AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0288180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0288180 MtrunA17_Chr2g0305851 76.923 221 8 2 1 178 1 221 1.54e-112 319
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0288180 AT5G57950.1 48.684 228 66 4 1 178 1 227 1.51e-63 195
Msa0288180 AT5G57950.2 48.684 228 66 4 1 178 30 256 3.88e-63 195

Find 71 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCAAGGCAGAGGTTTAC+TGG 0.266830 2_3:+32280395 Msa0288180:CDS
CCTGGCGCCCCTGGACTTTC+CGG 0.273188 2_3:+32279279 Msa0288180:CDS
GGAAGGTTAGATGAAAAGTT+AGG 0.296745 2_3:+32279118 Msa0288180:five_prime_UTR
ACGATCAAAATAAACTAAAA+AGG 0.317401 2_3:-32282643 None:intergenic
TTCTGTTGGATGGATGTAAA+AGG 0.329244 2_3:+32282683 Msa0288180:three_prime_UTR
TAACAGCTGACCTTTGATTA+TGG 0.335437 2_3:+32282589 Msa0288180:three_prime_UTR
TTGTTTGACCCATGCTCGAC+TGG 0.343385 2_3:-32280314 None:intergenic
AGATTCAAGATTTAGGAAAT+AGG 0.348455 2_3:+32279774 Msa0288180:intron
TCTCAATTCCCTGGCGCCCC+TGG 0.352289 2_3:+32279270 Msa0288180:CDS
CTCAGAATCAATTAGATTGC+CGG 0.364511 2_3:-32279298 None:intergenic
TTAGATGAAAAGTTAGGGTT+TGG 0.375084 2_3:+32279124 Msa0288180:five_prime_UTR
AGCAGAAACAAGATCGCTAA+TGG 0.381813 2_3:+32279197 Msa0288180:CDS
GCATCTACAAGATCCGAAAA+TGG 0.385894 2_3:-32282448 None:intergenic
TTGTTAGAGGTATCTTCTGT+TGG 0.409087 2_3:+32282669 Msa0288180:three_prime_UTR
ACATCCACATCCATCGAGTT+GGG 0.410980 2_3:-32280144 None:intergenic
ACCCATGCTCGACTGGGCCT+CGG 0.418523 2_3:-32280307 None:intergenic
CCGGAAAGTCCAGGGGCGCC+AGG 0.421638 2_3:-32279279 None:intergenic
GAAGGTTAGATGAAAAGTTA+GGG 0.429600 2_3:+32279119 Msa0288180:five_prime_UTR
GCTCGTCTTTCTCAATTCCC+TGG 0.439681 2_3:+32279261 Msa0288180:CDS
GTGATCGTTTGTTTCTTCTC+CGG 0.447543 2_3:+32282490 Msa0288180:three_prime_UTR
TTCCGAGGCCCAGTCGAGCA+TGG 0.452146 2_3:+32280305 Msa0288180:CDS
TGAAAATTTGCTCCATAGGT+TGG 0.465291 2_3:+32280281 Msa0288180:CDS
ATCTCGTCAACCACTGCAAA+AGG 0.466046 2_3:-32280180 None:intergenic
AAATTTGGCAACGTCGAAGC+AGG 0.480789 2_3:+32280258 Msa0288180:CDS
TAGAGGTATCTTCTGTTGGA+TGG 0.481623 2_3:+32282673 Msa0288180:three_prime_UTR
ATTAGATTGCCGGAAAGTCC+AGG 0.487468 2_3:-32279288 None:intergenic
CCTCTGCCTTGCCAAGTCCT+AGG 0.496210 2_3:-32280387 None:intergenic
CGGCAATCTAATTGATTCTG+AGG 0.497483 2_3:+32279299 Msa0288180:CDS
TTCCGCTAAGACAATGATGA+TGG 0.500406 2_3:+32280045 Msa0288180:intron
GTAGAAGATGGTTTACAGCT+TGG 0.500978 2_3:+32280222 Msa0288180:CDS
GGGCCTCGGAAGCCAACCTA+TGG 0.501119 2_3:-32280293 None:intergenic
CAGGTGAAAATTTGCTCCAT+AGG 0.501246 2_3:+32280277 Msa0288180:CDS
GAAAATGAAAATAAATACAA+AGG 0.503653 2_3:+32279069 Msa0288180:five_prime_UTR
TGGCAAGGCAGAGGTTTACT+GGG 0.504955 2_3:+32280396 Msa0288180:CDS
CTCGATGGATGTGGATGTCT+TGG 0.513868 2_3:+32280149 Msa0288180:CDS
GACATGCAGACCTCATCTAC+TGG 0.518074 2_3:+32280072 Msa0288180:CDS
ATACAAAGACAAGTCACAAA+AGG 0.520389 2_3:-32282526 None:intergenic
TCCGAGGCCCAGTCGAGCAT+GGG 0.530910 2_3:+32280306 Msa0288180:CDS
GACATCCACATCCATCGAGT+TGG 0.533268 2_3:-32280145 None:intergenic
AAAATGAAAATAAATACAAA+GGG 0.535521 2_3:+32279070 Msa0288180:five_prime_UTR
CCTTGCCTCATGATGACAAC+AGG 0.536038 2_3:-32280339 None:intergenic
GCTCCATAGGTTGGCTTCCG+AGG 0.537445 2_3:+32280290 Msa0288180:CDS
