AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0308420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308420 MtrunA17_Chr2g0293811 99.185 368 3 0 1 368 1 368 0.0 750
Msa0308420 MtrunA17_Chr1g0194191 57.364 387 146 2 1 368 1 387 3.95e-157 446
Msa0308420 MtrunA17_Chr5g0407161 50.649 308 137 3 60 366 84 377 2.13e-97 294
Msa0308420 MtrunA17_Chr4g0068971 84.277 159 25 0 210 368 110 268 1.14e-96 288
Msa0308420 MtrunA17_Chr4g0068971 58.559 111 33 3 1 98 1 111 1.64e-31 120
Msa0308420 MtrunA17_Chr3g0144111 49.488 293 114 2 74 366 1 259 2.25e-96 287
Msa0308420 MtrunA17_Chr4g0065141 45.634 355 164 8 22 366 48 383 1.43e-89 274
Msa0308420 MtrunA17_Chr3g0138281 44.214 337 172 6 34 367 55 378 1.87e-88 271
Msa0308420 MtrunA17_Chr1g0150451 32.971 276 151 8 59 329 75 321 4.44e-39 142
Msa0308420 MtrunA17_Chr5g0430481 34.137 249 141 5 89 331 143 374 2.35e-38 141
Msa0308420 MtrunA17_Chr3g0136711 30.945 307 162 9 36 329 9 278 5.13e-38 138
Msa0308420 MtrunA17_Chr3g0127091 32.143 364 179 14 9 328 7 346 1.39e-37 139
Msa0308420 MtrunA17_Chr1g0212961 35.897 234 124 7 99 329 125 335 1.12e-35 133
Msa0308420 MtrunA17_Chr4g0050381 33.755 237 129 7 99 329 60 274 1.02e-34 129
Msa0308420 MtrunA17_Chr3g0115851 34.177 237 128 7 99 329 65 279 1.39e-34 128
Msa0308420 MtrunA17_Chr1g0171571 33.333 303 145 11 56 336 382 649 9.14e-34 132
Msa0308420 MtrunA17_Chr4g0001761 30.462 325 151 9 60 329 22 326 1.97e-33 127
Msa0308420 MtrunA17_Chr2g0279151 32.911 237 131 7 99 329 64 278 5.76e-33 124
Msa0308420 MtrunA17_Chr1g0150071 29.655 290 135 5 59 298 21 291 8.51e-33 125
Msa0308420 MtrunA17_Chr3g0105371 30.545 275 148 7 69 331 95 338 1.02e-32 125
Msa0308420 MtrunA17_Chr6g0483411 30.380 316 181 10 38 331 20 318 2.31e-32 123
Msa0308420 MtrunA17_Chr3g0136811 29.553 291 134 6 59 298 21 291 2.64e-32 124
Msa0308420 MtrunA17_Chr5g0426141 32.061 262 142 8 74 329 48 279 1.31e-31 120
Msa0308420 MtrunA17_Chr4g0051661 35.021 237 125 7 99 329 182 395 3.11e-31 122
Msa0308420 MtrunA17_Chr3g0126511 35.537 242 116 9 100 329 125 338 2.58e-30 119
Msa0308420 MtrunA17_Chr8g0360351 31.224 237 135 7 99 329 65 279 5.15e-30 116
Msa0308420 MtrunA17_Chr3g0105351 29.630 270 157 7 69 331 71 314 5.69e-30 117
Msa0308420 MtrunA17_Chr2g0317021 31.381 239 136 7 99 331 116 332 7.07e-30 117
Msa0308420 MtrunA17_Chr3g0112291 31.461 267 136 7 97 329 120 373 1.26e-29 117
Msa0308420 MtrunA17_Chr4g0070871 30.435 276 160 9 87 337 241 509 3.71e-29 117
Msa0308420 MtrunA17_Chr1g0150781 30.189 265 151 5 95 331 285 543 6.39e-29 117
Msa0308420 MtrunA17_Chr1g0158781 32.394 284 159 10 63 331 114 379 1.76e-28 114
Msa0308420 MtrunA17_Chr3g0135961 29.740 269 141 7 100 333 286 541 3.43e-26 109
Msa0308420 MtrunA17_Chr5g0396731 31.111 225 120 6 93 301 100 305 6.79e-26 106
Msa0308420 MtrunA17_Chr2g0299981 29.242 277 147 8 100 333 267 537 3.19e-25 107
Msa0308420 MtrunA17_Chr5g0435721 31.797 217 116 7 100 299 223 424 5.63e-24 102
Msa0308420 MtrunA17_Chr8g0389921 28.076 317 196 8 50 342 137 445 1.37e-22 98.6
Msa0308420 MtrunA17_Chr7g0263281 29.749 279 149 9 100 333 71 347 3.10e-20 90.9
Msa0308420 MtrunA17_Chr1g0213351 29.553 291 130 10 100 331 80 354 4.90e-20 90.5
Msa0308420 MtrunA17_Chr1g0207571 29.167 288 150 9 94 331 54 337 1.10e-19 89.4
Msa0308420 MtrunA17_Chr4g0066371 26.429 280 143 8 100 336 86 345 1.58e-19 89.4
Msa0308420 MtrunA17_Chr8g0371321 26.316 285 147 8 100 331 79 353 2.25e-18 85.9
Msa0308420 MtrunA17_Chr7g0243571 27.974 311 149 12 53 317 62 343 5.59e-18 85.1
Msa0308420 MtrunA17_Chr2g0295401 25.905 359 196 12 25 345 34 360 9.58e-18 83.6
Msa0308420 MtrunA17_Chr1g0211251 26.573 286 145 10 100 331 81 355 1.45e-17 83.6
Msa0308420 MtrunA17_Chr2g0286951 27.106 273 136 8 100 329 87 339 1.85e-17 83.2
Msa0308420 MtrunA17_Chr4g0067711 26.220 328 178 11 25 317 33 331 6.21e-17 81.3
Msa0308420 MtrunA17_Chr7g0249421 34.043 188 95 7 127 294 166 344 1.13e-16 81.3
Msa0308420 MtrunA17_Chr2g0294291 26.502 283 152 9 100 332 75 351 3.53e-16 79.0
Msa0308420 MtrunA17_Chr7g0250061 25.000 288 147 10 100 331 76 350 1.37e-15 77.4
Msa0308420 MtrunA17_Chr4g0004971 27.757 263 139 10 100 323 78 328 1.38e-15 77.0
Msa0308420 MtrunA17_Chr1g0175381 27.519 258 130 11 98 316 77 316 1.83e-15 77.0
Msa0308420 MtrunA17_Chr1g0151781 26.638 229 128 8 99 294 67 288 2.42e-15 76.3
Msa0308420 MtrunA17_Chr7g0221691 29.520 271 128 11 98 316 127 386 2.74e-15 77.0
Msa0308420 MtrunA17_Chr7g0256771 29.218 243 113 9 100 299 70 296 5.30e-15 75.1
