AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1451560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1451560 MtrunA17_Chr8g0360281 97.436 117 3 0 1 117 410 526 4.46e-81 246
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1451560 AT3G15180.1 58.974 117 46 2 1 117 405 519 3.04e-39 137
Msa1451560 AT3G15180.2 58.974 117 46 2 1 117 437 551 5.05e-39 137

Find 25 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTTATGAATATCATTT+TGG 0.264813 tig0033459:-5656 Msa1451560:three_prime_UTR
TAATAGTTTGTTATGACTTC+AGG 0.296563 tig0033459:-5792 Msa1451560:three_prime_UTR
AGTCATAACAAACTATTATT+AGG 0.330833 tig0033459:+5797 None:intergenic
TTAAATGTCTCACCGCTATA+CGG 0.368585 tig0033459:+6989 None:intergenic
GTCTCGACCGTGGTGCTTGA+TGG 0.390375 tig0033459:-6395 Msa1451560:CDS
TTGATCTAGACTTGAGCGTT+TGG 0.409997 tig0033459:-5732 Msa1451560:three_prime_UTR
CTATCAGCCGTCATCACAAT+AGG 0.428327 tig0033459:+5897 None:intergenic
CTATTATTAGGTAAAGAGTA+AGG 0.459022 tig0033459:+5809 None:intergenic
GCAGCTGTTCCTGCAAAAGC+AGG 0.484395 tig0033459:+6107 None:intergenic
GTCTAGATCAAAAGTTGTAA+AGG 0.490892 tig0033459:+5744 None:intergenic
GTTTGTTATGACTTCAGGAT+TGG 0.491099 tig0033459:-5787 Msa1451560:three_prime_UTR
CAGGGTTATAGAATGATAAG+TGG 0.493664 tig0033459:-6424 Msa1451560:CDS
TAAGACTTAAATTGGTACAT+TGG 0.501633 tig0033459:-5687 Msa1451560:three_prime_UTR
CTGCAAGAAGCCGTAGGAAT+GGG 0.511865 tig0033459:-5957 Msa1451560:CDS
CGCTTTCGATGATGAAGATA+CGG 0.525676 tig0033459:+5933 None:intergenic
GCTGCAAGAAGCCGTAGGAA+TGG 0.539955 tig0033459:-5958 Msa1451560:CDS
TGTAGTTTCAGTACTTGGGT+CGG 0.550899 tig0033459:+6336 None:intergenic
TTATAGAATGATAAGTGGAT+TGG 0.557998 tig0033459:-6419 Msa1451560:CDS
AGCTCAACCTATTGTGATGA+CGG 0.603732 tig0033459:-5904 Msa1451560:CDS
AAGATACGGACCCATTCCTA+CGG 0.635459 tig0033459:+5947 None:intergenic
CTAGATATAACTGCTGCATG+CGG 0.647515 tig0033459:-6172 Msa1451560:CDS
GTGCAGCTGCAAGAAGCCGT+AGG 0.664105 tig0033459:-5963 Msa1451560:intron
AGATTCAGAAATCCGTATAG+CGG 0.690364 tig0033459:-7001 Msa1451560:intron
CATATCTCCATCAAGCACCA+CGG 0.693939 tig0033459:+6388 None:intergenic
GTGGATTGGTGTCTCGACCG+TGG 0.710959 tig0033459:-6405 Msa1451560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAATAAAATAGTAATATT+CGG + tig0033459:6437-6456 None:intergenic 10.0%