AAAAGGTCAAGAACAAATTC+CGG 0.543057 2_3:-32282509 None:intergenic
GATGTAAAAGGAATGGGAAA+CGG 0.549712 2_3:+32282695 Msa0288180:three_prime_UTR
TGTTTGACCCATGCTCGACT+GGG 0.552241 2_3:-32280313 None:intergenic
GCATCACCAGCGGTAGAAGA+TGG 0.559452 2_3:+32280210 Msa0288180:CDS
CCTAGGACTTGGCAAGGCAG+AGG 0.560284 2_3:+32280387 Msa0288180:CDS
TTGGATGGATGTAAAAGGAA+TGG 0.562878 2_3:+32282688 Msa0288180:three_prime_UTR
AAAGCAGAGTAGAAGAAGAA+TGG 0.567060 2_3:+32279158 Msa0288180:exon
TGTAAACCATCTTCTACCGC+TGG 0.569610 2_3:-32280216 None:intergenic
CAGAGTAGAAGAAGAATGGT+GGG 0.571601 2_3:+32279162 Msa0288180:exon
CGGAAAGTCCAGGGGCGCCA+GGG 0.574777 2_3:-32279278 None:intergenic
TAACTGTTACACCTAGGACT+TGG 0.577662 2_3:+32280376 Msa0288180:CDS
CATCCACATCCATCGAGTTG+GGG 0.579168 2_3:-32280143 None:intergenic
TGGATGGATGTAAAAGGAAT+GGG 0.584263 2_3:+32282689 Msa0288180:three_prime_UTR
TTAGATTGCCGGAAAGTCCA+GGG 0.588856 2_3:-32279287 None:intergenic
AATAAATACAAAGGGAACGC+AGG 0.591051 2_3:+32279078 Msa0288180:five_prime_UTR
TGCTGAGTGTGTCGTAATGT+TGG 0.599036 2_3:+32282468 Msa0288180:three_prime_UTR
CAATACCTGTTGTCATCATG+AGG 0.605657 2_3:+32280334 Msa0288180:CDS
AACAGTCTCTCCAGTAGATG+AGG 0.614905 2_3:-32280082 None:intergenic
GCAGAGTAGAAGAAGAATGG+TGG 0.622593 2_3:+32279161 Msa0288180:exon
CAGCTGACCTTTGATTATGG+TGG 0.622700 2_3:+32282592 Msa0288180:three_prime_UTR
CCTGTTGTCATCATGAGGCA+AGG 0.626933 2_3:+32280339 Msa0288180:CDS
TAATTGACCACCATAATCAA+AGG 0.636905 2_3:-32282599 None:intergenic
GTTACACCTAGGACTTGGCA+AGG 0.647181 2_3:+32280381 Msa0288180:CDS
CTCACAAGGACATTCTGCAA+TGG 0.656644 2_3:-32280114 None:intergenic
ATCCACATCCATCGAGTTGG+GGG 0.663720 2_3:-32280142 None:intergenic
TCAACTTAACTGTTACACCT+AGG 0.680007 2_3:+32280370 Msa0288180:CDS
TAGATTGCCGGAAAGTCCAG+GGG 0.688081 2_3:-32279286 None:intergenic
GGCAAGGCAGAGGTTTACTG+GGG 0.698130 2_3:+32280397 Msa0288180:CDS
GATATCAGATGCATCACCAG+CGG 0.721340 2_3:+32280200 Msa0288180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATGAAAATAAATACAAA+GGG + chr2_3:32279070-32279089 Msa0288180:five_prime_UTR 10.0%
!! TAACAAATAACAAAATAATT+GGG - chr2_3:32280435-32280454 None:intergenic 10.0%
!! ATAACAAATAACAAAATAAT+TGG - chr2_3:32280436-32280455 None:intergenic 10.0%
!!! TTTAGTTTTATCTTTTAATT+CGG - chr2_3:32280740-32280759 None:intergenic 10.0%
!!! TTTTAAAAAAAATGGTATTT+TGG + chr2_3:32281695-32281714 Msa0288180:intron 10.0%
!! GAAAATGAAAATAAATACAA+AGG + chr2_3:32279069-32279088 Msa0288180:five_prime_UTR 15.0%
!!! TCATTTTCTTGAATTATTAT+CGG - chr2_3:32279641-32279660 None:intergenic 15.0%
!! TATTTATATAAGAATCTGTT+AGG - chr2_3:32281123-32281142 None:intergenic 15.0%
!!! ACTTCTAATATTTTTACTTT+TGG + chr2_3:32281221-32281240 Msa0288180:intron 15.0%
!! TTAGAGAAAAAAAAAAAAGT+CGG - chr2_3:32281349-32281368 None:intergenic 15.0%
!!! TTTATTTTTCCTTTTCTAAT+CGG + chr2_3:32281539-32281558 Msa0288180:intron 15.0%
!!! ATATTTTCTACATATATGAT+CGG - chr2_3:32282343-32282362 None:intergenic 15.0%
!! TAACTACTAATTTAAATTAG+TGG + chr2_3:32282367-32282386 Msa0288180:intron 15.0%
!! ATTAATCTTATCAGTTATGT+CGG - chr2_3:32279497-32279516 None:intergenic 20.0%
!! AGATTTCTATGTAGAATAAA+TGG - chr2_3:32280583-32280602 None:intergenic 20.0%
