AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0307500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0307500 MtrunA17_Chr3g0115561 93.210 162 11 0 1 162 1 162 5.34e-113 317
Msa0307500 MtrunA17_Chr3g0124301 41.406 128 73 2 28 153 25 152 2.73e-31 110
Msa0307500 MtrunA17_Chr2g0283801 35.256 156 96 3 3 155 4 157 8.21e-25 94.0
Msa0307500 MtrunA17_Chr2g0316111 33.594 128 82 2 28 155 43 167 5.94e-19 79.0
Msa0307500 MtrunA17_Chr2g0316151 33.594 128 82 2 28 155 43 167 7.27e-19 79.0
Msa0307500 MtrunA17_Chr2g0316121 28.947 152 96 4 18 160 20 168 1.77e-15 69.7
Msa0307500 MtrunA17_Chr2g0316161 28.966 145 93 3 18 155 20 161 1.77e-15 69.7
Msa0307500 MtrunA17_Chr2g0316051 28.906 128 88 3 28 155 36 160 4.27e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0307500 AT4G08685.1 48.734 158 81 0 3 160 2 159 1.35e-54 170
Msa0307500 AT1G78040.1 38.182 165 97 3 3 162 2 166 1.77e-33 116
Msa0307500 AT1G78040.3 38.182 165 97 3 3 162 2 166 1.77e-33 116
Msa0307500 AT1G78040.2 38.182 165 97 3 3 162 2 166 1.77e-33 116
Msa0307500 AT5G10130.1 40.909 154 86 2 3 154 4 154 4.22e-30 107
Msa0307500 AT4G18596.1 32.317 164 102 3 1 155 1 164 1.32e-22 89.0
Msa0307500 AT4G18596.2 32.317 164 102 3 1 155 47 210 2.99e-22 89.0
Msa0307500 AT5G45880.1 31.325 166 103 3 1 155 1 166 2.48e-21 85.5
Msa0307500 AT1G29140.1 35.075 134 83 2 26 155 30 163 1.64e-20 83.2

Find 89 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTATATATATGTTAT+TGG 0.205323 2_4:-407050 Msa0307500:three_prime_UTR
CTGTCTTGTGATGTTATCTT+TGG 0.219851 2_4:-408711 Msa0307500:five_prime_UTR
ATCTTTGGTAATGTACATTT+TGG 0.224758 2_4:-408696 Msa0307500:five_prime_UTR
TAGTTTATTTACTTTGGATT+TGG 0.238080 2_4:-407136 Msa0307500:three_prime_UTR
AGTTTATTTACTTTGGATTT+GGG 0.259659 2_4:-407135 Msa0307500:three_prime_UTR
AGAAATCTTAAGTGTCTTAA+TGG 0.270502 2_4:+408660 None:intergenic
CTTCAAGCAGAAAGGGTTCT+TGG 0.299969 2_4:+408334 None:intergenic
GCATAGAGCCATTCTTGAAT+TGG 0.350352 2_4:-406862 Msa0307500:three_prime_UTR
TCAATCTTCATCCTTCACTA+TGG 0.355771 2_4:-408423 Msa0307500:exon
GTGGAAACAAACTGTTCATT+TGG 0.356394 2_4:+408867 None:intergenic
AGCCATTCTTGAATTGGCTT+TGG 0.357937 2_4:-406856 Msa0307500:three_prime_UTR
CAAGAACCCTTTCTGCTTGA+AGG 0.359097 2_4:-408333 Msa0307500:CDS
CAATGAATTCTTTCATATGA+TGG 0.367752 2_4:-406929 Msa0307500:three_prime_UTR
AATTCCTAGTTTATTTACTT+TGG 0.368713 2_4:-407142 Msa0307500:three_prime_UTR
ATTGGCAAACCTGTCATTAG+AGG 0.373550 2_4:+407251 None:intergenic
CCAGTTGCATCAGTCTTCCC+TGG 0.379925 2_4:+407417 None:intergenic
CTTAATTATATTGTTTATTG+AGG 0.395561 2_4:-407021 Msa0307500:three_prime_UTR
AAGCAGAAAGGGTTCTTGGC+TGG 0.404947 2_4:+408338 None:intergenic
CCTCAAGCAAATGCAAGAAT+TGG 0.407401 2_4:-407181 Msa0307500:CDS
ATTTGATTAAATCTTGATGA+TGG 0.416149 2_4:-407099 Msa0307500:three_prime_UTR
ATGTCAGAGGAGGTTGCTTC+TGG 0.416314 2_4:-407216 Msa0307500:CDS
AGAGGCAATGCCGTTGTAAC+GGG 0.421384 2_4:+407269 None:intergenic
AGTTGCAGCGAAAGAGCACC+TGG 0.422043 2_4:-407318 Msa0307500:CDS
TGGAGTAAAGTTGGAGGAAT+GGG 0.424058 2_4:+408536 None:intergenic
CTTTCGCTGCAACTACGCTC+AGG 0.426036 2_4:+407327 None:intergenic
AGAAGAAGAAGAATGAGAAA+TGG 0.426492 2_4:+408461 None:intergenic
TGTTCTTCCAGCGATGGTTA+TGG 0.427430 2_4:-408369 Msa0307500:CDS
TCTGCTATCACTCACATTGC+TGG 0.439139 2_4:-408269 Msa0307500:intron
GTATGTGACCCTTGCCGTGC+TGG 0.440078 2_4:-408302 Msa0307500:CDS
GTGTGTCAGTGGAGTAAAGT+TGG 0.445445 2_4:+408527 None:intergenic
AGTGATGAAGGCAAAATTAT+CGG 0.452872 2_4:-408932 Msa0307500:five_prime_UTR
CTTAGGTGCCGAGGTTATGC+TGG 0.455452 2_4:-407481 Msa0307500:intron
AGCAGAAAGGGTTCTTGGCT+GGG 0.458995 2_4:+408339 None:intergenic
CCAGGGAAGACTGATGCAAC+TGG 0.462248 2_4:-407417 Msa0307500:CDS