!!! TTTTTTTTTAAGACTTAAAT+TGG - tig0033459:6983-7002 Msa1451560:intron 10.0%
!!! TTAATTTATGAATATCATTT+TGG - tig0033459:7022-7041 Msa1451560:CDS 10.0%
!! GAAAATAAAATAGTAATATT+CGG + tig0033459:6437-6456 None:intergenic 10.0%
!!! TTTTTTTTTAAGACTTAAAT+TGG - tig0033459:6983-7002 Msa1451560:intron 10.0%
!!! TTAATTTATGAATATCATTT+TGG - tig0033459:7022-7041 Msa1451560:CDS 10.0%
!!! CTTAATTTGATGATATTATT+GGG + tig0033459:5895-5914 None:intergenic 15.0%
!!! TGTATTATTTTAACACAAAT+CGG - tig0033459:6464-6483 Msa1451560:intron 15.0%
!!! CTTAATTTGATGATATTATT+GGG + tig0033459:5895-5914 None:intergenic 15.0%
!!! TGTATTATTTTAACACAAAT+CGG - tig0033459:6464-6483 Msa1451560:intron 15.0%
!! GTAATTTCAATTTCATATGA+TGG - tig0033459:5808-5827 Msa1451560:three_prime_UTR 20.0%
!!! CCTTAATTTGATGATATTAT+TGG + tig0033459:5896-5915 None:intergenic 20.0%
!! CCAATAATATCATCAAATTA+AGG - tig0033459:5893-5912 Msa1451560:CDS 20.0%
!! TAATATCATCAAATTAAGGA+AGG - tig0033459:5897-5916 Msa1451560:CDS 20.0%
!!! ATAGGTAAAAAACTTTTTGT+AGG + tig0033459:6004-6023 None:intergenic 20.0%
!!! ATACTAGATTTTCTAATATC+AGG - tig0033459:6118-6137 Msa1451560:CDS 20.0%
!! AGTCATAACAAACTATTATT+AGG + tig0033459:6884-6903 None:intergenic 20.0%
!! GTAATTTCAATTTCATATGA+TGG - tig0033459:5808-5827 Msa1451560:three_prime_UTR 20.0%
!!! CCTTAATTTGATGATATTAT+TGG + tig0033459:5896-5915 None:intergenic 20.0%
!! CCAATAATATCATCAAATTA+AGG - tig0033459:5893-5912 Msa1451560:CDS 20.0%
!! TAATATCATCAAATTAAGGA+AGG - tig0033459:5897-5916 Msa1451560:CDS 20.0%
!!! ATAGGTAAAAAACTTTTTGT+AGG + tig0033459:6004-6023 None:intergenic 20.0%
!!! ATACTAGATTTTCTAATATC+AGG - tig0033459:6118-6137 Msa1451560:CDS 20.0%
!! AGTCATAACAAACTATTATT+AGG + tig0033459:6884-6903 None:intergenic 20.0%
! TATAAAGCTATGTTATGTTC+AGG + tig0033459:5773-5792 None:intergenic 25.0%
!! ATGCTACATAATTGAGTTTT+GGG + tig0033459:5858-5877 None:intergenic 25.0%
!! AATGCTACATAATTGAGTTT+TGG + tig0033459:5859-5878 None:intergenic 25.0%
! TGCAAAGTTATATTACTAGT+AGG - tig0033459:5930-5949 Msa1451560:CDS 25.0%
! GATGATGTCTAAGAAAAAAT+AGG + tig0033459:6022-6041 None:intergenic 25.0%
!! TTCCTGAATTTTCTTTACAA+CGG + tig0033459:6144-6163 None:intergenic 25.0%
! GACTTTCTTTAGTGAATTTA+AGG + tig0033459:6207-6226 None:intergenic 25.0%
!! TTTTTGTGTGACATATTTTC+AGG - tig0033459:6235-6254 Msa1451560:intron 25.0%
