AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0302460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302460 MtrunA17_Chr2g0328131 96.599 147 5 0 1 147 1 147 4.79e-101 286
Msa0302460 MtrunA17_Chr2g0328141 86.014 143 20 0 1 143 1 143 4.44e-76 223
Msa0302460 MtrunA17_Chr4g0068231 33.766 154 75 2 18 144 24 177 3.25e-22 87.0
Msa0302460 MtrunA17_Chr2g0294641 33.548 155 75 3 18 144 26 180 2.41e-20 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302460 AT2G28900.1 61.224 147 57 0 1 147 1 147 5.30e-62 187
Msa0302460 AT4G16160.1 32.026 153 77 3 18 143 21 173 4.33e-19 79.3
Msa0302460 AT4G16160.2 31.613 155 77 3 18 143 21 175 6.03e-19 79.0

Find 123 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGATAATGAAGGAATTT+TGG 0.142163 2_3:+59238976 Msa0302460:three_prime_UTR
TTCTGTCAATTCTTGGTCTT+TGG 0.203392 2_3:+59238397 Msa0302460:three_prime_UTR
CCTTTGCCAATGTGAGTCTT+AGG 0.217782 2_3:-59239134 None:intergenic
ATGCCTCATAGCAGCATTTC+AGG 0.220503 2_3:+59236682 Msa0302460:CDS
TTCAGCTTTCTGTCAATTCT+TGG 0.245659 2_3:+59238390 Msa0302460:three_prime_UTR
CTTCTCAACCTCACTGTTGA+TGG 0.269257 2_3:+59236754 Msa0302460:CDS
CACAAAAGTGTGACCACTTA+TGG 0.284275 2_3:-59236542 None:intergenic
CATCCAAACTCGTTCCAAAA+AGG 0.284341 2_3:+59239184 Msa0302460:three_prime_UTR
GCTGGCTGCTGAGTGGTTAC+TGG 0.311741 2_3:-59238943 None:intergenic
TTGAAAAGGGGTTGCACATT+TGG 0.315261 2_3:+59238303 Msa0302460:three_prime_UTR
AGTCTGGGCAAAAGAGTTAT+AGG 0.327469 2_3:+59238903 Msa0302460:three_prime_UTR
TCTAAGCAAGCATCCTCTTT+AGG 0.329719 2_3:-59238690 None:intergenic
ATTGTAGTAGGTGCCATTAC+AGG 0.330295 2_3:+59238195 Msa0302460:CDS
ACTGCTGCAGCATTCATAAA+TGG 0.345573 2_3:+59238231 Msa0302460:CDS
GGAACAGCCAAAGACAAAAC+TGG 0.362261 2_3:-59238855 None:intergenic
CATTTAAAGAAGGTGCATAC+TGG 0.364036 2_3:+59237646 Msa0302460:CDS
TAAATGGCTGGAGTTTCTAA+TGG 0.368333 2_3:+59238247 Msa0302460:CDS
CATCAAAATTATTACATAAC+CGG 0.374833 2_3:-59238344 None:intergenic
ATATATATCATGATTTCAAC+AGG 0.380020 2_3:-59238580 None:intergenic
CTATTATTCCATGTTTGAAA+AGG 0.387461 2_3:+59238289 Msa0302460:three_prime_UTR
GTTGATGGTTTCTTGAAGAT+TGG 0.388016 2_3:+59236769 Msa0302460:CDS
CTTAGTCTTCCTACCAGGCT+TGG 0.394054 2_3:-59239099 None:intergenic
TGTGGATGAAGATTTGAGTC+TGG 0.401407 2_3:+59238887 Msa0302460:three_prime_UTR
CTCAGACATTAACACTGATA+TGG 0.403641 2_3:+59239025 Msa0302460:three_prime_UTR
CAAATGTTGGACTCTGTCTC+GGG 0.404366 2_3:+59238789 Msa0302460:three_prime_UTR
AGATGCTATGTCAGTATGTT+TGG 0.419644 2_3:-59239072 None:intergenic
AGCTACCAGCTTGAATGTTA+AGG 0.419870 2_3:-59236614 None:intergenic
TATTATTCCATGTTTGAAAA+GGG 0.422205 2_3:+59238290 Msa0302460:three_prime_UTR
GGATCCGTGGCACCAGAGAC+TGG 0.423846 2_3:+59237805 Msa0302460:CDS
ACATTTGGAGGTGCAGTGAC+AGG 0.426302 2_3:+59238132 Msa0302460:CDS
TTGGACTCTGTCTCGGGGAG+GGG 0.430301 2_3:+59238795 Msa0302460:three_prime_UTR
GAACACCGAACCACCAAACT+TGG 0.430785 2_3:-59238825 None:intergenic
GTATATGTTGGAACAGAATA+CGG 0.434501 2_3:+59237774 Msa0302460:CDS
CTTTGCCAATGTGAGTCTTA+GGG 0.439223 2_3:-59239133 None:intergenic
CGCAATTGCAGCCCCTGTAA+TGG 0.440225 2_3:-59238208 None:intergenic
AGGTGCAGTGACAGGGGCTC+TGG 0.442203 2_3:+59238140 Msa0302460:CDS
TGCTTCTTAGTCTTCCTACC+AGG 0.442253 2_3:-59239104 None:intergenic
AGAGTTATAGGTGGAAAGTC+TGG 0.443810 2_3:+59238915 Msa0302460:three_prime_UTR
CACCTATTCCATTGGAATCA+AGG 0.445195 2_3:+59238733 Msa0302460:three_prime_UTR
