AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0271840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0271840 MtrunA17_Chr2g0315311 81.148 122 18 2 1 117 1 122 4.89e-55 167
Msa0271840 MtrunA17_Chr2g0315301 57.746 142 20 4 1 114 1 130 1.99e-40 131
Msa0271840 MtrunA17_Chr2g0315321 67.544 114 24 3 1 114 1 101 4.82e-33 112
Msa0271840 MtrunA17_Chr2g0315291 65.789 114 28 1 1 114 1 103 1.75e-26 95.5
Msa0271840 MtrunA17_Chr2g0315331 60.976 82 22 2 1 72 1 82 6.59e-24 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTTGTGCATGATTATT+TGG 0.069508 2_3:-3351497 Msa0271840:five_prime_UTR
TATCATAAGGACATTGTTTA+AGG 0.172598 2_3:+3349904 None:intergenic
TTCTTTGTGCATGATTATTT+GGG 0.272311 2_3:-3351496 Msa0271840:five_prime_UTR
AGCACAGGAAGCGGAGGATT+CGG 0.330833 2_3:-3350234 Msa0271840:CDS
AGCACAGGAAGCGGAGGATT+GGG 0.335111 2_3:-3350129 Msa0271840:CDS
GCACAGGAAGCGGAGGATTC+GGG 0.352203 2_3:-3350233 Msa0271840:CDS
TAGCACAGGAAGCGGAGGAT+TGG 0.389817 2_3:-3350130 Msa0271840:CDS
TTAAACAAACACACTAACTT+TGG 0.404636 2_3:-3351535 Msa0271840:five_prime_UTR
GGGTGGGCAGCACTGGCATG+TGG 0.407335 2_3:+3351408 None:intergenic
TATTTGAGGGTGGGCAGCAC+TGG 0.427483 2_3:+3351401 None:intergenic
ATAATTTGTAATATTTCAAA+TGG 0.428374 2_3:-3351453 Msa0271840:exon
CATAACTATACAATGTCTTA+AGG 0.435385 2_3:+3349987 None:intergenic
ATCAGAGGTTTATGCGTTTG+CGG 0.444972 2_3:+3350099 None:intergenic
TAACCATCATTTGTCGATGT+TGG 0.450141 2_3:+3350204 None:intergenic
TTTCAAATGGCTCTTGCAGC+AGG 0.451956 2_3:-3351440 Msa0271840:exon
GGTGGGCAGCACTGGCATGT+GGG 0.487384 2_3:+3351409 None:intergenic
CCGCTTCCTGTGCTACCCGT+TGG 0.493161 2_3:+3350138 None:intergenic
TTTGCTAACAAAGTATCATA+AGG 0.496127 2_3:+3349891 None:intergenic
CCGCTTCCTGTGCTACCCCA+TGG 0.508962 2_3:+3350243 None:intergenic
GATCCAACATCGACAAATGA+TGG 0.518991 2_3:-3350207 Msa0271840:CDS
GCACAGGAAGCGGAGGATTG+GGG 0.533668 2_3:-3350128 Msa0271840:CDS
ATGAAACCATGGGGTAGCAC+AGG 0.548974 2_3:-3350249 Msa0271840:CDS
CCATGGGGTAGCACAGGAAG+CGG 0.550791 2_3:-3350243 Msa0271840:CDS
AGTTTATGCAATGAAACCAT+GGG 0.551033 2_3:-3350259 Msa0271840:CDS
AAGTTTATGCAATGAAACCA+TGG 0.558083 2_3:-3350260 Msa0271840:CDS
CCAACGGGTAGCACAGGAAG+CGG 0.577109 2_3:-3350138 Msa0271840:CDS
TTTAGACGCATATTTGAGGG+TGG 0.587811 2_3:+3351391 None:intergenic
TTAGACGCATATTTGAGGGT+GGG 0.609382 2_3:+3351392 None:intergenic
