AlfalfaGEDB | Msa0307660

M. sativa cultivar zhongmu-4 / Msa0307660

KEGG: K09338
GO: -
PFAM: PF00046.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0307660	XP_013465933.1	93.015	272	17	2	1	271	1	271	0.0	511

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0307660	sp\|P46604\|HAT22_ARATH	49.398	249	111	7	1	245	1	238	5.85e-60	194

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0307660	TF	HB-HD-ZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017680	Msa0307660	0.818468	1.929385e-52	-8.615850e-47
Msa0098030	Msa0307660	0.838108	3.498298e-57	-8.615850e-47
Msa0307660	Msa0792380	0.976733	2.150595e-142	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0307660	MtrunA17_Chr1g0151971	93.015	272	17	2	1	271	1	271	0.0	511
Msa0307660	MtrunA17_Chr4g0054421	72.308	130	32	2	118	245	112	239	3.12e-60	192
Msa0307660	MtrunA17_Chr5g0400781	49.784	231	101	5	49	272	41	263	2.93e-59	189
Msa0307660	MtrunA17_Chr1g0175651	70.079	127	36	1	119	245	142	266	9.93e-58	186
Msa0307660	MtrunA17_Chr7g0258831	69.919	123	35	1	124	246	153	273	1.65e-56	183
Msa0307660	MtrunA17_Chr3g0135331	48.473	262	111	10	28	271	34	289	2.54e-54	177
Msa0307660	MtrunA17_Chr5g0399491	51.892	185	75	5	89	271	96	268	2.10e-53	175
Msa0307660	MtrunA17_Chr4g0055851	52.778	180	68	5	89	267	85	248	1.77e-52	172
Msa0307660	MtrunA17_Chr2g0308611	71.552	116	31	1	130	245	199	312	2.72e-52	173
Msa0307660	MtrunA17_Chr8g0335131	69.565	115	32	2	130	243	145	257	8.87e-50	166

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0307660	AT4G37790.1	49.398	249	111	7	1	245	1	238	5.95e-61	194
Msa0307660	AT5G06710.1	56.425	179	63	5	89	258	140	312	1.45e-58	190
Msa0307660	AT2G22800.1	45.968	248	108	6	1	245	1	225	2.90e-54	177
Msa0307660	AT4G16780.1	59.589	146	48	4	124	267	121	257	2.00e-51	170
Msa0307660	AT3G60390.1	66.667	123	40	1	121	243	152	273	9.19e-51	169
Msa0307660	AT5G47370.1	46.544	217	91	7	65	270	54	256	3.06e-49	164
Msa0307660	AT2G44910.1	68.376	117	34	2	130	245	162	276	9.60e-49	164
Msa0307660	AT4G17460.1	40.408	245	132	6	16	247	7	250	3.21e-46	156
Msa0307660	AT2G01430.1	46.584	161	73	5	94	245	93	249	8.41e-39	137
Msa0307660	AT1G70920.1	46.667	150	67	2	106	253	44	182	1.97e-34	124