Msa0308420 MtrunA17_Chr3g0089721 26.754 228 138 6 100 299 68 294 1.21e-14 74.3
Msa0308420 MtrunA17_Chr1g0187481 24.255 235 143 8 96 329 75 275 1.37e-14 73.2
Msa0308420 MtrunA17_Chr3g0089711 26.087 230 141 6 98 299 66 294 3.35e-14 73.2
Msa0308420 MtrunA17_Chr5g0394181 28.148 270 158 12 93 341 351 605 3.51e-14 73.9
Msa0308420 MtrunA17_Chr1g0181901 27.350 234 119 8 100 294 76 297 7.36e-14 72.4
Msa0308420 MtrunA17_Chr7g0256751 28.270 237 123 10 100 299 69 295 1.03e-13 72.0
Msa0308420 MtrunA17_Chr7g0272061 28.649 185 102 5 170 334 183 357 4.85e-13 69.7
Msa0308420 MtrunA17_Chr1g0192421 27.309 249 140 10 100 316 89 328 1.05e-12 68.6
Msa0308420 MtrunA17_Chr1g0192471 29.921 254 136 11 96 329 64 295 1.44e-12 68.6
Msa0308420 MtrunA17_Chr7g0271971 29.200 250 143 11 96 329 64 295 1.66e-12 68.2
Msa0308420 MtrunA17_Chr1g0153671 26.766 269 161 11 99 350 64 313 1.67e-11 65.1
Msa0308420 MtrunA17_Chr7g0225501 30.288 208 121 8 96 288 82 280 2.28e-11 65.1
Msa0308420 MtrunA17_Chr4g0022171 25.000 332 173 14 55 331 58 368 4.34e-11 63.9
Msa0308420 MtrunA17_Chr4g0063051 27.706 231 140 8 97 317 58 271 7.89e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308420 AT1G48040.1 56.477 386 135 8 1 365 7 380 1.33e-142 410
Msa0308420 AT3G62260.1 53.786 383 159 5 1 366 1 382 2.62e-136 394
Msa0308420 AT3G62260.2 53.385 384 160 5 1 366 1 383 3.44e-136 394
Msa0308420 AT3G17250.1 54.737 380 136 9 12 366 53 421 1.06e-132 386
Msa0308420 AT3G62260.3 59.524 294 117 2 74 366 1 293 6.29e-124 359
Msa0308420 AT2G33700.1 50.633 316 134 4 56 366 79 377 8.39e-104 311
Msa0308420 AT3G51470.2 49.842 317 139 4 50 365 17 314 1.19e-98 295
Msa0308420 AT3G51470.1 49.842 317 139 4 50 365 61 358 3.25e-98 296
Msa0308420 AT2G25620.1 43.202 331 171 6 8 331 36 356 5.38e-82 255
Msa0308420 AT2G25620.2 43.202 331 171 6 8 331 36 356 5.38e-82 255
Msa0308420 AT4G31750.2 33.099 284 140 9 59 329 31 277 1.47e-39 142
Msa0308420 AT4G31750.1 33.099 284 140 9 59 329 31 277 2.04e-39 142
Msa0308420 AT4G31750.3 33.099 284 140 9 59 329 31 277 2.04e-39 142
Msa0308420 AT1G43900.1 34.397 282 142 10 57 329 119 366 3.75e-39 143
Msa0308420 AT5G24940.1 34.058 276 148 8 59 329 31 277 8.56e-39 144
Msa0308420 AT2G20630.2 33.603 247 136 7 99 339 62 286 9.16e-39 140
Msa0308420 AT5G10740.1 33.451 284 139 10 59 329 31 277 3.29e-38 140
Msa0308420 AT5G10740.2 33.451 284 139 10 59 329 31 277 3.29e-38 140
Msa0308420 AT2G20630.1 33.755 237 129 7 99 329 62 276 6.53e-38 137
Msa0308420 AT1G67820.2 34.146 246 134 6 69 310 92 313 5.75e-37 138
Msa0308420 AT1G78200.5 35.443 237 128 7 99 329 18 235 1.04e-36 133
Msa0308420 AT1G78200.4 35.443 237 128 7 99 329 18 235 1.04e-36 133
Msa0308420 AT1G67820.1 33.730 252 139 6 63 310 122 349 1.68e-36 137
Msa0308420 AT1G78200.1 35.021 237 129 7 99 329 63 280 2.51e-36 133
Msa0308420 AT1G78200.3 35.021 237 129 7 99 329 63 280 2.51e-36 133
Msa0308420 AT1G78200.2 35.021 237 129 7 99 329 63 280 2.51e-36 133
Msa0308420 AT1G07160.1 31.481 270 155 6 69 331 130 376 4.60e-36 135
Msa0308420 AT4G28400.3 33.755 237 129 7 99 329 44 258 5.97e-36 132
Msa0308420 AT4G28400.1 33.755 237 129 7 99 329 66 280 1.14e-35 132
Msa0308420 AT5G53140.1 34.711 242 118 8 100 329 132 345 3.89e-35 133
Msa0308420 AT5G53140.2 34.711 242 118 8 100 329 132 345 3.89e-35 133
Msa0308420 AT2G40180.1 33.829 269 153 8 69 331 136 385 1.88e-34 131
Msa0308420 AT2G25070.1 30.000 320 162 9 59 329 21 327 3.79e-34 129
Msa0308420 AT2G25070.2 30.000 320 162 9 59 329 21 327 3.79e-34 129
Msa0308420 AT1G22280.3 33.750 240 131 8 99 329 64 284 4.94e-33 125
Msa0308420 AT2G30020.1 33.891 239 137 5 99 331 169 392 8.10e-33 127
Msa0308420 AT1G22280.1 33.755 237 129 7 99 329 64 278 3.25e-32 122
Msa0308420 AT4G31860.1 29.245 318 167 7 59 329 21 327 9.83e-32 123
Msa0308420 AT1G18030.1 31.429 315 167 11 41 334 65 351 1.73e-31 122
Msa0308420 AT4G31860.3 30.742 283 143 6 59 299 21 292 5.55e-31 119
Msa0308420 AT1G07430.1 33.977 259 134 8 61 300 120 360 8.94e-31 122
Msa0308420 AT2G40860.1 32.226 301 160 9 51 336 382 653 1.27e-30 123
Msa0308420 AT2G40860.4 32.226 301 160 9 51 336 284 555 1.70e-30 122
Msa0308420 AT1G34750.2 33.054 239 132 7 99 331 65 281 1.78e-30 118
Msa0308420 AT1G34750.1 33.054 239 132 7 99 331 65 281 1.78e-30 118
Msa0308420 AT1G34750.3 33.054 239 132 7 99 331 65 281 1.78e-30 118
Msa0308420 AT3G15260.2 32.068 237 133 8 99 329 72 286 5.41e-30 117
Msa0308420 AT3G15260.1 32.068 237 133 8 99 329 72 286 5.41e-30 117
Msa0308420 AT5G51760.2 30.466 279 142 8 97 331 111 381 8.14e-29 115