!!! TTTTTACTTTTGGTTACAAA+TGG + chr2_3:32281231-32281250 Msa0288180:intron 20.0%
!!! CTAATAAATTTTAGCTTGAA+TGG + chr2_3:32281365-32281384 Msa0288180:intron 20.0%
!!! TCGAATTATAAAGTATTTTC+TGG - chr2_3:32281518-32281537 None:intergenic 20.0%
!!! CGATCTACTTTTAAAAAAAA+TGG + chr2_3:32281687-32281706 Msa0288180:intron 20.0%
!!! TGAAAACTTCTTTTGAAAAA+TGG + chr2_3:32281747-32281766 Msa0288180:intron 20.0%
!!! GTTGTTTTACTTATAACTTA+TGG + chr2_3:32281928-32281947 Msa0288180:intron 20.0%
!!! ATATATAAACCTTTTAATGC+TGG - chr2_3:32282068-32282087 None:intergenic 20.0%
!! AGCATTAAAAGGTTTATATA+TGG + chr2_3:32282067-32282086 Msa0288180:intron 20.0%
!!! GTAAATTGTTTCTTGTAAAT+AGG - chr2_3:32282161-32282180 None:intergenic 20.0%
!! TATAAACTCTGTTCTAATTA+TGG + chr2_3:32282306-32282325 Msa0288180:intron 20.0%
!! ATAAACTCTGTTCTAATTAT+GGG + chr2_3:32282307-32282326 Msa0288180:intron 20.0%
!! ACGATCAAAATAAACTAAAA+AGG - chr2_3:32282646-32282665 None:intergenic 20.0%
! CTTGGAAATCATATAAATTC+AGG + chr2_3:32279547-32279566 Msa0288180:intron 25.0%
! GAAATCATATAAATTCAGGT+TGG + chr2_3:32279551-32279570 Msa0288180:intron 25.0%
! ACATAATTCAATGTAAACAC+AGG + chr2_3:32279660-32279679 Msa0288180:intron 25.0%
! TAAGATCAGATTCAAGATTT+AGG + chr2_3:32279767-32279786 Msa0288180:intron 25.0%
! AGATTCAAGATTTAGGAAAT+AGG + chr2_3:32279774-32279793 Msa0288180:intron 25.0%
!!! GGAGATCAAATTTTGAAATT+TGG + chr2_3:32280243-32280262 Msa0288180:CDS 25.0%
! AATAACAAAATAATTGGGAC+AGG - chr2_3:32280430-32280449 None:intergenic 25.0%
! ATGTAGAATAAATGGATCAT+CGG - chr2_3:32280575-32280594 None:intergenic 25.0%
! GTTATTTATCTGATTCTGAT+AGG + chr2_3:32280711-32280730 Msa0288180:intron 25.0%
! GAAAATTAGACTAAAGAACT+AGG - chr2_3:32280865-32280884 None:intergenic 25.0%
!!! TGCAGTTATTTGTATTGATT+AGG - chr2_3:32280960-32280979 None:intergenic 25.0%
! ACAAAAGAAATATCAGCTAA+AGG - chr2_3:32281150-32281169 None:intergenic 25.0%
! CTTCTTATATTGAAAGTGAT+TGG + chr2_3:32281580-32281599 Msa0288180:intron 25.0%
! AATCTTCTTCTATAATGGTA+CGG - chr2_3:32281630-32281649 None:intergenic 25.0%
! TACCATTATAGAAGAAGATT+CGG + chr2_3:32281630-32281649 Msa0288180:intron 25.0%
! GTTCTATCAAAAACCATATT+TGG - chr2_3:32281842-32281861 None:intergenic 25.0%
! AATTAGTGTTAACTGGATTA+GGG - chr2_3:32282011-32282030 None:intergenic 25.0%
! AAATTAGTGTTAACTGGATT+AGG - chr2_3:32282012-32282031 None:intergenic 25.0%
!! AATCCAGTTAACACTAATTT+TGG + chr2_3:32282012-32282031 Msa0288180:intron 25.0%
! GATTTCAGTTCTTAAGTAAT+TGG + chr2_3:32282102-32282121 Msa0288180:intron 25.0%
GAAGGTTAGATGAAAAGTTA+GGG + chr2_3:32279119-32279138 Msa0288180:five_prime_UTR 30.0%
TTAGATGAAAAGTTAGGGTT+TGG + chr2_3:32279124-32279143 Msa0288180:five_prime_UTR 30.0%
TATATGATTTCCAAGTCTTG+AGG - chr2_3:32279542-32279561 None:intergenic 30.0%
TCTTGAATTATTATCGGTGT+CGG - chr2_3:32279635-32279654 None:intergenic 30.0%
! GAAACTTCACAATCAGATTT+TGG - chr2_3:32279832-32279851 None:intergenic 30.0%
! ACGTTCTAAACAAAACTGAA+AGG - chr2_3:32280477-32280496 None:intergenic 30.0%
ATTAGACTAAAGAACTAGGA+AGG - chr2_3:32280861-32280880 None:intergenic 30.0%
GTATTATTAGCAAGTCCATT+CGG - chr2_3:32280911-32280930 None:intergenic 30.0%
ATTGATTAGGATCAGCTTAT+AGG - chr2_3:32280947-32280966 None:intergenic 30.0%