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Find 66 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAACAATCAAAAGTTT+TGG	0.344899	2_4:+644837	Msa0307660:CDS
AAAACCTTGAAGAAAATTAT+TGG	0.218253	2_4:-644381	None:intergenic
AAATCCATCAAAATGCATTA+AGG	0.347365	2_4:+644546	Msa0307660:CDS
AAATTATTGGAGGAGCTTGT+AGG	0.401643	2_4:-644368	None:intergenic
AAATTCCACCAGCATCAGGA+AGG	0.618266	2_4:+644714	Msa0307660:CDS
AAGCAATGTTGTTGAGGGTA+AGG	0.485816	2_4:-646419	None:intergenic
AAGGTGCCAATTACAAGGAT+AGG	0.527337	2_4:+644769	Msa0307660:CDS
AAGTTGAAGCCATGTGTTGG+AGG	0.556547	2_4:-644446	None:intergenic
ACAACATTCAACATCAAAGA+CGG	0.613762	2_4:+644630	Msa0307660:CDS
ACATTTCCTATCCTTGTAAT+TGG	0.209590	2_4:-644775	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATTATTAGGATTTAGCG+GGG	-	chr2_4:644219-644238	None:intergenic	30.0%
GAAATAGGAAATGATAGGAA+TGG	-	chr2_4:644291-644310	None:intergenic	30.0%
GATGTGAAATAGGAAATGAT+AGG	-	chr2_4:644296-644315	None:intergenic	30.0%
AAAAAGAGAGGATGTGAAAT+AGG	-	chr2_4:644306-644325	None:intergenic	30.0%
TTGTAGGTTGCAATATTCTT+TGG	-	chr2_4:644355-644374	None:intergenic	30.0%
ACCTTGAAGAAAATTATTGG+AGG	-	chr2_4:644381-644400	None:intergenic	30.0%
GATCATCAAAATGATCATGA+AGG	-	chr2_4:644492-644511	None:intergenic	30.0%
ACAACATTCAACATCAAAGA+CGG	+	chr2_4:644630-644649	Msa0307660:CDS	30.0%
ACATTTCCTATCCTTGTAAT+TGG	-	chr2_4:644778-644797	None:intergenic	30.0%
TTAGAATTAGATCTTGGTCT+TGG	+	chr2_4:645148-645167	Msa0307660:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	644208	646699	644208	ID=Msa0307660;Name=Msa0307660
chr2_4	mRNA	644208	646699	644208	ID=Msa0307660-mRNA-1;Parent=Msa0307660;Name=Msa0307660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=222\|1\|1\|1\|1\|1\|3\|213\|274
chr2_4	exon	644208	644894	644208	ID=Msa0307660-mRNA-1:exon:296;Parent=Msa0307660-mRNA-1
chr2_4	exon	645571	645650	645571	ID=Msa0307660-mRNA-1:exon:297;Parent=Msa0307660-mRNA-1
chr2_4	exon	646207	646699	646207	ID=Msa0307660-mRNA-1:exon:298;Parent=Msa0307660-mRNA-1
chr2_4	five_prime_UTR	644208	644429	644208	ID=Msa0307660-mRNA-1:five_prime_utr;Parent=Msa0307660-mRNA-1
chr2_4	CDS	644430	644894	644430	ID=Msa0307660-mRNA-1:cds;Parent=Msa0307660-mRNA-1
chr2_4	CDS	645571	645650	645571	ID=Msa0307660-mRNA-1:cds;Parent=Msa0307660-mRNA-1
chr2_4	CDS	646207	646486	646207	ID=Msa0307660-mRNA-1:cds;Parent=Msa0307660-mRNA-1
chr2_4	three_prime_UTR	646487	646699	646487	ID=Msa0307660-mRNA-1:three_prime_utr;Parent=Msa0307660-mRNA-1

Gene Sequence

>Msa0307660

ATGAATTTTGATGATACCTCCAACACATGGCTTCAACTTGATCTTGATCTAGGTCTTACCCTTCATGATCATTTTGATGATCATGGGAAGAGAGAAAGCAAGAAGAAAAGAGTGAAAAATCCATCAAAATGCATTAAGGCTTATCCAATGCTTACATTAGGACCATCAAAGGAAGAGAGTGAAGAAAATACTAAAATTCAACAACATTCAACATCAAAGACGGAATCTTGTGATCAAAATTTACCTACTCAATTTACTTCTGCAAGCATAGAGTCTTCATTTTCAAATTCCACCAGCATCAGGAAGGAGAGTGGTGAGGAATTTGAGGTAGAGATAGAAAAGGTGCCAATTACAAGGATAGGAAATGTTGATAAAGATGGTAGCCCAAGGAAGAAACTTAGGCTCACAAAACAACAATCAAAAGTTTTGGAGGAAAACTTCAAAGAGCATTCTACTCTTAATCCGAAGCAAAAGCAAGCATTAGCAAATAAGCTGAATCTGCAGGCTAGACAAGTAGAGGTGTGGTTTCAAAATCGGAGAGCCAGAACAAAGCTGAAACAAACGGAATCTGATTGTGAAATACTGAAGAAGTGTTGCGAATCTCTAACAGAGGAGAACAAGAAGTTGCAAAAGGAGCTACAAGAACTAAAGTCAATGCAAACAGTAGCAGCAGCACCCTTTTATATGAAGATTCCAGCAGCAACACTTACCATCTGCCCTTCGTGCGAGACAATTTGTGGTGGCAACAACAATGGCTCCTTACCCTCAACAACATTGCTTATTGGCTCAAAGAATCATCATTTCCATAAAAACTTTTTGGTTTGA

Protein sequence

>Msa0307660

MNFDDTSNTWLQLDLDLGLTLHDHFDDHGKRESKKKRVKNPSKCIKAYPMLTLGPSKEESEENTKIQQHSTSKTESCDQNLPTQFTSASIESSFSNSTSIRKESGEEFEVEIEKVPITRIGNVDKDGSPRKKLRLTKQQSKVLEENFKEHSTLNPKQKQALANKLNLQARQVEVWFQNRRARTKLKQTESDCEILKKCCESLTEENKKLQKELQELKSMQTVAAAPFYMKIPAATLTICPSCETICGGNNNGSLPSTTLLIGSKNHHFHKNFLV*