Msa0308420 AT5G51760.1 30.325 277 141 8 97 329 141 409 1.10e-28 115
Msa0308420 AT3G11410.1 28.674 279 164 7 41 299 84 347 3.98e-28 114
Msa0308420 AT4G28400.2 34.896 192 101 6 99 284 66 239 4.90e-27 108
Msa0308420 AT4G31860.2 30.451 266 132 6 59 282 21 275 6.09e-27 108
Msa0308420 AT2G29380.1 31.429 315 167 12 45 329 63 358 1.68e-26 108
Msa0308420 AT2G40860.2 31.786 280 147 9 51 315 382 632 1.98e-26 111
Msa0308420 AT4G26080.1 30.038 263 141 10 100 331 172 422 3.34e-26 109
Msa0308420 AT4G27800.1 30.389 283 154 9 99 346 87 361 3.84e-25 105
Msa0308420 AT5G59220.1 29.934 304 176 10 23 308 72 356 1.04e-24 104
Msa0308420 AT5G57050.2 27.068 266 158 6 100 337 120 377 2.14e-24 103
Msa0308420 AT5G57050.1 27.376 263 155 6 100 334 160 414 2.41e-24 103
Msa0308420 AT1G17550.1 29.021 286 147 9 92 333 230 503 3.65e-24 103
Msa0308420 AT1G17550.2 29.021 286 147 9 92 333 230 503 3.65e-24 103
Msa0308420 AT2G34740.1 27.500 240 144 8 99 331 118 334 6.84e-24 101
Msa0308420 AT1G18030.2 30.566 265 136 10 40 284 64 300 1.57e-23 99.8
Msa0308420 AT3G16800.2 32.509 283 142 13 65 318 66 328 3.79e-23 99.4
Msa0308420 AT3G16800.1 32.509 283 142 13 65 318 66 328 3.79e-23 99.4
Msa0308420 AT3G16800.3 32.509 283 142 13 65 318 66 328 3.79e-23 99.4
Msa0308420 AT3G16800.4 39.153 189 86 9 150 318 54 233 4.64e-23 97.4
Msa0308420 AT3G16800.6 39.153 189 86 9 150 318 54 233 4.64e-23 97.4
Msa0308420 AT1G72770.4 29.749 279 138 10 100 333 238 503 8.28e-23 100
Msa0308420 AT1G72770.1 29.749 279 138 10 100 333 238 503 8.28e-23 100
Msa0308420 AT1G72770.3 29.749 279 138 10 100 333 238 503 8.28e-23 100
Msa0308420 AT3G06270.1 28.205 273 133 8 100 329 88 340 5.26e-22 96.3
Msa0308420 AT4G27800.2 31.225 253 133 8 99 316 87 333 9.74e-22 95.1
Msa0308420 AT4G27800.3 31.148 244 127 8 99 307 87 324 8.05e-21 92.4
Msa0308420 AT3G55050.1 29.655 290 139 12 98 333 82 360 1.89e-19 89.4
Msa0308420 AT3G55050.2 29.655 290 139 12 98 333 82 360 1.89e-19 89.4
Msa0308420 AT3G51370.1 25.253 297 159 8 100 343 79 365 2.48e-19 89.0
Msa0308420 AT3G51370.3 25.253 297 159 8 100 343 79 365 2.48e-19 89.0
Msa0308420 AT2G34740.3 29.442 197 113 7 99 288 114 291 3.24e-19 87.4
Msa0308420 AT2G34740.2 29.442 197 113 7 99 288 132 309 3.52e-19 87.8
Msa0308420 AT1G34750.4 31.771 192 107 6 99 284 65 238 4.42e-19 85.9
Msa0308420 AT5G02760.1 27.875 287 140 12 100 332 72 345 6.85e-19 87.4
Msa0308420 AT5G66080.1 27.336 289 139 11 100 331 82 356 1.89e-18 86.3
Msa0308420 AT3G02750.2 34.615 182 95 6 132 294 177 353 2.79e-18 86.7
Msa0308420 AT3G02750.1 34.615 182 95 6 132 294 177 353 2.79e-18 86.7
Msa0308420 AT5G06750.3 26.408 284 155 7 98 331 80 359 3.56e-18 85.5
Msa0308420 AT5G06750.1 26.408 284 155 7 98 331 80 359 3.56e-18 85.5
Msa0308420 AT2G40860.3 32.500 240 121 8 51 275 382 595 4.61e-18 86.3
Msa0308420 AT4G38520.2 27.046 281 146 7 100 329 81 353 7.34e-18 84.7
Msa0308420 AT4G38520.1 27.046 281 146 7 100 329 81 353 7.34e-18 84.7
Msa0308420 AT1G68410.4 31.613 310 166 14 52 342 31 313 2.62e-17 83.2
Msa0308420 AT1G68410.3 31.613 310 166 14 52 342 31 313 2.62e-17 83.2
Msa0308420 AT1G68410.1 31.613 310 166 14 52 342 31 313 2.62e-17 83.2
Msa0308420 AT1G68410.2 31.613 310 166 14 52 342 31 313 2.62e-17 83.2
Msa0308420 AT3G12620.2 29.630 297 126 12 98 331 81 357 2.69e-17 82.8
Msa0308420 AT3G12620.1 29.630 297 126 12 98 331 81 357 2.69e-17 82.8
Msa0308420 AT3G17090.1 26.736 288 144 9 100 332 84 359 5.51e-17 82.0
Msa0308420 AT5G36250.1 32.020 203 110 8 134 317 170 363 1.32e-16 81.3
Msa0308420 AT4G33920.1 27.817 284 151 8 98 331 59 338 1.39e-16 80.9
Msa0308420 AT1G47380.1 28.676 272 158 10 96 350 60 312 3.95e-16 79.7
Msa0308420 AT5G27930.1 28.044 271 144 10 100 329 92 352 1.01e-15 78.2
Msa0308420 AT5G27930.2 28.044 271 144 10 100 329 92 352 1.01e-15 78.2
Msa0308420 AT3G05640.2 28.517 263 150 9 100 334 93 345 1.87e-15 77.4
Msa0308420 AT3G05640.1 28.517 263 150 9 100 334 93 345 1.87e-15 77.4
Msa0308420 AT3G05640.3 28.517 263 150 9 100 334 93 345 1.87e-15 77.4
Msa0308420 AT1G79630.6 32.258 186 92 6 170 331 112 287 2.26e-15 77.4
Msa0308420 AT1G79630.3 32.258 186 92 6 170 331 112 287 2.26e-15 77.4
Msa0308420 AT1G79630.4 32.258 186 92 6 170 331 112 287 2.26e-15 77.4
Msa0308420 AT1G79630.5 32.258 186 92 6 170 331 112 287 2.26e-15 77.4
Msa0308420 AT1G79630.2 32.258 186 92 6 170 331 152 327 2.66e-15 77.4
Msa0308420 AT1G79630.7 32.258 186 92 6 170 331 152 327 2.66e-15 77.4
Msa0308420 AT1G79630.8 32.258 186 92 6 170 331 218 393 4.00e-15 77.0
