156324 Msa0261640:CDS 40.0%
GTGAGAAAAAAAGGGAACAC+TGG + chr2_2:60156423-60156442 None:intergenic 40.0%
!! GGTTGAAATTAGCATTGGTG+TGG + chr2_2:60156629-60156648 None:intergenic 40.0%
AATGGGAGAAGGAAGGAAAA+GGG - chr2_2:60156789-60156808 Msa0261640:CDS 40.0%
GGGAAAATAGAGAAGGAAGA+AGG - chr2_2:60156809-60156828 Msa0261640:CDS 40.0%
TTCTGCTTCATTCCTTTCAC+GGG + chr2_2:60156844-60156863 None:intergenic 40.0%
! TGATCATAGGTTGGAGGGTA+AGG - chr2_2:60155283-60155302 Msa0261640:three_prime_UTR 45.0%
GGTAAGGTAGCTATAGTGAC+TGG - chr2_2:60155299-60155318 Msa0261640:three_prime_UTR 45.0%
GCTTTCACGTTGGAGAGATA+TGG - chr2_2:60155500-60155519 Msa0261640:three_prime_UTR 45.0%
GACATCATGTTCAGCAATGC+TGG - chr2_2:60155530-60155549 Msa0261640:three_prime_UTR 45.0%
! TTCAGCAATGCTGGTAATGC+TGG - chr2_2:60155539-60155558 Msa0261640:three_prime_UTR 45.0%
ACCAACAAAACCAGCTACAC+TGG + chr2_2:60155708-60155727 None:intergenic 45.0%
!!! AGTGTAGCTGGTTTTGTTGG+TGG - chr2_2:60155707-60155726 Msa0261640:three_prime_UTR 45.0%
! AGAGGTGTAGCAACAGCATA+AGG + chr2_2:60155839-60155858 None:intergenic 45.0%
GGACACAACTTGGTTGTTGA+TGG - chr2_2:60156004-60156023 Msa0261640:three_prime_UTR 45.0%
! CACAACTTGGTTGTTGATGG+TGG - chr2_2:60156007-60156026 Msa0261640:three_prime_UTR 45.0%
GGAGAGAAAAAACACTGGAG+AGG + chr2_2:60156282-60156301 None:intergenic 45.0%
ACTAGAGAATGGGAGAAGGA+AGG - chr2_2:60156782-60156801 Msa0261640:CDS 45.0%
GAATGGGAGAAGGAAGGAAA+AGG - chr2_2:60156788-60156807 Msa0261640:CDS 45.0%
GATAGTGAGGGATGAGTGAA+AGG - chr2_2:60156887-60156906 Msa0261640:CDS 45.0%
ATGAAAGGCTGAGATTGCAC+AGG - chr2_2:60156971-60156990 Msa0261640:intron 45.0%
! TGATCATAGGTTGGAGGGTA+AGG - chr2_2:60155283-60155302 Msa0261640:three_prime_UTR 45.0%
GGTAAGGTAGCTATAGTGAC+TGG - chr2_2:60155299-60155318 Msa0261640:three_prime_UTR 45.0%
GCTTTCACGTTGGAGAGATA+TGG - chr2_2:60155500-60155519 Msa0261640:three_prime_UTR 45.0%
GACATCATGTTCAGCAATGC+TGG - chr2_2:60155530-60155549 Msa0261640:three_prime_UTR 45.0%
! TTCAGCAATGCTGGTAATGC+TGG - chr2_2:60155539-60155558 Msa0261640:three_prime_UTR 45.0%
ACCAACAAAACCAGCTACAC+TGG + chr2_2:60155708-60155727 None:intergenic 45.0%
!!! AGTGTAGCTGGTTTTGTTGG+TGG - chr2_2:60155707-60155726 Msa0261640:three_prime_UTR 45.0%
! AGAGGTGTAGCAACAGCATA+AGG + chr2_2:60155839-60155858 None:intergenic 45.0%
GGACACAACTTGGTTGTTGA+TGG - chr2_2:60156004-60156023 Msa0261640:three_prime_UTR 45.0%
! CACAACTTGGTTGTTGATGG+TGG - chr2_2:60156007-60156026 Msa0261640:three_prime_UTR 45.0%
GGAGAGAAAAAACACTGGAG+AGG + chr2_2:60156282-60156301 None:intergenic 45.0%
ACTAGAGAATGGGAGAAGGA+AGG - chr2_2:60156782-60156801 Msa0261640:CDS 45.0%
GAATGGGAGAAGGAAGGAAA+AGG - chr2_2:60156788-60156807 Msa0261640:CDS 45.0%
GATAGTGAGGGATGAGTGAA+AGG - chr2_2:60156887-60156906 Msa0261640:CDS 45.0%
ATGAAAGGCTGAGATTGCAC+AGG - chr2_2:60156971-60156990 Msa0261640:intron 45.0%