TAGTGTAATTATGTCATGTT+TGG 0.468469 2_4:-406805 Msa0307500:three_prime_UTR
ATATACAATCTATGTGGATC+AGG 0.471477 2_4:-407391 Msa0307500:CDS
CACGACCCTTCAAGCAGAAA+GGG 0.481464 2_4:+408327 None:intergenic
CCAATTCTTGCATTTGCTTG+AGG 0.488555 2_4:+407181 None:intergenic
AGGGTTCTTGGCTGGGCGAA+TGG 0.488766 2_4:+408346 None:intergenic
GAAGTTGTGTTCTCAAAGCC+AGG 0.495130 2_4:-407435 Msa0307500:CDS
CAGAACAATAGAATATCAAA+GGG 0.503003 2_4:+408731 None:intergenic
AAGAACCCTTTCTGCTTGAA+GGG 0.515228 2_4:-408332 Msa0307500:CDS
GTGGAGTAAAGTTGGAGGAA+TGG 0.516719 2_4:+408535 None:intergenic
TGATGTGGTGTAGAGAAGGA+TGG 0.520939 2_4:+408758 None:intergenic
ACAGAACAATAGAATATCAA+AGG 0.521477 2_4:+408730 None:intergenic
TTATTGAGGATCATGTGTCT+TGG 0.527504 2_4:-407007 Msa0307500:three_prime_UTR
TAGAGGCAATGCCGTTGTAA+CGG 0.528529 2_4:+407268 None:intergenic
ATGAACAGTTTGTTTCCACT+TGG 0.532879 2_4:-408863 Msa0307500:five_prime_UTR
ATCCAAAGCCAATTCAAGAA+TGG 0.535606 2_4:+406854 None:intergenic
TAGTCTCAGTTCTGTTGCTG+TGG 0.539457 2_4:-406974 Msa0307500:three_prime_UTR
AAAGTGTAAGAAAGTGATGA+AGG 0.543243 2_4:-408944 None:intergenic
ACACGACCCTTCAAGCAGAA+AGG 0.545423 2_4:+408326 None:intergenic
CGAATGGCCATAACCATCGC+TGG 0.546740 2_4:+408362 None:intergenic
TAGAGAAGGATGGATAGTAG+AGG 0.559668 2_4:+408768 None:intergenic
TTTCGCTGCAACTACGCTCA+GGG 0.560515 2_4:+407328 None:intergenic
ACGGCATTGCCTCTAATGAC+AGG 0.562012 2_4:-407260 Msa0307500:CDS
CAATTAAAAGGGTGTGTCAG+TGG 0.563724 2_4:+408516 None:intergenic
AAATCCAAAGTAAATAAACT+AGG 0.569739 2_4:+407138 None:intergenic
CTTGCATTCCAGCATAACCT+CGG 0.573523 2_4:+407473 None:intergenic
GTTATTCTCACCCGTTACAA+CGG 0.576439 2_4:-407279 Msa0307500:CDS
TGTCAGTGGAGTAAAGTTGG+AGG 0.582649 2_4:+408530 None:intergenic
ACACGTGCTTGGTCACGCCC+AGG 0.584341 2_4:+407300 None:intergenic
TGACATGTATAACATTGCAT+TGG 0.586829 2_4:+407233 None:intergenic
GTTTCATAACCAGCACGGCA+AGG 0.587642 2_4:+408293 None:intergenic
AACTCTTGAAGCCATAGTGA+AGG 0.589099 2_4:+408412 None:intergenic
GGTTGCTTCTGGCTGTGCTG+AGG 0.590498 2_4:-407205 Msa0307500:CDS
GGAATGATGTGGTGTAGAGA+AGG 0.595014 2_4:+408754 None:intergenic
TTTGTGTGTTCTTCCAGCGA+TGG 0.596076 2_4:-408375 Msa0307500:CDS
TGGAGAATATACAATCTATG+TGG 0.605358 2_4:-407397 Msa0307500:CDS
ATGAATCTGCTTAGGTGCCG+AGG 0.607207 2_4:-407490 Msa0307500:intron
ATAAACAATATAATTAAGCA+TGG 0.612028 2_4:+407026 None:intergenic
TTTCATAACCAGCACGGCAA+GGG 0.614551 2_4:+408294 None:intergenic
ACATAATTACACTAACATGT+AGG 0.616143 2_4:+406814 None:intergenic
GGGTGAGAATAACACGTGCT+TGG 0.627588 2_4:+407289 None:intergenic
TGCAATGTTATACATGTCAG+AGG 0.631370 2_4:-407229 Msa0307500:CDS
AAGTTGTGTTCTCAAAGCCA+GGG 0.636901 2_4:-407434 Msa0307500:CDS
AAAATTATCGGCGCGTCACG+CGG 0.638390 2_4:-408920 Msa0307500:five_prime_UTR
GTGCTACATGTGACTCCAAG+TGG 0.649681 2_4:+408848 None:intergenic
TTATGCTGGAATGCAAGCAA+AGG 0.653268 2_4:-407467 Msa0307500:CDS
TTGTGTATGCTCATATGTGG+TGG 0.660006 2_4:+408581 None:intergenic
CAGAAGTTTCATAACCAGCA+CGG 0.663268 2_4:+408288 None:intergenic
GGATCAGGATCGTGCAGATG+AGG 0.670189 2_4:-407376 Msa0307500:CDS
GCGTTGTGTATGCTCATATG+TGG 0.676793 2_4:+408578 None:intergenic
GAACAATAGAATATCAAAGG+GGG 0.678493 2_4:+408733 None:intergenic
GATTTCTCATGATAATGACG+TGG 0.681126 2_4:-408644 Msa0307500:five_prime_UTR
AATGTTATACATGTCAGAGG+AGG 0.692196 2_4:-407226 Msa0307500:CDS
AGAACAATAGAATATCAAAG+GGG 0.697815 2_4:+408732 None:intergenic
GAGTGCATTGTAAATTAACG+TGG 0.705392 2_4:+408603 None:intergenic
GTTGCAGCGAAAGAGCACCT+GGG 0.708218 2_4:-407317 Msa0307500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATCTTTGTTAATTAA+TGG - chr2_4:407753-407772 Msa0307500:intron 10.0%
!!! TTTAATTAGAAGTATAAAAA+AGG - chr2_4:408100-408119 Msa0307500:intron 10.0%