GATGAAGGGGACGTGTTGTA+AGG 0.446624 2_3:+59238545 Msa0302460:three_prime_UTR
GTTGGACTCTGTCTCGGGGA+GGG 0.450789 2_3:+59238794 Msa0302460:three_prime_UTR
TGGACTCTGTCTCGGGGAGG+GGG 0.451579 2_3:+59238796 Msa0302460:three_prime_UTR
AAAGAAACCTGGGCATGAAC+AGG 0.452707 2_3:+59238444 Msa0302460:three_prime_UTR
GTGATCCAAGTTTGGTGGTT+CGG 0.457160 2_3:+59238820 Msa0302460:three_prime_UTR
GCAAATGTTGGACTCTGTCT+CGG 0.470535 2_3:+59238788 Msa0302460:three_prime_UTR
ATTTGCTTGTTAGAAACTAT+AGG 0.472776 2_3:-59238771 None:intergenic
TTTCTGTGTATATCCTAAAG+AGG 0.475750 2_3:+59238677 Msa0302460:three_prime_UTR
GGCCTTGATTCCAATGGAAT+AGG 0.478081 2_3:-59238735 None:intergenic
ACCCTTTCGAGCAATATGAT+AGG 0.487140 2_3:-59236914 None:intergenic
TGAGAGAATTACCCAGTCTC+TGG 0.488901 2_3:-59237817 None:intergenic
GTCAATGACCACATCGACCT+TGG 0.493655 2_3:-59236719 None:intergenic
ATTTAAAGAAGGTGCATACT+GGG 0.494694 2_3:+59237647 Msa0302460:CDS
ACCTATCATATTGCTCGAAA+GGG 0.494769 2_3:+59236913 Msa0302460:CDS
AAGGCAGACAAGATTGTAGT+AGG 0.498217 2_3:+59238183 Msa0302460:CDS
AAGGGGACGTGTTGTAAGGT+AGG 0.499858 2_3:+59238549 Msa0302460:three_prime_UTR
GCTATAGCTGGTGTATATGT+TGG 0.502697 2_3:+59237762 Msa0302460:CDS
AACGTTAAACTTTATTCACC+TGG 0.506197 2_3:-59238490 None:intergenic
ATCTTCATCCACAGAAGTGA+CGG 0.511130 2_3:-59238877 None:intergenic
CTGCAGCATTCATAAATGGC+TGG 0.512276 2_3:+59238235 Msa0302460:CDS
TACCTATCATATTGCTCGAA+AGG 0.512817 2_3:+59236912 Msa0302460:CDS
AAATTTAAACATTAGCAGCC+AGG 0.512839 2_3:+59238472 Msa0302460:three_prime_UTR
ATCTGCAGCCAACAATGAAA+AGG 0.513448 2_3:+59238164 Msa0302460:CDS
TTGAAAACCTGTTCATGCCC+AGG 0.516629 2_3:-59238451 None:intergenic
TCAATGACCACATCGACCTT+GGG 0.517343 2_3:-59236718 None:intergenic
TTGTAGTAGGTGCCATTACA+GGG 0.523008 2_3:+59238196 Msa0302460:CDS
AGTTTCTAACAAGCAAATGT+TGG 0.526698 2_3:+59238776 Msa0302460:three_prime_UTR
GCAAGATGCACCTATTCCAT+TGG 0.528869 2_3:+59238725 Msa0302460:three_prime_UTR
AGGATCAGTGTCTTCTCCCA+AGG 0.530325 2_3:+59236702 Msa0302460:CDS
TATATGTTGGAACAGAATAC+GGG 0.530549 2_3:+59237775 Msa0302460:CDS
GGCTGTTCCCGTCACTTCTG+TGG 0.535219 2_3:+59238869 Msa0302460:three_prime_UTR
GAACAGCCAAAGACAAAACT+GGG 0.536588 2_3:-59238854 None:intergenic
CACATTGGCAAAGGTTCTGA+TGG 0.538523 2_3:+59239143 Msa0302460:three_prime_UTR
ACCTTCCTTAACATTCAAGC+TGG 0.539605 2_3:+59236609 Msa0302460:five_prime_UTR
GAGGATGCTTGCTTAGAGCC+AGG 0.540490 2_3:+59238696 Msa0302460:three_prime_UTR
CATCTAACACCAAGCCTGGT+AGG 0.540647 2_3:+59239090 Msa0302460:three_prime_UTR
GGTTCTAGATCATGAGATGA+AGG 0.541822 2_3:+59238530 Msa0302460:three_prime_UTR
CATTTGGAGGTGCAGTGACA+GGG 0.546047 2_3:+59238133 Msa0302460:CDS
TTGTTAGAAACTATAGGTAG+AGG 0.547056 2_3:-59238765 None:intergenic
TAAAAGTGTGCAGATAATGA+AGG 0.550725 2_3:+59238968 Msa0302460:three_prime_UTR
ATAGCATCTAACACCAAGCC+TGG 0.555962 2_3:+59239086 Msa0302460:three_prime_UTR
ACGCTTACAGGAGCTATAGC+TGG 0.557663 2_3:+59237750 Msa0302460:intron
ATTATTCCATGTTTGAAAAG+GGG 0.564765 2_3:+59238291 Msa0302460:three_prime_UTR
GGTCGATGTGGTCATTGACA+TGG 0.564917 2_3:+59236723 Msa0302460:CDS
GATCCTGAAATGCTGCTATG+AGG 0.565334 2_3:-59236685 None:intergenic
ATCCTCTGCAAGTGCCCTTG+TGG 0.568133 2_3:-59236890 None:intergenic
GTGGATGAAGATTTGAGTCT+GGG 0.570778 2_3:+59238888 Msa0302460:three_prime_UTR
CTTTCAATCACAGCCATAAG+TGG 0.570799 2_3:+59236529 None:intergenic
ACTATAGGTAGAGGCTGAGA+GGG 0.572049 2_3:-59238756 None:intergenic