!! TTTTGTGTGACATATTTTCA+GGG - tig0033459:6236-6255 Msa1451560:intron 25.0%
! TTATAGAATGATAAGTGGAT+TGG - tig0033459:6259-6278 Msa1451560:intron 25.0%
!! TTTTTGTAGTTTCAGTACTT+GGG + tig0033459:6349-6368 None:intergenic 25.0%
!! ATTTTTGTAGTTTCAGTACT+TGG + tig0033459:6350-6369 None:intergenic 25.0%
!! AGTACTGAAACTACAAAAAT+AGG - tig0033459:6350-6369 Msa1451560:CDS 25.0%
!!! ACTGTTTTCTTGATTTGTTT+AGG + tig0033459:6656-6675 None:intergenic 25.0%
! CTATTATTAGGTAAAGAGTA+AGG + tig0033459:6872-6891 None:intergenic 25.0%
!!! TAATAGTTTGTTATGACTTC+AGG - tig0033459:6886-6905 Msa1451560:intron 25.0%
! TAAGACTTAAATTGGTACAT+TGG - tig0033459:6991-7010 Msa1451560:intron 25.0%
! TATAAAGCTATGTTATGTTC+AGG + tig0033459:5773-5792 None:intergenic 25.0%
!! ATGCTACATAATTGAGTTTT+GGG + tig0033459:5858-5877 None:intergenic 25.0%
!! AATGCTACATAATTGAGTTT+TGG + tig0033459:5859-5878 None:intergenic 25.0%
! TGCAAAGTTATATTACTAGT+AGG - tig0033459:5930-5949 Msa1451560:CDS 25.0%
! GATGATGTCTAAGAAAAAAT+AGG + tig0033459:6022-6041 None:intergenic 25.0%
!! TTCCTGAATTTTCTTTACAA+CGG + tig0033459:6144-6163 None:intergenic 25.0%
! GACTTTCTTTAGTGAATTTA+AGG + tig0033459:6207-6226 None:intergenic 25.0%
!! TTTTTGTGTGACATATTTTC+AGG - tig0033459:6235-6254 Msa1451560:intron 25.0%
!! TTTTGTGTGACATATTTTCA+GGG - tig0033459:6236-6255 Msa1451560:intron 25.0%
! TTATAGAATGATAAGTGGAT+TGG - tig0033459:6259-6278 Msa1451560:intron 25.0%
!! TTTTTGTAGTTTCAGTACTT+GGG + tig0033459:6349-6368 None:intergenic 25.0%
!! ATTTTTGTAGTTTCAGTACT+TGG + tig0033459:6350-6369 None:intergenic 25.0%
!! AGTACTGAAACTACAAAAAT+AGG - tig0033459:6350-6369 Msa1451560:CDS 25.0%
!!! ACTGTTTTCTTGATTTGTTT+AGG + tig0033459:6656-6675 None:intergenic 25.0%
! CTATTATTAGGTAAAGAGTA+AGG + tig0033459:6872-6891 None:intergenic 25.0%
!!! TAATAGTTTGTTATGACTTC+AGG - tig0033459:6886-6905 Msa1451560:intron 25.0%
! TAAGACTTAAATTGGTACAT+TGG - tig0033459:6991-7010 Msa1451560:intron 25.0%
! CATATGATGGAAATGAAATC+AGG - tig0033459:5821-5840 Msa1451560:three_prime_UTR 30.0%
TTTCTTAGACATCATCATAG+AGG - tig0033459:6026-6045 Msa1451560:intron 30.0%
TCTTTAGTGAATTTAAGGAC+AGG + tig0033459:6202-6221 None:intergenic 30.0%
AAACACAAGCAATGCATAAT+GGG + tig0033459:6618-6637 None:intergenic 30.0%
AGTGTAAATGCTAAACTTTC+CGG - tig0033459:6673-6692 Msa1451560:intron 30.0%