!!! TTAATTAGAAGTATAAAAAA+GGG - chr2_4:408101-408120 Msa0307500:intron 10.0%
!! AAAATATCTTTGTTAATTAA+TGG - chr2_4:407753-407772 Msa0307500:intron 10.0%
!!! TTTAATTAGAAGTATAAAAA+AGG - chr2_4:408100-408119 Msa0307500:intron 10.0%
!!! TTAATTAGAAGTATAAAAAA+GGG - chr2_4:408101-408120 Msa0307500:intron 10.0%
!!! TTGAAGATTTTCAATTAAAA+GGG + chr2_4:407188-407207 None:intergenic 15.0%
!!! ATTGAAGATTTTCAATTAAA+AGG + chr2_4:407189-407208 None:intergenic 15.0%
!!! ACTTTAAATTCCTTAATTTT+TGG - chr2_4:407541-407560 Msa0307500:intron 15.0%
!! TATTATATGATAGAACATTA+TGG - chr2_4:407993-408012 Msa0307500:intron 15.0%
!! ATTATATGATAGAACATTAT+GGG - chr2_4:407994-408013 Msa0307500:intron 15.0%
!! TATGCTTATATATATGTTAT+TGG - chr2_4:408640-408659 Msa0307500:five_prime_UTR 15.0%
!! ATAAACAATATAATTAAGCA+TGG + chr2_4:408667-408686 None:intergenic 15.0%
!! CTTAATTATATTGTTTATTG+AGG - chr2_4:408669-408688 Msa0307500:five_prime_UTR 15.0%
!!! TTGAAGATTTTCAATTAAAA+GGG + chr2_4:407188-407207 None:intergenic 15.0%
!!! ATTGAAGATTTTCAATTAAA+AGG + chr2_4:407189-407208 None:intergenic 15.0%
!!! ACTTTAAATTCCTTAATTTT+TGG - chr2_4:407541-407560 Msa0307500:intron 15.0%
!! TATTATATGATAGAACATTA+TGG - chr2_4:407993-408012 Msa0307500:intron 15.0%
!! ATTATATGATAGAACATTAT+GGG - chr2_4:407994-408013 Msa0307500:intron 15.0%
!! TATGCTTATATATATGTTAT+TGG - chr2_4:408640-408659 Msa0307500:five_prime_UTR 15.0%
!! ATAAACAATATAATTAAGCA+TGG + chr2_4:408667-408686 None:intergenic 15.0%
!! CTTAATTATATTGTTTATTG+AGG - chr2_4:408669-408688 Msa0307500:five_prime_UTR 15.0%
!!! ATAATTATAGTTTTTGTAGC+TGG + chr2_4:406876-406895 None:intergenic 20.0%
!! TTGAATGAAAAACTTAAACT+TGG + chr2_4:407484-407503 None:intergenic 20.0%
!! ATAGTGAAATTAAACCAAAA+GGG + chr2_4:407516-407535 None:intergenic 20.0%
!! AATAGTGAAATTAAACCAAA+AGG + chr2_4:407517-407536 None:intergenic 20.0%
!!! AATTCCTTAATTTTTGGAAA+TGG - chr2_4:407547-407566 Msa0307500:intron 20.0%
!!! TCAATTTTGCTTTAGATTTT+AGG - chr2_4:407583-407602 Msa0307500:intron 20.0%
!!! CAATTTTGCTTTAGATTTTA+GGG - chr2_4:407584-407603 Msa0307500:intron 20.0%
!! TTCACAAATTAGTGTAATAT+AGG - chr2_4:407681-407700 Msa0307500:intron 20.0%
!! TCACAAATTAGTGTAATATA+GGG - chr2_4:407682-407701 Msa0307500:intron 20.0%
!! ATCTATTGTTTCAAATAATC+TGG + chr2_4:407835-407854 None:intergenic 20.0%
!! CTTATAATTTATAATCCTCA+TGG - chr2_4:408128-408147 Msa0307500:intron 20.0%
!! AATTCCTAGTTTATTTACTT+TGG - chr2_4:408548-408567 Msa0307500:five_prime_UTR 20.0%
!! AAATCCAAAGTAAATAAACT+AGG + chr2_4:408555-408574 None:intergenic 20.0%
!! TAGTTTATTTACTTTGGATT+TGG - chr2_4:408554-408573 Msa0307500:five_prime_UTR 20.0%
!! AGTTTATTTACTTTGGATTT+GGG - chr2_4:408555-408574 Msa0307500:five_prime_UTR 20.0%
!! ATTTGATTAAATCTTGATGA+TGG - chr2_4:408591-408610 Msa0307500:five_prime_UTR 20.0%
!!! ATAATTATAGTTTTTGTAGC+TGG + chr2_4:406876-406895 None:intergenic 20.0%
!! TTGAATGAAAAACTTAAACT+TGG + chr2_4:407484-407503 None:intergenic 20.0%
!! ATAGTGAAATTAAACCAAAA+GGG + chr2_4:407516-407535 None:intergenic 20.0%
!! AATAGTGAAATTAAACCAAA+AGG + chr2_4:407517-407536 None:intergenic 20.0%
!!! AATTCCTTAATTTTTGGAAA+TGG - chr2_4:407547-407566 Msa0307500:intron 20.0%
!!! TCAATTTTGCTTTAGATTTT+AGG - chr2_4:407583-407602 Msa0307500:intron 20.0%
!!! CAATTTTGCTTTAGATTTTA+GGG - chr2_4:407584-407603 Msa0307500:intron 20.0%
!! TTCACAAATTAGTGTAATAT+AGG - chr2_4:407681-407700 Msa0307500:intron 20.0%
!! TCACAAATTAGTGTAATATA+GGG - chr2_4:407682-407701 Msa0307500:intron 20.0%
!! ATCTATTGTTTCAAATAATC+TGG + chr2_4:407835-407854 None:intergenic 20.0%
!! CTTATAATTTATAATCCTCA+TGG - chr2_4:408128-408147 Msa0307500:intron 20.0%
!! AATTCCTAGTTTATTTACTT+TGG - chr2_4:408548-408567 Msa0307500:five_prime_UTR 20.0%