AACTATAGGTAGAGGCTGAG+AGG 0.577361 2_3:-59238757 None:intergenic
TGTCACTGCACCTCCAAATG+TGG 0.580744 2_3:-59238130 None:intergenic
TGAGAGGGCCTTGATTCCAA+TGG 0.582673 2_3:-59238741 None:intergenic
TGTTGGACTCTGTCTCGGGG+AGG 0.585063 2_3:+59238793 Msa0302460:three_prime_UTR
GGGGTGTGATCCAAGTTTGG+TGG 0.586053 2_3:+59238815 Msa0302460:three_prime_UTR
CTGAAGAAGACATTTAAAGA+AGG 0.586357 2_3:+59237636 Msa0302460:CDS
TTCTAGATCATGAGATGAAG+GGG 0.587925 2_3:+59238532 Msa0302460:three_prime_UTR
TTACCCAGTCTCTGGTGCCA+CGG 0.593471 2_3:-59237809 None:intergenic
GATCCGTGGCACCAGAGACT+GGG 0.594640 2_3:+59237806 Msa0302460:CDS
AGCTTGAATGTTAAGGAAGG+TGG 0.594953 2_3:-59236607 None:intergenic
AGCCACAAGGGCACTTGCAG+AGG 0.596020 2_3:+59236888 Msa0302460:CDS
CATATCAGTGTTAATGTCTG+AGG 0.597268 2_3:-59239024 None:intergenic
GTTCTAGATCATGAGATGAA+GGG 0.598978 2_3:+59238531 Msa0302460:three_prime_UTR
AAAAGCCCTAAGACTCACAT+TGG 0.608767 2_3:+59239128 Msa0302460:three_prime_UTR
TCTTCATCCACAGAAGTGAC+GGG 0.611855 2_3:-59238876 None:intergenic
TACGGGGTGGAGAGGATCCG+TGG 0.613293 2_3:+59237792 Msa0302460:CDS
ATATACTACTATATAGCAAG+AGG 0.614367 2_3:-59236571 None:intergenic
CCTAAGACTCACATTGGCAA+AGG 0.618293 2_3:+59239134 Msa0302460:three_prime_UTR
GTCTTCTCCCAAGGTCGATG+TGG 0.618468 2_3:+59236711 Msa0302460:CDS
GAACAGAATACGGGGTGGAG+AGG 0.619958 2_3:+59237784 Msa0302460:CDS
GTCGATGTGGTCATTGACAT+GGG 0.620754 2_3:+59236724 Msa0302460:CDS
GGTGCATCTTGCTCTAGACC+TGG 0.627077 2_3:-59238714 None:intergenic
ACCAGCTTGAATGTTAAGGA+AGG 0.629164 2_3:-59236610 None:intergenic
TCAACAGTGAGGTTGAGAAG+TGG 0.636785 2_3:-59236751 None:intergenic
ATTTGGAGGTGCAGTGACAG+GGG 0.640699 2_3:+59238134 Msa0302460:CDS
CTGGGCAAAAGAGTTATAGG+TGG 0.668810 2_3:+59238906 Msa0302460:three_prime_UTR
AAATGTTGGACTCTGTCTCG+GGG 0.672447 2_3:+59238790 Msa0302460:three_prime_UTR
GGTAGCTAAACAAAGCAAAG+AGG 0.681272 2_3:+59236630 Msa0302460:five_prime_UTR
TTTAAAGAAGGTGCATACTG+GGG 0.700970 2_3:+59237648 Msa0302460:CDS
TGTAGTAGGTGCCATTACAG+GGG 0.704939 2_3:+59238197 Msa0302460:CDS
TCAAGAAACCATCAACAGTG+AGG 0.715759 2_3:-59236762 None:intergenic
TGTTGGAACAGAATACGGGG+TGG 0.732754 2_3:+59237779 Msa0302460:CDS
TATATATCATGATTTCAACA+GGG 0.732772 2_3:-59238579 None:intergenic
AACAGCCAAAGACAAAACTG+GGG 0.763097 2_3:-59238853 None:intergenic
ATATGTTGGAACAGAATACG+GGG 0.764667 2_3:+59237776 Msa0302460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTAAAAATTATATTTT+AGG - chr2_3:59237207-59237226 None:intergenic 0.0%
!!! AAATTATATTTTAGGAGTAA+AGG - chr2_3:59237199-59237218 None:intergenic 15.0%
!!! TTTTGGAGATTTTAACAAAA+GGG - chr2_3:59236965-59236984 None:intergenic 20.0%
!! AAAAGATGAAAACTTTGATT+CGG + chr2_3:59236981-59237000 Msa0302460:intron 20.0%
!!! TTATCATGATTGATTCATTT+GGG + chr2_3:59237169-59237188 Msa0302460:intron 20.0%
!! TATTTATCAAAGTTAAAGCA+TGG - chr2_3:59237435-59237454 None:intergenic 20.0%
!!! ATGCTTTAACTTTGATAAAT+AGG + chr2_3:59237434-59237453 Msa0302460:intron 20.0%
!!! AAATGTGTTAACTTTGTTTT+AGG + chr2_3:59238012-59238031 Msa0302460:intron 20.0%
!! AACTTAAATTGAACAATAGA+AGG - chr2_3:59238050-59238069 None:intergenic 20.0%
!! TATTATTCCATGTTTGAAAA+GGG + chr2_3:59238290-59238309 Msa0302460:three_prime_UTR 20.0%
!! CATCAAAATTATTACATAAC+CGG - chr2_3:59238347-59238366 None:intergenic 20.0%
!!! CGTTTTCATAAATATTATTG+CGG + chr2_3:59238509-59238528 Msa0302460:three_prime_UTR 20.0%
!! TATATATCATGATTTCAACA+GGG - chr2_3:59238582-59238601 None:intergenic 20.0%