TCTTTGTGCATGATTATTTG+GGG 0.629539 2_3:-3351495 Msa0271840:five_prime_UTR
TGGGGTAGCACAGGAAGCGG+AGG 0.630133 2_3:-3350240 Msa0271840:CDS
ACGGGTAGCACAGGAAGCGG+AGG 0.642552 2_3:-3350135 Msa0271840:CDS
GATGAAGATCAACTTGACAA+TGG 0.661313 2_3:-3350057 Msa0271840:CDS
TGACAATGGCAATGAGTGAG+TGG 0.667796 2_3:-3350043 Msa0271840:exon
AGGAAGCGGAGGATTCGGGA+AGG 0.688019 2_3:-3350229 Msa0271840:CDS
GTTTATGCAATGAAACCATG+GGG 0.718021 2_3:-3350258 Msa0271840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATAAATTTAAATTTTT+TGG - chr2_3:3350306-3350325 Msa0271840:intron 0.0%
!!! TTTATAAATTTAAATTTTTT+GGG - chr2_3:3350307-3350326 Msa0271840:intron 0.0%
!!! TTTTATAAATTTAAATTTTT+TGG - chr2_3:3350306-3350325 Msa0271840:intron 0.0%
!!! TTTATAAATTTAAATTTTTT+GGG - chr2_3:3350307-3350326 Msa0271840:intron 0.0%
!!! ATAATTTGTAATATTTCAAA+TGG - chr2_3:3349955-3349974 Msa0271840:three_prime_UTR 10.0%
!!! TTTAAGGAATTTAAAATTTT+AGG - chr2_3:3350189-3350208 Msa0271840:CDS 10.0%
!! CAAATTTCAAAAAATTTAAA+AGG - chr2_3:3350364-3350383 Msa0271840:intron 10.0%
!!! TTATAATTTTGGAAATTTAT+TGG - chr2_3:3350392-3350411 Msa0271840:intron 10.0%
!!! TATAATTTTGGAAATTTATT+GGG - chr2_3:3350393-3350412 Msa0271840:intron 10.0%
!!! ATTTTAAATTCTTAACTTTT+AGG - chr2_3:3350485-3350504 Msa0271840:intron 10.0%
!!! ATAATTTGTAATATTTCAAA+TGG - chr2_3:3349955-3349974 Msa0271840:three_prime_UTR 10.0%
!!! TTTAAGGAATTTAAAATTTT+AGG - chr2_3:3350189-3350208 Msa0271840:CDS 10.0%
!! CAAATTTCAAAAAATTTAAA+AGG - chr2_3:3350364-3350383 Msa0271840:intron 10.0%
!!! TTATAATTTTGGAAATTTAT+TGG - chr2_3:3350392-3350411 Msa0271840:intron 10.0%
!!! TATAATTTTGGAAATTTATT+GGG - chr2_3:3350393-3350412 Msa0271840:intron 10.0%
!!! ATTTTAAATTCTTAACTTTT+AGG - chr2_3:3350485-3350504 Msa0271840:intron 10.0%
!! ATATCAAATATATACTTACA+TGG + chr2_3:3350059-3350078 None:intergenic 15.0%
!!! TATTTCTAAATATTCTTGTT+TGG - chr2_3:3350241-3350260 Msa0271840:CDS 15.0%
!!! AATTCATTTTCATCATTTTT+AGG - chr2_3:3350279-3350298 Msa0271840:CDS 15.0%
!!! AAAAGGTCAATTTATAATTT+TGG - chr2_3:3350381-3350400 Msa0271840:intron 15.0%
!!! ATAATTTTGGAAATTTATTG+GGG - chr2_3:3350394-3350413 Msa0271840:intron 15.0%
!!! TGAAAATTTTGGAAAATTTA+AGG - chr2_3:3350423-3350442 Msa0271840:intron 15.0%
!!! AATTAGTTTCTTAATTAAAC+TGG + chr2_3:3351012-3351031 None:intergenic 15.0%