!! GTATATTATATTTATAATAT+TGG - chr2_2:60157125-60157144 Msa0261640:five_prime_UTR 5.0%
!! GTATATTATATTTATAATAT+TGG - chr2_2:60157125-60157144 Msa0261640:five_prime_UTR 5.0%
TCTCATCTCTCAGGTCACAG+TGG + chr2_2:60155460-60155479 None:intergenic 50.0%
CAAGCATGCTGCACGTGTAA+TGG - chr2_2:60155646-60155665 Msa0261640:three_prime_UTR 50.0%
ATTTGCACTGCCAGTGTAGC+TGG - chr2_2:60155695-60155714 Msa0261640:three_prime_UTR 50.0%
!!! GCCAGTGTAGCTGGTTTTGT+TGG - chr2_2:60155704-60155723 Msa0261640:three_prime_UTR 50.0%
!!! GGTTTTGTTGGTGGATGTGC+AGG - chr2_2:60155716-60155735 Msa0261640:three_prime_UTR 50.0%
AGGTCAACATGCAGTGAGCT+TGG - chr2_2:60155782-60155801 Msa0261640:three_prime_UTR 50.0%
TGCAGTGAGCTTGGAGCTTA+TGG - chr2_2:60155791-60155810 Msa0261640:three_prime_UTR 50.0%
ACCTCTTGCATGTAGAGCAC+TGG - chr2_2:60155853-60155872 Msa0261640:three_prime_UTR 50.0%
TCCAGTGCTCTACATGCAAG+AGG + chr2_2:60155857-60155876 None:intergenic 50.0%
!! TGCATGTAGAGCACTGGACA+TGG - chr2_2:60155859-60155878 Msa0261640:three_prime_UTR 50.0%
CCAACAGCTTCGACTTCACT+CGG + chr2_2:60155887-60155906 None:intergenic 50.0%
AAGGGCCGAGATCTATTCAC+AGG + chr2_2:60156313-60156332 None:intergenic 50.0%
AGGACATGTGAACCCGTGAA+AGG - chr2_2:60156829-60156848 Msa0261640:CDS 50.0%
TCTCATCTCTCAGGTCACAG+TGG + chr2_2:60155460-60155479 None:intergenic 50.0%
CAAGCATGCTGCACGTGTAA+TGG - chr2_2:60155646-60155665 Msa0261640:three_prime_UTR 50.0%
ATTTGCACTGCCAGTGTAGC+TGG - chr2_2:60155695-60155714 Msa0261640:three_prime_UTR 50.0%
!!! GCCAGTGTAGCTGGTTTTGT+TGG - chr2_2:60155704-60155723 Msa0261640:three_prime_UTR 50.0%
!!! GGTTTTGTTGGTGGATGTGC+AGG - chr2_2:60155716-60155735 Msa0261640:three_prime_UTR 50.0%
AGGTCAACATGCAGTGAGCT+TGG - chr2_2:60155782-60155801 Msa0261640:three_prime_UTR 50.0%
TGCAGTGAGCTTGGAGCTTA+TGG - chr2_2:60155791-60155810 Msa0261640:three_prime_UTR 50.0%
ACCTCTTGCATGTAGAGCAC+TGG - chr2_2:60155853-60155872 Msa0261640:three_prime_UTR 50.0%
TCCAGTGCTCTACATGCAAG+AGG + chr2_2:60155857-60155876 None:intergenic 50.0%
!! TGCATGTAGAGCACTGGACA+TGG - chr2_2:60155859-60155878 Msa0261640:three_prime_UTR 50.0%
CCAACAGCTTCGACTTCACT+CGG + chr2_2:60155887-60155906 None:intergenic 50.0%
AAGGGCCGAGATCTATTCAC+AGG + chr2_2:60156313-60156332 None:intergenic 50.0%
AGGACATGTGAACCCGTGAA+AGG - chr2_2:60156829-60156848 Msa0261640:CDS 50.0%
ATAGTGACTGGGGCAGCAAG+CGG - chr2_2:60155311-60155330 Msa0261640:three_prime_UTR 55.0%
CATCTCTCAGGTCACAGTGG+TGG + chr2_2:60155457-60155476 None:intergenic 55.0%
ACCATGGCTGTGAACGTTCG+TGG - chr2_2:60155608-60155627 Msa0261640:three_prime_UTR 55.0%
ACCACGAACGTTCACAGCCA+TGG + chr2_2:60155612-60155631 None:intergenic 55.0%
GCATGCTGCACGTGTAATGG+TGG - chr2_2:60155649-60155668 Msa0261640:three_prime_UTR 55.0%
CCGAGTGAAGTCGAAGCTGT+TGG - chr2_2:60155884-60155903 Msa0261640:three_prime_UTR 55.0%