Msa0308420 AT1G79630.1 32.258 186 92 6 170 331 222 397 4.88e-15 76.6
Msa0308420 AT4G08260.1 28.936 235 121 7 99 331 18 208 1.30e-14 72.8
Msa0308420 AT1G16220.1 31.720 186 93 6 170 331 199 374 1.76e-14 75.1
Msa0308420 AT3G16560.1 29.880 251 118 8 100 294 175 423 1.80e-14 75.1
Msa0308420 AT3G16560.4 29.880 251 118 8 100 294 175 423 1.80e-14 75.1
Msa0308420 AT3G16560.2 29.880 251 118 8 100 294 175 423 1.80e-14 75.1
Msa0308420 AT3G16560.3 29.880 251 118 8 100 294 175 423 2.08e-14 74.7
Msa0308420 AT3G51370.2 25.000 276 145 8 122 343 13 280 3.21e-14 72.8
Msa0308420 AT5G06750.2 28.837 215 101 6 167 331 65 277 9.22e-14 71.6
Msa0308420 AT5G59220.2 27.731 238 141 8 23 242 72 296 2.17e-13 70.5
Msa0308420 AT5G57050.3 27.815 151 101 4 100 244 160 308 3.37e-13 70.1
Msa0308420 AT5G01700.1 28.413 271 146 8 100 332 29 289 8.14e-13 69.3
Msa0308420 AT4G32950.1 26.022 269 161 10 87 332 62 315 1.09e-12 68.6
Msa0308420 AT5G01700.2 28.413 271 146 8 100 332 78 338 1.52e-12 68.6
Msa0308420 AT4G33920.2 30.233 215 98 7 167 331 43 255 1.54e-12 67.8
Msa0308420 AT4G33920.3 30.233 215 98 7 167 331 13 225 1.78e-12 67.4
Msa0308420 AT5G66080.2 26.210 248 116 9 140 331 16 252 2.73e-12 67.0
Msa0308420 AT5G26010.1 26.606 218 126 7 102 294 75 283 1.02e-11 65.9

Find 102 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATGCCTTCCAAGTATA+AGG 0.238260 2_4:-2651221 None:intergenic
GAATTTCTCGATTTCGATTT+GGG 0.241434 2_4:-2649875 None:intergenic
CACTCGTGCCCTTGGCGATT+GGG 0.294126 2_4:+2651404 Msa0308420:CDS
TGAATTTCTCGATTTCGATT+TGG 0.294260 2_4:-2649876 None:intergenic
TTCCATTCCTACAAAACTTC+TGG 0.325777 2_4:-2651881 None:intergenic
TCTGCTCATCGGCCAATAAA+AGG 0.328870 2_4:-2651161 None:intergenic
TACCGCTGAGCCCGATGTTC+AGG 0.329190 2_4:+2651461 Msa0308420:CDS
TCATTGGGTGTGATGGCATC+TGG 0.329824 2_4:+2651517 Msa0308420:CDS
TCACTCGTGCCCTTGGCGAT+TGG 0.336784 2_4:+2651403 Msa0308420:CDS
TATACTTGGAAGGCATTTGC+TGG 0.338710 2_4:+2651224 Msa0308420:CDS
TAGGAATGGAAGAGGATAGA+TGG 0.345588 2_4:+2651893 Msa0308420:three_prime_UTR
TCTTCCACTCTCTTGCGTTC+TGG 0.346941 2_4:-2651333 None:intergenic
AACTTTATAAATTCAGTGTT+TGG 0.358900 2_4:-2652233 None:intergenic
AGCTACCAGAACGAACCTTT+GGG 0.376250 2_4:-2650864 None:intergenic
CAGAATTGATTAATGTTTGT+AGG 0.407432 2_4:+2651835 Msa0308420:three_prime_UTR
CAGCTACCAGAACGAACCTT+TGG 0.414252 2_4:-2650865 None:intergenic
GAAGACGAGTTCTTGATCAT+TGG 0.416556 2_4:+2651501 Msa0308420:CDS
TGCTTCTTTGACAAGCTCTC+TGG 0.424480 2_4:-2651616 None:intergenic
GGATAGATGGAGAGAGTTGG+TGG 0.428502 2_4:+2651906 Msa0308420:three_prime_UTR
AAGCTCTGAATCTTCGCCTA+TGG 0.436917 2_4:-2651722 None:intergenic
AAGACGAGTTCTTGATCATT+GGG 0.439912 2_4:+2651502 Msa0308420:CDS
TCTGGTAGCTGTGCTGATAT+TGG 0.447446 2_4:+2650877 Msa0308420:CDS
CATTTGCTGGTTGCTAATGC+TGG 0.454775 2_4:+2651237 Msa0308420:CDS
AGCTCTGAATCTTCGCCTAT+GGG 0.455149 2_4:-2651721 None:intergenic
AATGTAGCAAAAGCTAGTTT+TGG 0.455645 2_4:+2651997 Msa0308420:three_prime_UTR
GCACTGACTGCCCTTATACT+TGG 0.459789 2_4:+2651210 Msa0308420:CDS
CTATCTGTCACTCGTGCCCT+TGG 0.462680 2_4:+2651396 Msa0308420:CDS
GAGAGAGGTTTCAGTTGAAA+CGG 0.465422 2_4:-2650000 None:intergenic
TTTACCCCAAAGGTTCGTTC+TGG 0.466485 2_4:+2650859 Msa0308420:CDS
ATTGATGATGGATATCTCAA+TGG 0.476570 2_4:+2651369 Msa0308420:CDS
AGTTTCCAACACCATCTTCA+AGG 0.478743 2_4:-2649916 None:intergenic
CGCAAGAGAGTGGAAGAGTT+GGG 0.482910 2_4:+2651339 Msa0308420:CDS
AGCTCTCTGGCACTTTGCTG+TGG 0.489801 2_4:-2651603 None:intergenic
CATTTATCAACAAAGCGTGT+TGG 0.491091 2_4:+2649944 Msa0308420:CDS
TTGTCCGTCGCGGATTAAGA+AGG 0.491238 2_4:+2651571 Msa0308420:CDS
AATGTGACCACCTGAACATC+GGG 0.492122 2_4:-2651471 None:intergenic
ACGCAAGAGAGTGGAAGAGT+TGG 0.493312 2_4:+2651338 Msa0308420:CDS
CAAATGCCTTCCAAGTATAA+GGG 0.493925 2_4:-2651220 None:intergenic
TTAACAAACACAGCTGCATC+AGG 0.494777 2_4:-2651021 None:intergenic
TAATCAAATGCTACAAATGA+AGG 0.496441 2_4:-2652295 None:intergenic
TTCTTGATCATTGGGTGTGA+TGG 0.498472 2_4:+2651510 Msa0308420:CDS
TGCACTTCTGCTGAAGAGGT+TGG 0.500612 2_4:+2651104 Msa0308420:CDS
CTGATGCACTTCTGCTGAAG+AGG 0.500731 2_4:+2651100 Msa0308420:CDS
ACATCGGGCTCAGCGGTAAG+AGG 0.502689 2_4:-2651456 None:intergenic
TTGCGTTCTGGTAAATAACT+TGG 0.503132 2_4:-2651321 None:intergenic
TTGGGTGGTTATATTGATGA+TGG 0.506401 2_4:+2651357 Msa0308420:CDS
TATTGGTCCGAGGGGATCAA+TGG 0.507289 2_4:+2650894 Msa0308420:CDS
TGAAACCTCTCTCTTTGAAT+CGG 0.507742 2_4:+2650010 Msa0308420:CDS