!! ATAGATTTATAATCATTCAT+TGG - chr2_3:3351040-3351059 Msa0271840:intron 15.0%
!! ATATCAAATATATACTTACA+TGG + chr2_3:3350059-3350078 None:intergenic 15.0%
!!! TATTTCTAAATATTCTTGTT+TGG - chr2_3:3350241-3350260 Msa0271840:CDS 15.0%
!!! AATTCATTTTCATCATTTTT+AGG - chr2_3:3350279-3350298 Msa0271840:CDS 15.0%
!!! AAAAGGTCAATTTATAATTT+TGG - chr2_3:3350381-3350400 Msa0271840:intron 15.0%
!!! ATAATTTTGGAAATTTATTG+GGG - chr2_3:3350394-3350413 Msa0271840:intron 15.0%
!!! TGAAAATTTTGGAAAATTTA+AGG - chr2_3:3350423-3350442 Msa0271840:intron 15.0%
!!! AATTAGTTTCTTAATTAAAC+TGG + chr2_3:3351012-3351031 None:intergenic 15.0%
!! ATAGATTTATAATCATTCAT+TGG - chr2_3:3351040-3351059 Msa0271840:intron 15.0%
!! AATGACAACTTTGATTAAAT+AGG - chr2_3:3350080-3350099 Msa0271840:CDS 20.0%
!! ATGACAACTTTGATTAAATA+GGG - chr2_3:3350081-3350100 Msa0271840:CDS 20.0%
!!! ATTTTGAAAATTGAAAGTAG+AGG - chr2_3:3350333-3350352 Msa0271840:intron 20.0%
!!! TTTAGAAGGAGAATATTTTT+AGG + chr2_3:3350647-3350666 None:intergenic 20.0%
!!! TCATCATCATCATAATTTTT+TGG + chr2_3:3351336-3351355 None:intergenic 20.0%
!! AATGACAACTTTGATTAAAT+AGG - chr2_3:3350080-3350099 Msa0271840:CDS 20.0%
!! ATGACAACTTTGATTAAATA+GGG - chr2_3:3350081-3350100 Msa0271840:CDS 20.0%
!!! ATTTTGAAAATTGAAAGTAG+AGG - chr2_3:3350333-3350352 Msa0271840:intron 20.0%
!!! TTTAGAAGGAGAATATTTTT+AGG + chr2_3:3350647-3350666 None:intergenic 20.0%
!!! TCATCATCATCATAATTTTT+TGG + chr2_3:3351336-3351355 None:intergenic 20.0%
! TTCTTTGTGCATGATTATTT+GGG - chr2_3:3349912-3349931 Msa0271840:three_prime_UTR 25.0%
!!! TTTTTTAGACGCATATTTGA+GGG + chr2_3:3350023-3350042 None:intergenic 25.0%
!!! ATTTTTTAGACGCATATTTG+AGG + chr2_3:3350024-3350043 None:intergenic 25.0%
!!! CATTTTTTTAAGTAGTCTAG+TGG - chr2_3:3350118-3350137 Msa0271840:CDS 25.0%
!! GAGGTAATTGACAAATTTTA+AGG - chr2_3:3350173-3350192 Msa0271840:CDS 25.0%
!! TCTAAATATTCTTGTTTGGA+TGG - chr2_3:3350245-3350264 Msa0271840:CDS 25.0%
!!! CCATTGTTTTGATGATAATT+TGG + chr2_3:3350581-3350600 None:intergenic 25.0%
! CCAAATTATCATCAAAACAA+TGG - chr2_3:3350578-3350597 Msa0271840:intron 25.0%
! ATTGAAAAGATTACCAACTT+CGG - chr2_3:3350825-3350844 Msa0271840:intron 25.0%
!! TTTTCGCTTATAATAATGAC+CGG - chr2_3:3350907-3350926 Msa0271840:intron 25.0%
! AGTTAATCCTAAAAAACCAA+CGG - chr2_3:3351254-3351273 Msa0271840:intron 25.0%