!! TGCAGTCATTTGTATTGATT+AGG - chr2_3:32281007-32281026 None:intergenic 30.0%
TTAAATAGAAGCAGATCAAG+AGG - chr2_3:32281183-32281202 None:intergenic 30.0%
CTTGATCTGCTTCTATTTAA+AGG + chr2_3:32281183-32281202 Msa0288180:intron 30.0%
!! TTACTTTTGGTTACAAATGG+AGG + chr2_3:32281234-32281253 Msa0288180:intron 30.0%
!! AGCTATTGAATTTGTTGAAC+GGG - chr2_3:32281483-32281502 None:intergenic 30.0%
!! CAGCTATTGAATTTGTTGAA+CGG - chr2_3:32281484-32281503 None:intergenic 30.0%
CAACAAATTCAATAGCTGTT+TGG + chr2_3:32281486-32281505 Msa0288180:intron 30.0%
TTCAATATAAGAAGTCGAGT+AGG - chr2_3:32281574-32281593 None:intergenic 30.0%
ACCATTATAGAAGAAGATTC+GGG + chr2_3:32281631-32281650 Msa0288180:intron 30.0%
!! GAAGTTTTCATTGAGTATTG+AGG - chr2_3:32281737-32281756 None:intergenic 30.0%
TGACCAAAATTAGTGTTAAC+TGG - chr2_3:32282018-32282037 None:intergenic 30.0%
ACACTATTTCCAGCATTAAA+AGG + chr2_3:32282056-32282075 Msa0288180:intron 30.0%
TTCAGTTCTTAAGTAATTGG+TGG + chr2_3:32282105-32282124 Msa0288180:intron 30.0%
! TTGTTTCTTGTAAATAGGAC+CGG - chr2_3:32282156-32282175 None:intergenic 30.0%
GTCAACTTTCTCTTGTAAAT+AGG + chr2_3:32282260-32282279 Msa0288180:intron 30.0%
TTTCTACATATATGATCGGT+TGG - chr2_3:32282339-32282358 None:intergenic 30.0%
AAAAGGTCAAGAACAAATTC+CGG - chr2_3:32282512-32282531 None:intergenic 30.0%
ATACAAAGACAAGTCACAAA+AGG - chr2_3:32282529-32282548 None:intergenic 30.0%
TAATTGACCACCATAATCAA+AGG - chr2_3:32282602-32282621 None:intergenic 30.0%
! TTTTGATCGTCTTTTGTTAG+AGG + chr2_3:32282656-32282675 Msa0288180:three_prime_UTR 30.0%
AATAAATACAAAGGGAACGC+AGG + chr2_3:32279078-32279097 Msa0288180:five_prime_UTR 35.0%
AACGCAGGAGTAATAAAAAG+AGG + chr2_3:32279093-32279112 Msa0288180:five_prime_UTR 35.0%
ACGCAGGAGTAATAAAAAGA+GGG + chr2_3:32279094-32279113 Msa0288180:five_prime_UTR 35.0%
AGTAATAAAAAGAGGGAGGA+AGG + chr2_3:32279101-32279120 Msa0288180:five_prime_UTR 35.0%
GGAAGGTTAGATGAAAAGTT+AGG + chr2_3:32279118-32279137 Msa0288180:five_prime_UTR 35.0%
AAAGCAGAGTAGAAGAAGAA+TGG + chr2_3:32279158-32279177 Msa0288180:exon 35.0%
CTCAGAATCAATTAGATTGC+CGG - chr2_3:32279301-32279320 None:intergenic 35.0%
! ATTTTGCATTCCTCAAGACT+TGG + chr2_3:32279529-32279548 Msa0288180:intron 35.0%
TTCAATGTAAACACAGGTGT+CGG + chr2_3:32279666-32279685 Msa0288180:intron 35.0%
GTACTTGTATTACTGAAAGG+CGG - chr2_3:32279859-32279878 None:intergenic 35.0%
GCTGTACTTGTATTACTGAA+AGG - chr2_3:32279862-32279881 None:intergenic 35.0%
! TGAAAATTTGCTCCATAGGT+TGG + chr2_3:32280281-32280300 Msa0288180:CDS 35.0%
TCAACTTAACTGTTACACCT+AGG + chr2_3:32280370-32280389 Msa0288180:CDS 35.0%
! AAATGGATCATCGGAGTATT+GGG - chr2_3:32280566-32280585 None:intergenic 35.0%
TAAATGGATCATCGGAGTAT+TGG - chr2_3:32280567-32280586 None:intergenic 35.0%
! TTTTCTCCGATGATCCATTT+AGG + chr2_3:32280629-32280648 Msa0288180:intron 35.0%
TTTCTCCGATGATCCATTTA+GGG + chr2_3:32280630-32280649 Msa0288180:intron 35.0%
!! TGTATTGATTAGGAGCAGTT+AGG - chr2_3:32280997-32281016 None:intergenic 35.0%
TTGGGTTTAATAACTGCATC+AGG + chr2_3:32281064-32281083 Msa0288180:intron 35.0%
!! AGGTATCAGTTGGTATTGAT+AGG - chr2_3:32281103-32281122 None:intergenic 35.0%
AGAATCTGTTAGGTATCAGT+TGG - chr2_3:32281113-32281132 None:intergenic 35.0%