!! AAATCCAAAGTAAATAAACT+AGG + chr2_4:408555-408574 None:intergenic 20.0%
!! TAGTTTATTTACTTTGGATT+TGG - chr2_4:408554-408573 Msa0307500:five_prime_UTR 20.0%
!! AGTTTATTTACTTTGGATTT+GGG - chr2_4:408555-408574 Msa0307500:five_prime_UTR 20.0%
!! ATTTGATTAAATCTTGATGA+TGG - chr2_4:408591-408610 Msa0307500:five_prime_UTR 20.0%
! ATAGTAGAGGATAAAAAGAA+TGG + chr2_4:406912-406931 None:intergenic 25.0%
! AGAACAATAGAATATCAAAG+GGG + chr2_4:406961-406980 None:intergenic 25.0%
! CAGAACAATAGAATATCAAA+GGG + chr2_4:406962-406981 None:intergenic 25.0%
! ACAGAACAATAGAATATCAA+AGG + chr2_4:406963-406982 None:intergenic 25.0%
!!! ATCTTTGGTAATGTACATTT+TGG - chr2_4:406994-407013 Msa0307500:three_prime_UTR 25.0%
!!! TCTTTGGTAATGTACATTTT+GGG - chr2_4:406995-407014 Msa0307500:three_prime_UTR 25.0%
! AGAAATCTTAAGTGTCTTAA+TGG + chr2_4:407033-407052 None:intergenic 25.0%
! AACACCATTTCCAAAAATTA+AGG + chr2_4:407554-407573 None:intergenic 25.0%
! TTTGATTAATGAATCTGCTT+AGG - chr2_4:408192-408211 Msa0307500:intron 25.0%
! CAATGAATTCTTTCATATGA+TGG - chr2_4:408761-408780 Msa0307500:five_prime_UTR 25.0%
! ACATAATTACACTAACATGT+AGG + chr2_4:408879-408898 None:intergenic 25.0%
! TAGTGTAATTATGTCATGTT+TGG - chr2_4:408885-408904 Msa0307500:five_prime_UTR 25.0%
! CTCATAAAAAATCAATCGAA+TGG + chr2_4:408915-408934 None:intergenic 25.0%
! ATAGTAGAGGATAAAAAGAA+TGG + chr2_4:406912-406931 None:intergenic 25.0%
! AGAACAATAGAATATCAAAG+GGG + chr2_4:406961-406980 None:intergenic 25.0%
! CAGAACAATAGAATATCAAA+GGG + chr2_4:406962-406981 None:intergenic 25.0%
! ACAGAACAATAGAATATCAA+AGG + chr2_4:406963-406982 None:intergenic 25.0%
!!! ATCTTTGGTAATGTACATTT+TGG - chr2_4:406994-407013 Msa0307500:three_prime_UTR 25.0%
!!! TCTTTGGTAATGTACATTTT+GGG - chr2_4:406995-407014 Msa0307500:three_prime_UTR 25.0%
! AGAAATCTTAAGTGTCTTAA+TGG + chr2_4:407033-407052 None:intergenic 25.0%
! AACACCATTTCCAAAAATTA+AGG + chr2_4:407554-407573 None:intergenic 25.0%
! TTTGATTAATGAATCTGCTT+AGG - chr2_4:408192-408211 Msa0307500:intron 25.0%
! CAATGAATTCTTTCATATGA+TGG - chr2_4:408761-408780 Msa0307500:five_prime_UTR 25.0%
! ACATAATTACACTAACATGT+AGG + chr2_4:408879-408898 None:intergenic 25.0%
! TAGTGTAATTATGTCATGTT+TGG - chr2_4:408885-408904 Msa0307500:five_prime_UTR 25.0%
! CTCATAAAAAATCAATCGAA+TGG + chr2_4:408915-408934 None:intergenic 25.0%
GAACAATAGAATATCAAAGG+GGG + chr2_4:406960-406979 None:intergenic 30.0%
AGAAGAAGAAGAATGAGAAA+TGG + chr2_4:407232-407251 None:intergenic 30.0%
AAGAGATCTGAGAGTAAAAA+GGG + chr2_4:407451-407470 None:intergenic 30.0%
AAAGAGATCTGAGAGTAAAA+AGG + chr2_4:407452-407471 None:intergenic 30.0%
!!! TTCAAATTTGTTGACCCTTT+TGG - chr2_4:407499-407518 Msa0307500:intron 30.0%
!!! TGCTTTAGATTTTAGGGTTT+AGG - chr2_4:407590-407609 Msa0307500:intron 30.0%
!!! GCTTTAGATTTTAGGGTTTA+GGG - chr2_4:407591-407610 Msa0307500:intron 30.0%
!!! AACCACTCTTTTTGAGATAA+TGG - chr2_4:407638-407657 Msa0307500:intron 30.0%
TTGTTAAATGAATGACCTTG+CGG - chr2_4:407794-407813 Msa0307500:intron 30.0%
TGTTTGATTCATGTAACCAT+TGG - chr2_4:408019-408038 Msa0307500:intron 30.0%
TGTGTTAACAAACTACCATA+TGG + chr2_4:408164-408183 None:intergenic 30.0%
TGGAGAATATACAATCTATG+TGG - chr2_4:408293-408312 Msa0307500:CDS 30.0%
ATATACAATCTATGTGGATC+AGG - chr2_4:408299-408318 Msa0307500:CDS 30.0%
TGACATGTATAACATTGCAT+TGG + chr2_4:408460-408479 None:intergenic 30.0%
GAACAATAGAATATCAAAGG+GGG + chr2_4:406960-406979 None:intergenic 30.0%
AGAAGAAGAAGAATGAGAAA+TGG + chr2_4:407232-407251 None:intergenic 30.0%
AAGAGATCTGAGAGTAAAAA+GGG + chr2_4:407451-407470 None:intergenic 30.0%
AAAGAGATCTGAGAGTAAAA+AGG + chr2_4:407452-407471 None:intergenic 30.0%