! TGTAATTTTGCAATTTGCAG+TGG - tig0033459:6833-6852 Msa1451560:intron 30.0%
GTCTAGATCAAAAGTTGTAA+AGG + tig0033459:6937-6956 None:intergenic 30.0%
!! ATGAATATCATTTTGGTCAC+AGG - tig0033459:7029-7048 Msa1451560:CDS 30.0%
! CATATGATGGAAATGAAATC+AGG - tig0033459:5821-5840 Msa1451560:three_prime_UTR 30.0%
TTTCTTAGACATCATCATAG+AGG - tig0033459:6026-6045 Msa1451560:intron 30.0%
TCTTTAGTGAATTTAAGGAC+AGG + tig0033459:6202-6221 None:intergenic 30.0%
AAACACAAGCAATGCATAAT+GGG + tig0033459:6618-6637 None:intergenic 30.0%
AGTGTAAATGCTAAACTTTC+CGG - tig0033459:6673-6692 Msa1451560:intron 30.0%
! TGTAATTTTGCAATTTGCAG+TGG - tig0033459:6833-6852 Msa1451560:intron 30.0%
GTCTAGATCAAAAGTTGTAA+AGG + tig0033459:6937-6956 None:intergenic 30.0%
AGATTCAGAAATCCGTATAG+CGG - tig0033459:5677-5696 Msa1451560:three_prime_UTR 35.0%
TTAAATGTCTCACCGCTATA+CGG + tig0033459:5692-5711 None:intergenic 35.0%
CAGGGTTATAGAATGATAAG+TGG - tig0033459:6254-6273 Msa1451560:intron 35.0%
ATATTCGGACTGTAAACATG+TGG + tig0033459:6422-6441 None:intergenic 35.0%
! AACACAAATCGGAGTATTTC+AGG - tig0033459:6475-6494 Msa1451560:intron 35.0%
! AAATCGGAGTATTTCAGGAA+TGG - tig0033459:6480-6499 Msa1451560:intron 35.0%
! CAATGCATAATGGGAAAGTT+TGG + tig0033459:6609-6628 None:intergenic 35.0%
CAAACACAAGCAATGCATAA+TGG + tig0033459:6619-6638 None:intergenic 35.0%
ACAAGATACACAAACACAAC+CGG + tig0033459:6695-6714 None:intergenic 35.0%
!! GTTTGTTATGACTTCAGGAT+TGG - tig0033459:6891-6910 Msa1451560:intron 35.0%
AGATTCAGAAATCCGTATAG+CGG - tig0033459:5677-5696 Msa1451560:three_prime_UTR 35.0%
TTAAATGTCTCACCGCTATA+CGG + tig0033459:5692-5711 None:intergenic 35.0%
CAGGGTTATAGAATGATAAG+TGG - tig0033459:6254-6273 Msa1451560:intron 35.0%
ATATTCGGACTGTAAACATG+TGG + tig0033459:6422-6441 None:intergenic 35.0%
! AACACAAATCGGAGTATTTC+AGG - tig0033459:6475-6494 Msa1451560:intron 35.0%
! AAATCGGAGTATTTCAGGAA+TGG - tig0033459:6480-6499 Msa1451560:intron 35.0%
! CAATGCATAATGGGAAAGTT+TGG + tig0033459:6609-6628 None:intergenic 35.0%
CAAACACAAGCAATGCATAA+TGG + tig0033459:6619-6638 None:intergenic 35.0%
ACAAGATACACAAACACAAC+CGG + tig0033459:6695-6714 None:intergenic 35.0%
!! GTTTGTTATGACTTCAGGAT+TGG - tig0033459:6891-6910 Msa1451560:intron 35.0%
! AGTCTTGTATTTGCAGCAAG+AGG + tig0033459:5980-5999 None:intergenic 40.0%
GGCCGTTGTAAAGAAAATTC+AGG - tig0033459:6139-6158 Msa1451560:CDS 40.0%