AGTCGGATACTAACTATGAA+AGG - chr2_3:32281332-32281351 None:intergenic 35.0%
! TTAGCTTGAATGGTTTGTAG+AGG + chr2_3:32281375-32281394 Msa0288180:intron 35.0%
AAGAAATTGCATGTGTCGAA+TGG - chr2_3:32281419-32281438 None:intergenic 35.0%
AATATAAGAAGTCGAGTAGG+AGG - chr2_3:32281571-32281590 None:intergenic 35.0%
ACCCGAATCTTCTTCTATAA+TGG - chr2_3:32281635-32281654 None:intergenic 35.0%
GGGTAAAATATGCACTTCTT+CGG + chr2_3:32281651-32281670 Msa0288180:intron 35.0%
GGTAAAATATGCACTTCTTC+GGG + chr2_3:32281652-32281671 Msa0288180:intron 35.0%
CCTCGAAAAATATTCACAGA+AGG - chr2_3:32281805-32281824 None:intergenic 35.0%
! CCTTCTGTGAATATTTTTCG+AGG + chr2_3:32281802-32281821 Msa0288180:intron 35.0%
!!! ATTTTTCGAGGTCAATGAGT+TGG + chr2_3:32281814-32281833 Msa0288180:intron 35.0%
! CAATGAGTTGGATCCAAATA+TGG + chr2_3:32281826-32281845 Msa0288180:intron 35.0%
TATGGTCACTTCATCATCTT+AGG + chr2_3:32281946-32281965 Msa0288180:intron 35.0%
ATGGTCACTTCATCATCTTA+GGG + chr2_3:32281947-32281966 Msa0288180:intron 35.0%
!! TTCTTAAGTAATTGGTGGTG+TGG + chr2_3:32282110-32282129 Msa0288180:intron 35.0%
ATGTTATCATATACTCCCTC+CGG + chr2_3:32282134-32282153 Msa0288180:intron 35.0%
CTTTCTCTTGTAAATAGGAC+CGG + chr2_3:32282265-32282284 Msa0288180:intron 35.0%
ATCAAGTATAATACTCCCTC+CGG - chr2_3:32282287-32282306 None:intergenic 35.0%
TAAATTAGTGGAGTTGCTAC+TGG + chr2_3:32282379-32282398 Msa0288180:intron 35.0%
TAACAGCTGACCTTTGATTA+TGG + chr2_3:32282589-32282608 Msa0288180:three_prime_UTR 35.0%
!! TTGTTAGAGGTATCTTCTGT+TGG + chr2_3:32282669-32282688 Msa0288180:three_prime_UTR 35.0%
TTCTGTTGGATGGATGTAAA+AGG + chr2_3:32282683-32282702 Msa0288180:three_prime_UTR 35.0%
TTGGATGGATGTAAAAGGAA+TGG + chr2_3:32282688-32282707 Msa0288180:three_prime_UTR 35.0%
TGGATGGATGTAAAAGGAAT+GGG + chr2_3:32282689-32282708 Msa0288180:three_prime_UTR 35.0%
CAGGAGTAATAAAAAGAGGG+AGG + chr2_3:32279097-32279116 Msa0288180:five_prime_UTR 40.0%
CAGAGTAGAAGAAGAATGGT+GGG + chr2_3:32279162-32279181 Msa0288180:exon 40.0%
AGCAGAAACAAGATCGCTAA+TGG + chr2_3:32279197-32279216 Msa0288180:CDS 40.0%
! CGGCAATCTAATTGATTCTG+AGG + chr2_3:32279299-32279318 Msa0288180:CDS 40.0%
! AGAGAGAGAGTTATGATGGA+TGG - chr2_3:32279359-32279378 None:intergenic 40.0%
AGAGAGAGAGAGAGTTATGA+TGG - chr2_3:32279363-32279382 None:intergenic 40.0%
AACTAGTGGAACGTCGATAT+CGG - chr2_3:32279431-32279450 None:intergenic 40.0%
GCACAAACACTTCTCGAATT+AGG - chr2_3:32279710-32279729 None:intergenic 40.0%
TTCCGCTAAGACAATGATGA+TGG + chr2_3:32280045-32280064 Msa0288180:intron 40.0%
AGCCATCATCATTGTCTTAG+CGG - chr2_3:32280050-32280069 None:intergenic 40.0%
GTAGAAGATGGTTTACAGCT+TGG + chr2_3:32280222-32280241 Msa0288180:CDS 40.0%
! CAGGTGAAAATTTGCTCCAT+AGG + chr2_3:32280277-32280296 Msa0288180:CDS 40.0%
CAATACCTGTTGTCATCATG+AGG + chr2_3:32280334-32280353 Msa0288180:CDS 40.0%
TAACTGTTACACCTAGGACT+TGG + chr2_3:32280376-32280395 Msa0288180:CDS 40.0%
! TTGGGTTAGACTGGTAAAAG+GGG - chr2_3:32280542-32280561 None:intergenic 40.0%
! CTTGGGTTAGACTGGTAAAA+GGG - chr2_3:32280543-32280562 None:intergenic 40.0%
TGGATCATCGGAGAAAATAC+TGG - chr2_3:32280626-32280645 None:intergenic 40.0%
GAACACCCTAAATGGATCAT+CGG - chr2_3:32280638-32280657 None:intergenic 40.0%
! ATCCATTTAGGGTGTTCAGA+AGG + chr2_3:32280641-32280660 Msa0288180:intron 40.0%