!! ATATATATCATGATTTCAAC+AGG - chr2_3:59238583-59238602 None:intergenic 20.0%
!!! TTCAGTATTTTTTAAAGCAA+CGG - chr2_3:59238621-59238640 None:intergenic 20.0%
! ATATACTACTATATAGCAAG+AGG - chr2_3:59236574-59236593 None:intergenic 25.0%
!!! CTTTTGGAGATTTTAACAAA+AGG - chr2_3:59236966-59236985 None:intergenic 25.0%
!! GAATCAAAGTTTTCATCTTT+TGG - chr2_3:59236982-59237001 None:intergenic 25.0%
! AAAGATGAAAACTTTGATTC+GGG + chr2_3:59236982-59237001 Msa0302460:intron 25.0%
!! CTTATCATGATTGATTCATT+TGG + chr2_3:59237168-59237187 Msa0302460:intron 25.0%
!! TATCATGATTGATTCATTTG+GGG + chr2_3:59237170-59237189 Msa0302460:intron 25.0%
! CAATAATGTGATTTGAACTT+GGG + chr2_3:59237288-59237307 Msa0302460:intron 25.0%
! AGACAAAATGTCTATTGAAT+GGG - chr2_3:59237571-59237590 None:intergenic 25.0%
! TAGACAAAATGTCTATTGAA+TGG - chr2_3:59237572-59237591 None:intergenic 25.0%
! GAAAACACTTAAAATAGACT+TGG + chr2_3:59237851-59237870 Msa0302460:intron 25.0%
! AGAGATTAGATGTCATTATA+TGG - chr2_3:59237929-59237948 None:intergenic 25.0%
! CTATTATTCCATGTTTGAAA+AGG + chr2_3:59238289-59238308 Msa0302460:three_prime_UTR 25.0%
! ATTATTCCATGTTTGAAAAG+GGG + chr2_3:59238291-59238310 Msa0302460:three_prime_UTR 25.0%
!! CGGTTATGTAATAATTTTGA+TGG + chr2_3:59238345-59238364 Msa0302460:three_prime_UTR 25.0%
!! ACTTTTAGTATAAAGAAACC+TGG + chr2_3:59238433-59238452 Msa0302460:three_prime_UTR 25.0%
!! CTTTTAGTATAAAGAAACCT+GGG + chr2_3:59238434-59238453 Msa0302460:three_prime_UTR 25.0%
!! ATTTGCTTGTTAGAAACTAT+AGG - chr2_3:59238774-59238793 None:intergenic 25.0%
GCAATAATGTGATTTGAACT+TGG + chr2_3:59237287-59237306 Msa0302460:intron 30.0%
TCCTAGAAAAGAATAATGAC+AGG + chr2_3:59237319-59237338 Msa0302460:intron 30.0%
! TCCTGTCATTATTCTTTTCT+AGG - chr2_3:59237323-59237342 None:intergenic 30.0%
AATGAGAGAATCCGAATTTA+GGG - chr2_3:59237350-59237369 None:intergenic 30.0%
AAATGAGAGAATCCGAATTT+AGG - chr2_3:59237351-59237370 None:intergenic 30.0%
TTGAGCTGTTTGATCAAAAT+CGG + chr2_3:59237404-59237423 Msa0302460:intron 30.0%
TGAGAATCTAAATCCTGAAA+AGG + chr2_3:59237537-59237556 Msa0302460:intron 30.0%
GAGAATCTAAATCCTGAAAA+GGG + chr2_3:59237538-59237557 Msa0302460:intron 30.0%
CTGAAGAAGACATTTAAAGA+AGG + chr2_3:59237636-59237655 Msa0302460:CDS 30.0%
! ATTTAAAGAAGGTGCATACT+GGG + chr2_3:59237647-59237666 Msa0302460:CDS 30.0%
GTATATGTTGGAACAGAATA+CGG + chr2_3:59237774-59237793 Msa0302460:CDS 30.0%
TATATGTTGGAACAGAATAC+GGG + chr2_3:59237775-59237794 Msa0302460:CDS 30.0%
AAATTGAACAATAGAAGGCA+TGG - chr2_3:59238045-59238064 None:intergenic 30.0%
AAATTTAAACATTAGCAGCC+AGG + chr2_3:59238472-59238491 Msa0302460:three_prime_UTR 30.0%
AACGTTAAACTTTATTCACC+TGG - chr2_3:59238493-59238512 None:intergenic 30.0%
TTTCTGTGTATATCCTAAAG+AGG + chr2_3:59238677-59238696 Msa0302460:three_prime_UTR 30.0%
TTGTTAGAAACTATAGGTAG+AGG - chr2_3:59238768-59238787 None:intergenic 30.0%
AGTTTCTAACAAGCAAATGT+TGG + chr2_3:59238776-59238795 Msa0302460:three_prime_UTR 30.0%
! TCATTATCTGCACACTTTTA+AGG - chr2_3:59238968-59238987 None:intergenic 30.0%
! TAAAAGTGTGCAGATAATGA+AGG + chr2_3:59238968-59238987 Msa0302460:three_prime_UTR 30.0%
! TGCAGATAATGAAGGAATTT+TGG + chr2_3:59238976-59238995 Msa0302460:three_prime_UTR 30.0%
! GCAGATAATGAAGGAATTTT+GGG + chr2_3:59238977-59238996 Msa0302460:three_prime_UTR 30.0%
! CAGATAATGAAGGAATTTTG+GGG + chr2_3:59238978-59238997 Msa0302460:three_prime_UTR 30.0%