!!! TCATAATTTTTTGGTATCAG+AGG + chr2_3:3351327-3351346 None:intergenic 25.0%
! CATAACTATACAATGTCTTA+AGG + chr2_3:3351424-3351443 None:intergenic 25.0%
! TATCATAAGGACATTGTTTA+AGG + chr2_3:3351507-3351526 None:intergenic 25.0%
!! TTTGCTAACAAAGTATCATA+AGG + chr2_3:3351520-3351539 None:intergenic 25.0%
! TTCTTTGTGCATGATTATTT+GGG - chr2_3:3349912-3349931 Msa0271840:three_prime_UTR 25.0%
!!! TTTTTTAGACGCATATTTGA+GGG + chr2_3:3350023-3350042 None:intergenic 25.0%
!!! ATTTTTTAGACGCATATTTG+AGG + chr2_3:3350024-3350043 None:intergenic 25.0%
!!! CATTTTTTTAAGTAGTCTAG+TGG - chr2_3:3350118-3350137 Msa0271840:CDS 25.0%
!! GAGGTAATTGACAAATTTTA+AGG - chr2_3:3350173-3350192 Msa0271840:CDS 25.0%
!! TCTAAATATTCTTGTTTGGA+TGG - chr2_3:3350245-3350264 Msa0271840:CDS 25.0%
!!! CCATTGTTTTGATGATAATT+TGG + chr2_3:3350581-3350600 None:intergenic 25.0%
! CCAAATTATCATCAAAACAA+TGG - chr2_3:3350578-3350597 Msa0271840:intron 25.0%
! ATTGAAAAGATTACCAACTT+CGG - chr2_3:3350825-3350844 Msa0271840:intron 25.0%
!! TTTTCGCTTATAATAATGAC+CGG - chr2_3:3350907-3350926 Msa0271840:intron 25.0%
! AGTTAATCCTAAAAAACCAA+CGG - chr2_3:3351254-3351273 Msa0271840:intron 25.0%
!!! TCATAATTTTTTGGTATCAG+AGG + chr2_3:3351327-3351346 None:intergenic 25.0%
! CATAACTATACAATGTCTTA+AGG + chr2_3:3351424-3351443 None:intergenic 25.0%
! TATCATAAGGACATTGTTTA+AGG + chr2_3:3351507-3351526 None:intergenic 25.0%
!! TTTGCTAACAAAGTATCATA+AGG + chr2_3:3351520-3351539 None:intergenic 25.0%
CTTCTTTGTGCATGATTATT+TGG - chr2_3:3349911-3349930 Msa0271840:three_prime_UTR 30.0%
TCTTTGTGCATGATTATTTG+GGG - chr2_3:3349913-3349932 Msa0271840:three_prime_UTR 30.0%
TTCAACTCTTAAAGTGTGTT+TGG - chr2_3:3350148-3350167 Msa0271840:CDS 30.0%
! TGGGGTCAATTTGAAAATTT+TGG - chr2_3:3350412-3350431 Msa0271840:intron 30.0%
! TTAGGTGACGTAATATTTTG+AGG + chr2_3:3350629-3350648 None:intergenic 30.0%
! CATGTGAACAAAGATTAGTT+GGG + chr2_3:3350741-3350760 None:intergenic 30.0%
AAGTTTATGCAATGAAACCA+TGG - chr2_3:3351148-3351167 Msa0271840:intron 30.0%
AGTTTATGCAATGAAACCAT+GGG - chr2_3:3351149-3351168 Msa0271840:intron 30.0%
GTTAATCCTAAAAAACCAAC+GGG - chr2_3:3351255-3351274 Msa0271840:intron 30.0%
! TGAGTGGTGATTTTTCTTTT+TGG - chr2_3:3351381-3351400 Msa0271840:CDS 30.0%
CTTCTTTGTGCATGATTATT+TGG - chr2_3:3349911-3349930 Msa0271840:three_prime_UTR 30.0%