ATCCTTCTGAACACCCTAAA+TGG - chr2_3:32280646-32280665 None:intergenic 40.0%
! TCTGAAGTAGCATTAGCACT+GGG - chr2_3:32280818-32280837 None:intergenic 40.0%
CACAACTGTAGACATGATTC+AGG - chr2_3:32280887-32280906 None:intergenic 40.0%
CTGAATCATGTCTACAGTTG+TGG + chr2_3:32280885-32280904 Msa0288180:intron 40.0%
GAGGTCTAGTAGTATAGAGT+GGG + chr2_3:32281253-32281272 Msa0288180:intron 40.0%
!! CGAATGGATTAGAGAAGGTA+GGG - chr2_3:32281403-32281422 None:intergenic 40.0%
!! TCGAATGGATTAGAGAAGGT+AGG - chr2_3:32281404-32281423 None:intergenic 40.0%
!! TGTGTCGAATGGATTAGAGA+AGG - chr2_3:32281408-32281427 None:intergenic 40.0%
!! AGGATTGGTCCGATTAGAAA+AGG - chr2_3:32281551-32281570 None:intergenic 40.0%
TTCTTGTAAATAGGACCGGA+GGG - chr2_3:32282152-32282171 None:intergenic 40.0%
TTTCTTGTAAATAGGACCGG+AGG - chr2_3:32282153-32282172 None:intergenic 40.0%
!!! TTCTGTGCAGATGCCATTTT+CGG + chr2_3:32282435-32282454 Msa0288180:intron 40.0%
GCATCTACAAGATCCGAAAA+TGG - chr2_3:32282451-32282470 None:intergenic 40.0%
! GTGATCGTTTGTTTCTTCTC+CGG + chr2_3:32282490-32282509 Msa0288180:three_prime_UTR 40.0%
!! TAGAGGTATCTTCTGTTGGA+TGG + chr2_3:32282673-32282692 Msa0288180:three_prime_UTR 40.0%
GCAGAGTAGAAGAAGAATGG+TGG + chr2_3:32279161-32279180 Msa0288180:exon 45.0%
TTAGATTGCCGGAAAGTCCA+GGG - chr2_3:32279290-32279309 None:intergenic 45.0%
ATTAGATTGCCGGAAAGTCC+AGG - chr2_3:32279291-32279310 None:intergenic 45.0%
TCTCTCTCTCTCTCTCTCTA+GGG + chr2_3:32279394-32279413 Msa0288180:intron 45.0%
GTCTCGTGTTAGTGCTTCAT+AGG - chr2_3:32279590-32279609 None:intergenic 45.0%
AACAGTCTCTCCAGTAGATG+AGG - chr2_3:32280085-32280104 None:intergenic 45.0%
CTCACAAGGACATTCTGCAA+TGG - chr2_3:32280117-32280136 None:intergenic 45.0%
ACATCCACATCCATCGAGTT+GGG - chr2_3:32280147-32280166 None:intergenic 45.0%
! GGTTAGTAGACCTTTTGCAG+TGG + chr2_3:32280170-32280189 Msa0288180:CDS 45.0%
ATCTCGTCAACCACTGCAAA+AGG - chr2_3:32280183-32280202 None:intergenic 45.0%
GATATCAGATGCATCACCAG+CGG + chr2_3:32280200-32280219 Msa0288180:CDS 45.0%
TGTAAACCATCTTCTACCGC+TGG - chr2_3:32280219-32280238 None:intergenic 45.0%
AAATTTGGCAACGTCGAAGC+AGG + chr2_3:32280258-32280277 Msa0288180:CDS 45.0%
! GCTTGGGTTAGACTGGTAAA+AGG - chr2_3:32280544-32280563 None:intergenic 45.0%
!! ATCATCGGAGTATTGGGCTT+GGG - chr2_3:32280560-32280579 None:intergenic 45.0%
! CATTAGCACTGGGATTACAG+GGG - chr2_3:32280808-32280827 None:intergenic 45.0%
! GCATTAGCACTGGGATTACA+GGG - chr2_3:32280809-32280828 None:intergenic 45.0%
! AGCATTAGCACTGGGATTAC+AGG - chr2_3:32280810-32280829 None:intergenic 45.0%
!! CTCTGAAGTAGCATTAGCAC+TGG - chr2_3:32280819-32280838 None:intergenic 45.0%
! TGCTCTCAGGCACTTCTTTT+GGG + chr2_3:32281046-32281065 Msa0288180:intron 45.0%
GGAGGTCTAGTAGTATAGAG+TGG + chr2_3:32281252-32281271 Msa0288180:intron 45.0%
GTTGAACGGGCAAGTACAAA+AGG - chr2_3:32281470-32281489 None:intergenic 45.0%
AAGAAGTCGAGTAGGAGGAT+TGG - chr2_3:32281566-32281585 None:intergenic 45.0%
CGGAATAGTCTCGTTGGAAT+AGG - chr2_3:32281610-32281629 None:intergenic 45.0%
ATGGTACGGAATAGTCTCGT+TGG - chr2_3:32281616-32281635 None:intergenic 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - chr2_3:32281895-32281914 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - chr2_3:32281896-32281915 None:intergenic 45.0%