!! GTTGATGGTTTCTTGAAGAT+TGG + chr2_3:59236769-59236788 Msa0302460:CDS 35.0%
TACCTATCATATTGCTCGAA+AGG + chr2_3:59236912-59236931 Msa0302460:CDS 35.0%
ACCTATCATATTGCTCGAAA+GGG + chr2_3:59236913-59236932 Msa0302460:CDS 35.0%
CAAAAGGGTTATAGCAAAAG+TGG - chr2_3:59236950-59236969 None:intergenic 35.0%
TTGGTATGTATGCATCTTTG+AGG + chr2_3:59237265-59237284 Msa0302460:intron 35.0%
TAATGTGATTTGAACTTGGG+AGG + chr2_3:59237291-59237310 Msa0302460:intron 35.0%
ATGAGAGAATCCGAATTTAG+GGG - chr2_3:59237349-59237368 None:intergenic 35.0%
ACTGACATATCAAGATCAGA+TGG - chr2_3:59237485-59237504 None:intergenic 35.0%
ACAAGGAACAAAGCATATCA+TGG - chr2_3:59237595-59237614 None:intergenic 35.0%
CTTTGTTCCTTGTTAATGAG+AGG + chr2_3:59237602-59237621 Msa0302460:intron 35.0%
AACAAGACCTCTCATTAACA+AGG - chr2_3:59237612-59237631 None:intergenic 35.0%
! CATTTAAAGAAGGTGCATAC+TGG + chr2_3:59237646-59237665 Msa0302460:CDS 35.0%
! TTTAAAGAAGGTGCATACTG+GGG + chr2_3:59237648-59237667 Msa0302460:CDS 35.0%
! ATTTTCCTTTCTACGCTTAC+AGG + chr2_3:59237738-59237757 Msa0302460:intron 35.0%
ATATGTTGGAACAGAATACG+GGG + chr2_3:59237776-59237795 Msa0302460:CDS 35.0%
ACTGGGTAATTCTCTCAATA+GGG + chr2_3:59237823-59237842 Msa0302460:intron 35.0%
!! TAGAGAGAGCACTTTATTTC+TGG + chr2_3:59237894-59237913 Msa0302460:intron 35.0%
TCTGTACATCAAAAGACCAA+GGG - chr2_3:59238104-59238123 None:intergenic 35.0%
TTCTGTACATCAAAAGACCA+AGG - chr2_3:59238105-59238124 None:intergenic 35.0%
GTACAGAAAAATGCCACATT+TGG + chr2_3:59238117-59238136 Msa0302460:intron 35.0%
TAAATGGCTGGAGTTTCTAA+TGG + chr2_3:59238247-59238266 Msa0302460:CDS 35.0%
TTCAGCTTTCTGTCAATTCT+TGG + chr2_3:59238390-59238409 Msa0302460:three_prime_UTR 35.0%
! TTCTGTCAATTCTTGGTCTT+TGG + chr2_3:59238397-59238416 Msa0302460:three_prime_UTR 35.0%
! GTTCTAGATCATGAGATGAA+GGG + chr2_3:59238531-59238550 Msa0302460:three_prime_UTR 35.0%
TTCTAGATCATGAGATGAAG+GGG + chr2_3:59238532-59238551 Msa0302460:three_prime_UTR 35.0%
CATATCAGTGTTAATGTCTG+AGG - chr2_3:59239027-59239046 None:intergenic 35.0%
CTCAGACATTAACACTGATA+TGG + chr2_3:59239025-59239044 Msa0302460:three_prime_UTR 35.0%
AGATGCTATGTCAGTATGTT+TGG - chr2_3:59239075-59239094 None:intergenic 35.0%
ATCCAAACTCGTTCCAAAAA+GGG + chr2_3:59239185-59239204 Msa0302460:three_prime_UTR 35.0%
! CACAAAAGTGTGACCACTTA+TGG - chr2_3:59236545-59236564 None:intergenic 40.0%
AGCTTGAATGTTAAGGAAGG+TGG - chr2_3:59236610-59236629 None:intergenic 40.0%
ACCTTCCTTAACATTCAAGC+TGG + chr2_3:59236609-59236628 Msa0302460:five_prime_UTR 40.0%
ACCAGCTTGAATGTTAAGGA+AGG - chr2_3:59236613-59236632 None:intergenic 40.0%
AGCTACCAGCTTGAATGTTA+AGG - chr2_3:59236617-59236636 None:intergenic 40.0%
GGTAGCTAAACAAAGCAAAG+AGG + chr2_3:59236630-59236649 Msa0302460:five_prime_UTR 40.0%
TCAAGAAACCATCAACAGTG+AGG - chr2_3:59236765-59236784 None:intergenic 40.0%
ACCCTTTCGAGCAATATGAT+AGG - chr2_3:59236917-59236936 None:intergenic 40.0%
CTTCTAAACATGTCTGTTCC+AGG + chr2_3:59237085-59237104 Msa0302460:intron 40.0%
! CATGATTGATTCATTTGGGG+TGG + chr2_3:59237173-59237192 Msa0302460:intron 40.0%
TTCACGTAATCACACACTTG+CGG - chr2_3:59237514-59237533 None:intergenic 40.0%
ATCTAAATCCTGAAAAGGGC+AGG + chr2_3:59237542-59237561 Msa0302460:intron 40.0%
GCTATAGCTGGTGTATATGT+TGG + chr2_3:59237762-59237781 Msa0302460:CDS 40.0%
GACTGGGTAATTCTCTCAAT+AGG + chr2_3:59237822-59237841 Msa0302460:intron 40.0%
TCAAAAGACCAAGGGAACAA+CGG - chr2_3:59238096-59238115 None:intergenic 40.0%