TCTTTGTGCATGATTATTTG+GGG - chr2_3:3349913-3349932 Msa0271840:three_prime_UTR 30.0%
TTCAACTCTTAAAGTGTGTT+TGG - chr2_3:3350148-3350167 Msa0271840:CDS 30.0%
! TGGGGTCAATTTGAAAATTT+TGG - chr2_3:3350412-3350431 Msa0271840:intron 30.0%
! TTAGGTGACGTAATATTTTG+AGG + chr2_3:3350629-3350648 None:intergenic 30.0%
! CATGTGAACAAAGATTAGTT+GGG + chr2_3:3350741-3350760 None:intergenic 30.0%
AAGTTTATGCAATGAAACCA+TGG - chr2_3:3351148-3351167 Msa0271840:intron 30.0%
AGTTTATGCAATGAAACCAT+GGG - chr2_3:3351149-3351168 Msa0271840:intron 30.0%
GTTAATCCTAAAAAACCAAC+GGG - chr2_3:3351255-3351274 Msa0271840:intron 30.0%
! TGAGTGGTGATTTTTCTTTT+TGG - chr2_3:3351381-3351400 Msa0271840:CDS 30.0%
!!! TTTTTAAGTAGTCTAGTGGC+TGG - chr2_3:3350122-3350141 Msa0271840:CDS 35.0%
TCTTAAAGTGTGTTTGGATG+AGG - chr2_3:3350154-3350173 Msa0271840:CDS 35.0%
TCCTTCTAAACACACTCTTA+AGG - chr2_3:3350657-3350676 Msa0271840:intron 35.0%
! ACCTTAAGAGTGTGTTTAGA+AGG + chr2_3:3350661-3350680 None:intergenic 35.0%
GTGAATAAGAGAGAAATTCG+AGG - chr2_3:3350679-3350698 Msa0271840:intron 35.0%
! GAACAAAGATTAGTTGGGTA+GGG + chr2_3:3350736-3350755 None:intergenic 35.0%
! TGAACAAAGATTAGTTGGGT+AGG + chr2_3:3350737-3350756 None:intergenic 35.0%
CCATGTGAACAAAGATTAGT+TGG + chr2_3:3350742-3350761 None:intergenic 35.0%
CCAACTAATCTTTGTTCACA+TGG - chr2_3:3350739-3350758 Msa0271840:intron 35.0%
TCATAGTGCATGTGAAAACA+TGG + chr2_3:3350867-3350886 None:intergenic 35.0%
GTTTATGCAATGAAACCATG+GGG - chr2_3:3351150-3351169 Msa0271840:intron 35.0%
TAACCATCATTTGTCGATGT+TGG + chr2_3:3351207-3351226 None:intergenic 35.0%
GATGAAGATCAACTTGACAA+TGG - chr2_3:3351351-3351370 Msa0271840:intron 35.0%
!!! TTTTTAAGTAGTCTAGTGGC+TGG - chr2_3:3350122-3350141 Msa0271840:CDS 35.0%
TCTTAAAGTGTGTTTGGATG+AGG - chr2_3:3350154-3350173 Msa0271840:CDS 35.0%
TCCTTCTAAACACACTCTTA+AGG - chr2_3:3350657-3350676 Msa0271840:intron 35.0%
! ACCTTAAGAGTGTGTTTAGA+AGG + chr2_3:3350661-3350680 None:intergenic 35.0%
GTGAATAAGAGAGAAATTCG+AGG - chr2_3:3350679-3350698 Msa0271840:intron 35.0%
! GAACAAAGATTAGTTGGGTA+GGG + chr2_3:3350736-3350755 None:intergenic 35.0%
! TGAACAAAGATTAGTTGGGT+AGG + chr2_3:3350737-3350756 None:intergenic 35.0%
CCATGTGAACAAAGATTAGT+TGG + chr2_3:3350742-3350761 None:intergenic 35.0%
CCAACTAATCTTTGTTCACA+TGG - chr2_3:3350739-3350758 Msa0271840:intron 35.0%
TCATAGTGCATGTGAAAACA+TGG + chr2_3:3350867-3350886 None:intergenic 35.0%