TGTAGTTTCAGTACTTGGGT+CGG + tig0033459:6345-6364 None:intergenic 40.0%
CTAGATATAACTGCTGCATG+CGG - tig0033459:6506-6525 Msa1451560:intron 40.0%
CGCTTTCGATGATGAAGATA+CGG + tig0033459:6748-6767 None:intergenic 40.0%
AGCTCAACCTATTGTGATGA+CGG - tig0033459:6774-6793 Msa1451560:intron 40.0%
!!! GATTTTAGTTTGCACAGCAC+AGG - tig0033459:6803-6822 Msa1451560:intron 40.0%
! TTTTGCAATTTGCAGTGGTG+TGG - tig0033459:6838-6857 Msa1451560:intron 40.0%
TTGATCTAGACTTGAGCGTT+TGG - tig0033459:6946-6965 Msa1451560:intron 40.0%
! AGTCTTGTATTTGCAGCAAG+AGG + tig0033459:5980-5999 None:intergenic 40.0%
GGCCGTTGTAAAGAAAATTC+AGG - tig0033459:6139-6158 Msa1451560:CDS 40.0%
TGTAGTTTCAGTACTTGGGT+CGG + tig0033459:6345-6364 None:intergenic 40.0%
CTAGATATAACTGCTGCATG+CGG - tig0033459:6506-6525 Msa1451560:intron 40.0%
CGCTTTCGATGATGAAGATA+CGG + tig0033459:6748-6767 None:intergenic 40.0%
AGCTCAACCTATTGTGATGA+CGG - tig0033459:6774-6793 Msa1451560:intron 40.0%
!!! GATTTTAGTTTGCACAGCAC+AGG - tig0033459:6803-6822 Msa1451560:intron 40.0%
! TTTTGCAATTTGCAGTGGTG+TGG - tig0033459:6838-6857 Msa1451560:intron 40.0%
TTGATCTAGACTTGAGCGTT+TGG - tig0033459:6946-6965 Msa1451560:intron 40.0%
GCAGCAAGAGGAAGATTCTT+AGG + tig0033459:5968-5987 None:intergenic 45.0%
CATATCTCCATCAAGCACCA+CGG + tig0033459:6293-6312 None:intergenic 45.0%
CGGACTGTAAACATGTGGAA+TGG + tig0033459:6417-6436 None:intergenic 45.0%
! TTTTGCAGGAACAGCTGCAA+AGG - tig0033459:6576-6595 Msa1451560:intron 45.0%
AAGATACGGACCCATTCCTA+CGG + tig0033459:6734-6753 None:intergenic 45.0%
CTATCAGCCGTCATCACAAT+AGG + tig0033459:6784-6803 None:intergenic 45.0%
GCAGCAAGAGGAAGATTCTT+AGG + tig0033459:5968-5987 None:intergenic 45.0%
CATATCTCCATCAAGCACCA+CGG + tig0033459:6293-6312 None:intergenic 45.0%
CGGACTGTAAACATGTGGAA+TGG + tig0033459:6417-6436 None:intergenic 45.0%
! TTTTGCAGGAACAGCTGCAA+AGG - tig0033459:6576-6595 Msa1451560:intron 45.0%
AAGATACGGACCCATTCCTA+CGG + tig0033459:6734-6753 None:intergenic 45.0%
CTATCAGCCGTCATCACAAT+AGG + tig0033459:6784-6803 None:intergenic 45.0%
!! TCTGCTGATCCTGCTTTTGC+AGG - tig0033459:6562-6581 Msa1451560:intron 50.0%
CTGCAAGAAGCCGTAGGAAT+GGG - tig0033459:6721-6740 Msa1451560:intron 50.0%
!! TCTGCTGATCCTGCTTTTGC+AGG - tig0033459:6562-6581 Msa1451560:intron 50.0%
CTGCAAGAAGCCGTAGGAAT+GGG - tig0033459:6721-6740 Msa1451560:intron 50.0%