CAGAAAAATGCCACATTTGG+AGG + chr2_3:59238120-59238139 Msa0302460:intron 40.0%
ATCTGCAGCCAACAATGAAA+AGG + chr2_3:59238164-59238183 Msa0302460:CDS 40.0%
! CTTGTCTGCCTTTTCATTGT+TGG - chr2_3:59238175-59238194 None:intergenic 40.0%
AAGGCAGACAAGATTGTAGT+AGG + chr2_3:59238183-59238202 Msa0302460:CDS 40.0%
!! ATTGTAGTAGGTGCCATTAC+AGG + chr2_3:59238195-59238214 Msa0302460:CDS 40.0%
!! TTGTAGTAGGTGCCATTACA+GGG + chr2_3:59238196-59238215 Msa0302460:CDS 40.0%
ACTGCTGCAGCATTCATAAA+TGG + chr2_3:59238231-59238250 Msa0302460:CDS 40.0%
TTGAAAAGGGGTTGCACATT+TGG + chr2_3:59238303-59238322 Msa0302460:three_prime_UTR 40.0%
! GTCTCTTTTGTCTGTCTTAC+CGG + chr2_3:59238325-59238344 Msa0302460:three_prime_UTR 40.0%
!! GGTTCTAGATCATGAGATGA+AGG + chr2_3:59238530-59238549 Msa0302460:three_prime_UTR 40.0%
TCTAAGCAAGCATCCTCTTT+AGG - chr2_3:59238693-59238712 None:intergenic 40.0%
CACCTATTCCATTGGAATCA+AGG + chr2_3:59238733-59238752 Msa0302460:three_prime_UTR 40.0%
AACAGCCAAAGACAAAACTG+GGG - chr2_3:59238856-59238875 None:intergenic 40.0%
GAACAGCCAAAGACAAAACT+GGG - chr2_3:59238857-59238876 None:intergenic 40.0%
ATCTTCATCCACAGAAGTGA+CGG - chr2_3:59238880-59238899 None:intergenic 40.0%
TGTGGATGAAGATTTGAGTC+TGG + chr2_3:59238887-59238906 Msa0302460:three_prime_UTR 40.0%
GTGGATGAAGATTTGAGTCT+GGG + chr2_3:59238888-59238907 Msa0302460:three_prime_UTR 40.0%
AGTCTGGGCAAAAGAGTTAT+AGG + chr2_3:59238903-59238922 Msa0302460:three_prime_UTR 40.0%
AGAGTTATAGGTGGAAAGTC+TGG + chr2_3:59238915-59238934 Msa0302460:three_prime_UTR 40.0%
!!! TATCTGCACACTTTTAAGGC+TGG - chr2_3:59238964-59238983 None:intergenic 40.0%
AAAAGCCCTAAGACTCACAT+TGG + chr2_3:59239128-59239147 Msa0302460:three_prime_UTR 40.0%
CTTTGCCAATGTGAGTCTTA+GGG - chr2_3:59239136-59239155 None:intergenic 40.0%
CATCCAAACTCGTTCCAAAA+AGG + chr2_3:59239184-59239203 Msa0302460:three_prime_UTR 40.0%
ATGCCTCATAGCAGCATTTC+AGG + chr2_3:59236682-59236701 Msa0302460:CDS 45.0%
GATCCTGAAATGCTGCTATG+AGG - chr2_3:59236688-59236707 None:intergenic 45.0%
TCAATGACCACATCGACCTT+GGG - chr2_3:59236721-59236740 None:intergenic 45.0%
GTCGATGTGGTCATTGACAT+GGG + chr2_3:59236724-59236743 Msa0302460:CDS 45.0%
TCAACAGTGAGGTTGAGAAG+TGG - chr2_3:59236754-59236773 None:intergenic 45.0%
CTTCTCAACCTCACTGTTGA+TGG + chr2_3:59236754-59236773 Msa0302460:CDS 45.0%
!!! TTTTTCAGGTTGCAGCCACA+AGG + chr2_3:59236875-59236894 Msa0302460:intron 45.0%
!!! TTTTCAGGTTGCAGCCACAA+GGG + chr2_3:59236876-59236895 Msa0302460:intron 45.0%
TCACTACTTGAGACACTTCC+TGG - chr2_3:59237106-59237125 None:intergenic 45.0%
GACAGGAAAGCCCCTAAATT+CGG + chr2_3:59237336-59237355 Msa0302460:intron 45.0%
TAGCTCCTGTAAGCGTAGAA+AGG - chr2_3:59237746-59237765 None:intergenic 45.0%
TGAGAGAATTACCCAGTCTC+TGG - chr2_3:59237820-59237839 None:intergenic 45.0%
GATTCTTACCGTTGTTCCCT+TGG + chr2_3:59238085-59238104 Msa0302460:intron 45.0%
!! TGTAGTAGGTGCCATTACAG+GGG + chr2_3:59238197-59238216 Msa0302460:CDS 45.0%
CTGCAGCATTCATAAATGGC+TGG + chr2_3:59238235-59238254 Msa0302460:CDS 45.0%
! GTGCAACCCCTTTTCAAACA+TGG - chr2_3:59238300-59238319 None:intergenic 45.0%
AAAGAAACCTGGGCATGAAC+AGG + chr2_3:59238444-59238463 Msa0302460:three_prime_UTR 45.0%
TTGAAAACCTGTTCATGCCC+AGG - chr2_3:59238454-59238473 None:intergenic 45.0%
GCAAGATGCACCTATTCCAT+TGG + chr2_3:59238725-59238744 Msa0302460:three_prime_UTR 45.0%
GGCCTTGATTCCAATGGAAT+AGG - chr2_3:59238738-59238757 None:intergenic 45.0%
ACTATAGGTAGAGGCTGAGA+GGG - chr2_3:59238759-59238778 None:intergenic 45.0%