GGCCGAGATCTATTCACAGG+AGG + chr2_2:60156310-60156329 None:intergenic 55.0%
ATAGTGACTGGGGCAGCAAG+CGG - chr2_2:60155311-60155330 Msa0261640:three_prime_UTR 55.0%
CATCTCTCAGGTCACAGTGG+TGG + chr2_2:60155457-60155476 None:intergenic 55.0%
ACCATGGCTGTGAACGTTCG+TGG - chr2_2:60155608-60155627 Msa0261640:three_prime_UTR 55.0%
ACCACGAACGTTCACAGCCA+TGG + chr2_2:60155612-60155631 None:intergenic 55.0%
GCATGCTGCACGTGTAATGG+TGG - chr2_2:60155649-60155668 Msa0261640:three_prime_UTR 55.0%
CCGAGTGAAGTCGAAGCTGT+TGG - chr2_2:60155884-60155903 Msa0261640:three_prime_UTR 55.0%
GGCCGAGATCTATTCACAGG+AGG + chr2_2:60156310-60156329 None:intergenic 55.0%
ACTGGGGCAGCAAGCGGAAT+AGG - chr2_2:60155317-60155336 Msa0261640:three_prime_UTR 60.0%
ACTGGGGCAGCAAGCGGAAT+AGG - chr2_2:60155317-60155336 Msa0261640:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 60155111 60157148 60155111 ID=Msa0261640;Name=Msa0261640
chr2_2 mRNA 60155111 60157148 60155111 ID=Msa0261640-mRNA-1;Parent=Msa0261640;Name=Msa0261640-mRNA-1;_AED=0.01;_eAED=0.01;_QI=51|1|1|1|1|1|2|1076|264
chr2_2 exon 60155111 60156967 60155111 ID=Msa0261640-mRNA-1:exon:18498;Parent=Msa0261640-mRNA-1
chr2_2 exon 60157084 60157148 60157084 ID=Msa0261640-mRNA-1:exon:18497;Parent=Msa0261640-mRNA-1
chr2_2 five_prime_UTR 60157098 60157148 60157098 ID=Msa0261640-mRNA-1:five_prime_utr;Parent=Msa0261640-mRNA-1
chr2_2 CDS 60157084 60157097 60157084 ID=Msa0261640-mRNA-1:cds;Parent=Msa0261640-mRNA-1
chr2_2 CDS 60156187 60156967 60156187 ID=Msa0261640-mRNA-1:cds;Parent=Msa0261640-mRNA-1
chr2_2 three_prime_UTR 60155111 60156186 60155111 ID=Msa0261640-mRNA-1:three_prime_utr;Parent=Msa0261640-mRNA-1
Gene Sequence

>Msa0261640

ATGTCAAAACAAAGGTTGGAGGGTAAGGTAGCTATAGTGACTGGGGCAGCAAGCGGAATAGGAGCAGAAGCAGTAAAGATATTCGTGGAAAATGGAGCATTTGTAGTCATAGCAGATATCAATGATGAATTGGGTCATCATGTTGCAAATTCAATAGGGTTAGACAAAGTGAGTTACCACCACTGTGACCTGAGAGATGAGAAACAAGTTGAGGAAATTGTTGCTTTCACGTTGGAGAGATATGGTACCTTAGACATCATGTTCAGCAATGCTGGTAATGCTGGTCCTTTATCTAGCATACTAGAATTTGACCTAAATGAATTTGACAACACCATGGCTGTGAACGTTCGTGGTGCAGCTGCAACTATCAAGCATGCTGCACGTGTAATGGTGGAAAGAAAAATACGTGGATCCATAATTTGCACTGCCAGTGTAGCTGGTTTTGTTGGTGGATGTGCAGGACATGATTATACAGCATCAAAACATGGTCTTATAGGACTTGTTAGGTCAACATGCAGTGAGCTTGGAGCTTATGGAATCAGAGTCAATTCAATCTCACCTTATGCTGTTGCTACACCTCTTGCATGTAGAGCACTGGACATGGTGCCGAGTGAAGTCGAAGCTGTTGGCCTTGATTATGCTAATTTGCAGGGAATTACATTGAAGGCTGCTCATATTGCAGAGACTGCTTTGTTTCTTGCATCTGATGAATCTGCTTATGTTAGTGGACACAACTTGGTTGTTGATGGTGGCTTCTCAGTCATTAATCGGTGTCTTCCTAGTATTAAAAAATGA
Protein sequence

>Msa0261640

MSKQRLEGKVAIVTGAASGIGAEAVKIFVENGAFVVIADINDELGHHVANSIGLDKVSYHHCDLRDEKQVEEIVAFTLERYGTLDIMFSNAGNAGPLSSILEFDLNEFDNTMAVNVRGAAATIKHAARVMVERKIRGSIICTASVAGFVGGCAGHDYTASKHGLIGLVRSTCSELGAYGIRVNSISPYAVATPLACRALDMVPSEVEAVGLDYANLQGITLKAAHIAETALFLASDESAYVSGHNLVVDGGFSVINRCLPSIKK*