GCAGCTGTTCCTGCAAAAGC+AGG + tig0033459:6574-6593 None:intergenic 55.0%
GCTGCAAGAAGCCGTAGGAA+TGG - tig0033459:6720-6739 Msa1451560:intron 55.0%
GCAGCTGTTCCTGCAAAAGC+AGG + tig0033459:6574-6593 None:intergenic 55.0%
GCTGCAAGAAGCCGTAGGAA+TGG - tig0033459:6720-6739 Msa1451560:intron 55.0%
!! GTGGATTGGTGTCTCGACCG+TGG - tig0033459:6273-6292 Msa1451560:intron 60.0%
GTCTCGACCGTGGTGCTTGA+TGG - tig0033459:6283-6302 Msa1451560:intron 60.0%
GTGCAGCTGCAAGAAGCCGT+AGG - tig0033459:6715-6734 Msa1451560:intron 60.0%
!! GTGGATTGGTGTCTCGACCG+TGG - tig0033459:6273-6292 Msa1451560:intron 60.0%
GTCTCGACCGTGGTGCTTGA+TGG - tig0033459:6283-6302 Msa1451560:intron 60.0%
GTGCAGCTGCAAGAAGCCGT+AGG - tig0033459:6715-6734 Msa1451560:intron 60.0%
Chromosome Type Strat End Strand Name
tig0033459 gene 5651 7049 5651 ID=Msa1451560;Name=Msa1451560
tig0033459 mRNA 5651 7049 5651 ID=Msa1451560-mRNA-1;Parent=Msa1451560;Name=Msa1451560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.75|1|1|1|4|239|117
tig0033459 exon 5651 5979 5651 ID=Msa1451560-mRNA-1:exon:18105;Parent=Msa1451560-mRNA-1
tig0033459 exon 6103 6203 6103 ID=Msa1451560-mRNA-1:exon:18104;Parent=Msa1451560-mRNA-1
tig0033459 exon 6329 6443 6329 ID=Msa1451560-mRNA-1:exon:18103;Parent=Msa1451560-mRNA-1
tig0033459 exon 7002 7049 7002 ID=Msa1451560-mRNA-1:exon:18102;Parent=Msa1451560-mRNA-1
tig0033459 CDS 7002 7049 7002 ID=Msa1451560-mRNA-1:cds;Parent=Msa1451560-mRNA-1
tig0033459 CDS 6329 6443 6329 ID=Msa1451560-mRNA-1:cds;Parent=Msa1451560-mRNA-1
tig0033459 CDS 6103 6203 6103 ID=Msa1451560-mRNA-1:cds;Parent=Msa1451560-mRNA-1
tig0033459 CDS 5890 5979 5890 ID=Msa1451560-mRNA-1:cds;Parent=Msa1451560-mRNA-1
tig0033459 three_prime_UTR 5651 5889 5651 ID=Msa1451560-mRNA-1:three_prime_utr;Parent=Msa1451560-mRNA-1
Gene Sequence

>Msa1451560

GGTCTATTTCTATCAGTTTTGCAGCAAGATTCAGAAATCCGTATAGCGGGTTATAGAATGATAAGTGGATTGGTGTCTCGACCGTGGTGCTTGATGGAGATATGCTCAAGACAAGAGATTATAAATATTGTGACCGACCCAAGTACTGAAACTACAAAAATAGGAATGGAAGCTAGATATAACTGCTGCATGCGGATAAACAAATCACTGACACAGTCAAGTAGAGTCTCTGCTGATCCTGCTTTTGCAGGAACAGCTGCAAAGCTGCAAGAAGCCGTAGGAATGGGTCCGTATCTTCATCATCGAAAGCGTGTTGAAGCTCAACCTATTGTGATGACGGCTGATAGATTTTAG

Protein sequence

>Msa1451560

GLFLSVLQQDSEIRIAGYRMISGLVSRPWCLMEICSRQEIINIVTDPSTETTKIGMEARYNCCMRINKSLTQSSRVSADPAFAGTAAKLQEAVGMGPYLHHRKRVEAQPIVMTADRF*