AGAGGATAGATGGAGAGAGT+TGG 0.517447 2_4:+2651903 Msa0308420:three_prime_UTR
AGTTGAGTGATCGTATGAAC+CGG 0.520130 2_4:+2652155 Msa0308420:three_prime_UTR
CATTGGGTGTGATGGCATCT+GGG 0.520718 2_4:+2651518 Msa0308420:CDS
GATGATCTAGCTGCCCACCT+TGG 0.526416 2_4:+2650937 Msa0308420:CDS
CTGTGCTGATATTGGTCCGA+GGG 0.531833 2_4:+2650885 Msa0308420:CDS
GCTCTCTGGCACTTTGCTGT+GGG 0.534603 2_4:-2651602 None:intergenic
CTGGGATGTAATGTCAAGCC+AGG 0.541505 2_4:+2651536 Msa0308420:CDS
GATTAAAGAATCTATTATAG+AGG 0.543374 2_4:+2650822 Msa0308420:CDS
TGTTGCAATGTTTACCCCAA+AGG 0.546643 2_4:+2650849 Msa0308420:CDS
CGTAACCTTGAAGATGGTGT+TGG 0.550307 2_4:+2649911 Msa0308420:exon
TTGAATCGTGTCCACCCCAT+AGG 0.554769 2_4:+2651706 Msa0308420:CDS
CAATCGCGTAACCTTGAAGA+TGG 0.555592 2_4:+2649905 Msa0308420:exon
CGCTGAGCCCGATGTTCAGG+TGG 0.565779 2_4:+2651464 Msa0308420:CDS
TACTCCACGCTTACAAAGAA+CGG 0.566079 2_4:-2651271 None:intergenic
TTTACCAGAACGCAAGAGAG+TGG 0.568025 2_4:+2651329 Msa0308420:CDS
CGAGCCGTTCTTTGTAAGCG+TGG 0.568953 2_4:+2651267 Msa0308420:CDS
TACTCTTGTTTAACTTACTG+AGG 0.577309 2_4:+2651933 Msa0308420:three_prime_UTR
TAAGATTATCTGTTGTGTGT+AGG 0.581268 2_4:-2651644 None:intergenic
TGACTGCCCTTATACTTGGA+AGG 0.581529 2_4:+2651214 Msa0308420:CDS
TCATCATCCATTGATCCCCT+CGG 0.588284 2_4:-2650901 None:intergenic
AGCTTGCTCTATGTCTGAAG+AGG 0.588966 2_4:+2651740 Msa0308420:CDS
ATAAGAGATGATTTAGACTC+GGG 0.592092 2_4:+2652069 Msa0308420:three_prime_UTR
GCATACCGATTCAAAGAGAG+AGG 0.593806 2_4:-2650015 None:intergenic
CTGAATCTTCGCCTATGGGG+TGG 0.594350 2_4:-2651717 None:intergenic
GGACAAGACTAACTGCATCC+TGG 0.595749 2_4:-2651554 None:intergenic
CAATGTGACCACCTGAACAT+CGG 0.599254 2_4:-2651472 None:intergenic
CAGAGTATTAGCAGTTCATG+TGG 0.601084 2_4:+2651180 Msa0308420:CDS
ATTTCTCGATTTCGATTTGG+GGG 0.601287 2_4:-2649873 None:intergenic
GGTATTTCAACACAAATCCA+AGG 0.613841 2_4:-2650954 None:intergenic
AAGAGGCTAGAAACAAATTG+AGG 0.621192 2_4:+2651757 Msa0308420:CDS
AATTTCTCGATTTCGATTTG+GGG 0.626159 2_4:-2649874 None:intergenic
AATAAGAGATGATTTAGACT+CGG 0.627339 2_4:+2652068 Msa0308420:three_prime_UTR
ACTCATCCCACAACAAAACA+AGG 0.630398 2_4:-2652131 None:intergenic
AGAGGTTGGAAGATTCTCAT+CGG 0.630866 2_4:+2651118 Msa0308420:CDS
GCTGTGCTGATATTGGTCCG+AGG 0.631517 2_4:+2650884 Msa0308420:CDS
ACTGCTAATACTCTGCTCAT+CGG 0.645620 2_4:-2651172 None:intergenic
CACCTGAACATCGGGCTCAG+CGG 0.649950 2_4:-2651463 None:intergenic
GGGCTCAGCGGTAAGAGGTG+AGG 0.652622 2_4:-2651451 None:intergenic
AGAGTATTAGCAGTTCATGT+GGG 0.653257 2_4:+2651181 Msa0308420:CDS
TTTCAACACAAATCCAAGGT+GGG 0.656005 2_4:-2650950 None:intergenic
CGTGCCTTCTTAATCCGCGA+CGG 0.659309 2_4:-2651575 None:intergenic
TGGTCATGAAGCTCATGTGA+AGG 0.675857 2_4:-2652213 None:intergenic
GAAAATGCAAACAAACAATG+TGG 0.679202 2_4:-2652102 None:intergenic
GCTCTGAATCTTCGCCTATG+GGG 0.681072 2_4:-2651720 None:intergenic
TGGGACTTAAAATTACCACT+CGG 0.684433 2_4:+2651423 Msa0308420:CDS
ATTTCAACACAAATCCAAGG+TGG 0.687818 2_4:-2650951 None:intergenic
GCAGTTAGTCTTGTCCGTCG+CGG 0.693318 2_4:+2651561 Msa0308420:CDS
CGGAATCAGATGAAACGCAG+AGG 0.697607 2_4:-2649980 None:intergenic
GCTACCAGAACGAACCTTTG+GGG 0.700429 2_4:-2650863 None:intergenic
TGTGCTGATATTGGTCCGAG+GGG 0.707211 2_4:+2650886 Msa0308420:CDS
TTAGACTGTGAGTTGCTCAG+AGG 0.708870 2_4:+2650798 Msa0308420:intron
AAGAGAGTGGAAGAGTTGGG+TGG 0.713278 2_4:+2651342 Msa0308420:CDS
TCAGGTGGTCACATTGACTG+AGG 0.718483 2_4:+2651479 Msa0308420:CDS
AGAGGTGAGGCAGAACCGAG+TGG 0.729223 2_4:-2651438 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTTCAAAATTTTGTAT+TGG + chr2_4:2650077-2650096 Msa0308420:intron 15.0%
!!! TTGTTTCAAAATTTTGTATT+GGG + chr2_4:2650078-2650097 Msa0308420:intron 15.0%
!!! TTTGTATTTTTGTTTGTTTT+TGG + chr2_4:2650163-2650182 Msa0308420:intron 15.0%
!!! TGTTGATTTGAATATGAATT+AGG + chr2_4:2650205-2650224 Msa0308420:intron 20.0%
!! GATTAAAGAATCTATTATAG+AGG + chr2_4:2650822-2650841 Msa0308420:CDS 20.0%
!! AACTTTATAAATTCAGTGTT+TGG - chr2_4:2652236-2652255 None:intergenic 20.0%
!! TGCTTCATATCAAATTTGTT+TGG + chr2_4:2650035-2650054 Msa0308420:intron 25.0%
!! GCTTCATATCAAATTTGTTT+GGG + chr2_4:2650036-2650055 Msa0308420:intron 25.0%
!! TTTGATTATTGAGCTTTGAT+CGG + chr2_4:2650102-2650121 Msa0308420:intron 25.0%
!! AAATTTGAGAATTGGTTTAG+TGG + chr2_4:2650129-2650148 Msa0308420:intron 25.0%
!! AATTTGAGAATTGGTTTAGT+GGG + chr2_4:2650130-2650149 Msa0308420:intron 25.0%
!!! GTTTTTGGATGATTTTGTAT+CGG + chr2_4:2650178-2650197 Msa0308420:intron 25.0%