!!! TTCAAATTTGTTGACCCTTT+TGG - chr2_4:407499-407518 Msa0307500:intron 30.0%
!!! TGCTTTAGATTTTAGGGTTT+AGG - chr2_4:407590-407609 Msa0307500:intron 30.0%
!!! GCTTTAGATTTTAGGGTTTA+GGG - chr2_4:407591-407610 Msa0307500:intron 30.0%
!!! AACCACTCTTTTTGAGATAA+TGG - chr2_4:407638-407657 Msa0307500:intron 30.0%
TTGTTAAATGAATGACCTTG+CGG - chr2_4:407794-407813 Msa0307500:intron 30.0%
TGTTTGATTCATGTAACCAT+TGG - chr2_4:408019-408038 Msa0307500:intron 30.0%
TGTGTTAACAAACTACCATA+TGG + chr2_4:408164-408183 None:intergenic 30.0%
TGGAGAATATACAATCTATG+TGG - chr2_4:408293-408312 Msa0307500:CDS 30.0%
ATATACAATCTATGTGGATC+AGG - chr2_4:408299-408318 Msa0307500:CDS 30.0%
TGACATGTATAACATTGCAT+TGG + chr2_4:408460-408479 None:intergenic 30.0%
GTGGAAACAAACTGTTCATT+TGG + chr2_4:406826-406845 None:intergenic 35.0%
! ATGAACAGTTTGTTTCCACT+TGG - chr2_4:406827-406846 Msa0307500:three_prime_UTR 35.0%
! TAAAAAGAATGGCGGTGTTT+TGG + chr2_4:406901-406920 None:intergenic 35.0%
GTAGAGGATAAAAAGAATGG+CGG + chr2_4:406909-406928 None:intergenic 35.0%
CTGTCTTGTGATGTTATCTT+TGG - chr2_4:406979-406998 Msa0307500:three_prime_UTR 35.0%
! GATTTCTCATGATAATGACG+TGG - chr2_4:407046-407065 Msa0307500:three_prime_UTR 35.0%
GAGTGCATTGTAAATTAACG+TGG + chr2_4:407090-407109 None:intergenic 35.0%
TCAATCTTCATCCTTCACTA+TGG - chr2_4:407267-407286 Msa0307500:CDS 35.0%
AGAGATCTGAGAGTAAAAAG+GGG + chr2_4:407450-407469 None:intergenic 35.0%
!!! TTAGATTTTAGGGTTTAGGG+AGG - chr2_4:407594-407613 Msa0307500:intron 35.0%
!!! TAGATTTTAGGGTTTAGGGA+GGG - chr2_4:407595-407614 Msa0307500:intron 35.0%
CTCCATTATCTCAAAAAGAG+TGG + chr2_4:407643-407662 None:intergenic 35.0%
TTGCTTCTAATGCAAAGATC+TGG - chr2_4:407946-407965 Msa0307500:intron 35.0%
TGCTTCTAATGCAAAGATCT+GGG - chr2_4:407947-407966 Msa0307500:intron 35.0%
ACAACAGTAGTAACAACCAA+TGG + chr2_4:408038-408057 None:intergenic 35.0%
TATGGAAAATGTTCTCCATG+AGG + chr2_4:408146-408165 None:intergenic 35.0%
! CATGGAGAACATTTTCCATA+TGG - chr2_4:408146-408165 Msa0307500:intron 35.0%
TGCAATGTTATACATGTCAG+AGG - chr2_4:408461-408480 Msa0307500:five_prime_UTR 35.0%
AATGTTATACATGTCAGAGG+AGG - chr2_4:408464-408483 Msa0307500:five_prime_UTR 35.0%
TTATTGAGGATCATGTGTCT+TGG - chr2_4:408683-408702 Msa0307500:five_prime_UTR 35.0%
! ATATGATGGTTTTCGTTCTG+TGG - chr2_4:408775-408794 Msa0307500:five_prime_UTR 35.0%
ATCCAAAGCCAATTCAAGAA+TGG + chr2_4:408839-408858 None:intergenic 35.0%
GTGGAAACAAACTGTTCATT+TGG + chr2_4:406826-406845 None:intergenic 35.0%
! ATGAACAGTTTGTTTCCACT+TGG - chr2_4:406827-406846 Msa0307500:three_prime_UTR 35.0%
! TAAAAAGAATGGCGGTGTTT+TGG + chr2_4:406901-406920 None:intergenic 35.0%
GTAGAGGATAAAAAGAATGG+CGG + chr2_4:406909-406928 None:intergenic 35.0%
CTGTCTTGTGATGTTATCTT+TGG - chr2_4:406979-406998 Msa0307500:three_prime_UTR 35.0%
! GATTTCTCATGATAATGACG+TGG - chr2_4:407046-407065 Msa0307500:three_prime_UTR 35.0%
GAGTGCATTGTAAATTAACG+TGG + chr2_4:407090-407109 None:intergenic 35.0%
TCAATCTTCATCCTTCACTA+TGG - chr2_4:407267-407286 Msa0307500:CDS 35.0%
AGAGATCTGAGAGTAAAAAG+GGG + chr2_4:407450-407469 None:intergenic 35.0%
!!! TTAGATTTTAGGGTTTAGGG+AGG - chr2_4:407594-407613 Msa0307500:intron 35.0%
!!! TAGATTTTAGGGTTTAGGGA+GGG - chr2_4:407595-407614 Msa0307500:intron 35.0%
CTCCATTATCTCAAAAAGAG+TGG + chr2_4:407643-407662 None:intergenic 35.0%
TTGCTTCTAATGCAAAGATC+TGG - chr2_4:407946-407965 Msa0307500:intron 35.0%
TGCTTCTAATGCAAAGATCT+GGG - chr2_4:407947-407966 Msa0307500:intron 35.0%
ACAACAGTAGTAACAACCAA+TGG + chr2_4:408038-408057 None:intergenic 35.0%
TATGGAAAATGTTCTCCATG+AGG + chr2_4:408146-408165 None:intergenic 35.0%
! CATGGAGAACATTTTCCATA+TGG - chr2_4:408146-408165 Msa0307500:intron 35.0%