GTTTATGCAATGAAACCATG+GGG - chr2_3:3351150-3351169 Msa0271840:intron 35.0%
TAACCATCATTTGTCGATGT+TGG + chr2_3:3351207-3351226 None:intergenic 35.0%
GATGAAGATCAACTTGACAA+TGG - chr2_3:3351351-3351370 Msa0271840:intron 35.0%
TTAGACGCATATTTGAGGGT+GGG + chr2_3:3350019-3350038 None:intergenic 40.0%
TTTAGACGCATATTTGAGGG+TGG + chr2_3:3350020-3350039 None:intergenic 40.0%
TAGGGACGTTACATTATCCA+GGG + chr2_3:3350718-3350737 None:intergenic 40.0%
TCGCTTATAATAATGACCGG+AGG - chr2_3:3350910-3350929 Msa0271840:intron 40.0%
ATGTACACATTAGGGACTCA+TGG - chr2_3:3350933-3350952 Msa0271840:intron 40.0%
GATCCAACATCGACAAATGA+TGG - chr2_3:3351201-3351220 Msa0271840:intron 40.0%
ATCAGAGGTTTATGCGTTTG+CGG + chr2_3:3351312-3351331 None:intergenic 40.0%
TTAGACGCATATTTGAGGGT+GGG + chr2_3:3350019-3350038 None:intergenic 40.0%
TTTAGACGCATATTTGAGGG+TGG + chr2_3:3350020-3350039 None:intergenic 40.0%
TAGGGACGTTACATTATCCA+GGG + chr2_3:3350718-3350737 None:intergenic 40.0%
TCGCTTATAATAATGACCGG+AGG - chr2_3:3350910-3350929 Msa0271840:intron 40.0%
ATGTACACATTAGGGACTCA+TGG - chr2_3:3350933-3350952 Msa0271840:intron 40.0%
GATCCAACATCGACAAATGA+TGG - chr2_3:3351201-3351220 Msa0271840:intron 40.0%
ATCAGAGGTTTATGCGTTTG+CGG + chr2_3:3351312-3351331 None:intergenic 40.0%
TTTCAAATGGCTCTTGCAGC+AGG - chr2_3:3349968-3349987 Msa0271840:three_prime_UTR 45.0%
GTAGGGACGTTACATTATCC+AGG + chr2_3:3350719-3350738 None:intergenic 45.0%
ACCGGAGGATGTACACATTA+GGG - chr2_3:3350925-3350944 Msa0271840:intron 45.0%
TCCCTAATGTGTACATCCTC+CGG + chr2_3:3350929-3350948 None:intergenic 45.0%
!!! GTGCTACCCGTTGGTTTTTT+AGG + chr2_3:3351264-3351283 None:intergenic 45.0%
AAAAAACCAACGGGTAGCAC+AGG - chr2_3:3351264-3351283 Msa0271840:intron 45.0%
TGACAATGGCAATGAGTGAG+TGG - chr2_3:3351365-3351384 Msa0271840:intron 45.0%
TTTCAAATGGCTCTTGCAGC+AGG - chr2_3:3349968-3349987 Msa0271840:three_prime_UTR 45.0%
GTAGGGACGTTACATTATCC+AGG + chr2_3:3350719-3350738 None:intergenic 45.0%
ACCGGAGGATGTACACATTA+GGG - chr2_3:3350925-3350944 Msa0271840:intron 45.0%
TCCCTAATGTGTACATCCTC+CGG + chr2_3:3350929-3350948 None:intergenic 45.0%
!!! GTGCTACCCGTTGGTTTTTT+AGG + chr2_3:3351264-3351283 None:intergenic 45.0%
AAAAAACCAACGGGTAGCAC+AGG - chr2_3:3351264-3351283 Msa0271840:intron 45.0%
TGACAATGGCAATGAGTGAG+TGG - chr2_3:3351365-3351384 Msa0271840:intron 45.0%