! TATTGAGTTCATTTGTTTGT+TGG + chr2_4:2650326-2650345 Msa0308420:intron 25.0%
! GAGAATAAAACATTTAAGCT+TGG - chr2_4:2650460-2650479 None:intergenic 25.0%
!! TTCAATTGATTCATGATGAT+AGG + chr2_4:2650527-2650546 Msa0308420:intron 25.0%
! AGATATAGAGATTAATGAGT+TGG + chr2_4:2650591-2650610 Msa0308420:intron 25.0%
!! TAATGAGTTGGAACAAAATA+TGG + chr2_4:2650603-2650622 Msa0308420:intron 25.0%
! TTGGAACAAAATATGGTTTA+TGG + chr2_4:2650610-2650629 Msa0308420:intron 25.0%
!! TTAAATCCCTTTAGTAGTTT+TGG + chr2_4:2650730-2650749 Msa0308420:intron 25.0%
! ATACAACCAAAACTACTAAA+GGG - chr2_4:2650739-2650758 None:intergenic 25.0%
! AATACAACCAAAACTACTAA+AGG - chr2_4:2650740-2650759 None:intergenic 25.0%
!!! TTTTGAAGATACTACTATGA+TGG + chr2_4:2651065-2651084 Msa0308420:CDS 25.0%
! CAGAATTGATTAATGTTTGT+AGG + chr2_4:2651835-2651854 Msa0308420:three_prime_UTR 25.0%
!!! AGGCATTTTCTAGTTTATTT+TGG + chr2_4:2651953-2651972 Msa0308420:three_prime_UTR 25.0%
! AATAAGAGATGATTTAGACT+CGG + chr2_4:2652068-2652087 Msa0308420:three_prime_UTR 25.0%
! TCGAATAAAAAAGATTGAAC+CGG - chr2_4:2652177-2652196 None:intergenic 25.0%
! AATTTCTCGATTTCGATTTG+GGG - chr2_4:2649877-2649896 None:intergenic 30.0%
GAATTTCTCGATTTCGATTT+GGG - chr2_4:2649878-2649897 None:intergenic 30.0%
TGAATTTCTCGATTTCGATT+TGG - chr2_4:2649879-2649898 None:intergenic 30.0%
TTGTAGCTTGAATTTCGAAT+TGG + chr2_4:2650392-2650411 Msa0308420:intron 30.0%
TGTAGCTTGAATTTCGAATT+GGG + chr2_4:2650393-2650412 Msa0308420:intron 30.0%
! ATTTCGAATTGGGTGTTTTT+AGG + chr2_4:2650403-2650422 Msa0308420:intron 30.0%
!!! TTAGGTTTTGTTCATTCTCT+TGG + chr2_4:2650421-2650440 Msa0308420:intron 30.0%
TGGTTTATGGTAGAACAATA+TGG + chr2_4:2650623-2650642 Msa0308420:intron 30.0%
!!! TTGATGTTGTTTTAGCTTAG+TGG + chr2_4:2650675-2650694 Msa0308420:intron 30.0%
!! AAAACAGCATAAAAAGCACT+CGG - chr2_4:2650985-2651004 None:intergenic 30.0%
!!! GCTTTTTATGCTGTTTTTGA+TGG + chr2_4:2650988-2651007 Msa0308420:CDS 30.0%
!! ATTGATGATGGATATCTCAA+TGG + chr2_4:2651369-2651388 Msa0308420:CDS 30.0%
TAAGATTATCTGTTGTGTGT+AGG - chr2_4:2651647-2651666 None:intergenic 30.0%
! TACTCTTGTTTAACTTACTG+AGG + chr2_4:2651933-2651952 Msa0308420:three_prime_UTR 30.0%
!!! CTAGTTTATTTTGGCATTTG+TGG + chr2_4:2651962-2651981 Msa0308420:three_prime_UTR 30.0%
!! AATGTAGCAAAAGCTAGTTT+TGG + chr2_4:2651997-2652016 Msa0308420:three_prime_UTR 30.0%
ATAAGAGATGATTTAGACTC+GGG + chr2_4:2652069-2652088 Msa0308420:three_prime_UTR 30.0%
GAAAATGCAAACAAACAATG+TGG - chr2_4:2652105-2652124 None:intergenic 30.0%
GGGGGTTTGAAGAAAATTTA+GGG - chr2_4:2649858-2649877 None:intergenic 35.0%
TGGGGGTTTGAAGAAAATTT+AGG - chr2_4:2649859-2649878 None:intergenic 35.0%
!! ATTTCTCGATTTCGATTTGG+GGG - chr2_4:2649876-2649895 None:intergenic 35.0%
CATTTATCAACAAAGCGTGT+TGG + chr2_4:2649944-2649963 Msa0308420:CDS 35.0%
TGAAACCTCTCTCTTTGAAT+CGG + chr2_4:2650010-2650029 Msa0308420:CDS 35.0%
TCGGTGGAAAATTTGAGAAT+TGG + chr2_4:2650121-2650140 Msa0308420:intron 35.0%
!! TTGGATGATTTTGTATCGGT+CGG + chr2_4:2650182-2650201 Msa0308420:intron 35.0%
! GTTTTGTGAGTTTCATGTGT+TGG + chr2_4:2650227-2650246 Msa0308420:intron 35.0%
TTTCAACACAAATCCAAGGT+GGG - chr2_4:2650953-2650972 None:intergenic 35.0%
ATTTCAACACAAATCCAAGG+TGG - chr2_4:2650954-2650973 None:intergenic 35.0%
GGTATTTCAACACAAATCCA+AGG - chr2_4:2650957-2650976 None:intergenic 35.0%
!!! TATGCTGTTTTTGATGGACA+TGG + chr2_4:2650994-2651013 Msa0308420:CDS 35.0%
! AGAGTATTAGCAGTTCATGT+GGG + chr2_4:2651181-2651200 Msa0308420:CDS 35.0%
CAAATGCCTTCCAAGTATAA+GGG - chr2_4:2651223-2651242 None:intergenic 35.0%
TTGCGTTCTGGTAAATAACT+TGG - chr2_4:2651324-2651343 None:intergenic 35.0%
TTGGGTGGTTATATTGATGA+TGG + chr2_4:2651357-2651376 Msa0308420:CDS 35.0%
TGGGACTTAAAATTACCACT+CGG + chr2_4:2651423-2651442 Msa0308420:CDS 35.0%
AAGACGAGTTCTTGATCATT+GGG + chr2_4:2651502-2651521 Msa0308420:CDS 35.0%
AAGAGGCTAGAAACAAATTG+AGG + chr2_4:2651757-2651776 Msa0308420:CDS 35.0%
TTCCATTCCTACAAAACTTC+TGG - chr2_4:2651884-2651903 None:intergenic 35.0%
!! AAGTTTTGTAGGAATGGAAG+AGG + chr2_4:2651885-2651904 Msa0308420:three_prime_UTR 35.0%
!!! TTAGCTCCTTGTTTTGTTGT+GGG + chr2_4:2652125-2652144 Msa0308420:three_prime_UTR 35.0%
AGTTTCCAACACCATCTTCA+AGG - chr2_4:2649919-2649938 None:intergenic 40.0%
GAGAGAGGTTTCAGTTGAAA+CGG - chr2_4:2650003-2650022 None:intergenic 40.0%
! GATTATTGAGCTTTGATCGG+TGG + chr2_4:2650105-2650124 Msa0308420:intron 40.0%
! TGAGAATTGGTTTAGTGGGT+CGG + chr2_4:2650134-2650153 Msa0308420:intron 40.0%
ACAATATGGCGTAGTTTGAG+AGG + chr2_4:2650637-2650656 Msa0308420:intron 40.0%