TGCAATGTTATACATGTCAG+AGG - chr2_4:408461-408480 Msa0307500:five_prime_UTR 35.0%
AATGTTATACATGTCAGAGG+AGG - chr2_4:408464-408483 Msa0307500:five_prime_UTR 35.0%
TTATTGAGGATCATGTGTCT+TGG - chr2_4:408683-408702 Msa0307500:five_prime_UTR 35.0%
! ATATGATGGTTTTCGTTCTG+TGG - chr2_4:408775-408794 Msa0307500:five_prime_UTR 35.0%
ATCCAAAGCCAATTCAAGAA+TGG + chr2_4:408839-408858 None:intergenic 35.0%
TAGAGAAGGATGGATAGTAG+AGG + chr2_4:406925-406944 None:intergenic 40.0%
ATATCAAAGGGGGAATGATG+TGG + chr2_4:406950-406969 None:intergenic 40.0%
TTGTGTATGCTCATATGTGG+TGG + chr2_4:407112-407131 None:intergenic 40.0%
TGGAGTAAAGTTGGAGGAAT+GGG + chr2_4:407157-407176 None:intergenic 40.0%
! CAATTAAAAGGGTGTGTCAG+TGG + chr2_4:407177-407196 None:intergenic 40.0%
AACTCTTGAAGCCATAGTGA+AGG + chr2_4:407281-407300 None:intergenic 40.0%
AAGAACCCTTTCTGCTTGAA+GGG - chr2_4:407358-407377 Msa0307500:CDS 40.0%
CAGAAGTTTCATAACCAGCA+CGG + chr2_4:407405-407424 None:intergenic 40.0%
! ATGGAGTATCAGATCTGATC+TGG - chr2_4:407657-407676 Msa0307500:intron 40.0%
TTACAAGATTTCACTCCGCA+AGG + chr2_4:407812-407831 None:intergenic 40.0%
GCTTCTAATGCAAAGATCTG+GGG - chr2_4:407948-407967 Msa0307500:intron 40.0%
TTATGCTGGAATGCAAGCAA+AGG - chr2_4:408223-408242 Msa0307500:intron 40.0%
AAGTTGTGTTCTCAAAGCCA+GGG - chr2_4:408256-408275 Msa0307500:intron 40.0%
GTTATTCTCACCCGTTACAA+CGG - chr2_4:408411-408430 Msa0307500:exon 40.0%
ATTGGCAAACCTGTCATTAG+AGG + chr2_4:408442-408461 None:intergenic 40.0%
CCAATTCTTGCATTTGCTTG+AGG + chr2_4:408512-408531 None:intergenic 40.0%
CCTCAAGCAAATGCAAGAAT+TGG - chr2_4:408509-408528 Msa0307500:five_prime_UTR 40.0%
GCATAGAGCCATTCTTGAAT+TGG - chr2_4:408828-408847 Msa0307500:five_prime_UTR 40.0%
! AGCCATTCTTGAATTGGCTT+TGG - chr2_4:408834-408853 Msa0307500:five_prime_UTR 40.0%
TAGAGAAGGATGGATAGTAG+AGG + chr2_4:406925-406944 None:intergenic 40.0%
ATATCAAAGGGGGAATGATG+TGG + chr2_4:406950-406969 None:intergenic 40.0%
TTGTGTATGCTCATATGTGG+TGG + chr2_4:407112-407131 None:intergenic 40.0%
TGGAGTAAAGTTGGAGGAAT+GGG + chr2_4:407157-407176 None:intergenic 40.0%
! CAATTAAAAGGGTGTGTCAG+TGG + chr2_4:407177-407196 None:intergenic 40.0%
AACTCTTGAAGCCATAGTGA+AGG + chr2_4:407281-407300 None:intergenic 40.0%
AAGAACCCTTTCTGCTTGAA+GGG - chr2_4:407358-407377 Msa0307500:CDS 40.0%
CAGAAGTTTCATAACCAGCA+CGG + chr2_4:407405-407424 None:intergenic 40.0%
! ATGGAGTATCAGATCTGATC+TGG - chr2_4:407657-407676 Msa0307500:intron 40.0%
TTACAAGATTTCACTCCGCA+AGG + chr2_4:407812-407831 None:intergenic 40.0%
GCTTCTAATGCAAAGATCTG+GGG - chr2_4:407948-407967 Msa0307500:intron 40.0%
TTATGCTGGAATGCAAGCAA+AGG - chr2_4:408223-408242 Msa0307500:intron 40.0%
AAGTTGTGTTCTCAAAGCCA+GGG - chr2_4:408256-408275 Msa0307500:intron 40.0%
GTTATTCTCACCCGTTACAA+CGG - chr2_4:408411-408430 Msa0307500:exon 40.0%
ATTGGCAAACCTGTCATTAG+AGG + chr2_4:408442-408461 None:intergenic 40.0%
CCAATTCTTGCATTTGCTTG+AGG + chr2_4:408512-408531 None:intergenic 40.0%
CCTCAAGCAAATGCAAGAAT+TGG - chr2_4:408509-408528 Msa0307500:five_prime_UTR 40.0%
GCATAGAGCCATTCTTGAAT+TGG - chr2_4:408828-408847 Msa0307500:five_prime_UTR 40.0%
! AGCCATTCTTGAATTGGCTT+TGG - chr2_4:408834-408853 Msa0307500:five_prime_UTR 40.0%
TGATGTGGTGTAGAGAAGGA+TGG + chr2_4:406935-406954 None:intergenic 45.0%
GGAATGATGTGGTGTAGAGA+AGG + chr2_4:406939-406958 None:intergenic 45.0%
GCGTTGTGTATGCTCATATG+TGG + chr2_4:407115-407134 None:intergenic 45.0%
GTGGAGTAAAGTTGGAGGAA+TGG + chr2_4:407158-407177 None:intergenic 45.0%
TGTCAGTGGAGTAAAGTTGG+AGG + chr2_4:407163-407182 None:intergenic 45.0%
GTGTGTCAGTGGAGTAAAGT+TGG + chr2_4:407166-407185 None:intergenic 45.0%
TTTGTGTGTTCTTCCAGCGA+TGG - chr2_4:407315-407334 Msa0307500:CDS 45.0%
TGTTCTTCCAGCGATGGTTA+TGG - chr2_4:407321-407340 Msa0307500:CDS 45.0%