AACTATAGGTAGAGGCTGAG+AGG - chr2_3:59238760-59238779 None:intergenic 45.0%
GCAAATGTTGGACTCTGTCT+CGG + chr2_3:59238788-59238807 Msa0302460:three_prime_UTR 45.0%
CAAATGTTGGACTCTGTCTC+GGG + chr2_3:59238789-59238808 Msa0302460:three_prime_UTR 45.0%
AAATGTTGGACTCTGTCTCG+GGG + chr2_3:59238790-59238809 Msa0302460:three_prime_UTR 45.0%
! GTGATCCAAGTTTGGTGGTT+CGG + chr2_3:59238820-59238839 Msa0302460:three_prime_UTR 45.0%
!! TAGCACCCCAGTTTTGTCTT+TGG + chr2_3:59238848-59238867 Msa0302460:three_prime_UTR 45.0%
GGAACAGCCAAAGACAAAAC+TGG - chr2_3:59238858-59238877 None:intergenic 45.0%
TCTTCATCCACAGAAGTGAC+GGG - chr2_3:59238879-59238898 None:intergenic 45.0%
CTGGGCAAAAGAGTTATAGG+TGG + chr2_3:59238906-59238925 Msa0302460:three_prime_UTR 45.0%
ATAGCATCTAACACCAAGCC+TGG + chr2_3:59239086-59239105 Msa0302460:three_prime_UTR 45.0%
! TGCTTCTTAGTCTTCCTACC+AGG - chr2_3:59239107-59239126 None:intergenic 45.0%
CCTTTGCCAATGTGAGTCTT+AGG - chr2_3:59239137-59239156 None:intergenic 45.0%
CCTAAGACTCACATTGGCAA+AGG + chr2_3:59239134-59239153 Msa0302460:three_prime_UTR 45.0%
! CACATTGGCAAAGGTTCTGA+TGG + chr2_3:59239143-59239162 Msa0302460:three_prime_UTR 45.0%
!!! CACCCTTTTTGGAACGAGTT+TGG - chr2_3:59239190-59239209 None:intergenic 45.0%
!! AAAAATAAAAAATAAATAGT+TGG - chr2_3:59236856-59236875 None:intergenic 5.0%
!!! TTATTTTTTATTTTTTTTTC+AGG + chr2_3:59236861-59236880 Msa0302460:intron 5.0%
!!! AAATAATAATATATTGATTT+TGG - chr2_3:59237018-59237037 None:intergenic 5.0%
!! ATATATAAATTACTTATAAT+TGG - chr2_3:59237246-59237265 None:intergenic 5.0%
!! ATTATAAGTAATTTATATAT+TGG + chr2_3:59237246-59237265 Msa0302460:intron 5.0%
AGGATCAGTGTCTTCTCCCA+AGG + chr2_3:59236702-59236721 Msa0302460:CDS 50.0%
GTCAATGACCACATCGACCT+TGG - chr2_3:59236722-59236741 None:intergenic 50.0%
GGTCGATGTGGTCATTGACA+TGG + chr2_3:59236723-59236742 Msa0302460:CDS 50.0%
! ATGGGATTCCTGCCCTTTTC+AGG - chr2_3:59237553-59237572 None:intergenic 50.0%
ACGCTTACAGGAGCTATAGC+TGG + chr2_3:59237750-59237769 Msa0302460:intron 50.0%
TGTTGGAACAGAATACGGGG+TGG + chr2_3:59237779-59237798 Msa0302460:CDS 50.0%
TGTCACTGCACCTCCAAATG+TGG - chr2_3:59238133-59238152 None:intergenic 50.0%
! ACATTTGGAGGTGCAGTGAC+AGG + chr2_3:59238132-59238151 Msa0302460:CDS 50.0%
! CATTTGGAGGTGCAGTGACA+GGG + chr2_3:59238133-59238152 Msa0302460:CDS 50.0%
! ATTTGGAGGTGCAGTGACAG+GGG + chr2_3:59238134-59238153 Msa0302460:CDS 50.0%
GATGAAGGGGACGTGTTGTA+AGG + chr2_3:59238545-59238564 Msa0302460:three_prime_UTR 50.0%
AAGGGGACGTGTTGTAAGGT+AGG + chr2_3:59238549-59238568 Msa0302460:three_prime_UTR 50.0%
TGAGAGGGCCTTGATTCCAA+TGG - chr2_3:59238744-59238763 None:intergenic 50.0%
GAACACCGAACCACCAAACT+TGG - chr2_3:59238828-59238847 None:intergenic 50.0%
!!! TTTTAAGGCTGGCTGCTGAG+TGG - chr2_3:59238953-59238972 None:intergenic 50.0%
CATCTAACACCAAGCCTGGT+AGG + chr2_3:59239090-59239109 Msa0302460:three_prime_UTR 50.0%
! CTTAGTCTTCCTACCAGGCT+TGG - chr2_3:59239102-59239121 None:intergenic 50.0%
GTCTTCTCCCAAGGTCGATG+TGG + chr2_3:59236711-59236730 Msa0302460:CDS 55.0%
ATCCTCTGCAAGTGCCCTTG+TGG - chr2_3:59236893-59236912 None:intergenic 55.0%
GAACAGAATACGGGGTGGAG+AGG + chr2_3:59237784-59237803 Msa0302460:CDS 55.0%
! TTACCCAGTCTCTGGTGCCA+CGG - chr2_3:59237812-59237831 None:intergenic 55.0%
CGCAATTGCAGCCCCTGTAA+TGG - chr2_3:59238211-59238230 None:intergenic 55.0%
GAGGATGCTTGCTTAGAGCC+AGG + chr2_3:59238696-59238715 Msa0302460:three_prime_UTR 55.0%