TGTTGCAATGTTTACCCCAA+AGG + chr2_4:2650849-2650868 Msa0308420:CDS 40.0%
!! CATAAAAAGCACTCGGTGTA+GGG - chr2_4:2650978-2650997 None:intergenic 40.0%
TTAACAAACACAGCTGCATC+AGG - chr2_4:2651024-2651043 None:intergenic 40.0%
AGAGGTTGGAAGATTCTCAT+CGG + chr2_4:2651118-2651137 Msa0308420:CDS 40.0%
! TCTCATCGGAAAGCTTTTCT+AGG + chr2_4:2651132-2651151 Msa0308420:CDS 40.0%
!!! TCTAGGTGCTGACCTTTTAT+TGG + chr2_4:2651149-2651168 Msa0308420:CDS 40.0%
ACTGCTAATACTCTGCTCAT+CGG - chr2_4:2651175-2651194 None:intergenic 40.0%
! CAGAGTATTAGCAGTTCATG+TGG + chr2_4:2651180-2651199 Msa0308420:CDS 40.0%
GCAAATGCCTTCCAAGTATA+AGG - chr2_4:2651224-2651243 None:intergenic 40.0%
!! TATACTTGGAAGGCATTTGC+TGG + chr2_4:2651224-2651243 Msa0308420:CDS 40.0%
TACTCCACGCTTACAAAGAA+CGG - chr2_4:2651274-2651293 None:intergenic 40.0%
!! TTTTAAGTCCCAATCGCCAA+GGG - chr2_4:2651415-2651434 None:intergenic 40.0%
!! ATTTTAAGTCCCAATCGCCA+AGG - chr2_4:2651416-2651435 None:intergenic 40.0%
GAAGACGAGTTCTTGATCAT+TGG + chr2_4:2651501-2651520 Msa0308420:CDS 40.0%
TTCTTGATCATTGGGTGTGA+TGG + chr2_4:2651510-2651529 Msa0308420:CDS 40.0%
!! AGCCAGAAGTTTTGTAGGAA+TGG + chr2_4:2651879-2651898 Msa0308420:three_prime_UTR 40.0%
TAGGAATGGAAGAGGATAGA+TGG + chr2_4:2651893-2651912 Msa0308420:three_prime_UTR 40.0%
!!! GTAGCAAAAGCTAGTTTTGG+CGG + chr2_4:2652000-2652019 Msa0308420:three_prime_UTR 40.0%
!!! CTTAGCTCCTTGTTTTGTTG+TGG + chr2_4:2652124-2652143 Msa0308420:three_prime_UTR 40.0%
ACTCATCCCACAACAAAACA+AGG - chr2_4:2652134-2652153 None:intergenic 40.0%
AGTTGAGTGATCGTATGAAC+CGG + chr2_4:2652155-2652174 Msa0308420:three_prime_UTR 40.0%
CAATCGCGTAACCTTGAAGA+TGG + chr2_4:2649905-2649924 Msa0308420:exon 45.0%
CGTAACCTTGAAGATGGTGT+TGG + chr2_4:2649911-2649930 Msa0308420:exon 45.0%
!! GCATACCGATTCAAAGAGAG+AGG - chr2_4:2650018-2650037 None:intergenic 45.0%
ATATGGCGTAGTTTGAGAGG+TGG + chr2_4:2650640-2650659 Msa0308420:intron 45.0%
TATGGCGTAGTTTGAGAGGT+GGG + chr2_4:2650641-2650660 Msa0308420:intron 45.0%
TTAGACTGTGAGTTGCTCAG+AGG + chr2_4:2650798-2650817 Msa0308420:intron 45.0%
TTTACCCCAAAGGTTCGTTC+TGG + chr2_4:2650859-2650878 Msa0308420:CDS 45.0%
AGCTACCAGAACGAACCTTT+GGG - chr2_4:2650867-2650886 None:intergenic 45.0%
! TCTGGTAGCTGTGCTGATAT+TGG + chr2_4:2650877-2650896 Msa0308420:CDS 45.0%
TCATCATCCATTGATCCCCT+CGG - chr2_4:2650904-2650923 None:intergenic 45.0%
!! GCATAAAAAGCACTCGGTGT+AGG - chr2_4:2650979-2650998 None:intergenic 45.0%
!!! GCTGTTTTTGATGGACATGG+AGG + chr2_4:2650997-2651016 Msa0308420:CDS 45.0%
TCTGCTCATCGGCCAATAAA+AGG - chr2_4:2651164-2651183 None:intergenic 45.0%
TGACTGCCCTTATACTTGGA+AGG + chr2_4:2651214-2651233 Msa0308420:CDS 45.0%
! CATTTGCTGGTTGCTAATGC+TGG + chr2_4:2651237-2651256 Msa0308420:CDS 45.0%
TTTACCAGAACGCAAGAGAG+TGG + chr2_4:2651329-2651348 Msa0308420:CDS 45.0%
AATGTGACCACCTGAACATC+GGG - chr2_4:2651474-2651493 None:intergenic 45.0%
CAATGTGACCACCTGAACAT+CGG - chr2_4:2651475-2651494 None:intergenic 45.0%
TGCTTCTTTGACAAGCTCTC+TGG - chr2_4:2651619-2651638 None:intergenic 45.0%
!! AGCTCTGAATCTTCGCCTAT+GGG - chr2_4:2651724-2651743 None:intergenic 45.0%
!! AAGCTCTGAATCTTCGCCTA+TGG - chr2_4:2651725-2651744 None:intergenic 45.0%
! AGCTTGCTCTATGTCTGAAG+AGG + chr2_4:2651740-2651759 Msa0308420:CDS 45.0%
!! AGAGCAGCCAGAAGTTTTGT+AGG + chr2_4:2651874-2651893 Msa0308420:three_prime_UTR 45.0%
AGAGGATAGATGGAGAGAGT+TGG + chr2_4:2651903-2651922 Msa0308420:three_prime_UTR 45.0%
TGGTCATGAAGCTCATGTGA+AGG - chr2_4:2652216-2652235 None:intergenic 45.0%
CGGAATCAGATGAAACGCAG+AGG - chr2_4:2649983-2650002 None:intergenic 50.0%
TTGAGAGGTGGGATGAGACT+TGG + chr2_4:2650652-2650671 Msa0308420:intron 50.0%
GCTACCAGAACGAACCTTTG+GGG - chr2_4:2650866-2650885 None:intergenic 50.0%
CAGCTACCAGAACGAACCTT+TGG - chr2_4:2650868-2650887 None:intergenic 50.0%
! CTGTGCTGATATTGGTCCGA+GGG + chr2_4:2650885-2650904 Msa0308420:CDS 50.0%
! TGTGCTGATATTGGTCCGAG+GGG + chr2_4:2650886-2650905 Msa0308420:CDS 50.0%
TATTGGTCCGAGGGGATCAA+TGG + chr2_4:2650894-2650913 Msa0308420:CDS 50.0%
CTGATGCACTTCTGCTGAAG+AGG + chr2_4:2651100-2651119 Msa0308420:CDS 50.0%
TGCACTTCTGCTGAAGAGGT+TGG + chr2_4:2651104-2651123 Msa0308420:CDS 50.0%
!! GCACTGACTGCCCTTATACT+TGG + chr2_4:2651210-2651229 Msa0308420:CDS 50.0%
TCTTCCACTCTCTTGCGTTC+TGG - chr2_4:2651336-2651355 None:intergenic 50.0%
ACGCAAGAGAGTGGAAGAGT+TGG + chr2_4:2651338-2651357 Msa0308420:CDS 50.0%
CGCAAGAGAGTGGAAGAGTT+GGG + chr2_4:2651339-2651358 Msa0308420:CDS 50.0%