GGTCTAGACAGAGTGATTTC+AGG - chr2_3:32281990-32282009 None:intergenic 45.0%
TCTCTTGTAAATAGGACCGG+AGG + chr2_3:32282268-32282287 Msa0288180:intron 45.0%
CTCTTGTAAATAGGACCGGA+GGG + chr2_3:32282269-32282288 Msa0288180:intron 45.0%
TGCTGAGTGTGTCGTAATGT+TGG + chr2_3:32282468-32282487 Msa0288180:three_prime_UTR 45.0%
CAGCTGACCTTTGATTATGG+TGG + chr2_3:32282592-32282611 Msa0288180:three_prime_UTR 45.0%
!! CATTAAATAATTTATATATT+TGG + chr2_3:32282191-32282210 Msa0288180:intron 5.0%
GCTCGTCTTTCTCAATTCCC+TGG + chr2_3:32279261-32279280 Msa0288180:CDS 50.0%
TAGATTGCCGGAAAGTCCAG+GGG - chr2_3:32279289-32279308 None:intergenic 50.0%
CTCTCTCTCTCTCTCTCTCT+AGG + chr2_3:32279369-32279388 Msa0288180:intron 50.0%
!! GTAAACACAGGTGTCGGTGT+CGG + chr2_3:32279672-32279691 Msa0288180:intron 50.0%
GACATGCAGACCTCATCTAC+TGG + chr2_3:32280072-32280091 Msa0288180:CDS 50.0%
AGTTGGGGGAAAGACTCACA+AGG - chr2_3:32280131-32280150 None:intergenic 50.0%
!! ATCCACATCCATCGAGTTGG+GGG - chr2_3:32280145-32280164 None:intergenic 50.0%
! CATCCACATCCATCGAGTTG+GGG - chr2_3:32280146-32280165 None:intergenic 50.0%
GACATCCACATCCATCGAGT+TGG - chr2_3:32280148-32280167 None:intergenic 50.0%
CTCGATGGATGTGGATGTCT+TGG + chr2_3:32280149-32280168 Msa0288180:CDS 50.0%
TGTTTGACCCATGCTCGACT+GGG - chr2_3:32280316-32280335 None:intergenic 50.0%
TTGTTTGACCCATGCTCGAC+TGG - chr2_3:32280317-32280336 None:intergenic 50.0%
CCTTGCCTCATGATGACAAC+AGG - chr2_3:32280342-32280361 None:intergenic 50.0%
CCTGTTGTCATCATGAGGCA+AGG + chr2_3:32280339-32280358 Msa0288180:CDS 50.0%
GTTACACCTAGGACTTGGCA+AGG + chr2_3:32280381-32280400 Msa0288180:CDS 50.0%
TTGGCAAGGCAGAGGTTTAC+TGG + chr2_3:32280395-32280414 Msa0288180:CDS 50.0%
TGGCAAGGCAGAGGTTTACT+GGG + chr2_3:32280396-32280415 Msa0288180:CDS 50.0%
! GTATTGGGCTTGGGTTAGAC+TGG - chr2_3:32280551-32280570 None:intergenic 50.0%
!! GATCATCGGAGTATTGGGCT+TGG - chr2_3:32280561-32280580 None:intergenic 50.0%
TGTCTACAGTTGTGGCCGAA+TGG + chr2_3:32280893-32280912 Msa0288180:intron 50.0%
TTAACAGCCCAACTGCTCTC+AGG + chr2_3:32281033-32281052 Msa0288180:intron 50.0%
!! AGAAGTGCCTGAGAGCAGTT+GGG - chr2_3:32281043-32281062 None:intergenic 50.0%
!! AAGAAGTGCCTGAGAGCAGT+TGG - chr2_3:32281044-32281063 None:intergenic 50.0%
! CTGCTCTCAGGCACTTCTTT+TGG + chr2_3:32281045-32281064 Msa0288180:intron 50.0%
!! ACTAAGTGGGGTTGGCTACA+TGG - chr2_3:32281883-32281902 None:intergenic 50.0%
ATGTAGCCAACCCCACTTAG+TGG + chr2_3:32281882-32281901 Msa0288180:intron 50.0%
TGTAGCCAACCCCACTTAGT+GGG + chr2_3:32281883-32281902 Msa0288180:intron 50.0%
CTTATCCCACTAAGTGGGGT+TGG - chr2_3:32281891-32281910 None:intergenic 50.0%
CAACCCCACTTAGTGGGATA+AGG + chr2_3:32281889-32281908 Msa0288180:intron 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - chr2_3:32281897-32281916 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG + chr2_3:32281894-32281913 Msa0288180:intron 50.0%
GGAACGTCGATATCGGAACG+AGG - chr2_3:32279424-32279443 None:intergenic 55.0%
CGTCGTTCAGCTCGAACTAG+TGG - chr2_3:32279445-32279464 None:intergenic 55.0%
!! TATCGGTGTCGGCACATCTG+TGG - chr2_3:32279624-32279643 None:intergenic 55.0%
GAGTCTTTCCCCCAACTCGA+TGG + chr2_3:32280134-32280153 Msa0288180:CDS 55.0%