CTTCAAGCAGAAAGGGTTCT+TGG + chr2_4:407359-407378 None:intergenic 45.0%
CAAGAACCCTTTCTGCTTGA+AGG - chr2_4:407357-407376 Msa0307500:CDS 45.0%
TTTCATAACCAGCACGGCAA+GGG + chr2_4:407399-407418 None:intergenic 45.0%
TCTGCTATCACTCACATTGC+TGG - chr2_4:407421-407440 Msa0307500:CDS 45.0%
CTTGCATTCCAGCATAACCT+CGG + chr2_4:408220-408239 None:intergenic 45.0%
GAAGTTGTGTTCTCAAAGCC+AGG - chr2_4:408255-408274 Msa0307500:intron 45.0%
TAGAGGCAATGCCGTTGTAA+CGG + chr2_4:408425-408444 None:intergenic 45.0%
TAGTCTCAGTTCTGTTGCTG+TGG - chr2_4:408716-408735 Msa0307500:five_prime_UTR 45.0%
! GATGGTTTTCGTTCTGTGGA+TGG - chr2_4:408779-408798 Msa0307500:five_prime_UTR 45.0%
TGATGTGGTGTAGAGAAGGA+TGG + chr2_4:406935-406954 None:intergenic 45.0%
GGAATGATGTGGTGTAGAGA+AGG + chr2_4:406939-406958 None:intergenic 45.0%
GCGTTGTGTATGCTCATATG+TGG + chr2_4:407115-407134 None:intergenic 45.0%
GTGGAGTAAAGTTGGAGGAA+TGG + chr2_4:407158-407177 None:intergenic 45.0%
TGTCAGTGGAGTAAAGTTGG+AGG + chr2_4:407163-407182 None:intergenic 45.0%
GTGTGTCAGTGGAGTAAAGT+TGG + chr2_4:407166-407185 None:intergenic 45.0%
TTTGTGTGTTCTTCCAGCGA+TGG - chr2_4:407315-407334 Msa0307500:CDS 45.0%
TGTTCTTCCAGCGATGGTTA+TGG - chr2_4:407321-407340 Msa0307500:CDS 45.0%
CTTCAAGCAGAAAGGGTTCT+TGG + chr2_4:407359-407378 None:intergenic 45.0%
CAAGAACCCTTTCTGCTTGA+AGG - chr2_4:407357-407376 Msa0307500:CDS 45.0%
TTTCATAACCAGCACGGCAA+GGG + chr2_4:407399-407418 None:intergenic 45.0%
TCTGCTATCACTCACATTGC+TGG - chr2_4:407421-407440 Msa0307500:CDS 45.0%
CTTGCATTCCAGCATAACCT+CGG + chr2_4:408220-408239 None:intergenic 45.0%
GAAGTTGTGTTCTCAAAGCC+AGG - chr2_4:408255-408274 Msa0307500:intron 45.0%
TAGAGGCAATGCCGTTGTAA+CGG + chr2_4:408425-408444 None:intergenic 45.0%
TAGTCTCAGTTCTGTTGCTG+TGG - chr2_4:408716-408735 Msa0307500:five_prime_UTR 45.0%
! GATGGTTTTCGTTCTGTGGA+TGG - chr2_4:408779-408798 Msa0307500:five_prime_UTR 45.0%
AAAATTATCGGCGCGTCACG+CGG - chr2_4:406770-406789 Msa0307500:three_prime_UTR 50.0%
GTGCTACATGTGACTCCAAG+TGG + chr2_4:406845-406864 None:intergenic 50.0%
! GTTTTTGTAGCTGGCGCTGT+CGG + chr2_4:406867-406886 None:intergenic 50.0%
AGCAGAAAGGGTTCTTGGCT+GGG + chr2_4:407354-407373 None:intergenic 50.0%
AAGCAGAAAGGGTTCTTGGC+TGG + chr2_4:407355-407374 None:intergenic 50.0%
CACGACCCTTCAAGCAGAAA+GGG + chr2_4:407366-407385 None:intergenic 50.0%
ACACGACCCTTCAAGCAGAA+AGG + chr2_4:407367-407386 None:intergenic 50.0%
GTTTCATAACCAGCACGGCA+AGG + chr2_4:407400-407419 None:intergenic 50.0%
! TTTAGGGTTTAGGGAGGGTG+TGG - chr2_4:407600-407619 Msa0307500:intron 50.0%
! ATGAATCTGCTTAGGTGCCG+AGG - chr2_4:408200-408219 Msa0307500:intron 50.0%
TTTCGCTGCAACTACGCTCA+GGG + chr2_4:408365-408384 None:intergenic 50.0%
GGGTGAGAATAACACGTGCT+TGG + chr2_4:408404-408423 None:intergenic 50.0%
AGAGGCAATGCCGTTGTAAC+GGG + chr2_4:408424-408443 None:intergenic 50.0%
ACGGCATTGCCTCTAATGAC+AGG - chr2_4:408430-408449 Msa0307500:five_prime_UTR 50.0%
ATGTCAGAGGAGGTTGCTTC+TGG - chr2_4:408474-408493 Msa0307500:five_prime_UTR 50.0%
AAAATTATCGGCGCGTCACG+CGG - chr2_4:406770-406789 Msa0307500:three_prime_UTR 50.0%
GTGCTACATGTGACTCCAAG+TGG + chr2_4:406845-406864 None:intergenic 50.0%
! GTTTTTGTAGCTGGCGCTGT+CGG + chr2_4:406867-406886 None:intergenic 50.0%
AGCAGAAAGGGTTCTTGGCT+GGG + chr2_4:407354-407373 None:intergenic 50.0%
AAGCAGAAAGGGTTCTTGGC+TGG + chr2_4:407355-407374 None:intergenic 50.0%
CACGACCCTTCAAGCAGAAA+GGG + chr2_4:407366-407385 None:intergenic 50.0%
ACACGACCCTTCAAGCAGAA+AGG + chr2_4:407367-407386 None:intergenic 50.0%
GTTTCATAACCAGCACGGCA+AGG + chr2_4:407400-407419 None:intergenic 50.0%
! TTTAGGGTTTAGGGAGGGTG+TGG - chr2_4:407600-407619 Msa0307500:intron 50.0%