GACCGGAGGATGTACACATT+AGG - chr2_3:3350924-3350943 Msa0271840:intron 50.0%
ATGAAACCATGGGGTAGCAC+AGG - chr2_3:3351159-3351178 Msa0271840:intron 50.0%
GACCGGAGGATGTACACATT+AGG - chr2_3:3350924-3350943 Msa0271840:intron 50.0%
ATGAAACCATGGGGTAGCAC+AGG - chr2_3:3351159-3351178 Msa0271840:intron 50.0%
TATTTGAGGGTGGGCAGCAC+TGG + chr2_3:3350010-3350029 None:intergenic 55.0%
GAGGTTCGAACTTCGATCCC+TGG - chr2_3:3350698-3350717 Msa0271840:intron 55.0%
TACCAACTTCGGTCACGACC+AGG - chr2_3:3350836-3350855 Msa0271840:intron 55.0%
TGTGAAAACATGGAGCCGCC+TGG + chr2_3:3350857-3350876 None:intergenic 55.0%
AGCACAGGAAGCGGAGGATT+CGG - chr2_3:3351174-3351193 Msa0271840:intron 55.0%
TAGCACAGGAAGCGGAGGAT+TGG - chr2_3:3351173-3351192 Msa0271840:intron 55.0%
AGCACAGGAAGCGGAGGATT+GGG - chr2_3:3351174-3351193 Msa0271840:intron 55.0%
TATTTGAGGGTGGGCAGCAC+TGG + chr2_3:3350010-3350029 None:intergenic 55.0%
GAGGTTCGAACTTCGATCCC+TGG - chr2_3:3350698-3350717 Msa0271840:intron 55.0%
TACCAACTTCGGTCACGACC+AGG - chr2_3:3350836-3350855 Msa0271840:intron 55.0%
TGTGAAAACATGGAGCCGCC+TGG + chr2_3:3350857-3350876 None:intergenic 55.0%
AGCACAGGAAGCGGAGGATT+CGG - chr2_3:3351174-3351193 Msa0271840:intron 55.0%
TAGCACAGGAAGCGGAGGAT+TGG - chr2_3:3351173-3351192 Msa0271840:intron 55.0%
AGCACAGGAAGCGGAGGATT+GGG - chr2_3:3351174-3351193 Msa0271840:intron 55.0%
CAACTTCGGTCACGACCAGG+CGG - chr2_3:3350839-3350858 Msa0271840:intron 60.0%
CCATGGGGTAGCACAGGAAG+CGG - chr2_3:3351165-3351184 Msa0271840:intron 60.0%
GCACAGGAAGCGGAGGATTC+GGG - chr2_3:3351175-3351194 Msa0271840:intron 60.0%
AGGAAGCGGAGGATTCGGGA+AGG - chr2_3:3351179-3351198 Msa0271840:intron 60.0%
CCAACGGGTAGCACAGGAAG+CGG - chr2_3:3351270-3351289 Msa0271840:intron 60.0%
GCACAGGAAGCGGAGGATTG+GGG - chr2_3:3351280-3351299 Msa0271840:intron 60.0%
CAACTTCGGTCACGACCAGG+CGG - chr2_3:3350839-3350858 Msa0271840:intron 60.0%
CCATGGGGTAGCACAGGAAG+CGG - chr2_3:3351165-3351184 Msa0271840:intron 60.0%
GCACAGGAAGCGGAGGATTC+GGG - chr2_3:3351175-3351194 Msa0271840:intron 60.0%
AGGAAGCGGAGGATTCGGGA+AGG - chr2_3:3351179-3351198 Msa0271840:intron 60.0%
CCAACGGGTAGCACAGGAAG+CGG - chr2_3:3351270-3351289 Msa0271840:intron 60.0%
GCACAGGAAGCGGAGGATTG+GGG - chr2_3:3351280-3351299 Msa0271840:intron 60.0%
!! GGTGGGCAGCACTGGCATGT+GGG + chr2_3:3350002-3350021 None:intergenic 65.0%