TTCCCCCAACTCGATGGATG+TGG + chr2_3:32280140-32280159 Msa0288180:CDS 55.0%
GCATCACCAGCGGTAGAAGA+TGG + chr2_3:32280210-32280229 Msa0288180:CDS 55.0%
GGCAAGGCAGAGGTTTACTG+GGG + chr2_3:32280397-32280416 Msa0288180:CDS 55.0%
!! ACAGGTGTCGGTGTCGGACA+CGG + chr2_3:32279678-32279697 Msa0288180:intron 60.0%
!! GCTCCATAGGTTGGCTTCCG+AGG + chr2_3:32280290-32280309 Msa0288180:CDS 60.0%
CCTCTGCCTTGCCAAGTCCT+AGG - chr2_3:32280390-32280409 None:intergenic 60.0%
CCTAGGACTTGGCAAGGCAG+AGG + chr2_3:32280387-32280406 Msa0288180:CDS 60.0%
TCTCAATTCCCTGGCGCCCC+TGG + chr2_3:32279270-32279289 Msa0288180:CDS 65.0%
!! CAGGTGTCGGTGTCGGACAC+GGG + chr2_3:32279679-32279698 Msa0288180:intron 65.0%
GGGCCTCGGAAGCCAACCTA+TGG - chr2_3:32280296-32280315 None:intergenic 65.0%
TTCCGAGGCCCAGTCGAGCA+TGG + chr2_3:32280305-32280324 Msa0288180:CDS 65.0%
TCCGAGGCCCAGTCGAGCAT+GGG + chr2_3:32280306-32280325 Msa0288180:CDS 65.0%
ACCCATGCTCGACTGGGCCT+CGG - chr2_3:32280310-32280329 None:intergenic 65.0%
!! CGGAAAGTCCAGGGGCGCCA+GGG - chr2_3:32279281-32279300 None:intergenic 70.0%
CCTGGCGCCCCTGGACTTTC+CGG + chr2_3:32279279-32279298 Msa0288180:CDS 70.0%
!! CCGGAAAGTCCAGGGGCGCC+AGG - chr2_3:32279282-32279301 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 32279066 32282717 32279066 ID=Msa0288180;Name=Msa0288180
chr2_3 mRNA 32279066 32282717 32279066 ID=Msa0288180-mRNA-1;Parent=Msa0288180;Name=Msa0288180-mRNA-1;_AED=0.21;_eAED=0.21;_QI=111|0.33|0.75|1|0.33|0.75|4|251|178
chr2_3 exon 32279066 32279320 32279066 ID=Msa0288180-mRNA-1:exon:10805;Parent=Msa0288180-mRNA-1
chr2_3 exon 32279789 32279795 32279789 ID=Msa0288180-mRNA-1:exon:10806;Parent=Msa0288180-mRNA-1
chr2_3 exon 32280055 32280418 32280055 ID=Msa0288180-mRNA-1:exon:10807;Parent=Msa0288180-mRNA-1
chr2_3 exon 32282445 32282717 32282445 ID=Msa0288180-mRNA-1:exon:10808;Parent=Msa0288180-mRNA-1
chr2_3 five_prime_UTR 32279066 32279176 32279066 ID=Msa0288180-mRNA-1:five_prime_utr;Parent=Msa0288180-mRNA-1
chr2_3 CDS 32279177 32279320 32279177 ID=Msa0288180-mRNA-1:cds;Parent=Msa0288180-mRNA-1
chr2_3 CDS 32279789 32279795 32279789 ID=Msa0288180-mRNA-1:cds;Parent=Msa0288180-mRNA-1
chr2_3 CDS 32280055 32280418 32280055 ID=Msa0288180-mRNA-1:cds;Parent=Msa0288180-mRNA-1
chr2_3 CDS 32282445 32282466 32282445 ID=Msa0288180-mRNA-1:cds;Parent=Msa0288180-mRNA-1
chr2_3 three_prime_UTR 32282467 32282717 32282467 ID=Msa0288180-mRNA-1:three_prime_utr;Parent=Msa0288180-mRNA-1
Gene Sequence

>Msa0288180

ATGGTGGGAACGAACGTGAAAGCAGAAACAAGATCGCTAATGGATAAGCGAAGTGCGTTAGAGTCTGAAATGAACTCAATCATCGCTCGTCTTTCTCAATTCCCTGGCGCCCCTGGACTTTCCGGCAATCTAATTGATTCTGAGGAAATAGACAATGATGATGGCTCAGACATGCAGACCTCATCTACTGGAGAGACTGTTGCATCAACACCATTGCAGAATGTCCTTGTGAGTCTTTCCCCCAACTCGATGGATGTGGATGTCTTGGTTAGTAGACCTTTTGCAGTGGTTGACGAGATATCAGATGCATCACCAGCGGTAGAAGATGGTTTACAGCTTGGAGATCAAATTTTGAAATTTGGCAACGTCGAAGCAGGTGAAAATTTGCTCCATAGGTTGGCTTCCGAGGCCCAGTCGAGCATGGGTCAAACAATACCTGTTGTCATCATGAGGCAAGGCACAGTGATCAACTTAACTGTTACACCTAGGACTTGGCAAGGCAGAGGTTTACTGGGATGCCATTTTCGGATCTTGTAG
Protein sequence

>Msa0288180

MVGTNVKAETRSLMDKRSALESEMNSIIARLSQFPGAPGLSGNLIDSEEIDNDDGSDMQTSSTGETVASTPLQNVLVSLSPNSMDVDVLVSRPFAVVDEISDASPAVEDGLQLGDQILKFGNVEAGENLLHRLASEAQSSMGQTIPVVIMRQGTVINLTVTPRTWQGRGLLGCHFRIL*