AAGAGAGTGGAAGAGTTGGG+TGG + chr2_4:2651342-2651361 Msa0308420:CDS 50.0%
! TCAGGTGGTCACATTGACTG+AGG + chr2_4:2651479-2651498 Msa0308420:CDS 50.0%
TCATTGGGTGTGATGGCATC+TGG + chr2_4:2651517-2651536 Msa0308420:CDS 50.0%
CATTGGGTGTGATGGCATCT+GGG + chr2_4:2651518-2651537 Msa0308420:CDS 50.0%
CTGGGATGTAATGTCAAGCC+AGG + chr2_4:2651536-2651555 Msa0308420:CDS 50.0%
GGACAAGACTAACTGCATCC+TGG - chr2_4:2651557-2651576 None:intergenic 50.0%
! TTGTCCGTCGCGGATTAAGA+AGG + chr2_4:2651571-2651590 Msa0308420:CDS 50.0%
TTGAATCGTGTCCACCCCAT+AGG + chr2_4:2651706-2651725 Msa0308420:CDS 50.0%
! GCTCTGAATCTTCGCCTATG+GGG - chr2_4:2651723-2651742 None:intergenic 50.0%
GGATAGATGGAGAGAGTTGG+TGG + chr2_4:2651906-2651925 Msa0308420:three_prime_UTR 50.0%
!!! AGCTAGTTTTGGCGGTACTG+TGG + chr2_4:2652008-2652027 Msa0308420:three_prime_UTR 50.0%
! GCTGTGCTGATATTGGTCCG+AGG + chr2_4:2650884-2650903 Msa0308420:CDS 55.0%
GATGATCTAGCTGCCCACCT+TGG + chr2_4:2650937-2650956 Msa0308420:CDS 55.0%
CGAGCCGTTCTTTGTAAGCG+TGG + chr2_4:2651267-2651286 Msa0308420:CDS 55.0%
CTATCTGTCACTCGTGCCCT+TGG + chr2_4:2651396-2651415 Msa0308420:CDS 55.0%
!! GCAGTTAGTCTTGTCCGTCG+CGG + chr2_4:2651561-2651580 Msa0308420:CDS 55.0%
CGTGCCTTCTTAATCCGCGA+CGG - chr2_4:2651578-2651597 None:intergenic 55.0%
! GCTCTCTGGCACTTTGCTGT+GGG - chr2_4:2651605-2651624 None:intergenic 55.0%
AGCTCTCTGGCACTTTGCTG+TGG - chr2_4:2651606-2651625 None:intergenic 55.0%
CTGAATCTTCGCCTATGGGG+TGG - chr2_4:2651720-2651739 None:intergenic 55.0%
TCACTCGTGCCCTTGGCGAT+TGG + chr2_4:2651403-2651422 Msa0308420:CDS 60.0%
CACTCGTGCCCTTGGCGATT+GGG + chr2_4:2651404-2651423 Msa0308420:CDS 60.0%
AGAGGTGAGGCAGAACCGAG+TGG - chr2_4:2651441-2651460 None:intergenic 60.0%
ACATCGGGCTCAGCGGTAAG+AGG - chr2_4:2651459-2651478 None:intergenic 60.0%
TACCGCTGAGCCCGATGTTC+AGG + chr2_4:2651461-2651480 Msa0308420:CDS 60.0%
CACCTGAACATCGGGCTCAG+CGG - chr2_4:2651466-2651485 None:intergenic 60.0%
GGGCTCAGCGGTAAGAGGTG+AGG - chr2_4:2651454-2651473 None:intergenic 65.0%
CGCTGAGCCCGATGTTCAGG+TGG + chr2_4:2651464-2651483 Msa0308420:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 2649825 2652309 2649825 ID=Msa0308420;Name=Msa0308420
chr2_4 mRNA 2649825 2652309 2649825 ID=Msa0308420-mRNA-1;Parent=Msa0308420;Name=Msa0308420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=99|1|1|1|1|1|2|509|368
chr2_4 exon 2649825 2650031 2649825 ID=Msa0308420-mRNA-1:exon:679;Parent=Msa0308420-mRNA-1
chr2_4 exon 2650802 2652309 2650802 ID=Msa0308420-mRNA-1:exon:680;Parent=Msa0308420-mRNA-1
chr2_4 five_prime_UTR 2649825 2649923 2649825 ID=Msa0308420-mRNA-1:five_prime_utr;Parent=Msa0308420-mRNA-1
chr2_4 CDS 2649924 2650031 2649924 ID=Msa0308420-mRNA-1:cds;Parent=Msa0308420-mRNA-1
chr2_4 CDS 2650802 2651800 2650802 ID=Msa0308420-mRNA-1:cds;Parent=Msa0308420-mRNA-1
chr2_4 three_prime_UTR 2651801 2652309 2651801 ID=Msa0308420-mRNA-1:three_prime_utr;Parent=Msa0308420-mRNA-1
Gene Sequence

>Msa0308420

ATGGTGTTGGAAACTGAAATCATTTATCAACAAAGCGTGTTGGATGTTAAGTATCACCTCTGCGTTTCATCTGATTCCGTTTCAACTGAAACCTCTCTCTTTGAATCGACTGTGAGTTGCTCAGAGGTGATTAAAGAATCTATTATAGAGGATGCTGTTGCAATGTTTACCCCAAAGGTTCGTTCTGGTAGCTGTGCTGATATTGGTCCGAGGGGATCAATGGATGATGAACACATTCAAATAGATGATCTAGCTGCCCACCTTGGATTTGTGTTGAAATACCCTACACCGAGTGCTTTTTATGCTGTTTTTGATGGACATGGAGGTCCTGATGCAGCTGTGTTTGTTAAGAACAATGCTATGAAATTATTTTTTGAAGATACTACTATGATGGAAACGTATGACTCTGATGCACTTCTGCTGAAGAGGTTGGAAGATTCTCATCGGAAAGCTTTTCTAGGTGCTGACCTTTTATTGGCCGATGAGCAGAGTATTAGCAGTTCATGTGGGACTACAGCACTGACTGCCCTTATACTTGGAAGGCATTTGCTGGTTGCTAATGCTGGTGATTGTCGAGCCGTTCTTTGTAAGCGTGGAGTAGCTGTTGACATGTCTCAAGATCATAGACCAAGTTATTTACCAGAACGCAAGAGAGTGGAAGAGTTGGGTGGTTATATTGATGATGGATATCTCAATGGTTATCTATCTGTCACTCGTGCCCTTGGCGATTGGGACTTAAAATTACCACTCGGTTCTGCCTCACCTCTTACCGCTGAGCCCGATGTTCAGGTGGTCACATTGACTGAGGAAGACGAGTTCTTGATCATTGGGTGTGATGGCATCTGGGATGTAATGTCAAGCCAGGATGCAGTTAGTCTTGTCCGTCGCGGATTAAGAAGGCACGATGACCCACAGCAAAGTGCCAGAGAGCTTGTCAAAGAAGCATTGCGCCTACACACAACAGATAATCTTACTGTAATTGTCATTTGCTTGTCTGCTGTCGAAAGCATTGTTGAATCGTGTCCACCCCATAGGCGAAGATTCAGAGCTTGCTCTATGTCTGAAGAGGCTAGAAACAAATTGAGGAGCTTGCTAGAAAGTAACTGA
Protein sequence

>Msa0308420

MVLETEIIYQQSVLDVKYHLCVSSDSVSTETSLFESTVSCSEVIKESIIEDAVAMFTPKVRSGSCADIGPRGSMDDEHIQIDDLAAHLGFVLKYPTPSAFYAVFDGHGGPDAAVFVKNNAMKLFFEDTTMMETYDSDALLLKRLEDSHRKAFLGADLLLADEQSISSSCGTTALTALILGRHLLVANAGDCRAVLCKRGVAVDMSQDHRPSYLPERKRVEELGGYIDDGYLNGYLSVTRALGDWDLKLPLGSASPLTAEPDVQVVTLTEEDEFLIIGCDGIWDVMSSQDAVSLVRRGLRRHDDPQQSARELVKEALRLHTTDNLTVIVICLSAVESIVESCPPHRRRFRACSMSEEARNKLRSLLESN*