! GGTGCATCTTGCTCTAGACC+TGG - chr2_3:59238717-59238736 None:intergenic 55.0%
! GAGGGGGTGTGATCCAAGTT+TGG + chr2_3:59238812-59238831 Msa0302460:three_prime_UTR 55.0%
GGGGTGTGATCCAAGTTTGG+TGG + chr2_3:59238815-59238834 Msa0302460:three_prime_UTR 55.0%
!! AGCCACAAGGGCACTTGCAG+AGG + chr2_3:59236888-59236907 Msa0302460:CDS 60.0%
GATCCGTGGCACCAGAGACT+GGG + chr2_3:59237806-59237825 Msa0302460:CDS 60.0%
TGTTGGACTCTGTCTCGGGG+AGG + chr2_3:59238793-59238812 Msa0302460:three_prime_UTR 60.0%
GTTGGACTCTGTCTCGGGGA+GGG + chr2_3:59238794-59238813 Msa0302460:three_prime_UTR 60.0%
TTGGACTCTGTCTCGGGGAG+GGG + chr2_3:59238795-59238814 Msa0302460:three_prime_UTR 60.0%
GGCTGTTCCCGTCACTTCTG+TGG + chr2_3:59238869-59238888 Msa0302460:three_prime_UTR 60.0%
GCTGGCTGCTGAGTGGTTAC+TGG - chr2_3:59238946-59238965 None:intergenic 60.0%
TACGGGGTGGAGAGGATCCG+TGG + chr2_3:59237792-59237811 Msa0302460:CDS 65.0%
GGATCCGTGGCACCAGAGAC+TGG + chr2_3:59237805-59237824 Msa0302460:CDS 65.0%
AGGTGCAGTGACAGGGGCTC+TGG + chr2_3:59238140-59238159 Msa0302460:CDS 65.0%
TGGACTCTGTCTCGGGGAGG+GGG + chr2_3:59238796-59238815 Msa0302460:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 59236531 59239214 59236531 ID=Msa0302460;Name=Msa0302460
chr2_3 mRNA 59236531 59239214 59236531 ID=Msa0302460-mRNA-1;Parent=Msa0302460;Name=Msa0302460-mRNA-1;_AED=0.29;_eAED=0.29;_QI=151|1|1|1|1|1|6|948|147
chr2_3 exon 59236531 59236795 59236531 ID=Msa0302460-mRNA-1:exon:19152;Parent=Msa0302460-mRNA-1
chr2_3 exon 59236883 59236934 59236883 ID=Msa0302460-mRNA-1:exon:19153;Parent=Msa0302460-mRNA-1
chr2_3 exon 59237107 59237138 59237107 ID=Msa0302460-mRNA-1:exon:19154;Parent=Msa0302460-mRNA-1
chr2_3 exon 59237636 59237669 59237636 ID=Msa0302460-mRNA-1:exon:19155;Parent=Msa0302460-mRNA-1
chr2_3 exon 59237760 59237827 59237760 ID=Msa0302460-mRNA-1:exon:19156;Parent=Msa0302460-mRNA-1
chr2_3 exon 59238123 59239214 59238123 ID=Msa0302460-mRNA-1:exon:19157;Parent=Msa0302460-mRNA-1
chr2_3 five_prime_UTR 59236531 59236681 59236531 ID=Msa0302460-mRNA-1:five_prime_utr;Parent=Msa0302460-mRNA-1
chr2_3 CDS 59236682 59236795 59236682 ID=Msa0302460-mRNA-1:cds;Parent=Msa0302460-mRNA-1
chr2_3 CDS 59236883 59236934 59236883 ID=Msa0302460-mRNA-1:cds;Parent=Msa0302460-mRNA-1
chr2_3 CDS 59237107 59237138 59237107 ID=Msa0302460-mRNA-1:cds;Parent=Msa0302460-mRNA-1
chr2_3 CDS 59237636 59237669 59237636 ID=Msa0302460-mRNA-1:cds;Parent=Msa0302460-mRNA-1
chr2_3 CDS 59237760 59237827 59237760 ID=Msa0302460-mRNA-1:cds;Parent=Msa0302460-mRNA-1
chr2_3 CDS 59238123 59238266 59238123 ID=Msa0302460-mRNA-1:cds;Parent=Msa0302460-mRNA-1
chr2_3 three_prime_UTR 59238267 59239214 59238267 ID=Msa0302460-mRNA-1:three_prime_utr;Parent=Msa0302460-mRNA-1
Gene Sequence

>Msa0302460

ATGCCTCATAGCAGCATTTCAGGATCAGTGTCTTCTCCCAAGGTCGATGTGGTCATTGACATGGGCAATCCACTTCTCAACCTCACTGTTGATGGTTTCTTGAAGATTGGAGCTGTTGCAGCCACAAGGGCACTTGCAGAGGATACCTATCATATTGCTCGAAAGGGAAGTGTCTCAAGTAGTGATTTTGAGAAAACTCTGAAGAAGACATTTAAAGAAGGTGCATACTGGGGAGCTATAGCTGGTGTATATGTTGGAACAGAATACGGGGTGGAGAGGATCCGTGGCACCAGAGACTGGAAAAATGCCACATTTGGAGGTGCAGTGACAGGGGCTCTGGTATCTGCAGCCAACAATGAAAAGGCAGACAAGATTGTAGTAGGTGCCATTACAGGGGCTGCAATTGCGACTGCTGCAGCATTCATAAATGGCTGGAGTTTCTAA
Protein sequence

>Msa0302460

MPHSSISGSVSSPKVDVVIDMGNPLLNLTVDGFLKIGAVAATRALAEDTYHIARKGSVSSSDFEKTLKKTFKEGAYWGAIAGVYVGTEYGVERIRGTRDWKNATFGGAVTGALVSAANNEKADKIVVGAITGAAIATAAAFINGWSF*