CGCCTGGTCGTGACCGAAGT+TGG + chr2_3:3350841-3350860 None:intergenic 65.0%
CCGCTTCCTGTGCTACCCCA+TGG + chr2_3:3351168-3351187 None:intergenic 65.0%
TGGGGTAGCACAGGAAGCGG+AGG - chr2_3:3351168-3351187 Msa0271840:intron 65.0%
CCGCTTCCTGTGCTACCCGT+TGG + chr2_3:3351273-3351292 None:intergenic 65.0%
ACGGGTAGCACAGGAAGCGG+AGG - chr2_3:3351273-3351292 Msa0271840:intron 65.0%
!! GGTGGGCAGCACTGGCATGT+GGG + chr2_3:3350002-3350021 None:intergenic 65.0%
CGCCTGGTCGTGACCGAAGT+TGG + chr2_3:3350841-3350860 None:intergenic 65.0%
CCGCTTCCTGTGCTACCCCA+TGG + chr2_3:3351168-3351187 None:intergenic 65.0%
TGGGGTAGCACAGGAAGCGG+AGG - chr2_3:3351168-3351187 Msa0271840:intron 65.0%
CCGCTTCCTGTGCTACCCGT+TGG + chr2_3:3351273-3351292 None:intergenic 65.0%
ACGGGTAGCACAGGAAGCGG+AGG - chr2_3:3351273-3351292 Msa0271840:intron 65.0%
!! GGGTGGGCAGCACTGGCATG+TGG + chr2_3:3350003-3350022 None:intergenic 70.0%
!! GGGTGGGCAGCACTGGCATG+TGG + chr2_3:3350003-3350022 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 3349875 3351555 3349875 ID=Msa0271840;Name=Msa0271840
chr2_3 mRNA 3349875 3351555 3349875 ID=Msa0271840-mRNA-1;Parent=Msa0271840;Name=Msa0271840-mRNA-1;_AED=0.30;_eAED=0.30;_QI=99|1|1|1|1|1|2|172|117
chr2_3 exon 3349875 3350314 3349875 ID=Msa0271840-mRNA-1:exon:1686;Parent=Msa0271840-mRNA-1
chr2_3 exon 3351371 3351555 3351371 ID=Msa0271840-mRNA-1:exon:1685;Parent=Msa0271840-mRNA-1
chr2_3 five_prime_UTR 3351457 3351555 3351457 ID=Msa0271840-mRNA-1:five_prime_utr;Parent=Msa0271840-mRNA-1
chr2_3 CDS 3351371 3351456 3351371 ID=Msa0271840-mRNA-1:cds;Parent=Msa0271840-mRNA-1
chr2_3 CDS 3350047 3350314 3350047 ID=Msa0271840-mRNA-1:cds;Parent=Msa0271840-mRNA-1
chr2_3 three_prime_UTR 3349875 3350046 3349875 ID=Msa0271840-mRNA-1:three_prime_utr;Parent=Msa0271840-mRNA-1
Gene Sequence

>Msa0271840

ATGGCTCTTGCAGCAGGTAGCTTCACCCACATGCCAGTGCTGCCCACCCTCAAATATGCGTCTAAAAAATCTATGACAATTACCATAAAATGTGCTAACAACAACAAAAACAAAACAAAAGTTTATGCAATGAAACCATGGGGTAGCACAGGAAGCGGAGGATTCGGGAAGGATCCAACATCGACAAATGATGGTTATGATGAACATGACGAAAACAAAATAAAAGTTAATCCTAAAAAACCAACGGGTAGCACAGGAAGCGGAGGATTGGGGACTGAACCGCAAACGCATAAACCTCTGATACCAAAAAATTATGATGATGATGAAGATCAACTTGACAATGGCAATGAGTGA
Protein sequence

>Msa0271840

MALAAGSFTHMPVLPTLKYASKKSMTITIKCANNNKNKTKVYAMKPWGSTGSGGFGKDPTSTNDGYDEHDENKIKVNPKKPTGSTGSGGLGTEPQTHKPLIPKNYDDDEDQLDNGNE*