! ATGAATCTGCTTAGGTGCCG+AGG - chr2_4:408200-408219 Msa0307500:intron 50.0%
TTTCGCTGCAACTACGCTCA+GGG + chr2_4:408365-408384 None:intergenic 50.0%
GGGTGAGAATAACACGTGCT+TGG + chr2_4:408404-408423 None:intergenic 50.0%
AGAGGCAATGCCGTTGTAAC+GGG + chr2_4:408424-408443 None:intergenic 50.0%
ACGGCATTGCCTCTAATGAC+AGG - chr2_4:408430-408449 Msa0307500:five_prime_UTR 50.0%
ATGTCAGAGGAGGTTGCTTC+TGG - chr2_4:408474-408493 Msa0307500:five_prime_UTR 50.0%
CGAATGGCCATAACCATCGC+TGG + chr2_4:407331-407350 None:intergenic 55.0%
!! CTTAGGTGCCGAGGTTATGC+TGG - chr2_4:408209-408228 Msa0307500:intron 55.0%
CCAGTTGCATCAGTCTTCCC+TGG + chr2_4:408276-408295 None:intergenic 55.0%
CCAGGGAAGACTGATGCAAC+TGG - chr2_4:408273-408292 Msa0307500:CDS 55.0%
GGATCAGGATCGTGCAGATG+AGG - chr2_4:408314-408333 Msa0307500:CDS 55.0%
CTTTCGCTGCAACTACGCTC+AGG + chr2_4:408366-408385 None:intergenic 55.0%
!! AGTTGCAGCGAAAGAGCACC+TGG - chr2_4:408372-408391 Msa0307500:CDS 55.0%
!! GTTGCAGCGAAAGAGCACCT+GGG - chr2_4:408373-408392 Msa0307500:CDS 55.0%
CGAATGGCCATAACCATCGC+TGG + chr2_4:407331-407350 None:intergenic 55.0%
!! CTTAGGTGCCGAGGTTATGC+TGG - chr2_4:408209-408228 Msa0307500:intron 55.0%
CCAGTTGCATCAGTCTTCCC+TGG + chr2_4:408276-408295 None:intergenic 55.0%
CCAGGGAAGACTGATGCAAC+TGG - chr2_4:408273-408292 Msa0307500:CDS 55.0%
GGATCAGGATCGTGCAGATG+AGG - chr2_4:408314-408333 Msa0307500:CDS 55.0%
CTTTCGCTGCAACTACGCTC+AGG + chr2_4:408366-408385 None:intergenic 55.0%
!! AGTTGCAGCGAAAGAGCACC+TGG - chr2_4:408372-408391 Msa0307500:CDS 55.0%
!! GTTGCAGCGAAAGAGCACCT+GGG - chr2_4:408373-408392 Msa0307500:CDS 55.0%
AGGGTTCTTGGCTGGGCGAA+TGG + chr2_4:407347-407366 None:intergenic 60.0%
GTATGTGACCCTTGCCGTGC+TGG - chr2_4:407388-407407 Msa0307500:CDS 60.0%
! GGTTGCTTCTGGCTGTGCTG+AGG - chr2_4:408485-408504 Msa0307500:five_prime_UTR 60.0%
AGGGTTCTTGGCTGGGCGAA+TGG + chr2_4:407347-407366 None:intergenic 60.0%
GTATGTGACCCTTGCCGTGC+TGG - chr2_4:407388-407407 Msa0307500:CDS 60.0%
! GGTTGCTTCTGGCTGTGCTG+AGG - chr2_4:408485-408504 Msa0307500:five_prime_UTR 60.0%
ACACGTGCTTGGTCACGCCC+AGG + chr2_4:408393-408412 None:intergenic 65.0%
ACACGTGCTTGGTCACGCCC+AGG + chr2_4:408393-408412 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 406761 408951 406761 ID=Msa0307500;Name=Msa0307500
chr2_4 mRNA 406761 408951 406761 ID=Msa0307500-mRNA-1;Parent=Msa0307500;Name=Msa0307500-mRNA-1;_AED=0.01;_eAED=0.01;_QI=525|1|1|1|1|1|2|406|162
chr2_4 exon 406761 407498 406761 ID=Msa0307500-mRNA-1:exon:192;Parent=Msa0307500-mRNA-1
chr2_4 exon 408270 408951 408270 ID=Msa0307500-mRNA-1:exon:191;Parent=Msa0307500-mRNA-1
chr2_4 five_prime_UTR 408427 408951 408427 ID=Msa0307500-mRNA-1:five_prime_utr;Parent=Msa0307500-mRNA-1
chr2_4 CDS 408270 408426 408270 ID=Msa0307500-mRNA-1:cds;Parent=Msa0307500-mRNA-1
chr2_4 CDS 407167 407498 407167 ID=Msa0307500-mRNA-1:cds;Parent=Msa0307500-mRNA-1
chr2_4 three_prime_UTR 406761 407166 406761 ID=Msa0307500-mRNA-1:three_prime_utr;Parent=Msa0307500-mRNA-1
Gene Sequence

>Msa0307500

ATGGCTTCAAGAGTTGCTTTTTTGTTGTTTTTGTGTGTTCTTCCAGCGATGGTTATGGCCATTCGCCCAGCCAAGAACCCTTTCTGCTTGAAGGGTCGTGTTGTATGTGACCCTTGCCGTGCTGGTTATGAAACTTCTGCTATCACTCACATTGCTGGTGCCGAGGTTATGCTGGAATGCAAGCAAAGGAACAGCAACGAAGTTGTGTTCTCAAAGCCAGGGAAGACTGATGCAACTGGAGAATATACAATCTATGTGGATCAGGATCGTGCAGATGAGGTGTGTGATGTGAAGCTAATAAACAGCCCTGAGCGTAGTTGCAGCGAAAGAGCACCTGGGCGTGACCAAGCACGTGTTATTCTCACCCGTTACAACGGCATTGCCTCTAATGACAGGTTTGCCAATGCAATGTTATACATGTCAGAGGAGGTTGCTTCTGGCTGTGCTGAGGTCCTCAAGCAAATGCAAGAATTGGATGAAGAGAATTAA
Protein sequence

>Msa0307500

MASRVAFLLFLCVLPAMVMAIRPAKNPFCLKGRVVCDPCRAGYETSAITHIAGAEVMLECKQRNSNEVVFSKPGKTDATGEYTIYVDQDRADEVCDVKLINSPERSCSERAPGRDQARVILTRYNGIASNDRFANAMLYMSEEVASGCAEVLKQMQELDEEN*